scholarly journals The Photobiomodulation Effect of 940nm Laser Irradiation on Enterococcus faecalis in Human Root Dentin Slices of Varying Thicknesses

2021 ◽  
Vol 12 (1) ◽  
pp. e48-e48
Author(s):  
Bryan Martin Sher ◽  
Riaan Mulder ◽  
Norbert Gutknecht
Keyword(s):  
Laser Irradiation ◽  
Enterococcus Faecalis ◽  
Continuous Wave ◽  
Laser Energy ◽  
Positive Control ◽  
Significance Level ◽  
Colony Forming Units ◽  
Root Dentin ◽  
Post Hoc ◽  
One Way Anova

Introduction: An increase in dentine thickness could result in an inadequate depth of laser energy penetration. This study aimed to evaluate the effect of a 940 nm laser on Enterococcus faecalis through varying thicknesses of human root dentin slices. Methods: Thirty-five dentin slices of root dentin with thicknesses ranging between 500 and 3000 µm were produced. Six experimental groups (500, 1000, 1500, 2000, 2500, and 3000 µm (n=5 each) were lased and the seventh, non-lased group served as the positive control with a dentine thickness of 2000 µm. The slices were inoculated with 2 µL of E. faecalis suspension of 1.5 × 108 E. faecalis cells/mL. All the lased slices were lased from the opposing side of the inoculation. A non-initiated 200 μm bare end fibre at the power of 1 W, in a continuous wave was used. Four doses of laser irradiation of 5 seconds with a side to side movement with the tip held at a 5º angle to the dentine slice were performed. The colony-forming units of E. faecalis were determined and the bacterial photobiomodulation effect analysed using one-way ANOVA with a Bonferroni and Holm post hoc test at a significance level of P>0.05. Results: There were statistical differences between the dentin slices of 500, 1000, and 1500 μm treated with the laser compared to the positive control (P<0.01). However, there were no statistical differences between the lased 2000 and 2500 μm slices compared to the positive control. There was significantly more photobiomodulation of the E. faecalis for the dentine slices of 3000 μm than the positive control (P<0.01). Conclusion: Laser treatment through dentine slices of 2000 μm and thinner significantly reduced bacterial growth. The photobiomodulation effects started to occur in dentine slices thicker than 2500 μm compared to the positive control.

scholarly journals Influence of Temperature on the Antibacterial Activity of Sodium Hypochlorite

2016 ◽  
Vol 27 (1) ◽  
pp. 32-36 ◽  
Author(s):  
Luciano Giardino ◽  
Zahed Mohammadi ◽  
Riccardo Beltrami ◽  
Claudio Poggio ◽  
Carlos Estrela ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Brain Heart Infusion ◽  
Positive Control ◽  
Negative Control ◽  
Descriptive Statistics ◽  
Significance Level ◽  
Tukey Test ◽  
Colony Forming Units ◽  
Influence Of Temperature On ◽  
Root Dentin

Abstract The aim of this study was to compare the antimicrobial activity of 5.25% NaOCl, Hypoclean and Chlor-Xtra at 20 °C and 45 °C in bovine root dentin. One-hundred-and-seventy dentin tubes prepared from bovine maxillary incisors were infected for 21 days with Enterococcus faecalis. The specimens were divided into the following groups: 1. 5.25% NaOCl 20 °C; 2. Hypoclean 20 °C; 3. Chlor-Xtra 20 °C; 4. 5.25% % NaOCl 45 °C; 5. Hypoclean 45 °C; 6. Chlor-Xtra 45 °C; 7. positive control; 8. negative control. Dentin chips were collected with round burs into Brain Heart Infusion (BHI) broth. After culturing, the number of colony-forming units (CFU) was counted. Statistical analyses were performed using descriptive statistics (mean, standard deviation, median), Shapiro-Wilk test, ANOVA and Tukey test. Significance level was set at p<0.05. In all experimental groups, CFU was minimum after treatment (day 0) and the obtained results were significantly different from each other at any period (p<0.05). After treatment, the Hypoclean and Chlor-Xtra showed the lowest numbers of CFU at 20 °C and 45 °C, whereas 5.25% NaOCl showed the highest number of CFU at both temperatures. In each group, the number of CFUs increased significantly with time (p<0.05). The antibacterial activity of Hypoclean and Chlor-Xtra at 45 °C were significantly greater than other tested solutions.

scholarly journals Antibacterial Power of Sodium Hypochlorite Combined with Surfactants and Acetic Acid

