AKTIVITAS SENYAWA ANTIBAKTERI DAN ANTI-UV DARI Phyllidia varicosa (Cuvier, 1804) DAN BAKTERI SIMBIONNYA (NUDIBRANCHIA GASTROPODA) DARI PERAIRAN TANJUNG MANDOLANG, MINAHASA

Phyllidia varicosa is an organism belongs to the order Nudibranchia, which is commonly known as a sea rabbit. This organism is able to synthesize secondary metabolites from food. The purpose of this study was to obtain P. varicosa extract and symbiotic bacterial extract from P. varicosa, then determine the antibacterial activity of P. varicosa extract and the symbionic bacteria extract against Escherichia coli DSM498, Bacillus megaterium DSM32T and anti-UV activity. P. varicose symbiotic bacterial were isolated and extracted. The results obtained 5 bacterial isolates. The results of antibacterial assay of isolates PhVa 1.1, PhVa 1.3, PhVa 2.1, PhVa 2.3 and PhVa 2.4 shown that these isolates have an antibacterial activity against E. coli DSM498 and B. megaterium DSM32T. Anti-UV assay results shown an absorption at UV-A with the highest value of 1.991 at λ 340 nm. Keywords: Nudibranchia, Phyllidia varicosa, Antibacterial, Anti-UV, Escherichia coli, Bacillus megaterium Abstrak Phyllidia varicosa merupakan organisme yang termasuk dalam ordo Nudibranchia, yang umumnya dikenal sebagai kelinci laut. Organisme ini mampu mensintesis metabolit sekunder dari bahan makanannya. Tujuan dari penelitian ini yaitu untuk mendapatkan ekstrak P. varicosa dan ekstrak bakteri simbion dari P. varicosa, kemudian mengamati aktivitas antibakteri dari ekstrak P. varicosa dan ekstrak bakteri simbionnya terhadap bakteri Escherichia coli DSM498 dan Bacillus megaterium DSM32T serta menguji aktivitas anti-UV. Bakteri yang bersimbion dengan P. varicosa diisolasi dan diekstraksi, lalu diuji bioaktivitas antibakteri dan diuji anti-UV terhadap ekstrak P. varicosa dan ekstrak bakteri simbionnya. Hasil akhir dari penelitian ini yaitu didapatkan 5 isolat bakteri. Hasil uji aktivitas antibakteri yaitu isolat PhVa 1.1, PhVa 1.3, PhVa 2.1, PhVa 2.3 dan PhVa 2.4 memiliki aktivitas antibakteri terhadap bakteri E. coli DSM498 dan B. megaterium DSM32T. Hasil uji anti-UV menunjukkan serapan pada UV-A dengan nilai tertinggi 1,991 pada λ 340 nm. Kata kunci: Nudibranchia, Phyllidia varicosa, Antibakteri, Anti-UV, Escherichia coli, Bacillus megaterium

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UJI AKTIVITAS ANTIBAKTERI DAN ANTI-UV Phyllidiella nigra DAN BAKTERI SIMBIOTIKNYA DARI PERAIRAN TANJUNG MANDOLANG

JURNAL PESISIR DAN LAUT TROPIS ◽

10.35800/jplt.8.2.2020.29768 ◽

2020 ◽

Vol 8 (2) ◽

pp. 61

Author(s):

Tessalonica Dajoh ◽

Robert A Bara ◽

Esther Angkouw ◽

Medy Ompi ◽

Rosita A Lintang ◽

...

Keyword(s):

