scholarly journals Uji Daya Hambat Sediaan Sabun Transparan Ekstrak Jarak Pagar (Jatropha curcas) Terhadap Pertumbuhan Bakteri Uji Propionibacterium acnes

2017 ◽  
Vol 3 (02) ◽  
pp. 103-109
Author(s):  
Risky Juliansyah ◽  
Rismawati Paotonan
Keyword(s):  
Staphylococcus Aureus ◽  
Jatropha Curcas ◽  
Staphylococcus Epidermidis ◽  
Propionibacterium Acnes ◽  
Propionibacterium Acne

Jerawat  merupakan  penyakit  peradangan  yang  terjadi  akibat  penyumbatan  pada pilosebasea  yang  ditandai  dengan  adanya  komedo,  papul,  pastul,  dan  bopeng (scar) pada daerah  wajah,  leher,  lengan  atas,  dada,  dan  punggung.  Peradangan  dipicu  oleh Propionibacterium  acne,  Staphylococcus  epidermidis, dan Staphylococcus  aureus. Kandungan fitokimia dalam daun Jarak pagar yaitu tanin, steroid dan triterpenoid, flavanoid, saponin, antraquinon, dan alkaloid. Dalam formulasi sabun transparan penambahan bahan aktif tanaman dapat membantu peningkatan aktivitas antibakteri.                Dari hasil penelitian uji daya hambat sabun transparan ekstrak  jarak pagar  ( Jatropha curcas) terhadap pertumbuhan Bakteri Propionibacterium acnes menghasilkan zona hambatan pertumbuhan pada bakteri dengan konsentrasi hambatan minimum  yaitu 5 %. Untuk kontrol positif sendiri digunakan ekstrak jarak pagar  memberikan diameter  hambat sebesar 11,02 mm serta pada kontrol negatif digunakan sabun transparan tanpa penambahan ekstrak jarak pagar  memberikan diameter hambatan sebesar 5,32 mm. Sehingga konsentrasi minimum pada penghambatan pertumbuhan bakteri uji pada sediaan sabun transparan pada  konsentrasi  5%  dengan diameter hambatan 9,07 mm.  

scholarly journals Aktivitas Antibakteri Ekstrak Daun Jarak Pagar (Jatropha Curcas L) Terhadap Staphylococcus Epidermidis Dan Staphylococcus Aureus Secara In Vitro

2018 ◽  
Vol 7 (1) ◽  
Author(s):  
Susilo Yulianto ◽  
Sunarmi Sunarmi
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Jatropha Curcas ◽  
Staphylococcus Epidermidis ◽  
Leaf Extract ◽  
Inhibitory Zone ◽  
Zone Diameter ◽  
Jatropha Curcas L ◽  
Inhibitory Power ◽  
And Staphylococcus Aureus

Abstract: Jatropha Leaves, Staphylococcus Epidermidis, Staphylococcus Aureus. Jatropha curcas L leaves contain tannins, saponins and flavonoids. According to some studies, tannins, saponins and flavonoids have antibacterial power. Based on these indications, it is important to do research on antibacterial potency of jatropha leaf extract (Jatropha curcas L) against Staphylococcus epidermidis and Staphylococcus aureus bacteria. The aim of this research is to know Antibacterial Activity of Jatropha curcas L Extract on Staphylococcus epidermidis and Staphylococcus aureus". This research. An experimental study by testing the antibacterial activity of leaf extract and jatropha result of maceration, infundation and reflux as measured by Minimal Inhibitory Concentration (KHM) on growth of Staphylococcus epidermidis and Staphylococcus aureus bacteria. From the results of the research, it was found that the extract of the reflux was brownish green and the characteristic of jatropha leaves. The amount of extract produced was 17.03 g, so the yield was 17.03%. Jatropha leaf extract of maceration has no inhibitory power to S. epidermidis in 4 series concentrations starting from 3.125 ppm to 25.000 ppm. Inhibitory zones begin to appear at concentrations of 50,000 ppm and increase at concentrations of 100,000 ppm. The drag zone diameter in the Jatropha curcas extract of the reflux showed the inhibitory power in all series of concentrations. The smallest inhibitory zone was shown at a concentration of 3,125 ppm (9.33 mm) and increased with increasing concentrations. The diameter of the inhibit zone was greatest at 100,000 ppm concentrations of 22.67 mm. Description of inhibitory zone diameter of methanol leaf extract of jatropha result of maceration and reflux to S. epidermidis. The conclusion of this research is extract of reflux result giving bigger resistance than maceration extract to S. epidermidis and S. aureus. Methanol extract of jatropha from the maceration and reflux have strong antibacterial activity against S. epidermidis and S. aureus but still under Klindamycin.

