VARIETAL FEATURES OF TUBERS OF JERUSALEM ARTICHOKE, GROWING IN THE KRASNODAR REGION AND THE Adygea Republic

Author(s):  
Н.Т. Шамкова ◽  
В.Ю. Токарев ◽  
А.В. Добровольская ◽  
А.З. Тодорова ◽  
А.И. ВЕРЕЩАГИНА

Определен биотехнологический потенциал топинамбура сортов Новость ВИР, Скороспелка, Интерес, произрастающего в Краснодарском крае и Республике Адыгея, урожаев 2011 2018 гг. Установлено, что клубни топинамбура сорта Интерес превосходят сорта Новость ВИР и Скороспелка по содержанию, : общих сахаров на 22,41 и 14,34 инулина 41,82 и 32,99 пектиновых веществ 22,39 и 49,75 аскорбиновой кислоты 35,27 и 39,29 хлорогеновой кислоты 3,66 и 18,29 оротовой кислоты 88,89 и 84,89 соответственно. Сорт Интерес в сравнении с сортами Новость ВИР и Скороспелка содержит на 245,24 и 243,33 меньше никотиновой кислоты и на 100,0 и 108,33 кофейной кислоты соответственно. Содержание гистидина в клубнях топинамбура сортов Интерес, Новость ВИР и Скороспелка составляет 132,36 44,83 и 88,21 мг/кг аргинина 2017,2 958,56 и 460,44 мг/кг соответственно. В комплексе с аскорбиновой кислотой органические кислоты клубней топинамбура обладают выраженными антиоксидантными свойствами. Установлено, что в результате бланширования антиоксидантная активность топинамбура сорта Интерес возросла на 15,6. Таким образом, сорт Интерес наиболее перспективен для переработки в условиях предприятий общественного питания. The biotechnological potential of Jerusalem artichoke varieties Novost VIR, Skorospelka, Interes growing in the Krasnodar region and the Adygea Republic, harvest 2011 2018 has been determined. It was found that Jerusalem artichoke tubers of the Interes variety are superior to Novost VIR and Skorospelka varieties in content, : total sugars by 22,41 and 14,34 inulin 41,82 and 32,99 pectin substances 22,39 and 49,75 ascorbic acid 35,27 and 39,29 chlorogenic acid 3,66 and 18,29 orotic acid 88,89 and 84,89, respectively. Variety Interes in comparison with Novost VIR and Skorospelka varieties contains 245,24 and 243,33 less nicotinic acid and 100,00 and 108,33 coffee acid, respectively. The content of histidine in the tubers of Jerusalem artichoke varieties of Interes, Novost VIR, Skorospelka is 132,36 44,83 and 88,21 mg/kg arginine 2017,2 958,56 and 460,44 mg/kg, respectively. In a complex with ascorbic acid, organic acids of Jerusalem artichoke tubers have pronounced antioxidant properties. It was found that as a result of blanching, the antioxidant activity of Jerusalem artichoke of the Interes variety increases by 15,6. Thus, the Interes variety is most promising for processing in public catering facilities.

Author(s):  
В.Ю. Токарев ◽  
Н.Т. Шамкова ◽  
А.В. Добровольская ◽  
А.З. Тодорова ◽  
А.И. ВЕРЕЩАГИНА

