OINTMENT OF Eupatorium odoratum L. EXTRACT PROMOTES BURN WOUND HEALING IN MALE ALBINO MICE
Eupatorium odoratum L. is traditionally used to treat open wounds. Aim of this study was toinvestigate the healing activity of ointment containing extract of E. odoratum in burns induced in malealbino mice. Mice were divided into 5 groups, all were induced for burn wound using a heat stamp intemperature 80°C for 20 minutes. Group I was treated with ointment base (control), group II, III and IVwere treated with extract ointment in concentration of 5%, 10%, and 20% w/w respectively, and the lastgroup V was treated with ointment reference. Observations were made during 21 days exactly on the 7th,14th and 21st day, which included parameters: percentage of healed area, epithelialization time andcollagen scores. The result showed that on 14th day, mean of healed area in group III (75.89%±7.76%)and IV (76.29%±6.981%) were significantly higher than other groups (P<0.05). On the 14th and 21th day,collagen scores in groups III (2.33±0.577 and 2.67±0.577) and IV (2.67±0.577 and 2.33±0.577) are equalwith reference group, these score are significantly higher than in groups I and II (P<0.05). Meanwhile,though epithelialization time in group III and IV (both 14.33 days±0.577days) are more quickly thanothers, statistical analysis showed that there is no significant difference on time between all groups(P>0.05).