Faculty Opinions recommendation of Buffer AVL alone does not inactivate ebola virus in a representative clinical sample type.

2015 ◽  
Author(s):  
Raymond Lin
Keyword(s):  
Ebola Virus ◽  
Clinical Sample ◽  
Sample Type

scholarly journals Buffer AVL Alone Does Not Inactivate Ebola Virus in a Representative Clinical Sample Type

2015 ◽  
Vol 53 (10) ◽  
pp. 3148-3154 ◽  
Author(s):  
Sophie J. Smither ◽  
Simon A. Weller ◽  
Amanda Phelps ◽  
Lin Eastaugh ◽  
Sarah Ngugi ◽  
...  
Keyword(s):  
Ebola Virus ◽  
Clinical Sample ◽  
Rna Extraction ◽  
Heat Treatments ◽  
Clinical Samples ◽  
Enzyme Linked Immunosorbent Assay ◽  
Sample Type ◽  
Viral Inactivation ◽  
Blood Samples ◽  
Diagnostic Samples

Rapid inactivation of Ebola virus (EBOV) is crucial for high-throughput testing of clinical samples in low-resource, outbreak scenarios. The EBOV inactivation efficacy of Buffer AVL (Qiagen) was tested against marmoset serum (EBOV concentration of 1 × 10850% tissue culture infective dose per milliliter [TCID50· ml−1]) and murine blood (EBOV concentration of 1 × 107TCID50· ml−1) at 4:1 vol/vol buffer/sample ratios. Posttreatment cell culture and enzyme-linked immunosorbent assay (ELISA) analysis indicated that treatment with Buffer AVL did not inactivate EBOV in 67% of samples, indicating that Buffer AVL, which is designed for RNA extraction and not virus inactivation, cannot be guaranteed to inactivate EBOV in diagnostic samples. Murine blood samples treated with ethanol (4:1 [vol/vol] ethanol/sample) or heat (60°C for 15 min) also showed no viral inactivation in 67% or 100% of samples, respectively. However, combined Buffer AVL and ethanol or Buffer AVL and heat treatments showed total viral inactivation in 100% of samples tested. The Buffer AVL plus ethanol and Buffer AVL plus heat treatments were also shown not to affect the extraction of PCR quality RNA from EBOV-spiked murine blood samples.

scholarly journals Relationship between super antigenicity, antimicrobial resistance and origin of Staphylococcus aureus isolated

2016 ◽  
pp. 15-20 ◽  
Author(s):  
Jose Ignacio Moncayo ◽  
Jenna Samara Luligo Espinal ◽  
Jorge Javier Santacruz Ibarra ◽  
Adalucy Álvarez Aldana
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Antimicrobial Susceptibility ◽  
Clinical Sample ◽  
Clinical Specimen ◽  
Food Poisoning ◽  
Sample Type ◽  
Bacterial Populations ◽  
Specimen Type ◽  
Significant Difference

Introduction: Staphylococcus aureus is a pathogen that causes food poisoning as well as hospital and community acquired infections. Objective: Establish the profile of superantigen genes among hospital isolates in relation to clinical specimen type, susceptibility to antibiotics and hospital or community acquisition. Methods: Eighty one isolates obtained from patients at Colombian hospital, were classified by antimicrobial susceptibility, specimen type and hospital or community acquired . The PCR uniplex and multiplex was used for detection of 22 superantigen genes (18 enterotoxins, tsst-1 and three exfoliative toxins). Results: Ninety five point one percent of isolates harbored one or more of the genes with an average of 5.6 genes. Prevalence of individual genes was variable and the most prevalent was seg (51.9%). 39 genotypes were obtained, and the genotype gimnou (complete egc cluster) was the most prevalent alone (16.0%) and in association with other genes (13.6%). The correlation between presence of superantigens and clinical specimen or antimicrobial susceptibility showed no significant difference. But there was significant difference between presence of superantigens and the origin of the isolates, hospital or community acquired (p= 0.049). Conclusions: The results show the variability of the superantigen genes profile in hospital isolates and shows no conclusive relationship with the clinical sample type and antimicrobial susceptibility, but there was correlation with community and hospital isolates. The analysis of the interplay between virulence, epidemic and antibiotic resistance of bacterial populations is needed to predict the future of infectious diseases.

