scholarly journals PENGARUH PEMBERIAN EKSTRAK BIJI PEPAYA (CARICA PAPAYA L.) TERHADAP KUALITAS SPERMATOZOA TIKUS WISTAR (RATTUS NORVEGICUS)

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Rionaldy Walansendow ◽  
Janette M. Rumbajan ◽  
Lydia Tendean

Abstract: Participation of men on using contraceptive is still low. Carica papaya L. contains alkaloid chemicals which act as antifertility agent and can be used as contraceptives for men. This study was carried out to find the effects of Carica papaya L. seed extracts toward the spermatozoa qualities. This study was conducted to nine male wistar rats (Rattus norvegicus) weighing from 200 to 250 grams, aging from 12 to 15 months. These nine healthy adult rats were divided into 3 groups of 3 rats each. 50 and 70 mg/kg/day of the extract were orally administrated to group 1 and 2 respectively, while group 3 were treated as a negative control group. The daily administration was carried out for 50 days, after which the animals of group 1, 2 and 3 were sacrificed. This study found a decreased quality of spermatozoa (i.e morphology) in group 1 and 2 (p = 0,000). Thus, confirms it’s contraceptive efficacy on men’s fertility.Keywords: carica papaya l. seed, spermatozoaAbstract: Partisipasi pria dalam menggunakan alat kontrasepsi masih kurang. Tanaman pepaya mengandung senyawa alkaloid yang bersifat antifertilitas dan dapat digunakan sebagai bahan untuk kontrasepsi pria. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh dari ekstrak biji pepaya (Carica papaya L.) terhadap kualitas spermatozoa. Penelitian eksperimental ini dilakukan pada sembilan ekor tikus wistar jantan (Rattus norvegicus) dengan berat badan 200-250 gram berumur 12-15 bulan. Sembilan ekor wistar dibagi menjadi tiga kelompok, dan satu kelompok terdiri dari tiga ekor tikus wistar. Selama 50 hari, ekstrak biji pepaya (Carica papaya L.) diberikan pada kelompok perlakuan 1 sebesar 50 mg/kgBB per hari, sedangkan pada kelompok perlakuan 2 sebesar 70 mg/kgBB per hari. Kelompok kontrol tidak diberikan perlakuan. Setelah 50 hari hewan coba pada kelompok kontrol, perlakuan 1, dan perlakuan 2 diterminasi. Hasil penelitian menunjukkan terjadi penurunan kualitas spermatozoa berupa morfologi spermatozoa pada tikus wistar yang diberikan ekstrak biji papaya (Carica papaya L.) (p = 0,000). Hasil tersebut menunjukkan bahwa ekstrak biji pepaya mempunyai efek antifertilitas terhadap fertilitas pria.Kata kunci: biji pepaya, spermatozoa

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Bill C. A. Bogar ◽  
Lydia Tendean ◽  
Grace L. A. Turalaki

Abstract: Infertility is an inability of couples to reproduce after carrying out sexual intercourses for at least a year without using any contraceptives. There are 10-15% couples worldwide who experience infertility problems and almost one half of them is on men. Spermatozoa qualities including concentration, motility, and morphology are used as an indicator for men’s fertility. The utilization of herbal medicine (i.e. Eurycoma longifolia Jack extracts) is now being an alternative way to improve fertility among the community. This study was carried out to find the effects of Eurycoma longifolia Jack on spermatozoa qualities. This experimental study was conducted to nine male wistar rats (Rattus norvegicus) weighing from 200-250 grams, aging from 12-15 months. These nine adult rats were divided into 3 groups of 3 rats each. 400 and 600 mg/kg/day of Eurycoma longifolia Jack extracts were orally administrated to group 1 and 2 respectively, while group 3 were treated as control group. After 50 days, the animals of group 1, 2 and 3 were sacrificed. As a result, there is an improvement in qualities of spermatozoa which are statistically significant (p<0,05) in group 2. Thus, provide the beneficial effects of 600mg/kg Eurycoma longifolia Jack extracts on the qualities of spermatozoa.Keywords: pasak bumi, spermatozoaAbstrak: Infertilitas adalah ketidakmampuan pasangan untuk dapat hamil setelah satu tahun berhubungan intim tanpa menggunakan kontrasepsi. Ada sekitar 10 – 15 % pasangan mengalami masalah infertilitas dan hampir setengahnya masalah ada pada pria. Kualitas spermatozoa meliputi konsentrasi, motilitas dan morfologi spermatozoa merupakan salah satu indikator fertilitas pada pria. Penggunaan tanaman herbal atau yang lebih dikenal jamu telah menjadi pengobatan alternatif di masyarakat. Tanaman herbal yang dimaksud adalah pasak bumi. Penelitian ini bertujuan untuk mengetahui efek dari esktrak pasak bumi (Eurycoma longifolia Jack) terhadap kualitas spermatozoa. Penelitian ini menggunakan 9 ekor tikus wistar jantan (Rattus norvegicus) dengan berat badan 200-250 g berumur 12-15 bulan. Sembilan ekor wistar dibagi menjadi tiga kelompok, dan satu kelompok terdiri dari tiga ekor tikus wistar. Penelitian ini dilaksankan selama 50 hari. Esktrak pasak bumi (Eurycoma longifolia Jack) diberikan dengan dosis 400mg/kgBB per hari pada perlakuan 1 dan dosis 600 mg/kgBB per hari pada perlakuan 2. Setelah 50 hari hewan coba pada kelompok kontrol, perlakuan 1 dan perlakuan 2 diterminasi. Hasil penelitian memperlihatkan terjadi peningkatan kualitas spermatozoa yang signifikan secara statistik (p<0,05) pada perlakuan 2 terhadap kelompok kontrol. Hasil tersebut menunjukan bahwa Esktrak pasak bumi (Eurycoma longifolia Jack) pada dosis 600 mg/kgBB dapat meningkatkan kualitas spermatozoa.Kata kunci: pasak bumi, spermatozoa


