A study on the cytotoxic activity of marine sponges on the embryo development of sea urchin Diadema savigny

2013 ◽  
pp. 107
Author(s):  
Esther D Angkouw ◽  
Remy E. P Mangindaan
Keyword(s):  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Sea Urchin ◽  
High Probability ◽  
Ethanolic Extract ◽  
Marine Sponges ◽  
Cytotoxic Assay ◽  
Cytotoxic Compound ◽  
Axinella Corrugata ◽  
Cytotoxic Compounds

A study on the cytotoxic activity of five sponges has been conducted. The samples were collected from Malalayang beach, extraced with ethanol to give ethanolic extract. The ethanolic extratc then partitioned with hexane, ethyl acetate, buthanol to yield three fractions. Crude extracts, hexane and chloroform fractions were subjected to cytotoxic assay using fertilized Sea urchin eggs. The results showed that sponge Theonalla sp. contained a potential cytotoxic compound(s). After partitioned lay open that  dissolve fraction ethyl acetate showed activity of highest cytotoxic. The study gives a high probability in discovering many novel cytotoxic compounds from the sea© Suatu penelitian tentang pengujian aktivitas sitotoksik dari lima jenis spons (Petrosia nigricans, Plakinolophia mirabilis, Axinella corrugata, Ianthella basta, Theonella sp.) telah dilakukan. Spons yang diambil dari perairan Malalayang diekstrak dengan etanol. Ekstrak etanolik dipartisi dengan  pelarut heksan, etil asetat dan butanol. Ekstrak etanolik dan fraksi-fraksi diujikan pada sel telur bulu babi Diadema savigny yang telah dibuahi. Pengamatan dilakukan selama 48 jam. Hasil pengujian menunjukkan bahwa kontrol mencapai tahap pluteus sedangkan pengujian ekstrak etanolik dari kelima jenis spons diperoleh hasil Theonella sp. mempunyai aktivitas tertinggi. Sampel spons Theonella sp. mampu menghambat/memperlambat pembelahan sel bulu babi sampai pengamatan 48 jam hanya sampai pada pembelahan 4. Dari pengujian ketiga fraksi menunjukkan  bahwa fraksi etil asetat memiliki aktivitas sitotoksik yang tinggi dibanding fraksi heksan dan fraksi butanol. Hasil penelitian ini membuka peluang besar penemuan berbagai senyawa aktif sitotoksik dari organisme laut khususnya spons yang berasal dari perairan Sulawesi Utara©

scholarly journals Profil Fraksi Sitotoksik terhadap Sel Murine Leukemia P-388 dari Ekstrak Biji Honje (Etlingera elatior)

2017 ◽  
Vol 3 (1) ◽  
pp. 79-87
Author(s):  
Alfindah Rusanti ◽  
Dede Sukandar ◽  
Tarso Rudiana ◽  
Adawiah Adawiah
Keyword(s):  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Mtt Assay ◽  
Chemical Structure ◽  
Ethyl Acetate Extract ◽  
Artemia Salina ◽  
Cytotoxic Compounds ◽  
Murine Leukemia ◽  
Etlingera Elatior

The research characterization of cytotoxic fraction against P-388 leukemia murine cells from the extract honje (Etlingera elatior) seed have been reported. This research lead to isolated and characterization of cytotoxic compounds against P-388 leukemia murine cells from the extract E. elantior seed. The extract of E. elantior seed was maserated by methanol, n-hexane, and ethyl acetate, respectively and estimated their cytotoxic activity against P-388 leukemia murine cell with 3- (4, 5-dimetiltiazol-2-yl) -2,5-difeniltetrazolium bromide (MTT) assay guided toxicity test against of shrimp Artemia salina Leach. Brine shirmp Lethality Test (BSLT) method. The active extracts will be separated by fractionation using column chromatography, radial chromatography, and for analyzing the purity of isolate will estimate by HPLC. The chemical structure of pure isolate will be identified by spectroscopies data UV Vis, FTIR, NMR and MS. The ethyl acetate extract from honje seed have cytotoxic activity by leukemia P-388 cell  with IC50 19.21 µg/mL. The compound toxic as cytotoxicagainst P-388 leukemia murine cells is flavonoid compouds their is resveratrol, lapachol, apigenin, methylated chrysin, 6,2’-dihydroxyflavanone, 3-hydroxy-3,4’-dymethoxyflavone and 4’-hydroxy-5,7-dimethoxyflavanone.DOI: http://dx.doi.org/10.15408/jkv.v0i0.3640

scholarly journals Cytotoxic Activity of Six Samples of Brazilian Propolis on Sea Urchin (Lytechinus variegatus) Eggs

