Tolerance of adventive macrophyte Lemna gibba L. to copper ions: range evaluation

In recent decades, adventitization processes in flora have become more vigorous, which poses a threat to biodiversity. Adventive plant species occupy new territories, including transformed ones, and oust native species. In this regard, it is relevant to study the tolerance range of alien species to high technogenic loads since it can help assess the spread of adventive plants. The aim of the research was to study the response of the adventive macrophyte Lemna gibba L. to the copper ions action in a gradient from 0 to 10 mg/L. Duckweed fronds were incubated in model systems for six days under natural light conditions. A high positive correlation between copper content in the nutrient solution and plants was revealed. Moreover, the rise of metal accumulation was exponential. With an increase in copper concentration ≥ 0.5 mg/L, its phytotoxic effect manifested itself through the in-creased prooxidant content and the depigmentation of fronds due to the photosynthetic pigments degradation. At the maximum copper concentration (10 mg/L), the chlorophyll content was 2.5 times lower, and carotenoids, 11.5 times, as compared to the control sample. The study assessed L. gibba tolerance range to the copper ions action and identified physiological and biochemical parameters that can serve as toxicity biomarkers.

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Reference intervals of spot urine copper excretion in preschool children and potential application in pre-symptomatic screening of Wilson’s disease

10.1101/719369 ◽

2019 ◽

Author(s):

Nelson Leung-sang Tang ◽

Joannie Hui ◽

Dan Huang ◽

Man Fung Tang ◽

Xingyan Wang ◽

...

Keyword(s):

Preschool Children ◽

Test Performance ◽

Potential Application ◽

Copper Concentration ◽

Time Window ◽

Screening Program ◽

Control Sample ◽

Copper Excretion ◽

Spot Urine ◽

Presymptomatic Stage

ABSTRACTBackgroundWith spot urine collected from a large control sample of preschool children (aged 3-7 years), reference range of spot urine copper excretion indexes and their biological variation were defined.MethodsIn order to investigate their test performance in screening of Wilson disease in this age group, multiple spot urine samples from 6 WD patients diagnosed at presymptomatic stage were analysed. Cut-off values for spot urine copper concentration, copper to creatinine ratio and copper to osmolality ratio at 0.5 µmol/L, 0.1 µmol/mmol and 0.00085 µmol/mOsmol (32 µg/L, 56 µg/g creatinine and 0.054 µg/mOsmol, respectively, in conventional units) have potential application in differentiation of WD patients.ResultsThe data provides a new insight that the inter-individual variation of spot urine copper indexes (CVg) were moderate with figures around 60% which was similar to other clinically useful urine tests, such as urine albumin excretion ratio. Spot urine copper excretion strongly correlated with both urine creatinine and osmolality. And more than 95% of data points in health preschool children fell within prediction regions by linear regression suggesting a good utility of normalisation by these 2 analytes. Receiver operator curve (ROC) showed that copper to osmolality ratio was the best index with an area under curve (AUC) greater than 0.98.ConclusionsBased on the data, a new WD screening time window targeting preschool children is proposed. Application of a bivariate screening strategy using spot urine copper concentration and urine osmolality may be useful in a population screening program for preschool children.

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Kinetics of reconstitution of apotyrosinase by copper

Biochemistry and Cell Biology ◽

10.1139/o90-067 ◽

1990 ◽

Vol 68 (2) ◽

pp. 476-479

Author(s):

Donald C. Wigfield ◽

Douglas M. Goltz

Keyword(s):

Rate Constant ◽

Copper Concentration ◽

Copper Ions ◽

Copper Ion ◽

Order Rate Constant ◽

First Order ◽

Order Rate ◽

Pseudo First Order ◽

Rate Limiting ◽

Kinetics Of

The kinetics of the reconstitution reaction of apotyrosinase with copper (II) ions are reported. The reaction is pseudo first order with respect to apoenzyme and the values of these pseudo first order rate constants are reported as a function of copper (II) concentration. Two copper ions bind to apoenzyme, and if the second one is rate limiting, the kinetically relevant copper concentration is the copper originally added minus the amount used in binding the first copper ion to enzyme. This modified copper concentration is linearly related to the magnitude of the pseudo first order rate constant, up to a copper concentration of 1.25 × 10−4 M (10-fold excess), giving a second order rate constant of 7.67 × 102 ± 0.93 × 102 M−1∙s−1.Key words: apotyrosinase, copper, tyrosinase.

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Potential Phytotoxic Effect of Essential Oil of Non-Native Species Impatiens parviflora DC.

Plants ◽

10.3390/plants8070241 ◽

2019 ◽

Vol 8 (7) ◽

pp. 241 ◽

Cited By ~ 3

Author(s):

Jana Jurová ◽

Martina Matoušková ◽

Anna Wajs-Bonikowska ◽

Danuta Kalemba ◽

Marek Renčo ◽

...

