scholarly journals INHIBITORY EFFECT OF ONION (ALLIUM CEPA LINN) AND SUGAR PASTE MIXTURE ON STAPHYLOCOCCUS AUREUS AND ESCHERICHIA COLI BY IN VITRO

2019 ◽  
Vol 5 (4) ◽  
pp. 131-137
Author(s):  
Tahiruddin Tahiruddin ◽  
Diah Indriastuti
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Allium Cepa ◽  
Antibacterial Properties ◽  
Inhibition Zone ◽  
Control Group ◽  
Control Group Design ◽  
Onion Extract

Background: Staphylococcus aureus and Escherichia coli are infection agents. The onion is known to have antibacterial properties. Meanwhile, sugar paste is effective to inhibit bacterial growth.Objective: This study aimed to identify the antibacterial activity of onion extract (Allium cepa Linn) and sugar paste mixture on staphylococcus aureus and Escherichia coli by in vitro.Methods: We used a post-test-only control group design with a completely random design. The onion extract with sugar paste mixture was divided into 4 concentrations; 25%, 50%, 75% and 100% with 3 repetitions. The data were analyzed by measuring inhibition zone diameter and tested using the One-Way Analysis of Variance (ANOVA) and Tukey test.Results: Findings showed that on average, the antibacterial activity of onion extract and sugar paste mixture on staphylococcus aureus was 14.57mm, 17.44mm, 18.36mm, and 22.28mm, respectively 25%, 50%, 75%, and 100% concentrations. Meanwhile, on Escherichia coli, it was 17.27mm, 19.67mm, 20.31mm, and 21.62mm.Conclusion: onion extract and sugar paste mixture can inhibit the growth of Staphylococcus aureus and Escherichia coli.

scholarly journals Antibacterial Activity of Cinnamon Extract (Cinnamomum burmannii) against Staphylococcus aureus and Escherichia coli In Vitro

2019 ◽  
Vol 3 (2) ◽  
pp. 19-28
Author(s):  
Nita Parisa ◽  
Rahma Nur Islami ◽  
Ella Amalia ◽  
Mariana Mariana ◽  
Riana Sari Puspita Rasyid
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Control Group ◽  
Antibacterial Compounds ◽  
Cinnamon Extract ◽  
Cinnamomum Burmannii

Abstract   Infectious disease is one of the most common diseases in the world. Staphylococcus aureus and Escherichia coli are two common causes of infection and are resistant to many antibiotics, so the new agents are needed to overcome antibiotic resistance. Cinnamon is often used as a preservative because it has antibacterial activity. Cinnamomum burmannii is kind of native cinnamon from Indonesia. The antimicrobial active compounds cinnamaldehyde and eugenol are the main reasons for its antibacterial activity. This study observed the efficacy of the cinnamon extract (Cinnamomum burmannii) as antibacterial against Staphylococcus aureus and Escherichia coli. An experimental study, in vitro using Post-test Only Control Group Designed, has been done in Microbiology and Biotechnology Laboratory of Medical Faculty of Sriwijaya University. Cinnamon was extracted, then tested for its antibacterial activity using well diffusion and serial dilution to determine diameter of inhibition zone and minimum bactericidal concentration. Phytochemical tests were also conducted to determine the antibacterial compounds of cinnamon extract. Ethanol extract of cinnamon was able to inhibit the growth of Staphylococcus aureus with MBC 5% and inihibitory zone 6,84±0,68 mm and Escherichia coli with MBC 10% and inhibitory zone 5,69±0,69 mm. Cinnamon extract which has the greatest effectiveness is concentration of 40% with inhibition zone 15,69±0,80 mm (Staphylococcus aureus) and 9,63±0,59 mm (Escherichia coli). This ability is due to the antibacterial compounds as evidenced by positive results in various phytochemical tests. Cinnamon extract is effective as antibacterial against Staphylococcus aureus and Escherichia coli in vitro.   Keywords: efficacy, antibacterial, Cinnamomum burmannii, Staphylococcus aureus, Escherichia coli

scholarly journals IN VITRO TESTING OF CLOVE EXTRACTS IN INHIBITING AND KILLING STAPHYLOCOCCUS AUREUS AND ESCHERICHIA COLI