Escherichia Coli ◽

Antibacterial Activity ◽

Secondary Metabolites ◽

Bacillus Megaterium ◽

Absorption Maximum ◽

Chemical Compounds ◽

Inhibition Zone ◽

Defense System ◽

E Coli ◽

Self Defense

Phyllidiella nigra is an organism that is suspected to have secondary metabolites because their ability to develop its self defense system by camouflage and using chemical compounds derived from their nature diet as deterrent against their predators. The purpose of this study was to isolate symbiotic bacterial derived from P. nigra, extracted and followed by, the antibacterial assays against Escherichia coli and Bacillus megaterium as well as the anti-UV assay. The results showed that the five isolates tested had an antibacterial activity with the highest average inhibition zone against E. coli DSM 498 bacteria, isolate 1 (14.67 mm), isolate 5 (14 mm), and against B. Megaterium DSM 32T bacteria, isolate 3 (13.33 mm). The three isolates which had the highest inhibition zone and P. nigra extract were tested for anti-UV assay using a UV-Vis Spectrophotometer. The results obtained isolate 3 has absorption of UV-A with the UV absorbtion maximum at λ 340 nm and P. nigra extract has absorption on UV-B radiation with UV absorption maximum at λ 290 nm. Key words: Nudibranchia, Bacteria, Anti-bacteial, Anti-UV Phyllidiella nigra merupakan organisme yang diduga memiliki metabolit sekunder karena mampu mengembangkan sistem pertahanan dirinya dengan cara kamuflase dan menggunakan senyawa kimia sebagai racun yang didapat dari makanannya. Tujuan dari penelitian ini yaitu mendapatkan isolat bakteri yang bersimbiosis dengan P. nigra, mendapatkan ekstrak dari baktri simbion, dan menguji antibakteri dan anti-UV ekstrak etil aseta bakteri simbion dengan metode difusi agar terhadap bakteri Escherichia coli dan Bacillus megaterium. Hasil penelitian didapatkan kelima isolat yang diuji memiliki aktivitas antibakteri dengan rerata zona hambat tertinggi terhadap bakteri E. coli DSM 498 yaitu isolat 1 (14,67 mm), isolat 5 (14 mm), dan terhadap baktri B. megaterium DSM 32T yaitu isolat 3 (13,33 mm). Ketiga isolat yang memiliki zona hambat tertinggi dan ekstrak P. nigra diujikan anti-UV menggunakan alat UV-Vis Spektrofotometer. Hasil yang didapat isolat 3 memiliki serapan terhadap radiasi sinar UV-A dengan puncak tertinggi pada λ 340 nm dan ekstrak P. nigra memiliki serapan terhadap radiasi sinar UV-B dengan puncak tertinggi berada pada λ 290 nm. Kata kunci: Nudibranchia, Bacteria, Anti-bacteial, Anti-UV

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ISOLASI DAN SKRINING AKTIVITAS ANTIBAKTERI DARI BAKTERI SIMBION NUDIBRANCHIA Phyllidiella pustulosa dan Thuridilla lineolata

JURNAL PESISIR DAN LAUT TROPIS ◽

10.35800/jplt.8.2.2020.28768 ◽

2020 ◽

Vol 8 (2) ◽

pp. 40

Author(s):

Ade Kurniawan Arie ◽

Rosita A J Lintang ◽

Remy E P Mangindaan ◽

Agung B Windarto ◽

Fitje Losung ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Symbiotic Bacteria ◽

Bacterial Isolates ◽

Defense Systems ◽

Self Defense ◽

Solid Media ◽

Antibacterial Assay ◽

And Staphylococcus Aureus

Nudibranchs are marine invetebrates belongs to the class of Gastropoda that are able to camouflage and develop a self-defense systems. Marine bacteria contain chemicals compunds that have potentials on marine drugs discovery through the secondary metabolism. The purpose of this study was to isolate the symbiotic bacteria from nudibranches Phylidiella pustulosa and Thuridilla lineolate and to screen the antibacterial activity of these bacterial isolates against Escherichia coli and Staphylococcus aureus. Isolation and culture of the symbiotic bacteria was made on B1 (solid) media. The results of the antibacterial assay showed that the symbiotic bacterial isolates from Phylidiella pustulosa and Thuridilla lineolate were able to inhibit the growth of the Staphylococcus aureus and Escherichia coli test. Keywords: nudibranch, Phyllidiella pustulosa, Thuridilla lineolate, bacteria, antibacterialAbstrakNudibranch adalah avetebrata laut dalam kelas Gastropoda yang mampu melakukan kamuflase dan mengembangkan sistem pertahanan diri. Bakteri laut mengandung senyawa kimia yang berpotensi sebagai bahan obat-obatan yang dihasilkan melalui metabolit sekunder. Tujuan dari penelitian ini yaitu untuk mengisolasi bakteri yang bersimbion dengan nudibranch Phylidiella pustulosa dan Thuridilla lineolate, kemudian mengamati aktivitas antibakteri dari isolat bakteri tersebut terhadap Escherichia coli danStaphylococcus aureus. Isolasi dan kultur bakteri yang bersimbion dibuat pada media B1 (padat). Skrining aktivitas antibakteri menunjukkan isolat bakteri yang bersimbion dengan nudibranch Phylidiella pustulosa dan Thuridilla lineolate mampu menghambat pertumbuhan organisme uji Staphylococcus aureus dan Escherichia coli. Kata kunci: nudibranch, Phyllidiella pustulosa, Thuridilla lineolate, bakteri, antibakteri

