scholarly journals Quantification of anti-quorum sensing and antibiofilm activity of Phyllosphere bacteria against food spoilage bacteria

2021 ◽  
pp. e919
Author(s):  
Valencia Vanessa ◽  
Diana Elizabeth Waturangi
Keyword(s):  
Quorum Sensing ◽  
Bioactive Compounds ◽  
Large Population ◽  
Cell Communication ◽  
Antibiofilm Activity ◽  
Food Spoilage ◽  
Biofilm Inhibition ◽  
Sensing Mechanism ◽  
Spoilage Bacteria ◽  
Phyllosphere Bacteria

Food spoilage and microbial contamination require  attention during the food production process since the presence of these bacteria can create problems including the formation of biofilms produced by these  bacteria. Biofilm formations are initiated through cell-to-cell communication which is called quorum sensing mechanism. Hence, inhibition of this communication  mechanism could be one of the solutions to inhibit  biofilm formation. Therefore, exploration of bioactive compounds from various sources including  hyllosphere bacteria with anti-quorum sensing inhibition activities is important. Phyllosphere bacteria are a community of bacteria found on the surface of plant leaves at a very  large population. These bacteria can produce bioactive compounds that can inhibit quorum sensing mechanism. In this study, 54 phyllosphere bacteria  isolates were tested, 8 bacterial isolates had potential effect to inhibit quorum sensing. From biofilm inhibition assay, the highest percentages were showed by  ifferent phyllosphere isolates against each pathogen. Whereas, for biofilm destruction assay, JB 8F isolate had the highest percentage of destruction biofilm activity  against biofilm formed by Bacillus cereus and  Shewanella putrefaciens. Eight isolates of phyllosphere  bacteria had the potential as quorum quencher and  anti-biofilm agents, both for inhibition and destruction of biofilm.  

scholarly journals Peptide Mix from Olivancillaria hiatula Interferes with Cell-to-Cell Communication in Pseudomonas aeruginosa

2019 ◽  
Vol 2019 ◽  
pp. 1-12
Author(s):  
Edward Ntim Gasu ◽  
Hubert Senanu Ahor ◽  
Lawrence Sheringham Borquaye
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Quorum Sensing ◽  
Biofilm Formation ◽  
Antimicrobial Agents ◽  
Cell Communication ◽  
Microtiter Plate ◽  
Antibiofilm Activity ◽  
Dependent Manner ◽  
Biofilm Inhibition ◽  
Swarming Motility

Bacteria in biofilms are encased in an extracellular polymeric matrix that limits exposure of microbial cells to lethal doses of antimicrobial agents, leading to resistance. In Pseudomonas aeruginosa, biofilm formation is regulated by cell-to-cell communication, called quorum sensing. Quorum sensing facilitates a variety of bacterial physiological functions such as swarming motility and protease, pyoverdine, and pyocyanin productions. Peptide mix from the marine mollusc, Olivancillaria hiatula, has been studied for its antibiofilm activity against Pseudomonas aeruginosa. Microscopy and microtiter plate-based assays were used to evaluate biofilm inhibitory activities. Effect of the peptide mix on quorum sensing-mediated processes was also evaluated. Peptide mix proved to be a good antibiofilm agent, requiring less than 39 μg/mL to inhibit 50% biofilm formation. Micrographs obtained confirmed biofilm inhibition at 1/2 MIC whereas 2.5 mg/mL was required to degrade preformed biofilm. There was a marked attenuation in quorum sensing-mediated phenotypes as well. At 1/2 MIC of peptide, the expression of pyocyanin, pyoverdine, and protease was inhibited by 60%, 72%, and 54%, respectively. Additionally, swarming motility was repressed by peptide in a dose-dependent manner. These results suggest that the peptide mix from Olivancillaria hiatula probably inhibits biofilm formation by interfering with cell-to-cell communication in Pseudomonas aeruginosa.

scholarly journals Screening and quantification of anti-quorum sensing and antibiofilm activity of Actinomycetes isolates against food spoilage biofilm-forming bacteria

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Erika Mulya ◽  
Diana Elizabeth Waturangi
Keyword(s):  
Public Health ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Quorum Sensing ◽  
Food Industry ◽  
Economic Losses ◽  
Antibiofilm Activity ◽  
Food Spoilage ◽  
Crude Extracts ◽  
Spoilage Bacteria