scholarly journals PENGARUH SUHU DAN LAMA PENYIMPANAN SEDIAAN KRIM ANTI JERAWAT MENGANDUNG ANTIBIOTIK YANG DIRACIK DI APOTEK TERHADAP AKTIVITAS ANTIBAKTERI STAPHYLOCOCCUS AUREUS

2019 ◽  
Vol 13 (2) ◽  
pp. 80-84
Author(s):  
Nizar Nizar ◽  
Sarmadi Sarmadi ◽  
RF Pitaloka
Keyword(s):  
Staphylococcus Aureus ◽  
Staphylococcus Epidermidis ◽  
Propionibacterium Acnes

Latar Belakang : Jerawat merupakan penyakit kulit yang umum terjadi, peradangan dapat dipicu oleh bakteri seperti Propionibacterium acnes, Staphylococcus epidermidis dan Staphylococcus aureus. Sediaan antibiotik topikal seperti neomycin, tetrasiklin, klindamisin, dan kloramfenikol cukup berguna untuk kebanyakan pasien dengan kondisi jerawat ringan hingga parah. Antibiotik topikal dapat berupa salep dan krim yang dapat mengalami perubahan karena dipengaruhi oleh suhu sehingga penyimpanannya harus diperhatikan.Metode : Jenis penelitian ini adalah penelitian eksperimental karena ada perlakuan terhadap sediaan krim antijerawat racikan yang dipengaruhi suhu dan lama penyimpanan terhadap aktivitas antibakteri dengan cara mengukur diameter zona hambat aktivitas antibakteri. Hasil:Berdasarkan hasil pengukuran diameter zona hambat pada sediaan krim antijerawat racikan yang mengandung antibiotik pada penyimpanan hari ke-28 sediaan mengalami penurunan kecuali pada krim A yang mengandung antibiotik klindamisin mengalami kenaikan.Kesimpulan : Adanya pengaruh suhu dan lama penyimpanan sediaan krim antijerawat racikan yang mengandung antibiotik terhadap daya hambat bakteri Staphylococcus aureusdengan adanya penurunan daya hambat sediaan diakhir penyimpanan.

scholarly journals Antibacterial Activity of Extract and Fraction From Shitake Mushroom (Lentinula edodes) Against Acne Bacteria

2018 ◽  
Vol 6 (1) ◽  
pp. 36
Author(s):  
Ika Kurnia Sukmawati ◽  
Ari Yuniarto ◽  
Widhya Alighita ◽  
Ade Zam-zam J
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Staphylococcus Epidermidis ◽  
Propionibacterium Acnes ◽  
Ethyl Acetate Fraction ◽  
Hexane Fraction ◽  
Lentinus Edodes ◽  
Shiitake Mushrooms ◽  
And Staphylococcus Aureus

Acne is an inflammatory disease that occurs in the skin triggered by the bacteria acne treatment can be done by using natural materials that shiitake mushrooms (Lentinus edodes). This aims of this study were to determining the antibacterial activity of extracts and fractions shiitake mushrooms with broth microdilution method, determining the value of equality shiitake mushrooms with antibacterial comparison and determining the morphological changes of bacteria after exposure to the test sample with a Scanning Electron Microscope (SEM). Tests conducted antibacterial activity against Propionibacterium acnes, Staphylococcus epidermidis and Staphylococcus aureus at concentrations used is 1 ppm, 2 ppm, 4 ppm, 8 ppm, 16 ppm, 32 ppm, 64 ppm, 128 ppm, 256 ppm, 512 ppm. Best MIC value obtained in fraction of ethyl acetate and n-hexane fraction of the bacterium Propionibacterium acnes, Staphylococcus epidermidis and Staphylococcus aureus at a concentration of 256 ppm. KBM value of the n-hexane fraction against Staphylococus aureus bacteria at concentrations of 512 ppm and ethyl acetate fraction against bacteria Staphylococus aureus and epidermidis at a concentration of 512 ppm. Value equality is obtained 1 mg of ethyl acetate fraction of shiitake mushrooms equivalent to 5.346 x 10-2 mg of tetracycline. SEM test results showed the presence of antibacterial activity which is indicated by a change in cell morphology, their lumps and their cell wall frown on Propionibacterium acnes were exposed to ethyl acetate fraction.Key words: Acne, Lentinus edodes, antibacterials, microdilution, SEM

