scholarly journals Earthworm (Eisenia fetida) Mucus Inspired Bionic Fertilizer to Stimulate Maize (Zea mays L.) Growth

2021 ◽  
Vol 13 (8) ◽  
pp. 4299
Author(s):  
Zhihong Zhang ◽  
Yali Wu ◽  
Vi Khanh Truong ◽  
Dongguang Zhang
Keyword(s):  
Amino Acids ◽  
Eisenia Fetida ◽  
Seed Vigor ◽  
Control Group ◽  
Fertilizer Treatment ◽  
Dry Matter Accumulation ◽  
Liquid Fertilizer ◽  
Maize Growth ◽  
Emergence Rate ◽  
Epidermal Mucus

Background: Increasing population and food consumption are placing unprecedented demands on crop production. Maize is one of the most important food crops in the world, the improvement of its yield primarily depends on the application of chemical fertilizer. Methods: Earthworm activity is an essential factor in promoting soil fertility and stimulating plant growth. Inspired by amino acids composition of earthworm (Eisenia fetida) epidermal mucus, the liquid fertilizer was developed and prepared by utilizing a bionic approach. The influence of earthworm epidermal mucus (mucus), the mucus-mimicked mixture of amino acids (bionic fertilizer) and urea fertilizer (urea) on maize emergence and growth were studied and compared with the control group (distilled water). Experimental cultivation tests were conducted. The aforementioned three types of liquid fertilizer effects on maize seed vigor index, seedling emergence rate and plant quality were quantitatively evaluated. Results: Based on the conducted research, it was found that the beneficial effects of different fertilizers for maize emergence rate were ranked as follows: mucus > bionic fertilizer > urea. The low concentration treatments were beneficial to the maize emergence, while the high concentration treatments were helpful to the maize growth and root development. Besides, the lower concentration of mucus was the most effective fertilizer treatment for improving seedling quality. In addition, the test results of three types of liquid fertilizer effects on maize growth indicated that the higher concentration treatments provided more nitrogen nutrition than lower concentration treatments. Furthermore, the maize stem height and diameter were significantly promoted (p < 0.05) by the three types of liquid fertilizer. The beneficial influences of liquid fertilizer treatments for plant height, stem diameter, relative chlorophyll content and photosynthetic characteristic of leaves were ranked as follows: bionic fertilizer > urea > mucus. Conclusions: Bionic fertilizer demonstrated significant beneficial fertilizing effects (p < 0.05), which increased soil nutrients, improved maize physiological parameters, promote its growth and improved dry matter accumulation. The tested results verified the effectiveness of bionic fertilizer on stimulating maize growth.

Plasma-Free Amino Acid Profiling of Nasal Polyposis Patients

2020 ◽  
Vol 22 (9) ◽  
pp. 657-662 ◽  
Author(s):  
Mustafa Celik ◽  
Alper Şen ◽  
İsmail Koyuncu ◽  
Ataman Gönel
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Free Amino Acid ◽  
Free Amino ◽  
Nasal Polyposis ◽  
Amino Acid Profile ◽  
Healthy Control Group ◽  
Control Group ◽  
Healthy Control ◽  
History Of

