Etudes ethnomédicinale, phytochimie et activité antioxydante de Crateva adansonii DC (Capparidaceae) dans les communes de Cotonou et de Dassa-Zoumè au Benin

Crateva adansonii constitue l’une des espèces prisées au sein de la population béninoise pour ses nombreuses vertus thérapeutiques et nutritionnelles. La présente étude intitulée a été initiée pour garantir et promouvoir une meilleure utilisation de Crateva adansonii dans le traitement des maladies. L’étude ethnomédicinale a été réalisée au moyen des interviews semi structuré auprès des herboristes et des consommateurs de Cotonou et de Dassa. Le screening phytochimique a été effectué par chromatographie sur couche mince (CCM) doublé du dosage spectrophotométrique des métabolites secondaires. Les méthodes FRAP et DPPH ont servi à la détermination du pouvoir antioxydant et la CI 50% a été calculée. Les enquêtés ethnomédicinales ont montré que Crateva adansonii s’utilise au Bénin aussi bien à des fins thérapeutiques, alimentaire que médico-magique. La forme galénique de ses organes les plus utilisées (tiges feuillées) est la décoction par voie orale. La phytochimie a révélé, en plus des principaux groupes phytochimiques (composés phénoliques, composés azotés, composés stéroïdes et terpénoïdes et mucilages), la présence des huiles essentielles, des lignanes et des pigments anthocyaniques. Dans la gamme de concentrations utilisées (0,23 – 30 µg.ml-1), le pouvoir antioxydant de l’extrait varie de 5 – 58% (méthode DPPH) et de 23,01 à 34,06mMolEAA/g (méthode FRAP). Quant au dosage des métabolites secondaires, l’extrait éthanolique de Crateva adansonii renferme respectivement 12,85 mg Eq Cat/100 mg de tannins totaux, 29,64 mg Eq Quer/100 mg de flavonoïdes totaux et 3,51 mg Eq ac. Galic/100 mg de phénoliques totaux. Mignanwandé et al., 2020 Journal of Animal & Plant Sciences (J.Anim.Plant Sci. ISSN 2071-7024) Vol.46 (1): 8071-8089 https://doi.org/10.35759/JAnmPlSci.v46-1.2 8072 ABSTRACT Crateva adansonii is one of the species prized by the Beninese population for its many therapeutic and nutritional benefits. This titled study was initiated to ensure and promote better use of Crateva adansonii in the treatment of diseases. The ethnomedicinal study was carried out through semi-structured interviews with herbalists and consumers in Cotonou and Dassa. The phytochemical screening was carried out by thin layer chromatography (TLC) coupled with the spectrophotometric assay of the secondary metabolites. The FRAP and DPPH methods were used to determine the antioxidant power and the IC 50% was calculated. Ethnomedicinal surveys have shown that Crateva adansonii is used in Benin for therapeutic, nutritional and medico-magical purposes. The dosage form of its most widely used organs (leafy stems) is the oral decoction. Phytochemistry revealed, in addition to the main phytochemical groups (phenolic compounds, nitrogen compounds, steroid and terpenoids compounds and mucilages), the presence of essential oils, lignans and anthocyanin pigments. In the range of concentrations used (0.23 - 30 µg.ml-1), the antioxidant power of the extract varies from 5 - 58% (DPPH method) and from 23.01 to 34.06 mMolEAA / g (FRAP method). As for the determination of secondary metabolites, the ethanolic extract of Crateva adansonii contains respectively 12.85 mg Eq Cat / 100 mg of total tannins, 29.64 mg Eq Quer / 100 mg of total flavonoids and 3.51 mg Eq ac. Galic / 100 mg total phenolics.

