Comparative analysis of antioxidant and secondary metabolites contents in eight populations of Medicago ciliaris L.
1 ABSTRACT To identify the suitable genetic resources of forage legumes for animal feeding, the characterization of their chemical and biochemical composition is crucial since forage quality is a limiting factor which affects animal health and performance and consequently human health. In this context, this study carried out for the first time the analyses of the antioxidant compounds and the secondary metabolites (total phenols and tannins and saponins) in whole fresh biomass, flowers and stems of eight populations of Medicago ciliaris L (echinus Medicago) collected in different bioclimatic areas of north Tunisia. The analysis of variance showed significant differences between populations for all parameters (P<0.05) except for anthocyanins (P>0.05). Significant correlations were found between carotenoids and chlorophylls a and b (r= 0.59, n=48; P<0.0001) and between lycopene and β-carotene (r=0.81, n=48; P<0.0001). Multivariate analysis showed that populations 306 and 326 had the highest contents of chlorophyll a and carotenoids while Pop3 had the highest content of lycopene, β-carotenes, saponines and total tannins. These populations will be integrated in a breeding program for quality improvement of forage and will serve for grazing of pods and straw in summer and grazing of green biomass in winter and spring accordingly to their richness in antioxidant compounds.