Overexpression of Rice OsS1Fa1 Gene Confers Drought Tolerance in Arabidopsis

Small peptides and proteins play critical regulatory roles in plant development and environmental stress responses; however, only a few of these molecules have been identified and characterized to date because of their poor annotation and other experimental challenges. Here, we present that rice (Oryza sativa L.) OsS1Fa1, a small 76-amino acid protein, confers drought stress tolerance in Arabidopsis thaliana. OsS1Fa1 was highly expressed in leaf, culm, and root tissues of rice seedlings during vegetative growth and was significantly induced under drought stress. OsS1Fa1 overexpression in Arabidopsis induced the expression of selected drought-responsive genes and enhanced the survival rate of transgenic lines under drought. The proteasome inhibitor MG132 protected the OsS1Fa1 protein from degradation. Together, our data indicate that the small protein OsS1Fa1 is induced by drought and is post-translationally regulated, and the ectopic expression of OsS1Fa1 protects plants from drought stress.

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Functional Analysis of Calmodulin Binding Protein 60s in Disease Resistance Responses in Rice (Oryza Sativa L.)

10.21203/rs.3.rs-151106/v1 ◽

2021 ◽

Author(s):

Diksha Kumari ◽

Bishun Deo Prasad ◽

R. B. P. Nirala ◽

Sangita Sahni

Keyword(s):

Differential Expression ◽

Stress Responses ◽

Oryza Sativa L ◽

Binding Protein ◽

Defense Responses ◽

Infection Process ◽

Functional Domain ◽

Rice Seedlings ◽

Calmodulin Binding ◽

Defence Responses

Abstract BackgroundOccurrence and prevention of diseases have a big impact on sustainability of staple food crop like rice. The crosstalk between phytohormones and secondary messengers in host cell during infestation of pathogens play a pivotal role in defence responses. Apart from SA and JA, the role of brassinosteroids (BR) in defence responses in plants is unprecedented. The calcium signatures observed during early infection process modulates the expression of calmodulin and other Ca2+ binding protein followed by their interactions with calmodulin binding protein (CBP), which are pivotal in elucidating defence responses in plant. Numerous CBPs have been identified, which modulated stress responses with the help of CBD and other functional domain. Interestingly, Arabidopsis CBP60 (AtCBP60) family protein, SARD1, was involved in defense responses via SAR. However, no rice CBP60 (OsCBP60) has been identified in relation to pathogen infection yet. ResultsIn present investigation, 15 OsCBP60 genes were identified using BLASTP searchers using AtCBP60s as bait sequences. Expression studies showed that 3 OsCBP60s (OsCBP60_5, OsCBP60_10, and OsCBP60_15) genes were upregulated consistently in all the time point studied in rice seedlings treated with fungal (Magnaporthe oryzae) and bacterial (Xanthomonas oryzae) pathogens. Differential expression of OsCBP60s genes were observed in salicylic acid (SA), epi-brassinosteroid (EBR) and jasmonic acid (JA) treated rice seedlings. Taken together, OsCBP60_5 was found to be upregulated in both pathogens and two phytohormones (SA and EBR) treatment. ConclusionsThe differential expression of OsCBP60s genes under phytohormones and pathogens treatment suggests that these genes might be important targets for increasing biotic stress responses in rice.

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Rice lectin protein Osr40c1 imparts drought tolerance by modulating OsSAM2, OsSAP8 and chromatin-associated proteins

10.1101/2020.04.19.049288 ◽

2020 ◽

Author(s):

Salman Sahid ◽

Chandan Roy ◽

Soumitra Paul ◽

Riddhi Datta

Keyword(s):

