Ultramicroscopic structure of peyer’s patches in the rats’ small intestine after imunofan correction of experimental immunosuppression

2021 ◽  
Vol 22 (2) ◽  
pp. 36-39
Author(s):  
Е. N. Morozova ◽  
◽  
V. N. Morozov ◽  
S. V. Zabolotnaya ◽  
T. A. Mikhailik ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Standard Technique ◽  
Uranyl Acetate ◽  
Peyer's Patches ◽  
Granular Endoplasmic Reticulum ◽  
Male Rats ◽  
Control Group ◽  
Peyer’S Patches ◽  
Stromal Component ◽  
Group 1

Aim. To study the structure of Peyer’s patches at the ultramicroscopic level after the administration of imunofan against the background of experimentally modeled immunosuppression caused by the administration of cyclophosphamide. Materials and methods. The study was carried out on 36 white mature male rats, divided into 2 groups. The Group 1 included rats, which on the 1st day of the experiment were injected with cyclophosphamide at a dose of 200 mg/kg, and then imunofan according to the scheme on days 2, 4, 6, 8, 10 at a dose of 0.7 μg/kg of body weight. Group 2 was formed from rats, which were injected with an equivalent amount of isotonic sodium chloride solution. Animals of the Group 1 were Taken out from the experiment on the days 7 and 30 after the correction of cyclophosphamide-induced immunosuppression with imunofan. Pieces of Peyer’s patches were isolated and processed according to a standard technique. Ultrathin sections were contrasted in a solution of uranyl acetate and lead citrate according to E. Reynolds and studied under an electron microscope with further photographing. Results. It was found that lymphocytes, in most cases, are tightly adjacent to each other on the days 7 and 30 after the correction of cyclophosphamide-induced immunosuppression with imunofan; in the control group, the stroma is more often located between them. In the nucleus of these cells, heterochromatin is located in clumps and adjoins the nuclear membrane; in the control group, it fills two-thirds of the karyoplasm. The cells with patterns of mitosis, lymphoblasts are often found, macrophages contain large lysosomes on days 7 and 30 of experiment, compared with the control group of rats. In plasmocytes, the granular endoplasmic reticulum occupied up to 2/3 of the cell cytoplasm. The stromal component is well expressed in the control group of animals, compared with the experimental one, and is represented by reticulocytes, reticular fibers and vessels, and fibroblastic reticular cells are also found. Conclusion. In an ultramicroscopic study, the administration of the immunomodulator imunofan against the background of cyclophosphamide-induced immunosuppression promoted an increase in lysosomes in macrophages, lymphoblasts, cells with mitotic patterns, as well as the development of a granular endoplasmic reticulum on days 7 and 30 of the experiment, compared with the control group.

scholarly journals Planimetric correlations between Peyer’s patches and the area of small intestine of white rats

2018 ◽  
Vol 24 (2) ◽  
pp. 66-72 ◽  
Author(s):  
V.H. Hryn
Keyword(s):  
Small Intestine ◽  
White Male ◽  
Peyer's Patches ◽  
Male Rats ◽  
Control Group ◽  
Peyer’S Patches ◽  
White Rats ◽  
Longitudinal Length ◽  
Significant Difference ◽  
Lymphoid Nodules

