scholarly journals Recurrent Selection with Sub-Lethal Doses of Mesotrione Reduces Sensitivity in Amaranthus palmeri

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1293
Author(s):  
Jason K. Norsworthy ◽  
Vijay K. Varanasi ◽  
Muthukumar Bagavathiannan ◽  
Chad Brabham
Keyword(s):  
Recurrent Selection ◽  
Selection Process ◽  
Sensitivity Index ◽  
Amaranthus Palmeri ◽  
Agricultural Field ◽  
Weed Species ◽  
Resistance Evolution ◽  
Repeated Use ◽  
Successive Generations ◽  
Sites Of Action

Amaranthus palmeri, ranked as the most prolific and troublesome weed in North America, has evolved resistance to several herbicide sites of action. Repeated use of any one herbicide, especially at lower than recommended doses, can lead to evolution of weed resistance, and, therefore, a better understanding of the process of resistance evolution is essential for the management of A. palmeri and other difficult-to-control weed species. Amaranthus palmeri rapidly developed resistance to 4-hydroxyphenylpyruvate dioxygenase (HPPD) inhibitors such as mesotrione. The objective of this study was to test the potential for low-dose applications of mesotrione to select for reduced susceptibility over multiple generations in an A. palmeri population collected from an agricultural field in 2001. F0 plants from the population were initially treated with sub-lethal mesotrione rates and evaluated for survival three weeks after treatment. All F0 plants were controlled at the 1× rate (x = 105 g ai ha−1). However, 2.5% of the F0 plants survived the 0.5× treatment. The recurrent selection process using plants surviving various mesotrione rates was continued until the F4 generation was reached. Based on the GR50 values, the sensitivity index was determined to be 1.7 for the F4 generation. Compared to F0, HPPD gene expression level in the F3 population increased. Results indicate that after several rounds of recurrent selection, the successive generations of A. palmeri became less responsive to mesotrione, which may explain the reduced sensitivity of this weed to HPPD-inhibiting herbicides. The results have significance in light of the recently released soybean and soon to be released cotton varieties with resistance to HPPD inhibitors.

scholarly journals Effect of PPO-Inhibiting Herbicides on the Growth and Sex Ratio of a Dioecious Weed Species Amaranthus palmeri (Palmer Amaranth)

Agronomy ◽  
2019 ◽  
Vol 9 (6) ◽  
pp. 275 ◽  
Author(s):  
Mafia M. Rumpa ◽  
Ronald F. Krausz ◽  
David J. Gibson ◽  
Karla L. Gage
Keyword(s):  
Sex Ratio ◽  
Seed Production ◽  
Sex Ratios ◽  
Rapid Evolution ◽  
Palmer Amaranth ◽  
Amaranthus Palmeri ◽  
Weed Species ◽  
Herbicide Treatments ◽  
Underlying Mechanisms ◽  
Sites Of Action

Amaranthus palmeri S. Watson (Palmer amaranth) is a fast-growing, dioecious, highly competitive agricultural weed species, which is spreading across the US Midwest. Population sex ratios are an important consideration in the management of A. palmeri populations as this species has become resistant to several herbicide sites of action, and there is need to minimize seed production by female plants. Environmental conditions, particularly stressors, may influence sex ratios, and herbicides act as major stressors and evolutionary filters in agricultural fields. Amaranthus spp. have shown a tendency for rapid evolution of herbicide resistance, with the frequency of protoporphyrinogen oxidase (PPO)-inhibitor resistance increasing across the Midwestern US. A greenhouse experiment was conducted to investigate the effect of two PPO-inhibiting herbicide treatments of either lactofen or fomesafen on four different Illinois populations (Cahokia, Collinsville, Rend Lake, and Massac). Plants raised from seed from the Massac population were tallest, and both males and females from this population also had the highest vegetative biomass. Female plants from the Collinsville population had more reproductive biomass than male plants. Control populations were male-biased (Cahokia, Collinsville), female-biased (Masaac), and 1:1 (Rend Lake). Lactofen shifted the male-biased populations to female-biased or 1:1 and the female-biased population to 1:1. Fomesafen-treated populations were male-biased or 1:1. This study suggests that PPO-inhibiting herbicide treatments may influence the growth and sex ratio of A. palmeri populations, which is an underlying factor in the rate of herbicide evolution in this species. An understanding of the underlying mechanisms of how external factors influence sex ratios may eventually provide an opportunity to reduce seed production in populations by shifting sex ratios towards a male bias.

