scholarly journals TiO2 nanoparticles and salinity stress in relation to artemisinin production and ADS and DBR2 expression in Artemisia absinthium L.

2022 ◽  
Vol 82 ◽  
Author(s):  
S. Shariatzadeh Bami ◽  
R. A. Khavari-Nejad ◽  
A. M. Ahadi ◽  
Z. Rezayatmand
Keyword(s):  
Gene Expression ◽  
Salinity Stress ◽  
Biosynthetic Pathway ◽  
Gene Expressions ◽  
Artemisia Absinthium ◽  
Randomized Design ◽  
Medicinal Properties ◽  
Completely Randomized Design ◽  
Different Levels ◽  
Relative Gene

Abstract Artemisia absinthium L. is an important herb that is widely cultivated in different parts of the world for its medicinal properties. The present study evaluated the effects of four concentrations of nanoparticles treatment (0, 10, 20 and 30 mg L-1) and NaCl salinity stress (0, 50, 100 and 150 mM NaCl) and their interactions with respect to the expression of two key genes, i.e. DBR2 and ADS, in the biosynthesis pathway of artemisinin in A. absinthium. Total RNA was extracted and a relative gene expression analysis was carried out using Real-Time PCR. The amount of artemisinin was also determined by HPLC. All the experiments were performed as factorial in a completely randomized design in three replications. The results revealed that salinity stress and nanoparticles treatment and their interaction affected the expressions of these genes significantly. The highest levels of ADS gene expression were observed in the 30 mg L-1 nanoparticles–treated plants in the presence of 150 mM salinity stress and the lowest levels in the 10 mg L-1 nanoparticles–treated plants under 50 mM salinity stress. The maximum DBR2 gene expression was recorded in the 10 mg L-1 nanoparticles–treated plants in the absence of salinity stress and the minimum expression in the 100 mM salinity-stressed plants in the absence of nanoparticles treatment. Moreover, the smallest amounts of artemisinin were observed in the 150 mM salinity-stressed plants in the absence of nanoparticles and the highest amounts in the 30 mg L-1 nanoparticles–treated plants. The maximum amounts of artemisinin and ADS gene expression were reported from the plants in the same nanoparticles treatment and salinity stress conditions. In this regard, the amount of artemisinin was decreased by half in the plants containing the highest DBR2 gene expression. Meanwhile, no significant correlation was observed between these gene expressions and the artemisinin amount in the other nanoparticles–treated plants under different levels of salinity stress. The biosynthetic pathway of secondary metabolites appears to be very complex and dose not directly dependent on these gene expressions.

scholarly journals PTEN-dependent changes of gene expressions in human prostate tumors

2018 ◽  
Vol 22 ◽  
pp. 113-119
Author(s):  
G. V. Gerashchenko ◽  
O. V. Grygoruk ◽  
L. V. Mevs ◽  
V. I. Kashuba
Keyword(s):  
Gene Expression ◽  
Human Prostate ◽  
Benign Tumors ◽  
Relative Gene Expression ◽  
Gene Expressions ◽  
Prostate Tumors ◽  
Normal Tissues ◽  
Histological Characteristics ◽  
Different Levels ◽  
Relative Gene

Aim. To determine the levels of PTEN relative expression (RE) and the possible effects of its loss on some tumor-associated genes and histological characteristics of human prostate tumors. Methods. PTEN RE and 46 genes were identified in 37 pairs of adenocarcinoma and conventionally normal tissues (CNT) and 20 adenomas by real time PCR. Results. We are shown PTEN RE decreasing in the adenocarcinoma group as well as in CNTs compared with adenomas. 54 % of adenocarcinomas, 48.6 % of CNT and 25 % of adenomas were detected with low PTEN RE. We have found 9 of 46 differentially expressed genes in groups with different levels of PTEN RE (ESR1, GCR, KRT18, MMP2, MMP9, SRD5A2, VIM, PCA3, HOTAIR). It was revealed some histological characteristics correlated with PTEN RE in prostate adenocarcinomas. Conclusions. PTEN aberrations in prostate tumors indicate that PTEN associated changes already have been occurred at the benign tumors. We have shown some tumor histological characteristics associated with PTEN expression changes.Keywords: prostate tumors, relative gene expression, PTEN, EMT, histological characteristics.