Drought Stress ◽

Drought Tolerance ◽

Stress Responses ◽

Ectopic Expression ◽

In Planta ◽

Biotic And Abiotic Stress ◽

Polyamine Biosynthesis ◽

Protein Partners ◽

Associated Proteins ◽

Lectin Protein

AbstractLectin proteins play an important role in biotic and abiotic stress responses in plants. Although the rice lectin protein, Osr40c1, has been reported to be regulated by drought stress, the mechanism of its drought tolerance activity has not been studied so far. In this study, it has been depicted that expression of Osr40c1 gene correlates with the drought tolerance potential of various rice cultivars. Transgenic rice plants overexpressing Osr40c1 were significantly more tolerant to drought stress over the wild-type plants. Furthermore, ectopic expression of the Osr40c1 gene in tobacco yielded a similar result. Interestingly, the protein displayed a nucleo-cytoplasmic localization and was found to interact with a number of drought-responsive proteins like OsSAM2, OsSAP8, OsMNB1B, and OsH4. Fascinatingly, silencing of each of these protein partners led to drought susceptibility in the otherwise tolerant Osr40c1 expressing transgenic tobacco lines indicating that these partners were crucial for the Osr40c1-mediated drought tolerance in planta. Together, the present investigation delineated the novel role of Osr40c1 protein in imparting drought tolerance by regulating the chromatin proteins, OsMNB1B and OsH4, which presumably enables OsSAP8 to induce downstream gene expression. In addition, its interaction with OsSAM2 might induce polyamine biosynthesis thus further improving drought tolerance in plants.HighlightsA rice lectin protein, Osr40c1, plays a crucial role in imparting drought stress tolerance in plants by modulating OsSAM2 as well as the transcriptional regulators OsSAP8, OsMNB1B and OsH4.

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LncRNA TCONS_00021861 is functionally associated with drought tolerance in rice (Oryza sativa L.) via competing endogenous RNA regulation

BMC Plant Biology ◽

10.1186/s12870-021-03195-z ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Jiajia Chen ◽

Yuqing Zhong ◽

Xin Qi

Keyword(s):

Drought Stress ◽

Stress Responses ◽

Crop Production ◽

Oryza Sativa L ◽

Rna Regulation ◽

Cellular Processes ◽

Cerna Network ◽

Competing Endogenous Rnas ◽

New Perspective ◽

Indole 3 Acetic Acid

Abstract Background Water deficit is an abiotic stress that retards plant growth and destabilizes crop production. Long non coding RNAs (lncRNAs) are a class of non-coding endogenous RNAs that participate in diverse cellular processes and stress responses in plants. lncRNAs could function as competing endogenous RNAs (ceRNA) and represent a novel layer of gene regulation. However, the regulatory mechanism of lncRNAs as ceRNA in drought stress response is yet unclear. Results In this study, we performed transcriptome-wide identification of drought-responsive lncRNAs in rice. Thereafter, we constructed a lncRNA-mediated ceRNA network by analyzing competing relationships between mRNAs and lncRNAs based on ceRNA hypothesis. A drought responsive ceRNA network with 40 lncRNAs, 23 miRNAs and 103 mRNAs was obtained. Network analysis revealed TCONS_00021861/miR528-3p/YUCCA7 regulatory axis as a hub involved in drought response. The miRNA-target expression and interaction were validated by RT-qPCR and RLM-5’RACE. TCONS_00021861 showed significant positive correlation (r = 0.7102) with YUCCA7 and negative correlation with miR528-3p (r = -0.7483). Overexpression of TCONS_00021861 attenuated the repression of miR528-3p on YUCCA7, leading to increased IAA (Indole-3-acetic acid) content and auxin overproduction phenotypes. Conclusions TCONS_00021861 could regulate YUCCA7 by sponging miR528-3p, which in turn activates IAA biosynthetic pathway and confer resistance to drought stress. Our findings provide a new perspective of the regulatory roles of lncRNAs as ceRNAs in drought resistance of rice.

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Chrysanthemum DgWRKY2 Gene Enhances Tolerance to Salt Stress in Transgenic Chrysanthemum

International Journal of Molecular Sciences ◽

10.3390/ijms19072062 ◽

2018 ◽

Vol 19 (7) ◽

pp. 2062 ◽

Cited By ~ 9

Author(s):

Ling He ◽

Yin-Huan Wu ◽

Qian Zhao ◽

Bei Wang ◽

Qing-Lin Liu ◽

...

