scholarly journals Salinity Stress Mitigation by Foliar Feeding of Salicylic Acid on Maize (Zea Mays L.) grown under Hydroponic Culture

2021 ◽  
Vol 8 (4) ◽  
pp. 67-77
Author(s):  
Zubair Aslam ◽  
Ali Ahmad ◽  
Anser Ali ◽  
Alam Sher ◽  
Muhammad Sarwar
Keyword(s):  
Salicylic Acid ◽  
Salinity Stress ◽  
Positive Impact ◽  
Morphological Attributes ◽  
Saline Environment ◽  
Exogenous Application ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Physiological And Biochemical ◽  
Research Analysis

Salicylic acid (SA) is mainly associated with the regulation of plant growth particularly in salinity stress conditions and this signaling molecule exists in pivotal parts of plant. The objective of this research analysis was to overcome stress condition by distinct foliar treatments of Salicylic acid, for instance, 0 and 100 mM. Foliar applications of SA on maize were rendered on 10 days later than transplanting under a hydroponics experiment. Plants were grown under non-saline (S0= 0 mM NaCl) and saline (S1= 100 mM NaCl) conditions. Evaluation of biochemical, physiological and morphological attributes of maize was rendered after harvesting of plants. The experimental layout of Completely Randomized Design (CRD) under a factorial arrangement with three replications of each treatment was assigned for this study. According to our results it was confirmed that cultivation of maize under saline condition reduced the morphological, physiological and biochemical attributes of plant. However, exogenous application of SA on maize had a positive impact on the above mentioned traits under presence and absence of saline environment. Finally, it was justified that exogenous application of variable concentration of SA significantly improves whole parameters of maize cultivar.

scholarly journals SOMACLONAL PUTATIVE MUTANTS OF RICE TOLERANT TO SALINITY

2018 ◽  
Vol 19 (2) ◽  
pp. 67
Author(s):  
Rossa Yunita ◽  
Nurul Khumaida ◽  
Didy Sopandie ◽  
Ika Mariska
Keyword(s):  
Salinity Stress ◽  
Gamma Ray ◽  
Mutation Induction ◽  
Width Ratio ◽  
Growth Media ◽  
Randomized Design ◽  
Length Width ◽  
Completely Randomized Design ◽  
Mg Content

<p class="abstrakinggris">Soil salinity could significantly reduce rice yield, therefore, varieties tolerant to salinity are urgent to be developed. Mutation induction could be used to create rice mutants tolerant to salinity. The study aimed to evaluate the tolerance of somaclonal mutants of rice to NaCl salinity in a greenhouse condition and characterize their tolerance mechanism. A total of 45 putative mutants were generated by a gamma ray mutation induction followed with <em>in vitro</em> selection in the growth media containing different NaCl concentrations in the greenhouse experiment. The study consisted of two-factor treatments, namely three levels of NaCl concentrations and 45 rice mutants suspected to be tolerant to salinity, arranged in a completely randomized design. Proline, cations (K, Na, Ca, and Mg) content, and stomata density were evaluated.  The results showed that eight mutants were tolerant to 150 mM NaCl, namely CH30, CH-4-2, II-13-42, II-13-7, II-13-10, II-13-13, II-13- 2, and IA-3-21. These tolerant mutants had a higher Na content compared to the check parent. The tolerant mutants had a high proline content, lower Na, and stable K, Mg and Ca cations as well as had a greater number of stomata and higher stomata length-width ratio. Some of the identified tolerant mutants demonstrated the tolerant mechanism against salinity stress. Further studies are required to evaluate these tolerant mutants in the field conditions under salinity stress.</p>

scholarly journals Motility, Viability, Spermatozoa Abnormality, and pH of Bali Cattle Semen in Another-Yellow Water Driller Stored in a Different Time

2021 ◽  
Vol 3 (2) ◽  
pp. 76-90
Author(s):  
Fransiskus X. Manehat ◽  
Agustinus Agung Dethan ◽  
Paulus Klau Tahuk
Keyword(s):  
Positive Impact ◽  
Egg Yolk ◽  
Sugarcane Juice ◽  
Spermatozoa Motility ◽  
Average Value ◽  
Cane Juice ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Bali Cattle

