KONDISI KUALITAS AIR (ASPEK MIKROORGANISME) DI PERAIRAN SEKITAR PULAU BUNAKEN, SULAWESI UTARA

2017 ◽  
Vol 5 (3) ◽  
pp. 94
Author(s):  
Roberto V Larat ◽  
Markus T Lasut ◽  
Robert A Bara

In general, water conditions surrounding Bunaken Island are not separated from the liquid waste disposal, for example: bathing, washing, latrines, and landfills. Based on this, the research was conducted to determine the content of microorganisms Escherichia coli and coliform derived from liquid waste around Bunaken Island. The result showed the concentrations of E. coli were point (TS) I (<1 MPN / 100 ml), II (<1 MPN / 100 ml), and III (<1 MPN / 100 ml), and at the sampling point (TS) IV (1.0 MPN / 100 ml), V (1.0 MPN / 100 ml), and VI (1.0 MPN / 100 ml). Furthermore, coliform concentration has the similar valueat each sampling points (TS) I, II, III, IV, V, and VI with the amount of 2.0 MPN / 100 ml. The status around the Bunaken island on the comparison table between the this results and the standards of the Sea Quality Standard for Marine Tourism still qualifies or does not exceed the limits which stated in the creed of Minister ofEnviromental Affair Republic of Indonesia number 179 of 2004. Followed by Creed of Minister ofEnviromental Affair number 51 of 2004 on the Quality of Raw Water for Marine Biota, is categorized as the "proper" one of sea water quality for marine biota on the based ofEscherichia coli and coliform ratio.Keywords: Escherichia coli, coliform, air quality, Bunaken Island, North SulawesiPada umumnya, kondisi perairan yang berada di sekitar Pulau Bunaken tidak terlepas dari pembuangan limbah cair, misalnya : mandi, cuci dan kakus, serta tempat pembuangan sampah. Bertolak dari hal tersebut, penelitian ini dilakukan untuk mengkaji keberadaan kualitas limbah cair dalam aspek mikroorganisme di perairan sekitar pulau Bunaken dengan mengukur konsentrasi Escherichia coli dan coliform.Konsentrasi Escherichia coli berturut-turut pada titik sampling (TS) I (<1 MPN/100 ml), II (<1 MPN/100 ml) , dan III (<1 MPN/100 ml), dan pada titik sampling (TS) IV (1,0 MPN/100 ml), V (1,0 MPN/100 ml) , dan VI (1,0 MPN/100 ml). Coliformmemiliki jumlah konsentrasi yang sama, yaitu berturut-turut pada titik sampling (TS) I, II, III, IV, V, dan VI dengan jumlah 2.0 MPN/100 ml.Status perairan sekitar pulau Bunaken pada tabel perbandingan antara hasil pengukuran dan standart Baku Mutu Air Laut Untuk Wisata Bahari, masih memenuhi syarat atau tidak melewati batas yang ditetapkan dalam KEPMEN L.H. No.179 tahun 2004.Sedangkan berdasarkan KEPMEN L.H. No.51 Tahun 2004 tentang Baku Mutu Air Laut Untuk Biota Laut, berada dalam kategori “layak” sebagai peruntukan air laut untuk biota laut, berdasarkan parameter Escherichia coli dan coliform.Kata Kunci :Escherichia coli, coliform, kualitas air, Pulau Bunaken, Sulawesi Utara

2019 ◽  
Vol 19 (2) ◽  
pp. 1
Author(s):  
Etty Sabuin ◽  
Fonny Risamasu ◽  
Frans Kiaduan

Marine life and natural scenery - in the category of nautical tourism - are the potentials of the Kelapa Lima, Kupang beach. However, there are problems that; construction progress, residentials and bussines activities nearby coast of Kelapa Lima, Kupang, indicated that there are contamination by wastewater into the waters of this beach. Then, this issue is adjusted to the standards Environment Minister Regulation number 51 of 2014 and in 2013 number 115 on the sea water quality standard. The method used is the equation written by Sumiotomo and Nerow (1970) in the Minister of Environment No. 115 of 2003. The formula that is used to calculate the results of studies measuring the location parameter with the default value determined by the Ministry of Environment in 2004. Later, research shows that the quality of the waters of the Pantai Kelapa Lima Kupang has begun to decline in the presence of contamination by sewage. Liquid waste is already affecting marine life that corals and mangroves, while the beauty of the sea coast, the liquid waste is already reducing the amount of oxygen for the oxidation process that takes coastal sea to the decomposition of waste into the sea, causing sedimentation affect water clarity sea shore. Therefore, there needs to be immediate action from the government to educate more people about the importance of maintaining the beauty of the beach, and helped make the disposal of liquid waste discharges in coastal areas to reduce the causes of liquid wastes into the coastal sea.


