scholarly journals Biotherapeutic potential of dairy beverage supplemented with lactobacillus casei shirota for the control of canine hookworm

2018 ◽  
Vol 1 (1) ◽  
pp. 10
Author(s):  
Matheus Diniz Gonçalves Coêlho ◽  
Lívia Simões Silva ◽  
Lucas Tobias Rodrigues Maciel ◽  
Carolina Laurindo Moreira ◽  
Taís Cristina Paschoal ◽  
...  
Keyword(s):  
Protective Effect ◽  
Lactobacillus Casei ◽  
Alternative Therapy ◽  
Control Group ◽  
Lactobacillus Casei Shirota ◽  
Natural Hosts ◽  
Parasitic Load ◽  
Healthy Dogs ◽  
Probiotic Preparation ◽  
Potential Use

Domestic dogs (Canis familiaris) are present in the homes of several families around the world, and are one of the animals with greater contact with humans, either as a guard dog or just as a pet. With this, they can transmit parasites to their owners, since dogs are natural hosts of several parasites, among them the helminths of Ancylostomidae family. Ancylostomiasis presents as main symptoms abdominal pain and anemia. The treatment is performed with anthelmintics, however, it was found that these parasites are acquiring resistance to current drugs, which makes necessary the development of alternative therapies. Thus, this study aims to evaluate the therapeutic effect of a probiotic preparation containing 1x1010 CFU of Lactobacillus casei Shirota, administering 80 mL of this, for a group of four naturally infected dogs during 40 alternate days and, in parallel, administering a same volume during 30 consecutive days for a similar group of dogs. The protective effect was also evaluated by administering 80 ml of the preparation for a group of 5 healthy dogs for 30 consecutive days, which were maintained along with other infected animals. The number of eggs per gram of feces (EPG) was determined every 7 days, noting possible changes. As results, it was observed the potential use of probiotics in the treatment of hookworm in dogs as an inexpensive alternative therapy, since the assessed preparation induced significant reduction of the parasitic load of infected animals (p <0.005) in comparison with the control group, but in the other hand it was not observed protective effect, since the parasitosis have established in the dogs which were pretreated with L. casei Shirota.

scholarly journals THE EFFECT OF SUPPLEMENTATION PROBIOTIC Lactobacillus casei STRAIN Shirota L AGAINST COLONIZATION OF Staphylococcus aureus Nasal Swab ISOLATES FROM PATIENTS WITH ALLERGIC RHINITIS

2015 ◽  
Vol 2 (1) ◽  
pp. 124
Author(s):  
Lilis Suryani
Keyword(s):  
Staphylococcus Aureus ◽  
Allergic Rhinitis ◽  
Lactobacillus Casei ◽  
Nasal Swab ◽  
Intestinal Flora ◽  
Control Group ◽  
School Of Medicine ◽  
Lactobacillus Casei Shirota ◽  
Probiotic Lactobacillus ◽  
Quasi Experimental

<p>Staphylococcus aureus is the most important type of bacteria causing infections in humans. There are several potential factors for Staphylococcus aureus nasal careers in healthy adults, i.e. chronic sinusitis, the long-term skin trauma, respiratory allergies, asthma, allergen injection therapy. Probiotics are life microorganisms which have a positive effect on prevention or treatment of certain disease conditions when consumed. Manipulation of intestinal flora with probiotics for the prevention and treatment of rhinitis allergy should be developed. This study aims to determine the effect of supplementation probiotic Lactobacillus casei Shirota strain against colonization Staphylococcus aureus nasal swab isolates from patients with allergic rhinitis. <br />This research was quasi-experimental case-control design with a control group pretest-postest design. The study was conducted at the Microbiology and Research Laboratory School of Medicine Muhammadiyah Yogyakarta University, on February-July 2011. Fourty two students school of medicine Muhammadiyah Yogyakarta University, with the inclusion criteria suspect allergic rhinitis based on history. Volunteers who have a history of allergic rhinitis were taken nasal swab to check the number of S.aureus bacteria. Volunters consumed milk containing Lactobacillus casei strain Shirota daily for a month, then taken to be examined nasal swab S.aureus bacteria numbers. <br />The results showed that the average number of bacteria nasal swab isolates before supplementation about 92 CFU. After given the probiotic Lactobacillus casei Shirota strain during a month, the average number of S.aureus about 27 CFU. It can be concluded that supplementation with Lactobacillus casei strain Shirota probiotics can reduce Staphylococcus aureus colonization in patients with allergic rhinitis.</p><p><br /><strong>Keywords</strong>: Staphylococcus aureus, Lactobacillus casei, allergic rhinitis</p>

