In vitro evidence for efficacy in food intolerance for the multispecies probiotic formulation Ecologic® Tolerance (Syngut™).

2016 ◽  
Vol 7 (1) ◽  
pp. 111-118 ◽  
Author(s):  
I. Besseling-van der Vaart ◽  
M.D. Heath ◽  
F. Guagnini ◽  
M.F. Kramer
Keyword(s):  
Quality Criteria ◽  
Interleukin 10 ◽  
Food Intolerance ◽  
Bifidobacterium Lactis ◽  
Beneficial Effects ◽  
Specific Effects ◽  
Gut Barrier Function ◽  
Probiotic Strains ◽  
Selection Of

The beneficial effects of probiotics are currently the subject of extensive studies in health and medical research. The aim of this research was to specifically design a new probiotic formulation for supplementation in people suffering from food intolerance. The selection of strains was focussed on the capacity to influence mechanisms of action that are important in development of food intolerance with the following parameters measure: in vitro capacity to produce β-galactosidase, in vitro strengthening of the epithelial barrier, in vitro stimulation of cytokines produced by regulatory T cells, in addition to assessing fundamental quality criteria (stability, gastrointestinal (GI)-survival, multispecies concept, allergen-free). Ecologic®Tolerance/Syngut™ was subsequently developed consisting of a multispecies concept using 4 different probiotic strains (Bifidobacterium lactis W51, Lactobacillus acidophilus W22, Lactobacillus plantarum W21 and Lactococcus lactis W19). Each of these strains demonstrated ability to survive the GI-tract and strain specific effects in producing β-galactosidase, strengthening the gut barrier function after immunological-induced stress and inhibiting Th2 cytokines (IL-4, IL-5 and IL-13 (≥50%), in addition to stimulating interleukin-10 levels; thus, providing in vitro evidence for the efficacy of the selected strains to provide beneficial effects in patients suffering from food intolerance.

scholarly journals Viable and Heat-Killed Probiotic Strains Improve Oral Immunity by Elevating the IgA Concentration in the Oral Mucosa

2021 ◽  
Author(s):  
Wen-Yang Lin ◽  
Yi-Wei Kuo ◽  
Ching-Wei Chen ◽  
Yu-Fen Huang ◽  
Chen-Hung Hsu ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
The Body ◽  
Effective Dosage ◽  
Tgf Beta ◽  
Oral Tablet ◽  
Beneficial Effects ◽  
Bifidobacterium Animalis ◽  
Probiotic Strains ◽  
Oral Pathogens

AbstractOral-nasal mucosal immunity plays a crucial role in protecting the body against bacterial and viral invasion. Safe probiotic products have been used to enhance human immunity and oral health. In this study, we verified the beneficial effects of mixed viable probiotic tablets, consisting of Lactobacillus salivarius subsp. salicinius AP-32, Bifidobacterium animalis subsp. lactis CP-9, and Lactobacillus paracasei ET-66, and heat-killed probiotic tablets, consisting of L. salivarius subsp. salicinius AP-32 and L. paracasei ET-66, on oral immunity among 45 healthy participants. Participants were randomly divided into viable probiotic, heat-killed probiotic, and placebo groups. The administration of treatment lasted for 4 weeks. Saliva samples were collected at Weeks 0, 2, 4, and 6, and Lactobacillus, Bifidobacterium and Streptococcus mutans populations and IgA concentration were measured. IgA concentrations, levels of TGF-beta and IL-10 in PBMCs cells were quantified by ELISA method. Results showed that salivary IgA levels were significantly increased on administration of both the viable (119.30 ± 12.63%, ***P < 0.001) and heat-killed (116.78 ± 12.28%, ***P < 0.001) probiotics for 4 weeks. Among three probiotic strains, AP-32 would effectively increase the levels of TGF-beta and IL-10 in PBMCs. The oral pathogen Streptococcus mutans was significantly reduced on viable probiotic tablet administration (49.60 ± 31.01%, ***P < 0.001). The in vitro antibacterial test confirmed that viable probiotics effectively limited the survival rate of oral pathogens. Thus, this clinical pilot study demonstrated that oral probiotic tablets both in viable form or heat-killed form could exert beneficial effects on oral immunity via IL-10, TGB-beta mediated IgA secretion. The effective dosage of viable probiotic content in the oral tablet was 109 CFUs/g and the heat-killed oral tablet was 1 × 1010 cells/g.

