scholarly journals PENENTUAN KANDUNGAN PIGMEN KAROTENOID PADA KEPITING Grapsus albolineatus (Lamarck) BETINA DARI PERAIRAN PESISIR PANTAI DESA TANAWANGKO

2017 ◽  
Vol 5 (3) ◽  
pp. 75
Author(s):  
Sutandi Makalalag ◽  
Darus saadah Paransa ◽  
Desy M H Mantri
Keyword(s):  
Pigment Content ◽  
Mature Stage ◽  
Carotenoid Pigment ◽  
Epidermal Layer ◽  
Maturation Process ◽  
Gonadal Maturation ◽  
Total Pigment ◽  
Pigment Type ◽  
Layer Chromatography ◽  
Β Carotene

Grapsus albolineatus (Lamarck) is one of the species of blackish-green crab found above or below the coastal rocks. At has long legs and no swimming legs and has a small claw. Purple capitals, is characteristic of this type of crab., the G. albolineatus crab has an attractive color on the carapace organ that indicates the presence of pigment content. This study was aimed to determined the content and it’s pigment type of the organs of the carapace, epidermal layer, hepatopancreas, blood and gonads in the female G. albolineatus (Lamarck) crab. The method of this research in order to separated and determinated of pigment content by using thin layer chromatography (TLC) method. The results obtained in this study were the total pigment content of G. albolineatus crab showed the highest value in gonad organ with value 34,41 μg, followed by epidermal layer organ 12,19 μg, hepatopancreas 9,61 μg, blood 1,06 μg and carapace 0.42 μg. the pigment content of the gonads organ has the highest value compared with other organs, it is presumed that the female G. albolineatus crab is at the mature stage of the gonad, so that the carotenoid pigment is still accumulated on the gonad organ used for the gonadal maturation process. The types of pigment identified in the extract of the carapace organ, epidermal layer, hepatopancreas, gonads and blood from female G. albolineatus crabs with semipolar solution of Petroleum Eter and Acetone are: β-carotene, echinone, kantaksantin, adonirubin type, astaxanthine and astacene .Keywords: Grapsus albolineatus, TLC, pigmentGrapsus albolineatus (Lamarck) merupakan salah satu spesies kepiting yang berwarna hitam kehijauan yang ditemukan di atas atau di bawah batu pantai. Memiliki kaki jalan yang panjang dan tidak memiliki kaki renang serta memiliki capit yang berukuran kecil. Capit berwarna ungu, merupakan ciri khas kepiting jenis ini. Kepiting G. albolineatus memiliki warna yang menarik pada organ karapas yang mengindikasikan adanya kandungan pigmen. Penelitian ini bertujuan untuk menentukan kandungan dan jenis pigmen pada organ karapas, lapisan epidermis, hepatopankreas, darah dan gonad pada kepiting G. albolineatus (Lamarck) betina. Pemisahan yang umum digunakan dalam penentuan jenis pigmen karotenoid adalah menggunakan metode Kromatografi Lapis Tipis. Pemisahan ini dikenal karena proses pemisahannya mudah, sederhana dan membutuhkan waktu yang relatif singkat serta dapat menghasilkan data yang akurat. Hasil yang diperoleh dalam penelitian ini adalah kandungan pigmen total dari kepiting G. albolineatus menunjukkan nilai tertinggi pada organ gonad dengan nilai 34,41 g, diikuti organ lapisan epidermis 12,19 g, hepatopankreas 9,61 g, darah 1,06 g dan karapas 0,42 g. kandungan pigmen pada organ gonad memiliki nilai tertinggi dibandingkan dengan organ lainnya, diduga kepiting G. albolineatus betina ini berada pada tahap matang gonad, sehingga pigmen karotenoid masih tertumpuk pada organ gonad yang digunakan untuk proses pematangan gonad. Jenis-jenis pigmen yang teridentifikasi pada ekstrak organ karapas, lapisan epidermis, hepatopankreas, gonad dan darah dari kepiting G. albolineatus betina dengan larutan pengembang PE dan Aseton (80:20) yang bersifat semipolar yaitu: β-karoten, ekinenon, kantaksantin, tipe adonirubin, astaksantin dan astasen.Kata kunci : Grapsus albolineatus, KLT, pigmen

scholarly journals ISOLASI PIGMEN KAROTENOID DARI KEPITING Grapsus sp. (CAROTENOID PIGMENTS ISOLATION FROM CRABS Grapsus sp.)