Keyword(s):

Seed Germination ◽

Essential Oil ◽

Plant Species ◽

Native Species ◽

Root Elongation ◽

Invasive Plant ◽

Main Compound ◽

Impatiens Parviflora ◽

Phytotoxic Effect ◽

Dicot Species

Impatiens parviflora is non-native invasive plant species occupying large areas all over the Europe and threatens native communities by altering their species composition and reducing native biodiversity. The factor responsible for its spreading could be explained by releasing biochemical to the environment. On the other hands, high demand on secondary metabolites as potential source of new ecofriendly biocides could be beneficial. The analysis of I. parviflora essential oil (EO) led us to identify more than 60 volatiles. The main compound was hexahydrofarnesyl acetone, other dominant components were phytol, carvacrol, germacra-4(15),5,10(14)-trien-1-α-ol, and pentacosane. The potential phytotoxic effect of I. parviflora EO collected in two vegetation periods (summer and autumn) was evaluated on seed germination and root elongation of three dicot species (Raphanus sativus, Lepidum sativum, and Lactuca sativa) and on one monocot species (Triticum aestivum). The seed germination of only one dicot species, L. sativa, was affected by both EOs. In contrast, seed germination of monocot species T. aestivum was influenced only by the highest doses of EOs isolated from I. parviflora in autumn. The root elongation of tested plant species was less influenced by I. parviflora EOs. L. sativum showed sensitivity to one dose of EOs hydrodistilled in summer, while the monocot species was influenced by both EOs samples in highest doses. Our findings revealed that I. parviflora contained phenolics that were phytotoxic to the germination of some plant species, mainly at higher EOs doses, while root elongation of tested plants was not suppressed by essential oils.

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Adsorption of Copper Ions from Wastewater by Carboxymethylcellulose Sulfate

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.393-395.1098 ◽

2011 ◽

Vol 393-395 ◽

pp. 1098-1101 ◽

Cited By ~ 2

Author(s):

Yan Yu ◽

Feng Yuan Huang

Keyword(s):

Contact Time ◽

Copper Concentration ◽

Adsorption Process ◽

Ph Value ◽

Copper Ions ◽

Adsorption Capacities ◽

Monolayer Adsorption ◽

Optimum Ph ◽

Equilibrium Data ◽

Langmuir Isotherm Model

In the present paper, the ability of carboxymethylcellulose sulfate (CMC-S) for Cu(II) removal was studied. The influence of factors, such as the pH value, the initial copper concentrations, and the contact time, were investigated in detail. Atomic absorption spectrophotometer was applied to determined the concentration of Cu(II). The results showed that the adsorption process was strongly dependent on the pH value and the initial copper concentration. The optimum pH value was in the range of 6-7. The theoretical adsorption capacities for Cu(II) was 127.7 mg/g. The equilibrium data was well fitted to the Langmuir isotherm model at 25°C, which can be explained as a monolayer adsorption.

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Effect of iron and copper ions on the formation of nitrogen compounds in biacetyl/?-amino-acid model systems

European Food Research and Technology ◽

10.1007/bf01041982 ◽

1981 ◽

Vol 173 (3) ◽

pp. 204-207

Author(s):

Dharam Bal Parihar ◽

Thayur Satyanarayan Vasundhara ◽

Kottimangalam Varadachar Kumudavalli ◽

Puliyur Krishnaswamy Vijayaraghavan

Keyword(s):

Amino Acid ◽

Copper Ions ◽

Model Systems ◽

Nitrogen Compounds

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The Surface-Associated and Secreted MopE Protein of Methylococcus capsulatus (Bath) Responds to Changes in the Concentration of Copper in the Growth Medium

Applied and Environmental Microbiology ◽

10.1128/aem.69.4.2386-2388.2003 ◽

2003 ◽

Vol 69 (4) ◽

pp. 2386-2388 ◽

Cited By ~ 27

Author(s):

Odd A. Karlsen ◽

Frode S. Berven ◽

Graham P. Stafford ◽

Øivind Larsen ◽

J. Colin Murrell ◽

...

Keyword(s):

Growth Medium ◽

Copper Concentration ◽

Copper Ions ◽

Copper Stress ◽

Secreted Protein ◽

Methylococcus Capsulatus ◽

Methanotrophic Bacterium ◽

In Cells

ABSTRACT Expression of surface-associated and secreted protein MopE of the methanotrophic bacterium Methylococcus capsulatus (Bath) in response to the concentration of copper ions in the growth medium was investigated. The level of protein associated with the cells and secreted to the medium changed when the copper concentration in the medium varied and was highest in cells exposed to copper stress.