Aim and Objective:: To determine the mechanisms present in the etiopathogenesis of nasal polyposis. It is not clear whether amino acids contribute in a causal way to the development of the disease. Therefore, the aim of this study was to determine the plasma-free amino acid profile in patients with nasal polyposis and to compare the results with a healthy control group. Materials and Methods:: This was a prospective controlled study that took place in the Otolaryngology Department at the Harran University Faculty of Medicine between April 2017 and April 2018. Plasmafree amino acid profile levels were studied in serum samples taken from a patient group and a healthy control group. Patients who were diagnosed with bilateral diffuse nasal polyposis and were scheduled for surgical interventions were included in this study. Individuals whose age, gender, and body mass index values were compatible with that of the patient group and who did not have any health problems were included in the control group. All the participants whose levels of plasma-free amino acid were thought to be affected by one or more of the following factors were excluded from the study: smoking and alcohol use, allergic rhinitis presence, the presence of acute or chronic sinusitis, a history of endoscopic sinus surgery, unilateral nasal masses, a history of chronic drug use, systemic or topical steroid use in the last three months for any reason, and liver, kidney, hematological, cardiovascular, metabolic, neurological, or psychiatric disorders or malignancies. Results: In patients with nasal polyposis, 3-methyl histidine (3-MHIS: nasal polyposis group (ng) = 3.22 (1.92 – 6.07); control group (cg) = 1.21 (0.77 – 1.68); p = 0.001); arginine (arg: ng = 98.95 (70.81 – 117.75); cg = 75.10 (54.49 – 79.88); p = 0.005); asparagine (asn: ng = 79.84 (57.50 – 101.44); cg = 60.66 (46.39 – 74.62); p = 0.021); citrulline (cit: ng = 51.83 (43.81 – 59.78); cg = 38.33 (27.81 – 53.73); p = 0.038); cystine (cys: ng = 4.29 (2.43 – 6.66); cg = 2.41 (1.51 – 4.16); p = 0.019); glutamic acid (glu: ng = 234.86 (128.75 – 286.66); cg = 152.37 (122.51 – 188.34); p = 0.045); histidine (his: ng = 94.19 (79.34 – 113.99); cg = 74.80 (62.76 – 98.91); p = 0.018); lysine (lys: ng = 297.22 (206.55 – 371.25); cg = 179.50 (151.58 – 238.02); p = 0.001); ornithine (ng = 160.62 (128.36 – 189.32); cg = 115.91 (97.03 – 159.91); p = 0.019); serine (ser: ng = 195.15 (151.58 – 253.07); cg = 83.07 (67.44 – 92.44); p = 0.001); taurine (tau: ng = 74.69 (47.00 – 112.13); cg = 53.14 (33.57 – 67.31); p = 0.006); tryptophan (trp: ng = 52.31 (33.81 – 80.11); cg = 34.44 (25.94 – 43.07); p = 0.005), homocitrulline (ng = 1.75 (1.27 – 2.59); cg = 0.00 (0.00 – 0.53); p = 0.001); norvaline (ng = 6.90 (5.61 – 9.18); cg = 4.93 (3.74 – 7.13); p = 0.021); argininosuccinic acid (ng = 14.33 (10.06 – 25.65); cg = 12.22 (5.77 – 16.87) p = 0.046); and plasma concentrations were significantly higher than in the healthy control group (p <0.05). However, the gamma-aminobutyric acid (gaba: ng = 0.16 (0.10 – 0.24); cg = 0.21 (0.19 – 0.29); p = 0.010) plasma concentration was significantly lower in the nasal polyposis group than in the healthy control group. Conclusion: In this study, plasma levels of 15 free amino acids were significantly higher in the nasal polyposis group than in the healthy control group. A plasma level of 1 free amino acid was found to be significantly lower in the nasal polyposis group compared to the healthy control group. Therefore, it is important to determine the possibility of using the information obtained to prevent the recurrence of the condition and to develop effective treatment strategies. This study may be a milestone for studies of this subject. However, this study needs to be confirmed by further studies conducted in a larger series.

scholarly journals Plasma Branched-Chain Amino Acids and Incident Cardiovascular Disease in the PREDIMED Trial

2016 ◽  
Vol 62 (4) ◽  
pp. 582-592 ◽  
Author(s):  
Miguel Ruiz-Canela ◽  
Estefania Toledo ◽  
Clary B Clish ◽  
Adela Hruby ◽  
Liming Liang ◽  
...  
Keyword(s):  
Cardiovascular Disease ◽  
Amino Acids ◽  
Cardiovascular Death ◽  
Branched Chain Amino Acids ◽  
Control Group ◽  
Cvd Risk ◽  
Hazard Ratios ◽  
Increased Risk ◽  
Branched Chain