scholarly journals Rhizosphere-enriched microbes as a pool to design synthetic communities for reproducible beneficial outputs

2018 ◽  
Author(s):  
Maria-Dimitra Tsolakidou ◽  
Ioannis A. Stringlis ◽  
Natalia Fanega-Sleziak ◽  
Stella Papageorgiou ◽  
Antria Tsalakou ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Fungal Pathogens ◽  
Growth Parameters ◽  
Tomato Plants ◽  
Beneficial Effects ◽  
Negative Effect ◽  
Tomato Growth ◽  
Selection Of

AbstractComposts represent a sustainable way to suppress diseases and improve plant growth. Identification of compost-derived microbial communities enriched in the rhizosphere of plants and characterization of their traits, could facilitate the design of microbial synthetic communities (SynComs) that upon soil inoculation could yield consistent beneficial effects towards plants. Here, we characterized a collection of compost-derived bacteria, previously isolated from tomato rhizosphere, forin vitroantifungal activity against soil-borne fungal pathogens and for their potential to change growth parameters inArabidopsis. We further assessed root-competitive traits in the dominant rhizospheric genusBacillus. Certain isolated rhizobacteria displayed antifungal activity against the tested pathogens and affected growth ofArabidopsis, and Bacilli members possessed several enzymatic activities. Subsequently, we designed two SynComs with different composition and tested their effect onArabidopsisand tomato growth and health. SynCom1, consisting of different bacterial genera, displayed negative effect onArabidopsis in vitro, but promoted tomato growth in pots. SynCom2, consisting of Bacilli, didn’t affectArabidopsisgrowth, enhanced tomato growth and suppressed Fusarium wilt symptoms. Overall, we found selection of compost-derived microbes with beneficial properties in the rhizosphere of tomato plants, and observed that application of SynComs on poor substrates can yield reproducible plant phenotypes.

scholarly journals Zeaxanthin: Metabolism, Properties, and Antioxidant Protection of Eyes, Heart, Liver, and Skin

Antioxidants ◽  
2019 ◽  
Vol 8 (9) ◽  
pp. 390 ◽  
Author(s):  
Ana Murillo ◽  
Siqi Hu ◽  
Maria Fernandez
Keyword(s):  
Free Radicals ◽  
Cardiovascular Health ◽  
Health Data ◽  
Provitamin A ◽  
Antioxidant Protection ◽  
Beneficial Effects ◽  
Specific Effects ◽  
Human Interventions ◽  
Antioxidant Effects

Zeaxanthin, a non-provitamin A carotenoid that belongs to the xanthophyll family, has been less studied than its isomer lutein. However, zeaxanthin has also been shown to have a number of beneficial effects for human health due to its ability to quench free radicals, exert antioxidant effects, as well as decrease inflammation. It is the purpose of this review to discuss the metabolism of zeaxanthin, including digestion, absorption, transport, and uptake by tissues, as well as the dietary or other factors which affect zeaxanthin bioavailability. In addition, this review also focuses on specific effects of this carotenoid on eye, skin, liver, and cardiovascular health. Data derived from human interventions, animal models of research, and in vitro and cell studies are discussed in this review.

scholarly journals Gut probiotic bacteria of Barbonymus gonionotus improve growth, hematological parameters and reproductive performances of the host

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Mohammad Abdus Salam ◽  
Md. Ariful Islam ◽  
Sulav Indra Paul ◽  
Md. Mahbubur Rahman ◽  
Mohammad Lutfar Rahman ◽  
...  
Keyword(s):  
Digestive Enzymes ◽  
Hematological Parameters ◽  
Gene Sequencing ◽  
Pseudomonas Stutzeri ◽  
Probiotic Bacteria ◽  
Citrobacter Freundii ◽  
Beneficial Effects ◽  
Individual Strain ◽  
Probiotic Strains

