scholarly journals UJI DAYA HAMBAT ORGANISME LAUT SPONS Amphimedon sp., TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus, Escherichia coli, DAN Jamur Candida albicans

PHARMACON ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 561
Author(s):  
Rizky Akbar Latif ◽  
Defny S. Wewengkang ◽  
Henki Rotinsulu
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Inhibitory Activity ◽  
Marine Animals ◽  
Anti Cancer ◽  
Anti Inflammatory ◽  
Anti Fungal ◽  
The City

Sponges are marine animals that can produce bioactives that are useful as anti-virus, anti-fungal, antibiotic, anti-cancer, anti-inflammatory, and antioxidants. Amphimedon sp., sponge itself is found in the waters of Lembeh islands, the City of Bitung. This study was to determine the inhibitory activity against the microorganisms growth of Amphimedon sp., sponge, against microbes of Staphylococcus aureus, Escherichia coli, and Candida albicans. Based on the results of the study, the extracts and fractions of Amphimedon sp., sponge samples did not have microbial inhibitory activity against the test microbes of Staphylococcus aureus, Escherichia coli, and Candida albicans Keywords : Amphimedon sp., Antimicrobial, Staphylococcus aureus, Escherichia coli, Candida albicans   ABSTRAK Spons merupakan hewan laut yang dapat menghasilkan bioaktif yang bermanfaat sebagai anti virus, anti jamur, antibiotik, anti kanker, anti inflamasi, dan antioksidan. Spons Amphimedon sp sendiri ditemukan didaerah perairan pulau Lembeh kota bitung. Penelitian ini yaitu untuk mengetahui aktivitas daya hambat pertumbuhan mikroorganisme dari spons Amphimedon sp., terhadap mikroba Staphylococcus aureus, Escherichia coli, dan Candida albicans. Berdasarkan hasil penelitian menunjukkan ekstrak dan fraksi sampel spons Amphimedon sp., tidak memiliki aktivitas daya hambat terhadap mikroba uji Staphylococcus aureus, Escherichia coli, dan Candida albicans Kata kunci : Amphimedon sp., Antimikroba, Staphylococcus aureus, Escherichia coli, Candida albicans

scholarly journals UJI AKTIVITAS ANTIMIKROBA EKSTRAK DAN FRAKSI SPONS Liosina paradoxa DARI PERAIRAN DESA TUMBAK MINAHASA TENGGARA TERHADAP PERTUMBUHAN MIKROBA Staphylococcus aureus, Escherichia coli, DAN Candida albicans

PHARMACON ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 629
Author(s):  
Walen E. Oeiyano ◽  
Herny E. I. Simbala ◽  
Henki Rotinsulu
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Diffusion Method ◽  
Chloroform Fraction ◽  
Growth Inhibitors ◽  
Marine Animals ◽  
Average Value ◽  
Ethanol Extracts

ABSTRACT Sponges are marine animals, which contain active compounds known to have potential in the pharmaceutical field, including the antimicrobials. The purpose of this study was to determine the activity of microorganism growth inhibitors from the sponge Liosina paradoxa against microbes Staphylococcus aureus, Escherichia coli, and Candida albicans. Samples were extracted by maceration method with ethanol solvent and fractionation using methanol, chloroform, and n-hexane solvents. Antimicrobial activity was carried out by agar diffusion method (Kirby and Bauer). Methanol fractions of sponge Liosina paradoxa showed the greatest antimicrobial activity against Escherichia coli with an average value of 11.33 mm, and categorize as strong, chloroform fraction was 7.33 mm and categorize as medium, and n-hexane fraction was 7.16 mm and categorize as medium, ethanol extracts was 7.83 mm and categorize as moderate antimicrobial activity. Keywords: Liosina paradoxa, Antimicrobial, Staphylococcus aureus, Escherichia coli, Candida      albicans. ABSTRAKSpons merupakan merupakan hewan laut yang mengandung senyawa aktif yang diketahui berpotensi dalam bidang farmasi, diantaranya sebagai antimikroba. Tujuan dari penelitian ini yaitu untuk mengetahui aktivitas penghambat pertumbuhan mikroorganisme dari spons Liosina paradoxa terhadap mikroba Staphylococcus aureus, Escherichia coli, dan Candida albicans.Sampel diekstraksi dengan metode maserasi dengan pelarut etanol dan fraksinasi menggunakan pelarut metanol, kloroform, dan n-heksan. Aktivitas antimikroba dilakukan dengan  metode difusi agar (Kirby and Bauer). Fraksi metanol spons Liosina paradoxa menunjukkan aktivitas antimikroba paling besar terhadap Escherichia coli dengan nilai rata-rata 11,33 mm, dengan kategori kuat, fraksi kloroform 7,33 mm kategori sedang, fraksi n-hexan 7,16 mm kategori sedang, ekstrak etanol 7,83 mm kategori aktivitas antimikroba sedang. Kata kunci: Liosina paradoxa, antimikroba, Staphylococcus aureus ,Escherichia coli, Candida       albicans.

