scholarly journals Study the Haematological parameters and Oxidative stress before and after disbudding in Calves

2021 ◽  
pp. 62-69
Author(s):  
Shiv Swaroop ◽  
Pramanik PS ◽  
Singh KD ◽  
Subodh Kumar ◽  
Verma AK
Keyword(s):  
Oxidative Stress ◽  
Animal Husbandry ◽  
Lipid Peroxides ◽  
Haematological Parameters ◽  
Veterinary Science ◽  
Treatment Groups ◽  
Significant Difference ◽  
Haematological Profile ◽  
Before And After ◽  
And Oxidative Stress

The aim of the present investigation was to examine the haematological profile and oxidative stress before and after disbudding in calves. The study was carried out at Livestock Farm Complex, College of Veterinary Science and Animal Husbandry, ANDUAT, Kumarganj, Ayodhya and nearby villages. Twenty four cattle calves were utilized to study haematological parameters that are Haemoglobin (Hb (gm/dl), Neutrophil (%), Basophil (%), PCV (%), Lymphocyte (%), Monocyte (%), TLC (103/ µl), Eosinophil (%), N/L ratio) and oxidative stress before and after disbudding in to four groups viz. control (T0), lignocaine (TL), tremadol (TT) and lignocaine+tremadol (TL-T). After medication calves were disbudded by hot iron method. The blood samples were collected for haematological parameters & for estimation of LPO (lipid peroxides) as per method described by [1] just before (0h) and after disbudding on 6h, 24h and 72h. The results showed that, at 24h, T0 group showed significantly lowest Hb (10.66±0.49%) which was statistically similar to TT and TL-T groups. At 0h, 6h and 72h means of PCV% were significantly different among treatment groups. The lowest lymphocytes (64.00±1.00) was observed in TT group at 6h. The T0 group showed significantly lowest basophil (0.00±0.00) which was statistically similar to TT and TL-T groups but significantly different from TL group at 6h. At the same interval, T0 group showed significantly lowest monocytes (4.66±0.61) which was significantly different with TL, TT and TL-T groups. At 6h, T0 group showed significantly lowest N/ L ratio (0.15±0.01) which was statistically similar to TT and TL-T groups but significantly different from TL group. At 0h, 6h and 24h LPO was significantly different among groups. At 0h, T0 group showed significantly highest (106.59±0.63) which was significantly different from TL, TT and TL-T groups. It may be concluded that no significant difference was observed in eosinophil%, TLC, neutrophil% and monocyte% counts between control and treatment groups. However, Hb%, basophil%, PCV% and lymphocyte% were significantly different between control and treatment groups. N/L ratio was significant only at 6h. Most of the cases TL-T group showed comparatively better performance. At 0h, 6h and 24h LPO was significantly different among groups. At 0h, T0 group showed significantly highest (106.59±0.63) which was significantly different from TL, TT and TL-T groups.

scholarly journals Haematological and biochemical profiles of Gallus indigenous, exotic and hybrid chicken breeds (Gallus domesticus l.) from Rajshahi, Bangladesh

2015 ◽  
Vol 41 (2) ◽  
pp. 135-144 ◽  
Author(s):  
Ripon Kumar Dutta ◽  
M Saiful Islam ◽  
Md Ashraful Kabir
Keyword(s):  
Rhode Island ◽  
Biochemical Parameters ◽  
Gallus Domesticus ◽  
Haematological Parameters ◽  
Breeding Strategies ◽  
Significant Difference ◽  
Haematological Profile ◽  
Biochemical Profiles ◽  
Chicken Breeds ◽  
Differential Counts