scholarly journals Amaranthus palmeri a New Invasive Weed in Spain with Herbicide Resistant Biotypes

Agronomy ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 993
Author(s):  
Joel Torra ◽  
Aritz Royo-Esnal ◽  
Yolanda Romano ◽  
María Dolores Osuna ◽  
Ramón G. León ◽  
...  
Keyword(s):  
Amino Acid ◽  
Agricultural Land ◽  
Acetolactate Synthase ◽  
Inter Simple Sequence Repeat ◽  
Group Method ◽  
Amaranthus Palmeri ◽  
American Continent ◽  
Weed Species ◽  
Invasive Weed ◽  
Sites Of Action

Amaranthus palmeri is the most prominent invasive weed in agricultural land from North America, partly due to its propensity to evolve resistance to multiple herbicide sites of action. In the last two decades, reports of this species have increased throughout the American continent and occasionally in other continents. In 2007, A. palmeri populations were found in three localities in northeastern Spain, and they are still present today. To determine whether these three populations resulted from a common or independent introduction events—and when and from where they could have occurred—research was carried out aiming to characterize the resistance profile and mechanisms to 5-enolpyruvylshikimate-3-phosphate synthase-and acetolactate synthase (ALS)-inhibiting herbicides and to analyze the relationship between these three populations using inter simple sequence repeat DNA fingerprinting. Dose–response trials confirmed that the three populations were susceptible to glyphosate but resistant to nicosulfuron-methyl. Resistance to ALS inhibitors was due to several amino acid substitutions in positions Pro197, Trp574 and Ser653. Moreover, the substitutions Ser653Ile and Pro197Thr are described for the first time in this species. At field-labeled rates, all populations were fully controlled with alternative herbicides with other sites of action. Amaranthus palmeri individuals were clustered in three groups based on unweighted pair group method with arithmetic mean analysis, which corresponded to the three sampled populations, with a 67% of genetic relationship among them. Considering this high genetic variability and the different positions and amino acid substations found between populations, it was hypothesized that different colonization events occurred from the American continent probably prior to the introduction of glyphosate resistant crops. Prevention from new introductions is warranted because new herbicide resistance traits could arrive, complicating the management of this invasive weed species, while managing or eradicating the already established populations.

scholarly journals An ABCC-type transporter endowing glyphosate resistance in plants

2021 ◽  
Vol 118 (16) ◽  
pp. e2100136118 ◽  
Author(s):  
Lang Pan ◽  
Qin Yu ◽  
Junzhi Wang ◽  
Heping Han ◽  
Lingfeng Mao ◽  
...  
Keyword(s):  
Plant Species ◽  
Transgenic Rice ◽  
Glyphosate Resistance ◽  
Agricultural Field ◽  
Weed Species ◽  
Vegetation Control ◽  
Resistance Evolution ◽  
Wide Range ◽  
Localization Analysis ◽  
Selection Of

