scholarly journals Effect of various factors on anthocyanins extraction from butterfly pea flower (Clitoria ternatea L.)

2021 ◽  
Vol 20 (1) ◽  
pp. e817
Author(s):  
Dwi Aditiyarini ◽  
Ester Oktaviana Iswuryani
Keyword(s):  
Total Phenolic Content ◽  
Phenolic Content ◽  
Cell Damage ◽  
Extraction Time ◽  
Total Phenolic ◽  
Solvent Ratio ◽  
Water Solvent ◽  
Total Anthocyanins ◽  
Unstable Molecules ◽  
Butterfly Pea

An antioxidant compound is the main compound that is used to prevent cell damage from free radicals. These unstable molecules can be produced in the environmental condition such as pollution or lifestyle. One of the antioxidant molecules are anthocyanins, which can be found in the butterfly pea flower. This compound could be obtained from the extraction process. However, extraction conditions such as sample/solvent ratio, extraction time, and pH are the main factors in maximizing the yield. In this research, various factors on anthocyanins and phenolic content in butterfly pea extract were studied to get the optimum extraction condition. Extraction of the butterfly pea flower was done using the agitation method with heating and water solvent at 60 °C and various parameters. The sample was a dried butterfly pea flower. Various factors in extraction were: sample/solvent ratio, 1 : 20 and 1 : 50 (g.mL-1), extraction time of 90 and 150 min, and pH 1.0 and 7.0. Yield is calculated by comparing the extract weight before and after drying. Total anthocyanins content and total phenolic content are determined spectrophotometrically. Based on the results, the extraction of anthocyanins was affected by the stability of structures at different pH values. The highest total anthocyanins content was 1,206.77 mg.L-1 at sample/solvent ratio 1 : 20, 90 min and pH 1.0 conditions. Then, the maximum total phenolic content was 94.04 GAE mg.mg-1 sample at the sample/solvent ratio 1 : 50, 90 min and pH 7.0.

scholarly journals Effects of Extraction Methods and Conditions on Bioactive Compounds Extracted from Phaeodactylum tricornutum

2020 ◽  
Vol 67 (4) ◽  
pp. 1250-1261
Author(s):  
Saniye Akyıl ◽  
Işıl İlter ◽  
Mehmet Koç ◽  
Zeliha Demirel ◽  
Ayşegül Erdoğan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Extraction Method ◽  
Phaeodactylum Tricornutum ◽  
Phenolic Content ◽  
Extraction Methods ◽  
Extraction Time ◽  
Total Phenolic ◽  
Solvent Ratio ◽  
Microwave Extraction

The effect of homogenization, ultrasound and microwave extraction methods and conditions on fucoxanthin content, total phenolic content and antioxidant activity of extracts obtained from Phaeodactylum tricornutum were investigated in this study. The solvent/biomass ratio was the most effective parameter on fucoxanthin content, total phenolic content and antioxidant activity. The maximum fucoxanthin content (5.60 ± 0.06 mg/g) and antioxidant activity (763.00 ± 15.88 EC50 μg/mL extract) were obtained with the homogenization extraction method whose optimum conditions were 1.93% biomass/solvent ratio, ~5200 rpm homogenization rate and 14.2 min extraction time. Although the ultrasonic extraction method has reached the approximately same level of fucoxanthin content (5.24 ± 0.07 mg/g)), TPC (67.68 ± 1.58 mg gallic acid/L) and antioxidant activity (619.90 ± 17.16 EC50 μg/mL extract) at an amplitude of 55.72%, a higher biomass/solvent ratio (2.72%) and a longer extraction time (17.37 min) have been required. The lowest fucoxanthin content, total phenolic content and antioxidant activity were determined for the microwave extraction method.