Keyword(s):

Salt Stress ◽

Sugar Content ◽

Soluble Sugar ◽

Wild Type ◽

Acid Protein ◽

Transgenic Lines ◽

High Salt Stress ◽

Transgenic Chrysanthemum ◽

Stress Related Genes ◽

Amino Acid Protein

WRKY transcription factors (TFs) play a vital part in coping with different stresses. In this study, DgWRKY2 was isolated from Dendranthema grandiflorum. The gene encodes a 325 amino acid protein, belonging to the group II WRKY family, and contains one typical WRKY domain (WRKYGQK) and a zinc finger motif (C-X4-5-C-X22-23-H-X1-H). Overexpression of DgWRKY2 in chrysanthemum enhanced tolerance to high-salt stress compared to the wild type (WT). In addition, the activities of antioxidant enzymes (superoxide dismutase (SOD), peroxidase (POD), catalase (CAT)), proline content, soluble sugar content, soluble protein content, and chlorophyll content of transgenic chrysanthemum, as well as the survival rate of the transgenic lines, were on average higher than that of the WT. On the contrary, hydrogen peroxide (H2O2), superoxide anion (O2−), and malondialdehyde (MDA) accumulation decreased compared to WT. Expression of the stress-related genes DgCAT, DgAPX, DgZnSOD, DgP5CS, DgDREB1A, and DgDREB2A was increased in the DgWRKY2 transgenic chrysanthemum compared with their expression in the WT. In conclusion, our results indicate that DgWRKY2 confers salt tolerance to transgenic chrysanthemum by enhancing antioxidant and osmotic adjustment. Therefore, this study suggests that DgWRKY2 could be used as a reserve gene for salt-tolerant plant breeding.

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Ectopic expression of PgRab7 in rice plants (Oryza sativa L.) results in differential tolerance at the vegetative and seed setting stage during salinity and drought stress

PROTOPLASMA ◽

10.1007/s00709-015-0914-2 ◽

2015 ◽

Vol 254 (1) ◽

pp. 109-124 ◽

Cited By ~ 9

Author(s):

Manas Kumar Tripathy ◽

Budhi Sagar Tiwari ◽

Malireddy K. Reddy ◽

Renu Deswal ◽

Sudhir K. Sopory

Keyword(s):

Oryza Sativa ◽

Drought Stress ◽

Oryza Sativa L ◽

Ectopic Expression ◽

Seed Setting ◽

Rice Plants

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Responses of the Photosynthetic Electron Transport Reactions Stimulate the Oxidation of the Reaction Center Chlorophyll of Photosystem I, P700, under Drought and High Temperatures in Rice

International Journal of Molecular Sciences ◽

10.3390/ijms20092068 ◽

2019 ◽

Vol 20 (9) ◽

pp. 2068 ◽

Cited By ~ 10

Author(s):

Shinya Wada ◽

Daisuke Takagi ◽

Chikahiro Miyake ◽

Amane Makino ◽

Yuji Suzuki

Keyword(s):

Drought Stress ◽

Electron Transport ◽

Reaction Center ◽

High Temperatures ◽

Photosystem I ◽

Stress Responses ◽

Oryza Sativa L ◽

Photosynthetic Electron Transport ◽

Temperature Regimes ◽

Underlying Mechanisms

It is of interest how photosynthetic electron transport (PET) reactions respond to excess light energy caused by the combination of drought stress and high temperatures. Since such information is scarcely available for photosystem I (PSI), this question was explored in rice (Oryza sativa L.) plants subjected to drought stress, using culture solutions that contain poly(ethylene glycol) at different concentrations under two day/night temperature regimes. At 27/22 °C (day/night), drought stress led to the oxidation of the reaction center of the chlorophyll of PSI (P700), and also led to decreases in the quantum efficiencies of photosystem II (PSII) and PSI, and a reduction of the primary quinone electron acceptor of PSI. Such drought stress responses were wholly stimulated at 35/30 °C. These parameters were strongly correlated with each other and were minimally affected by temperature. These results indicate that the drought stress responses of the respective PET reactions are closely associated with each other in the oxidization of P700 and that such responses are stimulated at high temperatures. The underlying mechanisms of these phenomena were discussed. While P700 oxidation is thought to suppress reactive oxygen species (ROS) production, PSI photoinhibition was observed under severe stress conditions, implying that P700 oxidation is not sufficient for the protection of PSI under drought stress.

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ZmASR3 from the Maize ASR Gene Family Positively Regulates Drought Tolerance in Transgenic Arabidopsis

International Journal of Molecular Sciences ◽

10.3390/ijms20092278 ◽

2019 ◽

Vol 20 (9) ◽

pp. 2278 ◽

Cited By ~ 5

Author(s):

Yani Liang ◽

Yingli Jiang ◽

Ming Du ◽

Baoyan Li ◽

Long Chen ◽

...

