Skrining Fitokimia dan Uji Bioaktivitas Antibakteri dari Gorgonia Mopsella sp. *cf, Siphonogorgia sp. dan Villogorgia sp. terhadap Bakteri Pseudomonas aeruginosa dan Bacillus cereus (Phytochemical screening test the antibacterial bioactivity of gorgonia Mopsella sp. *cf, Siphonogorgia sp. and Villogorgia sp. Againts Pseudomonas aeruginosa and Bacillus cereus)

2019 ◽  
Vol 9 (2) ◽  
pp. 83
Author(s):  
Ni Made Marlin Suarjo Putri ◽  
Febby Kandou ◽  
Marina Singkoh
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Bacillus Cereus ◽  
Screening Test ◽  
Ethanol Extract ◽  
Test Results ◽  
Phytochemical Screening ◽  
Inhibitory Power ◽  
E Mail

Skrining Fitokimia dan Uji Bioaktivitas Antibakteri dari Gorgonia Mopsella sp. *cf, Siphonogorgia sp. dan Villogorgia sp. terhadap Bakteri Pseudomonas aeruginosa dan Bacillus cereus(Phytochemical screening test the antibacterial bioactivity of gorgonia Mopsella sp. *cf, Siphonogorgia sp. and Villogorgia sp. Againts Pseudomonas aeruginosa and Bacillus cereus) Ni Made Marlin Suarjo Putri1*), Febby Ester Fany Kandou1), Marina Singkoh1)1)Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan, Universitas Sam Ratulangi Manado*E-mail: [email protected] Diterima 7 Juli 2019, diterima untuk dipublikasi 10 Agustus 2019 ABSTRAK Penelitian ini bertujuan untuk menguji bioaktivitas antibakteri dari ekstrak etanol Mopsella sp. *cf, Siphonogorgia sp. dan Villogorgia sp. terhadap Pseudomonas aeruginosa dan Bacillus cereus dan mengetahui golongan senyawa yang terkandung di dalamnya. Tahapan penelitian meliputi pengambilan dan penyiapan sampel, identifikasi Gorgonia, ekstraksi, skrining fitokimia, pembuatan larutan Mc. Farland, pembuatan media dan pengujian antibakteri. Ekstraksi dilakukan dengan cara maserasi menggunakan pelarut etanol. Pengujian bioaktivitas antibakteri menggunakan metode Kirby- Bauer. Hasil uji bioaktivitas antibakteri menunjukkan ekstrak etanol Mopsella sp. memiliki aktivitas antibakteri dengan kategori kuat (13,5 mm ± 2,60), Siphonogorgia sp. termasuk dalam kategori sedang (9 mm ± 1,80), Villogorgia sp. tidak memiliki daya hambat (6,5 mm ± 3,46) pada bakteri Pseudomonas aeruginosa. Sedangkan pada bakteri uji Bacillus cereus, pada sampel Mopsella sp tidak memiliki daya hambat, sampel Siphonogorgia sp. termasuk dalam kategori sedang (8,3 mm ± 0,58), Villogorgia sp. tidak memiliki daya hambat (6,5 mm ± 5,07).  Golongan senyawa yang terkandung dalam Mopsella sp. yaitu flavonoid, saponin, tanin. Golongan senyawa pada Siphonogorgia sp. yaitu alkaloid (Wagner) menunjukkan positif sedangkan golongan senyawa pada Villogorgia sp. yaitu alkaloid dan flavonoid.  Kata kunci:      skrining fitokimia, antibakteri, Mopsella sp., Siphonogorgia sp., Villogorgia sp. ABSTRACT This study aims to examine the antibacterial bioactivity of ethanol extract of Mopsella sp. * cf, Siphonogorgia sp. and Villogorgia sp. against Pseudomonas aeruginosa and Bacillus cereus and find out the class of compounds contained therein. The stages of the research included sampling and preparation, Gorgonia identification, extraction, phytochemical screening, making Mc solution. Farland, making media and antibacterial testing. Extraction was carried out by maceration using ethanol solvent. Antibacterial bioactivity testing using the Kirby-Bauer method. Antibacterial bioactivity test results showed that ethanol extract of Mopsella sp. had antibacterial activity with a strong category (13.5 mm ± 2.60), Siphonogorgia sp. included in the medium category (9 mm ± 1.80), Villogorgia sp. had no inhibitory power (6.5 mm ± 3.46) in the Pseudomonas aeruginosa bacteria. Whereas in Bacillus cereus test bacteria, in Mopsella sp. sample did not have inhibitory power, Siphonogorgia sp. sample was included in the medium category (8.3 mm ± 0.58), Villogorgia sp. did not have inhibitory power (6.5 mm ± 5.07). The groups of compounds contained in Mopsella sp. are flavonoids, saponins, tannins. The group of compounds in Siphonogorgia sp., namely alkaloids (Wagner) showed positivity while the compound groups in Villogorgia sp. were alkaloids and flavonoids.Key words: Phytochemical screening, Antibacterial, Mopsella sp., Siphonogorgia sp., Villogorgia sp.