2014 ◽  
Vol 25 (4) ◽  
pp. 289-294 ◽  
Author(s):  
Luciano Giardino ◽  
Carlos Estrela ◽  
Zahed Mohammadi ◽  
Flavio Palazzi
Keyword(s):  
Acetic Acid ◽  
Ex Vivo ◽  
Multiple Comparisons ◽  
Positive Control ◽  
Negative Control ◽  
Parametric Methods ◽  
Colony Forming Units ◽  
Root Dentin ◽  
Ex Vivo Study ◽  
One Way Anova

The aim of this ex vivo study was to compare the antibacterial power of 1% NaOCl with 1% acetic acid, 5.25% NaOCl and two commercially available NaOCl modified with surfactants in bovine root dentin. A total of 120 dentin tubes prepared from intact bovine incisors were infected for 21 days with Enterococcus faecalis and randomly divided into six groups as follows: 5.25%NaOCl; Hypoclean; Chlor-Xtra; 1% NaOCl with 1% acetic acid; infected dentin tubes (positive control); and sterile dentin tubes (negative control). At experimental times of 0, 7, 14, 21 and 28 days, dentin chips were collected using sequential round burs with increasing diameters in separate test tubes containing 3 mL of freshly prepared BHI. Statistical analysis were performed using parametric methods (one-way ANOVA, and Bonferroni's multiple comparisons test, α=0.01). After culturing, the number of colony-forming units (CFU) was counted. All the NaOCl solutions showed small number of CFU over 28 days. ChlorXtra and Hypoclean had the smallest number of CFU at all times with greater antimicrobial efficacy than 5.25% NaOCl and 1% NaOCl solution with 1% acetic acid.

scholarly journals Antibacterial Efficacy of Synthetic and Natural-Derived Novel Endodontic Irrigant Solutions

2018 ◽  
Vol 29 (5) ◽  
pp. 459-464 ◽  
Author(s):  
Larissa Tais Soligo ◽  
Ediléia Lodi ◽  
Ana Paula Farina ◽  
Matheus Albino Souza ◽  
Cristina de Mattos Pimenta Vidal ◽  
...  
Keyword(s):  
Sodium Chloride ◽  
Enterococcus Faecalis ◽  
Grape Seed ◽  
Control Group ◽  
Bacterial Reduction ◽  
Calcium Hypochlorite ◽  
Root Canals ◽  
Colony Forming Units ◽  
Before And After ◽  
Post Hoc

Abstract The aim of this study was to compare the efficacy of grape seed extract (GSE), calcium hypochlorite [Ca(ClO)2], and sodium hypochlorite (NaOCl) irrigant solutions with rotary or reciprocating instrumentation for disinfection of root canals inoculated with Enterococcus faecalis. The mesiobuccal root canals of mandibular molars were prepared and inoculated with Enterococcus faecalis for 21 days. The roots were then randomly divided into the following eight experimental groups (n=11) according to the instrumentation technique and disinfection protocol: ProTaper Next or Reciproc R25 with sodium chloride (control group), 6% NaOCl, 6% Ca(ClO)2, or 50% GSE used for irrigation during instrumentation. The antimicrobial activity was determined on the basis of a reduction in colony-forming units (CFUs) counted on bacterial samples collected before and after root canal instrumentation and expressed as a percentage of reduction. Data were evaluated by two-way ANOVA followed by Tukey HSD post-hoc tests (p<0.05). No significant differences were observed in bacterial reduction between the ProTaper Next and Reciproc R25 systems (p>0.05), regardless of the irrigant solution used. Furthermore, all active solutions (6% NaOCl, 50% GSE, and 6% Ca(ClO)2) showed similar potential to reduce bacterial counts (p>0.05) and were significantly more effective than sodium chloride (control) (p<0.05). The results suggest that the GSE and Ca(ClO)2 have potential clinical application as irrigant solutions in endodontic therapy since they present bactericidal efficacy against Enterococcus faecalis.

scholarly journals Selected Goal – Scoring Characteristics in the National Hockey League

2021 ◽  
Vol 61 (2) ◽  
pp. 228-237
Author(s):  
Silvio Parničan ◽  
Pavol Peráček ◽  
Igor Tóth
Keyword(s):  
Ice Hockey ◽  
National Hockey League ◽  
Training Process ◽  
Significance Level ◽  
Game Situation ◽  
Post Hoc ◽  
Number Of Passes ◽  
Determining Factor ◽  
One Way Anova ◽  
Location Zone