scholarly journals AKTIVITAS ANTIMIKROBA DARI EKSTRAK DAN FRAKSI KARANG LUNAK Sarcophyton sp., DARI PERAIRAN PONTENG DESA TUMBAK MINAHASA TENGGARA TERHADAP MIKROBA PATOGEN Staphylococcus aureus, Escherichia coli, dan Candida albicans

PHARMACON ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 991
Author(s):  
Triswa A. Mokodongan ◽  
Herny E. I. Simbala ◽  
Henki Rotinsulu
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Natural Product ◽  
Microbial Growth ◽  
Soft Coral ◽  
Chemical Compounds ◽  
Natural Medicine ◽  
Escherichia Coli Bacteria ◽  
Anti Fungal

ABSTRACTSoft coral sarcophyton sp., poruduces natural chemical compounds and is known as a natural product. These natural chemical compounds have the potential as a source of natural medicine. Soft coral sarcophyton sp., has antimicrobial, antibacterial, anti-fungal, anti-tumor,neurotoxic, and anti-inflammatory activities that are beneficialt to the pharmaceutical industry. This study aims to determine the biological  activity of soft corals Sarcophyton sp., collected from the waters of Ponteng Tumbak Village, Southeast Minahasa Regency on microbial growth of staphylococcus aureus, Escherichia coli, and Candida albicans. Soft coral samples were macerated with 96% of ethanol solvent and partitioned liquid-liquid using methanol, chloroform, and n-hexane solvents. Staphylococcus aureus and Escherichia coli bacteria with a medium category,  and were unable to inhibit the growth of the fungus Candida albicans.Keywords: Sarcophyton sp., Antimicrobial, Staphylococcus aureus, Escherichia coli, Candida albicans.ABSTRAKKarang lunak Sarcophyton sp., merupakan salah satu jenis karang lunak yang memproduksi senyawa kimia alami dan dikenal dengan istilah natural product. Senyawa kimia alami tersebut berpotensi sebagai sumber obat alami. Karang lunak Sarcophyton sp., mempunyai aktivitas antimikroba, antibakteri, anti fungi, anti tumor, neurotoksik, dan anti inflamasi yang bermanfaat bagi industry farmasi.  Karang lunak Sarcophyton sp., ditemukan di daerah perairan Poteng Desa Tumbak Minahasa Tenggara. Penelitian ini bertujuan untuk mengetahui adanya aktivitas daya hambat pertumbuhan mikroorganisme dari Karang lunak Sarcophyton sp., terhadap mikroba Staphylococcus aureus, Escherichia coli, dan Candida albicans. Sampel diekstraksi metode maserasi dengan pelarut etanol dan fraksinasi menggunakan pelarut methanol, kloroform, dan n-heksan. Berdasarkan hasil penelitian menunjukkan bahwa masing-masing ekstrak dan fraksi  mampu menghambat pertumbuhan bakteri Staphylococcus aureus dan Escherichia coli dengan kategori daya hambat sedang. Sedangkan masing-masing ekstrak dan fraksi pada Jamur Candida albicans tidak mampu menghasilkan aktivitas antibakteri. Kata Kunci :  Sarcophyton sp., Antimikroba, Staphylococcus aureus, Escherichia coli, dan Candida albican.