Abstract BACKGROUND The role of branched-chain amino acids (BCAAs) in cardiovascular disease (CVD) remains poorly understood. We hypothesized that baseline BCAA concentrations predict future risk of CVD and that a Mediterranean diet (MedDiet) intervention may counteract this effect. METHODS We developed a case-cohort study within the Prevención con Dieta Mediterránea (PREDIMED), with 226 incident CVD cases and 744 noncases. We used LC-MS/MS to measure plasma BCAAs (leucine, isoleucine, and valine), both at baseline and after 1 year of follow-up. The primary outcome was a composite of incident stroke, myocardial infarction, or cardiovascular death. RESULTS After adjustment for potential confounders, baseline leucine and isoleucine concentrations were associated with higher CVD risk: the hazard ratios (HRs) for the highest vs lowest quartile were 1.70 (95% CI, 1.05–2.76) and 2.09 (1.27–3.44), respectively. Stronger associations were found for stroke. For both CVD and stroke, we found higher HRs across successive quartiles of BCAAs in the control group than in the MedDiet groups. With stroke as the outcome, a significant interaction (P = 0.009) between baseline BCAA score and intervention with MedDiet was observed. No significant effect of the intervention on 1-year changes in BCAAs or any association between 1-year changes in BCAAs and CVD were observed. CONCLUSIONS Higher concentrations of baseline BCAAs were associated with increased risk of CVD, especially stroke, in a high cardiovascular risk population. A Mediterranean-style diet had a negligible effect on 1-year changes in BCAAs, but it may counteract the harmful effects of BCAAs on stroke.

scholarly journals Substitution of Chemical Fertilizer Using Natural Hormone and Cow Biourine On Growth and Yield of Shallot (Allium Cepa L.)

2020 ◽  
Vol 3 ◽  
pp. 43-47
Author(s):  
Lagiman ◽  
Ihvan Mei Nugraha ◽  
Ami Suryawati ◽  
Supono Budi Sutoto
Keyword(s):  
Block Design ◽  
Chemical Fertilizer ◽  
Coconut Water ◽  
Growth And Yield ◽  
Control Group ◽  
Fertilizer Treatment ◽  
Chemical Fertilizers ◽  
Natural Hormone ◽  
Stem Extract ◽  
Banana Stem

The use of natural hormone of sprout extract, coconut water, and banana stem extract and application of cow biourine has been introduced as an innovation to reduce the use of chemical fertilizers to increase the productivity of shallot. This study aims to compare the growth and yield of shallot with chemical fertilizer treatment and that of shallot treated with combination of natural hormone and cow biourine. The study was conducted in Temon Wetan, Temon, Kulon Progo, Special Region of Yogyakarta using a Complete Randomized Block Design, which consists of two factors and one control (chemical fertilizer). The first factor is a natural hormone: sprout extract, banana stem extract and coconut water. The second factor is the concentration of cow biourine: 30%, 40%, and 50%. Data were analyzed using diversity at 5% level, Orthogonal Contras at 5% level and DMRT Test with 5% level. It was revealed that the control group with a combined treatments was not significantly different from the growth and yield of shallot of the group with chemical fertilizers. Types of treatments with natural hormone and cow biourine concentrations provided the same growth and yield as that of chemical fertilizer.

scholarly journals Effect of Bokashi Fertilizer and Green Tonik Liquid Fertilizer on the Growth and Yield of Long Bean (Vigna sinensisL.)Parade Tavi Variety

AGRIFOR ◽  
2018 ◽  
Vol 17 (1) ◽  
pp. 81
Author(s):  
Elisabeth Elisabeth ◽  
Puji Astuti
Keyword(s):  
Fertilizer Application ◽  
Factorial Experiment ◽  
Bean Plant ◽  
Growth And Yield ◽  
Fertilizer Treatment ◽  
Fertilizer N ◽  
Liquid Fertilizer ◽  
Randomized Design ◽  
Interaction Treatment ◽  
Completely Randomized Design