Исследованы изменения биохимических свойств клубней топинамбура (КТ) сортов Скороспелка (СК), Интерес (ИНТ), Новость ВИР (НВИР) урожаев 2011 2018 гг., выращиваемого в Краснодарском крае и Республике Адыгея, при хранении до поступления и в условиях предприятий общественного питания. Установлено, что при хранении на складах с искусственным охлаждением (склады с ИО) потери инулина составили, , сорт: ИНТ 10,4 НВИР 10,28 СК 22,01. При хранении в регулируемой газовой среде (РГС) потери инулина составили, , сорт: ИНТ 2,8 НВИР 5,91 СК 4,25 при хранении в грунте 9,4 9,59 и 7,92 соответственно. При хранении на складах с ИО увеличилось содержание пектиновых веществ, , сорт: ИНТ на 12,6 НВИР на 0,31 СК на 95,59 в РГС 2,54 1,71 и 2,55 соответственно при хранении в грунте 7,15 6,99 и 3,94 соответственно. При хранении на складах с ИО содержание хлорогеновой и кофейной кислот увеличилось, , сорт: ИНТ на 253,6 и 10852,7 НВИР на 83,5 и 997,9 СК на 361,0 и 661,3 соответственно в РГС 58,54 и 525,0 51,9 и 122,2 64,2 и 180 соответственно при хранении в грунте 82,9 и 983,3 77,2 и 191,67 79,1 и 246,67 соответственно. При хранении на складах с ИО уменьшилось содержание аскорбиновой кислоты, , сорт: ИНТ на 62,9 НВИР на 47,59 СК на 41,18 в РГС 28,97 30,96 и 34,04 соответственно в грунте 34,87 34,96 и 35,0 соответственно. Исследовано изменение структурномеханических свойств, естественной убыли клубней при различных способах хранения. Определено, что после 7 сут хранения в кладовой овощей снижается механическая прочность КТ, сорт: ИНТ на 26,8, НВИР на 30,0, СК на 31,25 соответственно после 14 сут хранения на 49,3 52,5 и 55,62 соответственно после 90 сут хранения в холодильной камере на 31,87 38,31 и 39,37 соответственно после 180 сут хранения в морозильной камере и размораживания на воздухе на 80,62 83,12 и 86,8 соответственно. Среднеотраслевая норма рентабельности продажи КТ 40. Investigated changes of biochemical properties of Jerusalem artichoke tubers varieties Skorospelka (SK), Interes (INT), Novost VIR (NVIR) harvests 2011 2018 grown in the Krasnodar region and the Adygea Republic at storage before receipt and in the conditions of public catering establishments. It was found that when stored in warehouses with artificial cooling (warehouses with AC), the loss of inulin was, , variety: INT 10,4 NVIR 10,28 SK 22,01. When stored in a controlled gas environment (CGE), the loss of inulin was, , variety: INT 2,8 NVIR 5,91 SK 4,25 when stored in soil 9,4 9,59 and 7,92, respectively. When stored in warehouses with AC, the content of pectin substances increased, , variety: INT by 12,6 NVIR at 0,31 SK at 95,59 in the CGE 2,54 1,71 and 2,55, respectively when stored in soil 7,15 6,99 and 3,94, respectively. During storage in the warehouses with AC with the contents of chlorogenic and caffeic acids increased, , variety: INT on 253,6 and 10852,7 NVIR by 83,5 and 997,9 SK on 361,0 and 661,3, respectively in CGE 58,54 and 525,0 51,9 and of 122,2 64,2 and 180, respectively in storage in the soil 82,9 and 983,3 77,2 and 191,67 79,1 and 246,67, respectively. When stored in warehouses with AC, the content of ascorbic acid decreased, , variety: INT by 62,9 NVIR at 47,59 SK at 41,18 in the CGE 28,97 30,96 and 34,04, respectively in the ground 34,87 34,96 and 35,0, respectively. The change in the structural and mechanical properties, the natural loss of tubers under various storage methods was investigated. It was determined that after 7 days of storage in the pantry of vegetables, the mechanical strength of Jerusalem artichoke tubers decreases, variety: INT by 26,8, NVIR by 30,0, SK by 31,25, respectively after 14 days of storage by 49,3 52,5 and 55,62, respectively after 90 days of storage in the refrigerator by 31,87 38,31 and 39,37, respectively after 180 days of storage in the freezer and defrosting in air by 80,62 83,12 and 86,8, respectively. The industry average rate of return on sales of Jerusalem artichoke tubers is 40.


2018 ◽  
Vol 51 (4) ◽  
pp. 164
Author(s):  
K. Khairiah ◽  
Irham Taufiqurrahman ◽  
Deby Kania Tri Putri

Background: Binjai (Mangifera caesia) is a herb derived from South Kalimantan possessing antioxidant properties which promote wound healing inhibiting oxidation radicals. The natural antioxidants present in binjai leaves can be extracted by fractionation. Purpose: This study aimed to analyze the antioxidant activity of ethyl acetate fraction in 96% ethanol extract of binjai leaf. Methods: The study constituted a pure experimental study incorporating a post-test design with only random sampling technique consisting of two groups, namely; an ethyl acetate fraction as the treatment group and ascorbic acid as the positive control group. The leaves were treated in accordance with the soxhlet method and subsequently fractionated to extract ethyl acetate fraction. This was used to measure antioxidant activity with DPPH radical damping method using a UV-Vis spectrophotometer. A linear regression calculation was performed with a standard curve to quantify the IC50 value, before the ethyl acetate fraction underwent a qualitative test of secondary metabolite. Results: An independent t-test indicated significant differences between groups, an average value of IC50 in ascorbic acid of 13.812 ppm with 0.996 linearity and a fraction of ethyl acetate 38.526 ppm with a linearity of 0.999. In contrast, at this linearity value ascorbic acid and ethyl fraction acetate demonstrate a very high linear connection between concentration and inhibition. A secondary metabolite test conducted on the ethyl acetate fraction produced positive results for flavonoid, tannins, and phenol. Conclusion: Based on the IC50 parameters, the fraction of ethyl acetate in 96% ethanol extract of binjai leaf produces very strong antioxidant activity in the content of the compounds in the fraction, namely: flavonoid, tannins and phenol.