Screening and antimicrobial activity of actinomycetes isolated from the rhizosphere of Clitoria ternatea

2016 ◽  
Vol 1 (01) ◽  
Author(s):  
Vemavarapu Bhaskara Rao ◽  
Kandlagunta Guru Prasad ◽  
Krishna Naragani ◽  
Vijayalakshmi Muvva
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Calcium Carbonate ◽  
Rhizosphere Soil ◽  
Soil Samples ◽  
Antimicrobial Spectrum ◽  
Clitoria Ternatea ◽  
Primary Screening

The air dried rhizosphere soil samples pretreated with calcium carbonate was employed for the isolation of actinomycete strains. Serial dilution plate technique was used for the isolation of actinomycetes. A total of 20 actinomycete strains designated as BS1-BS20 were isolated from the rhizosphere of medicinal plant Clitoria ternatea. All the 20 strains were subjected to primary screening for antimicrobial activity. Among the 20 strains screened, 10 strains exhibited high antimicrobial spectrum against Staphylococcus aureus, Escherichia coli and Candida albicans.

The In vitro Evaluation of the Antimicrobial Activity of Quercus robur L. Methanolic and Aqueous Leaves’ Extracts, from the Algerian High Plateaus Against some Uropathogenic Microbial Strains

2019 ◽  
Vol 18 (5) ◽  
pp. 262-274
Author(s):  
E. Benyagoub ◽  
N. Nabbou ◽  
S. Boukhalkhel ◽  
I. Dehini
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Enterococcus Faecalis ◽  
Fusidic Acid ◽  
Quercus Robur ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Microbial Strains

The medicinal value of the plants is due to their chemical components that bring a definite physiological action on the human body to prevent the diseases. In this work, we investigated the antimicrobial activity of leaves’ extracts of Quercus robur L., collected from the Algerian upper highlands, on ten bacterial strains and one fungal strain known to be pathogenic. First, we performed a qualitative phytochemical analysis, and second, antimicrobial activity tests performed by agar diffusion method (disc and well) with the determination of MIC by broth macro-dilution method. Given the results, it appears that obtained macerates of Quercus robur L. were rich in bioactive phytoconstituents such as alkaloids, anthraquinones, saponins, tannins, and other components. The yield of aqueous and methanolic macerates of leaves was 8.5 ± 1.41 and 22.4 ± 4.36%, respectively. The bacterial resistance was relatively important to several antibiotics, namely, ampicillin, amoxicillin + clavulanic acid for strains of Escherichia coli and Salmonella sp. However, Staphylococcus aureus strains were resistant to fusidic acid, penicillin, and oxacillin; while Enterococcus faecalis was resistant to fusidic acid, penicillin, oxacillin, and ticarcillin. The antibacterial activity of the macerates toward tested microbial strains showed that the aqueous and methanolic macerates of the leaves were proportional to the tested concentration and active not only against Gram-positive and Gram-negative bacteria but also on the fungal species Candida albicans. The estimated MIC for Escherichia coli, Enterococcus faecalis, and Staphylococcus aureus was in the order of 10 mg/mL, which seems more effective than toward Salmonella sp., Klebsiella pneumoniae, Pseudomonas aeruginosa, and Candida albicans which were in the order of 30 mg/mL. These preliminary results confirm that the part of the studied plant had a very good antimicrobial activity that was proportional to the serial concentrations of the tested extracts.