The purpose of the research is to study the effect of bokashi fertilizer and Green Tonik liquid fertilizer and its interaction on the growth and yield of long bean plant, as well as to find the proper dosage of bokashi fertilizer and Green Tonik liquid fertilizer concentration for obtaining the best yield of long bean.The research was conducted using Completely Randomized Design (CRD) in 4 x 4 Factorial Experiment and four replications.  The factor was the dosage of bokashi fertilizer (B) consisting of 4 levels : no bokashi fertilizer application (b0), 100 g/polybag(b1), 200 g/polybag (b2), and 300 g/polybag(b3).   The second factor was the concentration of Green Tonik liquid fertilizer (N) consisting 4 levels : no Green Tonikfertilizer application (n0), 2 ml/ l water (n1),  4 ml/l water (n2), and 6 ml/lwater (n3).The results showed that: (1) the bokashi fertilizer treatment affected significantly on the plant lenght at age 15 days after planting, number of pods and weight of pods per plant, but it did not affect significantly on the plant lenght at age 22, 29 and 36 days after planting, age of plants flowered, and pods lenght; (2)the Green Tonik liquid treatment affected significantly on the plant lenght at age 22, 29, and 60 days after plantingand weight of pods per plant, but it did not affect significantly on the plant lenght at age 15 days after planting, age of plant flowered, and pods lenght;  (3) the interaction treatment between bokashi fertilizer and Green Tonik liquid fertilizer affected significantly on the plant lenght at age 15 days after planting, pods lenght, and weight of pods per plant, but did not affect significantly on the plant lenght at age 22, 29, and 36 after planting, age of plant flowered, and number of pods; and (4) the weighest weight of pods per plant was produced in b2n3 treatment of 401,75 g/plant, while the lighest one was produced in b0n0 treatment of 145,75 g/plant.

scholarly journals АМИНОКИСЛОТНЫЙ СОСТАВ МЯСА ЦЫПЛЯТ-БРОЙЛЕРОВ ПРИ ПРИМЕНЕНИИКОРМОВЫХ ДОБАВОК «АБИОТОНИК» И «ЧИКТОНИК»

2019 ◽  
pp. 10-15
Author(s):  
F.I. Vasilevich ◽  
V.M. Bachinskaya ◽  
Yu.V. Petrova
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Broiler Chickens ◽  
Live Weight ◽  
Feed Additives ◽  
Poultry Meat ◽  
Control Group ◽  
Normal Range