This study aims to determine the quality of Bali cattle spermatozoa diluted using sugarcane juice-egg yolk. This research was carried out in the stables and Laboratory of the Faculty of Agriculture, University of Timor in August-September 2020. Semen was collected from an adult male bali cattle, aged ± 4.5 years in healthy condition. The method used in this study is an experimental method using a completely randomized design consisting of 4 treatments and 5 replications. Each treatment was T1: 0.075 ml semen + 0.3 ml of egg yolk sugarcane juice diluent and stored for 24 hours, T2: semen 0.075 ml + 0.3 ml of diluent for egg yolk cane juice and stored for 48 hours, T3: semen 0.075 ml + 0.3 ml of diluent for egg yolk sugarcane juice and stored for 72 hours, T4: semen 0.075 ml + 0 ,3 ml of egg yolk sugarcane juice diluent and stored for 96 hours. The variables measured were individual motility, viability, spermatozoa abnormalities, and semen pH. The results showed that the T4 treatment on a shelf life of 96 hours still showed a good average value with individual spermatozoa motility values of 45%, spermatozoa viability of 77.3%, spermatozoa abnormalities of 12.5% and semen pH 6.6. It can be concluded that the use of cane juice-egg yolk diluent has a positive impact because it is able to maintain the viability of spermatozoa for 96 hours.    

scholarly journals Variation in morphological attributes and yield of tomato cultivars

2016 ◽  
Vol 3 (2) ◽  
pp. 287-294
Author(s):  
Amit Malaker ◽  
AKM Zakir Hossain ◽  
Tahmina Akter ◽  
Md Shariful Hasan Khan
Keyword(s):  
Agricultural Research ◽  
Yield Attributes ◽  
Morphological Attributes ◽  
Tomato Yield ◽  
Randomized Design ◽  
Fruit Maturity ◽  
Tomato Cultivars ◽  
Completely Randomized Design ◽  
Tomato Genotypes ◽  
Number Of Branches

A pot experiment was carried out in the grill house of the Department of Seed Science and Technology, Bangladesh Agricultural University (BAU), Mymensingh during the period of mid December 2014 to mid April 2015. The experiment conducted with five treatments (five tomato genotypes) and employed in Completely Randomized Design (CRD) with four replications. Of the five genotypes two were obtained Japan (Pasta and Mimi) and the rest three from Bangladesh Agricultural Research Institute (BARI). Genetic variations appeared for morphological and phenological characters and yield attributes in the five tomato genotypes. Taller plants with higher number of branches were observed in the Japanese genotype (Mimi) while number of days required to initiate flower and fruit initiation and fruit maturity were shorter in BARI tomato 12 and BARI tomato 14. Flower and fruit productions plant-1 was higher in BARI released tomato cultivars, therefore, the tomato yield was also higher in those cultivars.Res. Agric., Livest. Fish.3(2): 287-294, August 2016

scholarly journals Effect of gibberellic acid, stratification and salinity on seed germination of Echinacea purpurea cv. Magnus

2015 ◽  
Vol 61 (3) ◽  
pp. 13-22 ◽  
Author(s):  
Shamila Yadolahi Zadeh ◽  
Ali Akbar Ramin ◽  
Bahram Baninasab
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Salinity Stress ◽  
Echinacea Purpurea ◽  
Purple Coneflower ◽  
Randomized Design ◽  
Salinity Levels ◽  
The Mean ◽  
Completely Randomized Design ◽  
Mean Time

SummaryThis study was conducted in order to determine the appropriate treatment for breaking dormancy and the effect of salinity on seed germination of purple coneflower (Echinacea purpureacv. Magnus), in two separate experiments. In the first experiment, five levels of gibberellic acid (GA3) (0, 250, 500, 1000, and 1500 mg×L−1) with four levels of cold moist stratification period of seeds at 5°C (0, 5, 10 and 15 days) were launched. A factorial experiment was conducted in a completely randomized design with four replications. The statistical analysis showed that concentration of 250 mg×L−1GA3with 10 days of cold moist chilling significantly increased the percentage of germination of normal seedlings and reduced the mean time of germination. In the second experiment, the seeds were chilled for 10 days at 5°C and half of them treated with 250 mg×L−1GA3for 24 hours. The seeds treated with GA3, and those non-treated were subjected to NaCl for salinity stress. The experiment was conducted using five salinity levels (0, 20, 40, 60 and 80 mM NaCl) in four replications in a completely randomized design. The results showed that purple coneflower is highly sensitive to salinity in the germination stage. The results also showed that by increasing salinity levels, the percentage of germination and normal seedlings significantly decreased and the mean time to germination increased, compared to the control treatment. But the seeds treated with GA3showed higher viability and better performance under salinity stress condition.

scholarly journals IMUNITAS UDANG VANNAME (Litopenaeus vannamei) YANG DIBERI PAKAN TAMBAHAN DAUN KASEMBUKAN (Paederia foetida Linn.)