The largest concentration of the local immune system in the form of the nodular associations of the lymphoid tissue with the epithelium of the mucous membranes (tonsils, single lymphoid nodules and their aggregations, named Peyer’s patches) is located in the gut. The paper is aimed at the study of the quantitative and planimetric correlations between the Peyer’s patches and the area of the small intestine of white rats based on the visual assessment and comparative analysis of some metric values. The study was conducted on 60 white male rats, which were assigned into 2 groups of 30 animals: after the morning feeding (controls) and after a daily fasting. After the removal made in advance, the gut-associated complexes have been studied from the stomach to the cecum. The resulting experimental data have been processed on a personal computer using the EXCEL 2010 (Microsoft Excel Corp., USA) software. No significant difference in the diameter and length of the small intestine of white rats of the first and second groups was found. The total area of the small intestine wall in the control group ranged from 8666 mm2 to 20724 mm2, and from 8496 mm2 to 20573 mm2 in group II (after a daily fasting), ranking equally. Thickness parameters of the small intestine in two groups of animals were almost similar in its unchanged, within the limits of individual variability, longitudinal length. To conduct an accurate quantitative and planimetric analysis of the aggregated lymphoid nodules of the white rat’s small intestine, it was advisable to distinguish 3 groups, namely: small-, medium- and large-sized. Thus, their total number varies from 12 to 28 units. Among them 8 to 17 units (on the average of 12.60±0.400) were small-sized, 2 to 11 (5.800±0.500) were medium-sized, and the large ones were not always found. For example, in the studied samples, they were absent in 6 animals, whereas 1 to 5 units were presented in the rest of animals. The area of single small Peyer’s patches ranged from 1.570 to 9.800 mm2, and their total area was 64.90±2.900 mm2; the area of medium samples individually ranges from 10.60 to 27.50 mm2. Totally, they occupy an average area of 97.60±8.00 mm2. The same value of individual large aggregated nodules is between 31.40 and 60.40 mm2, which totally accounted on the average of 58.40±10.30 mm2. Thus, the average statistical value of the total areas of Peyer’s patches is 220.9±14.40 mm2, accounting for only 2% of the total area of the small intestine wall of white rats, not including single lymphoid nodules, not exceeding 1 mm.

scholarly journals Effects of Hexachlorocyclohexane (HCH-γ-Isomer, Lindane) Intoxication on the Proliferation and Apoptosis in Rat Testes

2009 ◽  
Vol 78 (4) ◽  
pp. 615-620 ◽  
Author(s):  
Hayati Yuksel ◽  
Erkan Karadas ◽  
Hikmet Keles ◽  
Hasan Huseyin Demirel
Keyword(s):  
Germ Cell ◽  
Germ Cells ◽  
Male Rats ◽  
Control Group ◽  
High Dose ◽  
Histopathological Changes ◽  
Proliferation And Apoptosis ◽  
Group A ◽  
Higher Doses ◽  
Group 1

In this study, experimentally lindane-induced histopathological changes and proliferation and/or apoptosis in germ cells in the rat testes were investigated. A total of 40 healthy fertile 3-month-old male rats were used. Animals were divided into 4 groups, each containing 10 rats. Group 1 (control) was given only pure olive oil, Groups 2, 3 and 4 were administered lindane at 10, 20 and 40 mg/kg/bw, respectively, by gastric gavage for 30 days. Microscopically, degenerative changes were observed in the lindane-treated groups. For proliferative activity PCNA immunolabelling and for germ cells apoptosis TUNEL methods were performed. Although a strong PCNA positivity in the control group was observed, a gradual decrease was noted in the lindane-treated groups especially at higher doses. Significant increases of apoptosis were seen in the lindane-treated groups compared to the control group. A decrease in testosterone concentrations was observed in lindane-treated groups compared to the control group. The study indicates that high-dose lindane intoxication contributes to the suppression of spermatogenesis through a reduction of germ cell proliferation and an increase of germ cell death in rat testes.

scholarly journals Reactive and destructive changes of Peyer’s patches in rats with experimental burn disease under infusion of detoxification solutions

2019 ◽  
Vol 25 (2) ◽  
pp. 56-63
Author(s):  
V.G. Cherkasov ◽  
R.M. Matkivska ◽  
E.V. Cherkasov ◽  
R.F. Kaminskyi ◽  
L.M. Yaremenko
Keyword(s):  
Endoplasmic Reticulum ◽  
Dendritic Cells ◽  
Intravenous Infusion ◽  
Burn Injury ◽  
Peyer's Patches ◽  
Peyer’S Patches ◽  
Experimental Burn ◽  
Isotonic Sodium Chloride ◽  
Hyperosmolar Solutions ◽  
Burn Disease

