Antimicrobial resistance and serotypes of Salmonella isolated from food products of animal origin

2021 ◽  
Vol 1 (3) ◽  
pp. 20-26
Author(s):  
S. Yu. Karabanov ◽  
◽  
D. A. Makarov ◽  
O. V. Karabanova ◽  
O. E. Badmaev ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Data Analysis ◽  
Antimicrobial Resistance ◽  
Russian Federation ◽  
Multiple Drug Resistance ◽  
Food Products ◽  
Animal Origin ◽  
Multiple Drug ◽  
The Russian Federation ◽  
Animal Food Products

Salmonella isolates (n=124) were taken from animal food products in different regions of the Russian Federation and investigated. Serogroup was determined, as well as sensitivity to antibacterial drugs. High levels of resistance to fluoroquinolones were revealed (70,97% of isolates resistant to ciprofloxacin). Multiple drug resistance was determined (16,1% of multiresistant isolates). Data analysis was performed using the AMRcloud online platform.

scholarly journals Antimicrobial Resistance Profiling of Biofilm Forming Non Typhoidal Salmonella enterica Isolates from Poultry and Its Associated Food Products from Pakistan

Antibiotics ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 785
Author(s):  
Abubakar Siddique ◽  
Sara Azim ◽  
Amjad Ali ◽  
Saadia Andleeb ◽  
Aitezaz Ahsan ◽  
...  
Keyword(s):  
Public Health ◽  
Antimicrobial Resistance ◽  
Biofilm Formation ◽  
Salmonella Enteritidis ◽  
Multiple Drug Resistance ◽  
Food Products ◽  
Health Concern ◽  
Multiple Drug ◽  
Zoonotic Potential ◽  
Salmonella Spp

Salmonellosis caused by non-typhoidal Salmonellaenterica from poultry products is a major public health concern worldwide. This study aimed at estimating the pathogenicity and antimicrobial resistance in S. enterica isolates obtained from poultry birds and their food products from different areas of Pakistan. In total, 95/370 (25.67%) samples from poultry droppings, organs, eggs, and meat were positive for Salmonella. The isolates were further identified through multiplex PCR (mPCR) as Salmonella Typhimurium 14 (14.7%), Salmonella Enteritidis 12 (12.6%), and other Salmonella spp. 69 (72.6%). The phenotypic virulence properties of 95 Salmonella isolates exhibited swimming and/or swarming motility 95 (100%), DNA degrading activity 93 (97.8%), hemolytic activity 92 (96.8%), lipase activity 87 (91.6%), and protease activity 86 (90.5%). The sopE virulence gene known for conferring zoonotic potential was detected in S. Typhimurium (92.8%), S. Enteritidis (100%), and other Salmonella spp. (69.5%). The isolates were further tested against 23 antibiotics (from 10 different antimicrobial groups) and were found resistant against fifteen to twenty-one antibiotics. All isolates showed multiple drug resistance and were found to exhibit a high multiple antibiotic-resistant (MAR) index of 0.62 to 0.91. The strong biofilm formation at 37 °C reflected their potential adherence to intestinal surfaces. There was a significant correlation between antimicrobial resistance and the biofilm formation potential of isolates. The resistance determinant genes found among the isolated strains were blaTEM-1 (59.3%), blaOxA-1 (18%), blaPSE-1 (9.5%), blaCMY-2 (43%), and ampC (8.3%). The detection of zoonotic potential MDR Salmonella in poultry and its associated food products carrying cephalosporin and quinolone resistance genes presents a major threat to the poultry industry and public health.

scholarly journals Nocardiosis: an overview and additional report of 28 cases in cattle and dogs

2008 ◽  
Vol 50 (3) ◽  
pp. 177-185 ◽  
Author(s):  
Márcio Garcia Ribeiro ◽  
Tatiana Salerno ◽  
Ana Luiza de Mattos-Guaraldi ◽  
Thereza Cristina Ferreira Camello ◽  
Hélio Langoni ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Multiple Drug Resistance ◽  
Bovine Milk ◽  
Bovine Mastitis ◽  
Animal Origin ◽  
Nocardia Asteroides ◽  
Multiple Drug ◽  
Type Iv ◽  
Type V ◽  
Epidemiological Characteristics