2021 ◽  
Vol 10 (1) ◽  
pp. 16-19
Author(s):  
Nadia Dwi Rahmawati ◽  
Tri Nur Kristina ◽  
Endang Sri Lestari ◽  
Hardian Hardian
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Skin Irritation ◽  
Antibacterial Properties ◽  
Control Group ◽  
Dilution Method ◽  
Control Group Design ◽  
E Coli ◽  
Clove Extract

Background: Diarrhea that could be caused by Staphylococcus aureus and Escherichia coli can be prevented by increasing hand hygiene using alcohol-based hand rub, but frequent use might cause skin irritation. Replacing alcohol with herbs could avoid this side effect. Cloves have been proved to have antibacterial properties. However, most researches used complex extraction methods that might not be applicable on a household scale.Objective: To prove that clove extracts have ability to inhibit and to kill S. aureus and E. coli.Methods: This is an experimental study by using post-test only control group design. Clove extract was obtained with simple aqueous maceration. Samples were Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922 allocated into six groups: clove extracts at concentrations of 12.5%, 25%, 50%, 100% respectively; 70% alcohol as a positive control; and aquadest as a negative control. Each group was given 5 repetitions of intervention. MIC was measured with dilution method, while MBC with streak method. Negative controls were only used to monitor the quality of this work.Results: MIC of S. aureus was started at 50% concentration of clove extract, while MBC of S. aureus was started at 25 % concentration of the same extract. MIC and MBC of E. coli was both started at 25% concentration of clove extract. MIC and MBC of 100% concentration of clove extract were equal with 70% alcohol.Conclusion: Concentration of 100% clove extracts have similar ability with 70% alcohol to inhibit and to kill S. aureus and E. coli. Keywords: Clove Extracts, Escherichia coli, MBC, MIC, Staphylococcus aureus

Polysaccharide-Based Film of Cassia fistula and it's Antibacterial Activity

2014 ◽  
Vol 875-877 ◽  
pp. 87-90 ◽  
Author(s):  
Chutimon Satirapipathkul ◽  
Tanakan Chatdum
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibacterial Properties ◽  
Water Vapor Permeability ◽  
Inhibition Zone ◽  
Vapor Permeability ◽  
Glycerol Concentration ◽  
Bacterial Strains ◽  
Cassia Fistula

The film-forming potential of isolate of seed polysaccharide fromCassia fistulawas investigated. Increasing the glycerol concentration in the film increased elongation at break, film solubility and water vapor permeability but decreased tensile strength (TS). The film impregnated with the acetone extract ofAtractylodes lanceawas assessed for inhibition ofEscherichia coliandStaphylococcus aureus. The obtained results showed that the films exhibited antibacterial activity against both bacterial strains. Disc-diffusion assay revealed that the film resulted in a larger inhibition zone around the film onStaphylococcus aureusthan it did onEscherichia coliat the same extract concentrations (0.1 to 0.9 wt %). It can be seen that the film has satisfactory physical and antibacterial properties.

scholarly journals IN VITRO AND IN SILICO ANTIBACTERIAL ACTIVITY OF 1.5-BIS (3’-ETHOXY-4’-HYDROXYPHENYL)-1-4-PENTADIENE-3-ONE

2018 ◽  
Vol 10 (5) ◽  
pp. 70
Author(s):  
Agus Purwanggana ◽  
Esti Mumpuni ◽  
Esti Mulatsari
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Staphylococcus Epidermidis ◽  
In Silico ◽  
Inhibitory Concentration ◽  
Inhibition Zone ◽  
Antibacterial Study