Экспериментальные исследования кормовых добавок Абиотоник и Чиктоник проводили на базе вивария кафедры эпизоотологии и организации ветеринарного дела, а ветеринарносанитарную экспертизу продуктов убоя цыплятбройлеров проводили на кафедре паразитологии и ветеринарносанитарной экспертизы ФГБОУ ВО МГАВМиБ МВА имени К.И. Скрябина и ФГБНУ ФНЦ ВИЭВ РАН, аминокислотный состав мяса перепелов в Государственном бюджетном учреждении Краснодарского края Кропоткинская краевая ветеринарная лаборатория . Из цыплят в суточном возрасте кросса Кобб500 было сформировано три группы по 10 голов в каждой две опытные и контрольная опытным группам выпаивали кормовые добавки из расчета 1 мл/кг живой массы птицы до 50 суток выращивания, убой птицы проводили на 56 сутки. Тушки птицы после 24х часов созревания в холодильной камере при температуре 4 С подвергали исследованиям по общепринятым методикам: ГОСТ Р 519442002. Мясо птицы. Методы органолептических показателей, температуры и массы ГОСТ 314702012. Мясо птицы, субпродукты и полуфабрикаты из мяса птицы. Методы органолептических и физикохимических исследований аминокислотный состав мяса исследовали согласно М 0438 2009. Корма, комбикорма и сырье для их производства. Методика измерений массовой доли аминокислот методом капиллярного электрофореза с использованием системы капиллярного электрофореза Капель. Применение кормовых добавок в дозе 1 мл/кг живой массы способствовало увеличению живой массы птицы при применении Абиотоника на 54,23, а при применении Чиктоника на 37,70 по отношению к контролю. Во всех исследуемых пробах количество ЛЖК находится в пределах нормы и составило: в 1й опытной 1,390,03 мг КОН, во 2й опытной 1,420,04 мг КОН и в контрольной группе 1,810,06 мг КОН, что говорит о свежести и доброкачественности мяса. Значение рН мяса цыплятбройлеров находилось в трех группах в пределах нормы и не превышало 6,0. По результатам проведенных исследований аминокислотного состава красной и белой мышечной ткани цыплятбройлеров было установлено, что применение кормовой добавки Абиотоник способствовало увеличению незаменимых аминокислот на 12,14 и на 22,84 соответственно, а заменимых на 8,11 и на 22,51 по отношению к контрольной группеExperimental studies of feed additives Abiotonik and Chiktonik were conducted on the basis of the vivarium of the Department of Epizootology and Organization of Veterinary, and the veterinarysanitary examination of the products of slaughter broiler chickens was carried out at the Department of Parasitology and VeterinarySanitary Expertise of FSBEI HE MGAVMiB MBA named after KI Scriabin and the FSBI of the Federal Research Center of the VIEW RAS, the amino acid composition of quail meat in the State budget institution of the Krasnodar Territory Kropotkinskaya regional veterinary laboratory. Three groups of 10 animals each were formed from chickens at the daily age of the Cobb500 crosscountry, the experimental groups were fed feed additives at the rate of 1 ml / kg of live weight of poultry for up to 50 days of cultivation, and poultry were slaughtered for 56 days. Poultry carcasses after 24 hours of maturation in a refrigerating chamber at a temperature of 4 C were subjected to research according to generally accepted methods: GOST R 519442002. Poultry meat Methods of organoleptic characteristics, temperature and mass GOST 314702012 Poultry meat, offal and semifinished products from poultry meat. Methods of organoleptic and physicochemical studies) Amino acid composition of meat was carried out according to M 04382009. Feed, feed and raw materials for their production. Methods of measuring the mass fraction of amino acids by capillary electrophoresis using the Cappel capillary electrophoresis system. The use of feed additives in a dose of 1 ml / kg of live weight contributed to an increase in live weight of the bird when using Abiotonics by 54.23, and when using Chictonics by 37.70 relative to the control. In all studied samples, the number of VFAs is within the normal range and amounted to 1.39 0.03 mg KOH in 1 experimental group, 1.42 0.04 mg KOH in 2 experimental groups and 1.81 0.06 in the control group. mg KOH, which speaks of the freshness and goodness of meat. The pH of broiler chicken meat was in three groups within the normal range and did not exceed 6.0. According to the results of studies of the amino acid composition of red and white muscle tissue of broiler chickens, it was found that the use of the feed additive Abiotonik contributed to an increase in essential amino acids by 12.14 and by 22.84, and by replaceable ones by 8.11 and by 22.51 relative to the control group.Экспериментальные исследования кормовых добавок Абиотоник и Чиктоник проводили на базе вивария кафедры эпизоотологии и организации ветеринарного дела, а ветеринарносанитарную экспертизу продуктов убоя цыплятбройлеров проводили на кафедре паразитологии и ветеринарносанитарной экспертизы ФГБОУ ВО МГАВМиБ МВА имени К.И. Скрябина и ФГБНУ ФНЦ ВИЭВ РАН, аминокислотный состав мяса перепелов в Государственном бюджетном учреждении Краснодарского края Кропоткинская краевая ветеринарная лаборатория . Из цыплят в суточном возрасте кросса Кобб500 было сформировано три группы по 10 голов в каждой две опытные и контрольная опытным группам выпаивали кормовые добавки из расчета 1 мл/кг живой массы птицы до 50 суток выращивания, убой птицы проводили на 56 сутки. Тушки птицы после 24х часов созревания в холодильной камере при температуре 4 С подвергали исследованиям по общепринятым методикам: ГОСТ Р 519442002. Мясо птицы. Методы органолептических показателей, температуры и массы ГОСТ 314702012. Мясо птицы, субпродукты и полуфабрикаты из мяса птицы. Методы органолептических и физикохимических исследований аминокислотный состав мяса исследовали согласно М 0438 2009. Корма, комбикорма и сырье для их производства. Методика измерений массовой доли аминокислот методом капиллярного электрофореза с использованием системы капиллярного электрофореза Капель. Применение кормовых добавок в дозе 1 мл/кг живой массы способствовало увеличению живой массы птицы при применении Абиотоника на 54,23, а при применении Чиктоника на 37,70 по отношению к контролю. Во всех исследуемых пробах количество ЛЖК находится в пределах нормы и составило: в 1й опытной 1,390,03 мг КОН, во 2й опытной 1,420,04 мг КОН и в контрольной группе 1,810,06 мг КОН, что говорит о свежести и доброкачественности мяса. Значение рН мяса цыплятбройлеров находилось в трех группах в пределах нормы и не превышало 6,0. По результатам проведенных исследований аминокислотного состава красной и белой мышечной ткани цыплятбройлеров было установлено, что применение кормовой добавки Абиотоник способствовало увеличению незаменимых

