Phenotyping Tomato Root Developmental Plasticity in Response to Salinity in Soil Rhizotrons

Plants have developed multiple strategies to respond to salt stress. In order to identify new traits related to salt tolerance, with potential breeding application, the research focus has recently been shifted to include root system architecture (RSA) and root plasticity. Using a simple but effective root phenotyping system containing soil (rhizotrons), RSA of several tomato cultivars and their response to salinity was investigated. We observed a high level of root plasticity of tomato seedlings under salt stress. The general root architecture was substantially modified in response to salt, especially with respect to position of the lateral roots in the soil. At the soil surface, where salt accumulates, lateral root emergence was most strongly inhibited. Within the set of tomato cultivars, H1015 was the most tolerant to salinity in both developmental stages studied. A significant correlation between several root traits and aboveground growth parameters was observed, highlighting a possible role for regulation of both ion content and root architecture in salt stress resilience.

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Overexpression of the Auxin Receptor AFB3 in Arabidopsis Results in Salt Stress Resistance and the Modulation of NAC4 and SZF1

International Journal of Molecular Sciences ◽

10.3390/ijms21249528 ◽

2020 ◽

Vol 21 (24) ◽

pp. 9528

Author(s):

Fernanda Garrido-Vargas ◽

Tamara Godoy ◽

Ricardo Tejos ◽

José Antonio O’Brien

Keyword(s):

Salt Stress ◽

Stress Response ◽

Growth And Development ◽

Root Architecture ◽

Crop Production ◽

Lateral Roots ◽

Auxin Signaling ◽

Auxin Receptor ◽

Salt Stress Response ◽

Regulatory Pathways

Soil salinity is a key problem for crop production worldwide. High salt concentration in soil negatively modulates plant growth and development. In roots, salinity affects the growth and development of both primary and lateral roots. The phytohormone auxin regulates various developmental processes during the plant’s life cycle, including several aspects of root architecture. Auxin signaling involves the perception by specialized receptors which module several regulatory pathways. Despite their redundancy, previous studies have shown that their functions can also be context-specific depending on tissue, developmental or environmental cues. Here we show that the over-expression of Auxin Signaling F-Box 3 receptor results in an increased resistance to salinity in terms of root architecture and germination. We also studied possible downstream signaling components to further characterize the role of auxin in response to salt stress. We identify the transcription factor SZF1 as a key component in auxin-dependent salt stress response through the regulation of NAC4. These results give lights of an auxin-dependent mechanism that leads to the modulation of root system architecture in response to salt identifying a hormonal cascade important for stress response.

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Response of seven tomato (Lycopersicon esculentum Mill.) cultivars produced in Benin to salinity stress at young plant stage

International Journal of Current Research in Biosciences and Plant Biology ◽

10.20546/ijcrbp.2020.708.001 ◽

2020 ◽

Vol 7 (8) ◽

pp. 1-11

Author(s):

Eliane Kinsou ◽

Armel Mensah ◽

David K. Montcho Hambada ◽

Séraphin Ahissou Zanklan ◽

Agapit Wouyou ◽

...

Keyword(s):

Salt Stress ◽

Plant Growth ◽

Growth Parameters ◽

Growth Parameter ◽

Salt Resistance ◽

Young Plant ◽

Resistance Level ◽

Significant Difference ◽

Plant Stage ◽

Tomato Cultivars

In this study, we evaluated the salt resistance level of seven tomato cultivars Akikon, Tounvi, Thorgal, F1 Mongal, Padma, Petomech and TLCV15 at young plant stage. The experiment was laid out as a Randomized Complete Design (RCD) with four replications in a greenhouse. Three-week old plants from the seven cultivars were submitted in pots containing a mixture of potting soil and sand to five NaCl concentrations: 0; 30; 60; 90 and 120 mM NaCl corresponding respectively to an electric conductivity of 0.221; 3.827; 6.47; 10.56 and 14.02 dS.m-1 by irrigation every two days. Plant growth parameters were evaluated after two weeks. Salt effect caused a reduction of young plant growth whatever the growth parameter considered with a significant difference among cultivars. Growth of cultivars Padma, Akikon and Petomech was more affected by salt stress with two or three growth parameters significantly reduced at 30 mM NaCl whereas no growth parameter was significantly affected at less than 60 mM NaCl for cultivars Thorgal and Tounvi. Thus, cultivars Akikon, Petomech and Padma appeared as the most sensitive to salt stress. In contrast, cultivars Tounvi and Thorgal appeared as the most salt-resistant. Cultivars F1 Mongal and TLCV15 had intermediary behavior.