Keyword(s):

Drought Stress ◽

Drought Tolerance ◽

Stress Responses ◽

Stomatal Closure ◽

Molecular Features ◽

Ros Accumulation ◽

Drought Conditions ◽

Relative Water ◽

Transgenic Lines ◽

Related Stress

Abscisic acid (ABA)-, stress-, and ripening-induced (ASR) proteins are reported to be involved in drought stress responses. However, the function of maize ASR genes in enhancing drought tolerance is not known. Here, nine maize ASR members were cloned, and the molecular features of these genes were analyzed. Phenotype results of overexpression of maize ZmASR3 gene in Arabidopsis showed lower malondialdehyde (MDA) levels and higher relative water content (RWC) and proline content than the wild type under drought conditions, demonstrating that ZmASR3 can improve drought tolerance. Further experiments showed that ZmASR3-overexpressing transgenic lines displayed increased stomatal closure and reduced reactive oxygen species (ROS) accumulation by increasing the enzyme activities of superoxide dismutase (SOD) and catalase (CAT) under drought conditions. Moreover, overexpression of ZmASR3 in Arabidopsis increased ABA content and reduced sensitivity to exogenous ABA in both the germination and post-germination stages. In addition, the ROS-related, stress-responsive, and ABA-dependent pathway genes were activated in transgenic lines under drought stress. Taken together, these results suggest that ZmASR3 acts as a positive regulator of drought tolerance in plants.

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A cytochrome P450, OsDSS1, is involved in growth and drought stress responses in rice (Oryza sativa L.)

Plant Molecular Biology ◽

10.1007/s11103-015-0310-5 ◽

2015 ◽

Vol 88 (1-2) ◽

pp. 85-99 ◽

Cited By ~ 43

Author(s):

Muluneh Tamiru ◽

Jerwin R. Undan ◽

Hiroki Takagi ◽

Akira Abe ◽

Kakoto Yoshida ◽

...

Keyword(s):

Cytochrome P450 ◽

Oryza Sativa ◽

Drought Stress ◽

Stress Responses ◽

Oryza Sativa L

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Jacalin domain-containing protein OsSalT interacts with OsDREB2A and OsNAC1 to impart drought stress tolerance in planta

10.1101/2020.04.19.049296 ◽

2020 ◽

Author(s):

Salman Sahid ◽

Chandan Roy ◽

Dibyendu Shee ◽

Riddhi Datta ◽

Soumitra Paul

Keyword(s):

Drought Stress ◽

Drought Tolerance ◽

Stress Tolerance ◽

Ectopic Expression ◽

Climatic Conditions ◽

Stress Factors ◽

Bimolecular Fluorescence Complementation ◽

In Planta ◽

Drought Stress Tolerance ◽

Transgenic Lines

AbstractWith the changing climatic conditions, drought has become one of the most threatening abiotic stress factors that adversely affect rice cultivation and productivity. Although the involvement of the jacalin domain-containing protein, OsSalT, has been reported in drought and salinity tolerance, its functional mechanism still remains largely unexplored. In this study, expression of the OsSalT gene was found to be positively correlated with the drought tolerance potential with its higher transcript abundance in the tolerant indica rice cultivar, Vandana and lower abundance in the susceptible cultivar, MTU1010. Moreover, the ectopic expression of OsSalT in tobacco imparted drought stress tolerance in the transgenic lines. The transgenic lines exhibited significantly improved growth and higher osmolyte accumulation over the wild-type (WT) plants together with the induction in the OsSalT expression under drought stress. Fascinatingly, the yeast two-hybrid and bimolecular fluorescence complementation (BiFC) analyses confirmed the interaction of OsSalT protein with two interesting transcription factors (TFs), OsNAC1 and OsDREB2A. In silico analysis further revealed that the OsSalT protein interacted with the regulatory domain of OsDREB2A and the C-terminal domain of OsNAC1 leading to their activation and induction of their downstream drought-responsive genes. Together, this study unravels a novel model for OsSalT-mediated regulation of drought tolerance in plants.

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