AbstractThis study aimed to isolate and identify probiotic bacteria from the gut of Barbonymus gonionotus and evaluate their effects on growth, hematological parameters, and breeding performances of the host. Five probiotic bacteria viz. Enterococcus xiangfangensis (GFB-1), Pseudomonas stutzeri (GFB-2), Bacillus subtilis (GFB-3), Citrobacter freundii (GFB-4), and P. aeruginosa (GFB-5) were isolated and identified using 16S rRNA gene sequencing. Application of a consortium of probiotic strains (1–3 × 1.35 × 109 CFU kg−1) or individual strain such as GFB-1 (1.62 × 109 CFU kg−1), GFB-2 (1.43 × 109 CFU kg−1), GFB-3 (1.06 × 109 CFU kg−1), GFB-4 (1.5 × 109 CFU kg−1) or GFB-5 (1.43 × 109 CFU kg−1feed) through feed significantly improved growth, histological and hematological parameters and reproductive performances of B. gonionotus compared to untreated control. Moreover, the application of these probiotics significantly increased gut lactic acid bacteria and activities of digestive enzymes but did not show any antibiotic resistance nor any cytotoxicity in vitro. The highest beneficial effects on treated fishes were recorded by the application of GFB-1, GFB-2, GFB-3, and a consortium of these bacteria (T2). This is the first report of the improvement of growth and health of B. gonionotus fishes by its gut bacteria.

scholarly journals Strain-Specific Probiotic Properties of Bifidobacteria and Lactobacilli for the Prevention of Diarrhea Caused by Rotavirus in a Preclinical Model

Nutrients ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 498 ◽  
Author(s):  
Ignasi Azagra-Boronat ◽  
Malén Massot-Cladera ◽  
Karen Knipping ◽  
Johan Garssen ◽  
Kaouther Ben Amor ◽  
...  
Keyword(s):  
Humoral Response ◽  
Lactobacillus Helveticus ◽  
Preclinical Model ◽  
Post Inoculation ◽  
Probiotic Properties ◽  
Beneficial Effects ◽  
Viral Shedding ◽  
Specific Effects ◽  
Probiotic Strains ◽  
Fecal Ph

Probiotic supplementation with different lactobacilli and bifidobacterial strains has demonstrated beneficial effects in infectious diarrhea caused by rotavirus (RV) in young children. Preclinical models of RV infection might be a good strategy to screen for the efficacy of new probiotic strains or to test their comparative efficacy. Neonatal Lewis rats were supplemented with Bifidobacterium breve M-16V, Lactobacillus acidophilus NCFM, Lactobacillus helveticus R0052, or Lactobacillus salivarius PS2 from days 2–14 of life. On day five, animals received RV SA-11 orally. Fecal samples were collected daily, weighed, and scored for the calculation of severity and incidence of diarrhea. In addition, fecal pH and fecal viral shedding were measured. Animals were sacrificed at the end of the study and their blood was obtained for the quantification of RV-specific immunoglobulins. RV infection was induced in ~90% of the animals. All probiotics caused a reduction of several clinical variables of severity and incidence of diarrhea, except L. salivarius PS2. L. acidophilus NCFM, B. breve M-16V, and L. helveticus R0052 seemed to be very effective probiotic strains. In addition, all Lactobacillus strains reduced the viral elimination one day post-inoculation. No differences were detected in the specific anti-RV humoral response. The present study highlights the strain-specific effects of probiotics and identifies promising probiotics for use in ameliorating and preventing RV-induced diarrhea in children, for example by including them in infant formulas.