Effects of Extraction Conditions on Total Phenolic Content and Total Flavonoid Content of Roselle (Hibiscus sabdariffa L.) Extracts

2019 ◽  
Vol 814 ◽  
pp. 469-474
Author(s):  
Nguyen Quoc Duy ◽  
Mai Le Thanh Binh ◽  
Ma Thuan ◽  
Ngo Thi Thanh Van ◽  
Tri Duc Lam ◽  
...  
Keyword(s):  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Flavonoid ◽  
Extraction Time ◽  
Total Phenolic ◽  
Solvent Ratio ◽  
Extraction Temperature ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Time Duration

The objective of this study was to determine the optimal value of factors affecting the extraction of compounds such as, polyphenols, flavonoids of Roselle. The investigated factors included extraction temperatures (30, 40, 50, 60, 70 and 80°C), extraction time (30, 60, 90, 120 and 150 minutes) and solid to solvent ratio (1:5, 1:10, 1:20, 1:40 and 1:50 g/mL). The efficiency of extraction was evaluated based on total phenolic content (TPC) and total flavonoid content (TFC). The results showed that temperature range of 60–80°C was the appropriate temperature for TFC while extraction temperature over 60°C was suitable for achieving highest TPC. Considering the effect of extraction time, duration of 90–150 minutes facilitated the extraction of both phenolics and flavonoids. Moreover, the solid to solvent 1:20 was chosen for extraction process based on the balance of antioxidant contents and their yield.

scholarly journals Optimisation of Organic Solvent Mediated Solubilisation of Apple Pomace Polyphenolic Compounds Using Response Surface Methodologies

2019 ◽  
Vol 11 (2) ◽  
pp. 1
Author(s):  
Salis Ibrahim ◽  
Regina Santos ◽  
Steve Bowra
Keyword(s):  
Total Phenolic Content ◽  
Phenolic Content ◽  
Polyphenolic Compounds ◽  
Apple Pomace ◽  
Extraction Time ◽  
Total Phenolic ◽  
Solvent Ratio ◽  
Acetone Concentration ◽  
Solvent Type ◽  
Time Optimal

Polyphenolic compounds extraction from industrial apple pomace was optimised by applying design of experiments (DoE) and surface response methodology using the Central Composite Rotatable Design (CCRD). The degree solubilisation and the yield of total phenolic content from the apple pomace using organic solvents was shown to be influenced by process parameters including solvent type, solvent concentration, temperature, apple pomace to solvent ratio, and extraction time (residency time). Optimal conditions of extracting phenolic compounds were as follows: acetone concentration, 65 % (v/v); solid to solvent ratio 1 %; extraction time 30 minutes and temperature 60oC. Optimum condition for solubilisation was as follows: acetone concentration 78 % (v/v); solid to solvent ratio 4.7 %; extraction time 54 minutes and temperature 21oC. Under these conditions, the total phenolic content and solubilisation were 21.70 ± 0.2 mg GAE/g dw and 19.20 ± 0.1g/100g of the dried apple pomace respectively and largely agreed with those predicted by the Stat-Ease software. Independent variables for optimisation of total phenolic content and solubilisation were completely different. The reverse phase HPLC analysis of the extract revealed the major polyphenolic compounds were chlorogenic acid, procyanidin B2, caffeic acid, epicatechin, ferulic acid, quercetin-3-galactoside, quercetin-3-glucoside and phloridzin.

Ultrasound-Assisted Extraction of Polyphenols from Pomelo (Citrus Grandis Limonia Osbeck L.) Peel

2021 ◽  
Vol 31 (4) ◽  
pp. 32-37
Keyword(s):  
Antioxidant Capacity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Solvent Ratio ◽  
Sonication Time ◽  
Ultrasound Assisted Extraction ◽  
Total Polyphenol ◽  
Pomelo Peel ◽  
Assisted Extraction

The pomelo peel occupies 50% of the fruit mass in pomelo juice processing. It contains large amounts of phenolic compounds, which may provide benefits to human health. These components should be isolated. In this study, the effects of ethanol concentrations, material-to-solvent ratios (g/mL), temperatures and sonication time on total phenolic content (TPC), naringin content and antioxidant capacity (using DPPH assay) of extract solution was evaluated. The results showed that all experimental factors significantly influenced the extraction of total polyphenol content, naringin content, and antioxidant capacity of the extract. The extraction condition was ethanol 80%, material-to-solvent ratio of 1:25 (w/v) at 60oC, and sonication time of 7.5 min, gave the extract had total phenolic content of 9.05 ± 0.08 mg GAE/g DM, naringin content of 4.65 ± 0.08 mg NE/ g DM, and antioxidant capacity of 4.76 ± 0.03 mg AAE/g DM. The ultrasound treatment was a useful method for improving the extraction of phenolic acid compounds from pomelo peel.