Abstract Background Biofilms can form in many industries, one of them is the food industry. The formation of biofilms in this industry could cause immense economic losses and endanger public health. Biofilms formation is mainly triggered by quorum sensing. Therefore, inhibition of quorum sensing could be an innovative approach to inhibit the formation of biofilms. One way to inhibit quorum sensing is by using anti-quorum sensing compounds. Actinomycetes are a group of bacteria that is acknowledged to produce these compounds. Results There were eight crude extracts of Actinomycetes isolates that showed promising anti-quorum sensing activity against Chromobacterium violaceum. The concentration of the crude extracts was 20 mg/mL. All the crude extracts showed no antibacterial activity against food spoilage bacteria, except for crude extracts of isolate 18 PM that showed antibacterial activity against Bacillus subtilis. They also showed various antibiofilm activity, both inhibition and destruction. The highest inhibition and destruction activity sequentially was done by crude extracts of isolate 12 AC with 89.60% against Bacillus cereus and crude extracts of isolate SW03 with 93.06% against Shewanella putrefaciens. Conclusions Actinomycetes isolates that isolated from different regions in Indonesia can be used as potential candidates to overcome biofilms formed by food spoilage bacteria using their ability to produce anti-quorum sensing compounds.

scholarly journals Mechanisms and Impact of Biofilms and Targeting of Biofilms Using Bioactive Compounds—A Review

Medicina ◽  
2021 ◽  
Vol 57 (8) ◽  
pp. 839
Author(s):  
Antony V Samrot ◽  
Amira Abubakar Mohamed ◽  
Etel Faradjeva ◽  
Lee Si Jie ◽  
Chin Hooi Sze ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Bioactive Compounds ◽  
Biofilm Formation ◽  
Antimicrobial Agents ◽  
Cell Communication ◽  
Vital Role ◽  
Control Measures ◽  
Biofilm Inhibition ◽  
Antibiofilm Agents ◽  
And Control

Biofilms comprising aggregates of microorganisms or multicellular communities have been a major issue as they cause resistance against antimicrobial agents and biofouling. To date, numerous biofilm-forming microorganisms have been identified, which have been shown to result in major effects including biofouling and biofilm-related infections. Quorum sensing (which describes the cell communication within biofilms) plays a vital role in the regulation of biofilm formation and its virulence. As such, elucidating the various mechanisms responsible for biofilm resistance (including quorum sensing) will assist in developing strategies to inhibit and control the formation of biofilms in nature. Employing biological control measures (such as the use of bioactive compounds) in targeting biofilms is of great interest since they naturally possess antimicrobial activity among other favorable attributes and can also possibly act as potent antibiofilm agents. As an effort to re-establish the current notion and understanding of biofilms, the present review discuss the stages involved in biofilm formation, the factors contributing to its development, the effects of biofilms in various industries, and the use of various bioactive compounds and their strategies in biofilm inhibition.

scholarly journals Screening and quantification of anti-quorum sensing and antibiofilm activities of phyllosphere bacteria against biofilm forming bacteria

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Nadine Amabel Theodora ◽  
Vania Dominika ◽  
Diana Elizabeth Waturangi
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Quorum Sensing ◽  
Crude Extract ◽  
Pathogenic Bacteria ◽  
Antibiofilm Activity ◽  
Biofilm Inhibition ◽  
Crude Extracts ◽  
Turbid Zone ◽  
Phyllosphere Bacteria

Abstract Objective The objectives of this research were to screen anti-quorum sensing activity of phyllosphere bacteria and quantify their antibiofilm activity against biofilm forming bacteria (Bacillus cereus, Staphylococcus aureus, Enterococcus faecalis, Salmonella typhimurium, Vibrio cholerae, Pseudomonas aeruginosa). Results We found 11 phyllosphere bacteria isolates with potential anti-quorum sensing activity. Most of the crude extracts from phyllosphere bacteria isolates had anti-quorum sensing activity against Chromobacterium violaceum at certain concentration (20 and 10 mg/mL), but not crude extract from isolate JB 7F. Crude extract showed the largest turbid zone (1,27 cm) using isolate JB 14B with concentration of 10 mg/mL and the narrowest turbid zone isolate (1 cm) using JB 18B with concentration of 10 mg/mL. Crude extracts showed various antibiofilm activities against all tested pathogenic bacteria, it showed the highest biofilm inhibition (90%) and destruction activities (76%) against S. aureus.

