scholarly journals DETERMINATION of TOTAL FLAVONOID LEVELS on LEAF STALKS ETHANOL EXTRACT of TARO (Colocasia esculenta[L.]Schott)

2018 ◽  
Vol 1 (1) ◽  
pp. 58-66
Author(s):  
Novena Yety Lindawati
Keyword(s):  
Quantitative Analysis ◽  
Operating Time ◽  
Ethanol Extract ◽  
Total Flavonoids ◽  
Qualitative And Quantitative Analysis ◽  
Alternative Medicines ◽  
Total Flavonoids Content ◽  
Qualitative Test ◽  
Standard Solution

Taro is plant that has a pseudostalk,cylindrical and light brown bulbs. The shape of leaf is the heart in length. Leaf stalk of taro contains are saponins, flavonoids, tanins, alkaloids, steroids and terpenoids. Leaf stalk of taro can be used as an alternative medicines wound and antioxidants. Flavonoids have an important role in the biological activity of taro leaf stalk. This research aims to determine the flavonoid and total flavonoids content in the taro leaf stalk extract. The extraction is done using maceration method with 70% ethanolas solvent. The extract which is got is used for qualitative and quantitative analysis. Quercetin used as a standard solution. Quantitative analysis using UV-Vis Spectrophotometry on a wavelength of 435.5 nm and operating time at the 29th minute. The results of qualitative test showed that the extract was positive flavonoids. The average concentrations of total flavonoids was 10,2223mg QE/gram extract with %CV value of 0,3051%.

scholarly journals ANALYSIS OF TOTAL FLAVONOID LEVELS OF ETHANOL EXTRACT (Cinnamon (Cinnamomum burmanii Blume) LEAVES WITH UV-VIS SPECTROPHOTOMETRY METHOD

2021 ◽  
Vol 9 (3) ◽  
pp. 115-120
Author(s):  
Darmayuda I P P ◽  
Suardana I G ◽  
Bawa Putra A. A
Keyword(s):  
Diabetes Mellitus ◽  
Qualitative Analysis ◽  
Ethanol Extract ◽  
Total Flavonoid ◽  
Total Flavonoids ◽  
Average Content ◽  
Yellowish Green ◽  
Total Flavonoids Content ◽  
Medicinal Properties

Cinnamon plants (Cinnamomum burmani Blume), including plants that have medicinal properties for diabetes mellitus because they contain flavonoid compounds. This study aimed to determine the total flavonoids content of the ethanol extract of cinnamon leaf ethanol extract using UV-Vis spectrophotometry method. The ethanol extract of Cinnamon leaf was obtained by maceration using ethanol as a solvent.The qualitative analysis used FeCl3by forming yellowish green complex. The determination of total flavonoids content is conducted basedon AlCl3method with total flavonoidse xpressed in QE (Quercetin equivalent) at the maximum wavelength of 435 nm. The result showed that the average content of flavonoid total is 19.544 mg QE/g extract.

scholarly journals HPLC identification and determination of myricetin, quercetin, kaempferol and total flavonoids in herbal drugs

2003 ◽  
Vol 48 ◽  
pp. 25-30 ◽  
Author(s):  
Svetlana Kulevanova ◽  
Marina Stefova ◽  
Tatjana Kadifkova Panovska ◽  
Trajce Stafilov
Keyword(s):  
Quantitative Analysis ◽  
Hplc Method ◽  
Total Flavonoids ◽  
Herbal Drugs ◽  
Column Temperature ◽  
Total Flavonoids Content ◽  
Array Detection ◽  
Hydrolysis Of ◽  
Uv Range

A new and rapid HPLC method for identification and determination of myricetin, quercetin, kaempferol and total flavonoids in ten herbal drugs of Macedonian origin is presented. Preparation of samples (Uvae ursi folim, Pruni spinosae flos, Sambuci flos, Betulae folim, Primulae flos, Herniariae herba, Centaurii herba, Tiliae flos, Robiniae pseudoacaciae flos, Bursae pastoris herba) included hydrolysis of glycosides and extraction of total aglycones with ethyl acetate. HPLC analysis with UV-diode array detection was carried out on RP C18 column, using 5% acetic acid and acetonitrile in agradient elution mode and column temperature of 30 o C. The monitoring of the elution is performed in the whole UV-range and the acquisition of data for quantitative analysis at 367 nm. Screening of the extracts showed presence of quercetin in nine, kaempferol in seven and myricetin in only one sample. The quantitative analysis showed that the content of quercetin ranged from 0.026-0.506 % (m/m), while for kaempferol it was from traces to 1.246 %. Uvaeursi folium and Pruni spinosae flos were rich in content of quercetin (0.482 % and 0.506 %, respectively), while Pruni spinosae flos and Robiniae pseudoaccaciae flos contained the highest amounts of kaempferol (1.246 % and 0.892 %, respectively). Myricetin was identified and determined only in Betulae folium (0.102 %). The content of total flavonoids in the investigated samples expressed in terms of quercetin ranged from 0.040 to 1.680 %. The proposed HPLC method is convenient for use in routine analysis of myricetin, quercetin and kaempferol, as well as for estimation of total flavonoids content in herbal drugs.