2019 ◽  
Vol 12 (02) ◽  
Author(s):  
Husnul Khatimah ◽  
Saifuddin Sirajuddin ◽  
Zainal Zainal

ABSTRACT In diabetics there is usually an increase in free fatty acids in the blood and increases the concentration of cholecerols and triglycerides, this condition is called diabetes dyshidemia. This study aimed to find out at the effect of bran milk treatment on the changes of the concetration of cholesterol and triglycerides in diabetetic white wistar rats. Conducted on May 15 until 15 August 2018. Experimental research pre-post test with control design. Subjects of twenty wistar white rats were made diabetic by injecting alloxan 140mg / kg. After being declared diabetic, the rats were divided into four groups, namely group 1 (negative control), group 2 (positive control), group 3 (bran milk 0.54g / 200 gr) and group 4 (bran milk 1.08g / 200gr). The intervention was given for fourteen days. The data analysis used the paired T-test to test the subject before and afterthe treatment and the One-Way ANOVA test was used to test the difference between the groups. The research results indicated that  the treatment with bran milk there were no significant differences in cholesterol and triglyceride concentrations in the four groups. But when viewed from changes before and after the intervention showed that group 4 (bran milk 1.08g / 200gr) decreased cholesterol concentration and in group 3 (bran milk 0.54g / 200gr) a decrease in triglycerides was better than group 1 (control negative) and group 2 (positive control). It was concluded that the administration of bran milk can reduce the concentration of cholesterol and triglycerides in wistar diabetic white rats.Keywords : Bran milk, Cholesterol, Triglycerides, Rats, Diabetic


Author(s):  
D. Muhammed ◽  
E. O. Dada ◽  
F. O. Iyaji ◽  
O. J. Abraham ◽  
N. A. Chijioke

The leaf, stem and bark of Eucalyptus citriodora are combined for use in the treatment of malaria in Anyigba, North Central, Nigeria. However, no scientific investigations have been carried out to know the effect of this plant on biochemical parameters of experimental mice. Thus, this study evaluated the biochemical parameters of mice infected with Plasmodium berghei after the administration of ethanolic leaf extract of E citriodora. Twenty-four (24) mice of body weights between 18-25 g were grouped into six groups. Group 1, infected but not treated (negative control), group 2, infected and administered with 0.2 mg/kg of chloroquine (positive control), group 3, not infected, but administered with 0.2 ml of normal saline (normal control), while the remaining three (3) groups were infected and treated with 200, 400 and 800 mg/kg body weight of the extract respectfully. The pack cell volume (PCV) was assessed before and after infection and treatment using standard procedure. The mice were sacrificed on the sixth day and blood samples were collected for biochemical analysis using standard methods. The PCV in mice of all groups decrease significantly (p<0.05), except group 3 (normal control) that increased. The alanine transaminase (ALT), aspartate transaminase (AST), total bilirubin level was higher in negative control (group 1) than in all other groups studied, but it was higher in the group treated with 200 mg/kg bd wt of the extract than in the positive control and the groups treated with 400 and 800 mg/kg body weight of the extract. A similar trend was observed in the levels of cholesterol, triglycerides, high density lipolipid (HDL) and low density lipolipid (LDL). The level of creatinine, blood urea and nitrogen were observed to be low in groups 1 and 2, compared to other groups. This study revealed that E. citriodora ethanolic leaf extract does not exact toxic effect on the internal organs like liver, kidney and heart.