The pathogenesis of burn immune dysfunction and burn enteropathy needs further clarification given that the cellular lesions of lymphoid tissue associated with mucous membranes are the least studied. The purpose of the study was to establish reactive and destructive changes in Peyer’s patches of rats after burn injury of the skin with the use of intravenous infusion of isotonic sodium chloride solution and combined colloid-hyperosmolar solutions. White male rats weighing 160-200 g at 6 months of age were divided into 4 groups (18 animals in each group): I, II, III – rats with burn skin injury (grade II-III burn with an area of 23% of body surface area and the development of moderate-severity shock state) which was administered a separate intravenous infusion once a day for the first 7 days of the experiment with isotonic sodium chloride, lactoprotein with sorbitol and HAES-LX-5%, in each case at a dose of 10 ml/kg; IV – intact animals. The material was collected from rats under deep thiopental intraperitoneal anesthesia after 1, 3, 7, 14, 21 and 30 days after burn injury. Biopsies from Peyer’s patches for histological and electron microscopic examination were processed using conventional methods. Investigation of histological preparations stained with hematoxylin-eosin was performed on an Olympus BX51 microscope. Ultrathin sections were contrasted on copper support meshes with uranyl acetate and lead citrate according to Reynolds and studied using a PEM-125K electron microscope. Electron and light microscopy data indicate that intravenous infusion of colloidal-hyperosmolar solutions (lactoprotein with sorbitol and HAES-LX-5%) promotes suppression of inflammatory response, inhibits necrosis, and optimizes lymphoid apoptosis at Peyer’s patches of rats with experimental burn disease caused by burn injury to the skin of 21-23% of the body surface. Apoptotic lymphocytes and their apoptotic bodies are effectively phagocytosed by macrophages and are digestible in heterophagolysosomes. The apoptotic altered dendritic cells in Peyer’s patches are characterized by osmiophilic cytoplasm and a nucleus with high electron density amorphous nucleoplasm. In the cytoplasm are located mitochondria with enlightened matrix and destroyed cristae, irregularly expanded tubules of variable configuration of a granular endoplasmic reticulum with electronically transparent content and numerous derivatives of their vacuole transformation, which are sharply darkened. The fusion of vacuoles leads to the formation of large electron-luminous cavities filled with various residues of compacted degraded cellular structures. Vacualization promotes site segmentation of condensed cytoplasm of apoptotic dendritic cells and formation of apoptotic blebs, which are subject to entrapment and subsequent sequential degradation with the participation of neighboring macrophages. The structural changes of the organelles of the protein-synthesizing apparatus found in Peyer’s plaque cells in rats with experimental burn disease can be regarded as a manifestation caused by functional overload of the granular endoplasmic reticulum (ER-stress). The consequence of optimal development of ER-stress and subsequent unfolded protein response is the apoptotic degradation of the corresponding cell, the course of which is modified by the use of colloid-hyperosmolar solutions.

scholarly journals EFFECT OF IMUNOPHAN ON MORPHOMETRIC PARAMETERS AND HISTOLOGICAL STRUCTURE OF PEYER'S PATCHES IN SMALL INTESTINE OF RATS UNDER IMMUNOSUPPRESSION INDUCED BY CYCLOPHOSPHAN

2021 ◽  
pp. 141-152
Author(s):  
E.N. Morozova ◽  
V.N. Morozov ◽  
A.V. Tverskaya
Keyword(s):  
Small Intestine ◽  
Lymph Nodes ◽  
White Male ◽  
Morphometric Parameters ◽  
Peyer's Patches ◽  
Male Rats ◽  
Histological Structure ◽  
Peyer’S Patches ◽  
Group 2 ◽  
Intact Rats

