Effects of pH on Nanofibrillation of TEMPO-Oxidized Paper Mulberry Bast Fibers

TEMPO oxidation was conducted as a pretreatment to achieve efficient nanofibrillation of long paper mulberry bast fibers (PMBFs). The pH dependency of nanofibrillation efficiency and the characteristics of the resulting cellulose nanofibrils (CNFs) were investigated. As the pH increased, the negative value of the zeta potential of TEMPO-oxidized fibers increased. The increase in electrostatic repulsion at pH values of greater than 9 prevented the entanglement of long PMBFs, which was a drawback for defibrillation at acidic pH. With increasing pH, the CNF production yield was increased. The crystallinity index of TEMPO-oxidized CNFs from PMBFs was 83.5%, which was higher than that of TEMPO-oxidized CNFs from softwood fibers in the same conditions. The tensile strength of nanopaper from TEMPO-oxidized PMBF CNFs was 110.18 MPa, which was approximately 30% higher than that (84.19 MPa) of the TEMPO-oxidized CNFs from softwood fibers.

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Mechanisms of Heat-induced Antigen Retrieval: Does pH or Ionic Strength of the Solution Play a Role for Refolding Antigens?

Journal of Histochemistry & Cytochemistry ◽

10.1369/jhc.5c6627.2005 ◽

2005 ◽

Vol 53 (11) ◽

pp. 1311-1321 ◽

Cited By ~ 37

Author(s):

Katsura Emoto ◽

Shuji Yamashita ◽

Yasunori Okada

Keyword(s):

Ionic Strength ◽

The Other ◽

Antigen Retrieval ◽

Antigenic Determinants ◽

Acidic Ph ◽

Cooling Process ◽

Ph Values ◽

Ph Dependency ◽

Nacl Concentration ◽

Effects Of Ph

We investigated the effects of pH and ionic strength of solutions used for antigen retrieval to elucidate the mechanism of heat-induced antigen retrieval (HIAR) in immunohistochemistry. The immunostaining intensity of nuclear, cytoplasmic, cell membrane, and extracellular matrix antigens with 17 different antibodies was evaluated in formaldehyde-fixed and paraffin-embedded mouse and human tissues. Deparaffinized sections were autoclaved for 10 min in buffers with different pH values ranging from 3.0 to 10.5. To test the influence of ionic strength on immunoreactions, the sections were autoclaved for 10 min in 20 mM Tris-HCl buffers (TB) at pH 9.0 and 10.5 with or without 25, 50, and 100 mM NaCl. There were two immunostaining patterns for pH dependency of HIAR. First, the majority of antibodies recovered their antigenicity when heated in the buffers with both acidic pH (pH 3.0) and basic pH (pH 9.0 and 10.5). Second, some antibodies showed strong immunostaining only at basic pH values (pH 9.0 and 10.5). When the sections were autoclaved in TB at pH 9.0, immunostaining of all eight antibodies examined decreased as the NaCl concentration increased. On the other hand, when the sections were treated with TB at pH 10.5, all antibodies yielded stronger reactions in the buffer containing NaCl than in the buffer without NaCl; five antibodies exhibited the strongest immunoreaction at concentrations from 25 to 50 mM. These results suggest that the extended polypeptides by heating are charged negatively or positively at basic or acidic pH, and that an electrostatic repulsion force acts to prevent random entangling of polypeptides caused by hydrophobic attractive force and to expose antigenic determinants, during cooling process of HIAR solution.

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The Effect of Deinking Process on Bioethanol Production from Waste Banknote Paper

Processes ◽

10.3390/pr8121563 ◽

2020 ◽

Vol 8 (12) ◽

pp. 1563

Author(s):

Omid Yazdani Aghmashhadi ◽

Ghasem Asadpour ◽

Esmaeil Rasooly Garmaroody ◽

Majid Zabihzadeh ◽

Lisandra Rocha-Meneses ◽

...

