LncRNA TCONS_00021861 is functionally associated with drought tolerance in rice (Oryza sativa L.) via competing endogenous RNA regulation

Abstract Background Water deficit is an abiotic stress that retards plant growth and destabilizes crop production. Long non coding RNAs (lncRNAs) are a class of non-coding endogenous RNAs that participate in diverse cellular processes and stress responses in plants. lncRNAs could function as competing endogenous RNAs (ceRNA) and represent a novel layer of gene regulation. However, the regulatory mechanism of lncRNAs as ceRNA in drought stress response is yet unclear. Results In this study, we performed transcriptome-wide identification of drought-responsive lncRNAs in rice. Thereafter, we constructed a lncRNA-mediated ceRNA network by analyzing competing relationships between mRNAs and lncRNAs based on ceRNA hypothesis. A drought responsive ceRNA network with 40 lncRNAs, 23 miRNAs and 103 mRNAs was obtained. Network analysis revealed TCONS_00021861/miR528-3p/YUCCA7 regulatory axis as a hub involved in drought response. The miRNA-target expression and interaction were validated by RT-qPCR and RLM-5’RACE. TCONS_00021861 showed significant positive correlation (r = 0.7102) with YUCCA7 and negative correlation with miR528-3p (r = -0.7483). Overexpression of TCONS_00021861 attenuated the repression of miR528-3p on YUCCA7, leading to increased IAA (Indole-3-acetic acid) content and auxin overproduction phenotypes. Conclusions TCONS_00021861 could regulate YUCCA7 by sponging miR528-3p, which in turn activates IAA biosynthetic pathway and confer resistance to drought stress. Our findings provide a new perspective of the regulatory roles of lncRNAs as ceRNAs in drought resistance of rice.

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A comprehensive overview of miRNA targeting drought stress resistance in plants

Brazilian Journal of Biology ◽

10.1590/1519-6984.242708 ◽

2023 ◽

Vol 83 ◽

Author(s):

S. M. S. Shah ◽

F. Ullah

Keyword(s):

Drought Stress ◽

Stress Responses ◽

Crop Production ◽

Genetic Alterations ◽

Cereal Crops ◽

Transcriptional Level ◽

Gene Expressions ◽

Comprehensive Overview ◽

Defense Systems ◽

The Given

Abstract MicroRNAs (miRNAs) are essential nonprotein-coding genes. In a range of organisms, miRNAs has been reported to play an essential role in regulating gene expressions at post-transcriptional level. They participate in most of the stress responsive processes in plants. Drought is an ultimate abiotic stress that affects the crop production. Therefore understanding drought stress responses are essential to improve the production of agricultural crops. Throughout evolution, plants have developed their own defense systems to cope with the adversities of environmental stresses. Among defensive mechanisms include the regulations of gene expression by miRNAs. Drought stress regulates the expression of some of the functionally conserved miRNAs in different plants. The given properties of miRNAs provide an insight to genetic alterations and enhancing drought resistance in cereal crops. The current review gives a summary to regulatory mechanisms in plants as well as miRNAs response to drought stresses in cereal crops. Some possible approaches and guidelines for the exploitation of drought stress miRNA responses to improve cereal crops are also described.

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Overexpression of Rice OsS1Fa1 Gene Confers Drought Tolerance in Arabidopsis

Plants ◽

10.3390/plants10102181 ◽

2021 ◽

Vol 10 (10) ◽

pp. 2181

Author(s):

Sung-Il Kim ◽

Kyu Ho Lee ◽

Jun Soo Kwak ◽

Dae Hwan Kwon ◽

Jong Tae Song ◽

...