2019 ◽  
Vol 7 (2) ◽  
pp. 104
Author(s):  
Dias Diasasthisa ◽  
Darus J Paransa ◽  
Desy MH Mantiri ◽  
Antonius Rumengan ◽  
Veibe Warouw ◽  
...  
Keyword(s):  
Vitamin A ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Column Chromatography ◽  
Photosynthetic Bacteria ◽  
Carotenoid Pigments ◽  
Carotenoid Pigment ◽  
Bacteria And Fungi ◽  
Layer Chromatography ◽  
Β Carotene

Carotenoids are pigments with a range of red, orange and yellow colors. In carotenoid and chlorophyll plants are located in chloroplasts which undergo photosynthesis as well as photosynthetic bacteria and fungi. Carotenoid compounds have antioxidant activity, anticancer, as precursors of vitamin A and can enhance immunity. Crustaceans like crabs Grapsus sp. have carotenoid pigments, visible red, orange, and yellow are scattered in the carapace. Pigments found in crustaceans as well as crabs of Grapsus sp. generally sourced from food eaten by the crab. To determine the process of carotenoid pigment metabolism, each crab organ of Grapsus sp. isolated using thin layer chromatography separation method and column chromatography to determine the type of pigment contained therein. Pigment types identified in the crabs of Grapsus sp. males using the TLC method: β-carotene, Astasen type, Cantasantin, Astasantin, and Adonirubin. Pigment types identified in the crabs of Grapsus sp. males using the CC method: β-carotene, Astasen, β-cryptosanthine, Zeaxantine, and Cryptosanthine. Keywords : Grapsus sp., Thin Layer Chromatography, Column Chromatography, Carotenoids Pigments.             Karotenoid merupakan pigmen dengan kisaran warna merah, orange dan kuning. Pada tumbuhan karotenoid dan klorofil terletak pada kloroplas yang mengalami proses fotosintesis seperti juga pada bakteri fotosintetik dan fungi. Senyawa karotenoid memiliki aktivitas antioksidan, antikanker, sebagai prekursor vitamin A dan dapat meningkatkan imunitas. Krustasea seperti kepiting Grapsus sp. mempunyai pigmen karotenoid, terlihat warna merah, jingga, dan kuning yang tersebar pada karapas. Pigmen yang terdapat pada krustasea demikian juga pada kepiting Grapsus sp. umumnya bersumber dari makanan yang dimakan oleh kepiting tersebut. Untuk mengetahui proses metabolisme pigmen karotenoid maka masing-masing organ kepiting Grapsus sp. diisolasi dengan menggunakan metode pemisahan kromatografi lapis tipis dan kromatografi kolom untuk mengetahui jenis pigmen yang terkandung didalamnya. Jenis pigmen yang teridentifikasi pada kepiting Grapsus sp. jantan dengan metode KLT yaitu : β- karoten, Tipe Astasen, Kantasantin, Astasantin, dan Adonirubin. Jenis pigmen yang teridentifikasi pada kepiting Grapsus sp. jantan dengan metode KK  yaitu : β- karoten, Astasen, β-kriptosantin, Zeaxantin, dan Kriptosantin. Kata Kunci : Grapsus sp., Kromatografi Lapis Tipis, Kromatografi Kolom, Pigmen Karotenoid.  

scholarly journals Analysis Of Types Of Carotenoid Pigments In Crab Sesarmops sp From Manado By Coast

2021 ◽  
Vol 9 (2) ◽  
pp. 204
Author(s):  
Melinda Margareta Adrian ◽  
Darus S. J Paransa ◽  
James J. H Paulus ◽  
Nickson J. Kawung ◽  
Robert A. Bara ◽  
...  
Keyword(s):  
Column Chromatography ◽  
Carotenoid Pigments ◽  
Carotenoid Pigment ◽  
Chromatographic Methods ◽  
Total Pigment ◽  
Male Crab ◽  
Yellow Orange ◽  
Pigment Extract ◽  
Mangrove Crabs ◽  
Layer Chromatography