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A comprehensive RNA-Seq-based gene expression atlas of the summer squash (Cucurbita pepo) provides insights into fruit morphology and ripening mechanisms

BMC Genomics ◽

10.1186/s12864-021-07683-2 ◽

2021 ◽

Vol 22 (1) ◽

Author(s):

Aliki Xanthopoulou ◽

Javier Montero-Pau ◽

Belén Picó ◽

Panagiotis Boumpas ◽

Eleni Tsaliki ◽

...

Keyword(s):

Gene Expression ◽

Cucurbita Pepo ◽

Developmental Stages ◽

Lateral Roots ◽

Expression Patterns ◽

Male Flower ◽

Differentially Expressed ◽

Gene Expression Atlas ◽

Summer Squash ◽

Expression Atlas

Abstract Background Summer squash (Cucurbita pepo: Cucurbitaceae) are a popular horticultural crop for which there is insufficient genomic and transcriptomic information. Gene expression atlases are crucial for the identification of genes expressed in different tissues at various plant developmental stages. Here, we present the first comprehensive gene expression atlas for a summer squash cultivar, including transcripts obtained from seeds, shoots, leaf stem, young and developed leaves, male and female flowers, fruits of seven developmental stages, as well as primary and lateral roots. Results In total, 27,868 genes and 2352 novel transcripts were annotated from these 16 tissues, with over 18,000 genes common to all tissue groups. Of these, 3812 were identified as housekeeping genes, half of which assigned to known gene ontologies. Flowers, seeds, and young fruits had the largest number of specific genes, whilst intermediate-age fruits the fewest. There also were genes that were differentially expressed in the various tissues, the male flower being the tissue with the most differentially expressed genes in pair-wise comparisons with the remaining tissues, and the leaf stem the least. The largest expression change during fruit development was early on, from female flower to fruit two days after pollination. A weighted correlation network analysis performed on the global gene expression dataset assigned 25,413 genes to 24 coexpression groups, and some of these groups exhibited strong tissue specificity. Conclusions These findings enrich our understanding about the transcriptomic events associated with summer squash development and ripening. This comprehensive gene expression atlas is expected not only to provide a global view of gene expression patterns in all major tissues in C. pepo but to also serve as a valuable resource for functional genomics and gene discovery in Cucurbitaceae.

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Effect of Salt Stress on Growth and Metabolite Profiles of Cape Gooseberry (Physalis peruviana L.) along Three Growth Stages

Molecules ◽

10.3390/molecules26092756 ◽

2021 ◽

Vol 26 (9) ◽

pp. 2756

Author(s):

Daissy Monroy-Velandia ◽

Ericsson Coy-Barrera

Keyword(s):

Salt Stress ◽

Growth Parameters ◽

Fruit Production ◽

Growth Stages ◽

Salt Stress Response ◽

Physalis Peruviana ◽

Metabolite Profiles ◽

Consumption Practices ◽

Growth Differences ◽

Cape Gooseberry

Colombia is the main producer of cape gooseberry (Physalis peruviana L.), a plant known for its various consumption practices and medicinal properties. This plant is generally grown in eroded soils and is considered moderately tolerant to unfavorable conditions, such as nutrient-poor soils or high salt concentrations. Most studies conducted on this plant focus on fruit production and composition because it is the target product, but a small number of studies have been conducted to describe the effect of abiotic stress, e.g., salt stress, on growth and biochemical responses. In order to better understand the mechanism of inherent tolerance of this plant facing salt stress, the present study was conducted to determine the metabolic and growth differences of P. peruviana plants at three different BBCH-based growth substages, varying salt conditions. Hence, plants were independently treated with two NaCl solutions, and growth parameters and LC-ESI-MS-derived semi-quantitative levels of metabolites were then measured and compared between salt treatments per growth substage. A 90 mM NaCl treatment caused the greatest effect on plants, provoking low growth and particular metabolite variations. The treatment discrimination-driving feature classification suggested that glycosylated flavonols increased under 30 mM NaCl at 209 substages, withanolides decreased under 90 mM NaCl at 603 and 703 substages, and up-regulation of a free flavonol at all selected stages can be considered a salt stress response. Findings locate such response into a metabolic context and afford some insights into the plant response associated with antioxidant compound up-regulation.

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Supplemental Selenium and Boron Mitigate Salt-Induced Oxidative Damages in Glycine max L.