A comparative account of haemato-biochemical profiles of an indigenus (IND) and five chicken breeds: viz. Cobb 500 (COB), cockerel (COC), Fayoumi (FAY), Rhode Island Red (RIR), and Sonali (SON, derived from RIR cock × FAY hen), has been presented. The haematological profile included total counts (TC) of RBC, WBC, platelets, haemoglobin (Hb%) and ESR and differential counts (DC) of WBC included neutrophils, eosinophils, lymphocytes, monocytes and basophils. All the haematological parameters except WBC, ESR and basophils showed significant difference among the breeds (P < 0.01). Vital biochemical parameters from blood sera such as calcium, cholesterol, creatinine, glucose and urea also showed significant difference among the chicken breeds (P < 0.05). The haematobiochemical parameters, however, were not significantly correlated (P > 0.05) with each other among the chicken breeds. Except for cholesterol vs. urea in IND, none of the correlations tested for haemato-biochemical profiles of the experimental chickens was found significant. Relevance of this study in relation to health, clinico-pathology and improved breeding strategies of the poultry species in the country has been discussed.Bangladesh J. Zool. 41(2): 135-144, 2013

scholarly journals The Effect of Herniarin on Spatial Working Memory, Pain Threshold, and Oxidative Stress in Ischemic Hypoperfusion Model in Rats

2021 ◽  
Vol 7 (1) ◽  
pp. 42-50
Author(s):  
Zahra Nazari Barchestani ◽  
◽  
Maryam Rafieirad ◽  
Keyword(s):  
Oxidative Stress ◽  
Pain Threshold ◽  
Male Rats ◽  
Control Group ◽  
Tail Flick ◽  
Pain Thresholds ◽  
Ischemic Group ◽  
Significant Difference ◽  
The Brain ◽  
And Oxidative Stress

Background: Ischemia causes severe neuronal damage and induces oxidative stress, memory impairment, and reduces pain threshold. Herniarin is a powerful antioxidant. Objectives: This study aimed to evaluate the effect of herniarin on memory, pain, and oxidative stress in an ischemia model in male rats. Materials & Methods: In this study, 50 male rats were divided into 5 groups of control, sham, ischemic, and two other ischemic groups, which received herniarin at doses of 150 and 300 mg/kg by gavage for 14 days. Behavioral tests were performed by shuttle box, and Y-maze and pain tests were performed by Tail-Flick test. Then, the rats’ brains were extracted to evaluate lipid peroxidation and measure the levels of thiol and Glutathione Peroxidase (GPX) in the hippocampus and striatum tissues. The results were expressed as Mean±SEM and then analyzed using suitable statistical methods of ANOVA and least significant difference post-hoc test in SPSS V. 20. Results: Herniarin significantly increased the avoidance memory, spatial memory, and pain thresholds of ischemic rats at different concentrations (P<0.001). Besides, the amount of malondialdehyde (MDA) and thiol in the ischemic group increased significantly in comparison to the control group (P<0.001). Also, in the ischemic group, GPX (P<0.001) significantly decreased. Decreased MDA (P<0.001) and thiol (P<0.001) and increased GPX levels were observed with herniarin administration (P<0.01). Conclusion: According to this study’s results, herniarin can remove free radicals and oxidant substances from the brain. Thus, it improves memory and pain thresholds in the brain hypoperfusion ischemia model.

scholarly journals Malondialdehyde Levels in Preeclampsia before and after Delivery

1970 ◽  
pp. 143
Author(s):  
Harold Rumopa ◽  
Freddy W. Wagey ◽  
Eddy Suparman
Keyword(s):  
Oxidative Stress ◽  
Plasma Levels ◽  
Cross Sectional Study ◽  
Hplc Method ◽  
Wilcoxon Test ◽  
Sectional Study ◽  
Cross Sectional ◽  
Average Value ◽  
Significant Difference ◽  
Before And After