scholarly journals EFFECT OF PROBIOTIC AND PREBIOTIC CONSUMPTION ON BLOOD CHOLESTEROL LEVEL IN THE ELDERLY IN POSYANDU MANISJANGAN YOGYAKARTA

2021 ◽  
Vol 10 (3) ◽  
pp. 227-232
Author(s):  
Diyan Yunanto Setyaji ◽  
Fransisca Anjar Rina Setyani
Keyword(s):  
Cholesterol Level ◽  
Mung Bean ◽  
Lactobacillus Casei ◽  
The Elderly ◽  
Fermented Milk ◽  
Blood Cholesterol ◽  
Control Group ◽  
Blood Cholesterol Level ◽  
Cholesterol Levels ◽  
Lactobacillus Casei Shirota

Background: With advanced age, an increase in lipid profile is associated with the incidence of cardiovascular disease. Previous studies showed that the administration of probiotic and/or prebiotic are effective in improving lipid profiles. However, other studies have also shown that probiotic and prebiotic had insignificant effects on lipid profiles.Objectives: Determined the effects of probiotic and prebiotic on blood cholesterol level in the elderly.Methods: A total of 64 elderly who did not take cholesterol-lowering drugs enrolled in the quasi-experiment pretest-posttest design and were assigned to three interventions and control groups. The subjects in PRE-group consumed 100 grams of mung bean porridge per day; the subjects in PRO-group consumed 65 ml of fermented milk enriched with Lactobacillus casei Shirota strain per day; the subjects in PREPRO-group consumed 100 grams of mung bean porridge and 65 ml of fermented milk enriched with Lactobacillus casei Shirota strain per day. All intervention groups received treatment for 4 weeks. The control group did not receive any treatment during the study. Before and after treatment, cholesterol data was collected. Paired t-test, ANOVA, Post-hoc LSD were used for statistical analysis.Results There was an increase in cholesterol levels in PRE, PRO, PREPRO-groups (12.93 mg/dL; 48.37 mg/dL; and 7.62 mg/dL). Among the three intervention groups, only the consumption of probiotic was significant for changes in blood cholesterol levels (p 0.023). The administration of various interventions did not have a significant effect on changes in cholesterol levels (p 0.246) when compared to the control group.Conclusion: Prebiotic and probiotic do not lower blood cholesterol level in the elderly in Posyandu Manisjangan Yogyakarta.

scholarly journals Gut Microbiota Profiling of Aflatoxin B1-Induced Rats Treated with Lactobacillus casei Shirota

Toxins ◽  
2019 ◽  
Vol 11 (1) ◽  
pp. 49 ◽  
Author(s):  
Winnie-Pui-Pui Liew ◽  
Sabran Mohd-Redzwan ◽  
Leslie Than
Keyword(s):  
Gut Microbiota ◽  
Aflatoxin B1 ◽  
Lactobacillus Casei ◽  
Diversity Index ◽  
Alpha Diversity ◽  
Diversity Indices ◽  
Control Group ◽  
Lactobacillus Casei Shirota ◽  
Pcr Products ◽  
Miseq Platform

Aflatoxin B1 (AFB1) is a ubiquitous carcinogenic food contaminant. Gut microbiota is of vital importance for the host’s health, regrettably, limited studies have reported the effects of xenobiotic toxins towards gut microbiota. Thus, the present study aims to investigate the interactions between AFB1 and the gut microbiota. Besides, an AFB1-binding microorganism, Lactobacillus casei Shirota (Lcs) was tested on its ability to ameliorate the changes on gut microbiota induced by AFB1. The fecal contents of three groups of rats included an untreated control group, an AFB1 group, as well as an Lcs + AFB1 group, were analyzed. Using the MiSeq platform, the PCR products of 16S rDNA gene extracted from the feces were subjected to next-generation sequencing. The alpha diversity index (Shannon) showed that the richness of communities increased significantly in the Lcs + AFB1 group compared to the control and AFB1 groups. Meanwhile, beta diversity indices demonstrated that AFB1 group significantly deviated from the control and Lcs + AFB1 groups. AFB1-exposed rats were especially high in Alloprevotella spp. abundance. Such alteration in the bacterial composition might give an insight on the interactions of AFB1 towards gut microbiota and how Lcs plays its role in detoxification of AFB1.