Plants ◽

10.3390/plants10102224 ◽

2021 ◽

Vol 10 (10) ◽

pp. 2224

Author(s):

Mira Rahman ◽

Khussboo Rahman ◽

Khadeja Sultana Sathi ◽

Md. Mahabub Alam ◽

Kamrun Nahar ◽

...

Keyword(s):

Oxidative Stress ◽

Salt Stress ◽

Antioxidant Defense ◽

Foliar Application ◽

Growth Parameters ◽

Monodehydroascorbate Reductase ◽

Ros Scavenging ◽

H2o2 Content ◽

Mda Content ◽

Different Levels

The present investigation was executed with an aim to evaluate the role of exogenous selenium (Se) and boron (B) in mitigating different levels of salt stress by enhancing the reactive oxygen species (ROS) scavenging, antioxidant defense and glyoxalase systems in soybean. Plants were treated with 0, 150, 300 and 450 mM NaCl at 20 days after sowing (DAS). Foliar application of Se (50 µM Na2SeO4) and B (1 mM H3BO3) was accomplished individually and in combined (Se+B) at three-day intervals, at 16, 20, 24 and 28 DAS under non-saline and saline conditions. Salt stress adversely affected the growth parameters. In salt-treated plants, proline content and oxidative stress indicators such as malondialdehyde (MDA) content and hydrogen peroxide (H2O2) content were increased with the increment of salt concentration but the relative water content decreased. Due to salt stress catalase (CAT), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), glyoxalase I (Gly I) and glyoxalase II (Gly II) activity decreased. However, the activity of ascorbate peroxidase (APX), glutathione reductase (GR), glutathione peroxidase (GPX), glutathione S-transferase (GST) and peroxidase (POD) increased under salt stress. On the contrary, supplementation of Se, B and Se+B enhanced the activities of APX, MDHAR, DHAR, GR, CAT, GPX, GST, POD, Gly I and Gly II which consequently diminished the H2O2 content and MDA content under salt stress, and also improved the growth parameters. The results reflected that exogenous Se, B and Se+B enhanced the enzymatic activity of the antioxidant defense system as well as the glyoxalase systems under different levels of salt stress, ultimately alleviated the salt-induced oxidative stress, among them Se+B was more effective than a single treatment.

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Mitigation of salt stress in rice plant at germination stage by using methyl jasmonate

Asian Journal of Medical and Biological Research ◽

10.3329/ajmbr.v2i1.27572 ◽

2016 ◽

Vol 2 (1) ◽

pp. 74-81 ◽

Cited By ~ 2

Author(s):

Sakil Mahmud ◽

Shayla Sharmin ◽

Bishan Lal Das Chowdhury ◽

Mohammad Anowar Hossain ◽

Muhammad Javidul Haque Bhuiyan

Keyword(s):

Salt Stress ◽

Methyl Jasmonate ◽

Root Length ◽

Growth Parameters ◽

Dry Weight ◽

Germination Percentage ◽

Alpha Amylase ◽

Rice Varieties ◽

Biochemical Component ◽

Germination Stage

To explore the possibility of using methyl jasmonate (MeJA) for alleviation of salt stress, the present study was conducted where six rice varieties (BRRI dhan31, BRRI dhan46, Gota, Kajalsail, Pokkali and Pengek) were grown in non saline (0 dSm-1), saline (12 dSm-1), saline (12 dSm-1) + 10 ?M MeJA and saline (12 dSm-1) + 20 ?M MeJA conditions at germination stage (till the 9 days). MeJA was applied by imbibing seeds in it for 24 hours. To evaluate the effect of MeJA on saline stressed plant at germination stage, change in growth parameters namely germination percentage, shoot and root length, fresh and dry wt. of shoot, fresh and dry wt. of root and biochemical component- activity of alpha-amylase were monitored. Salinity had a minimum effect on final germination percentage (FGP) of rice varieties, but delayed in attaining it. MeJA had least positive effect. Salinity significantly reduced the shoot and root length, fresh and dry weight of all the varieties. MeJA had more decreasing effect in susceptible varieties while increasing effect in moderately tolerant and tolerant. Activity of alpha-amylase in germinated seed varied from 0.051 mg to 0.111 mg mal./mg tissue. At 12dSm-1 salinity, susceptible and moderately tolerant varieties showed significant reduction but tolerant showed significant increase. MeJA intensified the negative effect further in susceptible varieties. In tolerant and moderately tolerant varieties, increment in activity took place and 10 ?M had a better effect over 20 ?M MeJA.Asian J. Med. Biol. Res. March 2016, 2(1): 74-81