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Penapisan antibakteri pada Bakteri Simbion Sinularia sp terhadap Escherichia coli

Rekayasa ◽

10.21107/rekayasa.v10i2.3609 ◽

2017 ◽

Vol 10 (2) ◽

pp. 87

Author(s):

Ary Giri Dwi Kartika

Keyword(s):

Escherichia Coli ◽

Antibacterial Activity ◽

Secondary Metabolites ◽

Dilution Method ◽

Conservative Approach ◽

Bacterial Isolates ◽

Inhibitory Zone ◽

Activity Test ◽

Overlay Method ◽

Wet Weight

karang lunak) memiliki metabolit sekunder dengan konsentrasi yang rendah (10-6% dari berat basa invertebrata). Oleh karena itu dibutuhkan cara yang lebih konservatif dalam pemanfaatan metabolit sekunder untuk menjaga keseimbangan ekosistem.Tujuan dari penelitian ini adalah mengisolasi bakterisimbion Sinularia sp dan melakukan penapisan aktivitas antibakteri terhadap Escherichia coli. Isolasi bakteri simbion Sinularia sp dilakukan dengan menggunakan metode pengenceran dan sebar (spread). Purifikasi bakteri menggunakan metode streak, kemudian, uji antibakteri dilakukan dengan menggunakan metode overlay dan difusi agar. Sebanyak 5 isolat bakteri didapatkan dari hasil isoasi sampel. Hasil uji aktivitas antibakteri menunjukkan sebanyak 4 isolat (Isolat L2.2, L2.3, L2.4, dan L2.5) memiliki aktivitas antibakteri terhadap bakteri Escherichia coli. Isolat L2.5 memiliki diameter zona hambat terbesar yaitu sebesar 2,207 ± 0,401 cm.Kata Kunci: Sinularia sp, Antibakteri, Escherichia coli, Gili Labak.Skrinning Antibacterial from Sinularia sp Symbiont Bacteria Againts Escherichia coliABSTRACTSeveral studies have shown that invertebrates (including soft corals) contain secondary metabolites in low concentration (10-6% by wet weight of invertebrate). Therefore a more conservative approach is needed in the utilization of secondary metabolites to maintain the balance of ecosystems. The purposes of this study was to isolate the bacterium Sinularia sp and to screen antibacterial activity against Escherichia coli. Isolation of the bacteria symbione of Sinularia sp was performed by dilution method and spread. Purification of bacteria was performed by streak metho then, antibacterial test was done by using overlay method and agar disk-diffusion. A total of 5 bacterial isolates were obtained from the result of the isoation of the sample. The result of antibacterial activity test showed 4 isolates (Isolates L2.2, L2.3, L2.4, and L2.5) had antibacterial activity against Escherichia coli. Isolates L2.5 has the largest inhibitory zone diameter of 2.207 ± 0.401 cm.Keywords: Sinularia sp, Antibactery, Escherichia coli, Gili Labak.

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Synthesis of Chalcones and Nucleosides Incorporating [1, 3, 4]Oxadiazolenone Core and Evaluation of their Antifungal and Antibacterial Activities

Current Bioactive Compounds ◽

10.2174/1573407214666180911130110 ◽

2020 ◽

Vol 15 (6) ◽

pp. 665-679

Author(s):