Objective: The main objective of this research were screened in vitro and in silico of 1,5-bis (3'-ethoxy-4'-hydroxyphenyl)-1,4-pentadiene-3-one as potential antibacterial agents.Methods: The in vitro antibacterial study was carried against Staphylococcus aureus, Staphylococcus epidermidis (gram positive) and Escherichia coli, Salmonella thypi (gram negative) using broth dilution method to determine Minimum Inhibitory Concentration (MIC), disc diffusion method to determine the diameter of inhibition zone. In silico antibacterial study was carried using computational software Protein-Ligand ANT System (PLANTS), computational docking was carried using receptor with Protein Data Bank (PDB) file 3MZD. The structures were optimized prior docking using YASARA, and MarvinSketch. The results of antibacterial testing were compared to two positive control drugs i. e amoxicillin and cefadroxil.Results: In vitro evaluation showed that 1,5-bis (3'-ethoxy-4'-hydroxyphenyl)-1,4-pentadiene-3-one has a better antibacterial activity than amoxicillin and cefadroxil with a Minimum Inhibitory Concentration (MIC) of 0.15 ppm and diameter of inhibition zone of 11.27±0.31, 11.35±0.39, 11.25±0.33, and 11.05±0.45 mm in Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, and Salmonella thypi, respectively. These results in line with in silico evaluation that showed 1,5-bis (3'-ethoxy-4'-hydroxyphenyl)-1,4-pentadiene-3-one has more negative docking score than amoxicillin, cefadroxil, and cloxacillin acyl as a native ligand on the 3MZD receptor.Conclusion: This results obtained in this research work were 1,5-bis (3'-ethoxy-4'-hydroxyphenyl)-1,4-pentadiene-3-one compound potential as an antibacterial agent. 

scholarly journals Relationship Among Antibiotic Residues And Antibacterial Activity Of The Endemic Spurge Honey (Euphorbia Resinifera O. Berg) From Morocco

2020 ◽  
pp. 795
Author(s):  
Rania Benjamaa ◽  
Abdelkarim Moujanni ◽  
Anass Terrab ◽  
Rabiaa Eddoha ◽  
Maryam Benbachir ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Inhibition Zone ◽  
Health Concern ◽  
Resistant Bacteria ◽  
Antibiotic Residues ◽  
Antibiotic Resistant ◽  
Liquid Chromatography Tandem Mass

Antibiotic-resistant bacteria continue to be of major health concern worldwide. In recent years, several reports and scientific articles claim the contamination of honey by antibiotics, detectable concentrations of antibiotic residues in honey are illegal. They, may cause hypersensitivity or resistance to drug therapy in humans, and are perceived by consumers as undesirable. In this sense, the purpose of this work was to examine the antibacterial activity of the Euphorbia resinifera (E. resinifera) honey against Escherichia coli and Staphylococcus aureus in vitro using the well-agar diffusion assay followed by dilution range to obtain more precise minimum inhibitory concentration values. The second aim is to evaluate the presence of antibiotics in honey using a screening test: Evidence InvestigatorTM, an immuno-enzymatic method for detection of 27 antibiotic residues followed by a liquid chromatography-tandem mass spectrometry (LC-MS/MS) for confirmation of suspect samples; in order to assess the relationship between the presence of antibiotic residues and the antibacterial activity of honey. In this study, a total of 37 E. resinifera honey samples were analyzed. The results show that all samples of honey inhibited the growth of bacteria at the dilutions at 50% (v/v); the highest inhibition zone (25.98 ± 0.11 mm) was recorded from sample 5 for Staphylococcus aureus and (13.84 ± 1.10 mm) in sample 17 for Escherichia coli and that 50% (v/v) dilutions showed significant antibacterial effect compared to other dilutions (6.25, 12.5, 25% (v/v)). In all samples, there were no antibiotic residues detected except for one showing the detection of Trimethoprim at 6.48 µg kg-1. Our research is one of the first studies that relate the he relationship between the presence of antibiotic residues and the antibacterial activity of Euphorbia resinifera honey and showed that the antibacterial activity of honey might be due to the high osmotic nature, a low pH, its content of phenolic compounds and hydrogen peroxide and also to its content of methylglyoxal.