scholarly journals Skrining Fitokimia dan Uji Bioaktivitas Antibakteri dari Gorgonia Mopsella sp. *cf, Siphonogorgia sp. dan Villogorgia sp. terhadap Bakteri Pseudomonas aeruginosa dan Bacillus cereus (Phytochemical screening test the antibacterial bioactivity of gorgonia Mopsella sp. *cf, Siphonogorgia sp. and Villogorgia sp. Againts Pseudomonas aeruginosa and Bacillus cereus)

2019 ◽  
Vol 9 (2) ◽  
pp. 83
Author(s):  
Ni Made Marlin Suarjo Putri ◽  
Febby Ester Fany Kandou ◽  
Marina Singkoh
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Bacillus Cereus ◽  
Screening Test ◽  
Ethanol Extract ◽  
Test Results ◽  
Phytochemical Screening ◽  
Inhibitory Power ◽  
E Mail

Skrining Fitokimia dan Uji Bioaktivitas Antibakteri dari Gorgonia Mopsella sp. *cf, Siphonogorgia sp. dan Villogorgia sp. terhadap Bakteri Pseudomonas aeruginosa dan Bacillus cereus(Phytochemical screening test the antibacterial bioactivity of gorgonia Mopsella sp. *cf, Siphonogorgia sp. and Villogorgia sp. Againts Pseudomonas aeruginosa and Bacillus cereus) Ni Made Marlin Suarjo Putri1*), Febby Ester Fany Kandou1), Marina Singkoh1)1)Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan, Universitas Sam Ratulangi Manado*E-mail: [email protected] Diterima 7 Juli 2019, diterima untuk dipublikasi 10 Agustus 2019 ABSTRAK Penelitian ini bertujuan untuk menguji bioaktivitas antibakteri dari ekstrak etanol Mopsella sp. *cf, Siphonogorgia sp. dan Villogorgia sp. terhadap Pseudomonas aeruginosa dan Bacillus cereus dan mengetahui golongan senyawa yang terkandung di dalamnya. Tahapan penelitian meliputi pengambilan dan penyiapan sampel, identifikasi Gorgonia, ekstraksi, skrining fitokimia, pembuatan larutan Mc. Farland, pembuatan media dan pengujian antibakteri. Ekstraksi dilakukan dengan cara maserasi menggunakan pelarut etanol. Pengujian bioaktivitas antibakteri menggunakan metode Kirby- Bauer. Hasil uji bioaktivitas antibakteri menunjukkan ekstrak etanol Mopsella sp. memiliki aktivitas antibakteri dengan kategori kuat (13,5 mm ± 2,60), Siphonogorgia sp. termasuk dalam kategori sedang (9 mm ± 1,80), Villogorgia sp. tidak memiliki daya hambat (6,5 mm ± 0,87) pada bakteri Pseudomonas aeruginosa. Sedangkan pada bakteri uji Bacillus cereus, pada sampel Mopsella sp tidak memiliki daya hambat, sampel Siphonogorgia sp. termasuk dalam kategori sedang (8,3 mm ± 0,58), Villogorgia sp. tidak memiliki daya hambat (6,5 mm ± 0,50).  Golongan senyawa yang terkandung dalam Mopsella sp. yaitu flavonoid, saponin, tanin. Golongan senyawa pada Siphonogorgia sp. yaitu alkaloid (Wagner) menunjukkan positif sedangkan golongan senyawa pada Villogorgia sp. yaitu alkaloid dan flavonoid.  Kata kunci:      skrining fitokimia, antibakteri, Mopsella sp., Siphonogorgia sp., Villogorgia sp. ABSTRACT This study aims to examine the antibacterial bioactivity of ethanol extract of Mopsella sp. * cf, Siphonogorgia sp. and Villogorgia sp. against Pseudomonas aeruginosa and Bacillus cereus and find out the class of compounds contained therein. The stages of the research included sampling and preparation, Gorgonia identification, extraction, phytochemical screening, making Mc solution. Farland, making media and antibacterial testing. Extraction was carried out by maceration using ethanol solvent. Antibacterial bioactivity testing using the Kirby-Bauer method. Antibacterial bioactivity test results showed that ethanol extract of Mopsella sp. had antibacterial activity with a strong category (13.5 mm ± 2.60), Siphonogorgia sp. included in the medium category (9 mm ± 1.80), Villogorgia sp. had no inhibitory power (6.5 mm ± 0,87) in the Pseudomonas aeruginosa bacteria. Whereas in Bacillus cereus test bacteria, in Mopsella sp. sample did not have inhibitory power, Siphonogorgia sp. sample was included in the medium category (8.3 mm ± 0.58), Villogorgia sp. did not have inhibitory power (6.5 mm ± 0,50). The groups of compounds contained in Mopsella sp. are flavonoids, saponins, tannins. The group of compounds in Siphonogorgia sp., namely alkaloids (Wagner) showed positivity while the compound groups in Villogorgia sp. were alkaloids and flavonoids.Key words: Phytochemical screening, Antibacterial, Mopsella sp., Siphonogorgia sp., Villogorgia sp.

