The Healing Effect of Aloe Vera Gel on Acetic Acid-Induced Ulcerative Colitis in Rat

BACKGROUND The use of herbal and synthetic compounds can be effective in improving the areas and repair of tissues that have been affected during the processes like what happens in ulcerative colitis (UC) as a common inflammatory disorder. According to the beneficial effects of aloe vera, in this study, we aimed to assess the therapeutic effects of oral aloe vera gel on acetic acid-induced colitis in rats by histopathological and molecular analysis of Bax, and BCL-2 genes expression (using RT-PCR technique) in colon tissue samples. METHODS This experimental study comprised 32 adult male Sprague Dawley rats weighting 220 ± 20 g that were randomly divided into four groups as follows. The control group (healthy rats), colitis group in which UC was induced by transrectal administration of 3% acetic acid with no treatment, oral form of sulfasalazine group in which UC was induced by transrectal administration of 3% acetic acid, then was treated by oral administration of sulfasalazine 500 mg/kg body weight, and the fourth group which received oral form of aloe vera gel (200 mg / kg) for 21 days, respectively after induction of UC. Then, the therapeutic effects of treatment groups were compared with the control group and the colitis group with no treatment, by the assessment of histopathological and molecular changes in the colon tissues of rats on the 7th, 14th, and 21st days. Finally, the collected data were analyzed using statistical tests. RESULTS Histologically, aloe vera gel treatment could reduce and heal colon tissue damages in induced colitis. Also, this gel reduced apoptosis in rat’s colon with acetic acid-induced colitis, which showed in significantly decreased in Bax mRNA expression and significantly increased BCL-2 mRNA expression compared with the colitis group with no treatment. CONCLUSION Aloe vera gel has a significant effect on the treatment of UC in rat because of the beneficial effect that was found from aloe vera such as decreasing the severity of colitis as evidenced by histopathological findings, and with respect to apoptosis and gene expression that were related to wound healing process, and suppression of the elevation of Bax mRNA with the upregulation of Bcl-2, which can be considered effective in the treatment of UC.

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Protective and Therapeutic Effects of Aloe Vera Gel on Ulcerative Colitis Induced by Acetic Acid in Rats

Clinical Nutrition Research ◽

10.7762/cnr.2020.9.3.223 ◽

2020 ◽

Vol 9 (3) ◽

pp. 223

Author(s):

Gholamreza Bahrami ◽

Hossein Malekshahi ◽

Shahram Miraghaee ◽

Hamid Madani ◽

Atefeh Babaei ◽

...

Keyword(s):

Ulcerative Colitis ◽

Acetic Acid ◽

Aloe Vera ◽

Therapeutic Effects ◽

Aloe Vera Gel

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Electro-acupuncture on Lower He-sea Points: How Does it Affect Serum HMGB1 and Tissue α7 nAChR in Ulcerative Colitis Rats

Journal of Acupuncture and Herbs ◽

10.1515/tcm-2015-0006 ◽

2015 ◽

Vol 3 (1) ◽

pp. 46-54

Author(s):

Hong Zhang

Keyword(s):