scholarly journals EFEK ANTIMIKROBA EKSTRAK RIMPANG KUNYIT (CURCUMA DOMESTICA) TERHADAP ESCHERICHIA COLI SECARA IN VITRO

2017 ◽  
Vol 10 (2) ◽  
pp. 123
Author(s):  
Resa Putra Adiputra ◽  
Irma Suswati ◽  
Fathiyah S
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Post Test

Pengaruh Pemberian Boraks Peroral Sub Akut Terhadap Terjadinya Atrofi Testis Tikus Putih Jantan (Rattus novergicus strain wistar). Latar Belakang: Penggunaan boraks banyak disalahgunakan pada makanan. Boraks merupakan salah satu bahan toksik bagi organ testis sehingga dapat menyebabkan atrofi testis melalui penghambatan spermatogenesis. Tujuan: Membuktikan pengaruh pemberian boraks peroral sub akut terhadap terjadinya atrofi testis tikus putih jantan (Rattus novergicus strain wistar) dengan mengukur diameter testis, berat testis, dan jumlah tubulus seminiferus perlapangpandang. Metode: Eksperimental, The Post Test Only Control Group Design. Sampel yang digunakan 24 ekor dibagi 4 kelompok. Kelompok 1 (kontrol negatif), kelompok 2,3,dan 4 masing-masing dengan dosis 400 mg/kgBB, 500 mg/kgBB, dan 600 mg/kgBB selama 28 hari. Dianalisis dengan oneway ANOVA, uji korelasi, dan uji regresi. Hasil penelitian dan diskusi: Terdapat perbedaan diameter testis dan jumlah tubulus seminiferus masing-masing dengan sig p=0,020 (p<0,05) dan sig p=0,00 (p<0,05), sedangkan pada berat testis tidak terdapat perbedaan dengan sig p=0,744 (p>0,05). Analisis korelasi diameter testis (p=0,001), (r=-0,613), jumlah tubulus (p=0,000), (r=0,828), kenaikan boraks menyebabkan penurunan diameter testis dan peningkatan jumlah tubulus. Analisis regresi R2 diameter testis= 0,376 dan R2 jumlah tubulus=0,685. Pada penelitian ini pengaruh boraks terlihat pada gambaran mikroskopis dibandingkan makroskopis, hal ini disebabkan oleh waktu paparan boraks yang kurang lama. Kesimpulan: Pemberian boraks peroral sub akut berpengaruh terhadap atrofi testis.Kata Kunci: Ekstrak rimpang temulawak, Staphylococcus aureus, KHM (Kadar Hambat Minimum), KBM (Kadar Bunuh Minimum).

scholarly journals Aktivitas Antibakteri Ekstrak Daun Widuri Terhadap Escherichia Coli Dan Staphylococcus Aureus

2020 ◽  
Vol 2 (1) ◽  
pp. 1-9
Author(s):  
Siti Zamilatul Azkiyah
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bacterial Infections ◽  
Leaf Extract ◽  
Antibacterial Properties ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
The Body ◽  
And Staphylococcus Aureus