2013 ◽  
Vol 2013 ◽  
pp. 1-4 ◽  
Author(s):  
C. C. Fernandes-Silva ◽  
J. C. Freitas ◽  
A. Salatino ◽  
M. L. F. Salatino
Keyword(s):  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
High Activity ◽  
Sea Urchin ◽  
The State ◽  
Lytechinus Variegatus ◽  
Cytotoxic Activities ◽  
Highly Active ◽  
Artepillin C ◽  
Brazilian Propolis

The cytotoxic activities of extracts of four samples of propolis from the state of Minas Gerais (Southeast Brazil) and two from the state of Paraná (South Brazil) were evaluated using sea urchin (Lytechinus variegatus) eggs. Cytotoxic activity was observed, characterized mainly by the inhibition of the first cleavage of newly fertilized eggs. Methanol extracts at 32 µg mL−1of all samples were highly active (97–100%). Extracts were also prepared by successive treatments of the samples with hexane, chloroform, ethyl acetate, and methanol. High activity was observed using the ethyl acetate fractions of all samples, but hexane and chloroform fractions of some samples also had high activity. Based on the chemical composition of the extracts and fractions (published previously), it is hypothesized that the cytotoxic activities observed are due mainly to artepillin C,p-coumaric acid, and kaempferide. The results suggest that caffeoylquinic acids have no cytotoxic activity in sea urchin eggs.

scholarly journals Cytotoxic Activities of (Graptophyllum pictum (L.) Griff) Ethanolic Extract and Its Fractions on Human Colon Cancer Cell WiDr

2020 ◽  
Vol 25 (1) ◽  
pp. 29
Author(s):  
Azizah Amin ◽  
Andayana Puspitasari Gani ◽  
Retno Murwanti
Keyword(s):  
Colon Cancer ◽  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Ethanolic Extract ◽  
Human Colon ◽  
Cytotoxic Activities ◽  
Human Colon Cancer ◽  
Water Fraction ◽  
Cancerous Cell ◽  
Ic50 Values

Colon cancer is the third most common cancer in the world. Treatments for colon cancer might cause major side effects, hence increase opportunities for the development of new cancer drugs. One of the plants that potential to develop as an anticancer agent is daun ungu (Graptophyllum pictum (L.) Griff). From previous studies, G.pictum had cytotoxic activity against several cancerous cell lines. Traditionally, G.pictum leaves have been used for hemorrhoid treatment. The purpose of this study was to determine the cytotoxic activity of G.pictum ethanolic extract and its fractions on human colon cancer WiDr cells and to elucidate the compounds contained in most active extracts/fractions. G.pictum was extracted using 70% ethanol and fractionated using n-hexane, chloroform, and ethyl acetate, respectively. The yield of the extract obtained was 18.9%. The yield of hexane, chloroform, ethyl acetate, and ethanol-water fractions were 1.07%, 2.93%, 10.26%, and 84.82%, respectively. The cytotoxic activity was carried out on WiDr cells using the MTT assay. Cytotoxic activity was determined based on IC50 values. IC50 value of extract, hexane, chloroform, ethyl acetate and ethanol-water fraction obtained on WiDr cells were 1527,58; 143,97; 507,19; 3538,67 and 3186,60 μg/mL. The hexane fraction containing terpenoids and phenolics showed the highest cytotoxic activities against WiDr colon cancer cells.

