Effect of Lactobacillus johnsonii CRL1647 on different parameters of honeybee colonies and bacterial populations of the bee gut

2015 ◽  
Vol 6 (5) ◽  
pp. 687-695 ◽  
Author(s):  
M.C. Audisio ◽  
D.C. Sabaté ◽  
M.R. Benítez-Ahrendts
Keyword(s):  
Lactobacillus Johnsonii ◽  
Bacterial Populations ◽  
Honey Production ◽  
Bee Colony ◽  
Enterococcus Spp ◽  
Colony Performance ◽  
Genus Lactobacillus ◽  
Bacillus Spp ◽  
Bee Colonies ◽  
Administration Schedules

Lactobacillus johnsonii CRL1647, isolated from the intestinal tract of a worker-bee in Salta, Argentina, was delivered to Apis mellifera L. honey bee colonies according to two different administration schedules: 1×105 cfu/ml every 15 days (2011) or monthly (2012). The effect of each treatment on the bee-colony performance was monitored by measuring honey production, and the prevalence of varroasis and nosemosis. Worker bees from each assay were randomly captured 3 days after administration and assayed for the following intestinal culturable and defined bacterial populations: total aerobic microorganisms, Bacillus spp. spores, Lactobacillus spp., Enterococcus spp. and enterobacteria. Interestingly, both treatments generated a similar increase in honey production in treated colonies compared to controls: 36.8% (every 15 days) and 36.3% (monthly). Nosema index always exhibited a reduction when lactobacilli were administered; in turn, Varroa incidence was lower when the lactobacilli were administered once a month. Moreover, the administration of L. johnsonii CRL1647 every 15 days produced an increase in the total number of aerobic microorganisms and in bacteria belonging to the genera Lactobacillus and Enterococcus; at the same time, a decrease was observed in the number of total spores at the end of the treatment. The number of enterobacteria was constant and remained below that of control hives at the end of the assay. On the other hand, the delivery of lactobacilli once a month only showed an increase in the number of bacteria belonging to the genus Lactobacillus; meanwhile, viable counts of the remaining microorganisms assayed were reduced. Even though it seems that both treatments were similar, those bee colonies that received L. johnsonii CRL1647 every 15 days became so strong that they swarmed.

Lactobacillus johnsonii CRL1647, isolated from Apis mellifera L. bee-gut, exhibited a beneficial effect on honeybee colonies

2011 ◽  
Vol 2 (1) ◽  
pp. 29-34 ◽  
Author(s):  
M. Audisio ◽  
M. Benítez-Ahrendts
Keyword(s):  
Lactic Acid Production ◽  
Treated Group ◽  
Early Summer ◽  
Egg Laying ◽  
Lactobacillus Johnsonii ◽  
Beneficial Effects ◽  
Colony Management ◽  
Bee Colony ◽  
Colony Performance

Lactobacillus johnsonii CRL1647, isolated from the intestinal tract of a honeybee and selected due to its high lactic acid production, was assayed as a monoculture on bee colony performance. It was delivered to the bees on a one litre of 125 g/l sugar-cane syrup with a final concentration of 105 cfu/ml lactobacilli. The bees accepted the new nourishment, which was consumed within 24-48 h and was administered in two independent trials (every 14-15 days for 3 consecutive months in one case, and once a month for 13 consecutive months in the other). From late spring – early summer (2008) the photo-records and statistical analyses revealed significant differences in the open and the operculated brood areas in the treated group compared with the control. This stimulation was observed after the first administration of the lactobacilli and maintained throughout. Also, a higher number of bees were measured in the treated group (54%) and the control (18%) with respect to the initial bees' number. Furthermore, honey storage was higher, 40% and 19%, for the treated and control groups, respectively. From December 2008 to December 2009, a similar situation was observed even though, in this trial, the lactobacilli cells were administered once a month. The in vivo results of this study are promising and indicate that a L. johnsonii CRL1647 supplement to beehives favours mainly open and operculated brood areas, demonstrating a stronger stimulation of egg-laying and will become a natural product which will assist the beekeeper both in colony management and the creation of late nuclei and/or bee packages due to its beneficial effects in the beehive colony.