Antibiotics have a very important role in fighting bacterial infections in the body. However, the results given so far have not been optimal in overcoming the problem of bacterial infection. An alternative solution is to develop and study plants that have medicinal potential, for example thistle leaves (Calotrophis gigantean). The purpose of this study was to determine the antibacterial activity of thistle leaf extract against Escherichia coli and Staphylococcus aureus. This research is a laboratory-scale experimental research that is to test the activity of thistle leaf extract against Escherichia coli and Staphylococcus aureus including sample identification, extraction, activity testing by observing the inhibition zone of the two tested bacteria. Based on the results of the study stated that the ethanol extract of 70% Widuri leaves (Calotrophis gigantea) had antibacterial activity and could inhibit the growth of Escherichia coli and Staphylococcus aureus bacteria. The active compounds that are thought to have antibacterial properties in the ethanol extract of Widuri leaves (Calotrophis gigantea) are flavonoids, alkaloids and terpenoids.   Keywords: Widuri leaves, Escherichia coli, Staphylococcus aureus, Antibacterial ABSTRAK   Pentingnya penanggulangan infeksi bakteri menggunakan antibiotik adalah hal yang lazim dalam terapi farmakologi. Dampak dari pemberian antibiotik masih belum maksimal dalam penanggulangannya terhadap infeksi bakteri. Pemilihan alternatif bahan alam dengan kandungan kimia yang memiliki potensi sebagai antibiotik dapat menjadi pilihan lain dalam mengurangi penggunaan antibiotik sintetik, misalnya tanaman widuri (Calotrophis gigantean). Tujuan dari penelitian ini yaitu untuk mengetahui aktivitas antibakteri ekstrak daun widuri terhadap Escherichia coli dan Staphylococcus aureus. Desain penelitian meliputi identifikasi sampel, ekstraksi, hingga uji aktivitas dengan mengamati zona hambat dari kedua bakteri uji. Hasil penelitian menunjukkan bahwa ekstrak etanol 70% daun Widuri memiliki aktivitas antibakteri dan dapat menghambat pertumbuhan bakteri Escherichia coli dan Staphylococcus aureus. Senyawa aktif yang diduga bersifat antibakteri pada ekstrak etanol daun Widuri adalah senyawa golongan flavonoid, alkaloid dan terpenoid. Kata Kunci: Daun Widuri, Escherichia coli, Staphylococcus aureus, Antibakteri

scholarly journals UJI EFEK ANTIBAKTERI EKSTRAK ETIL ASETAT DAN KLOROFORM MENIRAN (Phyllanthus niruri Linn) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus ATCC 6538 DAN Escherichia coli ATCC 11229 SECARA in vitro

Biomedika ◽  
2012 ◽  
Vol 4 (2) ◽  
Author(s):  
Dwiariawan Tauchid Rahman ◽  
EM Sutrisna ◽  
Anika Candrasari
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Chloroform Extract ◽  
Control Group ◽  
Phyllanthus Niruri ◽  
Control Group Design ◽  
Post Test ◽  
And Staphylococcus Aureus ◽  
Test Control

Meniran is one of traditional plants that contains several compounds such as terpenoid, flavonoid, and alkaloid that role as antibactrial agent. The aims of the research are to determine the antibacteria effect of extract aethyl acetat and chloroform of meniran. This research was conducted as a laboratory experimental by using post test control group design only. The concentration of extract aethyl acetat and chloroform meniran are 5%, 10%, 20%, 40%, and 80%. The result indicate that extract aethyl acetat of meniran have not effect to Escherichia coli. Meanwhile, to Staphyloccus aureus have an effect (p=0,003). The concentration of the extract chloroform meniran are effective to inhibite the growth of Escherichia coli (p=0,029) and Staphylococcus aureus (p=0,004). The extract aethyl acetat of meniran have an antibacteria effect to Staphylococcus aureus but not to Escherichia coli. Whereas, the extract chloroform of meniran have an antibacteria effect to Staphylococcus aureus and Escherichia coli.Keyword : The aethyl acetat and chloroform extract of Meniran (Phyllanthus niruri L.), antibacteria effect, Staphylococcus aureus and Escherichia coli

scholarly journals Perbedaan Daya Antibakteri Antara Kombinasi Calcium Hydroxide-Based Sealer-Amoxicillin Dan Resin-Based Sealer-Amoxicillin Terhadap Bakteri Enterococcus Faecalis