Bacterial Foraging Optimization Algorithm with Quorum Sensing Mechanism

2014 ◽  
Vol 556-562 ◽  
pp. 3844-3848
Author(s):  
Hai Shen ◽  
Mo Zhang
Keyword(s):  
Quorum Sensing ◽  
Optimization Algorithm ◽  
Complex Adaptive Systems ◽  
Adaptive Systems ◽  
Cell Communication ◽  
Bacterial Foraging Optimization ◽  
Sensing Mechanism ◽  
Bacterial Foraging ◽  
Bacterial Quorum ◽  
Bacterial Foraging Optimization Algorithm

Quorum sensing is widely distributed in bacteria and make bacteria are similar to complex adaptive systems, with intelligent features such as emerging and non-linear, the ultimate expression of the adaptive to changes in the environment. Based on the phenomenon of bacterial quorum sensing and Bacterial Foraging Optimization Algorithm, some new optimization algorithms have been proposed. In this paper, it presents research situations, such as environment-dependent quorum sensing mechanism, quorum sensing mechanism with quantum behavior, cell-to-cell communication, multi-colony communication, density perception mechanism. Areas of future emphasis and direction in development were also pointed out.

scholarly journals The in Vitro Antibiofilm Activity of Waterfall and Marine Bacteria Against Human Bacterial Pathogens

2020 ◽  
Vol 13 (2) ◽  
pp. 250-262
Author(s):  
Stella Magdalena ◽  
Natassa Rustandi ◽  
Yogiara Yogiara
Keyword(s):  
Quorum Sensing ◽  
16S Rrna ◽  
Bioactive Compounds ◽  
Marine Bacteria ◽  
Bacterial Pathogens ◽  
Quorum Quenching ◽  
Health Concern ◽  
Effective Control ◽  
Antibiofilm Activity ◽  
Antibiofilm Agents

AbstrakKeterlibatan biofilm pada infeksi kronis dan pada permukaan peralatan medis selalu menjadi wacana penting bagi kesehatan umum di dunia. Biofilm bakteri berkaitan dengan tingkat resistensi terhadap antibiotik yang menjadikan infeksi sulit untuk diobati. Untuk mengatasi masalah ini, pengendalian yang efektif perlu diimplementasikan, seperti penerapan senyawa antibiofilm. Beberapa tahun terakhir, lingkungan akuatik menjadi salah satu sumber potensi penghasil senyawa bioaktif, termasuk senyawa antibiofilm. Tujuan dari penelitian ini yaitu menapis dan mengkarakterisasi bakteri asal air terjun dan laut yang diperoleh dari beberapa lokasi di Indonesia, sebagai penghasil aktivitas antibiofilm. Isolat dievaluasi berdasarkan kemampuan aktivitas antimikroba terhadap enam bakteri patogen dan diikuti dengan penapisan senyawa antibiofilm. Sebanyak 11 dari 65 isolat menunjukkan aktivitas quorum sensing atau quorum quenching, dan hanya terdapat satu isolat yang memiliki aktivitas keduanya. Supernatan kesebelas isolat menunjukkan penghambatan pembentukan biofilm setidaknya terhadap satu patogen dengan metode uji biofilm statis. Karakterisasi senyawa bioaktif dari lima isolat yang terpilih menunjukkan aktivitas senyawa yang berbeda, seperti karbohidrat, protein, dan asam nukleat. Sekuensing gen penyandi 16S rRNA menetapkan kelima isolat tersebut berada dalam dua genus yang berbeda, Vibrio (WK2.4, WK2.6, and WK2.3) dan Pseudomonas (S1.2 dan S1.3). Penelitian ini memberikan wawasan baru terhadap pencarian kandidat bakteri akuatik sebagai agen antibiofilm yang potensial. Abstract Biofilm involvement in chronic infections and on the surface of medical equipments have been considered as public health concern worldwide. Bacterial biofilm is related to antibiotic resistance that made the diseases difficult to treat. An effective control strategy should be implemented, for example, by applying antibiofilm agents. Recently, concerns has been given to aquatic environment as potential sources of bioactive compounds, including the antibiofilm compounds. This study aimed to screen and characterize waterfall and marine bacteria obtained from several locations in Indonesia which have antibiofilm activity. The isolates were first evaluated for their antimicrobial activity against six bacterial pathogens and followed by antibiofilm screening. Eleven out of 65 isolates showed quorum sensing or quorum quenching activity, and one of them showed both activities. Supernatants of 11 isolates inhibited biofilm formation of at least one pathogen by using static biofilm assay. Bioactive compounds characterization of the selected five isolates revealed the presence of different compounds, such as carbohydrates, proteins, and nucleic acids. The 16S rRNA gene sequencing analysis classified five isolates into two different genera, Vibrio (WK2.4, WK2.6, and WK2.3) and Pseudomonas (S1.2 and S1.3). The present study provides insights into the discovery of aquatic bacteria candidates as antibiofilm agents.