  Objective: Determine differences plasma levels MDA in preeclampsiabefore and 2 hours after delivery.   Methods: This was an analytic cross-sectional study. Subjectconsists of 23 pregnancies with preeclampsia, where 23 bloodsamples taken before delivery and 23 were taken 2 hours afterdelivery. This study was conducted from August 2016 untilDecember 2016 at Department of Obstetrics and GynecologyFaculty of Medicine Universitas Sam Ratulangi / Prof. Dr. R. D.Kandou Hospital Manado and satellite hospital. Samples weretaken from plasma and analysed using HPLC method at Prodiaclinical laboratory.   Results: In patients with severe preeclampsia before deliverywe found average value (1.4796  0.40819 nmol/ml), minimumvalue (1.03 nmol/ml) and maximal value (2.77 nmol/ml)and 2 hours after delivery with average value (1.2470 0.34324 nmol/ml), minimum value (0.91 nmol/ml), and maximumvalue (2.47 nmol/ml). by using Wilcoxon test, we foundthere were significant differences in plasma levels of MDA (p =0.000).   Conclusion: This significant difference suggests that decreasedplasma levels of MDA 2 hours after delivery and gives the sense thatthere is a relationship between oxidative stress of cells with severepreeclampsia before and shortly after delivery, that MDA is an indicatorof oxidative stress.   Keywords: malondialdehyde, oxidative stress, peroxidation lipid,preeclampsia

scholarly journals Effects of Monomeric and Oligomeric Flavanols on Kidney Function, Inflammation and Oxidative Stress in Runners: A Randomized Double-Blind Pilot Study

Nutrients ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 1634 ◽  
Author(s):  
Khrystyna O. Semen ◽  
Antje R. Weseler ◽  
Marcel J. W. Janssen ◽  
Marie-José Drittij-Reijnders ◽  
Jos L. M. L. le Noble ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Kidney Function ◽  
Randomized Study ◽  
Kidney Injury ◽  
Strenuous Exercise ◽  
Vitis Vinifera L ◽  
Grape Seeds ◽  
Double Blind ◽  
Before And After ◽  
And Oxidative Stress

Nonsteroidal anti-inflammatory drugs are frequently used by athletes in order to prevent musculoskeletal pain and improve performance. In combination with strenuous exercise, they can contribute to a reduction of renal blood flow and promote development of kidney damage. We aimed to investigate whether monomeric and oligomeric flavanols (MOF) could reduce the severity of kidney injuries associated with the intake of 400-mg ibuprofen followed by the completion of a half-marathon in recreational athletes. In this double-blind, randomized study, the original MOF blend of extracts from grape seeds (Vitis vinifera L.) and pine bark (Pinus pinaster L.) or placebo were taken for 14 days preceding the ibuprofen/half-marathon. Urine samples were collected before and after the ibuprofen/half-marathon, and biomarkers of kidney injury, inflammation and oxidative stress were assessed. Intake of MOF significantly reduced the incidence of post-race hematuria (p = 0.0004) and lowered concentrations of interleukin (IL)-6 in the urine (p = 0.032). Urinary neutrophil-associated lipocalin, creatine, albumin, IL-8 and malondialdehyde tended to decrease. The supplementation with MOF in recreational runners appears to safely preserve kidney function, reduce inflammation and promote antioxidant defense during strenuous exercise and intake of a single dose of ibuprofen.

scholarly journals Role of Anti-oxidants in the Treatment of Vitiligo

2019 ◽  
Vol 17 (1) ◽  
pp. 49-57
Author(s):  
Amit Kumar ◽  
Sudha Agrawal ◽  
Tapan Kumar Dhali ◽  
Shankar Kumr Majhi
Keyword(s):  
Oxidative Stress ◽  
Placebo Group ◽  
Vitamin E ◽  
Primary Outcome ◽  
Antioxidant Supplementation ◽  
Significant Difference ◽  
Anti Oxidant ◽  
And Oxidative Stress

Introduction: The role of free radicals and oxidative damage in the pathophysiology of vitiligo has been documented in recent studies. Antioxidant supplementation has been reported to be useful in the treatment of vitiligo. Objective: To evaluate the role of oral antioxidants supplementation therapy in the treatment of vitiligo by assessing the onset of repigmentation and oxidative stress. Materials and Methods: A total of 80 cases of vitiligo randomized into two groups: antioxidant and placebo comprising 40 patients each and were followed up for 8 weeks for the assessment of onset of repigmentation of vitiliginous lesions as primary outcome.  The activities of Malondialdehyde (MDA), Vitamin C, and Vitamin E in serum and of Catalase (CAT) in erythrocytes of patients at baseline and at end of eight weeks were also assessed by using the spectrophotometric assay. Results: The onset of repigmentation was noted significantly earlier among the anti-oxidant group as compared to the placebo group (p=0.015). At the baseline, between the two groups, no significant difference was found in the different biochemical parameters. However, at the end of 2 months the level of MDA (p<0.001) was found to be significantly lower and that of Vitamin E (p<0.001) and CAT (p=0.005) was significantly higher among the anti-oxidants group as compared to the placebo group. Conclusion: Antioxidant supplementation carried a better response in terms of early onset of repigmentation and significant decrease in the oxidative stress, in the short follow up of two months.