Herba Reudeuep With Modification of Heating In Virgin Cocanut Oil

2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Wahyuzan Wahyuzan ◽  
Lukmanul Hakim ◽  
Rahmat Afrizal ◽  
Asmeri Lamona ◽  
Khairuni Khairuni ◽  
...  
Keyword(s):  
Modern Medicine ◽  
Heating Time ◽  
Agricultural Product ◽  
Average Yield ◽  
Herbal Products ◽  
The Public ◽  
Randomized Design ◽  
Herbal Plants ◽  
Medicinal Properties ◽  
Completely Randomized Design

Traditional medicine in Indonesia has been known by the public before formal health services using modern medicine. Indonesia has many type of herbal plants which have medicinal properties including dadap (Erythrina orientalis). This study aims to utilized dadap herbal liniment by modifying heating in Vigin Cocanut Oil. The study conducted in the laboratory of Agricultural Product Processing using a Factorial Completely Randomized Design (CRD). The first factor is the addition of 250 ml (M1), 375 ml (m2), and 400 ml (M3) Virgin Cocanut Oil treatment. The second factor is the heating tim of 5 minutes (L1), 10 minutes (L2), and 15 minutes (L3). Observation variable consisted of yield, Specific gravity, and organoleptic test of aroma and colored. The result showed an average yield of 87.70-91.88%. The highest yield was found in the treatment of adding 400 ml Virgin Cocanut Oil and 15 minutes of heating time. The highest type of weight was found in the treatment of addition of 325 ml Virgin Cocanut Oil and 5 minutes heating time. Based on the organoleptic scent test, the everage penalist gives a like answer. Organoleptic colors of reudeuep herbal products is according to SNI number 06-23-2006.

scholarly journals Nutrient and inorganic solute (Na+ and Cl-) content in green onion plants under hydroponic cultivation using brackish water

2020 ◽  
Vol 44 ◽  
Author(s):  
Carlos Donato da Silva Souza ◽  
Geronimo Ferreira da Silva ◽  
Sirleide Maria de Menezes ◽  
José Edson Florentino de Morais ◽  
José Amilton Santos Júnior ◽  
...  
Keyword(s):  
Nutrient Solution ◽  
Brackish Water ◽  
Hydroponic System ◽  
Brackish Waters ◽  
Randomized Design ◽  
Hydroponic Cultivation ◽  
Salinity Levels ◽  
Completely Randomized Design ◽  
Harmful Effects ◽  
Different Levels

ABSTRACT Cultivation using brackish waters can result in nutritional and metabolic imbalances in several plant species, consequently reducing the production of dry matter (DM) and accumulation of toxic ions (Na+ and/or Cl-) in plants. We evaluated the DM production, and nutrient and inorganic solute (Na+ and Cl-) content in green onion plants (cv. Todo Ano Evergreen - Nebuka) under different levels of nutrient solution salinity in combination with circulation frequencies of this solution. Two experiments were conducted in a hydroponic system, using a completely randomized design, in a 6 × 2 factorial scheme, with five replicates: six levels of nutrient solution salinity (1.5, 3.0, 4.5, 6.0, 7.5, and 9.0 dS m-1) and two solution circulation frequencies (twice and thrice a day). In Experiment I, the evapotranspired depth was replaced using brackish water that was used to prepare each of the salinity levels (used exclusively), whereas in Experiment II, brackish water was used only to prepare each of the salinity levels and public water was used (electrical conductivity [ECw] = 0.12 dS m-1) for replacement in all treatments. The increase in the nutrient solution salinity reduced the production of DM and accumulation of nutrients; the reductions were more pronounced when brackish waters were used exclusively (Experiment I). However, the circulation of solutions thrice a day resulted in the harmful effects of the salinity effect. Replacing the evapotranspirated blade with water supply (Experiment II) mitigated the deleterious effects of salinity. Moreover, three circulations of the nutrient solution daily resulted in lower accumulation of inorganic Na+ and Cl- solutes and increased accumulation of nutrients N, P, K+, Ca2+, Mg2+, and S in the culture.

scholarly journals The quality properties, thiobarbituric acid (TBA) values and microstructure of chicken sausage with local red beetroot powder

Food Research ◽  
2021 ◽  
Vol 5 (S2) ◽  
pp. 113-119
Author(s):  
W. Swastike ◽  
E. Suryanto ◽  
Rusman ◽  
C. Hanim ◽  
Jamhari ◽  
...  
Keyword(s):  
Peroxide Value ◽  
Room Temperature ◽  
Thiobarbituric Acid ◽  
Quality Properties ◽  
Randomized Design ◽  
Powder Addition ◽  
Completely Randomized Design ◽  
Different Levels ◽  
Temperature Storage