Download Full-text

Chemical characterization of ethanolic extract of Butterfly pea flower (Clitoria ternatea)

Food Research ◽

10.26656/fr.2017.5(4).744 ◽

2021 ◽

Vol 5 (4) ◽

pp. 127-134

Author(s):

T.N.M. Tuan Putra ◽

M.K. Zainol ◽

N.S. MohdIsa ◽

N. MohdMaidin

Keyword(s):

Antioxidant Activity ◽

Total Phenolic Content ◽

Phenolic Content ◽

Light Exposure ◽

Ethanolic Extract ◽

Total Phenolic ◽

Antioxidant Power ◽

Clitoria Ternatea ◽

Dpph Method ◽

Butterfly Pea

Clitoria ternatea, commonly known as Butterfly pea flower or Bunga telang, is a tropical flower and notable as one of the most vital sources of polyphenols with high antioxidant capacity. C. ternatea abundantly produces anthocyanin compounds benefiting natural blue colourants. To date, a blue colourant is the most difficult to obtain since the anthocyanins responsible for this colour is the least stable and easily degraded after extraction. Their stability commonly relies on several operational parameters, such as shifted pH value, high temperature, and light exposure. Apart from colour attributes, anthocyanins have also been identified as a source of functional molecules due to antioxidant activity and beneficial health effects such as anti-cancer, anti-obesity, antimicrobial and more. This study aimed to characterize the chemical constituents of the extractable polyphenols from ethanolic extract of C. ternatea quantitatively. The numerical data were evaluated using the Minitab version 18 statistical method. The chemical profiling, including total phenolic content (TPC), total flavonoid content (TFC), total monomeric anthocyanins (TMA) were determined using standard methods. The antioxidant activity was evaluated using DPPH and ABTS methods. The results showed that the ethanolic extract of C. ternatea had 35.7 mg QE/g of TFC, 102.4 mg GAE/g280nm and 28.8 mg GAE/g750nm of TPC and 2.7 CE/g and 2.9 ME/g of TMA. The total phenolic content of C. ternatea showed a good correlation with the antioxidant activity by the DPPH method when analysed using Pearson correlation and showed an increasing trend of antioxidant power with regards to TPC in both assays. In a nutshell, this study contributes to the knowledge of ethanolic extract of C. ternatea. Future research could consider exploring other extraction methods that could enhance the stability of polyphenols particularly the anthocyanins from degradation such as using surfactants.

Download Full-text

SCREENING OF ANTIOXIDANTS, ANTI-AGING AND TYROSINASE INHIBITORY ACTIVITIES OF ETHANOLIC AND ETHYL ACETATE EXTRACTS OF FRUIT FLESH AND FRUIT PEEL LANGSAT (Lansium domesticum Corr) IN VITRO

Majalah Obat Tradisional ◽

10.22146/tradmedj.24342 ◽

2017 ◽

Vol 22 (1) ◽

pp. 63 ◽

Cited By ~ 2

Author(s):

Syamsu Nur ◽

Rumiyati Rumiyati ◽

Endang Lukitaningsih

Keyword(s):

Ethyl Acetate ◽

Colorimetric Method ◽

Ethanolic Extract ◽

Inhibitory Effect ◽

Total Phenolic ◽

Flavonoid Content ◽

Fruit Peel ◽

Antioxidant Power ◽

Fruit Flesh ◽

Dpph Method

This study aims to determine the bioactivity of L.domesticum as cosmetic ingredient. The extraction was prepared by maceration with ethanol 96 % and ethyl acetate to obtain ethanolic extract of fruit flesh (BLE), ethanolic extract of fruit peel (KLE), ethyl acetate extract of fruit flesh (KLE) and fruit peel (KLEA). Then, determination of compounds was conducted by thin layer chromatography (TLC) and analysed of total phenolic and flavonoid content with colorimetric method. The antioxidant capacity was measured using radical scavenging assay of 1,2-diphenyl-2-picrylhydrazyl (DPPH), β-carotene bleaching assay (BCB) and ferric reducing antioxidant power assay (FRAP). The inhibitory effect of skin degradation enzymes (anti-aging) was carried out using elastase and collagenase assay. The tyrosinase inhibitory effect was determined using mushroom tyrosinase. Based on TLC result, extract of L.domesticum containing phenolic, flavonoids, terpenoids and steroids compounds. The largest of total flavonoid content was obtained in KLE (4,76±0,26 %b/b EQ) and phenolic total content in BLEA (6,4±0,15 %b/b EG). Sequence of antioxidant activity measured using DPPH method was BLEA> KLE> BLE> KLEA; while using BCB method and FRAP method were respectively KLEA>BLEA> KLE> BLE; and BLEA> KLE> BLE> KLEA. KLE and KLEA extracts showed the most potent to remaining activity of the enzyme elastase and collagenase. In addition, BLE extract has the higest tyrosinase inhibition than others. Based on these results it can be concluded that the extracts of the fruit flesh and fruit peel of L. domesticum can be used as a cosmetic active ingredient for anti-aging and anti-tyrosinase.