scholarly journals The differences in the number of fibroblasts and blood vessels after the topical and systemic administration of Lactobacillus casei Shirota probiotics for the treatment of traumatic ulcers in Wistar rats (Rattus norvegicus)

2021 ◽  
pp. 1279-1283
Author(s):  
Tuti Kusumaningsih ◽  
Anis Irmawati ◽  
Diah Savitri Ernawati ◽  
Chiquita Prahasanti ◽  
Mohammed Aljunaid ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Lactobacillus Casei ◽  
Wistar Rats ◽  
Systemic Administration ◽  
Control Group ◽  
Fibroblast Cells ◽  
Lactobacillus Casei Shirota ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
The Difference

Background and Aim: The use of drugs as a therapy for traumatic ulcers may lead to drug resistance and other side effects. Lactobacillus casei Shirota can affect the number of fibroblasts and blood vessels in wound healing. The aim of this study was to investigate the difference in the number of fibroblast cells and blood vessels after the topical and systemic administration of L. casei Shirota probiotics in Wistar rats with traumatic ulcer. Materials and Methods: Overall, 36 healthy male Wistar rats aged 2-3 months old and weighing 175-250 g in body weight were used as a sample. Traumatic ulcer was made on the labial fornix incisive inferior. The subject rats were divided into groups: (1) A control group over 3 days, (2) a group that used distilled water over 7 days, (3) a group that underwent topical treatment over 3 days, (4) a group that used probiotics administered topically over 7 days, (5) a group that underwent systemic treatment over 3 days, and (6) a group that took oral probiotics for the traumatic ulcers over 7 days. The number of fibroblasts and blood vessels was observed through a hematoxylin-eosin examination. Results: Based on the results of the study, a significant difference was observed in the number of fibroblasts (p=0.00) and blood vessels (p=0.018) in the 3-day topical group that underwent a 3-day systemic administration of probiotics compared with the number of fibroblast cells in the 7-day topical group and 7-day systemic group (p=0.00). Conclusion: Overall, significant differences were observed in the number of fibroblasts and blood vessels in Wistar rats with traumatic ulcer after undergoing the topical and systemic administration of L. casei Shirota probiotics.

Protective Effect of Boswellic Resin Against Memory Loss and Alzheimer’s Induced by Aluminum Tetrachloride and D-Galactose (Experimental study in Mice)

2020 ◽  
Author(s):  
K. Zerrouki ◽  
N. Djebli ◽  
L. Gadouche ◽  
I. Erdogan Orhan ◽  
F. SezerSenol Deniz ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Protective Effect ◽  
Cell Damage ◽  
Memory Loss ◽  
Control Group ◽  
Total Extract ◽  
Swiss Mice ◽  
Octyl Acetate

Nowadays, because of the industrialization, a lot of contaminant were available ; the consequences of this availability are apparition of diseases including neurodegeneration. Neurodegenerative diseases of the human brain comprise a variety of disorders that affect an increasing percentage of the population. This study is based on the effect of the Boswellic resin, which is from a medicinal plant and known for its antioxidant effects on nerve cell damage. The objective of this work was to evaluate the in vitro and in vivo effects of the Boswellic resin on anticholinesterase activity and Alzheimer’s disease (AD) induced by D-galactose and aluminum tetrachloride in Swiss mice. Chemical composition of the resin essential oil was identified by the CG-MS analysis. The antioxidant activity was also assessed by the DMPD and metal chelation methods. In order to understand the mechanism of memory improvement, the acetylcholinesterase, AChE, and butyrylcholinesterase, BChE, inhibitory assays were performed. In vivo part of the study was achieved on Swiss mice divided into four groups: control, AD model, treated AD, and treated control group. The identification of chemical composition by CG-MS reach the 89.67% of the total extract compounds presented some very important molecules (p-Cymene, n-Octyl acetate, α-Pinene…). The present study proves that Boswellic resin improves memory and learning in treated Alzheimer’s group, modulates the oxidative stress and be involved in the protective effect against amyloid deposition and neurodegeneration, and stimulates the immune system in mice’s brain.

