scholarly journals In vitro methane production from silages based on Cenchrus purpureus mixed with Tithonia diversifolia in different proportions

2020 ◽  
Vol 43 ◽  
pp. e51322
Author(s):  
Maria Alexandra Huertas González ◽  
Olga Lucía Mayorga Mogollón ◽  
Yuri Marcela García Saavedra ◽  
Vilma Amparo Holguín Castaño ◽  
Jairo Mora-Delgado
Keyword(s):  
Climate Change ◽  
Energy Loss ◽  
Food Production ◽  
Gompertz Model ◽  
Gas Production ◽  
Tithonia Diversifolia ◽  
Ch4 Production ◽  
Adaptation Practice ◽  
Livestock Systems

Climate change (CC) affects food production, mainly those based on livestock systems. Producers must identify adaptation strategies to ensure the production, during periods of drought, and lack of forage. Besides contributing to CC, high emissions of ruminal methane (CH4) are energy loss potentially usable for livestock production. The objective was to estimate in vitro ruminal gas production (RGP) and determine the CH4 emissions from silages. Treatments were made with forage of Cenchrus purpureus mixed with Tithonia diversifolia T1= C.purpureus at 100%; T2= C.purpureus/ T.diversifolia in 33/67 percent ratio; T3= C.purpureus/ T.diversifolia 67/33; and T4= T.diversifolia at 100%. Samples of silages were analyzed, and they were inoculated with strains of Lactobacillus paracasei (T735); then they were fermented in vacuum-sealed bags for 67 days. RGP and CH4 were measured at 2, 4, 8, 12, 18, 24, 30, 36, and 48 hours. Additionally, modeling of CH4 production kinetics was conducted, using different equations. The results indicate that the highest cumulative CH4 production was for T1. This kinetics was represented using the Gompertz model. In conclusion, the inclusion of T.diversifolia to C.purpureus silages contributes to the decrease of methane at the ruminal level, which constitutes an adaptation practice at climate change.

Effects of Tithonia diversifolia on in vitro methane production and ruminal fermentation characteristics

2016 ◽  
Vol 56 (3) ◽  
pp. 437 ◽  
Author(s):  
S. A. Terry ◽  
R. S. Ribeiro ◽  
D. S. Freitas ◽  
G. D. Delarota ◽  
L. G. R. Pereira ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Volatile Fatty Acids ◽  
Fold Increase ◽  
Gas Production ◽  
Tithonia Diversifolia ◽  
Ruminal Fermentation ◽  
Total Volatile ◽  
Treatment Groups ◽  
Ch4 Production

The present study examined the effects of Tithonia diversifolia on in vitro methane (CH4) production and ruminal fermentation characteristics. The experiment was conducted as a completely randomised design (CRD) using a control (0% T. diversifolia) and three treatment groups with different concentrations (6.9%, 15.2%, 29.2%) of T. diversifolia, which replaced up to 15.2% and 14% dry matter (DM) of fresh sugarcane and concentrates, respectively. Ruminal fluid was obtained from two ruminally cannulated non-lactating Holstein × Zebu heifers maintained on a diet consisting of T. diversifolia, fresh sugarcane and 4 kg of concentrates. The inclusion of T. diversifolia had no effect (P ≥ 0.15) on cumulative gas production (mL, mL/g incubated DM, mL/g digested DM) or in vitro DM disappearance (%). Carbon dioxide (%, mL, mL/g incubated DM) linearly decreased (P ≤ 0.001) and CH4 (%, mL, mL/g incubated DM) quadratically increased (P ≤ 0.01) with increasing concentrations of T. diversifolia replacing fresh sugarcane and concentrates. The total volatile fatty acids (mM) and acetate (A) proportion of total volatile fatty acids (mmol/100 mmol) linearly increased (P < 0.01) with the increasing inclusion of T. diversifolia. Butyrate (mmol/100 mmol) increased quadratically (P ≤ 0.02), while propionate (P; mmol/100 mmol) decreased quadratically (P < 0.02). The A : P ratio increased linearly (P < 0.0001) with increasing amounts of T. diversifolia in the diet. These results indicated that increasing the amount of Tithonia diversifolia in the substrate DM increased the A : P ratio, which resulted in a six-fold increase of CH4 production when fresh sugarcane and concentrates were replaced at up to 15.2% and 14% (DM basis), respectively.

scholarly journals Pomegranate seed oil rich in conjugated linolenic acids reduces in vitro methane production

1970 ◽  
Vol 46 (3) ◽  
pp. 325-335
Author(s):  
E. Maleki ◽  
G.Y. Meng ◽  
M. Faseleh Jahromi ◽  
R. Jorfi ◽  
A. Khoddami ◽  
...  
Keyword(s):  
Methane Production ◽  
Volatile Fatty Acids ◽  
Seed Oil ◽  
Control Diet ◽  
Nutrient Utilization ◽  
Gas Production ◽  
Metabolizable Energy ◽  
Ruminal Fermentation ◽  
Ch4 Production