Author(s):  
M. Suleman Stephen ◽  
E. A. Adelakun ◽  
J. H. Kanus ◽  
Meshack M. Gideon

The presence of natural antioxidant in plants is well known. Plant phenolics constitute one of the major groups of components that act as antioxidant and free radical terminator. Hence, this study focused on investigating the antioxidant activity of Celery plant (Apium graveolens L). The fresh leaves were collected, crushed and extracted with ethanol and acetone by maceration. The radical scavenging properties of the extracts were determined by measuring changes in absorbance of DPPH radical at a wave lenght of 517 nm by UV and ascorbic acid is used as the standard. It showed that the crude ethanolic extract has higher antioxidant activity compared to ascorbic acid and acetone extract with less scavenging activity. The values were (IC50 114.6 µg/mL) for ascorbic acid, (IC50 112 µg/mL) for the crude ethanolic extract and (IC50172 µg/mL) for crude acetone extract. The result shows that Celery plant grown in Jos possess good antioxidant properties which may be linked to the presence of phenolics and flavonoids in the plant, which justifies its use as a medicinal plant. This can be further investigated for the isolation and identification of active compounds of medicinal utilities.


2021 ◽  
Vol 913 (1) ◽  
pp. 012093
Author(s):  
U Fitrotin ◽  
N Hilmiati ◽  
Mardiana ◽  
Y Triguna ◽  
A Surahman ◽  
...  

Abstract Preparation process for meniran (Phillantus urinaria) functional drink (MFD) influences its antioxidant activity. This research aims to understand the phenolic content, DPPH Radical Scavenging Activity (RSA), and LDL oxidation of MFD through various preparation processes. Those preparation processes included soaking fresh meniran (SFM), boiling fresh meniran for 5 minutes (BFM5’), boiling fresh meniran for 10 minutes (BFM10’), and soaking dried meniran (DM). The phenolic content was determined with Folin–Ciocalteu, antioxidant activity was assessed using DPPH and TBARS assay with LDL as the oxidation substrate. An antioxidant references in this research used ascorbic acid. The phenolic content in methods of SFM, BFM5’, BFM10’ and DM were 122±0.022, 182±0.043, 192 ±0.03, and 117 ±0.019 mg GAE/g of meniran respectively. Meanwhile, the DPPH RSA of SFM, BFM5’, BFM10’ and DM accounted for 82.18±0.35, 86.19±0.53, 86.75±0.64 and 69.96% respectively. As comparison, the DPPH RSA of ascorbic acid 50 ppm is 75.65±0.82%. At the same time the optimum inhibition of TBARS formation from BFM5’ and BFM10’ methods were 45.83 % and 48.66%, with MDA concentration in human LDL accounted for 38.30±2.39 and 36.30±1.82 nmol MDA/mg protein, respectively. As comparison, MDA concentration in human LDL added with ascorbic acid 25 ppm accounted for 41.35±2.41 nmol MDA/mg protein. In contrast, the control human LDL was 70.70±2.35 nmol MDA/mg protein. This study concludes that the BFM5’ and BFM10’ methods showed the highest antioxidant properties compared to other methods. All methods showed that MFD extract in concentration more than 25 ppm increased the concentration of MDA in human LDL. Therefore, to produce meniran functional drink in optimum antioxidant properties is best by using BFM5’ and BFM10’ preparation methods in meniran concentration of not more than 25 ppm.


Author(s):  
Sergey S. Kletikov ◽  
Mariya V. Tesakova ◽  
Vladimir I. Parfenyuk ◽  
Vladislav R. Kulagin