PENGELUARAN KOLOSTRUM DENGAN PEMBERIAN PERAWATAN PAYUDARA DAN ENDORPHIN MASSAGE PADA IBU POST SEKSIO SESAREA

2019 ◽  
Vol 2 (1) ◽  
pp. 34-40
Author(s):  
Diah Evawanna Anuhgera ◽  
Eka Fitria Panjaitan ◽  
Desika Wali Pardede ◽  
Desika Wali Pardede ◽  
Nikmah Jalilah Ritonga ◽  
...  
Keyword(s):  
Experimental Study ◽  
Cesarean Section ◽  
Alternative Therapy ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
A Value ◽  
Quasi Experimental ◽  
Breast Care ◽  
June July

Spending colostrum in post-cesarean section mothers is difficult in the first week postpartum. Efforts to overcome colostrum disruption can use non-pharmacological methods, namely breast care and endorphin massage. This study aims to determine the expenditure of colostrum by giving breast care and endorphin massage to post-cesarean mothers. This research is a quasi-experimental study with a nonequivalent design posttest control group design. This research was carried out in the midwifery room of the second floor of Grand Medistra Lubuk Pakam Hospital during the June-July 2019 period. There were 48 participants selected using purposive sampling, with 18 assigned in breast care, endorphin massage and combination of breast care and endorphin massage. The time of spending colostrum expenditure is assessed every day while the amount of colostrum expenditure is assessed after intervening. Analysis of the data used is one-way Annova. The results showed that the combination of breast care and endorphin massage was the most effective action in the time of spending colostrum and the amount of colostrum with a value of p = 0,000. The combination of breast care and endorphin massage could be applied as an alternative therapy post-cesarean mothers    

scholarly journals Benefits of Dog-Assisted Therapy in Patients with Dementia Residing in Aged Care Centers in Spain

2021 ◽  
Vol 18 (4) ◽  
pp. 1471
Author(s):  
Eva Vegue Parra ◽  
Jose Manuel Hernández Garre ◽  
Paloma Echevarría Pérez
Keyword(s):  
Scientific Evidence ◽  
Alternative Therapy ◽  
Group Versus ◽  
Aged Care ◽  
Control Group ◽  
Controlled Clinical Trial ◽  
Neuropsychiatric Inventory ◽  
Randomized Controlled ◽  
Institutionalized Patients ◽  
Experimental Group

(1) Background: Currently, the scientific evidence on the benefits of assisted therapy with dogs in dementia is not clear. In this study, we want to evaluate such benefits through a randomized controlled clinical trial in multiple centers across the country. (2) Methods: The participants were people over 65 years old with dementia, residing in senior centers in Spain (n = 334). The experimental group underwent assisted therapy with dogs based on the Comprehensive Cognitive Activation Program in Dementia, for 8 months, with weekly sessions of 45 min. Data were collected at the commencement, middle, and end of the program, to evaluate the aspects using the Mini-Examination Cognitive, the modified Bartell Index, the Cornell Scale for Depression in Dementia and the Neuropsychiatric Inventory. (3) Results: The results show significant improvements in the experimental group versus the control group in the affective (T1 = p 0.000; T2 = p 0.000) and behavioral (T1 = p 0.005; T2 = p 0.000) aspects, with the affective aspect displaying greater progress in participants with additional depressive (p = 0.022) or anxiety (p = 0.000) disorders, shorter institutionalization periods (r = −0.222, p = 0.004), and those undergoing complementary psychotherapy (p = 0.033) or alternative therapy (p = 0.011). (4) Conclusions: Dog therapy is effective in improving the affective and behavioral aspects of institutionalized patients with dementia.

scholarly journals The Effectiveness of Probiotic Lactobacillus rhamnosus and Lactobacillus casei Strains in Children with Atopic Dermatitis and Cow’s Milk Protein Allergy: A Multicenter, Randomized, Double Blind, Placebo Controlled Study