Antibacterial and antibiofilm activity of the bacteriocin-producing strain Leuconostoc mesenteroides CHBY46 isolated from Algerian dromedary milk against Pseudomonas aeruginosa and Staphylococcus aureus biofilms

2019 ◽  
Vol 8 (4) ◽  
pp. 120-131
Author(s):  
Yahia Bellil ◽  
Zineb Benmechernene ◽  
Wassila Chahrour Bellil ◽  
Mebrouk Kihal
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
16S Rrna Gene Sequencing ◽  
Antibiofilm Activity ◽  
Rrna Gene ◽  
Biofilm Inhibition ◽  
Staph Aureus ◽  
Spoilage Bacteria ◽  
Dromedary Milk ◽  
And Staphylococcus Aureus

In order to control biofilm formation of pathogenic and spoilage bacteria in foods, some species of Leuconostoc are very important in food industries, as they increase the shelf life of foods during preservation. In this study the strain CHBY46 a bacteriocin-producing strain belonging to Leuconostoc ge-nus isolated from dromedary milk in the south of Algeria was characterized by 16S rRNA gene sequencing and MALDITOF MS mass spectrometry, tested for its antibacterial and antibiofilm activities against Pseudomonas aeruginosa and Staphylococcus aureus. The produced bacteriocin was partially puri-fied with sulfate ammonium precipitation and RP-HPLC. The strain CHBY46 was classified as Leuc. mesenteroides after molecular identification. Among the bacteria tested the pathogens Staph. aureus ATCC 29213 and Ps. aeru-ginosa ATCC 27653 were sensitive to this bacteriocin with 480 AU/ml. Antibi-ofilm activity was investigated by crystal violet assay. The bacteriocin of Leuc. mesenteroides CHBY 46 exhibited significant biofilm inhibition ; 35.58% with Ps. aeruginosa, and 42.11% with Staph. aureus. Tricine-SDS-PAGE analysis of the partially purified bacteriocin indicated a low molecular weight of approximately 3.5 kDa. Therfore, we conclude that bacteriocins from Leuco-nostoc have the potential as a therapeutic strategy against pathogen’s bio-films, which contribute, to bacterial pathogenicity and resistance toward antibiotics or being used in foods as adjunsts to contribute food safety.

Mining marine metagenomes revealed a quorum-quenching lactonase with improved biochemical properties that inhibits the food spoilage bacteria Pseudomonas fluorescens

2021 ◽  
Author(s):  
Rami Haramati ◽  
Shlomit Dor ◽  
David Gurevich ◽  
Doron Levy ◽  
Dekel Freund ◽  
...  
Keyword(s):  
Pseudomonas Fluorescens ◽  
Marine Environment ◽  
Recombinant Expression ◽  
Cell Communication ◽  
Bacterial Pathogen ◽  
Quorum Quenching ◽  
Amino Acid Identity ◽  
Biochemical Properties ◽  
Food Spoilage ◽  
Spoilage Bacteria

The marine environment presents great potential as a source of microorganisms that possess novel enzymes with unique activities and biochemical properties. Examples of such are the quorum-quenching (QQ) enzymes that hydrolyze bacterial quorum-sensing (QS) signaling molecules, such as N-acyl-homoserine lactones (AHLs). QS is a form of cell-to-cell communication that enables bacteria to synchronize gene expression in correlation with population density. Searching marine metagenomes for sequences homologous to an AHL lactonase from the phosphotriesterase-like lactonase (PLL) family, we identified new putative AHL lactonases (sharing 30-40% amino acid identity to a thermostable PLL member). Phylogenetic analysis indicated that these putative AHL lactonases comprise a new clade of marine enzymes in the PLL family. Following recombinant expression and purification, we verified the AHL lactonase activity for one of these proteins, named marine originated Lactonase Related Protein (moLRP). This enzyme presented greater activity and stability at a broad range of temperatures and pH, and tolerance to high salinity levels (up to 5M NaCl), as well as higher durability in bacterial culture, compared to another PLL member. The addition of purified moLRP to cultures of Pseudomonas fluorescens inhibited its extracellular protease activity, expression of the protease encoding gene, biofilm formation, and the sedimentation process in milk-based medium. These findings suggest that moLRP is adapted to the marine environment, and can potentially serve as an effective QQ enzyme, inhibiting the QS process in gram-negative bacteria involved in food spoilage. Importance Our results emphasize the potential of sequence and structure-based identification of new quorum-quenching (QQ) enzymes from environmental metagenomes, such as from the ocean, with improved stability or activity. The findings also suggest that purified QQ enzymes can present new strategies against food spoilage, in addition to their recognized involvement in inhibiting bacterial pathogen virulence factors. Future studies on the delivery and safety of enzymatic QQ strategy against bacterial food spoilage should be performed.