scholarly journals No apparent correlation between honey bee forager gut microbiota and honey production

PeerJ ◽  
2015 ◽  
Vol 3 ◽  
pp. e1329 ◽  
Author(s):  
Melissa A. Horton ◽  
Randy Oliver ◽  
Irene L. Newton
Keyword(s):  
Honey Bee ◽  
Function Analysis ◽  
Amplicon Sequencing ◽  
Microbial Composition ◽  
Library Size ◽  
Honey Production ◽  
16S Rrna Amplicon Sequencing ◽  
Bee Colony ◽  
Bee Colonies ◽  
Colony Productivity

One of the best indicators of colony health for the European honey bee (Apis mellifera) is its performance in the production of honey. Recent research into the microbial communities naturally populating the bee gut raise the question as to whether there is a correlation between microbial community structure and colony productivity. In this work, we used 16S rRNA amplicon sequencing to explore the microbial composition associated with forager bees from honey bee colonies producing large amounts of surplus honey (productive) and compared them to colonies producing less (unproductive). As supported by previous work, the honey bee microbiome was found to be dominated by three major phyla: the Proteobacteria, Bacilli and Actinobacteria, within which we found a total of 23 different bacterial genera, including known “core” honey bee microbiome members. Using discriminant function analysis and correlation-based network analysis, we identified highly abundant members (such asFrischellaandGilliamella) as important in shaping the bacterial community; libraries from colonies with high quantities of theseOrbaceaemembers were also likely to contain fewerBifidobacteriaandLactobacillusspecies (such as Firm-4). However, co-culture assays, using isolates from these major clades, were unable to confirm any antagonistic interaction betweenGilliamellaand honey bee gut bacteria. Our results suggest that honey bee colony productivity is associated with increased bacterial diversity, although this mechanism behind this correlation has yet to be determined. Our results also suggest researchers should not base inferences of bacterial interactions solely on correlations found using sequencing. Instead, we suggest that depth of sequencing and library size can dramatically influencestatistically significantresults from sequence analysis of amplicons and should be cautiously interpreted.

scholarly journals Monitoring of Bee Colonies’ Activity During the Major Gatherings in 2017

2017 ◽  
Vol 74 (2) ◽  
pp. 92
Author(s):  
Silvia PĂTRUICĂ ◽  
Daniel Severus DEZMIREAN ◽  
Marian BURA ◽  
Robert JURCOANE ◽  
Alex SPOREA
Keyword(s):  
Low Temperatures ◽  
Heavy Rainfall ◽  
Meteorological Factors ◽  
Weather Conditions ◽  
Honey Production ◽  
Bee Colony ◽  
Acacia Honey ◽  
Honey Bee Colony ◽  
Bee Colonies

The assessment of the meteorological factors, in direct correlation with the nectar release and honey production, helps beekeepers with the organization of the technological activities within the apiary (determination of the pastoral schedule, supplementary feedings, reproduction of bee colonies, honey harvest, etc.). The study was carried out between 14th of April and 20th of July 2017, on 20 Apis mellifera carpatica bee colonies, maintained in multi-layer and Dadant beehives. The bee colonies were transported in field for rape, acacia and sunflower gathering and monitored with the help of the BeeWatch Professional device. The rape and acacia gatherings were negatively influenced by low temperatures and heavy rainfall, which impeded the capitalization of the melliferous potential. The bee colonies gathered only 5 kg rape honey and 3.9 kg acacia honey/bee colony/period. As regards the sunflower, the weather conditions were optimal for gathering, and the bee colonies gathered the supplies necessary for winter and also 5 kg honey extra. The honey production obtained by the bee colonies studied was not influenced by the beehive type; it was strongly influenced by the environmental factors, temperature and humidity.

scholarly journals Evaluation of the honey and pollen yield of Melipona interrupta bee colonies in the Amazon region

2020 ◽  
pp. 455-461
Author(s):  
Adcleia P. PIRES ◽  
Aline PACHECO ◽  
Lucieta G. MARTORANO ◽  
Jonival M. NETO ◽  
José Reinaldo da Silva Cabral de MORAES ◽  
...  
Keyword(s):  
Native Bees ◽  
Strongly Correlated ◽  
Rainy Period ◽  
Honey Production ◽  
Bee Colony ◽  
Statistical Analysis System ◽  
Analysis System ◽  
One Year ◽  
The City ◽  
Bee Colonies