Nutrients ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 1169
Author(s):  
Bożena Cukrowska ◽  
Aldona Ceregra ◽  
Elżbieta Maciorkowska ◽  
Barbara Surowska ◽  
Maria Agnieszka Zegadło-Mylik ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Lactobacillus Casei ◽  
Milk Protein ◽  
Lactobacillus Rhamnosus ◽  
Cow’S Milk ◽  
Controlled Study ◽  
Symptom Improvement ◽  
Cow's Milk ◽  
Probiotic Preparation ◽  
Cow’S Milk Protein

Probiotics seem to have promising effects in the prevention and treatment of allergic conditions including atopic dermatitis (AD) and food allergy. The purpose of this multicenter randomized placebo-controlled trial was to evaluate the effectiveness of a probiotic preparation comprising Lactobacillus rhamnosus ŁOCK 0900, Lactobacillus rhamnosus ŁOCK 0908, and Lactobacillus casei ŁOCK 0918 in children under 2 years of age with AD and a cow’s milk protein (CMP) allergy. The study enrolled 151 children, who—apart from being treated with a CMP elimination diet—were randomized to receive the probiotic preparation at a daily dose of 109 bacteria or a placebo for three months, with a subsequent nine-month follow-up. The primary outcomes included changes in AD symptom severity assessed with the scoring AD (SCORAD) index and in the proportion of children with symptom improvement (a SCORAD score decreased by at least 30% in comparison with that at baseline). After the three-month intervention, both the probiotic and placebo groups showed a significant (p < 0.0001) decrease in SCORAD scores, which was maintained nine months later. The percentage of children who showed improvement was significantly higher in the probiotic than in the placebo group (odds ratio (OR) 2.56; 95% confidence interval (CI) 1.13–5.8; p = 0.012) after three months. Probiotics induced SCORAD improvement mainly in allergen sensitized patients (OR 6.03; 95% CI 1.85–19.67, p = 0.001), but this positive effect was not observed after nine months. The results showed that the mixture of probiotic ŁOCK strains offers benefits for children with AD and CMP allergy. Further research is necessary to assess the effect of probiotic supplementation on the development of immune tolerance. (ClinicalTrials.gov NCT04738565)

scholarly journals PSVIII-8 Effect of Lactobacillus Casei Zhang on E. coli induced injury of blood and milk barrier in dairy cow

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 259-259
Author(s):  
Yuhui Zheng ◽  
Shengli Li
Keyword(s):  
Bacterial Infection ◽  
Lactobacillus Casei ◽  
Bacterial Resistance ◽  
Bovine Mastitis ◽  
Production Performance ◽  
Economic Losses ◽  
Control Group ◽  
Bovine Mammary Epithelial Cells ◽  
E Coli ◽  
Lactobacillus Casei Zhang

Abstract Bovine mastitis is one of the major diseases which directly affects the milk production performance and it causes huge economic losses in the dairy industry. Bacterial infection is the main risk factor of bovine mastitis and the antibiotic therapy is the primary choice to control the disease. However, persistence use of antibiotic increases the incidence of bacterial resistance and traces of antibiotic residues in animal products. Lactobacillus casei Zhang is one of the probiotics with multiple biological functions, which has certain bacteriostatic effect on pathogenic microorganism. The purpose of this study was to explore the effect of Lactobacillus casei Zhang (L. casei Zhang) on the prevention of E. coli-induced milk-blood barrier damage. Bovine mammary epithelial cells (BMECs) were used to establish a milk-blood model and Control group (PBS), E. coli group, and L. casei Zhang pretreatment plus E. coli group were set up respectively. The results showed that: L. casei Zhang could significantly reduce the increase of LDH release caused by E. coli treatment (P&lt; 0.05). And it can also significantly reduce the decrease of transmembrane resistance of monolayer cells caused by E. coli treatment (P&lt; 0.05). In addition, L. casei Zhang could significantly reduce the expression of tight junction proteins ZO-1, Claudin-1, Claudin-4 and Occludin (P &lt; 0.05). In conclusion, L. casei Zhang could effectively improve the damage of the blood-milk barrier caused by E. coli and could protect BMECs during bacterial infection.

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