2020 ◽  
Vol 12 (2) ◽  
pp. 201-206
Author(s):  
I Ismawati ◽  
R. Amilia Destryana ◽  
Nailiy Huzaimah
Keyword(s):  
Litopenaeus Vannamei ◽  
Positive Impact ◽  
Survival Ratio ◽  
Crop Failure ◽  
Randomized Design ◽  
Shrimp Feed ◽  
Paederia Foetida ◽  
Effect Of Treatment ◽  
Medicinal Properties ◽  
Completely Randomized Design

ABSTRACTOne of the efforts to prevent crop failure in vanname shrimp (Litopenaeus vannamei) can be done by increasing immunity in vanname shrimp. Increased shrimp immunity by chemical means has a weakness that can create resistance to bacteria. To overcome the positive impact of the use of antibiotics in preventing infection in vanname shrimp by utilizing a natural ingredient with medicinal properties namely kasembukan (Paederi foetida Linn.) The research aims to determine the immunity of vanname shrimp and survival ratio. The  research used a Completely Randomized Design with the treatment of paederia leaf concentration added to commercial shrimp feed, namely A1; A2; A3; A4; A5 (0%; 10%; 20%; 40%; 80%). Observations were made on day 0, day 10, day 20 and day 30. The results of research using ANOVA showed that there was an effect of treatment on immune vanname shrimp based on total vanname shrimp hemocytes. Duncon's further tests showed that the treatment of adding 10% and 20% of the paederia leaves had the same effect while the addition of 0%, 40% and 80% of the paederia leaves had a reduced effect on the total haemocyte of vanname shrimp. The survival ratio (SR) of vanname shrimp for each treatment for 30 days were A1: 80%, A2: 100%, A3: 90%, A4: 91% and A5: 96%.Keywords: Immunity, feed, Litopenaus vannamei, Paederia foetida L.ABSTRAKUpaya dalam mencegah kegagalan panen pada budidaya udang vanname (Litopenaeus vannamei) salah satunya dapat dilakukan dengan cara meningkatkan imunitas pada udang vanname. Peningkatan imunitas udang dengan cara kimia memiliki kelemahan yaitu dapat membuat resistensi pada bakteri. Untuk menanggulangi dampak positif penggunaan antibiotik dalam mencegah infeksi pada udang vanname yaitu dengan cara memanfaatkan bahan alami berkasiat obat yaitu kasembukan (Paederi foetida Linn.) Penelitian bertujuan untuk mengetahui imunitas udang vanname serta kelulus hidupan udang yang diberi pakan tambahan daun ksembukan sebagai imunostimulan. Rancangan penelitian menggunakan Rancangan Acak Lengkap dengan perlakuan konsentrasi tepung daun kasembukan yang ditambahkann pada pakan udang komersil yaitu A1; A2; A3; A4; A5 (0%; 10%; 20%; 40%; 80%). Pengamatan dilakukan pada hari ke 0, hari ke 10, hari ke 20 dan hari ke 30. Hasil penelitian menggunakan ANOVA menunjukkan terdapat pengaruh perlakuan terhadap imun udang vanname berdasarkan total hemosit udang vanname. Uji lanjut Duncon menunjukkan bahwa perlakuan penambahan 10% dan 20% daun kasembukan memiliki pengaruh yang sama sedangkan penambahan 0%, 40% dan 80% daun kasembukan memiliki pengaruh yang bereda terhadap total hemosit udang vanname. Kelulus kehidupan udang vanname untuk masing-masing perlakuan selama 30 hari yaitu A1:80%, A2:100%, A3:90%, A4:91% dan A5:96%.Kata Kunci: Imunitas, kasembukan, pakan, udang vanname

scholarly journals TiO2 nanoparticles and salinity stress in relation to artemisinin production and ADS and DBR2 expression in Artemisia absinthium L.

2022 ◽  
Vol 82 ◽  
Author(s):  
S. Shariatzadeh Bami ◽  
R. A. Khavari-Nejad ◽  
A. M. Ahadi ◽  
Z. Rezayatmand
Keyword(s):  
Gene Expression ◽  
Salinity Stress ◽  
Biosynthetic Pathway ◽  
Gene Expressions ◽  
Artemisia Absinthium ◽  
Randomized Design ◽  
Medicinal Properties ◽  
Completely Randomized Design ◽  
Different Levels ◽  
Relative Gene

Abstract Artemisia absinthium L. is an important herb that is widely cultivated in different parts of the world for its medicinal properties. The present study evaluated the effects of four concentrations of nanoparticles treatment (0, 10, 20 and 30 mg L-1) and NaCl salinity stress (0, 50, 100 and 150 mM NaCl) and their interactions with respect to the expression of two key genes, i.e. DBR2 and ADS, in the biosynthesis pathway of artemisinin in A. absinthium. Total RNA was extracted and a relative gene expression analysis was carried out using Real-Time PCR. The amount of artemisinin was also determined by HPLC. All the experiments were performed as factorial in a completely randomized design in three replications. The results revealed that salinity stress and nanoparticles treatment and their interaction affected the expressions of these genes significantly. The highest levels of ADS gene expression were observed in the 30 mg L-1 nanoparticles–treated plants in the presence of 150 mM salinity stress and the lowest levels in the 10 mg L-1 nanoparticles–treated plants under 50 mM salinity stress. The maximum DBR2 gene expression was recorded in the 10 mg L-1 nanoparticles–treated plants in the absence of salinity stress and the minimum expression in the 100 mM salinity-stressed plants in the absence of nanoparticles treatment. Moreover, the smallest amounts of artemisinin were observed in the 150 mM salinity-stressed plants in the absence of nanoparticles and the highest amounts in the 30 mg L-1 nanoparticles–treated plants. The maximum amounts of artemisinin and ADS gene expression were reported from the plants in the same nanoparticles treatment and salinity stress conditions. In this regard, the amount of artemisinin was decreased by half in the plants containing the highest DBR2 gene expression. Meanwhile, no significant correlation was observed between these gene expressions and the artemisinin amount in the other nanoparticles–treated plants under different levels of salinity stress. The biosynthetic pathway of secondary metabolites appears to be very complex and dose not directly dependent on these gene expressions.