Glyphosate is the most widely used herbicide in world agriculture and for general vegetation control in a wide range of situations. Global and often intensive glyphosate selection of very large weedy plant populations has resulted in widespread glyphosate resistance evolution in populations of many weed species. Here, working with a glyphosate-resistant (GR) Echinochloa colona population that evolved in a Western Australia agricultural field, we identified an ATP-binding cassette (ABC) transporter (EcABCC8) that is consistently up-regulated in GR plants. When expressed in transgenic rice, this EcABCC8 transporter endowed glyphosate resistance. Equally, rice, maize, and soybean overexpressing the EcABCC8 ortholog genes were made resistant to glyphosate. Conversely, CRISPR/Cas9-mediated knockout of the EcABCC8 ortholog gene OsABCC8 increased rice susceptibility to glyphosate. Subcellular localization analysis and quantification of glyphosate cellular levels in treated ABCC8 transgenic rice plants and isolated leaf protoplasts as well as structural modeling support that EcABCC8 is likely a plasma membrane–localized transporter extruding cytoplasmic glyphosate to the apoplast, lowering the cellular glyphosate level. This is a report of a membrane transporter effluxing glyphosate in a GR plant species, and its function is likely conserved in crop plant species.

scholarly journals Weeds Hosting the Soybean Cyst Nematode (Heterodera glycines Ichinohe): Management Implications in Agroecological Systems

Agronomy ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 146
Author(s):  
Leonardo F. Rocha ◽  
Karla L. Gage ◽  
Mirian F. Pimentel ◽  
Jason P. Bond ◽  
Ahmad M. Fakhoury
Keyword(s):  
Weed Management ◽  
Soybean Cyst Nematode ◽  
Heterodera Glycines ◽  
Integrated Management ◽  
Cropping System ◽  
Cyst Nematode ◽  
Weed Species ◽  
Initial Inoculum ◽  
Herbicide Resistant ◽  
Sites Of Action

The soybean cyst nematode (SCN; Heterodera glycines Ichinohe) is a major soybean-yield-limiting soil-borne pathogen, especially in the Midwestern US. Weed management is recommended for SCN integrated management, since some weed species have been reported to be hosts for SCN. The increase in the occurrence of resistance to herbicides complicates weed management and may further direct ecological–evolutionary (eco–evo) feedbacks in plant–pathogen complexes, including interactions between host plants and SCN. In this review, we summarize weed species reported to be hosts of SCN in the US and outline potential weed–SCN management interactions. Plants from 23 families have been reported to host SCN, with Fabaceae including most host species. Out of 116 weeds hosts, 14 species have known herbicide-resistant biotypes to 8 herbicide sites of action. Factors influencing the ability of weeds to host SCN are environmental and edaphic conditions, SCN initial inoculum, weed population levels, and variations in susceptibility of weed biotypes to SCN within a population. The association of SCN on weeds with relatively little fitness cost incurred by the latter may decrease the competitive ability of the crop and increase weed reproduction when SCN is present, feeding back into the probability of selecting for herbicide-resistant weed biotypes. Therefore, proper management of weed hosts of SCN should be a focus of integrated pest management (IPM) strategies to prevent further eco–evo feedbacks in the cropping system.

Critical time for weed removal in glyphosate-resistant soybean as influenced by preemergence herbicides

2019 ◽  
Vol 33 (03) ◽  
pp. 393-399 ◽  
Author(s):  
Stevan Z. Knezevic ◽  
Pavle Pavlovic ◽  
O. Adewale Osipitan ◽  
Ethann R. Barnes ◽  
Clint Beiermann ◽  
...  
Keyword(s):  
Field Experiments ◽  
Critical Time ◽  
Growing Season ◽  
Control Programs ◽  
Main Plot ◽  
Repeated Use ◽  
Sites Of Action ◽  
Alternative Sites ◽  
Preemergence Herbicides ◽  
Weed Removal