scholarly journals Antibacterial Activity Test of Ethanol Extract from Dried Simplicia Garlic (Allium sativum L.) Against Bacillus cereus Bacteria

2020 ◽  
Vol 3 (2) ◽  
pp. 93-99
Author(s):  
Athaillah Athaillah ◽  
Ugi Diana Lestari
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Cereus ◽  
Allium Sativum ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Disk Diffusion Method ◽  
Activity Test ◽  
Inhibitory Power ◽  
Dried Extract

This research is aimed to find out the extract activity dried simplisia of garlic (Allium sativum L.) as an antibacterial which is capable to inhibiting growth of Bacillus cereus bacterium, to find out the alkaloid secondary of metabolites, flavonoid, tannin and saponin that contained in dried extract of garlic and to find out a concentration that has the most antibacterial activity which is capable to inhibiting growth of Bacillus cereus bacterium. Garlic was extracted by maceration method using a solvent ethanol 96%. After the extract obtaned, then phtochemical screening and standardization test. Thickening technique by evaporation used vacum rotary evaporator until thick extarct was obtained. Antibacterial activity thest by using disk diffusion method. This research was used seven concentrations which were 20% (b/v), 30% (b/v). 40% (b/v), 50% (b/v), 60% (b/v), 80% (b/v)and100% (b/v). The result of phytochemical screening test, ethanol extract garlic (Allium sativum L.) positive contained compound alkaloid, flavonoid, saponin and tannin. Standardization of power simplisia fulfill the requirements set by Depkes RI 2000. The result of activity test of optimum antibacterial was obtained inhibitory power with the number 27 mm on 40% (b/v) concentration. This proves that garlic (Allium sativum L.) have antibacterial affects against Bacillus cereus bacterium

scholarly journals Antibacterial Activity of Combination of Ethanol Extract of Pepermine Leaves (Mentha piperita L.) and Amikacin Against Klebsiella pneumonia, Staphylococcus aureus, and Escherichia coli

2021 ◽  
Vol 4 (1) ◽  
pp. 12-29
Author(s):  
Peni Indrayudha
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Screening Test ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Mentha Piperita ◽  
Klebsiella Pneumonia ◽  
Phytochemical Screening