This research was aimed to determine the quality properties, the microstructure of chicken sausage and Thiobarbituric acid (TBA) values with locally Indonesia red beetroot powder. The main ingredients of chicken sausage-making in this research were broiler chicken, filler, binder, beetroot powder, and spices. Red beetroot powder function as a filler was substituted tapioca starch in chicken sausage batter in three different levels. The combination of red beetroot powder with level 0, 1.0, 2.0 and 3.0% of total batter and shelf life at room temperature for 0, 1, 2 and 3 days. Each treatment consisted of five replications. The variables observed using quality properties (moisture, ash, fat, protein, crude fiber and calorie), microstructure and peroxide value of chicken sausage. The data of quality properties and peroxide value were analyzed by using one-way analysis (ANOVA) of Completely Randomized Design. The differences between means were analyzed by Duncan's New Multiple Ranges Test. The data of microstructure was analyzed by descriptive analyses. The moisture, protein, fat and ash contents for chicken sausages were significantly different (p<0.05). The chicken sausage with 2% substitution of beet powder produced chicken sausages with a high protein content of 14.77±0.02% while a low-fat content is 0.42±0.01%. Thiobarbituric acid (TBA) values of chicken sausages increased throughout the three days of room temperature storage (38°C). Chicken sausage formulated with red beetroot powder showed a significantly lower TBA value compared to the samples without red beetroot powder (p<0.05). In conclusion, a higher level of beetroot powder will improve the quality of chicken sausage and also the microstructure. The best level of beetroot powder addition was 2.0%. The addition of beetroot powder able to maintain fresh sausage conditions up to 2 days of storage at room temperature.

scholarly journals Physicochemical, Microbiological and Sensory Properties of Fermented Whey using Kombucha Inoculum

2019 ◽  
Vol 43 (1) ◽  
Author(s):  
Fitri Suciati ◽  
Nurliyani Nurliyani ◽  
Indratiningsih Indratiningsih
Keyword(s):  
Green Tea ◽  
Black Tea ◽  
Acetic Acid Bacteria ◽  
Solid Content ◽  
Sensory Properties ◽  
Whey Protein Concentrate ◽  
Plate Count ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Different Levels

This research aimed to determine physicochemical, microbiological and sensory properties of fermented whey using kombucha inoculum. The material used were kombucha, black tea, green tea, and whey protein concentrate. The research used factorial completely randomized design. The first factor was kombucha inoculum using different mediums (black and green tea) at different levels (5; 10; 15; dan 20%) as a second factor. The whey was fermented at 37oC for 39 hours. Research showed the use of kombucha inoculum using different medium (black and green tea) at different levels (5; 10; 15; dan 20%) did not affect (p>0.05) on microbiological properties (the number of Total Plate Count, total lactic acid bacteria, total acetic acid bacteria, and total yeast), dissolved protein content, lactose content, viscosity and acceptability. Black and green tea kombucha inoculum can be used in whey fermentation. Black tea kombucha fermented whey with 20% level addition has the best solid content. Relatively, kombucha fermented whey is quite acceptable.

scholarly journals Economic analysis of the finishing of lambs under confinement conditions using licuri cake ( Syagrus coronata Mart. Becc.)

2019 ◽  
Vol 20 ◽  
Author(s):  
Jonival Barreto COSTA ◽  
Ronaldo Lopes OLIVEIRA ◽  
Thadeu Mariniello SILVA ◽  
Ossival Lolato RIBEIRO ◽  
Rebeca Dantas Xavier RIBEIRO ◽  
...  
Keyword(s):  
Soybean Meal ◽  
Dry Matter ◽  
Randomized Design ◽  
Economic Parameters ◽  
Completely Randomized Design ◽  
The Impact ◽  
Operation Cost ◽  
Different Levels ◽  
Ground Corn ◽  
Corn Grain

SUMMARY The present study endeavors to determine the impact of the inclusion of licuri tart in the diet of finishing lambs, Santa Inês mestizos, by evaluating the economic parameters. For this purpose, a total of 44 lambs, including both uncastrated as well as vaccinated and wormed lambs, with an average age of six months and a mean body weight of 21.2 kg ± 2.7 kg, were employed. Initially, the lambs were fed on the diet which consisted of 40% Tifton-85 hay and 60% of a concentrated mixture, mainly composed of ground corn grain, soybean meal, mineral premix, being further followed by the inclusion of the licuri cake at four different levels (a) 0.0, (b) 8.0,(c) 16.0, and (d) 24% with respect to the dry matter. Principally, the soybean meal and milled corn were replaced by the licuri cake with these levels, thereby constituting four treatments and 11 replicates (11 lambs per treatment) in a completely randomized design. Further, for subjecting the lambs to the above mentioned four different levels of treatment, the lambs were confined for 70 days. The analysis of various economic parameters revealed that upon including the licuri cake up to the level of 24%, the operation cost decreases, which in turn results in higher profitability. However, its cost at the time of use will eventually determine its use for feeding lambs, because of the possible price variations of the ingredients used to formulate the diets along with the model of production of the rural property.