The objective of this research was to evaluate the productivity of honey and pollen of Melipona interrupta (Jandaíra) colonies during a one-year period. This study was conducted in meliponiculture production colonies located in community of Coroca in the City of Santarém, Pará, from October 2016 to December 2017. All collections of meliponiculture material were conducted during the first week of each month. Characteristics such as the total number of food pots, height, diameter, and volume of honey and pollen pots, the mass of the colony, production of pollen and honey, and the productivity of the bee colony as a whole were constantly monitored. Each month, biometric measurements of fifteen colonies of native bees of the specie were conducted and analyzed in the Statistical Analysis System. The greatest honey production by these colonies was strongly correlated with the less-rainy period that occurs in the region, and this production was most pronounced during October through December.

scholarly journals Effect of Initial Strength of Honey Bee Colonies (Apis mellifera) Supered in Different Ways on Maximizing Honey Production in Nepal

2012 ◽  
Vol 56 (2) ◽  
pp. 71-81 ◽  
Author(s):  
Khem Raj Neupane ◽  
Jerzy Woyke ◽  
Jerzy Wilde
Keyword(s):  
Apis Mellifera ◽  
Honey Bee ◽  
Initial Strength ◽  
Honey Production ◽  
Colony Management ◽  
Bee Colony ◽  
Worker Brood ◽  
Highly Correlated ◽  
The Cost ◽  
Bee Colonies

Abstract A study was carried out to investigate the effect of the original strengths of honey bee colonies supered in different ways, on the production of honey by Apis mellifera bees in the Terai region of Nepal. Bee colonies of three different original strengths, in which the bees covered 5, 10 and 20 combs, were supered in three different methods. The results showed that honey production was highly correlated to the number of worker brood cells in the colonies (r = 0.96, p = 0.003). Colonies of 5 comb initial strength (CIS), as farmers’ practices in Nepal, produced the lowest amount of honey (30.1 kg per annum). Bees in colonies of 10 CIS with a deep super, produced twice as much honey (62.2 kg), and colonies of 20 CIS with deep supers produced even significantly more honey (74.5 kg). However, the relationship between the financial values of the produced honey to the cost of its production was the highest - 1.52 : 1 for colonies of 10 CIS with a deep super. Therefore, this bee colony management is recommended to the beekeepers in the Terai region and lower hills of Nepal. This fi nding has global application.

scholarly journals Mist-Netting of Migrating Bee-Eaters Positively Influences Honey Bee Colony Performance

2018 ◽  
Vol 62 (1) ◽  
pp. 67-78 ◽  
Author(s):  
Aleksandra Łangowska ◽  
Reuven Yosef ◽  
Piotr Skórka ◽  
Piotr Tryjanowski
Keyword(s):  
Honey Bee ◽  
Body Parts ◽  
Agricultural Pests ◽  
Pollination Services ◽  
Quantitative Evidence ◽  
Bee Colony ◽  
Colony Performance ◽  
Migratory Routes ◽  
The Impact ◽  
Bee Colonies

Abstract Bee-eaters (Meropidae) are considered agricultural pests and their presence provokes conflicts with beekeepers and farmers who rely on the pollination services of honey bees. This problem is often deal with through the mass killing of the birds, even though the quantitative evidence on the impact of bee-eaters on honey bee colonies is scarce. The current paper reports the performance of honey bee colonies protected with mist nets from migrating flocks of European bee-eaters Merops apiaster in Israel. In the study the weight gains of bee hives surrounded by mist nets were 6.44 times higher than that of unprotected hives (26.4 kg vs. 4.1 kg). The results confirmed that bee-eaters locally pose a problem to apiaries and potentially to the crops that require pollination. Mist- netting appeared to be an effective mitigation method for alleviating conflicts between beekeepers and bee-eaters. However, the study also showed that bees were able to differentiate between their main predator and other avian species trapped in mist nets and stung only bee-eaters. Moreover, the bees were targeting the most vulnerable body parts of birds which resulted in some bird fatalities. Therefore, due to accidental mortal- ity of birds, mist-netting is recommended only on the migratory routes in cases when bee hives cannot be moved to other areas.