AbstractWidespread and repeated use of glyphosate resulted in an increase in glyphosate-resistant (GR) weeds. This led to an urgent need for diversification of weed control programs and use of PRE herbicides with alternative sites of action. Field experiments were conducted over a 4-yr period (2015 to 2018) across three locations in Nebraska to evaluate the effects of PRE-applied herbicides on critical time for weed removal (CTWR) in GR soybean. The studies were laid out in a split-plot arrangement with herbicide regime as the main plot and weed removal timing as the subplot. The herbicide regimes used were either no PRE or premix of either sulfentrazone plus imazethapyr (350 + 70 g ai ha−1) or saflufenacil plus imazethapyr plus pyroxasulfone (26 + 70 + 120 g ai ha−1). The weed removal timings were at V1, V3, V6, R2, and R5 soybean stages, with weed-free and weedy season-long checks. Weeds were removed by application of glyphosate (1,400 g ae ha−1) or by hoeing. The results across all years and locations suggested that the use of PRE herbicides delayed CTWR in soybean. In particular, the CTWR without PRE herbicides was determined to be around the V1 to V2 (14 to 21 d after emergence [DAE]) growth stage, depending on the location and weed pressure. The use of PRE-applied herbicides delayed CTWR from about the V4 (28 DAE) stage up to the R5 (66 DAE) stage. These results suggest that the use of PRE herbicides in GR soybean could delay the need for POST application of glyphosate by 2 to 5 wk, thereby reducing the need for multiple applications of glyphosate during the growing season. Additionally, the use of PRE herbicides could provide additional modes of action needed to manage GR weeds in GR soybean.

Enhanced Biodegradation of Herbicides in Soil and Effects on Weed Control

1987 ◽  
Vol 1 (4) ◽  
pp. 341-349 ◽  
Author(s):  
R. Gordon Harvey ◽  
J. H. Dekker ◽  
Richard S. Fawcett ◽  
Fred W. Roeth ◽  
Robert G. Wilson
Keyword(s):  
Weed Species ◽  
North Central ◽  
Herbicide Degradation ◽  
Proso Millet ◽  
The North ◽  
Central United States ◽  
Enhanced Biodegradation ◽  
Long Time ◽  
Previously Treated ◽  
Repeated Use

Research conducted since 1979 in the north central United States and southern Canada demonstrated that after repeated annual applications of the same thiocarbamate herbicide to the same field, control of some difficult-to-control weed species was reduced. Laboratory studies of herbicide degradation in soils from these fields indicated that these performance failures were due to more rapid or “enhanced” biodegradation of the thiocarbamate herbicides after repeated use with a shorter period during which effective herbicide levels remained in the soils. Weeds such as wild proso millet [Panicum miliaceumL. spp.ruderale(Kitagawa) Tzevelev. #3PANMI] and shattercane [Sorghum bicolor(L.) Moench. # SORVU] which germinate over long time periods were most likely to escape these herbicides after repeated use. Adding dietholate (O,O-diethylO-phenyl phosphorothioate) to EPTC (S-ethyl dipropyl carbamothioate) reduced problems caused by enhanced EPTC biodegradation in soils treated previously with EPTC alone but not in soils previously treated with EPTC plus dietholate. While previous use of other thiocarbamate herbicides frequently enhanced biodegradation of EPTC or butylate [S-ethyl bis(2-methylpropyl)carbamothioate], previous use of other classes of herbicides or the insecticide carbofuran (2,3 -dihydro-2,2 -dimethyl-7-benzofuranyl methylcarbamate) did not. Enhanced biodegradation of herbicides other than the thiocarbamates was not observed.

scholarly journals Effects of Selection to Diflubenzuron and Bacillus thuringiensis Var. Israelensis on the Overwintering Successes of Aedes albopictus (Diptera: Culicidae)

Insects ◽  
2021 ◽  
Vol 12 (9) ◽  
pp. 822
Author(s):  
Charalampos S. Ioannou ◽  
Christos Hadjichristodoulou ◽  
Varvara A. Mouchtouri ◽  
Nikos T. Papadopoulos
Keyword(s):  
Bacillus Thuringiensis ◽  
Aedes Albopictus ◽  
Selection Process ◽  
Mosquito Species ◽  
Fold Increase ◽  
Development Of Resistance ◽  
Control Programmes ◽  
Invasive Mosquito ◽  
In The Wild ◽  
Successive Generations