Synthesis and Cytotoxicity Evaluation of Novel Indole Derivatives as Potential Anti-Cancer Agents

2019 ◽  
Vol 15 (8) ◽  
pp. 873-882
Author(s):  
Mona M. Kamel ◽  
Mohamed K. Abdel-hameid ◽  
Hala B. El-Nassan ◽  
Eman A. El-Khouly
Keyword(s):  
Cytotoxic Activity ◽  
Indole Alkaloids ◽  
Marine Sponges ◽  
Cytotoxic Agents ◽  
Wide Range ◽  
Anti Cancer ◽  
Effective Inhibition ◽  
Cytotoxic Compounds ◽  
Hct 116

Background: Marine sponges and tunicates have been a wealthy source of cytotoxic compounds such as indole alkaloids. Most of the indole alkaloids show in vitro cytotoxic and antineoplastic activities against a wide range of cancer cell lines. Objective: Three series of bioisosteres of marine indole alkaloids (meridianins) were synthesized and the compounds were tested for their in vitro anti-proliferative activity against HCT-116 cellline. In the design of the targeted analogues, the 2-aminopyrimidine ring of merdianins was replaced with 5-aminopyrazole, pyrazolo[1,5-a]pyrimidine and pyrazolo[3,4-b]pyridine rings. Results: The cytotoxic screening of the synthesized compounds revealed that pyrazolo[1,5- a]pyrimidines (compounds 9c and 11a) had the most potent cytotoxic activity with IC50 = 0.31 μM and 0.34 μM respectively. Compounds 9c and 11a were further investigated for their kinase inhibitory potencies toward six kinases (CDK5/p25, CK1ð/ε, GSK-3α/β, Dyrk1A, Erk2, and CLK1). They exhibited effective inhibition of GSK-3α/β (IC50 = 0.196 μM and 0.246 μM, respectively) and Erk2 (IC50 = 0.295 μM and 0.376 μM, respectively). Conclusion: Meridianins emerged as promising lead structures that need further development to obtain more selective and potent cytotoxic agents. One of these modifications involved the replacement of 2-aminopyrimidinyl ring of meridianins with other heterocyclic rings. Both pyrazolo[ 1,5-a]pyrimidine and pyrazolo[3,4-b]pyridine rings showed promising cytotoxic activity compared to the five membered 5-aminopyrazole.

scholarly journals Efek Sitotoksik Ekstrak dan Fraksi Umbi Paku Atai Merah (Angiopteris ferox Copel) Terhadap Sel Kanker Payudara T47D

2020 ◽  
Vol 6 (2) ◽  
Author(s):  
Andi Nur Aisyah ◽  
Syamsu Nur ◽  
Endang Lukitaningsih ◽  
Rumiyati Rumiyati ◽  
Asril Burhan ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Cancer Cells ◽  
Supportive Therapy ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Screening Tests

The use of natural products has been widely used as a resource of new bioactive chemical compounds. One of them is the Paku Atai Merah (Angiopteris ferox Copel) tuber which has long been used empirically by the Dayak tribe of East Kalimantan as an anti-cancer. The purpose of this study was to determine the anticancer cytotoxic activity of the extract and fractions of Paku Atai Merah tuber against T47D breast cancer cells in vitro. Extract of Paku Atai Merah tubers was obtained by maceration method using ethanol solvent until obtained the ethanolic extract then fractionated using various solvents to obtain n-hexane, ethyl acetate, and aqueous-ethanol fractions. The cytotoxic effect was carried out based on the MTT assay. Phytochemical screening tests showed positive results for the presence of flavonoid, phenolic, tannin, saponin and steroid compounds. The results of the cytotoxic activity study showed that the ethyl acetate fraction had moderate cytotoxic activity in T47D cancer cells with an IC50 value  of 84.8 µg/ml. Ethanol extract (513.06 µg/ml) and n-Hexane frsction (881.97 µg/ ml) were also included in the weak category. This study indicates that ethyl acetate fraction can be developed as a supportive therapy for breast cancer treatment.  

scholarly journals Aktivitas Sitotoksik Ekstrak Etanol, Fraksi Etanol-Air, Etil Asetat serta N-Heksana Buah Pare (Momordica charantia) pada Sel MCF-7 secara In-Vitro

2019 ◽  
Vol 16 (2) ◽  
pp. 73-82
Author(s):  
Adhe Retnantya Pamungkas ◽  
Peni Indrayudha
Keyword(s):  
Breast Cancer ◽  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Ethanolic Extract ◽  
Ethyl Acetate Fraction ◽  
Hexane Fraction ◽  
Assay Method ◽  
Water Fraction ◽  
Mcf 7