Ulcerative Colitis ◽

Mrna Expression ◽

Tissue Injury ◽

Control Group ◽

Colon Tissue ◽

Blank Control Group ◽

Benzene Sulfonic Acid ◽

Treatment Groups ◽

Α7 Nachr ◽

Relative Specificity

Abstract Objective: To observe the expression levels of serum high mobility group box 1 (HMGB1) , nicotinic acetylcholine receptor α7 (α7 nAChR) and nuclear factor kappa-B mRNA(NF-κB mRNA) in colon tissue after electroacupuncture (EA) on Shangjuxu, Zusanli, Xiajuxu,Yanglingquan and Chengjin points, by comparison of the different effects we could thus explore the relative specificity of Shangjuxu on treating its corresponding viscera’ s disease. Methods: Seventy SD rats were randomly assigned to seven groups labeled by blank control (A), Model (B), Shang Ju Xu (C), Zu San Li (D), Xia Ju Xu (E), Yang Ling Quan (F), Cheng Jin (G), ten (half males and half females) in each group. Except for the blank control group, animals in other groups were modeled to ulcerative colitis (UC) by lavation of 2-4-6 trinitro-benzene-sulfonic acid (TNBS)/ethanol solution. After successful modeling animals in the five treatment groups were respectively treated with electroaccupuncture at corresponding acupoint for 10 consecutive days. Then histological changes in the colon were observed by light microscopy. ELISA method was used to check models’serum HMGB1 content, and α7 nAChR in the colon were detected by western blot , RT-PCR was used to detecte NF-κB mRNA. Results: ① Compared with the model group, the tissue injury changes in 5 treatment groups improved to a certain extent, and the colon tissue NF-κB mRNA expression of them decreasd signifcantly（P<0.01),as well, the α7 nAChR expression of Shang Ju Xu group, Zu San Li group and Xia Ju Xu group increased signifcantly (P<0.01 or P<0.05), and their serum HMGB1 content made obvious decrease(P<0.01 or P<0.05), the α7 nAChR expression in Yang Ling Quan group was also increased significantly（P<0.01). ② Compared with Shang Ju Xu group, the tissue injury changes of groups including Xia Ju Xu,Yang Ling Quan and Cheng Jin are much severe under light microscope, and the α7 nAChR expression of them decreased with significance（P<0.01),both serum HMGB1 content and colon tissue NF-κB mRNA expression increased significantly as well（P<0.01),the serum HMGB1 content of Zu San Li group also increased significantly（P<0.01). Conclusion: ① Electroacupuncture on all five different acupoints can improve the UC through regulating HMGB1, α7 nAChR and NF-κB mRNA, and effectively inhibiting the immune response, reducing inflammatory response of colon and improving pathological changes of colonic mucosa. ②The effect of treating UC inflammation in rats when electroacupuncture at Shang Ju Xu was better than Zu San Li, Xiajuxu, Yang Ling Quan and Cheng Jin, which demonstrated that Shangjuxu treating UC has some relative specificity.

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Effect of a Polyphenol-Rich Extract from Aloe vera Gel on Experimentally Induced Insulin Resistance in Mice

The American Journal of Chinese Medicine ◽

10.1142/s0192415x07005491 ◽

2007 ◽

Vol 35 (06) ◽

pp. 1037-1046 ◽

Cited By ~ 39

Author(s):

Yolanda Y. Pérez ◽

Enrique Jiménez-Ferrer ◽

Alejandro Zamilpa ◽

Marcelino Hernández-Valencia ◽

Francisco J. Alarcón-Aguilar ◽

...

Keyword(s):

Insulin Resistance ◽

Body Weight ◽

Aloe Vera ◽

Negative Control Group ◽

Negative Control ◽

Control Group ◽

Glucose Levels ◽

Insulin Tolerance ◽

Aloe Vera Gel ◽

Experimentally Induced

Insulin resistance, which precedes type 2 diabetes mellitus (T2DM), is a widespread pathology associated with the metabolic syndrome, myocardial ischemia, and hypertension. Finding an adequate treatment for this pathology is an important goal in medicine. The purpose of the present research was to investigate the effect of an extract from Aloe vera gel containing a high concentration of polyphenols on experimentally induced insulin resistance in mice. A polyphenol-rich Aloe vera extract (350 mg/kg) with known concentrations of aloin (181.7 mg/g) and aloe-emodin (3.6 mg/g) was administered orally for a period of 4 weeks to insulin resistant ICR mice. Pioglitazone (50 mg/kg) and bi-distilled water were used as positive and negative controls respectively. Body weight, food intake, and plasma concentrations of insulin and glucose were measured and insulin tolerance tests were performed. The insulin resistance value was calculated using the homeostasis model assessment for insulin resistance (HOMA-IR) formula. Results showed that the polyphenol-rich extract from Aloe vera was able to decrease significantly both body weight ( p < 0.008) and blood glucose levels ( p < 0.005) and to protect animals against unfavorable results on HOMA-IR, which was observed in the negative control group. The highest glucose levels during the insulin tolerance curve test were in the negative control group when compared to the Aloe vera extract and pioglitazone treated mice ( p < 0.05). In conclusion, Aloe vera gel could be effective for the control of insulin resistance.