scholarly journals Ultrasonic-Assisted Extraction (UAE) for Enhanced Recovery of Bioactive Phenolic Compounds From Cosmos Caudatus Leaves

2021 ◽  
Author(s):  
NORLIZA LATIFF ◽  
PEI YING ONG ◽  
LUQMAN CHUAH ABDULLAH ◽  
SITI NOR AZLINA ABD RASHID ◽  
NOOR AKHMAZILLAH MOHD FAUZI ◽  
...  
Keyword(s):  
Particle Size ◽  
Bioactive Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Extraction Time ◽  
Total Phenolic ◽  
Cosmos Caudatus ◽  
Assisted Extraction ◽  
Soxhlet Method ◽  
Microbial Agents

Abstract Cosmos caudatus (C. caudatus) is one of the common medicinal plants and among the valuable plants that are high in bioactive compounds such as phenolics. In this study, an ultrasound-assisted extraction (UAE) method was used to optimise the extraction of bioactive compounds from C. caudatus leaves using response surface methodology (RSM) and employing a Box-Behnken experimental design (BBD). The extraction efficiency of UAE under the optimal extraction conditions was compared with the Soxhlet method. Also, an anti-microbial analysis against two human pathogenic bacteria; Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) was also evaluated. The effect of the extraction conditions was studied and optimised such as the solid-liquid ratio (10 to 30 g/ml), particle size (180 to 850 µm) and extraction time (20 to 30 min). Quercitrin and total phenolic content (TPC) were the selected response variables in this study. Based on the ANOVA analysis, the response surface model to predict the optimum yield of quercitrin and TPC was adequate with a high R-square value corresponding to 0.9930 and 0.9962. The optimal UAE conditions were 1:28 (g/mL), by using a particle size of 485 µm and an extraction time of 30 min, respectively. Remarkably, UAE reduced the extraction time and solvent volume, with the maximal recovery of bioactive compounds at a high antioxidant activity as compared with that of the Soxhlet method. The plant extract also exhibited potential microbial agents. Due to the above findings, UAE can be used to enrich quercitrin and total phenolic content from C. caudatus leaves. It also opens the possibility of plant extract to be used as an affordable component in many applications such as food formulations and anti-microbial agents.

scholarly journals Anthocyanin Pigment and Total Phenolic Content of Three Vaccinium Species Native to the Pacific Northwest of North America

HortScience ◽  
2004 ◽  
Vol 39 (5) ◽  
pp. 959-964 ◽  
Author(s):  
Jungmin Lee ◽  
Chad E. Finn ◽  
Ronald E. Wrolstad
Keyword(s):  
Pacific Northwest ◽  
Total Phenolic Content ◽  
High Performance ◽  
Phenolic Content ◽  
Total Phenolics ◽  
Total Phenolic ◽  
Anthocyanin Content ◽  
Anthocyanin Pigment ◽  
Total Anthocyanin ◽  
Total Anthocyanins

The total anthocyanin and total phenolic content of wild (samples from 4 populations) and cultivated (samples from 32 populations) Pacific Northwestern American Vaccinium species (V. membranaceum, V. ovalifolium, and V. deliciosum) were evaluated. The total monomeric anthocyanin content of all huckleberry samples analyzed ranged from 101 to 400 mg/100 g (expressed as cyanidin-3-glucoside), and the total phenolics varied from 367 to 1286 mg/100 g (expressed as gallic acid). Cluster analysis separated the samples into four different groups based on their anthocyanin and total phenolic content. Two groups had greater anthocyanin pigment and total phenolics; one consisted entirely of cultivated V. ovalifolium (LIG10, VAC485, VAC487, LIG33, LIG9, LIG2, and VAC349) and the other consisted of just cultivated V. membranaceum (LIG25). Significant variations in total anthocyanins, total phenolics, and the ratio of the total anthocyanins and total phenolics were observed among the different V. membranaceum, V. ovalifolium, and V. deliciosum populations cultivated in the Willamette Valley, Ore. The profile of the individual anthocyanins of the wild V. membranaceum, wild V. ovalifolium, and V. corymbosum `Rubel' were conducted by high-performance liquid chromatography. The chromatograms of V. membranaceum, V. ovalifolium, and `Rubel' were distinctly different in the amounts of delphinidin, cyanidin, and malvidin glycosides.