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Effect of salt stress on some sweet corn (Zea mays L. var. saccharata) genotypes

Archives of Biological Sciences ◽

10.2298/abs150121062s ◽

2015 ◽

Vol 67 (3) ◽

pp. 993-1000 ◽

Cited By ~ 3

Author(s):

Lydia Shtereva ◽

Roumiana Vassilevska-Ivanova ◽

Tanya Karceva

Keyword(s):

Zea Mays ◽

Salt Stress ◽

Zea Mays L ◽

Sweet Corn ◽

Growth Parameters ◽

Dry Weight ◽

Germination Percentage ◽

F1 Hybrid ◽

Salt Stress Condition ◽

Salinity Levels

An experiment was carried out hydroponically under laboratory conditions to investigate the effect of salt stress on several physiological and biochemical parameters of three sweet corn (Zea mays L. var. saccharata) genotypes: lines 6-13, C-6 (pollen source) and their heterotic F1 hybrid ?Zaharina?. The degree of salinity tolerance among these genotypes was evaluated at three different sodium chloride (NaCl) concentrations: 0 mM, 100 mM, 125 mM and 150 mM. Seed germination, plant growth and biochemical stress determining parameters such as malondialdehyde (MDA), proline content and hydrogen peroxide (H2O2) levels were compared between seedlings of lines and hybrid. The obtained results indicated that both lines and hybrid have similar responses at different salinity levels for all examined traits. All the seedlings? growth parameters, such as germination percentage, root length, shoot length, root and shoot fresh and dry weight, decreased with increasing salinity level. MDA, proline and H2O2 increased at different saline conditions in comparison to the control. Based on the results, of the three genotypes examined, the hybrid Zaharina, followed by line C-6, was more salt-sensitive than line 6-13 in salt stress condition.

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Influence of salt stress on plants of poplar clone "INRA 353-38" and willow clone "Zhytomyrska-1" in in vitro culture

Bulletin of Taras Shevchenko National University of Kyiv Series Biology ◽

10.17721/1728_2748.2020.83.43-49 ◽

2020 ◽

Vol 83 (4) ◽

pp. 43-49

Author(s):

Yu. Khoma ◽

L. Khudolieieva ◽

N. Kutsokon

Keyword(s):

Salt Stress ◽

Sodium Chloride ◽

In Vitro Culture ◽

Growth Parameters ◽

Hybrid Poplar ◽

Salt Resistance ◽

Soil Salinization ◽

In Vitro Cultivation ◽

Abiotic Factor

Soil salinization is an important abiotic factor negatively affecting plant growth, development and productivity. Fast-growing poplar and willow trees are important plants for bioenergy production demonstrating varying degrees of adaptation to different habitats. The study of salt resistance in different clones of poplars and willows will reveal genotypes that can be planted in saline soils for producing biomass for the bioenergy industry. Therefore, the aim of the study was to investigate the effects of salt stress on poplar plants of clone 'INRA 353-38' (Populus tremula × P. tremuloides) and willow clone 'Zhytomyrska – 1' (Salix sp.) under in vitro culture. For this purpose the plants were cultivated on MS nutrient medium with the addition of sodium chloride in concentrations 25 mM, 50 mM and 100 mM. The control plants were grown on the sodium chloridefree medium. The plant status (with a 4-score scale), the intensity of their growth (by shoot length) and rooting capacity (by the number of roots) were assessed on the 10th and the 30th day of cultivation. The results obtained indicate a high level of sensitivity to sodium chloride of both studied clones under in vitro cultivation. But the willow 'Zhytomyrska – 1' had a higher sensitivity to salt stress comparing to hybrid polar 'ІNRA 353-38' since growth parameters of willow were significantly decreased even under the concentration of sodium chloride 50 mM, and in the case of short term influence (10 days) of the highest concentration of sodium chloride (100 mM) all willow plants terminated their growth and quickly died. The growth parameters of hybrid poplar were declined within a month, mainly under the highest concentration of sodium chloride, but even under such conditions some part of the shoots were able to survive.