scholarly journals Rearing larvae and juveniles of rainbow trout with different amino acid nutrition

2020 ◽  
Author(s):  
V. Kondratiuk ◽  
Keyword(s):  
Amino Acids ◽  
Rainbow Trout ◽  
Amino Acid ◽  
Juvenile Fish ◽  
Control Group ◽  
Economic Optimization ◽  
Larvae And Juveniles ◽  
Experimental Fish ◽  
Juvenile Trout ◽  
High Level

The article considers the efficiency of using complete compound feeds with different levels of lysine and methionine in rearing larvae and juveniles of rainbow trout. The purpose of the experiment was to establish the effect of different amino acid levels in nutrition of juvenile trout on its productivity. For this purpose, five experimental groups were formed by the method of analogues. The experiment lasted 35 days and was divided into two periods: equalizing (5 days) and main (30 days). During the equalizing period, the experimental fish consumed feed of the control group. In the main period, the levels of lysine and methionine in experimental feeds for different experimental groups of trout ranged from 2.9 to 3.3% and from 0.95 to 3.15%, respectively. It is proved that increasing the content of lysine and methionine in feed for larvae and juveniles of rainbow trout to the levels of 3.2 and 1.1%, respectively, promotes increase in their weight by 10.7% (p <0.01) and growth intensity - by 6.4-11.1%, while further increase in these amino acids content in the feed reduces the productivity of fish. Polynomial equations for determining the juvenile fish weight at any stage of rearing at a high level of determination for each of the experimental groups have been calculated. It was found that with an increase in the content of lysine and methionine in feed to the levels of 3.2 and 1.1%, respectively, reduces feed costs per 1 kg of weight gain in trout larvae and juveniles by 4.1%, and a further increase in levels of these amino acids to 3.3 and 1.15%, respectively, increase this index by 1.1%. The experimental fish survival ranged from 83.9 to 85.1%. In the production of trout according to the criteria of maximum productivity and to the economic optimization criteria, it is recommended to use complete feed with the level of lysine - 3.2% and methionine - 1.1% for feeding trout larvae and juveniles.

scholarly journals Dietary supplements based on modified and enriched with amino acids zeolite in the cultivation of young turkeys

2022 ◽  
Vol 354 (11-12) ◽  
pp. 20-23
Author(s):  
S. V. Dezhatkina ◽  
N. A. Feoktistova ◽  
E. V. Pankratova ◽  
N. A. Provorova ◽  
E. S. Salmina
Keyword(s):  
Amino Acids ◽  
Hematological Parameters ◽  
Live Weight ◽  
High Growth ◽  
Mineral Elements ◽  
High Growth Rate ◽  
The Body ◽  
Control Group ◽  
Modified Zeolite ◽  
Negative Effect