2020 ◽  
Vol 20 (1) ◽  
pp. 1-6
Author(s):  
Andi Daramusseng ◽  
Syamsir Syamsir

Latar belakang : Degradasi kualitas perairan dapat terjadi akibat adanya zat pencemar yang mempengaruhi dan mengubah kondisi lingkungan perairan seperti Escherichia coli (E. coli). Tujuan dalam penelitian ini adalah untuk menganalisis kualitas air sungai Karang Mumus ditinjau dari parameter E. coli untuk Keperluan higiene sanitasi.Metode: Metode dalam penelitian ini adalah observasi dan  pemeriksaan laboratorium. Pengambilan sampel air dilakukan di sungai utama sebanyak tujuh titik. Penentuan titik pengambilan sampel air ini berdasarkan potensi sumber pencemar mulai dari hulu sampai ke hilir dengan kriteria terdapat daerah padat penduduk, peternakan, mall, hotel dan pasar. Teknik analisis yang digunakan yaitu dengan membandingkan hasil uji laboratorium dengan Peraturan Menteri Kesehatan Republik Indonesia Nomor 32 Tahun 2017 tentang Standar Baku Mutu Kesehatan Lingkungan dan Persyaratan Kesehatan Air untuk Keperluan Higiene Sanitasi, Kolam Renang, Solus Per Aqua, dan Pemandian Umum. Hasil: Hasil pengukuran Bakteri E. coli Sungai Karang Mumus, Kota Samarinda menunjukan bahwa kandungan bakteri E. coli terendah <30 CFU/100 mL dan yang tertinggi 2100 CFU/100 mLSimpulan:  Semua hasil pengukuran bakteri E. coli di Sungai Karang Mumus sudah melebihi baku mutu yang ditetapkan yaitu   0 CFU/100 mL sampel.  Langkah untuk meminimalkan kontaminan bakteri ke sungai perlu diambil sehingga penggunaan air Sungai Karang Mumus tidak membahayakan kesehatan masyarakat setempat. ABSTRACTTitle: Study on the Quality of the Karang Mumus River Water in terms of Escherichia coli Parameters For the Purpose of Hygiene SanitationBackground: Water quality degradation can occur due to the presence of pollutants that affect and change the condition of the aquatic environment such as Escherechia coli (E. coli). The purpose of this study was to analysis of Karang Mumus River Water quality in terms of Escherichia coli parameters for the purpose of hygiene sanitation. Method: The method in this research is observation and laboratory examination. Water sampling was taken in the main river for seven points. The determination of this water sampling point is based on potential sources of pollutants from upstream to downstream where there are densely populated areas, farms, malls, hotels, and markets. The analysis technique used is by comparing the results of laboratory tests with the Regulation of the Minister of Health of the Republic of Indonesia Number 32 the year 2017 concerning the Standards of Environmental Health Quality Standards and Water Health Requirements for Sanitary Hygiene, Swimming Pools, Solus Per Aqua, and Public Baths. Result: The measurement results of E. coli Bacteria in Karang Mumus River, Samarinda City showed that the lowest E.Coli bacteria content was <30 CFU / 100 mL and the highest was 2100 CFU / 100 mL.  Conclusion: All measurement results of E. coli bacteria in the Karang Mumus River have exceeded the established quality standard of 0 CFU / 100 mL sample. Steps to minimize bacterial contaminants to the river need to be taken so that the use of Karang Mumus river water does not endanger the health of the local community.