The antioxidant activity of 5,10,15,20-tetrakis(4-hydroxyphenyl)porphyrin was established by a test reaction of interaction with free-radical 1,1-diphenyl-2-picrylhydrazyl (DPPH). A cyclic voltammetry method was used to research the interaction of porphyrin and DPPH. According to the results of the completed research, it was established that H2T(4-OHPh) P has the highest antioxidant activity among all the porphyrins compounds studied by us. The results of the electrochemical experiment served as a basis for testing antioxidant qualities of porphyrin on living creatures – chickens of the “Moscow-black” breed. Chickens were divided into 5 groups: the control group was given the basic ration, the first — the basic ration + 1% ascorbic acid solution, the second — the basic ration + an aqueous solution of Twin-80, the third — the basic ration + an aqueous solution of Twin-80 with porphyrin (10–3 mol/l), the fourth - basic ration + aqueous solution of Twin-80 with porphyrin (10-4 mol/l). In the course of the experiment, the chickens were orally administered the 2 ml solutions studied once a day in for 14 days. Wherein no disturbance of physiological condition of the chickens was noticed, as well as any considerable change of body mass or causes of death. The introduction solution Twin-80 does not impair the blood indexes. In all four experimental groups, a decrease in the concentration of malonic dialdehyde (MDA) was observed compared to the control group: the best result - a decrease in MDA by 56% is observed in the fourth group. In the three groups an increase in serum level of ceruloplasmin was observed. The greatest increase in the concentration of CP also occurs in the fourth group of chickens. With the introduction of ascorbic acid a decrease in CP relative to the control group is not observed. How it was established the 5,10,15,20-tetrakis(4-hydroxyphenyl)porphyrin with concentrations of 10-4 mole/l decline the amount of malonic dialdehyde and increases the amount of ceruloplasmin in blood, which leads to lowering the activity of oxidizing processes in a living organism and also empowers the protein synthesizing function of the liver.


Author(s):  
A. A. Ashinova

At the present stage, the tasks of state policy is to preserve the functions of life support for the stable development of society, improving the health of the population, ensuring environmental safety of the country. Antioxidant activity is manifested in the ability of substances to delay the radical oxidation of organic and high-molecular compounds, and thereby reduce the yield of alcohols, hydroperoxides, fatty acids, aldehydes and ketones. Pectin substances are contained in all plants and some algae and are able to exhibit antioxidant activity. The article presents the study of antioxidant activity of pectin-containing solutions. To determine the antioxidant activity, aqueous solutions of pectin substances of Apple, citrus, beet pectin, their combinations with a concentration of 1% were prepared. According to the obtained data, the value of antioxidants for aqueous solutions of pectin substances and their combinations was calculated. According to the diagram of antioxidant activity of aqueous solutions of pectin substances and their combinations. An increase in antioxidant activity in the combination of Apple and citrus pectins with beet, which is explained by the influence of beet pectin on the properties of substances combined with it. Apple pectin does not increase antioxidant activity in combination with citrus pectin, due to its chemical and physiological form. The ability of pectins to show antioxidant activity depending on the combination of pectins is proved. It was found that the most antioxidant activity has an aqueous solution of beet pectin. Moreover, there is an increase in antioxidant activity when combining Apple and citrus pectins with beet, which is explained by the predominant effect of beet pectin on this indicator.


Plants ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 482 ◽  
Author(s):  
Ahmed M. Abd-ElGawad ◽  
Abdelsamed I. Elshamy ◽  
Saud L. Al-Rowaily ◽  
Yasser A. El-Amier

The variation in habitat has a direct effect on the plants and as a consequence, changes their content of the bioactive constituents and biological activities. The present study aimed to explore the variation in the essential oils (EOs) and phenolics of Heliotropium curassavicum collected from the coastal and inland habitats. Additionally, we determined their antioxidant and allelopathic activity against the weed, Chenopodium murale. Fifty-six compounds were identified as overall from EOs, from which 25 components were identified from the coastal sample, and 52 from the inland one. Sesquiterpenes were the main class in both samples (81.67% and 79.28%), while mono (3.99% and 7.21%) and diterpenes (2.9% and 1.77%) represented minors, respectively. Hexahydrofarnesyl acetone, (-)-caryophyllene oxide, farnesyl acetone, humulene oxide, farnesyl acetone C, and nerolidol epoxy acetate were identified as major compounds. The HPLC analysis of MeOH extracts of the two samples showed that chlorogenic acid, rutin, and propyl gallate are major compounds in the coastal sample, while vanilin, quercetin, and 4′,7-dihydroxyisoflavone are majors in the inland one. The EOs showed considerable phytotoxicity against C. murale with IC50 value of 2.66, 0.59, and 0.70 mg mL−1 for germination, root, and shoot growth, respectively from the inland sample. While the coastal sample attained the IC50 values of 1.58, 0.45, and 0.66 mg mL−1. MeOH extracts revealed stronger antioxidant activity compared to the EOs. Based on IC50 values, the ascorbic acid revealed 3-fold of the antioxidant compared to the EO of the coastal sample and 4-fold regarding the inland sample. However, the ascorbic acid showed 3-fold of the antioxidant activity of the MeOH extracts of coastal and inland samples. Although H. curassavicum is considered as a noxious, invasive plant, the present study revealed that EO and MeOH extracts of the H. curassavicum could be considered as promising, eco-friendly, natural resources for antioxidants as well as weed control, particularly against the weed, C. murale.