Carotenoid pigments are a group of pigments that are yellow, orange, and red-orange in color. Pigments are natural dyes found in plants and animals, extracts of carotenoid pigments can be separated by chromatographic methods where the common chromatographic methods in determining the type of pigment are Column Chromatography (CC) and Thin Layer Chromatography (TLC). The purpose of this study was to determine the types of pigments contained in the carapace extract of the male crab Sesarmops sp. The Sesarmops sp crab has a brown dorsal carapace with blackish-brown leg spots known as mangrove crabs. Crabs in the Sesarmidae family have a carapace formation and wide legs, do not have swimming legs, and have a pair of claws that are faded purple, the presence of these colors can be identified as containing carotenoid pigments. The results of this study obtained the content of carotenoid pigment 25.2 g/gr and the value of the concentration of carotenoid pigment in the male crab extract Sesarmops sp 10.99 g. The results of the separation of the total pigment extract using column chromatography obtained the types of pigments -carotene, Ekinenon, Zeaxanthin, and Astaxanthin. Keywords: Column Chromatography (CC); Carotenoids; Sesarmops sp                                                                       AbstrakPigmen karotenoid adalah sekelompok pigmen yang berwarna kuning, oranye dan merah oranye. Pigmen adalah zat warna alami yang terdapat pada tumbuhan  dan hewan, ekstrak pigmen karotenoid dapat dipisahkan dengan metode kromatografi yang dimana metode kromatografi umum dalam menentukan jenis pigmen adalah Kromatografi Kolom (KK) dan Kromatografi Lapis Tipis (KLT). Tujuan dari penelitian ini untuk mengetahui jenis-jenis pigmen apa saja yang terdapar pada ekstrak karapas kepiting jantan Sesarmops sp. Kepiting Sesarmops sp memiliki karapas dorsal bewarna coklat dengan bintik kaki coklat kehitaman yang dikenal sebagai kepiting mangrove. Kepiting dalam keluarga sesarmidae memiliki bentukan karapas dan kaki yang lebar tidak memiliki kaki renang serta memiliki sepasang capit berwarna ungu pudar, adanya warna tersebut kepiting dapat diidentikasikan mengandung jenis pigmen karotenoid.  Dari hasil penelitian ini mendapatkan kandungan pigmen karotenoid 25,2 dan nilai konsentrasi pigmen karotenoid pada ekstrak kepiting jantan Sesarmops sp 10,99 . Hasil pemisahan dari ekstrak pigmen total menggunakan kromatografi kolom didapatkan jenis pigmen β-karoten, Ekinenon, Zeaxantin dan Astaxantin

scholarly journals EKSTRAKSI PIGMEN KAROTENOID PADA CANGKANG KEPITING SEBAGAI PEWARNA ALAMI YANG SEHAT

2018 ◽  
Vol 7 (1) ◽  
pp. 27
Author(s):  
Nasriani Nasriani
Keyword(s):  
Industrial Waste ◽  
Room Temperature ◽  
Extraction Process ◽  
Natural Dye ◽  
Provitamin A ◽  
Solvent Ratio ◽  
Carotenoid Pigments ◽  
Carotenoid Pigment ◽  
Pigment Type ◽  
Β Carotene

Extraction of carotenoid pigments from crab shells is a step to create healthy natural dyes through the utilization of industrial waste. Because, crab shells contain various types of carotenoid pigments that serve as provitamin A and antioxidants for the body.This research used maseration method by using silent solvent at room temperature. In the extraction process used 2: 1 solvent ratio with the shell then stored in a freezer temperature -20oC then applied to sausage and onde-onde food products. The resulting carotenoid pigment was analyzed using a spectrophotometer. The results showed that in 1kg of shell produced 531.25 mL / kg dye of carotenoid pigment consisting of pigment type astaxanthin, lutein, lycopene, β-carotene and cantaxanthin which served as provitamin A and antioxidant. Therefore the natural dye of the potent crab shells is used as a healthy natural dye. Ekstraksi pigmen karotenoid dari cangkang kepiting merupakan suatu langkah untuk menciptkan pewarna alami yang sehat melalui pemanfaatan limbah hasil industri. Sebab, cangkang kepiting mengandung berbagai jenis pigmen karotenoid yang berfungsi sebagai provitamin A dan antioksidan bagi tubuh.Penelitian ini menggunakan metode maserasi dengan menggunakan pelarut diam pada suhu ruangan. Dalam proses ekstraksi digunakan perbandingan pelarut 2 : 1 dengan cangkang kemudian disimpan dalam freezer bersuhu -20oC kemudian diaplikasikan pada produk makanan sosis dan onde-onde. Pigmen karotenoid yang dihasilkan dianalisa dengan menggunakan spektrofatometer.Hasil menunjukkan bahwa dalam 1kg cangkang dihasilkan 531,25 mL/Kg pewarna dari pigmen karotenoid yang terdiri dari jenis pigmen astaxanthin, lutein, likopen, β-caroten dan cantaxanthin yang berfungsi sebagai provitamin A dan antioksidan. Oleh karena itu pewarna alami dari cangkang kepiting potensil digunakan sebagai pewarna alami yang sehat.

scholarly journals Reproduction of the catfish Iheringichthys labrosus (Lütken) (Pisces, Siluriformes) in Furnas reservoir, Minas Gerais, Brazil

2004 ◽  
Vol 21 (2) ◽  
pp. 193-200 ◽  
Author(s):  
José Enemir dos Santos ◽  
Nilo Bazzoli ◽  
Elizete Rizzo ◽  
Gilmar Bastos Santos
Keyword(s):  
Feeding Activity ◽  
Gonadosomatic Index ◽  
Secretory Activity ◽  
Minas Gerais ◽  
Reproductive Period ◽  
Mature Stage ◽  
Hepatosomatic Index ◽  
Gonadal Maturation ◽  
Caudal Region ◽  
Iheringichthys Labrosus