Keyword(s):

Tensile Strength ◽

Enzymatic Hydrolysis ◽

Crystallinity Index ◽

Sugar Concentration ◽

Waste Paper ◽

Process Efficiency ◽

Bioethanol Production ◽

Ph Values ◽

Optimal Values ◽

Chemical Pretreatments

The aim of this paper is to study the effect of reinking and pretreatment of waste banknote paper on its usability in the bioethanol production process. To this end, the tensile strength of worn banknote paper was first studied at different pH values. The sample with the lowest tensile strength was considered for the next sections. In the deinking process, NaOH at different concentrations (1%, 2%, 3%, and 4%) and in combination with ultrasonic treatment was applied. After deinking the pulp, two acidic and alkaline chemical pretreatments with concentrations of 1%, 2%, 3%, and 4% were used independently and in combination with ultrasonic. Enzymatic hydrolysis, following fermentation with Scheffersomyces stipitis, and crystallinity measurements were used to confirm the efficiency of the pretreatments. RSM Design Expert software was used to determine the optimal values by considering the three variables—enzyme loading, ultrasonic loading, and contact time for waste paper deinked (WPD) and waste paper blank (WPB) pulps. The results indicated that repulping was the most efficient at pH = 2. In deinking, the highest brightness was obtained using 3% NaOH in combination with ultrasonic. Between the acid and alkaline pretreatment, the acid treatment was more appropriate according to the resulting sugar concentration and weight loss. XRD tests confirmed that the lowest crystallinity index was obtained in the sample pretreated with 4% sulfuric acid in combination with ultrasonic. The highest sugar concentration in the enzymatic hydrolysis step was 92 g/L for WPD and 81 g/L for WPB. For the fermentation at 96 h, the highest ethanol concentration and process efficiency achieved were 38 g/L and 80.9% for WPD and 31 g/L and 75.04% for WPB, respectively. Our research shows that the deinking process can widen the utilization potential of waste banknote paper in biorefinery processes.

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The Effects of pH and Temperature on Cysteine Protease (Cathepsin B) Activity in Miracidia and Eggs of Fasciola hepatica

Iranian Journal of Parasitology ◽

10.18502/ijpa.v15i2.3305 ◽

2020 ◽

Author(s):

Leila KIANIFARD ◽

Mohammad YAKHCHALI ◽

Mehdi IMANI

Keyword(s):

Cysteine Protease ◽

Cathepsin B ◽

Fasciola Hepatica ◽

Distilled Water ◽

Acidic Ph ◽

Optimum Temperature ◽

Egg Hatching ◽

Ph Values ◽

Zoonotic Parasite ◽

Effects Of Ph

Abstract Background: Fascioliasis is a worldwide zoonotic disease caused by the trematodes Fasciola hepatica in humans and animals. Proteases are essential for the survival of parasites and have important activities such as penetration, tissue migration, and egg hatching. This study was conducted to analyze cysteine protease of the miracidia and eggs of F. hepatica, and to assess the effects of pH and temperature on the proteases activity and stability. Methods: Adults F. hepatica were isolated from infected livers and were morphologically identified in 2018. Eggs collected from the adults and incubated in distilled water at 28 °C for 16 d to produce miracidia. The extract was collected from miracidia and eggs. A substrate for cathepsin B (Z-Arg-Arg-Pna) was used to assess the enzyme activity at different (2-12) pH levels. After homogenization, protein level was measured with Bradford method. Estimation of optimum temperature and pH was performed in the temperature range of 10-90 ° C and pH values from 2-12. Results: The highest activity of the miracidia and eggs enzyme extracts for Z-ArgArg-pNA was at pH 4. The miracidia extract was most stable at neutral pH and the eggs extract was most stable in acidic pH. The optimum temperature activity for both stages was 40 °C. These proteases were stable up to 40 °C. Conclusion: Upon the importance of pH and temperature in the life cycle of F. hepatica, the current findings can be used for induction of some modifications in pH and preventing the activity of the enzymes for decrement of the efficacy of miracidia penetration into the intermediate snails and egg hatching of this zoonotic parasite.

