scholarly journals RESULTS OF DETERMINING THE SPECIES COMPOSITION OF VAGINAL LACTOBACILLI USING REAL-TIME PCR TESTING AND BACTERIOLOGICAL METHOD

2018 ◽  
pp. 17-21
Author(s):  
E. S. Voroshilina ◽  
D. L. Zornikov ◽  
L. G. Boronina
Keyword(s):  
Species Composition ◽  
Real Time ◽  
Real Time Pcr ◽  
Culture Technique ◽  
Reproductive Age ◽  
Clinical Material ◽  
Women Of Reproductive Age ◽  
Vaginal Lactobacilli ◽  
Increased Risk ◽  
Before And After

Aim. To compare the species composition of lactobacilli in women of reproductive age using real time PCR on urogenital samples before and after culturing microorganisms in Blood-Serum-Y^ast extract-Agar (BSYA). Materials and methods. Using real time PCR, we have examined the species composition of vaginal lactobacilli in 25 healthy women of reproductive age. Samples of urogenital swabs (endocervical and vaginal swabs) were studied. Species identification has been carried out twice: in the native clinical material and in the samples received after using microorganism culture technique in BSYA. Results. After culturing vaginal microorganisms in BSYA, L. iners predominance was found only in 2 cases (8%). At the same time, when using native clinical material this species predominance was discovered in 10 samples (40%). Conclusion. When culturing lactobacilli in BSYA, the growth of L. iners is stunted compared to other species of vaginal lactobacilli. The use of cultural testing turned out to be ineffective in case of predominance of L. iners, the species associated with an increased risk of vaginal dysbiosis.

scholarly journals Detection of thyroid peroxidase mRNA and protein in orbital tissue

2006 ◽  
Vol 155 (2) ◽  
pp. 213-218 ◽  
Author(s):  
Oi Fah Lai ◽  
Norazean Zaiden ◽  
Sui Sin Goh ◽  
Nur-Ezan Mohamed ◽  
Lay Leng Seah ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Real Time ◽  
Real Time Pcr ◽  
Protective Factor ◽  
Thyroid Peroxidase ◽  
Fat Tissue ◽  
Cultured Fibroblasts ◽  
Orbital Fat ◽  
Increased Risk ◽  
Before And After

Objective: We have previously reported that the absence of thyroid peroxidase antibodies (TPOAb) in Graves’ disease (GD) was associated with an increased risk of Graves’ ophthalmopathy (GO). This observation raised the possibility that TPOAb could act as a protective factor. However, the presence of thyroid peroxidase (TPO) in the orbit has not been previously reported. The aim of this study was to confirm or exclude the presence of orbital TPO. Methods and design: Relative TPO mRNA expression from GO (n = 6) and normal (n = 5) orbital fat tissue was determined using real-time PCR technique. Orbital fat in the normal group from blepharoplasty represents extraconal (anterior) fat. mRNA expression in fibroblasts grown from these tissues before and after adipocyte differentiation was also documented. Finally, Western blotting was carried out to verify translation of TPO mRNA transcripts. Results and discussion: TPO transcripts were detected in the orbital fat tissue obtained from normal and GO subjects using the real-time PCR technique. TPO expression was increased in GO compared to normal (N) tissues. However, TPO expression in cultured fibroblasts was similar in both groups and adipogenesis did not appear to alter TPO expression. Protein was detected by Western blot analysis using the TPO MAB 47 (mAb 47). The predicted 110-kDa band was detected in orbital fat as well as in orbital fibroblasts. Our results suggest the presence of TPO in GO and N orbital tissues. We hypothesise that immune responses directed against orbital TPO might play a role in modulating the clinical expression of GO.

scholarly journals Iodine Excretion and Intake in Women of Reproductive Age in South Australia Eating Plant-Based and Omnivore Diets: A Pilot Study

2021 ◽  
Vol 18 (7) ◽  
pp. 3547
Author(s):  
Jane S. Whitbread ◽  
Karen J. Murphy ◽  
Peter M. Clifton ◽  
Jennifer B. Keogh
Keyword(s):  
Pilot Study ◽  
Urinary Iodine ◽  
Group Versus ◽  
Iodine Intake ◽  
Reproductive Age ◽  
World Health ◽  
Urinary Iodine Concentration ◽  
Women Of Reproductive Age ◽  
Increased Risk ◽  
Iodine Excretion