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Efficacy of a New Non-drug Acne Therapy: Aloe Vera Gel Combined With Ultrasound and Soft Mask for the Treatment of Mild to Severe Facial Acne

Frontiers in Medicine ◽

10.3389/fmed.2021.662640 ◽

2021 ◽

Vol 8 ◽

Author(s):

Hongyu Zhong ◽

Xiang Li ◽

Wanqi Zhang ◽

Xiaoxiao Shen ◽

Yuangang Lu ◽

...

Keyword(s):

Treatment Group ◽

Side Effects ◽

Combined Therapy ◽

Aloe Vera ◽

Physical Method ◽

Control Group ◽

Severe Acne ◽

Aloe Vera Gel ◽

Before And After ◽

Acne Therapy

Background: Acne is a chronic disorder that affects almost 80% of adolescents and young adults, causing psychological and emotional distress. However, the current treatments for acne are either ineffective or have many side effects. This study was designed to confirm and objectively quantify the effect of a new non-drug combined therapy on acne.Methods: This study innovatively utilized ultrasound, which enhanced the absorption of aloe vera gel, and soft mask to make a purely physical method without any drugs. In both the treatment group and control group, the number of papules/pustules and the area of hyperpigmented lesions were counted, and a smart mirror intelligent face system was used before and after the combined therapy. Alterations in the skin functional index were recorded and analyzed statistically.Results: In the treatment group, the combined therapy significantly reduced the number of papules and the area of hyperpigmented lesions and improved skin roughness and local blood circulation. In the control group, there was no obvious improvement over 2 months.Conclusion: This study suggests that the new non-drug combined therapy significantly improved acne, which provided experimental evidence and treatment guidance for patients with mild to severe acne, especially patients with moderate acne. This new therapy may possibly be an appropriate method for patients who seek topical treatments with mild side effects and low antibiotic resistance rates.

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The Effect of Aloe vera (Linn) On Cutaneous Wound Healing and Wound Contraction Rate in Adult Rabbits

Nova Journal of Medical and Biological Sciences ◽

10.20286/jmbs-050307 ◽

2016 ◽

Vol 5 (3) ◽

Cited By ~ 1

Author(s):

Martha Orendu Attah ◽

Tonye Watson Jacks ◽

Attah Jacob ◽

Otong Eduitem ◽

Barnabas John

Keyword(s):

Wound Healing ◽

Aloe Vera ◽

Wound Contraction ◽

Control Group ◽

Cutaneous Wound Healing ◽

Contraction Rate ◽

Wound Area ◽

Cutaneous Wound ◽

Cutaneous Wounds ◽

Aloe Vera Gel

Background: In the present research study, the rate of cutaneous wound healing and contraction rate in healthy rabbits using Aloe vera pulp was studied.Methods: Ten healthy rabbits were used for the study. They were divided into two groups consisting of five rabbits each. Cutaneous wounds were made on the lumbar region of each rabbit using a template which ensured that the wounds were of the same size in all the rabbits. 5ml of Aloe vera gel was applied to the wounds of the animals in the test group, while nothing was applied to the wound area of the animals in the control group. The wound area in each group was measured for a period of 21 days, using a venire caliper and tracing paper which was used to trace the wound area. Tissue samples were removed from the wound area in both experimental and control groups and subjected to routine histological analysis, also, morphometric analysis was performed.Results: The rate of wound contraction and mean centripetal contraction was calculated in both groups and graphically represented using Microsoft Excel. The results showed that animals who were treated with Aloe vera gel had a greater wound contraction rate, as well as rapid wound closure. The micrographs showed a thicker epithelial layer, with thinner collagen fibers in the dermis of experimental animals compared to the control group. There was also an abundant capillary bed at the dermal-epidermal junction in the experimental group, compared to the control group.Conclusion: Aloe vera may increase the rate of wound healing by accelerating epithelial migration, and may also play a role in neo-vascularization of the newly healed area.Keywords: Aloe vera, Cutaneous Wounds, Rabbits, Wound Contraction, Wound Healing