Relevance. Modern turkey crosses have a genetically determined high growth rate. These farm birds are very sensitive to even minor fluctuations in their diet, are susceptible to a deficiency of amino acids, protein, mineral elements and vitamins, which can contribute to a decrease in their productivity, the development of pathological processes and diseases. The use of modern technologies and scientific achievements has made it possible to prepare a new product — modified zeolite. This natural mineral is already free of impurities and microbes, with open pore windows, has a high ability to selectively exchange, and is obtained by various methods of activating zeolite in industrial conditions. At the same time, Ulyanovsk specialists have mastered the technology of enriching modified zeolite with useful substances.Methods. The aim of the work was to study the effect on the body of turkeys and the level of their productivity of additives based on modified zeolite enriched with amino acids. The experiment was organized for 60 days on 250 turkeys of the breed Hybrid Cread-mayker aged 55–60 days in the conditions of the Ulyanovsk region. The control group received the main ration (MR), the experimental group was given once a day in addition to the MR in the morning feeding in a mixture with mixed feed 5% additives of modified zeolite enriched with amino acids.Results. The inclusion of a supplement based on modified zeolite enriched with amino acids in the diet of turkeys does not have a negative effect on the hematological parameters of their blood, all indicators corresponded to the physiological norm. During the experiment in the 2nd group, the live weight of poultry was obtained larger by 8.70%, which amounted to 12,296 kg, respectively, more profit was obtained from the sale of turkey meat, total revenue was 133,210, and additional profit was 27,960 rubles, 2.41 rubles of profit was received for 1 ruble of costs. Тhe data indicate the normalization of mineral homeostasis in the body of turkeys and better absorption of nutrients in the feed diet.

scholarly journals Mitigating Potentials of Antioxidant-Rich Plants in Earthworms Exposed to Glyphosate

2021 ◽  
Author(s):  
Isaac O Ayanda ◽  
Tolulope O. Ajayi
Keyword(s):  
Dna Damage ◽  
Eisenia Fetida ◽  
Control Group ◽  
Soil Organisms ◽  
Antioxidant Responses ◽  
Ocimum Gratissimum ◽  
Telfairia Occidentalis ◽  
Enzyme Superoxide Dismutase ◽  
High Concentrations ◽  
Significant Mortality

Abstract Glyphosate is a non-selective herbicide that has adverse effects on non-target organisms. This present study investigated the toxic effects of glyphosate on earthworms and the potential of antioxidant-rich plants, Ocimum gratissimum and Telfairia occidentalis remediate these effects. Earthworms (Eisenia fetida) were placed into four groups and treated with concentrations of 1, 2 and 3% glyphosate in soil. The last group was a control group. The worms were collected on the 3rd, 7th and 14th, days post-exposure. During these intervals, the weight of the worms and activities of antioxidant enzyme - superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), malondialdehyde (MDA) were measured to determine the level of antioxidant responses. Furthermore, the percentage of DNA fragmentation was measured to assess the level of DNA damage. Compared with the control group, earthworms exposed to glyphosate and fed with Ocimum gratissimum and Telfairia occidentalis showed varying responses, with increased activities of CAT, SOD, GSH and reduced levels of MDA. Also, decreased fragmented DNA was observed in earthworm groups fed with Ocimum gratissimum and Telfairia occidentalis in comparison with the group treated exclusively with the herbicide. These results suggest that toxicity from glyphosate exposure significantly reduced oxidative damage, lipid peroxidation and DNA damage in Eisenia fetida by the antioxidant-rich plants. It is conceivable that soil organisms could suffer a significant mortality when exposed to high concentrations of glyphosate. The cultivation of these plants should be encouraged while caution should be exercised in the use of the herbicides.