In vitro assessment of the immunomodulatory effects of multispecies probiotic formulations for management of allergic diseases

2011 ◽  
Vol 2 (3) ◽  
pp. 183-192 ◽  
Author(s):  
N.B.M.M. Rutten ◽  
I. Besseling-Van der Vaart ◽  
M. Klein ◽  
S. De Roock ◽  
A. Vlieger ◽  
...  
Keyword(s):  
Mononuclear Cells ◽  
Cytokine Production ◽  
Allergic Diseases ◽  
Bifidobacterium Lactis ◽  
Beneficial Effects ◽  
Human Mononuclear Cells ◽  
Th2 Cytokine ◽  
Better Than

Modulation of the composition of the intestinal microbiota with probiotics could possibly offer a way of prevention or management of allergic diseases. The objective of this study was to determine the immunomodulating effects of various multispecies probiotic combinations in vitro, as preamble to application in vivo. Multispecies probiotic combinations were formulated and tested for their effects on in vitro cytokine production by human mononuclear cells and were compared to products that already have shown beneficial effects in vivo. All 4 tested combinations of probiotics showed a 40-71% decrease of Th2 cytokine production (IL-4, IL-5, and IL-13) and a variable increase of Th1 (IFN-γ) and Treg cytokine (IL-10) production compared to the medium. A specific probiotic mixture that contained Bifidobacterium breve W25, Bifidobacterium lactis ATCC SD 5219, B. lactis ATCC SD 5220, Lactobacillus plantarum W62, Lactobacillus salivarius W57 and Lactococcus lactis W19 was superior in its stimulating effect on IL-10 production (significant better than the other tested combinations; P=0.001). Modulation of in vitro cytokine production profiles can be used to differentiate between selected probiotic formulations for their immunomodulatory properties. In the future it should be demonstrated whether the immunomodulatory capacities from the multispecies probiotic formulation with the desired profile will be effective in vivo (in adolescents, followed by application in children).

scholarly journals Serendipita Species Trigger Cultivar-Specific Responses to Fusarium Wilt in Tomato

Agronomy ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 595 ◽  
Author(s):  
Negar Ghezel Sefloo ◽  
Krzysztof Wieczorek ◽  
Siegrid Steinkellner ◽  
Karin Hage-Ahmed
Keyword(s):  
Disease Incidence ◽  
Dry Mass ◽  
In Vitro Assays ◽  
Plant Interactions ◽  
Inhibitory Effects ◽  
Negative Effects ◽  
Beneficial Effects ◽  
Specific Effects ◽  
Tomato Cultivars

The endophytic fungi Serendipita indica and S. vermifera have recently gained increasing attention due to their beneficial effects on plant growth and plant health. Little is known about other species, such as S. williamsii and S. herbamans. To test their biocontrol and growth-promoting potential, susceptible and tolerant tomato cultivars (Kremser Perle and Micro-Tom, respectively) were inoculated with S. williamsii, S. herbamans, S. indica, or S. vermifera and challenged with the soilborne pathogen Fusarium oxysporum f. sp. lycopersici (Fol) in greenhouse experiments. Furthermore, in vitro assays on the direct inhibitory effects of Serendipita spp. against Fol were performed. Negative effects of Fol on phenological growth in the susceptible cultivar were alleviated by all four applied Serendipita spp. Apart from these similar effects on biometric parameters, disease incidence was only reduced by S. herbamans and S. vermifera. In the tolerant cultivar, disease parameters remained unaffected although shoot dry mass was negatively affected by S. vermifera. Direct effects of Serendipita spp. against Fol were not evident in the in vitro assays indicating an indirect effect via the host plant. Our results highlight the importance of identifying cultivar-specific effects in pathogen–endophyte–plant interactions to determine the most beneficial combinations.

Selection of probiotic strains with anti-porcine-rotavirus activities in vitro cell culture system

2014 ◽  
Vol 185 ◽  
pp. S73
Author(s):  
Yungoh Shin ◽  
Youngsung Lim ◽  
Younghoon Lim ◽  
Youri Jang ◽  
Jinsol Ham ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture System ◽  
Cell Culture System ◽  
In Vitro Cell Culture ◽  
Probiotic Strains ◽  
Porcine Rotavirus ◽  
Selection Of

scholarly journals In Vitro Multi-Species Oral Biofilms Grown in Presence of H2O2 Production-Affecting Substrates Show Health-Associated Alterations in Composition, Metabolism and Virulence.