scholarly journals Optimization of Polyphenol Extraction from Allium ampeloprasum var. porrum through Response Surface Methodology

Foods ◽  
2018 ◽  
Vol 7 (10) ◽  
pp. 162 ◽  
Author(s):  
Irini Strati ◽  
George Kostomitsopoulos ◽  
Fotios Lytras ◽  
Panagiotis Zoumpoulakis ◽  
Charalampos Proestos ◽  
...  
Keyword(s):  
Total Phenolic Content ◽  
Plant Material ◽  
Phenolic Content ◽  
Solvent Mixture ◽  
Extraction Time ◽  
Total Phenolic ◽  
Leaf Extracts ◽  
Factors Affecting ◽  
Allium Ampeloprasum ◽  
Mediterranean Countries

Allium ampeloprasum var. porrum has been recognized as a rich source of secondary metabolites, including phenolic acids, flavonoids and flavonoid polymers (proanthocyanidins or condensed tannins), with related health benefits. Both parts of Allium ampeloprasum var. porrum (white bulb and pseudostem) are traditionally consumed either as a vegetable or as a condiment in many Mediterranean countries. The aim of the present study was to optimize the extraction conditions of polyphenols from white leek stem and green leek leaf by implementing a Box-Behnken design (BBD). The optimization considered basic factors affecting extraction efficiency, including extraction time, solvent to plant material ratio and solvent mixture composition. Maximum polyphenol yield was achieved at an extraction time of 80 and 100 min for white leek stem and green leek leaf extracts respectively, solvent to plant material ratio of 5:1 (v/w) and methanol to water ratio of 40:60 (v/v), for both leek extracts. Interestingly, higher total phenolic content was found in green leek leaf extracts compared to white leek stem extracts, due to a possible relationship between polyphenol production and sunlight radiation. High correlation values were also observed between total phenolic content and antioxidant-antiradical activity of optimized leek extracts.

scholarly journals Microwave-assisted extraction of phenolic compounds from Moringa oleifera seed as anti-biofouling agents in membrane processes

2018 ◽  
Vol 204 ◽  
pp. 03003 ◽  
Author(s):  
Ni'matul Izza ◽  
Shinta Rosalia Dewi ◽  
Ashried Setyanda ◽  
Agung Sukoyo ◽  
Panggulu Utoro ◽  
...  
Keyword(s):  
Moringa Oleifera ◽  
Phenolic Content ◽  
Extraction Time ◽  
Total Phenolic ◽  
Solvent Ratio ◽  
Microwave Assisted Extraction ◽  
Purification Process ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Moringa Oleifera Seed

Moringa seed has known as a coagulant in the water purification process. It is because of the large amount of anti-microbial compounds contained in it. Phenol is one of the most common anti-microbial compounds found in natural materials. The aim of this study was to determine the total phenolic content (TPC) of Moringa seed which was extracted by Microwave-Assisted Extraction (MAE). Moringa seeds were characterized by FTIR and showed that it contained phenol compounds confirmed by specific peak in some areas. Moringa seeds were characterized by FTIR before extracted. Therefore, MAE was performed by variation of solvent ratios (1: 4, 1: 6, 1: 8) and extraction time (2, 3, 4 min). The highest TPC of 41.78 mg GAE / g dw was reached at 1: 8 solvent ratio and 3-min extraction time.