Evaluation of oxidative stress and antioxidant parameters in children with carbon monoxide poisoning

2019 ◽  
Vol 38 (11) ◽  
pp. 1235-1243
Author(s):  
O Teksam ◽  
S Sabuncuoğlu ◽  
G Girgin ◽  
H Özgüneş
Keyword(s):  
Oxidative Stress ◽  
Carbon Monoxide ◽  
Carbon Monoxide Poisoning ◽  
Venous Blood ◽  
Treatment Modalities ◽  
Control Group ◽  
Co Poisoning ◽  
Erythrocyte Catalase ◽  
Significant Difference ◽  
Before And After

Objective: In this study, we aimed to investigate oxidative stress and antioxidant parameter levels in patients with carbon monoxide (CO) poisoning. Methods: The study was conducted prospectively between March 1, 2015 and April 30, 2016 in the pediatric emergency department. Eligible patients included children aged 0–18 years old with a diagnosis of CO poisoning. To determination of oxidative stress and antioxidant parameter levels, venous blood with heparinized and urine samples were drawn during the admission and after normobaric oxygen (NBO) and hyperbaric oxygen (HBO) treatment. Results: Forty-seven children with CO poisoning for study group and 29 patients as control group were included to the study. Sixteen patients treated with HBO. Basal plasma malondialdehyde levels were found to be significantly higher in the CO poisoning group when compared with the control group ( p = 0.019). There is no significant difference in oxidative stress and antioxidant parameter levels except erythrocyte catalase enzyme levels in patients treated with NBO when comparing before and after NBO treatment ( p > 0.05). Decreasing of basal erythrocyte catalase enzyme levels were found statistically significant after NBO treatment ( p = 0.04). There was no significant difference in oxidative stress and antioxidant parameter levels in patients treated with HBO before and after therapy ( p > 0.05). Conclusions: CO poisoning is associated with increased lipid peroxidation in children immediately after the poisoning. However, both treatment modalities including NBO or HBO do not have a significant effect on oxidative stress or antioxidant parameter levels.

scholarly journals Effects of menopause and tibolone on antioxidants in postmenopausal women

2005 ◽  
Vol 42 (3) ◽  
pp. 220-223 ◽  
Author(s):  
Perin Vural ◽  
Cemil Akgül ◽  
Mukaddes Canbaz
Keyword(s):  
Oxidative Stress ◽  
Ascorbic Acid ◽  
Postmenopausal Women ◽  
Plasma Concentrations ◽  
Lipid Peroxide ◽  
Lipid Peroxides ◽  
Thiol Groups ◽  
Plasma Ascorbic Acid ◽  
Total Thiol ◽  
Significant Difference

Background: Oxidative stress has been implicated in the pathogenesis of ageing and menopause, and can arise through the increased production of lipid peroxides and/or a deficiency of antioxidant defence. Aim: To investigate the effects of the menopause and tibolone treatment (2.5 mg/day for six months) on plasma antioxidants and lipid peroxidation. Methods: Plasma concentrations of ascorbic acid, α-tocopherol, total thiol groups, caeruloplasmin, erythrocyte glutathione (GSH) and malondialdehyde (MDA) were measured in 24 postmenopausal and 24 premenopausal healthy women. Results: Data analysis indicates a significant decrease in plasma ascorbic acid, α-tocopherol, total thiol groups, caeruloplasmin, erythrocyte GSH and a significant increase in lipid peroxides (expressed as MDA concentrations) in postmenopausal women. There was no significant difference between control and study groups in the mean plasma caeruloplasmin concentrations. It was found that there is a significant increase in α-tocopherol and significant decrease in lipid peroxide concentrations in postmenopausal after tibolone treatment. Conclusions: The menopause is associated with an increase in oxidative stress and a decrease of some antioxidants, such as ascorbic acid, α-tocopherol, total thiols and erythrocyte GSH. Tibolone treatment leads to a decrease in concentrations of plasma lipid peroxide, probably by stimulating direct and indirect mechanisms of tocopherol regeneration and increasing plasma concentrations of vitamin E. However, due to the relatively small numbers involved this study can be regarded as a pilot. Further studies performed on a larger scale are necessary to establish the exact mechanisms of tibolone in inhibiting oxidative stress in postmenopausal women.