The aim of the study is to analyze morphometric parameters and histological structure of Peyer's patches in the small intestine of rats after imunofan administration under immunosuppression caused by cyclophosphan. Materials and Methods. The study was carried out on 36 white male rats (210–250 gramms). The animals were divided into two groups. Rats of Group 1 were injected with cyclophosphamide on the 1st day of the experiment (200 mg/kg). On the 2nd, 4th, 6th, 8th and 10th days they received imunofan (0.7 μg/kg). Group 2 consisted of intact rats. The animals were withdrawn from the experiment on the 7th, 30th, and 90th days after imunofan administration. The authors measured the length and width of Peyer's patches, and the distance from the ileocecal angle to the first patch. We also examined the height and width of lymph nodes and internodal zones on histological sections. Results. On the 7th and 30th days of our study the length and width of Peyer's patches increased by 18.71 %/16.94 % and 3.56 %/24.22 % respectively in mature animals, compared with the intact rats. At the same time, the distance from the ileocecal angle to the 1st Peyer’s patch decreased by 6.15 % and 19.80 %. The micrography showed that the height and width of the lymph nodes of Peyer's patches in experimental animals increased by 29.79 %/12.73 % (Day 7) and 10.49 %/12.46 % (Day 30) in comparison with the intact rats. The linear sizes of internodal zones increased by 39.35 %/10.33 % (Day 7) and 4.77 %/4.25 % (Day 30). By the 90th day, the macroscopic parameters of Peyer's patches, the height and width of their lymph nodules and internodal zones approxomated those of intact animals. Conclusion. When using imunofan under experimental immunosuppression, macro- and micromorphometric parameters of Peyer's patches of the small intestine of mature rats gradually approximated those in intact rats, which indicated the leveling of cyclophosphan immunosuppressive effect. Key words: rats, small intestine, Peyer's patches, imunofan, cyclophosphan. Цель исследования – изучение морфометрических параметров и гистологического строения пейеровых бляшек тонкой кишки крыс после введения имунофана на фоне иммуносупрессии, вызванной циклофосфаном. Материалы и методы. Исследование проводилось на 36 белых крысах-самцах массой 210–250 г. Животные были разделены на две группы. В I группе крысам в 1-е сут эксперимента вводился циклофосфан в дозе 200 мг/кг, на 2, 4, 6, 8, 10-е сут – имунофан в дозе 0,7 мкг/кг массы тела; II группу составили интактные крысы. Животные выводились из эксперимента на 7, 30, 90-е сут после завершения введения имунофана. Измерялись длина и ширина пейеровых бляшек, а также расстояние от илеоцекального угла до первой из них, а на гистологических срезах – высота и ширина их лимфатических узелков и межузелковых зон. Результаты. У половозрелых животных на 7-е и 30-е сут наблюдения длина и ширина пейеровых бляшек увеличивалась на 18,71 и 16,94 % и 3,56 и 24,22 % соответственно по сравнению с данными интактных крыс, а расстояние от илеоцекального угла до первой из них уменьшалось на 6,15 и 19,80 %. При микроскопическом исследовании высота и ширина лимфатических узелков пейеровых бляшек были больше по сравнению с данными интактных крыс на 29,79 и 12,73 % (7-е сут), 10,49 и 12,46 % (30-е сут), а линейные размеры межузелковых зон – на 39,35 и 10,33 % (7-е сут), 4,77 и 4,25 % (30-е сут). К 90-м сут макроскопические параметры пейеровых бляшек, а также высота и ширина их лимфатических узелков и межузелковых зон приближались к данным интактных животных. Выводы. При использовании имунофана на фоне экспериментальной иммуносупрессии значения исследуемых макро- и микроморфометрических параметров пейеровых бляшек тонкой кишки половозрелых крыс в ходе эксперимента постепенно приближались к аналогичным показателям интактных крыс, что свидетельствует о нивелировании иммуносупрессивного эффекта циклофосфана. Ключевые слова: крысы, тонкая кишка, пейеровы бляшки, имунофан, циклофосфан.

scholarly journals Phytoremediation Potential of Maná-Cubiu (Solanum sessiliflorumDunal) for the Deleterious Effects of Methylmercury on the Reproductive System of Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Raquel Frenedoso da Silva ◽  
Gabriela Missassi ◽  
Cibele dos Santos Borges ◽  
Eloísa Silva de Paula ◽  
Maria Fernanda Hornos Carneiro ◽  
...  
Keyword(s):  
Leydig Cells ◽  
Sperm Morphology ◽  
Body Weight Gain ◽  
Sperm Concentration ◽  
Treated Group ◽  
Male Rats ◽  
Control Group ◽  
Sperm Production ◽  
Histological Aspect ◽  
Group 1

Methylmercury, organic form of mercury, can increase the number of abnormal sperm and decrease sperm concentration and testosterone levels possibly due to the damage caused by reactive species to germ and Leydig cells. Maná-cubiu (Solanum sessiliflorumDunal) is a native fruit from Amazon rich in iron, zinc, niacin, pectin, and citric acid, used in foods, beverages, and medicinal purposes, since it has been useful for treatment of various diseases caused by oxidative stress or nutritional deficiency. Therefore, this study evaluated the phytoremediation potential of this fruit on damages caused by exposure to MeHg on sperm quantity and quality and the histological aspect of the testis and epididymis. Wistar male rats (n=20) were randomly allocated into four groups: Control group (received distilled water), MeHg group (140 μg/Kg),Solanumgroup (1% of fruit Maná-cubiu on chow), andSolanumplus MeHg group (same treatment as MeHg and Solanum group). The organs were weighted, histopathology; sperm morphology and counts were obtained. The results showed reduction in body weight gain, testis weights, reduced sperm production, and increased histopathological abnormalities in the MeHg-treated group. However, treatment withSolanumplus MeHg revealed a protective effect of this fruit on damages caused by MeHg.