Alok K. Srivastava ◽

Lokesh K. Pandey

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Bacillus Subtilis ◽

Antifungal Activity ◽

Aspergillus Niger ◽

Gram Negative ◽

E Coli ◽

Gram Positive Bacteria ◽

Good Antibacterial Activity

Background: [1, 3, 4]oxadiazolenone core containing chalcones and nucleosides were synthesized by Claisen-Schmidt condensation of a variety of benzaldehyde derivatives, obtained from oxidation of substituted 5-(3/6 substituted-4-Methylphenyl)-1, 3, 4-oxadiazole-2(3H)-one and various substituted acetophenone. The resultant chalcones were coupled with penta-O-acetylglucopyranose followed by deacetylation to get [1, 3, 4] oxadiazolenone core containing chalcones and nucleosides. Various analytical techniques viz IR, NMR, LC-MS and elemental analysis were used to confirm the structure of the synthesised compounds.The compounds were targeted against Bacillus subtilis, Staphylococcus aureus and Escherichia coli for antibacterial activity and Aspergillus flavus, Aspergillus niger and Fusarium oxysporum for antifungal activity. Methods: A mixture of Acid hydrazides (3.0 mmol) and N, Nʹ- carbonyl diimidazole (3.3 mmol) in 15 mL of dioxane was refluxed to afford substituted [1, 3, 4]-oxadiazole-2(3H)-one. The resulted [1, 3, 4]- oxadiazole-2(3H)-one (1.42 mmol) was oxidized with Chromyl chloride (1.5 mL) in 20 mL of carbon tetra chloride and condensed with acetophenones (1.42 mmol) to get chalcones 4. The equimolar ratio of obtained chalcones 4 and β -D-1,2,3,4,6- penta-O-acetylglucopyranose in presence of iodine was refluxed to get nucleosides 5. The [1, 3, 4] oxadiazolenone core containing chalcones 4 and nucleosides 5 were tested to determined minimum inhibitory concentration (MIC) value with the experimental procedure of Benson using disc-diffusion method. All compounds were tested at concentration of 5 mg/mL, 2.5 mg/mL, 1.25 mg/mL, 0.62 mg/mL, 0.31 mg/mL and 0.15 mg/mL for antifungal activity against three strains of pathogenic fungi Aspergillus flavus (A. flavus), Aspergillus niger (A. niger) and Fusarium oxysporum (F. oxysporum) and for antibacterial activity against Gram-negative bacterium: Escherichia coli (E. coli), and two Gram-positive bacteria: Staphylococcus aureus (S. aureus) and Bacillus subtilis(B. subtilis). Result: The chalcones 4 and nucleosides 5 were screened for antibacterial activity against E. coli, S. aureus and B. subtilis whereas antifungal activity against A. flavus, A. niger and F. oxysporum. Compounds 4a-t showed good antibacterial activity whereas compounds 5a-t containing glucose moiety showed better activity against fungi. The glucose moiety of compounds 5 helps to enter into the cell wall of fungi and control the cell growth. Conclusion: Chalcones 4 and nucleosides 5 incorporating [1, 3, 4] oxadiazolenone core were synthesized and characterized by various spectral techniques and elemental analysis. These compounds were evaluated for their antifungal activity against three fungi; viz. A. flavus, A. niger and F. oxysporum. In addition to this, synthesized compounds were evaluated for their antibacterial activity against gram negative bacteria E. Coli and gram positive bacteria S. aureus, B. subtilis. Compounds 4a-t showed good antibacterial activity whereas 5a-t showed better activity against fungi.

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Proteomic response of Escherichia coli to a membrane lytic and iron chelating truncated Amaranthus tricolor defensin

BMC Microbiology ◽

10.1186/s12866-021-02176-4 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Tessa B. Moyer ◽

Ashleigh L. Purvis ◽

Andrew J. Wommack ◽

Leslie M. Hicks

Keyword(s):

Escherichia Coli ◽

Antibacterial Activity ◽

Antimicrobial Peptides ◽

Mechanism Of Action ◽

Gram Negative Bacteria ◽

Amaranthus Tricolor ◽

Core Motif ◽

Gram Negative ◽

E Coli ◽

Membrane Lysis

Abstract Background Plant defensins are a broadly distributed family of antimicrobial peptides which have been primarily studied for agriculturally relevant antifungal activity. Recent studies have probed defensins against Gram-negative bacteria revealing evidence for multiple mechanisms of action including membrane lysis and ribosomal inhibition. Herein, a truncated synthetic analog containing the γ-core motif of Amaranthus tricolor DEF2 (Atr-DEF2) reveals Gram-negative antibacterial activity and its mechanism of action is probed via proteomics, outer membrane permeability studies, and iron reduction/chelation assays. Results Atr-DEF2(G39-C54) demonstrated activity against two Gram-negative human bacterial pathogens, Escherichia coli and Klebsiella pneumoniae. Quantitative proteomics revealed changes in the E. coli proteome in response to treatment of sub-lethal concentrations of the truncated defensin, including bacterial outer membrane (OM) and iron acquisition/processing related proteins. Modification of OM charge is a common response of Gram-negative bacteria to membrane lytic antimicrobial peptides (AMPs) to reduce electrostatic interactions, and this mechanism of action was confirmed for Atr-DEF2(G39-C54) via an N-phenylnaphthalen-1-amine uptake assay. Additionally, in vitro assays confirmed the capacity of Atr-DEF2(G39-C54) to reduce Fe3+ and chelate Fe2+ at cell culture relevant concentrations, thus limiting the availability of essential enzymatic cofactors. Conclusions This study highlights the utility of plant defensin γ-core motif synthetic analogs for characterization of novel defensin activity. Proteomic changes in E. coli after treatment with Atr-DEF2(G39-C54) supported the hypothesis that membrane lysis is an important component of γ-core motif mediated antibacterial activity but also emphasized that other properties, such as metal sequestration, may contribute to a multifaceted mechanism of action.