Bitter melon is one of fruit that have pharmacological effects such as an anticancer which potentially active on breast cancer cell MCF-7. The aim of this study was to compare the potential cytotoxic activity between ethanol extract of pare and the results of ethanol, ethyl acetate and n-hexane fraction on MCF-7 breast cancer cells and to determine the class of compounds contained in each sample. Pare’s powder was extracted with maceration method in 80% ethanol solvent and then continue to fractionated in 96% ethanol, ethyl acetate and hexane solvent. Extract and fractions were continue to cytotoxic assay using the MTT assay method. Cytotoxic test results showed that the ethanolic extract had no potential cytotoxic activity. Ethyl acetate fraction with the highest concentration 16 µg/mL has the highest potential inhibition 43,87% on MCF-7 cells population. Extract and fractions than continue to phytochemical screening with Thin Layer Chromatography (TLC) method. TLC detection showed that n-hexane fraction contained more compound groups than ethanolic extract, ethanolic-water fraction and ethyl acetate fraction.

scholarly journals EFEK PEMACUAN APOPTOSIS FRAKSI ETIL ASETAT EKSTRAK ETANOL AKAR PASAK BUMI (Eurycoma longifolia Jack) TERHADAP SEL HeLa

2012 ◽  
Vol 2 (1) ◽  
Author(s):  
Isnaniati Romadhoni Indarwati Nurkhasan
Keyword(s):  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Hela Cells ◽  
Ethanolic Extract ◽  
Ethyl Acetate Fraction ◽  
Test Method ◽  
Chloroform Fraction ◽  
Eurycoma Longifolia ◽  
Apoptosis Assay ◽  
Eurycoma Longifolia Jack

Pasak bumi is one of the Indonesian original plants having anticancer activity. Eurycomanone was isolated from Eurycoma longofolia, Jack  root was proven to have cytotoxic effect and induce apoptosis on Hela cells. The objective this study was to know the cytotoxic activity of ethyl acetate fraction of ethanolic extract of the Eurycoma longifolia, Jack root on Hela cells and the mode of death. Ethyl acetate fraction was obtained from  the ethyl acetate soluble fraction from residue of chloroform fraction of ethanolic extract of Eurycoma longifolia Jack roots. The cytotoxic assay was performed using MTT test method with concentration series used in this study were 1000; 750; 500; 400; 200; 100; 50; 12,5; 6,25; 3,125; and 1,5625 µg/ml. Absorbance was measured at wavelength of 550 nm using ELISA reader. Apoptosis assay were observed using Giemsa staining. MTT assay indicated that etil asetat fraction of ethanolic extract of the Eurycoma longofolia, Jack root showed cytotoxic activity againts Hela cells with IC50 value 3,70 µg/ml. The result of apoptosis assay indicated that the ethyl acetate fraction of ethanolic extract of the Eurycoma longofolia, Jack root induce apoptosis. The apoptotic cells was observed wrinkle, rounded and dark colors leading to chromatin condensation.

Cytotoxic Constituents from the Bark of Erythrina poeppigiana Against the MCF-7 Breast Cancer Cell Lines

2020 ◽  
Vol 10 (3) ◽  
pp. 257-261
Author(s):  
Tati Herlina ◽  
Merlin ◽  
Mohd. Azlan ◽  
Unang Supratman
Keyword(s):  
Breast Cancer ◽  
Cytotoxic Activity ◽  
Chemical Structure ◽  
Breast Cancer Cell Lines ◽  
Erythrina Poeppigiana ◽  
Structure Activity ◽  
Cancer Line ◽  
Cytotoxic Compounds ◽  
Ic50 Values ◽  
Mcf 7

Background: Erythrina poeppigiana (Leguminosae) is a high-growing plant with an orange flower that is widely distributed in tropical and subtropical countries. This particular plant is widely used in traditional medicine for gynecological complications and the treatment of various diseases. There exists no previous information regarding cytotoxic compounds from this plant. Objective: This research is to isolate cytotoxic compounds from E. poeppigiana. Methods: The isolation step was carried out using a combination of chromatographic techniques to obtain isolated three compounds (1, 2, and 3). Results: The chemical structure of isolated compounds was elucidated by spectroscopic methods and identified as β-erythroidine (1), 8-oxo-β-erythroidine (2), and 8-oxo-α-erythroidine (3). Compounds (1-3) showed cytotoxic activity against MCF-7 breast cancer line with IC50 values of 36.8, 60.8 and 875.4 μM, respectively. Conclusion: Three compounds have been successfully isolated from Erythrina poeppigiana (Leguminosae), showing cytotoxic properties against MCF-7 breast cancer line. Structure-activity relationship studies showed that the presence of enone moiety on compound 1 can reduce its cytotoxic activity towards MCF-7 breast cancer line.

scholarly journals In Vitro Cytotoxic Effects of Secondary Metabolites Present in Sarcopoterium Spinosum L.

