By-Products of the Black Soybean Sauce Manufacturing Process as Potential Antioxidant and Anti-Inflammatory Materials for Use as Functional Foods

To assess the potential of by-products of the black bean fermented soybean sauce manufacturing process as new functional food materials, we prepared black bean steamed liquid lyophilized product (BBSLP) and analysed its antioxidant effects in vitro. RAW264.7 macrophages were cultured and treated with BBSLP for 24 h, and 1 μg/mL lipopolysaccharide (LPS) was then used for another 24 h to induce inflammation. The cellular antioxidant capacity and inflammatory response were then analysed. Activation of nuclear factor kappa B (NF-κB) signaling in RAW264.7 macrophages was also analysed. Results showed BBSLP had 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium (ABTS+) radical-scavenging abilities and reducing power in vitro. The levels of both reactive oxygen species (ROS) and thiobarbituric acid reactive substances (TBARS) were reduced after RAW264.7 macrophages were treated with BBSLP after LPS induction. After RAW264.7 macrophage treatment with BBSLP and induction by LPS, the levels of inflammatory molecules, including nitric oxide (NO), prostaglandin E2 (PGE2), IL-1α, IL-6 and TNF-α, decreased. NF-κB signaling activity was inhibited by reductions in IκB phosphorylation and NF-κB DNA-binding activity after RAW264.7 macrophages were treated with BBSLP after LPS induction. In conclusion, BBSLP has antioxidant and anti-inflammatory capabilities and can be a supplement material for functional food.

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Antioxidant and Anti-inflammatory Activities of Cynaroside from Elsholtiza bodinieri

Natural Product Communications ◽

10.1177/1934578x1801301122 ◽

2018 ◽

Vol 13 (11) ◽

pp. 1934578X1801301 ◽

Cited By ~ 4

Author(s):

Ying Zou ◽

Min Zhang ◽

Tingrui Zhang ◽

Junwen Wu ◽

Jun Wang ◽

...

Keyword(s):

Radical Scavenging ◽

Reducing Power ◽

Total Content ◽

Preparative Hplc ◽

Dpph Radical ◽

Anti Inflammatory ◽

Flavonoid Fraction ◽

Reducing Power Assay

The flavonoid fraction was obtained from Elsholtiza bodinieri Vaniot (EBV) by ethanol-reflux and liquid-liquid extraction. The total content of flavonoid was 179.55 mg/g, and the purity was 64.6%. Then cynaroside with the purity of 94% was isolated from the fraction by preparative HPLC and characterized by the combined usage of HPLC, ESI-MS, and NMR. The antioxidant activity of cynaroside was determined using 2 complementary methods, namely, 2,2- diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and reducing power assay. The anti-inflammatory effect of cynaroside was investigated based on in-vitro and in-vivo experiment. The results showed that cynaroside from EBV scavenged DPPH radical and reduced Fe3+ to Fe2+ effectively, inhibited NO and ROS production in LPS-stimulated RAW264.7 cells and attenuated the inflammation in the mouse model significantly ( p < 0.01), which showed it to be a nutraceutical product in the food industry.

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Nauclea latifolia Sm. Leaf Extracts Extenuates Free Radicals, Inflammation, and Diabetes-Linked Enzymes

Oxidative Medicine and Cellular Longevity ◽

10.1155/2020/5612486 ◽

2020 ◽

Vol 2020 ◽

pp. 1-13 ◽

Cited By ~ 1

Author(s):

Franklyn Nonso Iheagwam ◽

Emmanuel Nsedu Israel ◽

Kazeem Oyindamola Kayode ◽

Opeyemi Christianah DeCampos ◽

Olubanke Olujoke Ogunlana ◽

...