Taxometric Investigation of Depression: Evidence of Consistent Latent Structure Across Clinical and Community Samples

2007 ◽  
Vol 41 (5) ◽  
pp. 403-410 ◽  
Author(s):  
Tim Slade
Keyword(s):  
Anxiety Disorders ◽  
Clinical Sample ◽  
Community Sample ◽  
Research Data ◽  
Latent Structure ◽  
Data Sets ◽  
Sample Type ◽  
Maximum Eigenvalue ◽  
Taxometric Analysis ◽  
Taxometric Analyses

Objective: Past taxometric studies of depression have yielded equivocal results. Diversity of sample type may provide one explanation for this. The aim of the present study was to examine the latent structure of depression across clinical and community samples using exactly the same taxometric procedures involving exactly the same indicators of depression. Method: Two taxometric procedures, MAXEIG (maximum eigenvalue) and MAMBAC (mean above minus mean below a cut), were carried out on a clinical sample of 960 outpatients with mood and anxiety disorders. Simulated categorical and dimensional data sets as well as other consistency tests aided in the interpretation of the research data. Results were compared to a prior taxometric analysis in a community sample. Results: The results of the current taxometric analyses were consistent with a dimensional latent structure and were compatible with the findings from identical analyses in a community sample. Conclusions: The findings of the current study highlight the importance of identifying factors that may contribute to, and explain, differences in the identified latent structure of depression.

Adolescents’ self-mutilation - Relationship with dependent behaviour

2003 ◽  
Vol 62 (4) ◽  
pp. 241-249 ◽  
Author(s):  
M. Bolognini ◽  
B. Plancherel ◽  
J. Laget ◽  
P. Stéphan ◽  
O. Halfon
Keyword(s):  
Suicide Attempts ◽  
Clinical Sample ◽  
Control Sample ◽  
Clinical Population ◽  
Control Group ◽  
Clinical Group ◽  
Self Mutilation ◽  
Self Harm ◽  
Partial Overlap ◽  
The Relationship

The aim of this study, which was carried out in the French-speacking part of Switzerland, was to examine the relationship between suicide attempts and self-mutilation by adolescents and young adults. The population, aged 14-25 years (N = 308), included a clinical sample of dependent subjects (drug abuse and eating disorders) compared to a control sample. On the basis of the Mini Neuropsychiatric Interview ( Sheehan et al., 1998 ), DSM-IV criteria were used for the inclusion of the clinical population. The results concerning the occurrence of suicide attempts as well as on self-mutilation confirm most of the hypotheses postulated: suicidal attempts and self-mutilation were more common in the clinical group compared to the control group, and there was a correlation between suicide attempts and self-mutilation. However, there was only a partial overlap, attesting that suicide and self-harm might correspond to two different types of behaviour.

Test-Retest Reliability of a Self-Report Questionnaire for DSM-IV and ICD-10 Personality Disorders

2000 ◽  
Vol 16 (1) ◽  
pp. 53-58 ◽  
Author(s):  
Hans Ottosson ◽  
Martin Grann ◽  
Gunnar Kullgren
Keyword(s):  
Personality Disorder ◽  
Anxiety Disorders ◽  
Personality Disorders ◽  
Clinical Sample ◽  
Self Report ◽  
Anxiety State ◽  
Short Term ◽  
Retest Reliability ◽  
Axis I ◽  
Test Retest Reliability

Summary: Short-term stability or test-retest reliability of self-reported personality traits is likely to be biased if the respondent is affected by a depressive or anxiety state. However, in some studies, DSM-oriented self-reported instruments have proved to be reasonably stable in the short term, regardless of co-occurring depressive or anxiety disorders. In the present study, we examined the short-term test-retest reliability of a new self-report questionnaire for personality disorder diagnosis (DIP-Q) on a clinical sample of 30 individuals, having either a depressive, an anxiety, or no axis-I disorder. Test-retest scorings from subjects with depressive disorders were mostly unstable, with a significant change in fulfilled criteria between entry and retest for three out of ten personality disorders: borderline, avoidant and obsessive-compulsive personality disorder. Scorings from subjects with anxiety disorders were unstable only for cluster C and dependent personality disorder items. In the absence of co-morbid depressive or anxiety disorders, mean dimensional scores of DIP-Q showed no significant differences between entry and retest. Overall, the effect from state on trait scorings was moderate, and it is concluded that test-retest reliability for DIP-Q is acceptable.