scholarly journals The fluctuation of free amino acids in serum during acute ischemic stroke

2016 ◽  
Vol 29 (4) ◽  
pp. 151-154 ◽  
Author(s):  
Maria Szpetnar ◽  
Anna Hordyjewska ◽  
Iwona Malinowska ◽  
Piotr Golab ◽  
Jacek Kurzepa
Keyword(s):  
Amino Acids ◽  
Ischemic Stroke ◽  
Serum Level ◽  
Free Amino Acids ◽  
Free Amino ◽  
Simultaneous Increase ◽  
Control Group ◽  
Blood Collection ◽  
Control Serum ◽  
Time Point

Abstract Currently, little data exists regarding the involvement of free amino acids (AA) in the pathogenesis of ischemic stroke (IS). Thus, our objective was to study the degree of the degree of fluctuation of free amino acids level in serum during the acute phase of IS. The study consisted of eighteen patients (female/male: 10/8; age: 73.1 ± 4.1) with acute IS that was confirmed by way of computed tomography, while twelve sex and age matched individuals were assigned as control group. During the study period, the patients did not receive any supplemental amino acids therapy that could affect the obtained results. The venous blood was obtained after >3 hours fasting at two time-points; time-point 1 – at admission to the hospital; time-point 2 – on day 5 from stroke onset. The blood for control purposes was collected only once, and the blood collection at time-point 1 was done before thrombolytic treatment (nine patients). The amino acids were identified using the Amino Acids Analyser (AAA 400) by INGOS Corp., Praha, Czech Republic. Our results revealed a statistically significant increase of glutamate, cystine and methionine on day 1 of stroke, in comparison to control, whereas, proline level was decreased on day 1 of stroke – in comparison to control serum. On comparing day 5 to the initial day of IS, elevation was observed of levels of asparagine, glycine, tyrosine, arginine, threonine, valine, leucine and phenylalanine. It can be said, then, that ischemic stroke induces both essential and nonessential amino acid fluctuations. Moreover, the decrease in proline and glutamine serum level with the simultaneous increase in the concentration of branch chain amino acids, Glu and Thr suggests a violent mobilization of the body’s proteins. Thus, a decrease of Pro and a simultaneous increase of Glu serum level could be considered as a marker of acute IS.

Application of the feed additive «Abiotonic» in poultry farming

2021 ◽  
Vol 1 (10) ◽  
pp. 72-78
Author(s):  
Valentina M. Bachinskaya ◽  
◽  
Aleksandr A. Deltsov ◽  
Dmitrii V. Gonchar ◽  
◽  
...  
Keyword(s):  
Amino Acids ◽  
Egg Production ◽  
Laying Hens ◽  
Average Daily Gain ◽  
Live Weight ◽  
Poultry Meat ◽  
Feed Additive ◽  
Control Group ◽  
Standard Diet ◽  
Nonessential Amino Acids

This paper presents a study of the effect of the feed additive «Abiotonic» on the indicators of egg and meat productivity when applied to laying hens. It was found that with the additional introduction of this additive into the standard diet of poultry, the content of nonessential amino acids in their meat is 10% higher compared to the same parameters in the meat of chickens of the control group, the content of partially nonessential amino acids is by 8,5%, and of irreplaceable amino acids is by 6,7%. In addition, in the meat of laying hens, which used the feed additive «Abiotonic», the content of selenium (Se) is 29,4% higher than in the meat of birds of the control group, and iron (Fe) – by 20%. The average daily gain in live weight of the birds in the experimental group was 3,6 g more than in the control hens. Microbiological research made it possible to establish that poultry meat, regardless of the introduction of the feed additive «Abiotonik», meets the established requirements and is safe for human consumption. «Abiotonic», in addition, significantly improves the egg production of birds. It was found that the weight of eggs with the introduction of this feed additive in the diet in addition to the compound feed increased by an average of 1,2 g, and the egg production increased by 23 eggs in 7 days.

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