Keyword(s):

Competitive Inhibition ◽

Radical Scavenging ◽

Reducing Power ◽

Peroxide Radical ◽

Hypotonic Solution ◽

Leaf Extracts ◽

Inhibitory Activities ◽

Anti Inflammatory ◽

Nauclea Latifolia

This study was carried out to assess the in vitro antioxidant, anti-inflammatory and antidiabetic effects of Nauclea latifolia (Sm.) leaf extracts. Ethanolic (NLE) and aqueous (NLA) extract of N. latifolia leaves were prepared and assessed for their anti-inflammatory activity, antioxidant potential, α-amylase and α-glucosidase inhibitory activities, and the mechanism of enzyme inhibition in vitro using standard established methods. From the results, phytochemicals such as flavonoids, phenolics, glycosides, and tannins were detected in both extracts of N. latifolia with NLE having a significantly (p<0.05) higher phytochemical content. NLE displayed significantly (p<0.05) better total antioxidant capacity, reducing power, 2,2-diphenyl-2-picrylhydrazyl, and hydrogen peroxide radical scavenging activities. For anti-inflammatory activities, 70.54±2.45% albumin denaturation inhibition was observed for NLE while 68.05±1.03% was recorded for NLA. Likewise, 16.07±1.60 and 14.08±1.76% were obtained against hypotonic solution and heat-induced erythrocyte haemolysis, respectively, for NLE while 20.59±4.60 and 24.07±1.60% were respective NLA values. NLE (IC50: 4.20±0.18 and 1.19±0.11 mg/mL) and NLA (IC50: 11.21±0.35 and 2.64±0.48 mg/mL) α-glucosidase and α-amylase inhibitory activities were dose-dependent with uncompetitive and competitive inhibition elicited, respectively, by the extracts. A significant positive association (p<0.01 and 0.05) was identified between antioxidant activity and carbohydrate-metabolising enzyme inhibitory activity. The obtained result suggests N. latifolia leaf could serve as an alternative candidate for managing diabetes mellitus due to its antioxidant and anti-inflammatory association with diabetes-linked enzymes.

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ENHANCEMENT OF FUNCTIONAL PROPERTIES AND BIOLOGICAL ACTIVITY IN BARLEY AND WHEAT GRAINS BY GERMINATION

Scientific Journal of Applied Sciences of Sabratha University ◽

10.47891/sabujas.v2i3.20-41 ◽

2019 ◽

Vol 2 (3) ◽

pp. 20-41

Author(s):

Naji Elhadi Aborus ◽

Jasna Čanadanović Brunet ◽

Vesna Šaponjac ◽

Jelena Vulić

Keyword(s):

Functional Food ◽

Antioxidant Activities ◽

Diclofenac Sodium ◽

Reducing Power ◽

Inhibitory Effect ◽

Strong Positive Correlation ◽

Freeze Dried ◽

Total Flavonoids Content ◽

Anti Inflammatory

In recent years, sprouted grains have become very popular and widely accepted as a functional food because of their nutritious and health benefits. So, this study was performed to evaluate the phytochemical composition, and in vitro antioxidant capacity, reducing power, antihyperglycemic and anti-inflammatory activities, of seven-day old cereal sprouts (CS): Cultivars, barley NS565 (BSNS), barley Golozrni (BSG), wheat Spelta (WSSPE), wheat Simonida (WSSIM). Phenolic compounds were the most dominant bioactives in all CS. BSNS expressed significantly higher (p≤ 0.05) content of total phenols, chlorophyll and carotenoids. The total flavonoids content (TFC) in CS showed that barley sprouts (BSNS, BSG) had the higher value (P≤ 0.05) than wheat sprouts (WSSPE, WSSIM) respectively. The freeze-dried sprouts powders (FDSP) extracts were screened for possible antioxidant capacities using DPPH, ABTS, and reducing power (Rp) assays. The results indicated that the BSNS possessed higher antioxidant capacities in DPPH and ABTS assays, and reducing power (IC50DPPH = 0.54 mg/ml; IC50ABTS = 0.79 mg/ml; IC0.5RP = 9.35 mg/ml) respectively. The inhibitory effect of FDSP extracts on α-glucosidase activity was investigated. The BSNS extracts exhibited higher inhibitory activity (IC50AHgA = 1.43 mg/ml) against α-glucosidase (p≤ 0.05). The anti-inflammatory activity (Denaturation of protein in vitro) showed significantly different between the FDSP, and Diclofenac sodium (DS). The IC50AIA of DS and BSNS was 0.79 and 1.86 (mg/ml) respectively. There was a strong positive correlation between TPC and antioxidant activities and reducing power, and also between TFC and anti-inflammatory activity.