scholarly journals Growth, Yield and Resistance Responses of Three Cultivars on True Seed Shallots to Twisted Disease with Salicylic Acid Application

2019 ◽  
Vol 35 (1) ◽  
pp. 1
Author(s):  
Rachmanto Bambang Wijoyo ◽  
Endang Sulistyaningsih ◽  
Arif Wibowo
Keyword(s):  
Salicylic Acid ◽  
Disease Management ◽  
Growth Yield ◽  
True Seed ◽  
Randomized Design ◽  
Two Factors ◽  
Completely Randomized Design ◽  
Spray Treatment

Twisted disease is one of the main diseases on shallot caused by <em>Fusarium </em>sp<em>.</em> One method to increase the resistance of shallot cultivars to the twisted disease can be performed by using True Seed Shallot (TSS) combining with a salicylic acid application. TSS is known as free pathogen. The objective of the research was to examine the effects of a salicylic acid application to the growth, yield and resistance responses of TSS on Tuktuk, Sanren and Lokananta cultivars to the twisted disease. The research was arranged in factorial Completely Randomized Design (CRD), consisting of two factors with three replications. The first factor included Tuktuk, Sanren and Lokananta cultivars and the second factor comprised salicylic acid applications (without salicylic acid and inoculation, without salicylic acid with inoculation, immersion treatment of salicylic acid with inoculation, spray treatment with inoculation as well as immersion and spray treatment with inoculation). The results showed that the application of salicylic acid in different treatments had the same effects on the growth, yield and resistance of TSS. An interesting result is found, in which the application of salicylic acid by immersing and spraying could reduce the incidence of twisted disease by 12.5% in TSS. Therefore, the application of salicylic acid by immersing and spraying can be one of the recommended twisted disease management.

scholarly journals Physiological potential of piquin pepper seeds in response to pregermination treatments

2021 ◽  
Vol 45 ◽  
Author(s):  
Miguel Ángel Cano-González ◽  
Benjamín Abraham Ayil-Gutiérrez ◽  
Rafael Delgado-Martínez ◽  
Eduardo Osorio-Hernández ◽  
José Antonio Rangel-Lucio ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Salicylic Acid ◽  
Growth Regulators ◽  
Germination Percentage ◽  
Wild Plant ◽  
Naphthalene Acetic Acid ◽  
Distilled Water ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Pharmaceutical Properties

ABSTRACT Wild piquin pepper fruits are consumed and traded because of their flavor, nutritional and pharmaceutical properties. The heterogeneous germination of its seeds has caused pregermination treatments to be unstandardized. Because of that, our goal was to evaluate pregermination treatments of piquin pepper seeds from a wild plant from the municipality of Victoria, Tamaulipas, Mexico, consisting of immersion for different periods (2, 24, 48 h) in solutions with variable concentrations of the growth regulators naphthalene acetic acid (NAA; 0.1, 1.0, 2.5 mM), salicylic acid (SA; 1.0, 5.0, 10 mM), gibberellic acid (GA3, 1.0, 5.0, 10 mM), indole-3-butyric acid (IBA; 1.0, 2.0, 3.0 mM), the commercial product Biozyme (Bioz) containing minerals and growth regulators (Bioz; 0.5, 1, 2%), and sulfuric acid (H2SO4; 2, 5, 10%) in a completely randomized design, using distilled water as the control. For each treatment, we measured the imbibition ratio, germination percentage, time to obtain 50% germination (T50), and coefficient of variation of germination time (CVt). Our results showed that the imbibition was similar in the control and the treatments, but it differed between treatments. The highest imbibition recorded in 48 h was 87%. The highest germination percentage (79.2%) was achieved with 2 h immersion in 2% Bioz, followed by 1 mM NAA (62%) and 5 mM SA (56%). T50 and CVt were 10-26 d and 0-39%, respectively. According to our results, we concluded that the pregermination treatments we assayed improved the physiological potential for the germination of piquin pepper seeds.

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