Summary The variables impacting the efficiency of the offensive phase of the game in ice hockey may be determining factor for both the training process and game strategies. The research aimed to acquire and expand the knowledge about selected goal-scoring characteristics in the National Hockey League. A total of 511 even-strength goals were recorded in 129 randomly selected games in a regular-season 2020/2021 by indirect observation. Goals were differentiated by selected variable dimensions: location of the offensive team’s puck possession gain before scoring a goal, game situation preceding a goal, and the number of passes of the offensive team preceding a goal. Data were analyzed by performing a one–way ANOVA. Post hoc multiple comparisons were performed using the Tukey HSD test. The significance level of p ≤ 0.05 was used. Significant differences were found within all variable dimensions (p= 0.00001). In terms of the puck possession gain location, zone 1 (along the boards in the offensive zone) was the zone with the significantly highest mean score (p= 0.00305). Offensive zone play was significantly the game situation preceding most of the goals (p= 0.00000). The interval of the passes that preceded most of the recorded goals was the interval of 1 – 2 passes (p= 0.00000). Acquired results point out the dominance of particular variables and may help coaches to determine the content of the training process and game strategies. Based on the assumption of the realization of similar research in different environments in terms of the quality and age category, if the analogy would be found between the results of the present study and the results of potential studies, we can state the trends applicable to youth training.

scholarly journals EFEK ANTIBAKTERI EKSTRAK DAUN MIMBA (Azadirachta indica) TERHADAP PERTUMBUHAN Enterococcus faecalis SECARA IN-VITRO

2019 ◽  
Vol 11 (1) ◽  
pp. 23-32
Author(s):  
Cut Soraya ◽  
Sunnati ◽  
Fenny Wulandari
Keyword(s):  
Phenolic Compound ◽  
Enterococcus Faecalis ◽  
Azadirachta Indica ◽  
Control Group ◽  
Post Hoc ◽  
One Way Anova ◽  
Banda Aceh

Perawatan saluran akar adalah perawatan yang dilakukan untuk mempertahankan gigi agar dapatberfungsi di lengkung gigi selama mungkin. Penyebab dominan dari kegagalan perawatan endodontikadalah adanya bakteri di dalam saluran akar. Bakteri yang paling sering ditemukan pada isolasi darigigi yang mengalami kegagalan perawatan saluran akar adalah Enterococcus faecalis (E.faecalis).Salah satu jenis tanaman herbal yang memiliki sifat antibakteri yang tinggi adalah tanaman mimba(A.indica). Tujuan penelitian ini untuk menganalisis efek antibakteri ekstrak daun mimba (A.indica)terhadap pertumbuhan E. faecalis. Penelitian ini merupakan penelitian eksperimental laboratorisdengan desain penelitian true experimental posttest only control group. Penelitian dilalukan denganmenguji ekstrak daun mimba (A.indica) pada konsentrasi 10%, 20%, 40%, 60%, 80% dan 100%terhadap pertumbuhan E. faecalis yang dilakukan di Laboratorium Mikrobiologi Fakultas KedokteranHewan (FKH) Universitas Syiah Kuala Banda Aceh. Hasil uji fitokimia yang dilakukan menunjukkanbahwa ekstrak daun mimba (A. indica) positif mengandung triterpenoid, phenolic compound, tanin,steroid dan saponin yang berperan sebagai antibakteri. Hasil uji efek antibakteri ekstrak daun mimba(A. indica) menunjukkan adanya pembentukan zona hambat di sekitar kertas cakram pada setiapkonsentrasi. Hal ini membuktikan bahwa ekstrak daun mimba memiliki efek antibakteri yangmemiliki kemampuan dalam menghambat pertumbuhan E. faecalis. Uji statistik digunakanmenggunakan one way ANOVA. Kemudian dilakukan uji lanjut (Post hoc test) menggunakan LeastSignificant Difference untuk menganalisis perbedaan efek antibakteri ekstrak daun mimba (A. indica)dalam konsentrasi 10%, 20%, 40%, 60%, 80% dan 100% terhadap pertumbuhan E.faecalis.Kesimpulan penelitian ini ekstrak daun mimba (A. indica) berpotensi menghambatpertumbuhan E. faecalis.Kata Kunci: Azadirachta indica, Enterococcus faecalis, perawatan saluran akar.

scholarly journals Assessment of apical microleakage using tooth clearing method between two softened core obturation techniques