2015 ◽  
Vol 12 (6) ◽  
pp. 25-33
Author(s):  
A A Babakhin ◽  
A A Laskin ◽  
O Yu Kamishnikov ◽  
N N Shershakova ◽  
I P Shilovskiy ◽  
...  

The purpose of this study was to develop a mouse model of asthma (MMA) using house dust mite Dermatophagoides pteronyssinus (Der p) extract. Methods. BALB/c mice were i.p. immunized with different doses of Der p lyophilized extract three times in three week interval in the mixture with Al(OH)3. 8 weeks after the final immunization mice were challenged with Der p during five consecutive days by intranasal applications (INA) or aerosol administration (AA). All mice were divided into 5 experimental groups: group 1 was immunized with 50 ^g/mouse of Der p (in protein equivalent) in the mixture with 2 mg/mouse Al(OH)3 and challenged by INA; group 2 was immunized in the same way and challenged by AA; group 3 was immunized with 100 ^g/mouse Der p in the mixture with 2 mg/mouse of Al(OH)3 and challenged by INA; group was immunized in the same way and challenged by AA; group 5 (negative control) was immunized and challenged with saline. 24 hours after the last challenge airway hyperresponsiveness (AHR) to different concentrations of metha-choline was evaluated in all groups by whole-body plethysmography. 48 hours after the last challenge in all groups blood was collected for differential cell count, brochoalveolar lavage fluid (BALF) was sampled for the determination of inflammatory cells and lungs were removed for histological analysis. Histopathological changes in lungs (allergic inflammation) were graded according to semi-quantitative scoring system. Anti-Der p IgE, IgG1 and IgG2a antibodies in individual sera samples were detected by ELISA seven days after the last immunization and 48 hours after the challenge. Results. The levels of anti-Der p IgE antibodies in groups 1-4 before as well as after the challenge were substantially higher than that of in the group 5 (negative control). The highest level of serum Der p-specific IgE antibodies was observed in the group 2. The levels of anti-Der p IgG1 and IgG2a antibodies in the groups 1-4 during all periods of observation were higher than that of group 5 (negative control). At the same time the maximal levels of anti-Der p IgG1 and IgG2a antibodies were observed in group 3 both after immunization and after challenge. The maximum of AHR was observed in the groups 1 and 3 challenged by INA. Analysis of cell composition in BALF demonstrated significant elevated number of eosinophils in group 3 in comparison with group 5 (negative control) and other experimental groups. Regarding peripheral blood leukocyte count we observed decreasing of band neutrophils in group 4 and increasing of segmented neutrophils in groups 1 and 3 in compare to group 5 (negative control). In group 1 we found statistically significant decreasing of lymphocytes and increasing of eosinophils in compare to negative control group 5. Histological picture of general allergic inflammation in lungs as well as peribronchial and perivascular infiltration with inflammatory cells were the most noticeable (according to score system) in group 3 in comparison with negative control group and other experimental groups. Conclusion. Data obtained indicate that immunization (sensitization) of mice with Der p in a dose 100 μg/mouse together with Al(OH)3 and challenge with Der p by mean of intranasal applications is a suitable approach for modeling of mouse allergic asthma.