Structuring Assessments of Psychopathology

2006 ◽  
Vol 27 (2) ◽  
pp. 87-92 ◽  
Author(s):  
Willem K.B. Hofstee ◽  
Dick P.H. Barelds ◽  
Jos M.F. Ten Berge
Keyword(s):  
Principal Components ◽  
Personality Assessment ◽  
Clinical Sample ◽  
Principal Component ◽  
Normal Sample ◽  
Normative Sample ◽  
Assessment Data ◽  
Obsessive Compulsive ◽  
Oblique Rotation ◽  
Two Samples

Hofstee and Ten Berge (2004a) have proposed a new look at personality assessment data, based on a bipolar proportional (-1, .. . 0, .. . +1) scale, a corresponding coefficient of raw-scores likeness L = ΢XY/N, and raw-scores principal component analysis. In a normal sample, the approach resulted in a structure dominated by a first principal component, according to which most people are faintly to mildly socially desirable. We hypothesized that a more differentiated structure would arise in a clinical sample. We analyzed the scores of 775 psychiatric clients on the 132 items of the Dutch Personality Questionnaire (NPV). In comparison to a normative sample (N = 3140), the eigenvalue for the first principal component appeared to be 1.7 times as small, indicating that such clients have less personality (social desirability) in common. Still, the match between the structures in the two samples was excellent after oblique rotation of the loadings. We applied the abridged m-dimensional circumplex design, by which persons are typed by their two highest scores on the principal components, to the scores on the first four principal components. We identified five types: Indignant (1-), Resilient (1-2+), Nervous (1-2-), Obsessive-Compulsive (1-3-), and Introverted (1-4-), covering 40% of the psychiatric sample. Some 26% of the individuals had negligible scores on all type vectors. We discuss the potential and the limitations of our approach in a clinical context.

Internal Structure of the Spanish Adaptation of the Eating Disorder Inventory-3

2012 ◽  
Vol 28 (1) ◽  
pp. 25-31 ◽  
Author(s):  
Paula Elosua ◽  
Alicia López-Jáuregui
Keyword(s):  
Eating Disorders ◽  
Eating Disorder ◽  
Internal Structure ◽  
Internal Consistency ◽  
Clinical Sample ◽  
Temporal Stability ◽  
Second Order ◽  
Order Structure ◽  
Eating Disorder Inventory ◽  
High Internal Consistency

In this study the Eating Disorder Inventory-3 was adapted to Spanish and analyzed the internal psychometric properties of the test in a clinical sample of females with eating disorders. The results showed a high internal consistency of the scores as well as high temporal stability. The factor structure of the scale composites was analyzed using confirmatory factor analysis. The results supported the existence of a second-order structure beyond the psychological composites. The second-order factor showed high correlation with the factor related to eating disorders. Overall, the Spanish version of the EDI-3 showed good psychometric qualities in terms of internal consistency, temporal stability and internal structure.

Measurement Invariance of the Parent Version of the Strengths and Difficulties Questionnaire (SDQ) Across Community and Clinical Populations

2018 ◽  
Vol 34 (4) ◽  
pp. 238-246 ◽  
Author(s):  
Iris A. M. Smits ◽  
Meinou H. C. Theunissen ◽  
Sijmen A. Reijneveld ◽  
Maaike H. Nauta ◽  
Marieke E. Timmerman
Keyword(s):  
Measurement Invariance ◽  
Clinical Sample ◽  
Community Sample ◽  
Strengths And Difficulties Questionnaire ◽  
Emotional And Behavioral Problems ◽  
Clinical Settings ◽  
Favorable Result ◽  
Strengths And Difficulties ◽  
Parent Version ◽  
Clinical Populations