scholarly journals Determination of Flavonoids Compounds of Three Species and Different Harvesting Periods in Crataegi folium Based on LC-MS/MS

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1602
Author(s):  
Ya-Ping Guo ◽  
Hong Yang ◽  
Ya-Li Wang ◽  
Xiao-Xiang Chen ◽  
Ke Zhang ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Qualitative Analysis ◽  
Quantitative Method ◽  
Total Content ◽  
Qualitative And Quantitative Analysis ◽  
Pharmacological Activities ◽  
Time Saving ◽  
Qualitative And Quantitative ◽  
First Time

Crataegi folium have been used as medicinal and food materials worldwide due to its pharmacological activities. Although the leaves of Crataegus songorica (CS), Crataegus altaica (CA) and Crataegus kansuensis (CK) have rich resources in Xinjiang, China, they can not provide insights into edible and medicinal aspects. Few reports are available on the qualitative and quantitative analysis of flavonoids compounds of their leaves. Therefore, it is necessary to develop efficient methods to determine qualitative and quantitative flavonoids compounds in leaves of CS, CA and CK. In the study, 28 unique compounds were identified in CS versus CK by qualitative analysis. The validated quantitative method was employed to determine the content of eight flavonoids of the leaves of CS, CA and CK within 6 min. The total content of eight flavonoids was 7.8–15.1 mg/g, 0.1–9.1 mg/g and 4.8–10.7 mg/g in the leaves of CS, CA and CK respectively. Besides, the best harvesting periods of the three species were from 17th to 26th September for CS, from 30th September to 15th October for CA and CK. The validated and time-saving method was successfully implemented for the analysis of the content of eight flavonoids compounds in CS, CA and CK for the first time.

Qualitative and quantitative determination of trace aldehydes and ketones in food preservative propionic acid for quality improvement

2021 ◽  
Author(s):  
Yiwen Cao ◽  
Shenjie Zhu ◽  
Lin Zhang ◽  
Qun Cui ◽  
Haiyan Wang
Keyword(s):  
Quality Improvement ◽  
Quantitative Analysis ◽  
Quantitative Determination ◽  
Propionic Acid ◽  
Qualitative And Quantitative Analysis ◽  
Food Preservative ◽  
Qualitative And Quantitative ◽  
Aldehydes And Ketones ◽  
Aldehyde Content

Aiming at the difficulty in qualitative and quantitative analysis of trace compositions in food preservative propionic acid, the trace compositions and the key components influencing the total aldehyde content in...

Determination of m-Cresol and p-Cresol in Industrial Cresols by Raman Spectrometer

2012 ◽  
Vol 468-471 ◽  
pp. 1104-1109
Author(s):  
Hui Ying Yu ◽  
Ping Yan
Keyword(s):  
Quantitative Analysis ◽  
Cross Section ◽  
Deformation Vibration ◽  
Testing Time ◽  
Qualitative And Quantitative Analysis ◽  
Raman Spectrometer ◽  
Qualitative And Quantitative ◽  
Gc Analysis

Adopting raman spectrometer to conduct qualitative and quantitative analysis of m-cresol and p-cresol, selecting the respective character peak of bencycloquidium after deformation vibration (m-cresol at 732 cm-1 and p-cresol at 841 cm-1), via experiment and calculations, the relative raman cross section of m-cresol and p-cresol can be concluded:(∂σ/∂Ω)732cm-1/(∂σ/∂Ω)1000cm-1 is 0.74 (height-fitting of the peak), and 0.61 (area-fitting of the peak). This method samples simply and rapidly, and has a short testing time, whose quantitative analysis result of m-/p-cresol is consistent with that of GC analysis.

Cross-Contaminator Exclusion Test for Dry-Ashing Determination of Protein-Bound Iodine

1955 ◽  
Vol 1 (4) ◽  
pp. 269-272 ◽  
Author(s):  
Mayer Samson ◽  
Herman Brown ◽  
Seymour Eichen
Keyword(s):  
Blood Serum ◽  
Operating Time ◽  
Dry Ashing ◽  
Qualitative Test

Abstract A simple qualitative test for iodine in blood serum has been described. It is designed to detect sera which are potential cross-contaminators in dry-ashing PBI procedures. One drop of serum is used. Added operating time in a PBI series of 20-40 determinations is not over 30 minutes.