Download Full-text

Antioxidant Activity of Dendrobium lasianthera and Geodorum densiflorum

Journal of Pharmacological Research and Developments ◽

10.46610/jprd.2021.v03i02.004 ◽

2021 ◽

Vol 3 (2) ◽

Author(s):

Eva Susanty Simaremare ◽

Eva Susanty Simaremare

Keyword(s):

Antioxidant Activity ◽

Secondary Metabolites ◽

Ethanolic Extract ◽

Ethanol Extract ◽

Natural Antioxidants ◽

Dpph Method ◽

Ethanolic Extracts

Many antioxidants are obtained from plants that contain secondary metabolic compounds such as alkaloids, flavonoids, saponins, tannins, etc. Thus, the search for plants that are rich in natural antioxidants is conducted continuously, including orchids. Dendrobium lasianthera and Geodorum densiflorum are orchids having secondary metabolites that are thought to have antioxidant activity. This study aims to determine the antioxidant activity of the ethanolic extracts of these two types of orchids using the DPPH method (1,1-diphenyl-2-picrylhydrazine). The results showed that the IC50 of the ethanolic extract of the leaves of D. lasianthera was 288.24 ppm and the stem was 26.27 ppm. The IC50 of the ethanol extract of leaves of G. densiflorum was 277.65 ppm and of the tubers was 197.48 ppm. Finally, the leaves have negligible antioxidant activity, but the stems and tubers had activity ranging from weak to very strong.

Download Full-text

Phytochemicals Screening and Antioxidant Effectiveness of Garlic (Allium sativum) from Timor Island

Biosaintifika Journal of Biology & Biology Education ◽

10.15294/biosaintifika.v11i1.17313 ◽

2019 ◽

Vol 11 (1) ◽

pp. 1-7 ◽

Cited By ~ 1

Author(s):

Melania Priska ◽

Natalia Peni ◽

Ludovicus Carvallo

Keyword(s):

Free Radicals ◽

Free Radical ◽

Secondary Metabolites ◽

Allium Sativum ◽

Positive Impact ◽

Ethanolic Extract ◽

The Body ◽

Natural Medicine ◽

The People ◽

Dpph Method

The people of Timor Island only know garlic as a kitchen spice. This research provides new knowledge of the benefits of garlic in the health sector, especially as an inhibitor of free radical that can trigger various degenerative diseases. The aims of this research were to identify secondary metabolites contained in the ethanolic extract of garlic (Allium sativum L.) from Timor Island and to determine its effectiveness in inhibiting free radicals. The method used to test secondary metabolites was phytochemical screening using color reagents. Testing the effectiveness of free radical inhibitors from garlic ethanol extract from Timor Island was carried out in 2 stages: 1.) Determination of DPPH maximum wavelength (λ) and 2.) Measurement of antioxidant activity using DPPH method. The results showed that the ethanolic extract of garlic from Timor Island contained secondary metabolites of flavonoids, phenols and terpenoids. It was also very effective in inhibiting free radicals, with the acquisition of IC50 values <50 ppm which was equal to 9.729 ppm. This research gives some information that can be used for the pharmacological ingredients, i.e. as a natural medicine that safe for the body to be consumed by the people of Timor Island. Moreover that also can impact on demand of garlic in the market. This has a very positive impact on improving the economy of garlic farmers on Timor Island.