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Ginger Alleviates DSS-Induced Ulcerative Colitis Severity by Improving the Diversity and Function of Gut Microbiota

Frontiers in Pharmacology ◽

10.3389/fphar.2021.632569 ◽

2021 ◽

Vol 12 ◽

Author(s):

Shanshan Guo ◽

Wenye Geng ◽

Shan Chen ◽

Li Wang ◽

Xuli Rong ◽

...

Keyword(s):

Ulcerative Colitis ◽

Gut Microbiota ◽

Pathogenic Bacteria ◽

Intestinal Flora ◽

Experimental Models ◽

Intestinal Microbiome ◽

Control Group ◽

Therapeutic Effects ◽

Sequencing Analysis ◽

And Function

The effects of ginger on gastrointestinal disorders such as ulcerative colitis have been widely investigated using experimental models; however, the mechanisms underlying its therapeutic actions are still unknown. In this study, we investigated the correlation between the therapeutic effects of ginger and the regulation of the gut microbiota. We used dextran sulfate sodium (DSS) to induce colitis and found that ginger alleviated colitis-associated pathological changes and decreased the mRNA expression levels of interleukin-6 and inducible nitric oxide synthase in mice. 16s rRNA sequencing analysis of the feces samples showed that mice with colitis had an intestinal flora imbalance with lower species diversity and richness. At the phylum level, a higher abundance of pathogenic bacteria, Proteobacteria and firmicutes, were observed; at the genus level, most samples in the model group showed an increase in Lachnospiraceae_NK4A136_group. The overall analysis illustrated an increase in the relative abundance of Lactobacillus_murinus, Lachnospiraceae_bacterium_615, and Ruminiclostridium_sp._KB18. These increased pathogenic bacteria in model mice were decreased when treated with ginger. DSS-treated mice showed a lower abundance of Muribaculaceae, and ginger corrected this disorder. The bacterial community structure of the ginger group analyzed with Alpha and Beta indices was similar to that of the control group. The results also illustrated that altered intestinal microbiomes affected physiological functions and adjusted key metabolic pathways in mice. In conclusion, this research presented that ginger reduced DSS-induced colitis severity and positively regulated the intestinal microbiome. Based on the series of data in this study, we hypothesize that ginger can improve diseases by restoring the diversity and functions of the gut microbiota.

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Comparative Evaluation of Aloe vera and Diclofenac on Body Weight, Blood Pressure and Renal Function of Hypertensive Rats

Proceedings of Shaikh Zayed Medical Complex Lahore - October to December ◽

10.47489/p000s351z7821-6mc ◽

2021 ◽

Vol 35 (1) ◽

pp. 39-44

Author(s):

Nadeem Yaqoob

Keyword(s):