2021 ◽  
Vol 2 (1) ◽  
pp. 27-34
Author(s):  
Lizalidiawati Lizalidiawati ◽  
Erma Juniarti ◽  
Budi Harlianto

PLTU Teluk Sepang Bengkulu City produces heat waste dumped back into the sea which affects the quality of the water so that it has a negative impact on marine life. This study aimed to identify the quality of the water around PLTU Teluk Sepang Bengkulu City based on the physical and chemical parameters. The method used was direct measurement at the research location which is carried out at 12 measurement points,i.e 4 points around the outlet area, and 8 points in the off-shore area. The measurement result was compared with seawater quality standard for marine biota based on the Minister of Environment Decree No. 51 of 2004 and was validated by analysis of sea water quality before the PLTU had operated. The result showed that the some parameters met the quality standards for marine biota included DO of 5.62-7.82, pH and turbidity in the offshore area with each value of 7.24-7.86 and 1.68-4.846 NTU. Meanwhile, the parameters which did not meet quality standards include a temperature of 30.62-36 ºC, a salinity of 26.6-30 ‰,  pH and turbidity around the outlet area with each value of  6.7-6.88 and 23.25-146.8 NTU. Based on the validation result, it showed that heat waste had a very significant effect on the physical and chemical parameters of the waters, especially on temperature, pH, and turbidity.


2017 ◽  
Vol 13 (2) ◽  
pp. 111-119
Author(s):  
Lela Uyara ◽  
Pieter Kunu ◽  
Silwanus M Talakua

The study aims to determine the quality of clean water in the villages of Wainitu, Batumerah, Amahusu and Halong by comparing the result of water quality analysis with water quality standard. Water quality analysis includes Physiscal, Chemical, and Microbiological parameters. This research uses descriptive method, this method describes systematics, accurate about facts and characteristic of the quality of clean water of each research location. The results showed that the source of clean water in the village of Batumerah did not meet the standard of clean water quality standards indicated by the number of E. coli and the high total coliform.  Keywords: standard quality of clean water, water quality, Wainitu, Batumerah, Amahusu and Halong villages   ABSTRAK Penelitian yang bertujuan untuk menetapkan kualitas air bersih di Desa Wainitu, Batumerah, Amahusu dan Halong, dengan membandingkan hasil analisis kualitas air dengan standar baku mutu air bersih. Analisis kualitas air meliputi parameter fisika, kimia dan mikrobiologi. Penelitian ini menggunakan metode deskriptif; metode ini menggambarkan sicara sistematis, akurat, fakta dan karakteristik mengenai kualitas air bersih di masing-masing lokasi penelitian. Hasil penelitian menunjukkan bahwa sumber air bersih di Desa Batumerah tidak memenuhi standar baku mutu air bersih yang ditunjukkan oleh jumlah E. coli dan total Koliform yang tinggi. Kata Kunci: baku mutu air bersih, Desa Wainitu, Batumerah, Amahusu dan Halong, kualitas air


1975 ◽  
Vol 74 (3) ◽  
pp. 431-440 ◽  
Author(s):  
P. A. Ayres

SUMMARYIncidents of non-specific illness associated with the consumption of oysters have highlighted the lack of published information on the bacteriology of shellfish suitable for consumption. Investigations showed that the majority of molluscan shellfish entering English markets conform to the accepted standard of less than 5 Escherichia coli/ml. tissue. The numbers of E. coli were related to the sanitary quality of the growing area but no relation could be established between numbers of E. coli and coliforms, faecal streptococci or Clostridium welchii. The numbers of non-specific bacteria varied considerably but shellfish from sources associated with non-specific illness yielded relatively high counts at 37° C. The results showed that there was no justification for a standard based on total plate counts, which often exceeded 106/g. Such a standard would have to be coupled with spoilage or the incidence of non-specific illness. The relation between the numbers of non-specific bacteria growing at 20 and 37° C. appears to be a useful measure for assessing the likelihood that raw shellfish are a public health risk.


2021 ◽  
Vol 24 (5-esp.) ◽  
pp. 636-639
Author(s):  
Luana Romão Rodrigues ◽  
João Victor de Freitas Menezes ◽  
Dárcio Luiz de Sousa Júnior ◽  
Rafael de Carvalho Mendes