Substituted 4-Formyl-2H-chromen-2-ones: Their Reaction with N-(2,3,4,6-Tetra-Oacetyl- β-D-galactopyranosyl)thiosemicarbazide, Antibacterial and Antifungal Activity of Their Thiosemicarbazone Products

2020 ◽  
Vol 24 (19) ◽  
pp. 2272-2282
Author(s):  
Vu Ngoc Toan ◽  
Nguyen Minh Tri ◽  
Nguyen Dinh Thanh
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Selenium Dioxide ◽  
Antibacterial And Antifungal Activity ◽  
E Coli ◽  
Antibacterial And Antifungal Activities ◽  
Alkyl Ethers ◽  
Antibacterial And Antifungal

Several 6- and 7-alkoxy-2-oxo-2H-chromene-4-carbaldehydes were prepared from corresponding alkyl ethers of 6- and 7-hydroxy-4-methyl-2-oxo-2H-chromen-2-ones by oxidation using selenium dioxide. 6- and 7-Alkoxy-4-methyl-2H-chromenes were obtained with yields of 57-85%. Corresponding 4-carbaldehyde derivatives were prepared with yields of 41-67%. Thiosemicarbazones of these aldehydes with D-galactose moiety were synthesized by reaction of these aldehydes with N-(2,3,4,6-tetra-O-acetyl-β-Dgalactopyranosyl) thiosemicarbazide with yields of 62-74%. These thiosemicarbazones were screened for their antibacterial and antifungal activities in vitro against bacteria, such as Staphylococcus aureus, Escherichia coli, and fungi, such as Aspergillus niger, Candida albicans. Several compounds exhibited strong inhibitory activity with MIC values of 0.78- 1.56 μM, including 8a (against S. aureus, E. coli, and C. albicans), 8d (against E. coli and A. niger), 9a (against S. aureus), and 9c (against S. aureus and C. albicans).

Influence of Removable Denture Cleaning Agents on Adhesion of Oral Pathogenic Microflora In Vitro: A Randomized Controlled Trial

2021 ◽  
Vol 14 (1) ◽  
pp. 656-664
Author(s):  
I.R. Volchkova ◽  
A.V. Yumashev ◽  
V.V. Borisov ◽  
V.I. Doroshina ◽  
E.A. Kristal ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Test Group ◽  
Control Group ◽  
Removable Dental Prostheses ◽  
Removable Denture ◽  
Group 2 ◽  
Adhesion Index ◽  
Group 1

Introduction: Removable dentures are used by 20% of the population. These may be accompanied by denture stomatitis in 15-70% of patients. The choice of the optimal cleansing agent for removable dental prostheses is of high significance. Aim: The aim of our research was to study the influence of removable denture cleansing products on the adhesion of microorganisms and yeast. Materials and Methods: We manufactured 144 specimens of standardized round shape with a diameter of 10 mm from 4 types of modern polymeric materials used by prosthetic dentistry to produce removable dentures, 12 specimens of each material were placed into suspensions of bacterial cultures of Staphylococcus aureus, Escherichia coli, Candida albicans, then into “ClearaSept” (Test group 1), “Рrotefix active cleanser” (Test group 2), saline solution (Control group), followed by nutrient media. The adhesion index was calculated and analyzed. Results: There was no reliable lowering of adhesion index of Staphylococcus Aureus to all materials detected in Test group 1 (U=6, p>0.05 for Bio XS; U=8, p>0.05 for Dental D, Denotokeep Peek, Vertex Rapid Simplified). In Test group 2, the adhesion index of Staphylococcus Aureus reliably decreased to all materials compared to the Control group (U=0, p≤0.01). The adhesion index of Candida albicans and Escherichia coli to all materials in Test group 1 had a minor to moderate reliable reduction compared to the Control group (U=0, p≤0.01). Test group 2 showed a significant reliable decrease in Candida albicans and Escherichia coli adhesion index to all materials in comparison with the Control group (U=0, p≤0.01). Conclusion: The research showed an unreliable or minor and moderate reliable decrease in microorganisms adhesion index depending on the microorganism species after treatment of denture material specimens by antibacterial soap “ClearaSept” and a reliable significant decrease in microbial and yeast adhesion after application of Protefix active cleaner solution, which demonstrates a more significant antimicrobial effect in comparison to “ClearaSept” against Staphylococcus aureus, Escherichia coli, and Candida albicans.

scholarly journals A Novel Antimicrobial Phenanthrene Alkaloid from Bryopyllum pinnatum