scholarly journals A Comparative Evaluation of Selected Medicinal Plants on Male Fertility Indices (Reproductive Hormones and Sperm Profile) of Albino Wistar Rats: An Animal Case Study

2019 ◽  
pp. 1-6
Author(s):  
Oliver E. Ngwu ◽  
J. I. Okoye
Keyword(s):  
Medicinal Plants ◽  
Reproductive Hormones ◽  
Male Rats ◽  
Mucuna Pruriens ◽  
Control Group ◽  
Albino Rats ◽  
High Dose ◽  
Velvet Bean ◽  
Testosterone Concentration ◽  
Group 1

The study aims to investigate the effects of the medicinal plants {seeds} on the  reproductive hormones and sperm profile of male albino rats to ascertain their possible usefulness  as  fertility agent. Walnuts [Tetracarpidium conophorum], Sesame (Sesamine indicum), and Velvet bean (Mucuna pruriens) seeds were obtained and taken to the Department of plant  Science and  Biotechnology, University of  Nigeria, Nsukka (UNN). The seeds were milled into fine powder. One hundred and ten sexually matured aibino rats of about twelve weeks weighing 130-180 g were divided into eleven groups (1-11) using completely randomized design. There were two different control groups and rats in group 1 served as the Control 1 and were fed with normal commercial feed. Rats in group 2 were administered with a drug (Ketoconazole) to induce infertility. The Infertility Induced groups were treated with low dose (groups 3-5), medium dose (groups 6-8) and  high dose (9-11) for the period of nine (9) weeks. At the end and testes and Epididymides were surgically removed and weighed. Blood  sample analysis revealed that the concentration of sex hormones  measured in the male rats fed with medium plants (seeds) showed that the testosterone concentration significanty increased (p<0.05) in animal control group 1(normal rats) Therefore,  these medicinal plants walnut seeds showed significant increase in their testosterone concentration, luteinizing hormone (LH) and follicle stimulating hormone (FSH) which significantly enhanced the production of reproductive hormones which enriched the fertility status of these animals.

scholarly journals The Сharacter and Development of photoreceptors photodegeneration combined with alloxan diabetes

2002 ◽  
Vol 1 (2) ◽  
pp. 47-52
Author(s):  
S. V. Logvinov ◽  
E. Yu. Varakuta ◽  
A. V. Potapov
Keyword(s):  
Diabetes Mellitus ◽  
Alloxan Diabetes ◽  
Light Exposure ◽  
Early Stage ◽  
Male Rats ◽  
Control Group ◽  
Group 3 ◽  
Group 2 ◽  
Alloxan Injection ◽  
Group 1

The aim of the research is to determine the character and development of a alterations in photoreceptors, caused by high-intensity light on the early stage of diabetes mellitus. The methods of light and electron microscopy were used. Experiments were obtained from 80 adult white male rats. The rats of group 1 (n = 20) received 1 injection of alloxan (15 mg per 100 g of weight) in order to model diabetes mellitus. The rats of group 2 (n = 20) were exposed for light of luminecent LB-40 lamps (6000 lx) for 6 hours. The rats of group 3 (n = 20) were exposed to the light with the same parameters 1 month after alloxan injection. The last 20 rats made a control group 4. The rats groups 2 and 3 were sacrifised by decapitation 1, 7, 14, 30 days after the light exposure, the rats of group 1 on the 4, 5, 6, 8 week after the alloxan injection. Light exposure combined with diabetes causes destruction pigment epithelium inner and outer processed of photoreceptors followed by pyknosis of nucleus and replacement by vacial glia. On the 7th day after the exposure local disappearance of according retinal layers was obsevred. In these local sites of the most intensive destruction in group 3 there is 1,6 times more cells with caryopyknosis from in according sites of group 2 (just light exposure) rats. Thus, alloxan diabetes on the early stage of its development (1 month) intensifies light damage of retinal photoreceptors. Probably, increase of oxydative processes and antioxidative systems break down play an important role in this synergetic effect.