Susceptibility of Propionibacterium acnes, Staphylococcus aureus and Staphylococcus epidermidis to 10 Kampo Formulations

1997 ◽  
Vol 25 (6) ◽  
pp. 318-324 ◽  
Author(s):  
S Higaki ◽  
S Mommatsu ◽  
M Morohashi ◽  
T Yamagishi ◽  
Y Hasegawa
Keyword(s):  
Staphylococcus Aureus ◽  
Staphylococcus Epidermidis ◽  
Propionibacterium Acnes ◽  
Herbal Medicines ◽  
Skin Infections ◽  
Common Cause ◽  
Chinese Herbal ◽  
Crude Drugs ◽  
And Staphylococcus Aureus

We examined the in vitro sensitivities of three bacteria: Propionibacterium acnes, and Staphylococcus epidermidis, commonly detected in acne lesions, and Staphylococcus aureus, a common cause of skin infections, to 10 Kampo formulations (Chinese herbal medicines; combinations of powdered extracts of crude drugs). Both Staphylococcus species showed similar sensitivities to all 10 formulations, with minimum inhibitory concentrations (MICs) ranging from 25 to 400 mg/ml. P. acnes, however, was particularly sensitive to one formulation, keigai-rengyo-to (MIC, 0.78 – 25 mg/ml), prompting speculation that it might contain components with strong antibacterial activity to P. acnes. P. acnes showed similar sensitivities to all the other formulations (MIC 6.25 – 200 mg/ml). The ranges of MICs and the MIC50s (concentrations that inhibit 50% of isolates) were very similar to those previously recorded in 1990 for the two Staphylococcus species.

scholarly journals FORMULASI DAN PENGUJIAN STABILITAS FISIK GEL ANTIJERAWAT LIOFILISAT LIMBAH KOKON ASAL KABUPATEN SOPPENG

2019 ◽  
Vol 2 (2) ◽  
pp. 70
Author(s):  
An Maria Redi Dambur ◽  
Rifka Malluka ◽  
Nurafiat Anton ◽  
Sukriani Kursia
Keyword(s):  
Staphylococcus Aureus ◽  
Bombyx Mori ◽  
Staphylococcus Epidermidis ◽  
Dosage Form ◽  
Physical Stability ◽  
Propionibacterium Acne ◽  
Carbopol 940 ◽  
Bombyx Mori L ◽  
Good Preparation ◽  
Silkworm Bombyx Mori

ABSTRACT Lyophilisate of cocoon silkworm (Bombyx mori L.) possess antibacterial activity against Propionibacterium acne, Staphylococcus epidermidis, and S. aureus which belong to the group of bacteria that cause acne. Therefore, this research was carried out to make a formula for anti-acne gel based on Carbopol 940®. The gel dosage form was chosen because it is easier to clean from the surface of the skin after use and contains no oil which can increase the severity of acne. The formulation was tested on the physical stability of the preparations in the form of organoleptic, homogeneity, pH, dispersibility, adhesion and viscosity tests. The results of the study showed that the anti-acne gel formulat had a good preparation quality and anti-acne activity. Keywords : Cocoon, gel, anti-acne, Propionibacterium acne, Staphylococcus epidermidis, Staphylococcus aureus   ABSTRAK Liofilisat kokon ulat sutera (Bombyx mori L.) memiliki aktivitas antibakteri terhadap Propionibacterium acne, Staphylococcus epidermidis, dan S. aureus yang merupakan bakteri penyebab jerawat. Oleh karena itu, penelitian ini dilakukan untuk membuat formula sediaan gel antijerawat dengan basis gel Carbopol 940®. Bentuk sediaan gel dipilih karena lebih mudah dibersihkan dari permukaan kulit setelah pemakaian dan tidak mengandung minyak yang dapat meningkatkan keparahan jerawat. Hasil formulasi dilakukan uji stabilitas fisik sediaan berupa uji organoleptik, homogenitas, pH, daya sebar, daya lekat dan viskositas. Hasil penelitian menunjukkan bahwa formulagel antijerawat liofilisat kokon memiliki kualitas sediaan yang baik. Kata kunci: Kokon, gel, antijerawat, Propionibacterium acne, Staphylococcus epidermidis, Staphylococcus aureus