Peppermint leaves have been known to have antibacterial and antifungal activity. Amikacin is a semisynthetic derivative of kanamycin which is active against both Gram-positive and Gram-negative enteric bacteria. The combination of plant extracts with antibiotics is one way or alternative to overcome bacterial resistance to antibiotics. The purpose of this study was to determine the combined effect of the ethanolic extract of peppermint (Mentha piperita L.) and amikacin against Klebsiella pneumonia, Staphylococcus aureus, and Escherichia coli as well as the compounds contained in the ethanolic extract of peppermint (Mentha piperita L.) leaves. Antibacterial activity was tested using the disk diffusion method (Kirby Bauer) and the phytochemical screening test using the tube test method. The concentration of peppermint leaf ethanol extract for the combination test was 200 mg/mL and 400 mg/mL, for the concentration of amikacin used was 5 mg/mL with three comparisons made, namely 25:75, 50:50, and 75:25. The results showed a synergistic effect with the largest inhibition zone diameter at a ratio of 25:75 at a concentration of 200 mg/mL, which was 36.25±2.5 mm on Klebsiella pneumonia bacteria. While at a concentration of 400 mg/mL the diameter of the largest inhibition zone was 40±1.63 mm in Klebsiella pneumoniae bacteria with a ratio of 75:25. The results of statistical tests using the t-test showed a significance value of 0.000 0.05 so that there was a significant difference in the administration of each concentration to the resulting inhibition zone. The results of the phytochemical screening test contained alkaloids, phenolic compounds, flavonoids, tannins.

scholarly journals Antibacterial activity of the sambiloto leaf extracts (Andrographis paniculata) to Bacillus cereus and Pseudomonas aeruginosa

2017 ◽  
Vol 14 (1) ◽  
pp. 19-24
Author(s):  
RUTH NOVA MARDIANA ◽  
NESTRI HANDAYANI
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Bacillus Cereus ◽  
Leaf Extract ◽  
Inhibitory Concentration ◽  
Ethanol Extract ◽  
Andrographis Paniculata ◽  
Diffusion Method ◽  
Equivalence Point ◽  
Leaf Extracts

Mardiana RN, Handayani N. 2016. Antibacterial activity of the sambiloto leaf extracts (Andrographis paniculata) to Bacillus cereus and Pseudomonas aeruginosa. Biofarmasi 14: 19-24. The purpose of this research was to evaluate the antibacterial activity of sambiloto ( Andrographis paniculata Nees. ) extract against bacterial Bacillus cereus and Pseudomonas aeruginosa. Some research had proven sambiloto leaf extract evident had antibacterial activity to bacteria Staphylococcus aureus. The sample of sambiloto leaf was macerated using 70% ethanol, and the extract was concentrated using rotary evaporator. The antibacterial activity was evaluated by diffusion method. Antibacterial potency of the extract was compared with by Amoxicillin made default curve among logarithm concentrates to constraining diameter (mm), then accounted by its equivalence point. Ethanol extract of sambiloto leaf had antibacterial activity to B. cereus and P. aeruginosa. Minimum Inhibitory Concentration (MIC) of 12,5% against B. cereus and P. aeruginosa. The potential antibacterial activity of sambiloto leaf extract was 0,2% for B. cereus and 0.3% for P. aeruginosa; as compared with Amoxicillin. This, potential antibacterial activity of sambiloto leaf extract to the two tested bacteria was much smaller than that of Amoxicillin synthetic antibiotic.

scholarly journals Antibacterial Activity Test of Ethanol Extract and SAP of Betung Bamboo Shoot (Dendrocalamus asper) Against Klebsiella pneumoniae and Pseudomonas aeruginosa Bacteria

2021 ◽  
Vol 1 (1) ◽  
pp. 11
Author(s):  
Anif Nur Artanti ◽  
Farikatul Sufi Mujahidah
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Klebsiella Pneumoniae ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Bamboo Shoot ◽  
Activity Test ◽  
Bamboo Shoots ◽  
Dendrocalamus Asper ◽  
Inhibitory Power