2021 ◽  
Author(s):  
Tim Verspecht ◽  
Dorien Vermeulen ◽  
Wannes Van Holm ◽  
Naiera Zayed ◽  
Kristel Bernaerts ◽  
...  
Keyword(s):  
Therapeutic Strategy ◽  
Virulence Genes ◽  
H2o2 Production ◽  
Oral Microbiota ◽  
Periodontal Pathogens ◽  
Beneficial Effects ◽  
Commensal Species ◽  
Oral Biofilms ◽  
Selection Of

Abstract Modulation of the commensal oral microbiota is a promising preventive or therapeutic strategy for oral health and can for instance be achieved by increasing the abundance and/or activity of certain species. This study evaluated whether 10 selected substrates could modulate in vitro multi-species oral biofilms towards a more health-associated state. These substrates were chosen based on the possibility that they could stimulate H2O2 production by certain commensal species and/or increase their abundance, as previously reported or as hypothesized based on known bacterial H2O2 pathways. Biofilms grown in presence of the substrates at a clinically relevant concentration of 1%(w/v) often showed increased abundances of commensal species and decreased abundances of periodontal pathogens. Furthermore, most biofilms also showed an altered metabolic profile. Effects on the expression of a selection of virulence genes were substrate-dependent, but often a decreased expression of certain genes could be observed. In conclusion, this study found that a selection of substrates chosen for their hypothesized beneficial effects on the commensal oral microbiota were able to modulate in vitro multi-species oral biofilms towards a more health-associated state. These modulatory effects were found to be substrate-dependent.

Inability of Probiotic Bacterial Strains Lactobacillus rhamnosus HN001 and Bifidobacterium lactis HN019 To Induce Human Platelet Aggregation In Vitro

2005 ◽  
Vol 68 (11) ◽  
pp. 2459-2464 ◽  
Author(s):  
J. S. ZHOU ◽  
K. J. RUTHERFURD ◽  
H. S. GILL
Keyword(s):  
Platelet Aggregation ◽  
Infective Endocarditis ◽  
Platelet Activation ◽  
Blood Platelets ◽  
Lactobacillus Rhamnosus ◽  
Contributory Factor ◽  
Bifidobacterium Lactis ◽  
Activated Platelets ◽  
Probiotic Strains

Platelet aggregation contributes to the pathogenesis of infective endocarditis, and aggregation of platelets induced by lactobacilli is thought to be an important contributory factor in the development and progression of Lactobacillus endocarditis. The main purpose of this study was to examine the effect of immunity-enhancing probiotic strains Lactobacillus rhamnosus HN001 and Bifidobacterium lactis HN019 on the activation and aggregation of human blood platelets. Whole blood samples from healthy individuals were incubated in vitro with HN001 or HN019 and subsequently labeled with platelet-specific monoclonal antibodies, fluorescein isothiocyanate–conjugated anti-CD41a (expressed on normal platelets), and phycoerythrin-streptavidin–conjugated anti-CD62p (expressed on activated platelets) before analysis by flow cytometry. Platelet-rich plasma was used to assist the gating of the platelet cluster. ADP and epinephrine were used as the physiological platelet activation agonists. Platelet aggregation–inducing strain Streptococcus sanguis 133-79 was used as a positive control strain. The mean fluorescence intensity of phycoerythrin and the percentage of platelets expressing the CD62p marker were used to assess the degree of platelet activation. The percentage of CD62p-positive platelets and the light scatter profiles of the agonist-activated platelets were used to identify the occurrence and degree of platelet aggregation. HN001 and HN019 had no effect on spontaneous platelet activation and aggregation; they also failed to exacerbate the platelet aggregation activity induced by ADP and epinephrine. Therefore, these test probiotic strains HN001 and HN019 are less likely to participate in the pathogenesis of infective endocarditis or other thrombotic disorders with regard to platelet aggregation factors.

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