2014 ◽  
Vol 2 (1) ◽  
Author(s):  
Kallan A. B. Pulio ◽  
Christi Mambo

Abstract: Background: Pluchea indica Less. is one of the widely known and widely distributed native potential medicinal plants in Indonesia. Objectives: To determine the antipyretic effect of pluchea indica Less. abstract on male Wistar rats (Rattus norvegicus). Metods: This is an experimental study conducted on 15 male Wistar rats with weight ranging from 150-200 gram. Study animals were divided into 5 groups, namely positive control group, negative control group and three intervention group to whom pluchea indica extract in various doses were administered. Temperature measurement was performed before the administration of DPT-HB vaccine, 2 hours after administration of the vaccine, as well as pn the 30th, 60th, 90th, and 120th minute after intervention. Result: There was a slight reduction in mean rectal temperature of Wistar rats administered with Pluchea indica Less. extract. Conclusion: Pluchea indica Less. extract has no antipyretic effect on rats. Keywords: Pluchea indica Less., Antipyretic effect, DPT-HB vaccine, Rat  Abstrak: Latar belakang: Salah satu tumbuhan asli Indonesia yang dikenal dan tersebar dengan luas pada beberapa daerah serta berpotensi untuk dikembangkan menjadi tanaman obat yaitu tanaman Beluntas (Pluchea indica (L.) Less.). Tujuan: Penelitian ini bertujuan untuk mengetahui ada tidaknya efek antipiretik ekstrak daun beluntas (Pluchea indica (L.) Less.) pada tikus Wistar (Rattus norvegicus) jantan. Metode: Penelitian ini menggunakan metode eksperimental. Hewan coba yang digunakan adalah tikus wistar jantan sebanyak 15 ekor dengan berat badan 150-200 gram. Hewan coba dibagi 5 kelompok yaitu kelompok kontrol negatif (aquades), kelompok kontrol positif (parasetamol), dan kelompok perlakuan yaitu pemberian ekstrak daun Beluntas. Pengukuran suhu dilakukan sebelum pemberian vaksin DPT-HB, 2 jam setelah pemberian DPT-HB, dan menit ke-30, 60, 90, 120 setelah pemberian perlakuan. Hasil: Suhu rata-rata rectal tikus yang diberikan ekstrak daun beluntas mengalami penurunan sedikit. Kesimpulan: Ekstrak daun beluntas tidak mempunyai efek antipiretik pada tikus. Kata Kunci : daun beluntas (Pluchea indica Less.), efek antipiretik, vaksin DPT-HB, tikus.


2019 ◽  
Vol 20 (1) ◽  
pp. 12-18
Author(s):  
Sameh El-Nabtity

The present study aimed to investigate the prophylactic effect of Cymbopogon proximus and Alhagi maurorum on Sulfadimidine induced urolithiasis in rabbits . Thirty New Zealand male rabbits were allocated into six equal groups (each of five): Group (1) was used as a negative control. Group(2) were administered sulfadimidine (200mg/kg) by intramuscular injection.Groups(3) and (4) were administered sulfadimidine(200mg/kg) by intramuscular injection and 330mg/kg of Cymbopogon proximus alcoholic and aqueous extracts respectively orally.Groups(5) and (6) were administered sulfadimidine(200mg/kg) by intramuscular injection and 400mg/kg of Alhagi maurorum alcoholic and aqueous extracts respectively orally. The period of experiment was 10 days. Blood and urine samples were collected from rabbits on the 10th day. The results recorded a significant decrease in serum creatinine, urea, uric acid and crystalluria in Cymbopogon proximus and Alhagi maurorum groups compared to sulfadimidine treated group.We conclude that Cymbopogon proximus and Alhagi maurorum have a nephroprotective and antiurolithiatic effects against sulfadimidine induced crystalluria.


Author(s):  
Nur Fitri

Background: Peperomia pellucida L'HBK or known as messengers in the Indonesian plant is a herbaceous plant that belongs to the family Piperaceae. This study aimed to determine the effect of the stem and leaf extract cream messengers to the healing process of burns in rats (Rattus norvegicus. Methods: This was an experimental study using a completely randomized design. Test animals were divided into three groups, each - each group consisted of 3 rats. The first group is the negative control group (distilled water), the second group is a positive control group (Bioplacenton®), the third group is the group treated stem and leaf extract cream errand. The diameter of the wound and fibroblasts observed histopathology and is used as an indicator of the healing process of burns. The burns were treated and observed the healing effect for 20 days. Data were analyzed statistically wound diameter using ANOVA followed by LSD test. Results: The results showed the cream extracts of stems and leaves telling effect on the healing process of burns on rats. Conclusion: The results also showed that the treatment group and the leaf stem extract cream messengers and control groups positively influence the healing process of burns significantly when compared to the negative control group. Meanwhile, the treatment group stem and leaf extract cream messengers have no preformance difference influence the healing process of burns a significant positive control group


Animals ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 519 ◽  
Author(s):  
Joanna Kuźniacka ◽  
Marcin Hejdysz ◽  
Mirosław Banaszak ◽  
Jakub Biesek ◽  
Sebastian Kaczmarek ◽  
...  