Phenotypic characteristics, antimicrobial susceptibility profile, and clinical-epidemiological features of 28 Nocardia strains isolated from 19 cases of bovine mastitis, eight cutaneous-subcutaneous lesions and one case of pneumonia in dogs were evaluated. Microbiological, biochemical, cytological and scanning electron microscopy methods were used in diagnosis. Nocardia asteroides type IV, Nocardia otitidiscaviarum,Nocardia nova (type III) and Nocardia farcinica (type V) were isolated from bovine milk, bronchial lavage and/or cutaneous-subcutaneous abscesses in dogs. Nocardial bovine mastitis was diagnosed predominantly in clinical cases, in dairy herds with poor environmental hygienic conditions between milking and inappropriate intramammary therapy. Canine nocardiosis was observed commonly in animals co-infected with distemper virus. Sulphamethoxazole-trimethoprim (92.8%), amikacin (92.8%) and ceftiofur (92.8%) were the most effective drugs in 28 isolates. Multiple drug resistance to three or more and five or more antimicrobials was observed in ten (35.7%) and three (10.7%) strains, respectively, predominantly with use of cloxaxillin, cefoperazone and ampicillin. The species (type) classification, clinical-epidemiological characteristics, diagnosis, multiple-drug resistance and public health considerations in Nocardia strains isolated from cattle and dogs in Brazil are discussed, with special reference to report of bovine mastitis by N. otitidiscaviarum by first time in Brazil and the similarity between Nocardia species isolated from human and animal origin.

scholarly journals Prevalence of virulence and antimicrobial resistance genes in Salmonella spp. isolated from commercial chickens and human clinical isolates from South Africa and Brazil

2016 ◽  
Vol 83 (1) ◽  
Author(s):  
Oliver T. Zishiri ◽  
Nelisiwe Mkhize ◽  
Samson Mukaratirwa
Keyword(s):  
Drug Resistance ◽  
Antimicrobial Resistance ◽  
South African ◽  
Resistance Genes ◽  
Broiler Chickens ◽  
Antimicrobial Agents ◽  
Multiple Drug Resistance ◽  
Multiple Drug ◽  
Poultry Production ◽  
Antimicrobial Resistance Genes

Salmonellosis is a significant public health concern around the world. The injudicious use of antimicrobial agents in poultry production for treatment, growth promotion and prophylaxis has resulted in the emergence of drug resistant strains of Salmonella. The current study was conducted to investigate the prevalence of virulence and antimicrobial resistance genes from Salmonella isolated from South African and Brazilian broiler chickens as well as human clinical isolates. Out of a total of 200 chicken samples that were collected from South Africa 102 (51%) tested positive for Salmonella using the InvA gene. Of the overall 146 Salmonella positive samples that were screened for the iroB gene most of them were confirmed to be Salmonella enterica with the following prevalence rates: 85% of human clinical samples, 68.6% of South African chicken isolates and 70.8% of Brazilian chicken samples. All Salmonella isolates obtained were subjected to antimicrobial susceptibility testing with 10 antibiotics. Salmonella isolates from South African chickens exhibited resistance to almost all antimicrobial agents used, such as tetracycline (93%), trimethoprim-sulfamthoxazole (84%), trimethoprim (78.4%), kanamycin (74%), gentamicin (48%), ampicillin (47%), amoxicillin (31%), chloramphenicol (31%), erythromycin (18%) and streptomycin (12%). All samples were further subjected to PCR in order to screen some common antimicrobial and virulence genes of interest namely spiC, pipD, misL, orfL, pse-1, tet A, tet B, ant (3")-la, sul 1 and sul. All Salmonella positive isolates exhibited resistance to at least one antimicrobial agent; however, antimicrobial resistance patterns demonstrated that multiple drug resistance was prevalent. The findings provide evidence that broiler chickens are colonised by pathogenic Salmonella harbouring antimicrobial resistance genes. Therefore, it is evident that there is a need for prudent use of antimicrobial agents in poultry production systems in order to mitigate the proliferation of multiple drug resistance across species.Keywords: Salmonella; antimicrobial resistance; chicken; human; susceptibility; virulence gene

scholarly journals Antimicrobial resistance and the ecology ofEscherichia coliplasmids

1984 ◽  
Vol 93 (2) ◽  
pp. 181-188 ◽  
Author(s):  
D. J. Platt ◽  
J. S. Sommerville ◽  
C. A. Kraft ◽  
M. C. Timbury
Keyword(s):  
Escherichia Coli ◽  
Drug Resistance ◽  
Antimicrobial Resistance ◽  
Multiple Drug Resistance ◽  
Clinical Isolates ◽  
Multiple Drug ◽  
Drug Resistant ◽  
E Coli ◽  
R Plasmids ◽  
High Level

SummaryFour hundred and seven clinical isolates ofEscherichia coliwere examined for the presence of plasmids. These isolates comprised 189 which were collected irrespective of antimicrobial resistance (VP) and 218 which were collected on the basis of high-level trimethoprim resistance (TPR). The VP isolates were divided into drug sensitive (VPS) and drug-resistant (VPR) subpopulations.Plasmids were detected in 88% of VP isolates (81% of VPS and 94% of VPR) and 98% of TPR isolates. The distribution of plasmids in both groups and subpopulations was very similar. However, there were small but statistically significant differences between the plasmid distributions. These showed that more isolates in the resistant groups harboured plasmids than in the sensitive subpopulation (VPS) and that the number of plasmids carried by resistant isolates was greater. Multiple drug resistance was significantly more common among TPR isolates than the VPR subpopulation and this was paralleled by increased numbers of plasmids.Fifty-eight per cent of VPR and 57% of TPR isolates transferred antimicrobial resistance and plasmids toE. coliK12. Of the R+isolates, 60% carried small plasmids (MW < 20Md) and 52% of these co-transferred with R-plasmids. These results are discussed.