Aedes albopictus is an invasive mosquito species responsible for local transmission of chikungunya and dengue viruses in Europe. In the absence of available treatments, insecticides-based control remains one of the most important viable strategies to prevent emerging problems. Diflubenzuron (DFB) and Bacillus thuringiensis var. israelensis (Bti) are among the most commonly used larvicides for Ae. albopictus control with consequent concerns for the potential development of resistance. Studies on the resistance emergence in Ae. albopictus and its persistence in the wild to both DFB and Bti are essential for the efficient and sustainable planning of the control programmes. In this context, larvae from a recently laboratory established population were subjected to increasing selective pressure for nine successive generations using both DFB and Bti. The resistance levels and the overwintering success of the selected populations relative to control (colonies that received no selection) were determined. Results revealed an 8.5- and 1.6-fold increase on the resistance levels following selection with DFB and Bti, respectively. The selection process to both larvicides had no apparent impacts on the overwintering capability relative to control, suggesting the successful persistence of the selected individuals in the wild on an annual base.

Susceptibility of Palmer amaranth (Amaranthus palmeri) to herbicides in accessions collected from the North Carolina Coastal Plain

Weed Science ◽  
2020 ◽  
Vol 68 (6) ◽  
pp. 582-593
Author(s):  
Denis J. Mahoney ◽  
David L. Jordan ◽  
Nilda Roma-Burgos ◽  
Katherine M. Jennings ◽  
Ramon G. Leon ◽  
...  
Keyword(s):  
North Carolina ◽  
Dose Response ◽  
Coastal Plain ◽  
Weed Management ◽  
Acetolactate Synthase ◽  
Fold Increase ◽  
Palmer Amaranth ◽  
Amaranthus Palmeri ◽  
Sites Of Action ◽  
Two Populations

AbstractPalmer amaranth (Amaranthus palmeri S. Watson) populations resistant to acetolactate synthase (ALS)-inhibiting herbicides and glyphosate are fairly common throughout the state of North Carolina (NC). This has led farm managers to rely more heavily on herbicides with other sites of action (SOA) for A. palmeri control, especially protoporphyrinogen oxidase and glutamine synthetase inhibitors. In the fall of 2016, seeds from A. palmeri populations were collected from the NC Coastal Plain, the state’s most prominent agricultural region. In separate experiments, plants with 2 to 4 leaves from the 110 populations were treated with field use rates of glyphosate, glufosinate-ammonium, fomesafen, mesotrione, or thifensulfuron-methyl. Percent visible control and survival were evaluated 3 wk after treatment. Survival frequencies were highest following glyphosate (99%) or thifensulfuron-methyl (96%) treatment. Known mutations conferring resistance to ALS inhibitors were found in populations surviving thifensulfuron-methyl application (Ala-122-Ser, Pro-197-Ser, Trp-574-Leu, and/or Ser-653-Asn), in addition to a new mutation (Ala-282-Asp) that requires further investigation. Forty-two populations had survivors after mesotrione application, with one population having 17% survival. Four populations survived fomesafen treatment, while none survived glufosinate. Dose–response studies showed an increase in fomesafen needed to kill 50% of two populations (LD50); however, these rates were far below the field use rate (less than 5 g ha−1). In two populations following mesotrione dose–response studies, a 2.4- to 3.3-fold increase was noted, with LD90 values approaching the field use rate (72.8 and 89.8 g ha−1). Screening of the progeny of individuals surviving mesotrione confirmed the presence of resistance alleles, as there were a higher number of survivors at the 1X rate compared with the parent population, confirming resistance to mesotrione. These data suggest A. palmeri resistant to chemistries other than glyphosate and thifensulfuron-methyl are present in NC, which highlights the need for weed management approaches to mitigate the evolution and spread of herbicide-resistant populations.