The aim of the study was to analyze the quality of geese meat receiving feed with soybean meal (group 1), yellow lupin (group 2), narrow-leaved lupin (group 3), or white lupin (group 4). In total, 400 male White Kołuda® geese were randomly assigned to four groups, with 10 replicates and 10 birds each, during the 77-day rearing period. After the end of the rearing period, 10 birds from each group were slaughtered and dissected. Meat quality traits were measured. Based on the production results, it can be concluded that geese use fodder with yellow and white lupin to the same degree as in the case of the control group and higher body weight gain was recorded in the first rearing period. In contrast, the use of narrow-leaved lupin in mixtures for geese worsened the feed used. Meat traits were similar in all groups, including the content of muscles and fat in the carcass (p > 0.05), excluding abdominal fat. The weight of abdominal fat and its proportion in the carcass were higher (p < 0.05) in geese from group 4. A higher (p < 0.05) pH was found in group 1. The protein and intramuscular fat content in breast muscles was highest (p < 0.05) in geese from group 4, and a higher water content was found in group 1. The protein content in leg muscles was higher in group 3, and the fat content was higher in group 4 (p < 0.05). The color and water-holding capacity of meat were comparable in all groups (p > 0.05). The analysis revealed a positive effect of replacing soybean meal with alternative protein sources, especially yellow and white lupin, on the growth performance and quality of goose meat.


2018 ◽  
Vol 12 (3) ◽  
pp. 196-200
Author(s):  
Ajita Rathi ◽  
Prateeksha Chowdhry ◽  
Mamta Kaushik ◽  
Pallavi Reddy ◽  
Roshni Roshni ◽  
...  

Background. The present study was undertaken to evaluate the incidence of dentinal cracks during root canal preparation with different periodontal ligament simulating materials in vitro. Methods. Seventy freshly extracted human mandibular first premolars were selected and divided into 7 groups in terms of simulating material: group 1: polyether impression material; group 2: polyvinyl acetate adhesive; group 3: polyvinyl siloxane impression material; group 4: cyanoacrylate adhesive; group 5: epoxy resin adhesive; group 6: positive control, without any periodontal ligament simulation; and group 7: negative control, where neither a periodontal ligament simulating material was used nor canal preparation was carried out. Root canal preparation was carried out in all the groups followed by sectioning of roots at 3 mm, 6 mm and 9 mm. The sections were evaluated under a stereomicroscope at ×2.5 for the presence or absence of cracks. Chi-squared test was used to compare the appearance of defective roots between the different experimental groups. Results. The least number of cracks were found in the negative control group, followed by group 1 where polyether impression material was used for periodontal ligament simulation. The difference was significant with a P-value of 0.002 for coronal sections. Conclusion. Under the limitation of the present study, polyether and polyvinyl siloxane (light body) can both be used for simulation of periodontal ligament.


2018 ◽  
Vol 2 ◽  
pp. 39
Author(s):  
Shahinur Nahar Moury ◽  
Md. Touhiduzzaman Sarker ◽  
Adhita Sri Prabakusuma ◽  
Md. Imrul Hasan Russel ◽  
Md. Shahidul Islam

The main purpose of this study was to explore the effects of Spirulina (Spirulina platensis) as a feed supplement by replacing Vitamin-Mineral Premix on the performance of broiler. The study explored that final body weight was 1039, 1070, 1044, 1065, 1117 and 893 g/bird in group 1, 2, 3, 4, 5 and 6 where significantly (p<0.5) higher weight observed in Spirulina group (5) but lower in negative control group (6). Feed intake observed more or less similar in all groups (1483±24g; p>0.5). Feed conversion ratio (kg FI/kg LWG) observed significantly different like 1.72, 1.65, 1.69, 1.62, 1.61 and 1.99 in group 1, 2, 3, 4, 5 and 6 respectively (p<0.5) where higher in negative group (6) and control group (1), but is lowest and similar in 75% Spirulina group (4) and highest (100%) Spirulina group (5). %Ash of different dietary groups were 46.35, 46.54, 48.93, 49.93, 55.07 and 46.92 of group 1, 2, 3, 4, 5 and 6 respectively. The %ash of bone was gradually increased among dietary groups and the best result was obtained in 100% Spirulina group 5 (55.07). It also revealed that diets containing no vitamin-mineral premix with 100% Spirulina improve the performance of broiler. The findings suggest that poultry farmer can use spirulina as a feed item for broiler for more yield.


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