2016 ◽  
Vol 1 (1) ◽  
pp. 39
Author(s):  
Nadia S. Wongsari ◽  
Christine A. Rovani ◽  
Aries C. Trilaksana
Keyword(s):  
Root Canal ◽  
Continuous Wave ◽  
Positive Control ◽  
Single Root ◽  
The Third ◽  
Gutta Percha ◽  
Nail Polish ◽  
Wave Technique ◽  
Post Hoc ◽  
Clearing Method

This study aimed to compare apical microleakage using tooth clearing method between two softened core obturation techniques which are carrier based gutta percha and continuous wave. Samples are maxillary first incisors, with a single root canal. They are devided into three groups of 9 each randomly. The first group is carrier based gutta percha, the second is continuous wave, and the third is lateral condensation as positive control. The teeth were decoronated at the cementoenamel junction, prepared with crown down pressureless technique, and obturated according their group.  All the samples were coated with nail polish except for 2 mm from the apical, and then placed in China Ink for 7 days. After that, the polish were removed and the samples undergone tooth clearing procedure. When the samples had been transparant, they were observed using microscop. Data were collected and analyzed using Anova and Tukey’s post hoc test. The study showed that mean of apical microleakage from continuous wave technique (0.256+0.133) is less than from carrier based gutta percha (0.433+0.173). As the conclusion, there is a significant diffrence (p:0.034) of apical microleakage between continuous wave and carrier based gutta percha (p<0.05).

scholarly journals MORPHOLOGICAL ADJUSTMENTS OF THE RADIAL NERVE ARE INTENSITY-DEPENDENT

2017 ◽  
Vol 23 (1) ◽  
pp. 55-59 ◽  
Author(s):  
Patrícia Oliva Carbone ◽  
Walter Krause Neto ◽  
Eliane Florencio Gama ◽  
Wellington de Assis Silva ◽  
Thatiana Lacerda Nobre ◽  
...  
Keyword(s):  
Radial Nerve ◽  
Morphological Analysis ◽  
Morphological Changes ◽  
The Other ◽  
Significance Level ◽  
Training Groups ◽  
Transmission Electron ◽  
Post Hoc ◽  
And Training ◽  
One Way Anova

ABSTRACT Introduction: Peripheral nerve adaptation is critical for strength gains. However, information about intensity effects on nerve morphology is scarce. Objective: To compare the effects of different intensities of resistance training on radial nerve structures. Methods: Rats were divided into three groups: control (GC), training with 50% (GF1) and training 75% (GF2) of the animal’s body weight. The morphological analysis of the nerve was done by light and transmission electron microscopy. One-way ANOVA and the Tukey’s post hoc test were applied and the significance level was set at p≤0.05. Results: Training groups had an increase of strength compared to GC (p≤0.05). All measured nerve components (mean area and diameter of myelin fibers and axons, mean area and thickness of the myelin sheath, and of neurofilaments and microtubules) were higher in GF2 compared to the other (p≤0.05). Conclusion: Results demonstrated greater morphological changes on radial nerve after heavier loads. This can be important for rehabilitation therapies, training, and progression.

scholarly journals Effect of Nanosilver Gel, Chlorhexidine Gluconate, and Camphorated Phenol on Enterococcus faecalis Biofilm

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Dong Bo ◽  
Cecilia Marcellino Kayombo
Keyword(s):  
Enterococcus Faecalis ◽  
Laser Scanning ◽  
Statistical Significance ◽  
Chlorhexidine Gluconate ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Control Analysis ◽  
Significant Difference ◽  
Post Hoc ◽  
One Way Anova

Aim. To assess the effectiveness of nanosilver gel (NSG) in comparison to chlorhexidine gluconate (CHX) and camphorated phenol (CP) against Enterococcus faecalis (E.f) biofilm. Methods and Materials. Two tests were done, methyl thiazolyl tetrazolium (MTT) assay and confocal laser scanning microscopy (CLSM) analysis, to determine the effectiveness of NSG, CHX, and CP on E.f biofilm. Polystyrene microtiter 96- and 6-well plates were used for MTT and CLSM, respectively. Nanosilver gel was in three concentrations (0.05%, 0.1%, and 0.2%), chlorhexidine gluconate used was 2%, and camphorated phenol and normal saline were as control. Analysis was done using one-way ANOVA; the post hoc test was run for multiple comparisons. The level of statistical significance was set at P<0.05. Results. One-way ANOVA showed significant differences among groups (0.05% NSG and CP, 0.1% NSG and CP, 0.2% NSG and CP, 0.1% NSG and 2% CHX, 0.2% and NSG and 2% CHX) (P<0.001) and also showed significant difference between groups (P<0.001), f-ratio 87.823. A post hoc Tukey’s test revealed no significant difference between chlorhexidine gluconate and 0.05% nanosilver gel (P>0.05). Conclusions. 0.1% and 0.2% nanosilver gel is more effective on Enterococcus faecalis biofilm as compared to chlorhexidine gluconate and camphorated phenol.