Download Full-text

Photostability Study on Character and Antioxidant Activity of Tomato Extract (Solanum lycopersicum l.) in Nanostructured Lipid Carrier (NLC) and Conventional Creame

International Journal of Drug Delivery Technology ◽

10.25258/ijddt.v7i1.8919 ◽

2017 ◽

Vol 7 (1) ◽

Cited By ~ 1

Author(s):

Noorma Rosita ◽

Dewi Haryadi ◽

Tristiana Erawati ◽

Rossa Nanda ◽

Widji Soeratri

Keyword(s):

Antioxidant Activity ◽

Physical Stability ◽

Nanostructured Lipid Carrier ◽

Uvb Radiation ◽

K Value ◽

Antioxidant Power ◽

Solanum Lycopersicum L ◽

Tomato Extract ◽

Dpph Method ◽

High Shear Homogenization

The aim of this study was to investigate the ability of NLC in increasing photostability of tomato extract in term of antioxidant activity. Photostability testing on antioxidant activity of samples were conducted by accelerating method using UVB radiation 32.400 joule for 21 hours radiation. Antioxidant activity was measured by DPPH method. NLC was made by High Shear Homogenization (HPH) method at 24000 rpm for 4 cycles, while conventional creame was made by low speed at 400 rpm. The product were characterized include: pH, viscosity, and particle size. There were had difference characters and physical stability. NLC had smaller size, more homogenous and more stable than conventional creame. It was known that stability of antioxidant activity of tomato extract in NLC system higher than in conventional creame. That was showed with k value, as constanta of rate scavenging activity decreasing in antioxidant power between time (Sigma 2-tail less than 0.005) of NLC and conventional creame were: 2.03x10-2 %/hour ±0.08 (3.94) and 4.71x 10-2 %/ hour ±0.23 (4.88) respectively.

Download Full-text

Évaluation de la teneur des composés phénoliques, des proprieties antioxydantes et antimicrobiennes de l’espèce Erica arborea L. (Ericaceae) dans la médecine traditionnelle du Tell sétifien dans l’Est Algérien

Phytothérapie ◽

10.3166/phyto-2019-0210 ◽

2019 ◽

Author(s):

K. Yaici ◽

S. Dahamna ◽

I. Moualek ◽

H. Belhadj ◽

K. Houali

Keyword(s):

Activité Antioxydante ◽

Activité Antimicrobienne ◽

Médecine Traditionnelle ◽

Composés Phénoliques ◽

Bacillus Subtilus ◽

Erica Arborea ◽

Infections Urinaires ◽

Flavonoïdes Totaux ◽

Extraits Aqueux ◽

Β Carotene

Les sommités florales d’Erica arboea L. sont largement utilisées sous forme d’infusion en médecine traditionnelle et sont recommandées dans le Tell sétifien pour traiter et prévenir les infections urinaires aiguës et chroniques principalement. Nous avons soumis les extraits aqueux des feuilles et des fleurs à un dosage des polyphénols, des flavonoïdes et à la détermination des activités antioxydante et antimicrobienne. Les teneurs en composés phénoliques et flavonoïdes totaux ont montré une corrélation avec les activités antioxydantes évaluées. Les tests antioxydants utilisés (DPPH, FRAP, CAT, blanchiment du β-carotène et piégeage du radical hydroxyle) ont montré que le test du DPPH a donné la meilleure activité radicalaire. En outre, l’extrait des feuilles s’est mieux exprimé dans les tests du DPPH, du FRAP et du β- carotène. Les tests de la CAT et le radical hydroxyle ont par contre révélé une activité antioxydante plus importante pour l’extrait de fleurs. Pour l’activité antimicrobienne, on a utilisé la méthode de diffusion en milieu gélosé en ayant recours à la méthode des puits. Les deux extraits ont montré une activité antimicrobienne contre les bactéries à Gram positif Staphylococcus aureus ATCC25923, Bacillus subtilus CLAM20302, Bacillus cereus CLAMH300. L’extrait des feuilles a été plus actif avec une valeur variant de 15 à 23 mm comparé à celui des fleurs (17–18,5 mm). Les valeurs de la concentration minimale inhibitrice ont été trouvées dans une gamme variant de 6,25 à 25 mg/ml pour les extraits des feuilles et des fleurs respectivement. Les résultats de l’étude peuvent enrichir les données existantes et montrent que les feuilles et les fleurs d’Erica arborea L. constituent une source d’agents antioxydants et antibactériens.