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ANTIBACTERIAL AND ANTIOXIDANT ACTIVITY OF SAPONIN FROM ABUTILON INDICUM LEAVES

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2016.v9s3.15064 ◽

2016 ◽

Vol 9 (9) ◽

pp. 344 ◽

Cited By ~ 1

Author(s):

Lokesh Ravi ◽

Manasvi V ◽

Praveena Lakshmi B

Keyword(s):

Escherichia Coli ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Gas Chromatography Mass Spectrometry ◽

Diffusion Method ◽

E Coli ◽

Ms Analysis ◽

Antibacterial Assay ◽

Crude Saponin ◽

Abutilon Indicum

ABSTRACTObjective: Aim of this study is to analyze the antibacterial and antioxidant potential of crude saponin extract (CSE) from Abutilon indicum leaves.Methods: CSE was subjected for gas chromatography-mass spectrometry (GC-MS) analysis to identify its components. Antibacterial potentialwas analyzed using agar well diffusion method and minimum inhibitory concentration (MIC) was detected using 96-well plate method, againstStaphylococcus aureus (MTCC: 3160) and Escherichia coli (MTCC: 443). DNA damage study was performed using comet assay. Antioxidant capabilitywas studied using 2,2-diphenyl-1-picrylhydrazyl scavenging assay.Results: GC-MS analysis suggested a library match to benzene-1-4-bis(phenylmethyl), with a molecular weight of 258 g/mol to be the majorcomponent in the CSE at 21.25 RT. CSE demonstrated 96.16% free radical scavenging activity at 2.5 mg/ml concentration. CSE demonstrateda significant antibacterial activity in the well diffusion assay, S. aureus 17 mm and E. coli 15 mm, with a MIC value of 1.11 mg/ml. Comet assaydemonstrated no DNA damage.Conclusion: These results conclude that CSE of A. indicum leaves possesses promising antibacterial and antioxidant potential.Keywords: Abutilon indicum, Saponin, Escherichia coli, Staphylococcus aureus, 2,2-diphenyl-1-picrylhydrazyl, Antibacterial assay.

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The Structure and Antibacterial Activity of Taurine and its Magnesium Complex

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.366.404 ◽

2011 ◽

Vol 366 ◽

pp. 404-407

Author(s):

Li Hua Wang

Keyword(s):

Escherichia Coli ◽

Antibacterial Activity ◽

Bacillus Subtilis ◽

Single Crystal ◽

Dilution Method ◽

Monoclinic Space Group ◽

X Ray Diffraction ◽

X Ray ◽

Antibacterial Assay ◽

Serial Dilution Method

The block single-crystals of taurine were obtained, and its structure was determined by single-crystal X-ray diffraction. The single-crystal X-ray analysis of taurine reveals that the crystal belongs monoclinic, space group P2(1)/c with a = 0.52824(10) nm, b = 1.1647(8) nm, c = 0.79236(13) nm, ß = 94.0850(10). The magnesium complex with taurine has been synthesized in ethanol. The antibacterial assay of the Mg (II) complex was measureed using a modified version of the 2-fold serial dilution method. The results show that the complex shows considerable antibacterial activity against escherichia coli, bacillus subtilis and staphylococcus white.

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Antibacterial activity of Escherichia coli from squid ink (Loligo sp.) n-Hexane extracts

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/869/1/012033 ◽

2021 ◽

Vol 869 (1) ◽

pp. 012033

Author(s):

D R Utami ◽

I Irwan ◽

S Agustina ◽

S Karina ◽

S Afriani

Keyword(s):

Escherichia Coli ◽

Antibacterial Activity ◽

Secondary Metabolite ◽

Bioactive Compound ◽

Inhibition Zone ◽

Hexane Extract ◽

Inhibition Zone Diameter ◽

E Coli ◽

Hexane Extracts ◽

Intermediate Concentration

Abstract Squid is one of the export commodities in Indonesia. In general, the use of squid meat, while the ink is only as waste. In fact, Squid ink contain bioactive compound that potential as anti-inflammatory, antihypertensive, anti-diabetic,anti-microbial and anti-malaria agents. The purpose of the study is to determine the types of secondary metabolite compounds contained in n-hexane extract of Loligo sp. ink using maceration method to determine its antibacterial activity against Escherichia coli. The results of secondary metabolite compounds obtained from the n-hexane extract of Loligo sp. ink are alkaloid, saponins, glycosides and phenol. The results of antibacterial test against E. coli using the disc method obtained the average of inhibition zone diameter at the concentration of 4% is 6.3 mm (intermediate), concentration of 8% is 7.83 mm (intermediate), concentration of 16% is 14.5 mm (susceptible) and concentration of 32% is 10.83 mm (intermediate). The antibacterial activity in n-hexane extract of Loligo sp. ink is optimal at the concentration of 16% against E. coli bacteria.