Abstract. The Strengths and Difficulties Questionnaire (SDQ) is a popular screening instrument for the detection of social-emotional and behavioral problems in children in community and clinical settings. To sensibly compare SDQ scores across these settings, the SDQ should measure psychosocial difficulties and strengths in the same way across community and clinical populations, that is, the SDQ should be measurement invariant across both populations. We examined whether measurement invariance of the parent version of the SDQ holds using data from a community sample (N = 707) and a clinical sample (N = 931). The results of our analysis suggest that measurement invariance of the SDQ parent version across community and clinical populations is tenable, implying that one can compare the SDQ scores of children across these populations. This is a favorable result since it is common clinical practice to interpret the scores of a clinical individual relative to norm scores that are based on community samples. The findings of this study support the continued use of the parent version of the SDQ in community and clinical settings.

Are Three Items Sufficient to Measure Sense of Coherence?

2018 ◽  
Vol 34 (4) ◽  
pp. 229-237 ◽  
Author(s):  
Francesca Chiesi ◽  
Andrea Bonacchi ◽  
Caterina Primi ◽  
Alessandro Toccafondi ◽  
Guido Miccinesi
Keyword(s):  
Sense Of Coherence ◽  
Convergent Validity ◽  
Clinical Sample ◽  
Clinical Samples ◽  
Depression Symptoms ◽  
Time Saving ◽  
Positive Role ◽  
Patients With Cancer ◽  
Measure Sense

Abstract. The present study aimed at evaluating if the three-item sense of coherence (SOC) scale developed by Lundberg and Nystrom Peck (1995) can be effectively used for research purpose in both nonclinical and clinical samples. To provide evidence that it represents adequately the measured construct we tested its validity in a nonclinical (N = 658) and clinical sample (N = 764 patients with cancer). Results obtained in the nonclinical sample attested a positive relation of SOC – as measured by the three-item SOC scale – with Antonovsky’s 13-item and 29-item SOC scales (convergent validity), and with dispositional optimism, sense of mastery, anxiety, and depression symptoms (concurrent validity). Results obtained in the clinical sample confirmed the criterion validity of the scale attesting the positive role of SOC – as measured by the three-item SOC scale – on the person’s capacity to respond to illness and treatment. The current study provides evidence that the three-item SOC scale is a valid, low-loading, and time-saving instrument for research purposes on large sample.

The Beck Hopelessness Scale

2016 ◽  
Vol 32 (2) ◽  
pp. 111-118 ◽  
Author(s):  
Marianna Szabó ◽  
Veronika Mészáros ◽  
Judit Sallay ◽  
Gyöngyi Ajtay ◽  
Viktor Boross ◽  
...  
Keyword(s):  
Binary Data ◽  
Factor Model ◽  
Clinical Sample ◽  
Analytical Techniques ◽  
Cross Cultural ◽  
Cultural Validity ◽  
Beck Hopelessness Scale ◽  
Method Effects ◽  
Hungarian Population ◽  
Components Analysis

Abstract. The aim of the present study was to examine the construct and cross-cultural validity of the Beck Hopelessness Scale (BHS; Beck, Weissman, Lester, & Trexler, 1974 ). Beck et al. applied exploratory Principal Components Analysis and argued that the scale measured three specific components (affective, motivational, and cognitive). Subsequent studies identified one, two, three, or more factors, highlighting a lack of clarity regarding the scale’s construct validity. In a large clinical sample, we tested the original three-factor model and explored alternative models using both confirmatory and exploratory factor analytical techniques appropriate for analyzing binary data. In doing so, we investigated whether method variance needs to be taken into account in understanding the structure of the BHS. Our findings supported a bifactor model that explicitly included method effects. We concluded that the BHS measures a single underlying construct of hopelessness, and that an incorporation of method effects consolidates previous findings where positively and negatively worded items loaded on separate factors. Our study further contributes to establishing the cross-cultural validity of this instrument by showing that BHS scores differentiate between depressed, anxious, and nonclinical groups in a Hungarian population.

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