X-Ray Fluorescence in Cotton Modification Research

1964 ◽  
Vol 8 ◽  
pp. 456-461
Author(s):  
Donald Mitcham ◽  
Biagio Piccolo ◽  
Verne W. Tripp ◽  
Robert T. O’Connor
Keyword(s):  
Quantitative Analysis ◽  
Cotton Textile ◽  
Qualitative And Quantitative Analysis ◽  
X Ray ◽  
Textile Materials ◽  
Qualitative And Quantitative ◽  
Chemically Modified ◽  
Elapsed Time

AbstractThe application of X-ray fluorescence to the qualitative and quantitative analysis of chemically modified cotton textile materials is described. The scope and flexibility of the technique have permitted the determination of more than 20 elements with, greatly reduced elapsed time compared with the corresponding spectroscopic or wet methods. Precautions to be observed in preparing standards are discussed. Results of the analysis of typical modifications and their significance in the development of cottons for specific uses are described.

scholarly journals Application of optical microscopy for the qualitative and quantitative analysis of the solid surface

2020 ◽  
Vol 20 (2) ◽  
pp. 41-64
Author(s):  
A.A. Dedkova ◽  
◽  
M.A. Makhiboroda ◽  
Keyword(s):  
Quantitative Analysis ◽  
Optical Microscopy ◽  
Surface Relief ◽  
Complex Analysis ◽  
Dark Field ◽  
Qualitative And Quantitative Analysis ◽  
Qualitative And Quantitative ◽  
Surface Measurements ◽  
Defects Analysis

The possibilities of optical microscopy for preliminary and complex analysis of structures, which are used mainly in microelectronics and micromechanics, are demonstrated. Specific examples of the use of optical microscopy for qualitative and quantitative analysis, development of technological processes, control of defects, analysis of surface relief, determination of the parameters of structures are given. Surface measurements are performed in reflected and transmitted light, using bright and dark field modes.

scholarly journals Analisis Senyawa β-Karoten pada Buah Pepaya (Carica papaya L.) Asal Kabupaten Konawe Selatan, Provinsi Sulawesi Tenggara

2021 ◽  
Vol 4 (2) ◽  
pp. 1-7
Author(s):  
Irman Idrus ◽  
Sabda Wahab ◽  
Andi Fitrah Nugraha ◽  
Syaiful Bachri
Keyword(s):  
Quantitative Analysis ◽  
The Body ◽  
Test Results ◽  
Carotene Content ◽  
Test Parameter ◽  
Qualitative Test ◽  
Uv Visible ◽  
Planting Method ◽  
Β Carotene

South Konawe District at Southeast Sulawesi Province is a producer of agriculture such as Papaya fruit ranging from 1168 quintals each year. Papaya fruit contains β-carotene which plays an important role in the formation of vitamin A in the body. This research was conducted to determine the β-carotene content of papaya fruit by using variables planting method, fruitage, and fruit condition. The mashed fruit was extracted using n-hexane: acetone: ethanol (2: 1: 1) v/v, then separated from polar and non-polar solutions. Qualitative analysis was carried out using the Carr-Price method and quantitative analysis or determination of β-carotene levels using the UV-Visible spectrophotometric method λ = 452.0 nm. The qualitative test results showed that the papaya fruit in the papaya fruit obtained by cultivation and growing wild with each variable half-ripe, ripe, and not fresh papaya identified the presence of β-carotene. The results of quantitative analysis of β-carotene levels in cultivated papaya fruit were 1.76 μL, half-ripe 1.32 μL, and cultivated young papaya 1.22 μL. In wild ripe papaya 1.75 μL, wild half-ripe papaya 1.3 μL and wild young papaya 1.21 μL. There is an effect of each test parameter starting from the parameters of planting method, fruitage, and fruit condition on β-carotene content in papaya fruit.

scholarly journals The Determination of the Iridoids from the Melampyrum species by Modern Chromatographic Methods

2011 ◽  
Vol 39 (1) ◽  
pp. 79 ◽  
Author(s):  
Melania F. MUNTEANU ◽  
Laurian VLASE
Keyword(s):  
High Performance ◽  
Iron Chloride ◽  
Qualitative And Quantitative Analysis ◽  
Light Spectrum ◽  
Qualitative And Quantitative ◽  
Chromatographic Methods ◽  
Standard Solution ◽  
Tlc Analysis ◽  
Few Data

The idea of studying the iridoids from the Melampyrum bihariense Kern has started from the few data found about the chemical composition of this species. The literature mentions the presence of iridoids found in two species of Melampyrum: Melampyrum arvense and Melampyrum cristatum. The study consists of a qualitative and quantitative analysis by modern chromatographic methods, TLC.TLC coupled with photo-densitometry as well as chromatography on the high performance column (LC/MS). The methanol extract was done from the flowers and leaves of Melamphyrum bihariense. For the TLC and HPLC, the aucubin was used as standard solution. The obtained chromatogram was examined in visible light spectrum, and then it was revealed with sulphuric anisaldehyde, emphasizing the brown coloured spots. In the TLC case, the chromatographic plate was scanned with a Shimadzu CS9000 photo densitometer after spraying with iron chloride, and for the HPLC the analytical column Atlantis HILIC was used 100 mm x 3.0 mm, 3.5 ?m (water), and the ESI source of ions (electrospray ionisation). After the TLC analysis, the iridoids concentration found in the aucubin is 0.23 mg/mL in flowers and 0.24 mg/mL in leaves, and by the LC/MS method the concentration is of 1.8125 mg/ml in flowers and 2.3037 mg/ml in leaves.

Sign in / Sign up

Export Citation Format

Share Document