scholarly journals HISTOLOGICAL STRUCTURE OF PROXIMAL METAPHYSEAL CARTILAGE OF HUMERUS IN RATS AFTER 60-DAY TARTRAZINE ADMINISTRATION

2020 ◽  
pp. 150-157
Author(s):  
V.I. Luzin ◽  
O.N. Fastova ◽  
V.N. Morozov ◽  
E.N. Morozova ◽  
S.V. Zabolotnaya
Keyword(s):  
Body Weight ◽  
Male Rats ◽  
Control Group ◽  
Histological Structure ◽  
Total Width ◽  
Adult Rats ◽  
Study Results ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

The aim of the research is to study the histological structure of proximal metaphysial cartilage of the humerus in adult rats after 60 days of tartrazine administration (750 mg/kg and 1500 mg/kg body weight). Materials and Methods. The study was carried out on 90 white male rats, divided into 3 groups. In Group 1 (control), animals daily intragastrically received 1ml of 0.9 % isotonic sodium chloride solution for 60 days. Rats of Groups 2 and Group 3 received intragastrically 1ml of tartrazine solution (750 mg/kg and 1500 mg/kg body weight respectively) for 60 days. The readaptation period after the last tartrazine administration was 3, 10, 15, 24, or 45 days. The area of the proximal humerus metaphysis underwent treatment according to the standard histological protocol. The obtained sections were stained with hematoxylin-eosin and subjected to a subsequent histomorphometric study. Results. On the 3rd day of the readaptation period, the total width of the proximal metaphysial cartilage of the humerus in animals of Group 2, was by 8.59 % less than the values of the control group; the width of the indifferent, proliferating and definitive cartilage was by 6.11 %, 8.85 % and 7.15 % less; the width of the destruction zone was by 11.55 % less; and the width of the osteogenesis zone was by 9.92 % less. The content of primary spongiosis in the osteogenesis zone and the number of cells on the trabeculae surface were lower than the values of Group 1 by 8.45 % and 8.42 % respectively. During the readaptation period, the authors observed similar dynamics of changes in the histomorphometric parameters of the metaphysical cartilage of the humerus with levelling by the 45th day of the experiment. In rats of Group 3, during the entire readaptation period, the histomorphometric parameters changed more significantly and their recovery to control values was slower than in animals of Group 2. Conclusion. 60-day tartrazine administration to adult rats is accompanied by inhibition of the bone formation function of the proximal metaphysial cartilage of the humerus. It is confirmed by a decrease in the width of all its zones and components of the osteogenesis zone. More pronounced in amplitude and duration changes in the parameters studied were recorded in animals treated with tartrazine, 1500 mg/kg body weight. Keywords: humerus, metaphysial cartilage, tartrazine, histomorphometry. Цель исследования. Установить, оказывает ли влияние 60-дневное введение тартразина в дозах 750 и 1500 мг/кг массы тела на гистологическое строение проксимального метафизарного хряща плечевой кости у половозрелых крыс. Материалы и методы. Исследование проведено на 90 белых крысах-самцах, распределенных на 3 группы: 1-я – контрольная, в которой животные ежедневно в течение 60 дней получали физиологический раствор через желудочный зонд; 2-ю и 3-ю группы составили крысы, которые на протяжении 60 дней получали внутрижелудочно 1 мл раствора тартразина в дозах 750 и 1500 мг/кг массы тела соответственно. Сроки периода реадаптации после окончания введения тартразина составляли 3, 10, 15, 24, 45 сут. Проксимальный метафиз плечевой кости подвергался обработке по стандартному гистологическому протоколу. Полученные срезы окрашивались гематоксилин-эозином и подвергались последующему гистоморфометрическому исследованию. Результаты. У животных 2-й группы на 3-й день периода реадаптации общая ширина проксимального метафизарного хряща плечевой кости была меньше значений контрольной группы на 8,59 %, ширина зон индифферентного, пролиферирующего и дефинитивного хряща – на 6,11, 8,85 и 7,15 % меньше, ширина зоны деструкции – на 11,55 %, а ширина зоны остеогенеза – на 9,92 %. Содержание первичной спонгиозы в зоне остеогенеза и количество клеток на поверхности трабекул были меньше значений контрольной группы на 8,45 и 8,42 %. В ходе периода реадаптации сходная динамика изменений гистоморфометрических параметров метафизарного хряща плечевой кости сохранялась с тенденцией к сглаживанию к 45-м сут эксперимента. У крыс 3-й группы в ходе всего периода реадаптации изучаемые параметры изменялись более значимо и их восстановление до контрольных величин происходило медленнее, чем во 2-й группе. Выводы. 60-дневное введение тартразина половозрелым крысам сопровождается угнетением костеобразовательной функции проксимального метафизарного хряща плечевой кости, что подтверждается снижением ширины всех его зон и объемных компонентов зоны остеогенеза. Более выраженные по амплитуде и длительности изменения изучаемых параметров зафиксированы у животных, получавших тартразин в дозе 1500 мг/кг массы тела. Ключевые слова: плечевая кость, метафизарный хрящ, тартразин, гистоморфометрия.