scholarly journals UJI AKTIVITAS KOMBINASI EKSTRAK KERING LIDAH BUAYA (Aloe vera. (L) brum. f.) DAN EKSTRAK KENTAL DAUN SIRIH MERAH (Piper crocatum ruiz & pav) UNTUK ANTIBAKTERI PENYEBAB JERAWAT

2018 ◽  
Vol 15 (01) ◽  
pp. 12
Author(s):  
Suhaimi Suhaimi ◽  
Teti Indrawati ◽  
Shirly Kumala
Keyword(s):  
Staphylococcus Aureus ◽  
Leaf Extract ◽  
Propionibacterium Acnes ◽  
Aloe Vera ◽  
Inhibitory Effect ◽  
Dry Extract ◽  
Propionibacterium Acne ◽  
Betel Leaf ◽  
And Staphylococcus Aureus

INTISARI   Ekstrak kering lidah buaya dan ekstrak kental daun sirih merah mengandung senyawa alkaloid, flavonoid, tanin, saponin dan fenol yang mempunyai aktivitas sebagai antibakteri penyebab jerawat. Penelitian ini bertujuanUntuk menentukan konsentrasi ekstrak kering lidah buaya  (Aloe vera (L) brum f), untuk menentukan ekstrak kental daun sirih merah (Piper crotacum ruiz dan pav)  mempunyai daya hambat ter terhadap bakteri Propionibacterium acnes dan Staphylococcus aureus penyebab jerawat dan untuk menentukan konsentrasi kombinasi ekstrak kering lidah buaya  (Aloe vera (L) brum f)  dan ekstrak kental daun sirih merah  (Piper crotacum ruiz dan pav)mempunyai daya hambat terterhadap bakteri  Propionibacterium acnes  dan  Staphylococcus aureus  penyebab jerawat.Tahap-tahap penelitian yaitu determinasi tanaman lidah buaya dan daun sirih merah, pembuatan ekstrak, pemeriksaan ekstrak meliputi organoleptik, pH, uji skrining fitokimia serta pengujian aktivitas masing-masing ekstrak kering lidah buaya dengan konsentrasi 10%, 15%, 20%, 25%, 30% dan 35%, ekstrak kental daun sirih merah dengan konsentrasi 25%, 12,5%, 7,16%, 3,13%, 1,78% dan 0,78% serta kombinasi ekstrak kering lidah buaya dan ekstrak kental daun sirih merah dengan konsentrasi 50%, 25%, 12,5%, 7,16%, 3,13%,  dan 1,56 terhadap bakteri Propionibacterium acnes dan Staphylococcus aureus. Kombinasi ekstrak kering  lidah buaya  dan ekstrak kental daun sirih merah   memiliki aktivitas antibakteri terhadap bakteri  Staphylococcus aureus  pada konsentrasi 1,56 % dan Propionibacterium acne   pada konsentrasi  3,13  Kata kunci : Lidah buaya, daun sirih merah, Propionibacterium acnes, Staphylococcus aureus.  ABSTRACT  Dried aloe vera extract and thick red betel leaf extract contains alkaloid compounds, flavonoids, tannins, saponins and phenols that have activity as an antibacterial cause of acne. The aim of this study was to determine the concentration of Aloe vera (L) brum f) extract, to determine the thick red betel leaf extract (Piper crotacum ruiz and pav) to have the inhibitory effect against the bacteria Propionibacterium acnes and Staphylococcus aureus causes acne and  to determine the concentration of Aloe vera (L) brum f) and red thick betel leaf extract (Piper crotacum ruiz and pav) have inhibitory effect on bacteria Propionibacterium acnes and Staphylococcus aureus cause acne. The research stages are the determination of aloe vera and red betel leaf, extract preparation, extract examination including organoleptic, pH, phytochemical screening test and activity test of dry aloe vera extract with concentration 10%, 15%, 20%, 25% , 30% and 35%, thick red betel leaf extract with concentration of 25%, 12.5%, 7.16%, 3.13%, 1.78% and 0.78%, and combination of aloe vera extract and viscous extract red betel leaves with concentrations of 50%, 25%, 12.5%, 7.16%, 3.13%, and 1.56 against the bacteria Propionibacterium acnes and Staphylococcus aureus. The combination of dry extract of aloe vera and thick red betel leaf extract have antibacterial activity against Staphylococcus aureus bacteria at concentration 1.56% and Propionibacterium acne at concentration 3,13 %. Keywords: aloe vera, red betel leaf extract, Propionibacterium acnes, Staphylococcus aureus