The reproductive biology of Iheringichthys labrosus (Lütken, 1874) was studied in Furnas reservoir, Minas Gerais, Brazil. The fishes, 323 males and 817 females, were captured bimonthly from August 1993 to July 1994 by using gill nets with 3-10 cm mesh size. Gonadal maturation of the specimens was analyzed through macro and microscopical characteristics of the gonads. The secretory activity of the tubules of the caudal region of the testis was analyzed during the maturational testicular cycle. Females and males were in reproductive activity throughout the year in the reservoir. The peak of advanced maturation/mature stages occurred from October to January and of spawned/spent from February to May. The spawning of I. labrosus was identified as fractional or multiple type: occurring in the reservoir or in tributaries around it. The oocyte development of the species was asynchronous as different clusters of oocytes occurred simultaneously in the ovarian parenchyma. The epithelium of the tubules of the caudal region of the testis was in high secretory activity during advanced maturation/mature stage and this activity was absence in resting. The gonadosomatic index followed gonadal maturation, and the hepatosomatic index of females was lowest in advanced maturation/mature probably due the contribution of the liver in the vitellogenesis. The variations of the stomach repletion index, coelomic fat and condition factor indicated that the feeding activity was reduced and that the energetic reserves were consumed during the reproductive period.

Carotenoids in Lichens From Tasmanian Forests

1990 ◽  
Vol 38 (5) ◽  
pp. 517 ◽  
Author(s):  
B Czeczuga ◽  
G Kantvilas
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Total Content ◽  
Lichen Species ◽  
First Time ◽  
Layer Chromatography ◽  
Β Carotene

Column and thin-layer chromatography revealed the presence of the following carotenoids in the thalli of 20 lichen species from Tasmanian forests: α-carotene, β-carotene, β -cryptoxanthin, lutein, zeaxanthin, 5-hydroxy-5,6-dihydrozeaxanthin, β -carotene epoxide, lutein epoxide, antheraxanthin,mutatoxanthin, violaxanthin, neoxanthin, α-doradexanthin, canthaxanthin and astaxanthin. 5-Hydroxy-5,6-dihydrozeaxanthin is reported for the first time in lichens. The total content of carotenoids ranged from 15.28�g-1 dry wt in Menegazzia confusa to 48.53�g-1 dry wt in Pseudocyphellaria glabra.

Notizen:Untersuchungen über Carotinoide in Gametangien und Sporen von Allomyces arbuscula/ Studies on Carotenoids in Gametangia and Spores of Allomyces arbuscula

1977 ◽  
Vol 32 (5-6) ◽  
pp. 442-443
Author(s):  
P. Fähnrich ◽  
L. Chenaux
Keyword(s):  
Thin Layer ◽  
Visible Light ◽  
Thin Layer Chromatography ◽  
Allomyces Arbuscula ◽  
Lag Period ◽  
Development Analysis ◽  
Layer Chromatography ◽  
Β Carotene

Carotenoids were extracted from gametophytes of Allomyces arbuscula at different periods during gametogenesis. After separation by thin-layer chromatography γ-carotene, lycopene and β-carotene were identified from their absorp­tion spectra in visible light. No other carotenoids were detected. After a lag period of 30 min following induction of gametogenesis γ-carotene was the only carotenoid present in extracts, followed by traces of β-carotene at 60 min and by lycopene at 90 min. Accumulation of carotenoids continued during the later stages of gametangia development. Analysis of pigments that had been extracted from asexual mitospores showed that these spores contained both γ-carotene and lycopene.

Study of Coating Binder Migration and Affected Parameters

2013 ◽  
Vol 790 ◽  
pp. 45-48 ◽  
Author(s):  
Zhao Hui Zhen ◽  
Zheng Shun Wang
Keyword(s):  
Calcium Carbonate ◽  
Cross Section ◽  
Coating Layer ◽  
Solid Content ◽  
Pigment Content ◽  
Drying Temperature ◽  
Coating Surface ◽  
Pigment Type ◽  
Binder Concentration ◽  
Coating Color

Because of the coating binder migration in cross-section of coating layer, the binder concentration will be different in z-direction of coating layer, which affect some properties of coated paper. The Paper studied the effects of pigment type, coating color solid content and dying temperature on binder migration and affected factors. The z-direction distribution of the coating binder which was tagged with Br in coating layer and its concentration in coating surface were examined quantitatively by using SEM-EDXA and ESCA analysis. The mechanism of binder migration was also proposed. The results indicated that the degree of binder migration is directly proportional to the drying temperature, that the increase of the ratio of calcium carbonate in the pigment content, coating surface binder concentration increased. The result also indicated that the degree of binder migration was increased with reduced the coating solid content.

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