Download Full-text

Comparative Evaluation of Chemical Composition, Phenolic Compounds, and Antioxidant and Antimicrobial Activities of Tropical Black Bolete Mushroom Using Different Preservation Methods

Foods ◽

10.3390/foods10040781 ◽

2021 ◽

Vol 10 (4) ◽

pp. 781

Author(s):

Jaturong Kumla ◽

Nakarin Suwannarach ◽

Keerati Tanruean ◽

Saisamorn Lumyong

Keyword(s):

Chemical Composition ◽

Phenolic Compounds ◽

Natural Antioxidants ◽

Antimicrobial Activities ◽

Fresh Sample ◽

Antioxidant Power ◽

Preservation Methods ◽

Ferric Reducing Antioxidant Power ◽

Nutritional Benefits ◽

Antioxidant And Antimicrobial Activities

Tropical black bolete, Phlebopus portentosus, provides various nutritional benefits and natural antioxidants to humans. In this study, the chemical composition, phenolic compounds, and antioxidant and antimicrobial activities of fresh mushroom samples and samples stored for a period of one year using different preservation methods (drying, brining, and frozen) were investigated. The results indicated that the brining method significantly reduced the protein and fat contents of the mushrooms. The polyphenol and flavonoid contents of the frozen sample were not significantly different from that of the fresh sample. The results revealed that an inhibition value of 50% (IC50) for the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay of the extract of the dried and frozen samples was not statistically different from that of the fresh sample. The IC50 value of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay and ferric reducing antioxidant power (FRAP) value in the extract of the frozen sample were not found to be significantly different from those of the fresh sample. Furthermore, the lowest degree of antioxidant activity was found in the extract of the brined sample. Additionally, the antimicrobial activities of the extracts of the fresh and frozen samples were not significantly different and both extracts could have inhibited the growth of all tested Gram-positive bacteria and Pseudomonas aeruginosa.

Download Full-text

FRACTIONATION OF THE N-HEXANE EXTRACT OF GARCINIA BANCANA MIQ. (MANGGIS HUTAN) LEAVES AND ITS ANTIOXIDANT ACTIVITY BASED ON 1,1-DIPHENYL-2- PICRYLHYDRAZYL AND FERRIC REDUCING ANTIOXIDANT POWER ASSAYS

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2018.v10s1.90 ◽

2018 ◽

Vol 10 (1) ◽

pp. 407

Author(s):

Dewi Kumala Putri ◽

Berna Elya ◽

Nuraini Puspitasari

Keyword(s):

Antioxidant Activity ◽

Antioxidant Activities ◽

Hexane Extract ◽

Phytochemical Screening ◽

Active Fraction ◽

Leaf Extracts ◽

Antioxidant Power ◽

Dpph Method ◽

Ferric Reducing Antioxidant Power

Objective: To assess the antioxidant activity from another part of the plant, in this study, leaf extracts in n-hexane were fractionated.Methods: Ten fractions were obtained and tested in vitro for antioxidant activity using two methods, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferricreducing antioxidant power (FRAP), to identify the most active fraction.Results: The IC50 of the most active fraction was 36.24 μg/mL using the DPPH method, and the EC50 was 39.54 μg/mL using the FRAP method. Themost active fraction was also shown to contain terpenoids.Conclusion: The most active fraction of an n-hexane extract of the leaves of Gacinia bancana Miq., which was tested by both DPPH and FRAP methodshad antioxidant activities with IC50 and EC50 values of 36.2482 μg/mL and 39.5442 μg/mL, respectively. Phytochemical screening showed that activefraction contains terpenoids.

Download Full-text

Chemical Analysis and Evaluation of Antioxidant, Antimicrobial, and Photoprotective Activities of Schinopsis brasiliensis Engl. (Anacardiaceae)

The Scientific World JOURNAL ◽

10.1155/2017/1713921 ◽

2017 ◽

Vol 2017 ◽

pp. 1-10 ◽

Cited By ~ 3

Author(s):

Sarah Raquel Gomes de Lima-Saraiva ◽

Fernanda Granja da Silva Oliveira ◽

Raimundo Gonçalves de Oliveira Junior ◽

Camila de Souza Araújo ◽

Ana Paula de Oliveira ◽

...