scholarly journals FORMULASI DAN UJI EFEKTIVITAS GEL EKSTRAK BUAH PARE (Momordica charantia L) Terhadap BAKTERI Staphylococcusepidermidis dan Propionibacterium acnes PENYEBAB JERAWAT

2019 ◽  
Vol 2 (1) ◽  
Author(s):  
Nur Ain Thomas ◽  
Widy Susanti Abdulkadir ◽  
Mega Agustiwi Mohi
Keyword(s):  
Staphylococcus Epidermidis ◽  
Propionibacterium Acnes ◽  
Scientific Data ◽  
Momordica Charantia ◽  
Bitter Melon ◽  
Fruit Extract ◽  
Propionibacterium Acne ◽  
Extract Concentration ◽  
Irritation Test ◽  
Bitter Fruit

FORMULASI DAN UJI EFEKTIVITAS GEL EKSTRAK BUAH PARE (Momordica charantia L) Terhadap BAKTERI Staphylococcusepidermidis dan Propionibacterium acnes PENYEBAB JERAWAT Nur Ain Thomas1), Widysusanti Abdulkadir1), Mega Agustiwi Mohi1) 1)Jurusan Farmasi, Fakultas Olahraga dan KesehatanUniversitas Negeri Gorontalo ABSTRACT Acne is a skin disease in the form of inflammation in the layer of polysebaseus triggered by the bacteria Staphyloccoccus epidermidis and Propionibacterium acnes. One plant that is empirically and based on scientific data has anti-acne properties is bitter melon (Momordica charantia L). In pare fruit extract contains flavonoids which are thought to be able to act as active compounds in the form of anti-acne. This study aims to formulate bitter melon extract as an anti-acne gel and determine the inhibition of bitter melon extract gel against acne-causing bacteria, namely Staphylococcus epidermidis and Propionibacterium acne. Pare fruit extract gel with 3 variations of extract concentration, namely 5%, 7.5% and 10%. Evaluation of gel preparations included organoleptic test, homogeneity, spreadability, pH, viscosity, and irritation test. Data analysis using Oneway ANOVA. Based on the results of the research conducted, it can be concluded that bitter fruit extract (Momordica charantia L) can be formulated as an anti-acne gel, and the preparation of bitter fruit extract gel (Momordica charantia L) at a concentration of 10% has a inhibition of 10 mm against Staphylococcus epidermidis and 7, 1 mm against the bacterium Propionibacterium acne which is included in the medium category. Keywords: Acne, Extract, Gel , Stahpylococcus epidermidis, Propionibacterium acnes  ABSTRAK Jerawat merupakan penyakit kulit berupa peradangan pada lapisan polisebaseus yang dipicu oleh bakteri Staphyloccoccus epidermidis dan Propionibacterium acnes. Salah satu tanaman yang secara empiris dan berdasarkan data ilmiah memiliki khasiat antijerawat adalah buah pare (Momordica charantia L). Dalam ekstrak buah pare terkandung flavonoid yang diduga dapat berperan  sebagai senyawa aktif sedian antijerawat. Penelitian ini bertujuan untuk  memformulasikan ekstrak buah pare sebagai gel antijerawat dan menentukan daya hambat gel ekstrak buah pare terhadap bakteri penyebab jerawat yaitu Staphylococcus epidermidis dan Propionibacterium acne. Gel ekstrak buah pare dengan 3 variasi konsentrasi ekstrak yaitu 5%, 7,5% dan 10%. Evaluasi sediaan gel meliputi uji organoleptik, homogenitas, daya sebar, pH, viskositas, dan uji iritasi.Analisis data menggunakan ANOVA Oneway. Berdasarkan hasil penelitian yang dilakukan maka dapat disimpulkan bahwa ekstrak buah pare (Momordica charantia L) dapat diformulasikan sebagai gel antijerawat, dan sediaan gel ekstrak buah pare (Momordica charantia L) pada konsentrasi 10% mempunyai daya hambat 10 mm terhadap bakteri Staphylococcus epidermidis dan 7,1 mm terhadap bakteri Propionibacterium acne yang termasuk dalam kategori sedang.Kata Kunci :Jerawat, Ekstrak Buah Pare, Gel, Stahpylococcus epidermidis, Propionibacterium acnes  