scholarly journals ANTIBIOTIC RESISTANCE OF VIBRIO CHOLERAE O1 EL TOR ISOLATED ON THE TERRITORY OF THE RUSSIAN FEDERATION IN 2006-2015

2017 ◽  
Vol 22 (1) ◽  
pp. 25-30
Author(s):  
L. A Egiazaryan ◽  
N. A Selyanskaya ◽  
I. B Zakharova ◽  
M. V Podshivalova ◽  
E. A Bereznyak ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Vibrio Cholerae ◽  
Russian Federation ◽  
Molecular Mechanisms ◽  
Multiple Drug Resistance ◽  
Antibiotic Resistance Genes ◽  
Multiple Drug ◽  
Environmental Objects ◽  
The Russian Federation ◽  
El Tor

Aim of the study. The analysis of antibiotic resistance and molecular mechanisms of the persistence of Vibrio cholerae О1 El Tor strains, isolated from patients and environmental objects on the territory of the Russian Federation in 2006-2015. Material and Methods. The susceptibility of 34 V. cholerae El Tor strains to 13 antibacterial drugs was determined by the method of serial dilutions in a rich culture medium (Instructional Guidelines (МUК) 4.2.2495-09). Results. The strains showed resistance to 1-5 antibiotics (levomicetin, streptomycin, nalidixic acid, furazolidone, trimethoprim/sulfamethoxazole) and harbored SXT-element with antibiotic resistance genes. The emergence of quinolone resistance in V. cholerae O1 El Tor strains with accompanying multiple drug resistance limits the choice for drugs for etiotropic therapy and aggravates the possibility of unfavourable cholera outcome.

scholarly journals Zmiany w polskim handlu zagranicznym produktami rolno spożywczymi z Federacją Rosyjską w latach 2006-2015

2018 ◽  
Vol 18(33) (1) ◽  
pp. 23-37
Author(s):  
Iwona Batyk
Keyword(s):  
Foreign Trade ◽  
Russian Federation ◽  
Food Product ◽  
Food Products ◽  
Animal Origin ◽  
Trade Relations ◽  
Trade Turnover ◽  
The Russian Federation ◽  
Foreign Trade Turnover ◽  
Changes Over Time

The aim of the article is to identify the most important changes in Polish foreign trade in agri-food products with the Russian Federation in the years 2006-2015. To verify these shifts, changes over time and the structure of foreign trade in agri-food products in 2006-2015 were analyzed. The value of Poland's foreign trade turnover with Russia, the turnover balance and the share of agri-food products in trade were presented. The value of imports and exports in the categories of agri-food product groups and the dynamics of the import and export of agri-food products was also presented. The article is the result of the analysis of literature and statistical studies. Data from the Statistical Yearbook of Foreign Trade was used. Trade exchange of agri-food products is characterized by an advantage of exports from Poland to Russia. The value of trading these products was reduced after the restrictions of the Russian Federation in 2014 on the import of these products from the EU. The dynamics of exports to Russia were dependent on the category of products. The most significant changes were related to products of plant and animal origin, because their exports depended on trade barriers resulting from the geopolitical situation. To improve Polish-Russian economic and trade relations, Russia must accept EU standards for the production and veterinary and phytosanitary certification of agri-food products.

scholarly journals ICEHpsaHPS7, a novel multiple drug-resistance integrative conjugative element in Glaesserella parasuis

2020 ◽  
Author(s):  
Jiahui An ◽  
Genglin Guo ◽  
Dong Yu ◽  
Kexin Zhu ◽  
Chao Zhang ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Antimicrobial Resistance ◽  
Mobile Genetic Element ◽  
Multiple Drug Resistance ◽  
Multiple Drug ◽  
Haemophilus Parasuis ◽  
Genetic Element ◽  
Integrative Conjugative Element ◽  
Antimicrobial Resistance Genes ◽  
Integrative Conjugative Elements

Integrative conjugative elements (ICEs), a kind of novel self-transmissible mobile genetic element. In this study, a novel ICE was identified in Glaesserella (Haemophilus) parasuis. We confirmed that it could mediate the migration of antimicrobial resistance genes in G. parasuis and found that there may have been a transferring potential between different serovar strains of G. parasuis. These findings demonstrate that ICE is crucial to the horizontal transfer of antimicrobial resistance among G. parasuis.

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