Women consuming a strictly vegan/plant-based diet may be at increased risk of low iodine intake due to avoidance of animal products containing iodine. The aim of this pilot study was to determine the iodine excretion and intake in women consuming vegan/plant based diets compared with women consuming omnivore diets. Fifty-seven women (n = 31 plant-based, n = 26 omnivores), provided two spot urine samples to assess urinary iodine concentration (UIC). Two days of dietary intake were also recorded by participants. As the data were not normally distributed results are reported as median (IQR). UIC was significantly different between groups, 44 (26–66) µg/L in the vegan/plant-based group versus 64 (40–88) µg/L in omnivores (p < 0.05). UIC did not meet the >100 µg/L level recommended by the World Health Organization. Iodine intake was also significantly different, 78 (62–91) µg/day in the vegan/plant-based group and 125 (86–175) µg/day in the omnivores (p = 0.000). Iodine intake and bread intake were correlated with iodine excretion (CC 0.410–4.11, p = 0.003). These data indicate iodine insufficiency in both groups of women as the median values were below the minimum WHO recommendation. A larger study assessing iodine excretion in the Australian women of reproductive age who are not pregnant or breastfeeding is needed to confirm these findings.

scholarly journals OVARIAN MALIGNANCY

2018 ◽  
Vol 25 (02) ◽  
pp. 237-241
Author(s):  
Shabana Rafiq ◽  
Razia Bibi ◽  
Samina Ashraf
Keyword(s):  
Cross Sectional Study ◽  
Reproductive Age ◽  
Ovarian Malignancy ◽  
Ovarian Mass ◽  
Sectional Study ◽  
Women Of Reproductive Age ◽  
Cross Sectional ◽  
Increased Risk ◽  
History Of ◽  
Study Setting

Objectives: To determine the frequency of ovarian malignancy in women ofreproductive age presenting with ovarian mass and to determine frequency of factors leadingto ovarian malignancy. Study Design: Cross sectional study. Setting: Department of Obstetrics& Gynaecology, Lady Wallington Hospital, Lahore. Period with Dates: From 05.11.11 to26.06.12. Results: The result of our study reveals majority of the patients between 21-30 yearsi.e. 45.88%(n=39), common age was 24.21+3.76 years, 43.53%(n=37) were nulliparous (inmajority), while frequency of ovarian malignancy in women of reproductive age presenting withovarian mass was recorded in 14.11%(n=12) while frequency of factors leading to ovarianmalignancy was 91.67%(n=11) patients were nulliparous while family history of ovarianmalignancy was in 8.33%(n=1) patients. Conclusions: The frequency of ovarian malignancy ishigher among reproductive age females with increased risk of nulliparity.

scholarly journals Evaluation of a single-tube real-time PCR for detection and identification of 11 dermatophyte species in clinical material

2010 ◽  
Vol 16 (6) ◽  
pp. 704-710 ◽  
Author(s):  
A.M.C. Bergmans ◽  
M. van der Ent ◽  
A. Klaassen ◽  
N. Böhm ◽  
G.I. Andriesse ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Clinical Material ◽  
Single Tube ◽  
Detection And Identification ◽  
Dermatophyte Species

scholarly journals Prevalence of Anemia and Its Associate Factors among Women of Reproductive Age in Lao PDR: Evidence from a Nationally Representative Survey

Anemia ◽  
2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Sengtavanh Keokenchanh ◽  
Sengchanh Kounnavong ◽  
Akiko Tokinobu ◽  
Kaoru Midorikawa ◽  
Wakaha Ikeda ◽  
...  
Keyword(s):  
Public Health ◽  
Logistic Regression ◽  
Associated Factors ◽  
Lao Pdr ◽  
Reproductive Age ◽  
Major Public Health Problem ◽  
Women Of Reproductive Age ◽  
Sanitation Facility ◽  
Increased Risk ◽  
Prevalence Of Anemia

Introduction. Anemia continues to be a major public health problem significant among women of reproductive age (WRA) in developing countries, including Lao People’s Democratic Republic (Lao PDR), where the prevalence of anemia among women remains high. This study aimed to assess the prevalence of anemia and its associated factors among WRA 15–49 years in Lao PDR. Methods. We conducted a cross-sectional study, using the Lao Social Indicator Survey II, 2017 dataset. A total of 12,519 WRA tested for anemia were included in this study, through multistage sampling approaches. Binary logistic regression was used to determine the associated factors of anemia. Results. Of 12,519 women, 4,907 (39.2%) were anemic. Multivariate logistic regression revealed that living in central provinces (aOR: 2.16, 95% CI: 1.96–2.38), rural area (aOR: 1.1, 95% CI: 1.00–1.20), large family size with more than 6 persons (aOR: 1.14, 95% CI: 1.01–1.29), pregnancy (aOR: 1.46, 95% CI: 1.22–1.74), having any adverse pregnancy outcomes (aOR: 1.14, 95% CI: 1.03–1.25), poor drinking water (aOR: 1.24, 95% CI: 1.10–1.39), and poor sanitation facility (aOR: 1.15, 95% CI: 1.03–1.28) were significantly associated with an increased risk of anemia. Conversely, four factors were associated with anemia preventively, including being aged 25–34 years (aOR: 0.81, 95% CI: 0.74–0.90), postsecondary education (aOR: 0.76, 95% CI: 0.60–0.97), Hmong-Mien ethnicity (aOR: 0.48, 95% CI: 0.39–0.59), and watching television almost daily (aOR: 0.84, 95% CI: 0.75–0.95). Conclusion. Anemia continues to be a major public health challenge in Lao PDR. Interventions should be considered on geographic variations, improving safe water and sanitation facility, promoting of iron supplements during pregnancy, and health education through mass media for women in rural areas.