2011 ◽  
Vol 8 (3) ◽  
pp. 1456-1461 ◽  
Author(s):  
Donatus Ebere Okwu ◽  
Fred Uchenna Nnamdi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Aspergillus Niger ◽  
Ethanolic Extract ◽  
Klebsiella Pneumonia ◽  
Antimicrobial Studies ◽  
Prevention And Treatment ◽  
Kalanchoe Pinnata ◽  
Isolated Compound

Phenenthrene alkaloid identified as 1-ethanamino 7 hex-1-yne-5I-one phenanthrene was isolated from the ethanolic extract of the leaves ofBryophyllum pinnatum(syn. B. calcinum kalanchoe pinnata) a versatile Nigeria medicinal plant. The structure was elucidated using NMR, IR, UV and MS spectral data. Antimicrobial studies showed that the isolated compound successfully inhibitedPsuedomonas aeruginosa, Klebsiella pneumonia, Staphylococcus aureus, Escherichia coli, Candida albicansandAspergillus niger. This result authenticates the use ofbryophyllum pinantumin phytomedicine for disease prevention and treatment of infections.

scholarly journals Antimicrobial Effect of Plasma-Activated Tap Water on Staphylococcus aureus, Escherichia coli, and Candida albicans

Water ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 1480
Author(s):  
William Chiappim ◽  
Aline da Graça Sampaio ◽  
Felipe Miranda ◽  
Mariana Fraga ◽  
Gilberto Petraconi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Tap Water ◽  
Antimicrobial Effect ◽  
Gliding Arc ◽  
Oxidation Reduction ◽  
Treatment Conditions ◽  
Oxidation Reduction Potential

In this study, the potential antimicrobial activity of plasma-activated tap water (PAW) was evaluated against Staphylococcus aureus, Escherichia coli, and Candida albicans. For this, PAW was prepared in a gliding arc plasma system using two treatment conditions: stagnant water and water stirring by a magnetic stirrer, called moving water. Subsequently, their oxidation-reduction potential (ORP), pH, electrical conductivity (σ), and total dissolved solids (TDS) were monitored in different areas of the sample divided according to the depth of the beaker. It was observed that PAW obtained in dynamic conditions showed a more uniform acidity among the evaluated areas with pH 3.53 and ORP of 215 mV. Finally, standardized suspensions of Staphylococcus aureus (ATCC 6538), Escherichia coli (ATCC 10799), and Candida albicans (SC 5314) were treated with PAW, and the reduction of viable cells determined the antimicrobial effect. Our results indicate that the tap water, activated by plasma treatment using gliding arc, is an excellent inactivation agent in the case of Staphylococcus aureus and Escherichia coli. On the other hand, no significant antimicrobial activity was achieved for Candida albicans.

A REVIEW OF PHYTOMORPHOLOGICAL, PHYTOCHEMICAL AND PHARMACOLOGICAL ACTIVITY ON IPOMOEA CARNEA

2021 ◽  
Vol 12 (4) ◽  
pp. 166-169
Author(s):  
Mahaveer Sing ◽  
Sravan P Kumar ◽  
Birendra Shrivastava ◽  
Pamula B Reddy ◽  
Suma Rohilla
Keyword(s):  
Wound Healing ◽  
Pharmacological Activity ◽  
Wild Plant ◽  
Toxicological Effects ◽  
Ipomoea Carnea ◽  
Anti Cancer ◽  
Anti Inflammatory ◽  
Anti Fungal

Ipomoea carnea Jacq. grows as wild plant in India. It is identified as a useful material for several applications including medicinal purposes. Different extracts of Ipomoea carnea plant possess anti-bacterial, anti-fungal, antioxidant, antimicrobial, anti-cancer, anti-convulsant, immune modulatory, antidiabetic, hepatoprotective, anti-inflammatory, anxiolytic, sedative, cardiovascular, inhibition and wound healing activities. However, some toxicological effects have been also reported. In this review the potential of phytochemical, pharmacological and other activities of Ipomoea carnea are discussed.

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