Keyword(s):

Drought Stress ◽

Stress Responses ◽

Oryza Sativa L ◽

Ectopic Expression ◽

Rice Seedlings ◽

Small Peptides ◽

Acid Protein ◽

Transgenic Lines ◽

Root Tissues ◽

Amino Acid Protein

Small peptides and proteins play critical regulatory roles in plant development and environmental stress responses; however, only a few of these molecules have been identified and characterized to date because of their poor annotation and other experimental challenges. Here, we present that rice (Oryza sativa L.) OsS1Fa1, a small 76-amino acid protein, confers drought stress tolerance in Arabidopsis thaliana. OsS1Fa1 was highly expressed in leaf, culm, and root tissues of rice seedlings during vegetative growth and was significantly induced under drought stress. OsS1Fa1 overexpression in Arabidopsis induced the expression of selected drought-responsive genes and enhanced the survival rate of transgenic lines under drought. The proteasome inhibitor MG132 protected the OsS1Fa1 protein from degradation. Together, our data indicate that the small protein OsS1Fa1 is induced by drought and is post-translationally regulated, and the ectopic expression of OsS1Fa1 protects plants from drought stress.

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Responses of the Photosynthetic Electron Transport Reactions Stimulate the Oxidation of the Reaction Center Chlorophyll of Photosystem I, P700, under Drought and High Temperatures in Rice

International Journal of Molecular Sciences ◽

10.3390/ijms20092068 ◽

2019 ◽

Vol 20 (9) ◽

pp. 2068 ◽

Cited By ~ 10

Author(s):

Shinya Wada ◽

Daisuke Takagi ◽

Chikahiro Miyake ◽

Amane Makino ◽

Yuji Suzuki

Keyword(s):

Drought Stress ◽

Electron Transport ◽

Reaction Center ◽

High Temperatures ◽

Photosystem I ◽

Stress Responses ◽

Oryza Sativa L ◽

Photosynthetic Electron Transport ◽

Temperature Regimes ◽

Underlying Mechanisms

It is of interest how photosynthetic electron transport (PET) reactions respond to excess light energy caused by the combination of drought stress and high temperatures. Since such information is scarcely available for photosystem I (PSI), this question was explored in rice (Oryza sativa L.) plants subjected to drought stress, using culture solutions that contain poly(ethylene glycol) at different concentrations under two day/night temperature regimes. At 27/22 °C (day/night), drought stress led to the oxidation of the reaction center of the chlorophyll of PSI (P700), and also led to decreases in the quantum efficiencies of photosystem II (PSII) and PSI, and a reduction of the primary quinone electron acceptor of PSI. Such drought stress responses were wholly stimulated at 35/30 °C. These parameters were strongly correlated with each other and were minimally affected by temperature. These results indicate that the drought stress responses of the respective PET reactions are closely associated with each other in the oxidization of P700 and that such responses are stimulated at high temperatures. The underlying mechanisms of these phenomena were discussed. While P700 oxidation is thought to suppress reactive oxygen species (ROS) production, PSI photoinhibition was observed under severe stress conditions, implying that P700 oxidation is not sufficient for the protection of PSI under drought stress.

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A cytochrome P450, OsDSS1, is involved in growth and drought stress responses in rice (Oryza sativa L.)

Plant Molecular Biology ◽

10.1007/s11103-015-0310-5 ◽

2015 ◽

Vol 88 (1-2) ◽

pp. 85-99 ◽

Cited By ~ 43

Author(s):

Muluneh Tamiru ◽

Jerwin R. Undan ◽

Hiroki Takagi ◽

Akira Abe ◽

Kakoto Yoshida ◽

...

Keyword(s):

Cytochrome P450 ◽

Oryza Sativa ◽

Drought Stress ◽

Stress Responses ◽

Oryza Sativa L

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A critical review on the improvement of drought stress tolerance in rice (Oryza sativa L.)

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha48412128 ◽

2020 ◽

Vol 48 (4) ◽

pp. 1756-1788

Author(s):

Adnan RASHEED ◽

Muhammad U. HASSAN ◽

Muhammad AAMER ◽

Maria BATOOL ◽

Sheng FANG ◽

...

Keyword(s):

Drought Stress ◽

Crop Production ◽

Genetic Manipulation ◽

Oryza Sativa L ◽

Molecular Techniques ◽

Plant Tolerance ◽

Rice Genotypes ◽

Available Information ◽

Global Strategies

Abiotic stresses are the primary threat to crop production across the globe. Drought stress is primary abiotic stress which is considerably limiting the global rice production and putting the food security at higher risks. Drought tolerance (DT) is a multigene trait which is influenced by various stages of development in rice plant. Tolerance as well as susceptibility of rice to drought stress is carried out by different drought-response genes and other components of stress. Plant researchers have used various methods such as, genetic manipulation and marker-assisted techniques for development of new rice cultivars with improved tolerance to drought stress. The aims of this review are to present recent advancements and illustrate current approaches to breed a robust drought-resistant rice genotypes by using classical breeding and advanced molecular techniques. We also shed light on all available information regarding the role of significant hormones in DT, QTL for drought-related traits, QTL for rice yield, global strategies for the improvement of DT in rice, DT genes, and selection supported by markers.