Keyword(s):

High Performance ◽

Ethanolic Extract ◽

Antimicrobial Activities ◽

Physicochemical Analysis ◽

Minimal Bactericidal Concentration ◽

Native Plant ◽

Protection Factor ◽

Dpph Method ◽

High Concentrations

Schinopsis brasiliensis Engl. is a native plant of Caatinga which has high concentrations of compounds capable of absorbing ultraviolet light, suggesting its potential application for the development of sunscreen preparations. After its identification and collection, this vegetable drug was submitted to a physicochemical analysis through the preparation of ethanolic extract. The phytochemical screening and analysis of extracts were carried out by high-performance liquid chromatography (HPLC) evaluation. The antioxidant activity of the extract was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) method and β-carotene bleaching test. Inhibitory hemolytic activity and morphological deformation of erythrocytes induced by H2O2 were also demonstrated and the antimicrobial activity was analyzed by the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) method. For the in vitro determination of the sun protection factor (SPF), the spectrophotometric method was used. From the analyses carried out with this species, this plant showed significant results for the antioxidant and antimicrobial activities, as well as sunscreen action. Important flavonoids were identified. These data are an important step for the development of new photoprotective cosmetic with Caatinga species, revealing importance and representing another incentive for the preservation of the species involved and analyzed in the study.

Download Full-text

NEW FLAVONOIDS FROM THE AERIAL PARTS OF POLYGONUM EQUISETIFORME SM (POLYGONACEAE)

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i2.15593 ◽

2017 ◽

Vol 9 (2) ◽

pp. 166 ◽

Cited By ~ 2

Author(s):

Sayed A. El-toumy ◽

Joslin Y. Salib ◽

Nabila H. Shafik ◽

Asmaa S. Abd Elkarim ◽

Gihan A. Mick

Keyword(s):

Antioxidant Activity ◽

Methyl Ester ◽

Secondary Metabolites ◽

Gallic Acid ◽

Total Phenolic ◽

Flavonoid Content ◽

Isolation And Identification ◽

Aerial Parts ◽

Dpph Method ◽

Phenolic And Flavonoid

Objective: The current study was to deal the isolation and identification of secondary metabolites from Polygonum equisetiforme and evaluation of antioxidant activity of its extract.Methods: The methanol-water extract (7:3) of the air-dried aerial parts of Polygonum equisetiforme was fractionated and separated to obtain the isolated compounds by different chromatographic techniques. Structures of these compounds were elucidated by UV and 1D⁄2D H⁄ C NMR spectroscopy and compared with the literature data. The crude extract was evaluated for in vitro antioxidant activity using the 2,2 diphenyl dipicryl hydrazine (DPPH) method.Results: Ten secondary metabolites were isolated from Polygonum equisetiforme in this study. Of which three new flavonoids named as 3,5,7,2’,5’ pentahydroxyflavone 3-O-b-D-glucopyranoside (1), 3,5,7,2’,5’ pentahydroxyflavone 3-O-b-D-glucopyranoside 8 C-sulphated (2) and quercetin 3-O-β-D-glucucorinde 6''-methyl ester 8-sulphated (3) as well as quercetin 3-O-β-D-glucucorinde methyl ester (4), quercetin 3-O-β-D-glucopyranoside (5), quercetin 7-O-β-D-glucopyranoside (6), quercetin(7), myricetin (8), P-methoxy gallic acid methyl ester (9) and gallic acid (10). The antioxidant potential of P. equisetiforme extract was evaluated by investigating it's total phenolic and flavonoid content and DPPH radical scavenging activity whereby the extract showed significant antioxidant activity (IC50 = 37.45 μg/ml). The total phenolic and flavonoid content was found to be 130.79±5.502 and 45.8±1.63 μg/ml, respectively.Conclusion: Polygonum equisetiforme is a promising medicinal plant, and our study tends to support the therapeutic value of this plant as an antioxidant drug.

Download Full-text