scholarly journals Changes in Liver Gene Expression and Plasma Concentration of Rbp4, Fetuin-A, and Fgf21 in Sprague-Dawley Rats Subjected to Different Dietary Interventions and Bariatric Surgery

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Dominika Stygar ◽  
Wojciech Pigłowski ◽  
Elżbieta Chełmecka ◽  
Bronisława Skrzep-Poloczek ◽  
Tomasz Sawczyn ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dietary Patterns ◽  
Plasma Levels ◽  
Control Diet ◽  
Retinol Binding Protein ◽  
Fibroblast Growth Factor 21 ◽  
Relative Gene Expression ◽  
Gene Expressions ◽  
Fetuin A ◽  
Relative Gene

Purpose. To study the effect of duodenal-jejunal omega switch (DJOS) in combination with different dietary patterns on the retinol-binding protein (RBP4), fetuin-A, and fibroblast growth factor 21 (FGF21) plasma levels and their hepatic gene expressions in rats. Methods. A high-fat diet (HF) was given to 28 rats and 28 more were fed with a control diet (CD) for 2 months. After that, half of each group underwent either DJOS or SHAM surgery. For the next 2 months, half of the animals in each operation group were kept on the same diet as before and half of them had the diet changed. After 16 weeks of the experiment RBP4, fetuin-A, and FGF21 plasma levels as well as liver Rbp4, Ahsg, and Fgf21 gene expressions were measured. Results. DJOS had a reductive impact on plasma levels of RBP4, fetuin-A, and FGF21 and Rbp4, Ahsg, and Fgf21 relative gene expression in the liver when compared to SHAM. The HF/HF group expressed significantly higher RBP4 and fetuin-A plasma levels in comparison to the control. The HF diet used before and/or after surgery led to upregulation of Rbp4, Ahsg, and Fgf21 relative gene expression. The lowest levels of analyzed parameters were observed in the CD/CD group. Conclusions. The efficiency of DJOS surgery, measured by hepatokines’ plasma levels and their gene expressions in the liver, depends on the type of diet applied before and after surgery. Manipulation of dietary patterns can lead to marked improvements in metabolic profile after DJOS surgery.

scholarly journals Golden mussel (Limnoperna fortunei) in feed for broiler chicks using tannin as a sequestrant of toxic metals

2017 ◽  
Vol 38 (2) ◽  
pp. 843
Author(s):  
Douglas Fernando Bayerle ◽  
Ricardo Vianna Nunes ◽  
Affonso Celso Gonçalves Junior ◽  
Lucas Wachholz ◽  
Carina Scherer ◽  
...  
Keyword(s):  
Toxic Metals ◽  
Inert Material ◽  
Broiler Chicks ◽  
Feed Conversion ◽  
Randomized Design ◽  
Low Concentrations ◽  
Effect Of Treatment ◽  
Completely Randomized Design ◽  
Golden Mussel ◽  
Different Levels

This study aimed to evaluate the use of wattle tannin as an adsorbent of toxic metals in broilers fed on diets with different levels of replacement of limestone with golden mussel meal. First, we conducted a trial to evaluate the performance of broiler chicks from 1 to 21 days old when fed on wattle tannin. For this we used 720 male broiler chicks that were one day old, in a completely randomized design with six treatments and six replications. The treatments were composed of basal diets with increasing levels of wattle tannin (0, 250, 500, 750, 1000 and 1250 g tonne-1), where the tannin replaced the inert material of the feed. All of the diets were isonutritive and isocaloric. The results showed that 250 g tonne-1 of tannin was detrimental to weight gain, final weight and feed conversion, and the use of 1000 g tonne-1 of wattle tannin, in addition to these effects, caused a drop in consumption of the ration. There was no effect of treatment on morphometric measurements of villi, crypt or the villus:crypt ratio. Next, we conducted a second test to evaluate the performance of broiler chicks from 1 to 21 days of age when fed with increasing levels of replacement (0, 25, 50, 75, 100%) of limestone with golden mussel and even supplementation or not wattle tannin. Five repetitions were used and the birds were housed in a completely randomized design. In diets with the inclusion of tannin, 250 g tonne-1 of wattle tannin was used, which replaced the inert material in the feed. In this test, 1200 male broiler chicks that were one day old were used. The results showed that the golden mussel contains large quantities of calcium, with low concentrations of toxic metals and microbiological contamination within the allowed range. Performance data show that mussel can be used to replace up to 100% of limestone in feed without affecting the variables, but the use of 250 g tonne-1 of wattle tannin affects the performance of chicks. Bone quality was not changed by the treatments. Phosphorus and calcium in the bones remained stable during treatment and only the gray variation showed interaction effects from the use of mussel and wattle tannin.