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Effects of pH Values on the Physicochemical Properties and Photocatalytic Activities of Nitrogen-doped TiO2

Journal of Inorganic Materials ◽

10.3724/sp.j.1077.2008.00387 ◽

2008 ◽

Vol 23 (2) ◽

pp. 387-392 ◽

Cited By ~ 2

Author(s):

Yuan BAI

Keyword(s):

Physicochemical Properties ◽

Nitrogen Doped ◽

Ph Values ◽

Photocatalytic Activities ◽

Effects Of Ph

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Is the Acidic pH of the External Auditory Canal Playing a Significant Role in the Treatment of Acute Otitis Externa?

Revista de Chimie ◽

10.37358/rc.18.8.6521 ◽

2018 ◽

Vol 69 (8) ◽

pp. 2304-2305

Author(s):

Oana Ruxandra Iana ◽

Dragos Cristian Stefanescu ◽

Viorel Zainea ◽

Razvan Hainarosie

Keyword(s):

External Auditory Canal ◽

Otitis Externa ◽

Proton Pumps ◽

Acidic Ph ◽

External Ear ◽

Ph Values ◽

The World ◽

Subcutaneous Tissues ◽

Low Levels ◽

Value Changes

Variable pH values for skin have been reported in the literature, all within the acidic range, varying from 4.0 up to 7. 0. The origin of the acidic pH remains conjectural, and several factors have been incriminated with this role, such as eccrine and sebaceous secretions as well as proton pumps. Keeping low levels of pH prevents microbial dispersal as well as multiplication. The skin in the external auditory canal is also covered with this acidic mantle with antimicrobial value. Changes of pH in the external ear can lead to acute otitis externa. This condition is defined by the inflammation and infection of the cutaneous and subcutaneous tissues of the external auditory canal. 10% of the world�s population may suffer from acute otitis externa at least once in their lifetime. This paper aims to consolidate the relevance of an acidic pH in the healthy external ear and its relation to the pathogenesis and treatment of otitis externa through a prospective and interventional clinical study on 80 patients who presented to the outpatient department at Prof. Dr D. Hociota ENT Institute in Buch

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Nitrogen Removal from Anaerobically-Treated Leachate

Water Quality Research Journal ◽

10.2166/wqrj.1984.008 ◽

1984 ◽

Vol 19 (1) ◽

pp. 87-100

Author(s):

D. Prasad ◽

J.G. Henry ◽

P. Elefsiniotis

Keyword(s):

Nitrogen Removal ◽

Air Flow ◽

Operating Conditions ◽

Air Flow Rate ◽

Flow Rates ◽

Anaerobic Filter ◽

Ph Values ◽

Wide Range ◽

Ammonia Desorption ◽

Effects Of Ph

Abstract Laboratory studies were conducted to demonstrate the effectiveness of diffused aeration for the removal of ammonia from the effluent of an anaerobic filter treating leachate. The effects of pH, temperature and air flow on the process were studied. The coefficient of desorption of ammonia, KD for the anaerobic filter effluent (TKN 75 mg/L with NH3-N 88%) was determined at pH values of 9, 10 and 11, temperatures of 10, 15, 20, 30 and 35°C, and air flow rates of 50, 120, and 190 cm3/sec/L. Results indicated that nitrogen removal from the effluent of anaerobic filters by ammonia desorption was feasible. Removals exceeding 90% were obtained with 8 hours aeration at pH of 10, a temperature of 20°C, and an air flow rate of 190 cm3/sec/L. Ammonia desorption coefficients, KD, determined at other temperatures and air flow rates can be used to predict ammonia removals under a wide range of operating conditions.

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Valorization of Byproducts of Hemp Multipurpose Crop: Short Non-Aligned Bast Fibers as a Source of Nanocellulose

Molecules ◽

10.3390/molecules26164723 ◽

2021 ◽

Vol 26 (16) ◽

pp. 4723

Author(s):

Sara Dalle Vacche ◽

Vijayaletchumy Karunakaran ◽

Alessia Patrucco ◽

Marina Zoccola ◽

Loreleï Douard ◽

...