RECURRENT SELECTION FOR FORAGE AND SEED YIELD IN ZIGZAG CLOVER

1984 ◽  
Vol 64 (1) ◽  
pp. 119-130 ◽  
Author(s):  
NORMAN L. TAYLOR ◽  
PAUL L. CORNELIUS ◽  
ROY E. SIGAFUS
Keyword(s):  
Recurrent Selection ◽  
Selection Process ◽  
Linear Increase ◽  
Rate Of Change ◽  
Forage Yield ◽  
Annual Basis ◽  
Phenotypic Recurrent Selection ◽  
Winter Temperatures ◽  
Selection For ◽  
Efficiency Of Selection

Phenotypic recurrent selection on an annual basis was conducted with zigzag clover (Trifolium medium L.) to determine the efficiency of selection for improved seed and forage yield and the relationships among these characters. Seedlings were exposed to winter temperatures in a cold frame before they were transplanted in a field in the spring. The undesirable types were mowed prior to flowering and the selected plants were allowed to cross. Heads and seeds were harvested from each plant, and after seed threshing and counting, plants were further selected on the basis of numbers of seeds and heads. The effectiveness of four cycles of selection was evaluated over a 2-yr period in an experiment initiated with remnant seed and with management similar to that imposed during the selection process. Recurrent selection was effective in increasing vigor, seeds per head, heads per plant, and seeds per plant. A significant linear increase over cycles was found for each character. Vigor was not correlated with seeds per head, but was correlated with the other characters. The more vigorous plants also exhibited less stand reduction. Data from the two evaluation years were correlated suggesting that materials selected on an annual basis performed similarly to those grown in a 2-yr stand. Although recurrent selection was effective for improving all traits, the rate of change, particularly for seeds per head, was limited. Many more cycles of such selection would be required to change zigzag clover into a useful forage species. It was suggested that the phenotypic recurrent selection program should be continued on an annual basis, but modified if possible to select against the vernalization requirement for flowering which may be associated with poor seedling growth and low aftermath vigor.Key words: Trifolium medium, recurrent selection, vigor, persistance, heads and seeds per plant

Absorption, Translocation, and Metabolism of Glufosinate in Transgenic and Nontransgenic Cotton, Palmer Amaranth (Amaranthus palmeri), and Pitted Morningglory (Ipomoea lacunosa)

Weed Science ◽  
2009 ◽  
Vol 57 (4) ◽  
pp. 357-361 ◽  
Author(s):  
Wesley J. Everman ◽  
Walter E. Thomas ◽  
James D. Burton ◽  
Alan C. York ◽  
John W. Wilcut
Keyword(s):  
Palmer Amaranth ◽  
Amaranthus Palmeri ◽  
Weed Species ◽  
Ipomoea Lacunosa ◽  
Leaf Stage ◽  
Intermediate Metabolism ◽  
Pitted Morningglory ◽  
Cotton Plants ◽  
Treated Leaf ◽  
Harvest Intervals

Greenhouse studies were conducted to evaluate absorption, translocation, and metabolism of14C-glufosinate in glufosinate-resistant cotton, nontransgenic cotton, Palmer amaranth, and pitted morningglory. Cotton plants were treated at the four-leaf stage, whereas Palmer amaranth and pitted morningglory were treated at 7.5 and 10 cm, respectively. All plants were harvested at 1, 6, 24, 48, and 72 h after treatment (HAT). Absorption of14C-glufosinate was greater than 85% 24 h after treatment in Palmer amaranth. Absorption was less than 30% at all harvest intervals for glufosinate-resistant cotton, nontransgenic cotton, and pitted morningglory. At 24 HAT, 49 and 12% of radioactivity was translocated to regions above and below the treated leaf, respectively, in Palmer amaranth. Metabolites of14C-glufosinate were detected in all crop and weed species. Metabolism of14C-glufosinate was 16% or lower in nontransgenic cotton and pitted morningglory; however, metabolism rates were greater than 70% in glufosinate-resistant cotton 72 HAT. Intermediate metabolism was observed for Palmer amaranth, with metabolites comprising 20 to 30% of detectable radioactivity between 6 and 72 HAT.

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