<p>Boiled water from bamboo shoots is often used for pneumonia and a cleanser for the wound. It is known that bamboo can be an antibacterial agent because it has saponin, alkaloid, and flavonoid. This research aims to study whether ethanol extract and sap from bamboo betung shoot (<em>Dendrocalamus asper</em>) has secondary metabolism and antibacterial activity against <em>Klebsiella pneumoniae</em> and <em>Pseudomonas aeruginosa</em>. The extract was taken by maceration method using ethanol 96%, and the sap was taken from the water of bamboo shoot. Antibacterial activity test was conducted using disc diffusion method to measure the magnitude of inhibitory power at six concentration ratios of b/v solutions (10%; 20%; 30%; 40%; 50%; 60%). The result showed that ethanol extract and sap of bamboo betung shoot contained saponin, quercetin, and quinine which were supposed to have antibacterial activity against <em>Klebsiella pneumoniae</em> and <em>Pseudomonas aeruginosa</em>. The largest inhibitory zone diameter was found in ethanol extract 60% with the category of moderate inhibition of 9.05 ± 0.12 mm against <em>Kliebsiella pneumoniae</em> and 5.07 ± 0.13 mm against <em>Pseudomonas aeruginosa</em>. While the sap with a concentration of 60% was included in the weak inhibitory category, which was 5.65 ± 0.05 mm in the <em>Kliebsiella pneumoniae</em> and 4.81 ± 0.22 mm in <em>Pseudomonas aeruginosa</em>.<strong></strong></p><p> </p>Keywords: antibacterial; bamboo shoot; betung bamboo.

scholarly journals Antibacterial Activity of Leaf Extracts ofBaeckea frutescensagainst Methicillin-ResistantStaphylococcus aureus

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Somayeh Razmavar ◽  
Mahmood Ameen Abdulla ◽  
Salmah Binti Ismail ◽  
Pouya Hassandarvish
Keyword(s):  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Phytochemical Screening ◽  
Bioactive Constituents ◽  
Disc Diffusion Method ◽  
Leaf Extracts ◽  
Baeckea Frutescens ◽  
High Degree

This study was based on screening antibacterial activity of the ethanol extract ofBaeckea frutescensL. against MRSA clinical isolates, analyzes the potential antibacterial compound, and assesses the cytotoxicity effect of the extract in tissue culture. Leaves ofBaeckea frutescensL. were shade dried, powdered, and extracted using solvent ethanol. Preliminary phytochemical screening of the crude extracts revealed the presence of alkaloids, flavonoids, steroids, terpenoids, phenols, and carbohydrates. The presence of these bioactive constituents is related to the antibacterial activity of the plant. Disc diffusion method revealed a high degree of activity against microorganisms. The results confirm thatBaeckea frutescensL. can be used as a source of drugs to fight infections caused by susceptible bacteria.

scholarly journals Preliminary phytochemical screening and susceptibility of bacteria pathogens to whole extract of evolvulus alsinoides (L.)

1970 ◽  
Vol 18 ◽  
pp. 16-20
Author(s):  
BA Omogbai ◽  
FA Eze
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Bacillus Cereus ◽  
Pathogenic Bacteria ◽  
Volatile Oil ◽  
Salmonella Typhi ◽  
Phytochemical Screening ◽  
Evolvulus Alsinoides ◽  
And Staphylococcus Aureus