scholarly journals Effect of Hydrogen Peroxide on the Antibacterial Substantivity of Chlorhexidine

2010 ◽  
Vol 2010 ◽  
pp. 1-4 ◽  
Author(s):  
Shahriar Shahriari ◽  
Zahed Mohammadi ◽  
Mohammadi Mehdi Mokhtari ◽  
Rasoul Yousefi
Keyword(s):  
Hydrogen Peroxide ◽  
Antibacterial Activity ◽  
Enterococcus Faecalis ◽  
Time Lapse ◽  
Positive Control ◽  
Negative Control ◽  
The Other ◽  
Time Period ◽  
Colony Forming Units

The purpose of thisin vitrostudy was to assess the effect of hydrogen peroxide on the antibacterial substantivity of chlorhexidine (CHX). Seventy-five dentine tubes prepared from human maxillary central and lateral incisor teeth were used. After contamination withEnterococcus faecalisfor 14 days, the specimens were divided into five groups as follows: CHX, H2O2, CHX + H2O2, infected dentine tubes (positive control), and sterile dentine tubes (negative control). Dentine chips were collected with round burs into tryptic soy broth, and after culturing, the number of colony-forming units (CFU) was counted. The number of CFU was minimum in the first cultures in all experimental groups, and the results obtained were significantly different from each other at any time period (). At the first culture, the number of CFU in the CHX + H2O2group was lower than other two groups. At the other experimental periods, the CHX group showed the most effective antibacterial action (). Hydrogen peroxide group showed the worst result at all periods. In each group, the number of CFU increased significantly by time lapse (). In conclusion, H2O2had no additive effect on the residual antibacterial activity of CHX.

scholarly journals Application of XP-Endo finisher files in the reduction of microbial load in oval-shaped root canals - An ex vivo study

2019 ◽  
Author(s):  
Giovanna Dornelas Mantovani ◽  
Brenda Paula Figueiredo de Almeida Gomes ◽  
Lidiane Mendes Louzada ◽  
Rodrigo Arruda Vasconcelos ◽  
Maria Cristina Carvalho ◽  
...  
Keyword(s):  
Saline Solution ◽  
Ex Vivo ◽  
Microbial Reduction ◽  
Bacterial Reduction ◽  
Significance Level ◽  
Root Canals ◽  
Colony Forming Units ◽  
Post Hoc ◽  
Cleaning Efficacy ◽  
Ex Vivo Study

The present study evaluated the effectiveness of XP-Endo Finisher (XPF) associated with XP-Endo Shaper (XPS) or Reciproc Blue (RB) files in the reduction of Enterococcus faecalis in oval-shaped root canals. Eighty mandibular incisors with single oval-shaped root canals were contaminated with E. faecalis for 10 days at 37°C with centrifugation on alternate days. The teeth were randomly assigned to eight experimental groups (n=10) as follows: G1 – XP-Endo Shaper, G2 – XP-Endo Shaper + XP-Endo Finisher, G3 – Reciproc Blue, and G4 – Reciproc Blue + XP-Endo Finisher. Chemomechanical preparation was performed with saline solution (NaCl) or 2.5% sodium hypochlorite (NaOCl). Microbial reduction was assessed by colony forming units (CFU/mL) count before (S1) and after (S2) CMP by using sterile paper points. ANOVA, Tukey’s test and Bonferroni’s post-hoc tests were used at 5% significance level. Bacteria were present in all initial samples (P>.05). All instrumentation techniques reduced bacteria, irrespective of the irrigating solution (P<.05). With the use of NaCl, Reciproc Blue was more effective than XP-Endo Shaper (P=.035). The association of XP-Endo Finisher improved the cleaning efficacy of both systems (P=.239). With the use of NaOCl, XP-Endo Shaper and Reciproc Blue presented similar effectiveness (P=.779). XP-Endo Finisher enhanced the bacterial reduction of both systems tested (P<.05). The use of NaOCl improved the CMP, irrespective of the instrumentation technique used (P<.05).

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