2019 ◽  
Vol 8 (1) ◽  
pp. 1
Author(s):  
Asarizka Bena ◽  
Agus Subiwahjudi ◽  
Setyabudi Setyabudi
Keyword(s):  
Antibacterial Activity ◽  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
Antibacterial Properties ◽  
Inhibition Zone ◽  
Control Group ◽  
Control Group Design ◽  
Root Canal Obturation ◽  
Agar Diffusion Test

Background. About 24-77% of root canal treatment failure cases are caused by infection of Enterococcus faecalis due to the resistance factor and virulence of these bacteria. Calcium hydroxide-based sealer and resin-based sealer are two types of sealer often used for root canal obturation due to their antibacterial properties. But the antibacterial properties owned by calcium hydroxide-based sealer has no effect on Enterococcus faecalis. Therefore, the adjunct of local antimicrobial such as amoxicillin is needed to increase antibacterial activities of root canal obturation materials (sealers) especially for calcium hydroxide-based sealer. Purpose. The aim of this study was to compare the effectiveness of antibacterial activity of calcium hydroxide-based sealer-amoxicillin and resin-based sealer-amoxicillin against Enterococcus faecalis. Methods. This study is an experimental laboratory with post test only control group design using Enterococcus faecalis ATCC 29212. Agar diffusion test was used to check the antimicrobial activity of calcium hydroxide-based sealer-amoxicillin and resin-based sealer-amoxicillin. by measuring the inhibition zone diameter of each treatment. Results. Resin-based sealer-amoxicillin’s diameter of zone inhibition is 37,3 mm and calcium hydroxide-based sealer-amoxicillin’s diameter is 18,7 mm. Conclusion. Antibacterial activity of resin-based sealer-amoxicillin is significantly greater than calcium hydroxide-based sealer-amoxicillin.

scholarly journals Comparison of Antibacterial Activity of Sambiloto (Andrographis paniculata) Ethanol and Water Stem Extract Against Methicillin-Resistant Staphylococcus aureus (MRSA) ATCC 3351 In Vitro

2020 ◽  
Vol 5 (2) ◽  
pp. 69-76
Author(s):  
Ni Wayan Sinta Devi Arini ◽  
Bagus Komang Satriyasa ◽  
I Made Jawi ◽  
Agung Wiwiek Indrayani
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Andrographis Paniculata ◽  
Control Group ◽  
Inhibitory Zone ◽  
Control Group Design ◽  
Methicillin Resistant ◽  
Stem Extract

Background: Methicillin-resistant Staphylococcus aureus (MRSA) is penicillin and cephalosporine resistant Staphylococcus aureus which is a major cause of nosocomial infection. Various studies have been conducted for resistant cases, especially herbs which have antibacterial activity. Sambiloto (Andrographis paniculata) is an example of herb which has antibacterial activity due to the presence of andrographolide. Andrographolide soluble in ethanol and poorly in water, while extraction with alcohol causes andrographolide’s degradation. This study aimed to investigate the antimicrobial activity of ethanol and water stem extract and compare them against MRSA ATCC 3351 in vitro.Methods: This is an experimental study with a post-test only control group design and conducted by disc diffusion technique to obtain an inhibition zone against MRSA.Result: The result of this study showed the mean inhibitory zone of ethanol stem extract was 5,87mm, 6,77mm, 7,87mm respectively for 25%, 50%, and 100% concentrations, while 1% concentration did not show antibacterial activity. Water stem extract at 1%, 25%, 50%, and 100% concentrations showed mean inhibitory zone was at 4,57mm, 7,17mm, 8,67mm, and 9,67mm respectively.Conclusion: Ethanol and water stem extract showed antibacterial activity against MRSA. The independent sample t-test didn’t show a difference between ethanol and water stem extract of Andrographis paniculata toward MRSA (p>0,05).

Sign in / Sign up

Export Citation Format

Share Document