Blood Pressure ◽

Renal Function ◽

Body Weight ◽

Systolic Blood Pressure ◽

Aloe Vera ◽

Treated Group ◽

Control Group ◽

Hypertensive Rats ◽

Aloe Vera Gel ◽

Group B

Introduction: NSAIDs are known to cause salt and water retention leading to hypertension and renal impairment. Aloe vera gel has been used in medicinal preparations for decades. Limited data is available regarding effect of Aloe vera on renal function. There is a need to search this aspect of Aloe vera, to use it judiciously. Aims & Objectives: To estimate and compare the effects of Aloe vera and diclofenac on systolic blood pressure and renal functions of hypertensive rats. Place and duration of study: This study was conducted at Post Graduate Medical Institute Lahore, Sargodha Medical College, Sargodha and Department of Pharmacy, University of Sargodha for the period of three months. Material & Methods: Male Sprague Dawley rats (n=24) were divided into four groups; Group A (Normal control), Group B (Hypertensive control), Group C (Aloe vera treated) and Group D (Diclofenac treated). Hypertension was induced in groups B, C and D by 20% sucrose diet in 8 weeks. After induction of hypertension, distilled water, dried Aloe vera gel 400 mg/kg and diclofenac 12 mg/kg were given orally to group B, C and D respectively for 2 weeks as a single morning dose. Body weight and systolic blood pressure were measured weekly, while serum creatinine, creatinine clearance and urinary proteins were estimated and compared at 0, 8 and 10 weeks. Data was analyzed using SPSS 23 and p value of ?0.05 was considered significant. Results: Diclofenac decreased body weight of rats non-significantly and increased systolic blood pressure significantly (p< 0.03) whereas Aloe vera increased body weight significantly (p<0.012) and had no significant effect on systolic blood pressure. Diclofenac treated group showed deterioration of renal function as compared to Aloe vera treated group numerically. Conclusion: Aloe vera may be safer anti-inflammatory agent than diclofenac for treatment of chronic inflammatory conditions if the patient also has hypertension or renal disease.

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Effects of Dried Aloe Vera Gel and Diclofenac on Sodium and Potassium Homeostasis: An Experimental Study on Hypertensive Rats

Journal of Islamabad Medical & Dental College ◽

10.35787/jimdc.v10i3.658 ◽

2021 ◽

Vol 10 (3) ◽

pp. 133-139

Author(s):

Nadeem Yaqoob ◽

Abdul Qudoos Arain ◽

Mufakhara Fatimah ◽

Samina Kausar ◽

Sadia Chiragh

Keyword(s):

Experimental Study ◽

Serum Potassium ◽

Aloe Vera ◽

Control Group ◽

Hypertensive Rats ◽

Aloe Vera Gel ◽

Group A ◽

Group B ◽

Group D ◽

Sodium And Potassium

Background: Anti-inflammatory role of Aloe vera gel is well established. Diclofenac is extensively used for acute and chronic inflammation. The present study was conducted to compare dried Aloe vera gel and diclofenac effects on sodium and potassium balance in hypertensive rats. Material and Methods: This experimental study was conducted at Sargodha Medical College from May to November 2016. Twenty-four healthy male Sprague Dawley rats 7-8 weeks of age were included in study. Any unhealthy-looking rat was excluded from the study. Rats were equally and randomly divided into four groups Normal control (group A), Model control (group B), Aloe vera (group C) & Diclofenac (group D). Hypertension was induced by a 20 % sucrose diet in all groups except group A in 8 weeks’ time. Group B, C & D received distilled water and Aloe vera dried gel 400 mg/kg & diclofenac powder 12 mg/kg body weight respectively orally between 8 to 10 weeks. Serum and urine analysis was performed for hematocrit, sodium, and potassium concentrations at zero, eight and ten weeks. Twenty-four-hour urinary sodium excretion was calculated. Data was analyzed using Graph Pad Prism version 6. Result: After 2-week administration of aloe vera and diclofenac powder, serum potassium significantly decreased in Group C (p <0.001) while increased in Group B and D (p <0.001) as compared to group A. Urinary sodium concentration and excretion increased significantly in Group C (p <0.01) as compared to Group A whereas result of Group D was insignificant. No significant change in serum sodium and hematocrit of any group was observed. Conclusion: Aloe vera causes less sodium retention than diclofenac but decreases serum potassium contrary to the effect of diclofenac in hypertensive rats.