A cosmetologia é a ciência que engloba e estuda os cosméticos, desde conceitos iniciais até a sua fabricação, tornando-se uma ciência multidisciplinar. Para garantia de um uso seguro e eficaz dos cosméticos é necessário a realização de análises para se conseguir um padrão de qualidade. Quando há indícios de contaminação por microrganismos no cosmético, diversos problemas podem ser encontrados, como a ocorrência de patologias específicas, causando altos riscos e complicações à saúde do usuário. O objetivo deste trabalho foi investigar qualitativamente através de análises microbiológicas a qualidade dos produtos cosméticos de maquiagem usados de forma compartilhada, utilizando amostras colhidas aleatoriamente em uma loja de varejo, que foi selecionada por ser de grande circulação, da cidade de Juazeiro do Norte, Ceará. Nesta pesquisa foram analisadas oito amostras coletadas durante o período de outubro e novembro de 2017. As análises que foram realizadas seguiram a Farmacopeia brasileira. Como resultado obteve-se um percentual de 87,5 % de contaminação microbiana, evidenciando a presença de Staphylococcus aureus, Escherichia coli, Enterococcus sp. e Pseudomonas aeruginosa, sendo que apenas uma das oito amostras analisadas mostrou-se adequada para uso seguindo os limites de aceitação microbiológicas permitidos por lei. Baseados nesses resultados foi possível concluir que o índice de contaminação das amostras de batons que são utilizadas de modo compartilhado são potencialmente maiores. Com isso, intensifica-se a necessidade de medidas que impeçam o compartilhamento dos produtos cosméticos, com a finalidade de diminuir o risco de uma infecção coletiva.   Palavras-chave: Cosméticos. Uso compartilhado. Contaminação.   Abstract Cosmetology is the science that encompasses and studies cosmetics, from initial concepts to their manufacture, becoming a multidisciplinary science. To guarantee a safe and effective use of cosmetics it is necessary to carry out analyzes to achieve a quality standard. When there is evidence of contamination by microorganisms in the cosmetic, several problems can be found, such as the occurrence of specific pathologies, causing high risks and complications to the health of the user. The objective of this scientific work was to qualitatively investigate through microbiological analysis the quality of cosmetic makeup products used in a shared way, using samples collected randomly in a retail store, which was selected because it is of great circulation, in the city of Juazeiro do Norte, Ceará. In this research, eight (8) samples collected during the period of October and November 2017 were analyzed. The analyzes that were carried out followed the Brazilian Pharmacopoeia. As a result, a percentage of 87.5 % of microbial contamination was obtained, showing the presence of Staphylococcus aureus, Escherichia coli, Enterococcus sp. and Pseudomonas aeruginosa, with only one of the eight samples analyzed being suitable for use following the limits of microbiological acceptance allowed by law. Based on these results it was possible to conclude that the contamination index of the lipstick samples that are used in a shared way are potentially higher. As a result, the need for measures to prevent the sharing of cosmetic products is intensified, in order to reduce the risk of a collective infection.   Keywords: Cosmetics. Shared Use. Contamination.


2012 ◽  
Vol 10 (3) ◽  
pp. 243 ◽  
Author(s):  
Hanna Lethycia Wolupeck ◽  
Helen Caroline Raksa ◽  
Luciane Silvia Rossa ◽  
Raquel Biasi ◽  
Renata Ernlund Freitas de Macedo

O queijo Minas frescal é um dos mais populares do Brasil, porém o alto teor de umidade associado ao métodode processamento, muitas vezes artesanal, e de armazenamento desse produto o tornam muito perecível.Este trabalho teve como objetivo avaliar e comparar a qualidade microbiológica de queijo Minas frescalcomercializado na cidade de Curitiba (PR) nos anos de 1999 e 2009, verificando a evolução na qualidadehigiênico-sanitária desse produto no período de 10 anos. Foram analisadas 11 marcas comerciais de queijo Minas frescal disponíveis no comércio varejista da cidade de Curitiba, sendo amostradas cinco unidades de cada marca, totalizando 55 amostras. Os queijos foram submetidos à pesquisa de Salmonella spp., contagem de coliformes totais e Escherichia coli, contagem de Staphylococcus coagulase positiva e contagem de aeróbios mesófilos, com resultados expressos em UFC/g. Das 55 amostras de queijo, 41,82% e 78,18% apresentaram contagem de E. coli e de coliformes totais acima do limite permitido, respectivamente. Somente uma amostra (1,82%) do total avaliado mostrou-se em desacordo com os padrões para S. coagulase positiva e uma para Salmonella spp. Ambas as amostras foram adquiridas em 2009. Todas as amostras avaliadas em 2009 apresentaram elevada contagem de aeróbios mesófilos, revelando alta carga microbiana. Comparativamente, os queijos avaliados em 1999 mostraram qualidade microbiológica superior aos queijos avaliados em 2009 (p < 0,05). Destes, 100% apresentaram no mínimo um parâmetro microbiológico em desacordo com a legislação vigente, indicando que a qualidade dos queijos Minas frescal avaliados em 2009 apresentou-se inferior a dos queijos avaliados em 1999.