2009 ◽  
Vol 35 (2) ◽  
pp. 49-52 ◽  
Author(s):  
Mohammad Mamun Hossain ◽  
Sukanta Kumar Shaha ◽  
Foysal Aziz

Nitrogen containing heterocyclic compounds such as oxindoles especially isatins and their derivatives have excellent biological properties such as anti-inflammatory, antimicrobial, anticancer activity. In addition, this is first time ever we would like to report the antioxidant properties of the said class of compounds. To determine such an important bioactivity a number of halogenated oxindoles 1-3, their diaza-biphenyl derivatives (4-6), ring extended biphenyl (7) and bis-amide (8) were synthesized by classical heating method. The antioxidant activity of all the synthesized compounds was screened by DPPH method with respect to ascorbic acid. In our present investigation some of the synthesized compounds (1, 2, 3, 5 and 6) were found to be active.Keywords: Antioxidant; Isatin; OxindoleOnline 12 August 2009DOI: 10.3329/bmrcb.v35i2.2564Bangladesh Med Res Counc Bull 2009; 35: 49-52 


2017 ◽  
Vol 13 (12) ◽  
pp. 323
Author(s):  
Rachid Ismaili ◽  
Sara Houbairi ◽  
Lanouari Sanâa ◽  
Moustaid Khadija ◽  
Lamiri Abdeslam

Morocco is a traditional supplier of medicinal and aromatic plants to the world. This activity has resulted to the exploitation of wild plants than dried plants for herbal needs and food flavors. Many species are used for the production of plant extracts and other aromatic essences mainly for the pharmaceutical and cosmetics industry. Among these metabolites, there are essential oils that are mixtures of aromatic substances present in the form of tiny droplets in the leaves, fruit peel, resin, branches, and wood. These oils, however, is endowed with antioxidant properties. This study aims to evaluate the antioxidant activity of essential oils of three moroccan aromatic and medicinal plants: Thymus vulgaris, Mentha spicata, and Citrus limonum. The oil extraction was performed by steam distillation in a Clevenger type apparatus. Also, the antioxidant activity of these oils was evaluated by the method of DPPH (2,2-diphenyl-1-picrylhydrazyl), in comparison with the antioxidant synthetic, ascorbic acid. In the present work, the results showed that the antioxidant activity of three essential oils studied by the trapping method of free radical is moderate. Also, the oil of Thymus vulgaris has a strong activity which exceeds that of ascorbic acid. This was followed by the oil of Mentha spicata, while the lowest activity was observed in Citrus limonum.


2021 ◽  
Vol 11 (1) ◽  
pp. 19
Author(s):  
Virdah Dwi Dewaantari ◽  
Setyabudi Setyabudi ◽  
Kun Ismiyatin

Background: Free radicals are molecules without any electron pairs, unstable, and highly reactive. Antioxidants are needed to reduce free radicals. Antioxidants provide various benefits in dentistry as a preventive agent for caries, healing, bone formation, mouthwash, preventive and therapeutic cancer, and reduction of periodontal disease progressions. Additionally, research on antioxidants is still undergone due to the existence of free radical residues on bleached teeth. Epigallocatechin-3-gallate (EGCG), ascorbic acid (AA), and sodium ascorbate (SA) are ingridients that have antioxidant properties. Antioxidants can be in two forms solution and gel. Solutions have a higher substance releasing power than gel. Gel is extremely adhesive, so it does not flow easily. Meanwhile, solutions are rather unstable because it flows easily. Antioxidant activities were evaluated with 2,2-diphenyl-1-picrylhydrazyl (DPPH) Assay method. Purpose: Analyzing through literature reviews the potential antioxidants of EGCG, AA, and SA in solution and gel forms by DPPH Assay. Reviews: There were eight journal articles used in this review. The first article described antioxidant solutions in which EGCG was higher than sodium ascorbate. The second article showed that antioxidant gel EGCG was lower than sodium ascorbate. The third journal explained that ascorbic acid was higher than sodium ascorbate both in solution and gel forms. The other articles provided some information about the antioxidant activity percentages of EGCG, SA, and AA in the forms of gel and solutions by DPPH assay. Conclusion: EGCG has a higher antioxidant activity than SA, but it is lower when compared to AA in both solution and gel forms by DPPH assay.


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