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Bare Root Liner Production Can Alter Tree Root Architecture

Journal of Environmental Horticulture ◽

10.24266/0738-2898-27.2.99 ◽

2009 ◽

Vol 27 (2) ◽

pp. 99-104 ◽

Cited By ~ 3

Author(s):

Angela Hewitt ◽

Gary Watson

Keyword(s):

Acer Saccharum ◽

Root Architecture ◽

Lateral Roots ◽

Primary Root ◽

Root Pruning ◽

Rooted Cuttings ◽

Nursery Production ◽

Structural Roots ◽

Production Practices ◽

Bare Root

Abstract Typical nursery production practices, such as root pruning and transplanting, can alter tree root architecture and contribute to root systems that are too deep. In a study of field-grown liner production, root architecture was examined at each stage of the production process, from first year seedlings or rooted cuttings, through 4 to 5 year old branched liners. Depth and diameter of structural roots were recorded on ten replications each of Acer saccharum, Gleditsia triancanthos, Pyrus calleryana, and apple seedling rootstocks; Platanus ‘Columbia’ clonal rooted cuttings; and apple EMLA 111 clonal rootstock produced by mound propagation. By the time the liners reached marketable size, most natural lateral roots emerging from the primary root were lost. Simultaneously, adventitious roots were produced deeper on the root shank at the pruned end of the primary root. These changes in architecture result in the formation of an ‘adventitious root flare’ that is deeper in the soil than a natural root flare. The depth of this new root flare is dependent upon nursery production practices and may influence the ultimate depth of structural roots in the landscape.

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EVALUATION OF Psidium guajava LEAVES AND ACETIC ACID AS NATURAL HERBICIDES FOR CONTROLLING SOME WEEDS OF Capsicum annuum CROP

JOURNAL OF WEED SCIENCE RESEARCH ◽

10.28941/pjwsr.v26i1.816 ◽

2020 ◽

Vol 27 (2) ◽

pp. 103-117

Author(s):

Mona Adel El-Wakeel

Keyword(s):

Acetic Acid ◽

Capsicum Annuum ◽

Growth Parameters ◽

Soil Surface ◽

Psidium Guajava ◽

Growth And Yield ◽

Allelopathic Effect ◽

Yield Traits ◽

Acetic Acid Treatment ◽

Leaf Powder

Allelopathy and natural products are safe non-chemical modern techniques that applied as alternative to synthetic herbicides for controlling weeds. So, two pot experiments were conducted to evaluate the allelopathic effect of Psidium guajava leaf powder (PLP) and Acetic acid 5% as a natural product on the growth and yield of Capsicum annuum plants and both associated weeds: Phalaris minor (grassy weed) and Malva parviflora (broad-leaved weed). PLP was mixed with in the soil surface at successive rates (15, 30, 45 and 60 g/pot). In the corresponding treatments PLP at the same sequenced rates was mixed with the soil then sprayed with acetic acid 5% immediately. Moreover, sole spraying of acetic acid 5% treatment was sprayed on the soil surface. All treatments were applied before transplanting directly. Results revealed that the maximum inhibition of both weeds in both seasons was recorded by PLP at 60g + Acetic acid 5% as compared to unweeded control. Concerning to C. annuum growth parameters and yield traits, sole application of PLP at successive rates is more effective than PLP at the same successive rates with acetic acid 5%. So, it was observed that PLP at 60g/pot and 45 g/pot significantly developed most of growth parameters and yield traits of C. annuum than the healthy plants in both seasons. On the contrary, acetic acid treatment alone recorded the lowest value of all growth parameters and yield traits of Capsicum annuum plants.Allelopathy and natural products are safe non-chemical modern techniques that applied as alternative to synthetic herbicides for controlling weeds. So, two pot experiments were conducted to evaluate the allelopathic effect of Psidium guajava leaf powder (PLP) and Acetic acid 5% as a natural product on the growth and yield of Capsicum annuum plants and both associated weeds: Phalaris minor (grassy weed) and Malva parviflora (broad-leaved weed). PLP was mixed with in the soil surface at successive rates (15, 30, 45 and 60 g/pot). In the corresponding treatments PLP at the same sequenced rates was mixed with the soil then sprayed with acetic acid 5% immediately. Moreover, sole spraying of acetic acid 5% treatment was sprayed on the soil surface. All treatments were applied before transplanting directly. Results revealed that the maximum inhibition of both weeds in both seasons was recorded by PLP at 60g + Acetic acid 5% as compared to unweeded control. Concerning to C. annuum growth parameters and yield traits, sole application of PLP at successive rates is more effective than PLP at the same successive rates with acetic acid 5%. So, it was observed that PLP at 60g/pot and 45 g/pot significantly developed most of growth parameters and yield traits of C. annuum than the healthy plants in both seasons. On the contrary, acetic acid treatment alone recorded the lowest value of all growth parameters and yield traits of Capsicum annuum plants.

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