2020 ◽  
Vol 21 (7) ◽  
Author(s):  
Rahmad Lingga ◽  
Sri Budiarti ◽  
Iman Rusmana ◽  
Aris Tri Wahyudi

Abstract. Lingga R, Budiarti S, Rusmana I, Wahyu AT. 2020. Isolation, characterization and efficacy of lytic bacteriophages against pathogenic Escherichia coli from hospital liquid waste. Biodiversitas 21: 3234-3241. Escherichia coli is known as a pathogenic contaminant bacteria in hospital wastewater hazardous to humans and the environment. Concerns about the emergence of chlorine- and antibiotic-resistant bacteria increase the urgency to find an alternative strategy to control pathogenic bacteria in hospital wastewater. One of the alternatives is using lytic bacteriophage. This study aimed to isolate, characterize, and examine the efficacy of lytic bacteriophage against pathogenic Escherichia coli from hospital wastewater. It isolated, characterized (plaque morphology, host range, virion electron micrograph, and sensitivity to temperature, pH, and chlorine treatments), and tested the efficacy of lytic bacteriophages in controlling pathogenic E. coli isolated from hospital wastewater. Five phages were successfully obtained, all of which had clear plaques (lytic phage character). Based on host range assay, most of the phages could lyse all tested E. coli strains but not for other species. Electron micrograph photography revealed that the phages belonged to Myoviridae. The phages showed stability in high temperature, broad-ranged pH, and high concentrations of chlorine treatments. Assay on phages efficacy suggested that the phages are capable of significantly reducing the E. coli population both in sterilized and non-sterilized wastewater. The combination of phage treatment and chlorine was more effective than single phage treatment. The efficacy test revealed that phage application in wastewater had the best result seen from cocktail treatment and a combination of phage treatment and chlorine. These results suggested that the phage can be a potential candidate for disinfection purposes.


1977 ◽  
Vol 40 (11) ◽  
pp. 790-794 ◽  
Author(s):  
JAMES F. FOSTER ◽  
JAMES L. FOWLER ◽  
WARREN C. LADIGES

The microbiological quality of 150 units of raw ground beef obtained from a local retail store was determined. The range of aerobic plate counts was from 6.9 × 104 to 8.3 × 107/g. By using the most probable number method 96.7% of the 150 units were positive for coliforms, 94.7% for Escherichia coli and 61.3% for Staphylococcus aureus. By the plate methods, 99.3% of the units were positive for fecal streptococci and 56% were positive for Clostridium perfringens. No salmonellae were isolated. Aerobic and anaerobic organisms were isolated and identified. E. coli was the most frequently isolated aerobe followed by organisms in the Klebsiella-Enterobacter group. Among the anaerobic isolates, C. perfringens was the organism most frequently encountered.


2020 ◽  
Vol 36 (2) ◽  
pp. 75-77
Author(s):  
Anindita Bhowmik ◽  
Sunjukta Ahsan

Majority of the population of Bangladesh depend on tap or surface water as their source of water supply. This study was carried out to examine the microbial quality of both water and soil collected from different places using the multiple tube fermentation technique to determine coliform count by the most probable number (MPN) method in brilliant green lactose broth (BGLB) media.Inoculum from positive tubes of the presumptive test were further transferred on eosinemethylene blue (EMB) and MacConkey agar.The organisms isolated were further characterized using biochemical tests. Out of 93 water samples, 30 (32.26%) indicated the presence of lactose fermenter and gas producer in all 3 tubes of dilution series using inoculum quantities of 1.0, 0.1 and 0.01 ml, whereas out of 85 soil samples, 45 (52.94%) showed acid and gas production in all 3 tubes of dilution series.Among 85 soil samples, 40 samples that contained at least one positive in each dilution series and among 93 water samples, 31 samples that contained at least one positive in each dilution series were further re-identified with biochemical tests.This study showed 30.59% soil isolates and 26.88% water isolates were Escherichia coli which highlighted the fact that both water and soil act as a major reservoir of E.coli, which indicates possible fecal contamination as well as presence of potentially pathogenic E. coli. Bangladesh J Microbiol, Volume 36 Number 2 December 2019, pp 75-77


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