scholarly journals ANATOMICAL STUDY OF MORINGA LEAF (MORINGA OLEIFERA LAM.) UNDER IRON FERTILIZATION

2020 ◽  
Vol 21 (supplement 1) ◽  
Author(s):  
Jawad Kadhim Obaid Al-Hujayri ◽  
Abdoun H. Alwan ◽  
Neepal Imtair Al-garaawi
Keyword(s):  
Moringa Oleifera ◽  
Foliar Application ◽  
Anatomical Study ◽  
Iron Mineral ◽  
Randomized Design ◽  
The Mean ◽  
Completely Randomized Design ◽  
Normal Epidermis ◽  
Different Levels ◽  
Moringa Oleifera Lam

The effect of different levels of nano- and mineral-Iron and their method of application on anatomical properties of the upper and lower epidermis of Moringa (Moringa oleifera Lam.) leaves was studied. Seeds of Moringa were sown in 15 kgs soil capacity pots during the growing season of 2018/2019 arranged as a factorial experiment within Completely Randomized Design, with three replicates. The factorswere a method of fertilization(soil or foliar application),three levels i.e. 0,180 and 360mg.l-1 of each of mineral and nano-Iron. The total number of experimental units was 2×3×3×3 for method, nano-Iron, mineral-Iron, and replicates respectively.The application method caused an increase in the mean dimensions of normal epidermis cells, stomatoes, and hairs.These traits were increased as Iron levels increased. Where 360 mg.l-1 of both types of Iron gave the highest dimensions. Soil application gave higher values of epidermis dimensions than that obtained from the foliar application. The number of stomatoesincreased with increasing the level of Iron. The number of normal epidermis cells was conversely proportioned with their volume.

scholarly journals SOMACLONAL PUTATIVE MUTANTS OF RICE TOLERANT TO SALINITY

2018 ◽  
Vol 19 (2) ◽  
pp. 67
Author(s):  
Rossa Yunita ◽  
Nurul Khumaida ◽  
Didy Sopandie ◽  
Ika Mariska
Keyword(s):  
Salinity Stress ◽  
Gamma Ray ◽  
Mutation Induction ◽  
Width Ratio ◽  
Growth Media ◽  
Randomized Design ◽  
Length Width ◽  
Completely Randomized Design ◽  
Mg Content

<p class="abstrakinggris">Soil salinity could significantly reduce rice yield, therefore, varieties tolerant to salinity are urgent to be developed. Mutation induction could be used to create rice mutants tolerant to salinity. The study aimed to evaluate the tolerance of somaclonal mutants of rice to NaCl salinity in a greenhouse condition and characterize their tolerance mechanism. A total of 45 putative mutants were generated by a gamma ray mutation induction followed with <em>in vitro</em> selection in the growth media containing different NaCl concentrations in the greenhouse experiment. The study consisted of two-factor treatments, namely three levels of NaCl concentrations and 45 rice mutants suspected to be tolerant to salinity, arranged in a completely randomized design. Proline, cations (K, Na, Ca, and Mg) content, and stomata density were evaluated.  The results showed that eight mutants were tolerant to 150 mM NaCl, namely CH30, CH-4-2, II-13-42, II-13-7, II-13-10, II-13-13, II-13- 2, and IA-3-21. These tolerant mutants had a higher Na content compared to the check parent. The tolerant mutants had a high proline content, lower Na, and stable K, Mg and Ca cations as well as had a greater number of stomata and higher stomata length-width ratio. Some of the identified tolerant mutants demonstrated the tolerant mechanism against salinity stress. Further studies are required to evaluate these tolerant mutants in the field conditions under salinity stress.</p>

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