scholarly journals Quorum Sensing in the Context of Food Microbiology

2012 ◽  
Vol 78 (16) ◽  
pp. 5473-5482 ◽  
Author(s):  
Panagiotis N. Skandamis ◽  
George-John E. Nychas
Keyword(s):  
Food Safety ◽  
Quorum Sensing ◽  
Communication Systems ◽  
Cell Communication ◽  
Food Preservation ◽  
Food Microbiology ◽  
Limited Information ◽  
Food Spoilage ◽  
The Many

ABSTRACTFood spoilage may be defined as a process that renders a product undesirable or unacceptable for consumption and is the outcome of the biochemical activity of a microbial community that eventually dominates according to the prevailing ecological determinants. Although limited information are reported, this activity has been attributed to quorum sensing (QS). Consequently, the potential role of cell-to-cell communication in food spoilage and food safety should be more extensively elucidated. Such information would be helpful in designing approaches for manipulating these communication systems, thereby reducing or preventing, for instance, spoilage reactions or even controlling the expression of virulence factors. Due to the many reports in the literature on the fundamental features of QS, e.g., chemistry and definitions of QS compounds, in this minireview, we only allude to the types and chemistry of QS signaling moleculesper seand to the (bioassay-based) methods of their detection and quantification, avoiding extensive documentation. Conversely, we attempt to provide insights into (i) the role of QS in food spoilage, (ii) the factors that may quench the activity of QS in foods and review the potential QS inhibitors that might “mislead” the bacterial coordination of spoilage activities and thus may be used as biopreservatives, and (iii) the future experimental approaches that need to be undertaken in order to explore the “gray” or “black” areas of QS, increase our understanding of how QS affects microbial behavior in foods, and assist in finding answers as to how we can exploit QS for the benefit of food preservation and food safety.

scholarly journals Biofilm-Associated Agr and Sar Quorum Sensing Systems of Methicillin-Resistant Staphylococcus Aureus are Inhibited by Fruit Extracts of Illicium Verum

2019 ◽  
Author(s):  
Pitchaipillai Sankar Ganesh ◽  
Veena Krishnamurthy ◽  
Koneti ISwamy ◽  
Suvaiyarasan Suvaithenamudhan ◽  
Murugesan Amuthan ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Molecular Docking ◽  
Quorum Sensing ◽  
Light Microscopy ◽  
Major Constituent ◽  
Antibiofilm Activity ◽  
Systems Methodology ◽  
Biofilm Inhibition ◽  
Swarming Motility

Background: Staphylococcus aureus (S. aureus) is an opportunistic pathogen and a predominant cause of life-threatening nosocomial infections. Drug resistance in S. aureus is attributed to production of biofilm, which is controlled largely by bacterial quorum sensing (QS) systems. Methodology: In vitro analysis of biofilm inhibition assay was performed using crystal violet staining assay, swarming motility, light microscopy and growth curve analyses. Identification of the major constituents of I. verum fruit extract was performed by GC-MS. Ligand-protein interaction was analyzed by molecular docking investigations. Results: The methanol extract of I. verum inhibited the growth of MRSA at the concentration of 4.8 mg/ml. At the sub-inhibitory concentration (2.4mg/ml), the extract showed significant reduction in biofilmogenesis. Light microscopy analysis confirmed the antibiofilm activity as well as the efficacy in disturbing biofilm architecture. A reduced swarming motility was observed at the lowest concentration of 2.4mg/ml. GC-MS analysis revealed anethol (AL) as the major constituent. The molecular docking analysis attributes the antibiofilm activity to an active ligand AL, which strongly interacted with the active site residues of AgrA and SarA proteins of S. aureus. Conclusion: We report the activities of I. verum to be immensely interfering with QS system and biofilm formation in MRSA.

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