scholarly journals Sublethal concentrations of clothianidin affect honey bee colony growth and hive CO2 concentration

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
William G. Meikle ◽  
John J. Adamczyk ◽  
Milagra Weiss ◽  
Janie Ross ◽  
Chris Werle ◽  
...  
Keyword(s):  
Honey Bee ◽  
Pesticide Exposure ◽  
Co2 Concentration ◽  
Colony Growth ◽  
Control Group ◽  
Brood Production ◽  
Weight Losses ◽  
Bee Colony ◽  
The Impact ◽  
Bee Colonies

AbstractThe effects of agricultural pesticide exposure upon honey bee colonies is of increasing interest to beekeepers and researchers, and the impact of neonicotinoid pesticides in particular has come under intense scrutiny. To explore potential colony-level effects of a neonicotinoid pesticide at field-relevant concentrations, honey bee colonies were fed 5- and 20-ppb concentrations of clothianidin in sugar syrup while control colonies were fed unadulterated syrup. Two experiments were conducted in successive years at the same site in southern Arizona, and one in the high rainfall environment of Mississippi. Across all three experiments, adult bee masses were about 21% lower among colonies fed 20-ppb clothianidin than the untreated control group, but no effects of treatment on brood production were observed. Average daily hive weight losses per day in the 5-ppb clothianidin colonies were about 39% lower post-treatment than in the 20-ppb clothianidin colonies, indicating lower consumption and/or better foraging, but the dry weights of newly-emerged adult bees were on average 6–7% lower in the 5-ppb group compared to the other groups, suggesting a nutritional problem in the 5-ppb group. Internal hive CO2 concentration was higher on average in colonies fed 20-ppb clothianidin, which could have resulted from greater CO2 production and/or reduced ventilating activity. Hive temperature average and daily variability were not affected by clothianidin exposure but did differ significantly among trials. Clothianidin was found to be, like imidacloprid, highly stable in honey in the hive environment over several months.

scholarly journals Oral Microbiota of the Snake Bothrops lanceolatus in Martinique

2018 ◽  
Vol 15 (10) ◽  
pp. 2122 ◽  
Author(s):  
Dabor Résière ◽  
Claude Olive ◽  
Hatem Kallel ◽  
André Cabié ◽  
Rémi Névière ◽  
...  
Keyword(s):  
Oral Cavity ◽  
Generation Cephalosporin ◽  
Oral Microbiota ◽  
Third Generation ◽  
Beta Lactams ◽  
Morganella Morganii ◽  
Enterococcus Spp ◽  
Bacillus Spp ◽  
Amoxicillin Clavulanate ◽  
Third Generation Cephalosporins

In Martinique, Bothrops lanceolatus snakebite, although relatively uncommon (~30 cases/year), may result in serious complications such as systemic thrombosis and local infections. Infections have been hypothesized to be related to bacteria present in the snake’s oral cavity. In this investigation, we isolated, identified, and studied the susceptibility to beta-lactams of bacteria sampled from the oral cavity of twenty-six B. lanceolatus specimens collected from various areas in Martinique. Microbiota from B. lanceolatus oral cavity was polymicrobial. Isolated bacteria belonged to fifteen different taxa; the most frequent being Aeromonas hydrophyla (present in 50% of the samples), Morganella morganii, Klebsiella pneumoniae, Bacillus spp., and Enterococcus spp. Analysis of antibiotic susceptibility revealed that 66.7% of the isolated bacteria were resistant to amoxicillin/clavulanate. In contrast, the majority of isolated bacteria were susceptible to the third-generation cephalosporins (i.e., 73.3% with cefotaxime and 80.0% with ceftazidime). Microbiota from B. lanceolatus oral cavity is polymicrobial with bacteria mostly susceptible to third-generation cephalosporins but rarely to amoxicillin/clavulanate. In conclusion, our findings clearly support that first-line antibiotic therapy in the B. lanceolatus-bitten patients, when there is evidence of infection, should include a third-generation cephalosporin rather than amoxicillin/clavulanate.