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Mint Oils: In Vitro Ability to Perform Anti-Inflammatory, Antioxidant, and Antimicrobial Activities and to Enhance Intestinal Barrier Integrity

Antioxidants ◽

10.3390/antiox10071004 ◽

2021 ◽

Vol 10 (7) ◽

pp. 1004

Author(s):

Monika Hejna ◽

Lauren Kovanda ◽

Luciana Rossi ◽

Yanhong Liu

Keyword(s):

Antioxidant Activities ◽

Biological Activities ◽

Radical Scavenging ◽

Reducing Power ◽

Feed Additives ◽

Enterotoxigenic Escherichia Coli ◽

Barrier Integrity ◽

Tnf Α ◽

Anti Inflammatory

The objectives of the study were to test the biological activities of peppermint and spearmint oils via (i) measuring in vitro anti-inflammatory effects with porcine alveolar macrophages (PAMs), (ii) determining the barrier integrity of IPEC-J2 by analyzing transepithelial electrical resistance (TEER), (iii) testing their antioxidant activities, and (iv) investigating the antimicrobial activity against enterotoxigenic Escherichia coli (ETEC) F18+. Briefly, (i) macrophages were seeded at 106 cells/mL and treated (24 h) with mint oils and lipopolysaccharide (LPS). The treatments were 2 (0 or 1 μg/mL of LPS) × 5 (0, 25, 50, 100, 200 µg/mL of mint oils). The supernatants were collected for TNF-α and IL-1β measurement by ELISA; (ii) IPEC-J2 cells were seeded at 5 × 105 cells/mL and treated with mint oils (0, 25, 50, 100, and 200 μg/mL). TEER (Ωcm2) was measured at 0, 24, 48, and 72 h; (iii) the antioxidant activity was assessed (0, 1, 50, 100, 200, 500, and 600 mg/mL) using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and reducing power assays; (iv) overnight-grown ETEC F18+ were quantified (CFU/mL) after supplementing with peppermint and spearmint oils (0, 1.44, 2.87, 5.75, 11.50, and 23.00 mg/mL). All data were analyzed using the MIXED procedure. Both mint oils significantly inhibited (p < 0.05) IL-1β and TNF-α secretion from LPS-stimulated PAMs. Mint oil treatments did not affect TEER in IPEC-J2. Spearmint and peppermint oils exhibited (p < 0.05) strong antioxidant activities in DPPH and reducing power assays. Both mint oils also dose-dependently inhibited (p < 0.05) the growth of ETEC F18+ in vitro. The results of the study indicated that both mint oils are great candidate feed additives due to their in vitro anti-inflammatory, antioxidant, and antimicrobial effects. Further research is needed to evaluate their efficacy in vivo.

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Antioxidant Potential of Chestnut (Castanea sativa L.) and Almond (Prunus dulcis L.) By-products

Food Science and Technology International ◽

10.1177/1082013209353983 ◽

2010 ◽

Vol 16 (3) ◽

pp. 209-216 ◽

Cited By ~ 25

Author(s):

J.C.M. Barreira ◽

I.C.F.R. Ferreira ◽

M.B.P.P. Oliveira ◽

J.A. Pereira

Keyword(s):

Lipid Peroxidation ◽

Bioactive Compounds ◽

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Reducing Power ◽

Castanea Sativa ◽

Thiobarbituric Acid ◽

Waste Products ◽

By Products

The antioxidant properties of almond green husks (Cvs. Duro Italiano, Ferraduel, Ferranhês, Ferrastar and Orelha de Mula), chestnut skins and chestnut leaves (Cvs. Aveleira, Boa Ventura, Judia and Longal) were evaluated through several chemical and biochemical assays in order to provide a novel strategy to stimulate the application of waste products as new suppliers of useful bioactive compounds, namely antioxidants. All the assayed by-products revealed good antioxidant properties, with very low EC50 values (lower than 380 μg/mL), particularly for lipid peroxidation inhibition (lower than 140 μg/mL). The total phenols and flavonoids contents were also determined. The correlation between these bioactive compounds and DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity, reducing power, inhibition of β-carotene bleaching and inhibition of lipid peroxidation in pig brain tissue through formation of thiobarbituric acid reactive substances, was also obtained. Although, all the assayed by-products proved to have a high potential of application in new antioxidants formulations, chestnut skins and leaves demonstrated better results.