scholarly journals Penapisan Fitokimia, Kadar Kurkuminoid dan Aktivitas Antibakteri Temu Hitam (Curcuma aeruginosa (Christm) Roscoe.), Temu Putih (Curcuma zedoaria Roxb.) dan Temulawak (Curcuma xanthorrhiza Roxb.)

2021 ◽  
Vol 4 (1) ◽  
pp. 57
Author(s):  
Lia Marliani ◽  
Ika Kurnia Sukmawati ◽  
Dadang Juanda ◽  
Elmadhita Anjani ◽  
Ira Anggraeni
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Staphylococcus Epidermidis ◽  
Phytochemical Screening ◽  
Curcuma Zedoaria ◽  
Propionibacterium Acne ◽  
Microdilution Method ◽  
Turmeric Extract ◽  
Curcuma Xanthorrhiza

The content of secondary metabolites in the rhizome of the Curcuma genus such as Black turmeric (Curcuma aeruginosa Roxb.), White turmeric (Curcuma zedoaria (Christm.) Roscoe) and Java turmeric (Curcuma xanthorrhiza Roxb.) play a role in various pharmacological activities. One of them is the content of the curcuminoid compounds which have been proved to have antibacterial activity. This study aims to screen the content of secondary metabolite compounds, determine curcuminoid content and verify the antibacterial activity of the extracts of Black turmeric (Curcuma aeruginosa Roxb.), White turmeric (Curcuma zedoaria (Christm.) Roscoe) and Java turmeric (Curcuma xanthorrhiza Roxb.). Extraction was carried out by the maceration method using 95% ethanol as solvent. Phytochemical screening was tested for the content of alkaloid, polyphenols, flavonoids, quinones, tannins, saponins, and steroids/ triterpenoids compounds. Determination of curcuminoid content by UV-Vis Spectrophotometry method. The antibacterial activity test was carried out by the microdilution method against Staphylococcus aureus, Staphylococcus epidermidis, and Propionibacterium acne bacteria. The results of phytochemical screening showed that the three extracts contained polyphenols and flavonoids. Quinone compounds are only contained in the extract of Black turmeric and Java turmeric. Saponin compounds were only detected in Black turmeric and White turmeric  extracts. Meanwhile, steroid/ triterpenoid compounds were detected in the extract of White turmeric and Java turmeric. The results of curcuminoid content determination on the three extracts showed that the Java turmeric extract had the highest content of curcuminoids (16.07 ± 0.023 mg CE/g extract). The results of the antibacterial test showed the strongest activity of the three test samples shown by Java turmeric extract with a minimum inhibitory concentration (MIC) value of 64 mg/mL against Staphylococcus aureus bacteria; 256 mg/mL against Staphylococcus epidermidis bacteria; and 32 mg/mL against the Propionibacterium acne bacteria. These results indicate that Java turmeric extract is more active against Propionibacterium acne bacteria.

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