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Phosphate starvation response precedes drought response in field-grown plants

10.1101/2021.07.28.453724 ◽

2021 ◽

Author(s):

Yukari Nagatoshi ◽

Kenta Ikazaki ◽

Nobuyuki Mizuno ◽

Yasufumi Kobayashi ◽

Kenichiro Fujii ◽

...

Keyword(s):

Gene Expression ◽

Drought Stress ◽

Stress Responses ◽

Crop Production ◽

Phosphate Starvation ◽

Drought Response ◽

Molecular Response ◽

Starvation Response ◽

Phosphate Starvation Response ◽

Mild Drought

Drought severely damages crop production, even under conditions so mild that the leaves show no signs of wilting. As effective methods for analyzing the field drought response have not been established, it is unclear how field-grown plants respond to mild drought. We show that ridges are a useful experimental tool to mimic mild drought stress in the field. Mild drought reduces inorganic phosphate levels in the leaves to activate the phosphate starvation response (PSR) in field-grown soybean plants. PSR-related gene expression is mainly observed under drought conditions that are too mild to activate abscisic acid-mediated gene expression. Thus, our study provides insights into the molecular response to mild drought in field-grown plants and into the link between nutritional and drought stress responses in plants.

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Calcineurin B-like interacting protein kinase OsCIPK23 functions in pollination and drought stress responses in rice (Oryza sativa L.)

Journal of Genetics and Genomics ◽

10.1016/s1673-8527(08)60073-9 ◽

2008 ◽

Vol 35 (9) ◽

pp. 531-S2 ◽

Cited By ~ 60

Author(s):

Wenqiang Yang ◽

Zhaosheng Kong ◽

Edith Omo-Ikerodah ◽

Wenying Xu ◽

Qun Li ◽

...

Keyword(s):

Oryza Sativa ◽

Drought Stress ◽

Protein Kinase ◽

Stress Responses ◽

Oryza Sativa L ◽

Interacting Protein ◽

Calcineurin B

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SAPK2 contributes to rice yield by modulating nitrogen metabolic processes under drought stress

10.21203/rs.3.rs-19566/v1 ◽

2020 ◽

Author(s):

Dengji Lou ◽

Xiaoyan Yang

Keyword(s):

Drought Stress ◽

Osmotic Stress ◽

Seedling Growth ◽

Stress Responses ◽

Rice Yield ◽

Oryza Sativa L ◽

Rice Seedling ◽

Utilization Efficiency ◽

Influx Rate ◽

Rice Mutant

Abstract Background The sucrose non-fermenting 1-related kinases 2 (SnRK2s) play important roles in osmotic stress responses in A. thaliana and rice ( Oryza sativa L.). Osmotic stress/ABA–activated protein kinase 2 (SAPK2) is a member of SnRK2s subclass II in rice, but its function in rice yield under drought stress is unclear. Results Compared with wild-type (Oryza.Sativa L.spp.japonica, WT) plants, the sapk2 rice mutant lines were shorter and produced fewer grains per panicle, smaller grains and lower grain yield. Subsequent analysis suggested that SAPK2 considerably influences the nitrogen, phosphorus, and potassium contents of rice grains. The examination of rice seedling growth and development under nutrient-deprived conditions (−N, −K, and −P) proved that SAPK2 can significantly affect rice seedling growth and root development in hydroponic cultures lacking N and K. Moreover, the NO 3 − influx rate and nitrate concentration analysis indicated that SAPK2 promotes nitrate uptake and assimilation by regulating nitrate-related transporters. Conclusion These results suggest that SAPK2 could enhance grain production by regulating nitrogen utilization efficiency under drought stress. Our work provided insights to breeding drought tolerant rice with high nutrient uptake.

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