2021 ◽  
Vol 11 (11) ◽  
pp. 5300
Author(s):  
Jozef Hudec ◽  
Jan Mojzis ◽  
Marta Habanova ◽  
Jorge A. Saraiva ◽  
Pavel Hradil ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Benzalkonium Chloride ◽  
Ethanol Extract ◽  
Cytotoxic Activities ◽  
Sarcopoterium Spinosum ◽  
Mcf 7

Sarcopoterium spinosum (L.) is a medicinal plant traditionally used for the treatment of various diseases including cancer in the Near- and Middle East. The fractions and constituents of the ethanol extract of S. spinosum were screened for in vitro cytotoxic activities on Jurkat (acute T-lymphoblastic leukemia), HeLa (cervical adenocarcinoma), MCF-7 (mammary gland adenocarcinoma), Caco-2 (human colorectal adenocarcinoma), and MDA-MB-231 (mammary gland adenocarcinoma) cell lines using the MTT (3-(dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The ethanol extract was subsequently re-extracted with ethyl acetate and in its sub-fraction obtained by column chromatography three compounds (stachydrine, benzalkonium chloride and rutine) were the first time identified by nuclear magnetic resonance (NMR) analyses. The most active subfraction showed cytotoxic activity against HeLa, MCF-7, and Caco-2 cell lines. The three compounds mentioned, as standards of high-performance liquid chromatography (HPLC) quality, were studied individually and in combination. Cytotoxic activity observed might be due to the presence of benzalkonium chloride and rutin. Benzalkonium chloride showed the strongest growth suppression effect against HeLa cells (IC50 8.10−7 M) and MCF-7 cells (IC50 5.10−6 M). The mixture of stachydrine and benzalkonium chloride allowed a synergistic cytotoxic effect against all tested cancer and normal cells to be obtained. Anti-cancer activity of the plant extract of S. spinosum remains under-investigated, so this research describes how the three major compounds identified in the ethyl acetate extract can exert a significant dose dependent in vitro cytotoxicity.

scholarly journals Potential of Thai Herbal Extracts on Lung Cancer Treatment by Inducing Apoptosis and Synergizing Chemotherapy

Molecules ◽  
2020 ◽  
Vol 25 (1) ◽  
pp. 231 ◽  
Author(s):  
Juthathip Poofery ◽  
Patompong Khaw-on ◽  
Subhawat Subhawa ◽  
Bungorn Sripanidkulchai ◽  
Apichat Tantraworasin ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Ethyl Acetate ◽  
Cancer Treatment ◽  
Apoptotic Cell ◽  
Primary Lung Cancer ◽  
A549 Cells ◽  
Cancer Cell Line ◽  
Ethanolic Extract ◽  
Lung Cancer Cell Line ◽  
Lung Cancer Treatment

The incidence of lung cancer has increased while the mortality rate has continued to remain high. Effective treatment of this disease is the key to survival. Therefore, this study is a necessity in continuing research into new effective treatments. In this study we determined the effects of three different Thai herbs on lung cancer. Bridelia ovata, Croton oblongifolius, and Erythrophleum succirubrum were extracted by ethyl acetate and 50% ethanol. The cytotoxicity was tested with A549 lung cancer cell line. We found four effective extracts that exhibited toxic effects on A549 cells. These extracts included ethyl acetate extracts of B. ovata (BEA), C. oblongifolius (CEA), and E. succirubrum (EEA), and an ethanolic extract of E. succirubrum (EE). Moreover, these effective extracts were tested in combination with chemotherapeutic drugs. An effective synergism of these treatments was found specifically through a combination of BEA with methotrexate, EE with methotrexate, and EE with etoposide. Apoptotic cell death was induced in A549 cells by these effective extracts via the mitochondria-mediated pathway. Additionally, we established primary lung cancer and normal epithelial cells from lung tissue of lung cancer patients. The cytotoxicity results showed that EE had significant potential to be used for lung cancer treatment. In conclusion, the four effective extracts possessed anticancer effects on lung cancer. The most effective extract was found to be E. succirubrum (EE).

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