Chemical characterization of ethanolic extract of Butterfly pea flower (Clitoria ternatea)

Food Research ◽  
2021 ◽  
Vol 5 (4) ◽  
pp. 127-134
Author(s):  
T.N.M. Tuan Putra ◽  
M.K. Zainol ◽  
N.S. MohdIsa ◽  
N. MohdMaidin
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Light Exposure ◽  
Ethanolic Extract ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Clitoria Ternatea ◽  
Dpph Method ◽  
Butterfly Pea

Clitoria ternatea, commonly known as Butterfly pea flower or Bunga telang, is a tropical flower and notable as one of the most vital sources of polyphenols with high antioxidant capacity. C. ternatea abundantly produces anthocyanin compounds benefiting natural blue colourants. To date, a blue colourant is the most difficult to obtain since the anthocyanins responsible for this colour is the least stable and easily degraded after extraction. Their stability commonly relies on several operational parameters, such as shifted pH value, high temperature, and light exposure. Apart from colour attributes, anthocyanins have also been identified as a source of functional molecules due to antioxidant activity and beneficial health effects such as anti-cancer, anti-obesity, antimicrobial and more. This study aimed to characterize the chemical constituents of the extractable polyphenols from ethanolic extract of C. ternatea quantitatively. The numerical data were evaluated using the Minitab version 18 statistical method. The chemical profiling, including total phenolic content (TPC), total flavonoid content (TFC), total monomeric anthocyanins (TMA) were determined using standard methods. The antioxidant activity was evaluated using DPPH and ABTS methods. The results showed that the ethanolic extract of C. ternatea had 35.7 mg QE/g of TFC, 102.4 mg GAE/g280nm and 28.8 mg GAE/g750nm of TPC and 2.7 CE/g and 2.9 ME/g of TMA. The total phenolic content of C. ternatea showed a good correlation with the antioxidant activity by the DPPH method when analysed using Pearson correlation and showed an increasing trend of antioxidant power with regards to TPC in both assays. In a nutshell, this study contributes to the knowledge of ethanolic extract of C. ternatea. Future research could consider exploring other extraction methods that could enhance the stability of polyphenols particularly the anthocyanins from degradation such as using surfactants.

Natural Deep Eutectic Solvents (NaDESs) as Alternative Green Extraction Media for Ginger (Zingiber officinale Roscoe)

2021 ◽  
Vol 2 (4) ◽  
pp. 576-599
Author(s):  
Andromachi Tzani ◽  
Styliani Kalafateli ◽  
Grigorios Tatsis ◽  
Maria Bairaktari ◽  
Ioanna Kostopoulou ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Zingiber Officinale ◽  
Deep Eutectic Solvents ◽  
Extraction Time ◽  
Total Phenolic ◽  
Optimum Conditions ◽  
Natural Deep Eutectic Solvents ◽  
Zingiber Officinale Roscoe

The extraction of valuable phytochemicals from natural sources is an important and constantly evolving research area. Zingiber officinale Roscoe (ginger) contains high amounts of bioactive phytochemicals, which are desirable due to their significant properties. In this work, the ability of different natural deep eutectic solvents (NaDESs) to serve as green solvents for the preparation of high added value extracts from ginger is explored, in combination with ultrasound assisted extraction. The method was optimized by applying a response surface methodology using the NaDES Bet/La/W (1:2:2.5). Three independent variables, namely the extraction time, ultrasound power and NaDES-to-dry-ginger ratio, were investigated by employing a 17-run three-level Box–Behnken Design (BBD) in order to study the correlation between the extraction conditions and the quality of the obtained extracts. The optimum conditions (in order to achieve simultaneously maximum total phenolic content and antioxidant activity), were found to be 23.8 min extraction time, 60 Watt and NaDES/ginger 25:1 w/w. In the optimum conditions the DPPH radical scavenging ability of the extracts was found to reach IC50 = 18.16 mg/mL after 120 min, whereas the TPC was 20.10 ± 0.26 mg GAE/g of dry ginger. The green methodology was also compared with the extraction using conventional solvents. All the obtained extracts were evaluated for their antioxidant activity and their total phenolic content, while the extract derived by the optimum extraction conditions was further investigated for its ability to bind to calf thymus DNA (ctDNA).

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