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Preliminary Investigation of Removing Copper Contamination From Water Using Algae

Volume 1: Additive Manufacturing; Advanced Materials Manufacturing; Biomanufacturing; Life Cycle Engineering; Manufacturing Equipment and Automation ◽

10.1115/msec2020-8521 ◽

2020 ◽

Author(s):

Ketan Thakare ◽

Laura Jerpseth ◽

Hongmin Qin ◽

Zhijian Pei

Keyword(s):

Heavy Metal ◽

Metal Ions ◽

Chlamydomonas Reinhardtii ◽

Exposure Time ◽

Environmental Protection Agency ◽

Copper Concentration ◽

Copper Ions ◽

Water Solution ◽

Heavy Metal Poisoning ◽

Metal Poisoning

Abstract Drinking water contaminated with metal ions can cause negative health effects in humans. Acute heavy metal poisoning can cause such symptoms as vomiting and fainting, while chronic heavy metal poisoning can lead to organ failure and death. It has previously been shown that concentration of metal ions in water solution was decreased by algae. This paper reports a study to examine the ability of two Chlamydomonas reinhardtii algae strains to remove copper ions from water solution. Chlamydomonas reinhardtii was chosen for this study because it is easy to culture, and can be used to generate strains with a higher efficiency to remove metals. In this study, the three-factor, two-level full factorial design was used to conduct experiments. Three factors were algae strain, initial copper concentration, and exposure time. Two levels of the algae strain are: cc125 — the Chlamydomonas reinhardtii strain found commonly in the wild, and AGG1 — an experimentally modified Chlamydomonas reinhardtii strain. Two levels of initial copper concentration and exposure time were 1.5 and 3 ppm, and 2.5 and 5 hours, respectively. Copper concentration in the water solution after experiments was measured using inductively coupled plasma-mass spectrometry, or ICP-MS. Statistical analysis showed that algae strain was the only factor that significantly affected percentage decrease in copper concentration, at the significance level of 0.05. The cc125 strain decreased copper concentration more efficiently than the AGG1 strain. The cc125 strain decreased copper concentration by 97% for the water solution with an initial copper concentration of 1.5 ppm, and by 90% for the solution with an initial copper concentration of 3 ppm. Copper concentrations of all solutions treated by the cc125 strain were below the Environmental Protection Agency pollution threshold level of 1.3 ppm.

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Seasonality affects phytotoxic potential of five native species of Neotropical savanna

Botany ◽

10.1139/cjb-2015-0124 ◽

2016 ◽

Vol 94 (2) ◽

pp. 81-89 ◽

Cited By ~ 4

Author(s):

Graciele Fernanda de Souza Pinto ◽

Rosana Marta Kolb

Keyword(s):

Environmental Factors ◽

Secondary Metabolites ◽

Rainy Season ◽

Native Species ◽

Aqueous Extracts ◽

Phytochemical Screening ◽

Target Species ◽

Neotropical Savanna ◽

Leaf Extracts ◽

Phytotoxic Effect

Phytotoxic secondary metabolites produced by plants have been studied as possible sources of bioherbicides. However, several environmental factors can change the secondary metabolism of plants, and thus, the production of these compounds. Among these factors, seasonality can be a source of variation in the content of all classes of secondary metabolites. In this study, we evaluated the phytotoxic effect of aqueous extracts of leaves from Byrsonima intermedia A. Juss. (Malpighiaceae), Gochnatia polymorpha (Less.) Cabrera (Asteraceae), Luehea candicans Mart. (Tiliaceae), Miconia chamissois Naudin (Melastomataceae), and Qualea cordata Spreng (Vochysiaceae) (species of the Brazilian savanna), collected both during the dry and the rainy season, on germination and seedling growth of maize and cucumber. The analysed parameters were affected by all leaf extracts collected during both seasons. However, a greater phytotoxic effect was observed when leaves were collected during the dry or during the rainy season, depending on the savanna species studied, on the target species, and on the parameters evaluated. The phytochemical screening of all extracts was also evaluated and allowed the identification of anthraquinones, triterpenoids, saponins, and tannins. The composition of extracts differed between the seasons for all species. This study highlights the importance of considering savanna seasonality when studying the phytotoxicity of the species of this biome.

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Tantalum-Nitride Diffusion Barrier Studies Using the Transient-Ion-Drift Technique for Copper Detection

MRS Proceedings ◽

10.1557/proc-612-d7.3.1 ◽

2000 ◽

Vol 612 ◽

Cited By ~ 5

Author(s):

T. Heiser ◽

C. Brochard ◽

M. Swaanen

Keyword(s):

Diffusion Barrier ◽

Copper Concentration ◽

Copper Ions ◽

Solubility Limit ◽

Depletion Region ◽

Tantalum Nitride ◽

Isochronal Annealing ◽

Ion Drift ◽

Short Annealing ◽

Annealing Experiments

AbstractThe permeability of 5 nm thick TaN, Ta and TiN diffusion barriers has been studied by monitoring the bulk copper concentration in silicon after isothermal and isochronal annealing experiments with a transient-ion-drift (TID) technique. The method estimates quantitatively the bulk copper concentration in silicon from capacitance transients of a Schottky barrier which arise when copper ions drift out of the depletion region towards the quasi-neutral region. The correlation between the copper lateral distribution and the position of the copper metal is used to distinguish between background contamination and copper originating from barrier leakage. The TID detection limit is found lower than 1012at/cm3, which makes this technique particularly well adapted for quantitative diffusion barrier studies. Isothermal and isochronal annealing experiments show that TaN fails at a higher temperature than Ta barriers. The copper concentration does not exceed the solubility limit, indicating that copper precipitates nucleate rapidly at the interface. The opposite is found in TiN covered samples where a large copper supersaturation is obtained even after short annealing times.

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