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Potential of Mimosa pudica leaf in the treatment of ulcerative colitis in rat

Bangladesh Journal of Pharmacology ◽

10.3329/bjp.v13i3.35648 ◽

2018 ◽

Vol 13 (3) ◽

pp. 241

Author(s):

Bharati Zaware ◽

Ritu Gilhotra ◽

Sanjay Ravindra Chaudhari

Keyword(s):

Ulcerative Colitis ◽

Acetic Acid ◽

Acetic Acid Solution ◽

Video Clip ◽

Ethanolic Extract ◽

Mimosa Pudica ◽

Ulcer Index ◽

Colon Tissue ◽

Standard Drug ◽

Full Screen

The aim of present investigation was to validate its folk use in the treatment of ulcerative colitis in the rat model. Sprague Dawley rats were divided into nine groups with six animals in each group. The rats received seven days of pretreatment with chloroform, ethyl acetate or ethanolic extract of Mimosa pudica. For induction of ulcerative colitis, rats were administered with 2 mL of 4% acetic acid solution intrarectally. Ulcer index, macroscopical study of the colon, myeloperoxidase and malondialdehyde levels in colon tissue and blood, and histopathology of the colon tissue were studied. Intrarectal instillation of acetic acid caused increased ulcer index, colonic myeloperoxidase and malondialdehyde. Pretreatment with M. pudica ethanolic extract (400 mg/kg) significantly lowered the ulcer index, colonic myeloperoxidase and malondialdehyde as compared with the standard drug prednisolone. The present investigation demonstrates that the ethanol extract of M. pudica leaf is effective in the treatment of ulcerative colitis.Video Clip of Methodology:Embedding or block making: 24 sec <a href="https://www.youtube.com/v/Y1I4tKYMLsE">Full Screen</a> <a href="https://www.youtube.com/watch?v=Y1I4tKYMLsE">Alternate</a>Tissue processing: 19 sec <a href="https://www.youtube.com/v/7nYpxL2qPhg">Full Screen</a> <a href="https://www.youtube.com/watch?v=7nYpxL2qPhg">Alternate</a>Section cutting: 33 sec <a href="https://www.youtube.com/v/_amFL9kNLBw">Full Screen</a> <a href="https://www.youtube.com/watch?v=_amFL9kNLBw">Alternate</a>

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Efficacy of Aloe Vera Gel in Dry Socket After Removal of Mandibuler Third Molar

10.53350/pjmhs2115123530 ◽

2021 ◽

Vol 15 (12) ◽

pp. 3530-3532

Author(s):

Raj Kumar ◽

Fida Hussain ◽

Muhammad Ilyas ◽

Lavina . ◽

Ashfaque Ahmed ◽

...

Keyword(s):

Maxillofacial Surgery ◽

Aloe Vera ◽

Third Molar ◽

Control Group ◽

Surgical Removal ◽

Dry Socket ◽

Healing Index ◽

Aloe Vera Gel ◽

Group A ◽

Group B

Objective: To evaluate the effectiveness of Aloe Vera gel in management of dry socket after surgical removal of 3rd molar. Study Design & Setting: From December 2020 to November 2021, a descriptive research was conducted in the Section of Oral & Maxillofacial Surgery, Liaquat University of Medical & Health Sciences, Jamshoro, Hyderabad, Sindh. Methodology: Total 70 patients were included and divided equally in two groups i.e. Group-A (Alveora dressing) and Group-B (Controls). Dry socket, pre-operative assessment was carried out on the basis of pain Visual analog scale (VAS) and healing index. Socket was irrigated with sterile saline 0.9% in experimental group. In control group, patient were given tablet Panadol 1gm SOS. Patients were evaluated post operatively at 1st, 2nd, and 3rd day on the basis of pain (VAS) and healing index. Results: In group-A mean postoperative VAS at day-1, day-2 and day-3 was 6.69±2.59, 3.17±2.39 and 1.14±1.03 respectively. In group-B it was 7.77±2.03, 6.97±2.14 and 4.57±2.57. In group-A, mean postoperative healing scale at day-1, day-2 and day-3 was 2.83±0.56, 3.49±0.61 and 4.37±0.73 respectively and in group-B it was 2.31±0.58, 2.63±0.69 and 3.26±0.65 respectively. Significant association of pain intensity at day-2 and day-3 and healing index (at day-1, day-2 and day-3) was observed. Conclusion: Aloe Vera gel significantly reduced postoperative pain and improve healing index. Keywords: Effectiveness, Aloe Vera Gel, Dry Socket, Surgical Removal, Third Molar

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