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In vitro antioxidant and anti-inflammatory activities of the chloroform extract of Combretum punctatum var squamosum

Science Vision ◽

10.33493/scivis.19.03.04 ◽

2019 ◽

Vol 19 (3) ◽

pp. 100-108

Author(s):

K. Thanzami ◽

B.B. Kakoti ◽

C. Lalremruati

Keyword(s):

Lipid Peroxidation ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Reducing Power ◽

Chloroform Extract ◽

In Vitro Models ◽

Percent Inhibition ◽

Anti Inflammatory

The objective of this study was to investigate the antioxidant and anti-inflammatory activities of the chloroform extract of Combretum punctatum var squamosum by in vitro models. Antioxidant activity was determined by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, reducing power and lipid peroxidation assays. The phenolic and flavonoid contents of the extract were also assessed. The extract at different concentrations showed significant percent inhibition of DPPH and lipid peroxidation and high reducing power when compared with the standard. The extract also showed a significantly high content of phenolics and flavonoids. Anti-inflammatory activity was studied by inhibition of albumin denaturation and human red blood cell (HRBC) membrane stabilization methods. The extract at different concentrations showed significant percent inhibition of albumin denaturation and percent inhibition of haemolysis when compared with the standard. Thus, the result indicates that the chloroform extract exhibited significant potential on anti-inflammatory and antioxidant activities.

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ANTIOXIDANT AND ANTI-INFLAMMATORY PROPERTIES OF PTEROSPERMUM RUBIGINOSUM HEYNE EX WIGHT AND ARN AND PTEROSPERMUM RETICULATUM WIGHT AND ARN (STERCULIACEAE): AN IN VITRO COMPARATIVE STUDY

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2019.v12i2.28137 ◽

2019 ◽

pp. 272-275 ◽

Cited By ~ 1

Author(s):

JENSON JACOB ◽

SREEJITH K

Keyword(s):

Nitric Oxide ◽

Folk Medicine ◽

Radical Scavenging ◽

Reducing Power ◽

Protease Inhibition ◽

Nitric Oxide Radical ◽

The Family ◽

Anti Inflammatory ◽

Reducing Power Assay

Objectives: Plants from the family Sterculiaceae are used as folk medicine for treating various diseases in India. This study aims to determine the antioxidant and anti-inflammatory properties of Pterospermum rubiginosum and Pterospermum reticulatum of the family Sterculiaceae. The barks of P. rubiginosum and P. reticulatum are used in traditional medicine especially in the treatment of wounds, sprains, bone fracture, etc. This study, we compare the antioxidant and anti-inflammatory potentials of the stem bark of these two plants. Methods: The free radical scavenging assays such as 2,2–diphenyl,1–picrylhydrazyl (DPPH), 2,2’–azino-bis(3-ethylbenzothiozoline-6-sulfonic acid) (ABTS), hydroxyl radical, nitric oxide radical, phosphormolybdenum assay, and reducing power assay are used for the measurement of antioxidant potentials. The in vitro anti-inflammatory activities of the extracts are evaluated by means of lipoxygenase (LOX) and protease inhibition. Results: Both P. rubiginosum and P. reticulatum scavenge DPPH (70.10% and 91.02%), ABTS (94.48 and 98.19%), hydroxy (76.02 and 87.67%), and nitric oxide (87.02 and 80.84%) radicals. Phosphomolybdenum assay and reducing power assay, used for the measurement of antioxidant potentials also showed good results. Regarding the anti-inflammatory potential, the methanolic extract of the plants shows anti-protease activity (51.29 and 64.93%) and anti-LOX activity (56%) while P. rubiginosum does not exhibit anti-LOX activity. Conclusion: The above results demonstrate that the plants P. rubiginosum and P. reticulatum are rich source of antioxidant and anti-inflammatory compounds and it is the first report on theantioxidant and anti-inflammatory properties of the barks of these plants.