scholarly journals Evaluation of sanitary quality of goat milk in dairy industries from the Cariri region, state of Paraíba

2021 ◽  
Vol 10 (12) ◽  
pp. e596101220735
Author(s):  
Iara Nunes de Siqueira ◽  
Aline Antas Cordeiro Cavalcanti ◽  
Joyce Galvão de Souza ◽  
Filipe Jordão Pereira de Medeiros ◽  
João Carlos Taveira ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Listeria Monocytogenes ◽  
Pathogenic Bacteria ◽  
Goat Milk ◽  
Salmonella Spp ◽  
Pasteurized Milk ◽  
Enterococcus Spp ◽  
Bacillus Spp ◽  
Thermotolerant Coliforms ◽  
Staphylococcus Spp

The sanitary evaluation of equipment and hands is fundamental to investigate the presence of pathogens in the dairy industry. Then, this study aims to evaluate the sanitization of equipment, workers’ hands, raw and pasteurized milk in goat milk dairies in the Cariri region, state of Paraíba.  Collected 32 samples of four dairies represented by letters A, B, C, and D. The followings contents were analyzed: mesophiles, total and thermotolerant coliforms, Escherichia coli, Staphylococcus aureus, Samonella spp. and Listeria monocytogenes in the reception tank, pasteurization tank, packing machine, package, wall, workers’ hand, and each dairy’s raw and pasteurized milk. After isolation, 84 colonies were confirmed by MALDI TOF. The indicator microorganisms presented variations for the workers’ hands, while A and B stayed within the patterns. For the equipment, only dairy B was within limits. They were out of the standard for mesophiles, total coliforms, and thermotolerant regarding raw and pasteurized milk. The microorganisms, the Enterobacteriaceae family presented a higher frequency, with 77.38%, and within this family, Escherichia coli, Klebsiella spp., and Enterobacter spp. were the most prevalent. Gram-positive corresponded to 22.62%, Bacillus spp., Staphylococcus spp., Enterococcus spp., and Macrococcus caseolyticus. Listeria monocytogenes and Salmonella spp. were not isolated. These demonstrate failures in goat milk processing with pathogenic bacteria in several dairy plants, indicating the need to adjust the product’s quality control.

scholarly journals PROSPECT BUDIDAYA USAHA LEBAH MADU (Trigona sp) DENGAN METODE TOPING DAN STUP

2020 ◽  
Vol 4 (2) ◽  
pp. 13-22
Author(s):  
Safrin Edy ◽  
Laode Al Hemawan Ardi
Keyword(s):  
Honey Bee ◽  
Carrying Capacity ◽  
Honey Bees ◽  
Business Development ◽  
Feasibility Analysis ◽  
Business Opportunity ◽  
Honey Production ◽  
Business Opportunities ◽  
Additional Costs ◽  
Bee Colonies

This study aims to analyze the feasibility of the business and analyze the opportunities and threats of Trigona Ongka farm in Laompo Village, Batauga District, South Buton Regency. The research was conducted from January to February 2020 in Laompo Village, Batauga District, South Buton Regency. The sample in this study was 1 (one) honeybee breeders (Trigona sp). The method used is the independent sample t-test and the R / C feasibility analysis. Business opportunities and threats are analyzed qualitatively. The results showed that the business feasibility of Trigona sp honey bee livestock through the STUP and topping method was 1.5, (R/C > 1), meaning that each additional cost incurred resulted in a larger increase in revenue as much as IDR 1,400,000 for the STUP method and IDR 1,500,000 for the topping method instead of additional costs or simply profitable business activities. The honey bee livestock business opportunity of Trigona sp, namely honey as a favorite that is popular, relatively stable price, partially known production of the community, the location is easy to reach by vehicles, the availability of large land for business development. While the threat of Trigona sp honey bee livestock, namely: lack of environmental carrying capacity, so that environmental improvements and increasing the number of bee colonies are needed to increase honey production, do not have a product brand to be widely commercialized, product quality standardization is still poorly understood by breeders, there is no internal coordination between breeders to strengthen institutions and lack of access to assistance from capital institutions in terms of capital.  Keywords: Prospects, Business Feasibility, Honey Bees

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