scholarly journals Real-time PCR for laboratory diagnosis of Acanthamoeba keratitis

2011 ◽  
Vol 32 (2) ◽  
pp. 111
Author(s):  
Joanna Cheng ◽  
Ian Carter ◽  
Liping Wang ◽  
Peter Taylor
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Hot Springs ◽  
Tap Water ◽  
Dna Amplification ◽  
Culture Method ◽  
Laboratory Diagnosis ◽  
Acanthamoeba Keratitis ◽  
Culture Technique ◽  
Human Cornea

Acanthamoeba keratitis is a painful vision-threatening disease of the human cornea. It is characterised by severe ocular pain or partial paracentral stromal ring infiltrate, which can be frequently misdiagnosed as herpes simplex virus keratitis. If the infection is not treated promptly, it may progress to ulceration of the cornea, loss of visual acuity, possibly blindness and even require enucleation. Acanthamoeba sp are found commonly in freshwater, tap water, seawater, hot springs and swimming pools. An epidemiologic case study revealed that major risk factors were the use of contact lenses, predominantly extended-wear soft lenses, the use of homemade rinsing saline and users who wear their lenses while swimming. The conventional method of detecting the formation of oocysts of Acanthamoeba by a culture technique takes an average three?five days. DNA amplification by PCR can improve turnaround time for the diagnosis. A study was carried out in this laboratory to compare the traditional culture method with a real-time PCR assay.

scholarly journals DETEKSI DINI KANKER SERVIKS DENGAN PEMERIKSAAN SERVIKS DI KELURAHAN BULURAN KENALI KOTA JAMBI TAHUN 2017

2019 ◽  
Vol 1 (1) ◽  
pp. 56
Author(s):  
Julaecha Julaecha ◽  
Nurfitriani Nurfitriani
Keyword(s):  
Cervical Cancer ◽  
Early Detection Of Cancer ◽  
Malignant Disease ◽  
Intraepithelial Neoplasia ◽  
Cervical Cancer Prevention ◽  
Reproductive Age ◽  
Pap Smears ◽  
Women Of Reproductive Age ◽  
Before And After ◽  
Implementation Method

Cervical cancer is a malignant disease that occurs in the cervik. Course of the disease is preceded by the condition of pre-concerous lesions of the cerviks is the existence of dysplasi/cervical intraepithelial neoplasia (NIS). On each day is expeced to 40-45 new cases and about 20-25 people  die from cervical cancer. Based on those considered very necessary to provide education  on cervical cancer in women of reproductive age in the prevention of cervical cancer prevention effort.Target outcomes expected are: there is an increased knowledge an increase in knowledge between before and after extension and capable of early detection of cancer  serviks increase. Implementation method to provide counseling.The results  showed that there was important of mother’s interest in doing pap smears examination before and after counseling.

scholarly journals HERV-K18 Env Expression and the Potential Association With TGF-β and Epstein–Barr Virus Infection in the Presence and the Absence of Vitamin D in Multiple Sclerosis Patients

2021 ◽  
Author(s):  
Tayebeh Latifi ◽  
Majid Teymoori-Rad ◽  
Ahmad Nejati ◽  
Shohreh Shahmahmoodi ◽  
Farhad Rezaei ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Vitamin D ◽  
Real Time ◽  
Real Time Pcr ◽  
Epstein Barr Virus Infection ◽  
Healthy Controls ◽  
Increased Risk ◽  
Multiple Sclerosis Patients ◽  
Vitro Effect

Abstract Previous studies have reported that Human endogenous retroviruses (HERVs) such as HERV-K18 is associated with an increased risk of multiple sclerosis (MS). The present study aimed to evaluate the association between the expression of HERV-K18 env, TGF-β, and in vitro effect of EBV infection on the expression level of HERV-K18 env in PBMC of MS patients in both presence and absence of vitamin D (1,25-OHD). The levels of HERV-K18 env was measured in peripheral blood mononuclear cells (PBMCs) from 20 MS patients and 20 healthy controls by quantitative real-time PCR. PBMCs were further treated with EBV in the presence or the absence of vitamin D. After 72 hours, cells were collected for measurement of HERV-K18 env expression using Real-time PCR. While the expression of HERV-K18 env was significantly higher in MS patients than healthy controls, the expression of TGF–β was found to be significantly lower in MS patients compare to healthy controls. Interestingly, an inverse correlation was found between HERV-K18 env expression and TGF-β expression in MS patients but not for healthy controls. Although in vitro stimulated PBMCs with EBV showed no significant differences in terms of HERV-K18 expression, EBV infected cells revealed different patterns in the presence and the absence of vitamin D treatment. These findings not only support the important role of HERV-K18 env expression but also highlight the plausible interactions with different risk factors in MS.

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