Keyword(s):

Infrared Spectroscopy ◽

Thermogravimetric Analysis ◽

Cannabis Sativa ◽

Crystallinity Index ◽

Residual Lignin ◽

Chemical Pretreatment ◽

X Ray Diffraction ◽

X Ray ◽

Bast Fibers ◽

Seed Maturity

Nanocellulose was extracted from short bast fibers, from hemp (Cannabis sativa L.) plants harvested at seed maturity, non-retted, and mechanically decorticated in a defibering apparatus, giving non-aligned fibers. A chemical pretreatment with NaOH and HCl allowed the removal of most of the non-cellulosic components of the fibers. No bleaching was performed. The chemically pretreated fibers were then refined in a beater and treated with a cellulase enzyme, followed by mechanical defibrillation in an ultrafine friction grinder. The fibers were characterized by microscopy, infrared spectroscopy, thermogravimetric analysis and X-ray diffraction after each step of the process to understand the evolution of their morphology and composition. The obtained nanocellulose suspension was composed of short nanofibrils with widths of 5–12 nm, stacks of nanofibrils with widths of 20–200 nm, and some larger fibers. The crystallinity index was found to increase from 74% for the raw fibers to 80% for the nanocellulose. The nanocellulose retained a yellowish color, indicating the presence of some residual lignin. The properties of the nanopaper prepared with the hemp nanocellulose were similar to those of nanopapers prepared with wood pulp-derived rod-like nanofibrils.

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In Vitro Incorporation of Helicobacter pylori into Candida albicans Caused by Acidic pH Stress

Pathogens ◽

10.3390/pathogens9060489 ◽

2020 ◽

Vol 9 (6) ◽

pp. 489 ◽

Cited By ~ 1

Author(s):

Kimberly Sánchez-Alonzo ◽

Cristian Parra-Sepúlveda ◽

Samuel Vega ◽

Humberto Bernasconi ◽

Víctor L. Campos ◽

...

Keyword(s):

Helicobacter Pylori ◽

Candida Albicans ◽

Pcr Amplification ◽

Growth Curves ◽

Acidic Ph ◽

Ph Values ◽

16S Gene ◽

Wide Range ◽

H Pylori

Yeasts can adapt to a wide range of pH fluctuations (2 to 10), while Helicobacter pylori, a facultative intracellular bacterium, can adapt to a range from pH 6 to 8. This work analyzed if H. pylori J99 can protect itself from acidic pH by entering into Candida albicans ATCC 90028. Growth curves were determined for H. pylori and C. albicans at pH 3, 4, and 7. Both microorganisms were co-incubated at the same pH values, and the presence of intra-yeast bacteria was evaluated. Intra-yeast bacteria-like bodies were detected using wet mounting, and intra-yeast binding of anti-H. pylori antibodies was detected using immunofluorescence. The presence of the H. pylori rDNA 16S gene in total DNA from yeasts was demonstrated after PCR amplification. H. pylori showed larger death percentages at pH 3 and 4 than at pH 7. On the contrary, the viability of the yeast was not affected by any of the pHs evaluated. H. pylori entered into C. albicans at all the pH values assayed but to a greater extent at unfavorable pH values (pH 3 or 4, p = 0.014 and p = 0.001, respectively). In conclusion, it is possible to suggest that H. pylori can shelter itself within C. albicans under unfavorable pH conditions.

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Effects of pH and ionic strength on the cation exchange capacity of soils with variable charge

Soil Research ◽

10.1071/sr9810093 ◽

1981 ◽

Vol 19 (1) ◽

pp. 93 ◽

Cited By ~ 30

Author(s):

GP Gillman

Keyword(s):

Ionic Strength ◽

Cation Exchange ◽

Cation Exchange Capacity ◽

Exchange Capacity ◽

Surface Soils ◽

Ph Values ◽

Variable Charge ◽

Effects Of Ph ◽

Variable Charge Soils

The cation exchange capacity of six surface soils from north Queensland and Hawaii has been measured over a range of pH values (4-6) and ionic strength values (0.003-0.05). The results show that for variable charge soils, modest changes in electrolyte ionic strength are as important in their effect on caton exchange capacity as are changes in pH values.

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