Effects of xylazine-ketamine, xylazine-propofol and xylazine-ketamine-propofol administration on free radical generation and blood gases in sheep

2018 ◽  
Author(s):  
E. Gokalp ◽  
S. Gurgoze ◽  
S. Altan
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Status ◽  
Blood Gases ◽  
Control Group ◽  
Intravenous Route ◽  
Blood Gas ◽  
Treatment Groups ◽  
Glucose Levels ◽  
Significant Difference ◽  
Radical Generation

This study was aimed at investigating the effects of xylazine-ketamine, xylazine-propofol and xylazine-ketamine-propofol combinations on oxidative stress, antioxidant capacity and blood gases in sheep. Excluding the control animals, the sheep included in Groups 1, 2 and 3 were administered with combinations of xylazine-ketamine, xylazine-propofol and xylazine-ketamine-propofol, respectively, by intravenous route. The comparison of the three treatment groups with the control group showed that no significant difference existed for TAS, TOS, MDA and CAT levels. The evaluation of blood gas and electrolyte levels demonstrated a significant decrease in PvO2, cSO2, Na, and Ca levels, and a significant increase in glucose levels. In result, this study showed that the three anaesthetic combinations tested did not have any adverse effect on the oxidant/antioxidant status, but caused significant alterations in blood gas levels.

scholarly journals Homocysteine Exposure Impairs Myocardial Resistance to Ischaemia Reperfusion and Oxidative Stress

2015 ◽  
Vol 37 (6) ◽  
pp. 2265-2274 ◽  
Author(s):  
Amer Almashhadany ◽  
Dareuosh Shackebaei ◽  
Thomas Van der Touw ◽  
Graham L. Jones ◽  
M.-Saadeh Suleiman ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Calcium Transient ◽  
Rate Pressure Product ◽  
Rat Cardiomyocytes ◽  
Ischaemia Reperfusion ◽  
Rat Hearts ◽  
Before And After ◽  
Cardiomyocyte Contractility ◽  
Perfused Rat Hearts ◽  
And Oxidative Stress

Background/Aims: Hyperhomocysteinaemia is recognised as a strong independent risk factor for developing cardiovascular disease. This study investigated how an acute homocysteine dose affected cardiac performance during ischaemia reperfusion and cardiomyocyte contractility and morphology under normal conditions and during oxidative stress. Methods: Cardiac function was measured in isolated and perfused rat hearts before and after 40 minutes' global normothermic ischaemia. Where used, 0.1 mM L-homocysteine was present prior to, and throughout ischaemia, before wash out after 10 minutes' reperfusion. Calcium transients under normal conditions and changes in contractile synchronicity during oxidative stress (exposure to 0.2 mM H2O2) were measured in freshly isolated rat cardiomyocytes incubated for 60 minutes ± 0.1 mM L-homocysteine. Results: During ischaemia reperfusion 0.1 mM L-homocysteine significantly reduced the rate pressure product during reperfusion (10,038 ± 749 vs. 5955 ± 567 mmHg bpm, p < 0.001), but did not affect time to ischaemic contracture. Incubation of freshly isolated cardiomyocytes with 0.1 mM L-homocysteine significantly decreased the amplitude of the calcium transient and slowed the time to half relaxation. Conclusions: These findings suggest that homocysteine exposure affected myocardial recovery from ischaemia and contractile homeostasis although the exact mechanisms for these changes remain to be determined.

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