Context: Plant based antimicrobial represent a vast untapped source for medicines and further exploration of plant antimicrobial neeto occur. Evolvulus alsinoides (L) (Convolvulaceae) is a perennial herb is used in traditional medicine in East Asia, India, Africa and Philippines to cure fever, cough, cold, venereal diseases, azoospermia, adenitis and dementia.   Objective: The objective of this research was to evaluate the antimicrobial activity of the extracts of E. alsinoides on some clinical microbial isolates.   Materials and Methods: The ed thanolic and aqueous extracts of the whole plant (leaves and twigs) were analysed for alkanoids, tannins, glycosides, steroids, flavonoids, saponins, volatile oil and resins. The determination of antibacterial activity was done using the agar well diffusion technique. Pure cultures of pathogenic bacteria such as Bacillus cereus, Staphylococcus aureus, Micrococcus leutus, Klebsiella Pneumoniae, Pseudomonas aeruginosa, Escherichia coli and Salmonella typhi were used for antibacterial activity assay, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC).   Results: The ethanolic extract of the plant had MIC values ranging from 16 mg/ml to 512.5 mg/ml. The least MIC was 16mg-ml against Salmonella typhi while Bacillus cereus and Staphylococcus aureus showed the highest MIC of 512.5 mg-ml. In the aqueous extract the MIC ranged between 512.5 to >1025 mg/ml. Salmonella typhi, Micrococcus luteus and Staphylococcus aureus were not inhibited by the water extract. Phytochemical result showed ethanol to be a better solvent for the extraction of the bioactive agents in this plant which include: glycosides, alkaloids, saponins, tannins, flavonoids and volatile oil.   Conclusion: In this study the gram-negative organisms had the lowest MICs and MBCs. This suggests their higher susceptibility to the extract of this plant. On the basis of the result obtained in this investigation it can be concluded that ethanol extract of Evolvulus alsinoides had significant in vitro broad spectrum antimicrobial activity.   Keywords: Evolvulus alsinoides; Phytochemical screening; Antibacterial activity. DOI: http://dx.doi.org/10.3329/jbs.v18i0.8769 JBS 2010; 18(0): 16-20

scholarly journals Uji Aktivitas Antibakteri dari Ekstrak dan Fraksi Daging Buah Mangga Kasturi (Mangifera Casturi) terhadap Bakteri Pseudomonas Aeruginosa dan Bacillus Cereus

2021 ◽  
Vol 49 (2) ◽  
pp. 113-122
Author(s):  
Meliana Meliana ◽  
Sogandi Sogandi ◽  
Ekajayanti Kining
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Bacillus Cereus ◽  
Inhibitory Concentration ◽  
Mangifera Indica ◽  
Ethyl Acetate Fraction ◽  
Gas Chromatography Mass Spectrometry

Kasturi (Mangifera casturi) is a typical plant of South Kalimantan which has only been used for consumption because of its distinctive taste and aroma. Mangifera indica, which is in the same genus as Mangifera casturi, shows antioxidant, antibacterial, antifungal, and antidiarrheal activity, so it is suspected that this plant has the same activity. Crude methanol extract from the meat of Casturi mango was fractionated using n-hexane, ethyl acetate, and methanol-water. Testing for antibacterial activity and minimum inhibitory concentration (MIC) were carried out using the disk diffusion method (Kirby-Bauer). The positive control used was Chloramphenicol because it has an antibacterial range that is rather broad and the negative control used was 10% DMSO. The fraction that had the greatest antibacterial activity was the ethyl acetate fraction; with the formation of a clear zone of 7.98mm against Pseudomonas aeruginosa and 7.52mm against Bacillus cereus, with a minimum inhibitory concentration of 2mg/mL. The next test was a cell leakage analysis which revealed an increase in absorbance at MIC 1 and MIC 2. The Gas Chromatography – Mass Spectrometry (GC-MS) results from the ethyl acetate fraction revealed the presence of phenolic, flavonoid, and glycoide content, with the highest compound concentration of 5-Hydroxymethylfurfural. Keywords: Antibacterial, Extract, Ethyl acetate, fraction, Mangifera casturi Abstrak Kasturi (Mangifera casturi) merupakan tumbuhan khas Kalimantan Selatan yang selama ini hanya dimanfaatkan untuk dikonsumsi karena rasa dan aromanya yang khas. Mangifera indica yang masih satu genus dengan Mangifera casturi menunjukkan aktivitas antioksidan, antibakteri, antijamur, dan antidiare sehingga diduga tanaman ini memiliki aktivitas yang sama. Ekstrak kasar metanol daging buah mangga kasturi difraksinasi dengan menggunakan n-heksan, etil asetat, dan metanol-air. Pengujian aktivitas antibakteri dan kadar hambat minimum dilakukan dengan menggunakan metode difusi cakram (Kirby- Bauer). Kontrol positif yang digunakan adalah Chloramfenikol karena memiliki spektrum antibakteri yang cukup luas dan kontrol negatif yang digunakan adalah DMSO 10%. Fraksi yang memiliki aktivitas antibakteri terbesar pada penelitian ini adalah fraksi etil asetat dengan terbentuknya zona bening sebesar 7,98mm terhadap Pseudomonas aeruginosa dan 7,52mm terhadap Bacillus cereus dengan kadar hambat minimum 2mg/mL. Uji selanjutnya adalah uji kebocoran sel yang menyatakan adanya peningkatan absorbansi pada KHM 1 dan KHM 2. Hasil pemeriksaan Gas Chromatography – Mass Spectrometry (GCMS) dari fraksi etil asetat menyatakan adanya kandungan fenolik, flavonoid, dan glikosida dengan senyawa tertinggi yaitu 5-Hydroxymethylfurfural. Kata kunci: Antibakteri, Ekstrak, Etil asetat, Fraksi, Mangifera castur