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Antioxidant, Anti-Inflammatory, and Postulated Cytotoxic Activity of Phenolic and Anthocyanin-Rich Fractions from Polana Raspberry (Rubus idaeus L.) Fruit and Juice—In Vitro Study

Molecules ◽

10.3390/molecules23071812 ◽

2018 ◽

Vol 23 (7) ◽

pp. 1812 ◽

Cited By ~ 17

Author(s):

Urszula Szymanowska ◽

Barbara Baraniak ◽

Anna Bogucka-Kocka

Keyword(s):

Cytotoxic Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Leukemia Cell ◽

Reducing Power ◽

Rubus Idaeus ◽

Human Leukemia ◽

Cox 2 ◽

Anti Inflammatory

In this study, the antioxidative and anti-inflammatory potential of crude extracts (CE), anthocyanin-rich fractions (ARF), and phenolic fractions (PF) from raspberry (R) and raspberry juice (J) were evaluated. The antioxidant properties were evaluated with three complementary assays: DPPH radical scavenging activity, chelating Fe(II) power, and ferric reducing power. The highest antioxidant activity was determined for the crude extract from raspberry pulp (RCE) in the case of all methods used. The anti-inflammatory activity was demonstrated by inhibitory effect on lipoxygenase (LOX) and cyclooxygenase-2 (COX-2) activity in vitro. The highest efficiency in inhibiting the activity of both enzymes was exhibited by RCE, 0.79 and 0.59 mg FW/mL, respectively. In turn, JARF had the lowest ability to inhibit LOX (EC50 = 4.5 mg FW/mL) and JPF caused the lowest COX-2 inhibition (1.75 mg FW/mL). Additionally, we have performed a pilot study of in vitro cytotoxic activity using two human leukemia cell lines: J45 and HL60. All examined extracts inhibited the viability of J45 cells more effectively than HL60. The highest cytotoxic effect was observed in the J45.01 cell line after exposure to RCE (EC50 = 0.0375 mg FW/mL).

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In-vitro Antioxidant and anti-inflammatory activities valorisation of tannin crude extract of Helianthemum helianthemoïdes (Desf.) Grosser

Journal of Drug Delivery and Therapeutics ◽

10.22270/jddt.v10i1-s.3884 ◽

2020 ◽

Vol 10 (1-s) ◽

pp. 135-139

Author(s):

Nouioua Wafa ◽

Gaamoune Sofiane

Keyword(s):

Crude Extract ◽

Radical Scavenging ◽

Reducing Power ◽

Dpph Radical ◽

Stabilization Method ◽

Membrane Stabilization ◽

Inflammatory Agent ◽

Anti Inflammatory ◽

Dpph Radical Scavenging

Plants have always been used by humans to relieve and cure many diseases unfortunately the majority of them still unknown. The aims of this study were to evaluate the antioxidant and anti-inflammatory properties of tannin crude extract of Helianthemum helianthemoïdes (Desf.) Grosser. The antioxidant activity was performed by DPPH radical scavenging method and the reducing power essay; however, the anti-inflammatory activity was tested with the Human Red Blood Cell (HRBC) membrane stabilization method. The obtained results indicated high antioxidant potential and a perfect anti-inflammatory agent. Keywords: Helianthemum helianthemoïdes; DPPH; reducing power; HRBC

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Comparative screening of in-vitro free radical scavenging, anti-inflammatory and anti-haemolytic activities from non-polar solvent extracts of Pterocarpus marsupium

International Journal of Phytomedicine ◽

10.5138/09750185.2021 ◽

2017 ◽

Vol 9 (3) ◽

pp. 399

Author(s):

Aruna LH ◽

Sanjeevkumar CB ◽

Amarvani PK ◽

Ramesh L Londonkar

Keyword(s):

Free Radical ◽

Petroleum Ether ◽

Protein Denaturation ◽

Petroleum Ether Extract ◽

Radical Scavenging ◽

Reducing Power ◽

Free Radical Scavenging ◽

Pterocarpus Marsupium ◽

Anti Inflammatory

Pterocarpus marsupium is widely used as ‘Rasayana’ in ayurvedic system for curing several medical ailments. In view of this, petroleum ether and chloroform extracts of Pterocarpus marsupium bark was scrutinized to unfold free radical scavenging by measuring their capability for scavenging DPPH radical, ABTS, phosphomolybdenum assay as well as reducing power capacity assessment and anti- inflammatory activity by inhibition of protein denaturation. Additionally, RBC’S were also used to explore the potentiality of Pterocarpus marsupium different extracts to shield RBC in oxidative stress condition. Chloroform extract showed an effective pharmacological activity in all assays when compared with petroleum ether extract and their respective standards. This reveals the use of Pterocarpus marsupium as a potent source of antioxidant, anti-inflammatory, anti-haemolytic agent and would provide an ample opportunity for further investigation. Isolation of active compounds from this plant responsible for producing such bioactivities is under process.

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