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Identification and Probiotic Potency Screening of Cellulolytic Bacilli Isolated from Fecal Pellets and Gastrointestinal Tract of Nypha Worm (Namalycastis rhodochorde), West Kalimantan, Indonesia

Jurnal Biologi Indonesia ◽

10.47349/jbi/17022021/189 ◽

2021 ◽

Vol 17 (2) ◽

pp. 189-195

Author(s):

TR Setyawati ◽

AH Yanti ◽

R. Kurniatuhadi

Keyword(s):

Escherichia Coli ◽

Gastrointestinal Tract ◽

Organic Acids ◽

Acid Tolerance ◽

Distilled Water ◽

Bacterial Identification ◽

Bacterial Isolates ◽

Fecal Pellets ◽

West Kalimantan ◽

E Coli

The bacterial isolates NrLtF1, NrLtF4, NrLtF5, and NrLtG2 isolated from fecal pellets and gastrointestinal tract of nypha worms (Namalycastis rhodochorde) have cellulolytic, proteolytic activity and produce organic acids. The four isolates have the potency to be developed as probiotics in nypha worm cultivation feed. This study aims to determine the probiotics potency and identify the species of NrLtF1, NrLtF4, NrLtF5, and NrLtG2 isolate based on 16srDNA sequence. The probiotic potency was carried out by the acid tolerance assays on distilled water and 0.3% acid bile media, and the antimicrobial testing against Escherichia coli (MF exp21.12). Bacterial identification was carried out by sequencing of 16sDNA sequence based on GeneBank data. The results showed that the bacterial isolates of NrLtF1, NrLtF4, NrLtF5, and NrLtG2 were able to grow on 0.3% distilled water and acid bile media. However, only the NrLtF4 and NrLtF5 inhibited E. coli (MF exp21.12) with halo zones 30 mm and 18 mm, respectively. Blasting results of the 16srDNA sequences showed that the NrLtF1, NrLtF4, NrLtF5, and NrLtG2 were closely related to Bacillus wiedmannii, Brevibacterium sediminis, Bacillus proteolyticus, and Bacillus paramycoides. The nypha worm bacterial isolates have the potency to be developed as probiotics in nypha worm culture.

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Phytochemical analysis and antibacterial activity of Khaya senegalensis bark extracts on Bacillus subtilis, Escherichia coli and Proteus mirabilis

International Journal of Phytomedicine ◽

10.5138/09750185.1784 ◽

2016 ◽

Vol 8 (3) ◽

pp. 333 ◽

Cited By ~ 1

Author(s):

Abdullahi Aliyu ◽

Alkali BR ◽

Yahaya MS ◽

Garba A ◽

Adeleye SA ◽

...

Keyword(s):

Escherichia Coli ◽

Antibacterial Activity ◽

Bacillus Subtilis ◽

Proteus Mirabilis ◽

Traditional Healers ◽

Phytochemical Analysis ◽

Aqueous Extracts ◽

E Coli ◽

Khaya Senegalensis ◽

Ethanol Extracts

The aqueous and ethanol extracts of the bark of Khaya senegalensis were screened for their phytochemical constituents and preliminary antibacterial activity against Bacillus subtilis, Escherichia coli and Proteus mirabilis. The minimum inhibitory concentration (MIC) of the plant on the tested organisms was determined using multiple tubes method.Alkaloids, anthraquinones, glycosides, tannins and steroids were detected in both extracts.The ethanol and aqueous extracts of the plant showed antibacterial activity against B. subtilis and E. coli, with the aqueous extracts having more activity than those of ethanol. However the growth of P. mirabilis was not inhibited by either of the extracts. The MIC value was determined to be 50 mg/ml for B. subtilis and E. coli. The results are suggestive of considerable antibacterial activity of K. senegalensis and may justify its use in the treatment of bacterial diseases by herbalists or traditional healers.

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