scholarly journals Antibacterial Activity of Ethanol Extract of Spirulina platensis

2021 ◽  
Vol 7 (2) ◽  
pp. 47-51
Author(s):  
Prayoga Pannindrya ◽  
Mega Safithri ◽  
Kustiariyah Tarman
Keyword(s):  
Antibacterial Activity ◽  
Spirulina Platensis ◽  
Ethanol Extract ◽  
Positive Control ◽  
Diffusion Method ◽  
Test Results ◽  
Nutritional Content ◽  
Disc Diffusion Method ◽  
Antibacterial Test ◽  
E Coli

Spirulina is a microalgae that has been widely used as various supplements and medicines because of its high nutritional content. The need for new antibacterial sources to solve the problem of antibiotic resistance, makes the potential of Spirulina as an antibacterial agent necessary to be researched. This study aims to analyze the antibacterial activity of ethanol extract of Spirulina platensis obtained from the Indonesian region against Staphylococcus aureus and Escherichia coli. Spirulina samples were macerated in ethanol solvent in a ratio of 1:10 (w / v). The antibacterial test used was the disc diffusion method with clindamycin positive control. Antibacterial test results showed that S. platensis in this study did not have antibacterial activity against E. coli and S. aureus.

UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL 96% DAUN MIMBA (Azadirachta indica A. Juss. ) DENGAN METODE EKSTRAKSI PERKOLASI TERHADAP PERTUMBUHAN BAKTERI

2020 ◽  
Vol 2 (1) ◽  
pp. 102
Author(s):  
Yanu Andhiarto ◽  
Rina Andayani ◽  
Nur Hidayatul Ilmiyah
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Skin Inflammation ◽  
Screening Tests ◽  
Diffusion Method ◽  
Moderate Activity ◽  
Phytochemical Screening ◽  
Strong Activity ◽  
Activity Test

Skin infections most often found in developing countries, especially in the tropics, are purulent skin inflammation (pyoderma), the main cause of Staphylococcus aureus. The increasing number of cases of bacteria that are resistant to antibacterial can encourage the extraction of sources of antibacterial drugs from natural ingredients. One of the plants that is thought to have the antibacterial activity of Staphylococcus aureus is the Neem plant. In this study an antibacterial activity test was carried out with the well diffusion method from 96% ethanol extract of Mimba leaves extracted by percolation method. Previously 96% ethanol extract from Mimba leaves had been identified by secondary metabolites by phytochemical screening and standardized extract testing. Standarization using non-specific parameters (moisture content, ash content and drying losses) found that 96% ethanol extract of Mimba leaves entered a range of standard parameters and in phytochemical screening tests obtained 96% ethanol extract of Mimba leaves containing alcaloid, saponins, tannins, steroids / terpenoids and flavonoids. The results of the study on the antibacterial activity test showed a concentration of 75% ethanol extract of 96% Mimba leaves classified as having strong activity, at a concentration of 50% and 25% classified as having moderate activity. Based on this study it can be concluded that the three concentrations of 96% ethanol extract of Mimba leaves were able to inhibit the growth of Staphylococcus aureus bacteria.

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