scholarly journals The Effect of Anti Masquito Electric Gas that Consist Allethrin That Influence The Weight and Colour of Rats Liver

2017 ◽  
Vol 6 (2) ◽  
pp. 6
Author(s):  
Nurmawanti Nurmawanti ◽  
Ayly Soekanto
Keyword(s):  
Male Rats ◽  
Control Group ◽  
Control Groups ◽  
Control Group Design ◽  
Significant Difference ◽  
Post Test ◽  
Group 3 ◽  
The Subject ◽  
Group 2 ◽  
Group 1

The aim of this research is to know the influence of anti mosquito electric gas that consisted Allethrin to the weight and colour of the rats liver. This research used an experimental method with the post test only  control group design.  The subject of this research is male rats weight of 150 grams each and total there was 24 rats that were divided to 4 groups. The first one, the  control groups (PO) was not given any of the gas, the second one, group 1 (P1) was given the gas for 4 hours everday, the third one, group  2 (P2), was given for 6 hours and last, group 3  (P3) was given 8 hours everyday. After later, on  30 Th day, the rats were terminated and being put in a surgery to remove their liver. This data was analyzed using SPPS for windows version 16. To see the differences in weight between the groups, it was analyzed using anova, and  to obtain the discoloration of the rats liver was analyzed using Kruskal Wallis Test. From the statistic tests, it show that there is significant difference in weight and color of liver in the group that α ≤ 0,05. According Anova Test, it shows that there is a significant difference α = 0,034 and from Kruskal Wallis test α = 0,013. In the conclusion anti mosquito electric gas that consist Allethrin affects the weight and the color of rats liver.

Intracisternal Membranes in Tumor Cells

1976 ◽  
Vol 34 ◽  
pp. 262-263
Author(s):  
E.C. Chew ◽  
C.N. Sun ◽  
H.J. White
Keyword(s):  
Endoplasmic Reticulum ◽  
Tumor Cells ◽  
Osmium Tetroxide ◽  
Hepatoma Cells ◽  
Uranyl Acetate ◽  
Granular Endoplasmic Reticulum ◽  
Lead Citrate ◽  
Routine Procedures ◽  
Course Of Study

To our knowledge, membranous structures have not been heretofore reported in the cisternae of granular endoplasmic reticulum. Such unusual structures were seen in the course of study of Chang's hepatoma cells (1).The tumor cells from Chang's hepatoma were kindly given us by Professor J.P. Chang and carried in our laboratory by subcutaneous transplantations. Tissue fragments were fixed in 4% phosphate buffered glutarladehyde and post-fixed in 1% osmium tetroxide. Routine procedures were followed for dehydration and embedding in Epon 812. Sections were stained with uranyl acetate and lead citrate.As cited above, cisternal membranous structures were observed (Fig. 1), and similar structures were seen in the perinuclear cisternae (Figs. 2&3). The latter were devoid of ribosomes. In some instances, these membranes were not connected to the original membranes of the granular endoplasmic reticulum, while in many cases they were fused with the pre-existing membranes forming two separate cisternae (Figs. 1,2&3). These structures were also seen in hepatoma cells undergoing mitosis (Fig. 4).

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