The objective of this study was to determine the effect of pomegranate (Punica granatum L.) seed oil (PSO) on gas and methane (CH4) production, ruminal fermentation and microbial populations under in vitro conditions. Three treatments consisting of a control diet containing 10 mg tallow (CON); the control diet with 5 mg PSO + 5 mg tallow (MPSO) and the control diet containing 10 mg PSO (HPSO) were compared. Ten mg of the experimental fat/oil samples were inserted into a gas-tight 100 mL plastic syringe containing 30 mL of an incubation inoculum and 250 mg of a basic substrate of a hay/concentrate (1/1, w/w) mixture. In vitro gas production was recorded over 0, 2, 4, 6, 8, 10, 12 and 24 h of incubation. After 24 hours, incubation was stopped, and methane production, pH, volatile fatty acids (VFAs) and microbial counts were measured in the inoculant. Gas production at 4, 6, 8, 10, 12 and 24 h incubation, metabolizable energy and in vitro organic matter disappearance increased linearly and quadratically as level of PSO increased. Furthermore, the 10 mg PSO (HPSO) decreased CH4 production by 21.0% compared with the control (CON) group. There were no significant differences in total and individual VFA concentrations between different levels of PSO, except for butyric acid. After 24 h of incubation, methanogenesis decreased in the HPSO compared with the MPSO and CON treatments. In addition, total bacteria and protozoa counts increased with rising PSO levels, while population methanogenesis declined significantly. These results suggested that PSO could reduce methane emissions, which might be beneficial to nutrient utilization and growth in ruminants.

scholarly journals Effect of Eucalyptus globulus leaves extracts on in vitro rumen fermentation, methanogenesis, degradability and protozoa population

2018 ◽  
Vol 18 (3) ◽  
pp. 753-767 ◽  
Author(s):  
Amina Boussaada ◽  
Rabah Arhab ◽  
Serena Calabrò ◽  
Raffaella Grazioli ◽  
Maria Ferrara ◽  
...  
Keyword(s):  
Organic Matter ◽  
Ethyl Acetate ◽  
Eucalyptus Globulus ◽  
Ethyl Acetate Extract ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Aqueous Extracts ◽  
Ch4 Production ◽  
In Vitro Gas Production

Abstract The aim of the research was to evaluate the effect of three Eucalyptus globulus extracts rich in phenolic compounds, especially flavonoids, on rumen fermentation, methane (CH4) production, organic matter degradability and protozoa population using an in vitro gas production technique. Four concentrations (0, 50, 75 and 100 mg) of three Eucalyptus extracts (ethyl acetate, n-butanol and aqueous) were added to a diet of ruminants (forage: concentrate ratio 60:40) and incubated at 39°C under anaerobiosis with buffered rumen fluid. After 24 h, the fermentation fluid was analysed for ammonia-N and volatile fatty acids (VFA). Organic matter degradability (OMD) and protozoa were also determined; in vitro gas production was also recorded and CH4 concentration was measured. Compared to the control, CH4 production was significantly lower for ethyl acetate extract (P<0.05), but higher for n-butanol and aqueous extracts. Production of ammonia- N was lower in all Eucalyptus extracts (P<0.05). Propionate production (P<0.05) increased for ethyl acetate and n-butanol extracts, whereas no effect was registered for VFA, for all Eucalyptus extracts. Ethyl acetate extract decreased in vitro OMD (P<0.05), whereas n-butanol and aqueous extracts were comparable to the control. Protozoa population decreased (P<0.05) for all extracts in comparison with the control. Eucalyptus ethyl acetate extract might be promising to be used as a potent anti-methanogenic additive. Moreover, the assessment of the right dosage seems to be important to decrease methane production, without reducing feed nutritional value.

Fermentation profiles and degradability measurements in extrusa diet samples collected from brome-suppressed and undisturbed pastures and their relationship to weight gain of steers

2004 ◽  
Vol 84 (1) ◽  
pp. 105-111 ◽  
Author(s):  
M. Blümmel ◽  
E. E. Grings ◽  
M. R. Haferkamp
Keyword(s):  
Weight Gain ◽  
Gompertz Model ◽  
Gas Production ◽  
Beef Steers ◽  
N Level ◽  
Nutritive Quality ◽  
In Vitro Gas Production ◽  
Grazing Period ◽  
Gas Volume

The effects of suppression of annual bromes (Bromus japonicus Thunb. and Bromus tectorum L.) by atrazine application on the nutritive quality of extrusa diet samples (EDS) collected from the esophagus were investigated, and EDS quality estimates were compared with weight gain of grazing steers. Analysis on EDS included crude protein (CP), in vitro organic matter degradability (IVOMD), and gas production profiles in N supplemented and unsupplemented incubation media. Brome-suppression tended (P = 0.07) to increase CP content but effects on gas production kinetics and IVOMD were dependent on incubation medium N-level. In N-unsupplemented incubations, asymptotic gas production was less and rates of gas production were greater in EDS from brome-suppressed compared to undisturbed pasture. No such differences were found for N-supplemented incubations. Weight gains of steers grazing brome-suppressed pastures were 16% greater (P = 0.007) than from control pastures. The R2 for the comparison of predicted and measured gains were 0.90 (P < 0.0001), 0.96 (P < 0.0001), and 0.90 (P < 0.0001) using CP, IVOMD (N-low), and IVOMD (N-rich) as the predicting variable, respectively. Best predictions using in vitro gas production measurements were obtained from 24 h gas volume recording (R2 = 0.93, P < 0.0001). Best-fit model (sigmoidal vs. exponential) depended on grazing period and N-level, and the sigmoidal Gompertz model best described most gas production profiles. Key words: Forage quality, gas production, weight gain, beef steers

Effects of gallic acid on in vitro rumen fermentation and methane production using rumen simulation (Rusitec) and batch-culture techniques

2019 ◽  
Vol 59 (2) ◽  
pp. 277 ◽  
Author(s):  
C. Wei ◽  
J. Guyader ◽  
L. Collazos ◽  
K. A. Beauchemin ◽  
G. Y. Zhao
Keyword(s):  
Gallic Acid ◽  
Batch Culture ◽  
Methane Production ◽  
Gas Production ◽  
Short Term ◽  
Ch4 Production ◽  
N Metabolism ◽  
In Vitro Rumen Fermentation

Two experiments were conducted to investigate the effects of adding gallic acid (GA) to ruminant diets on long- and short-term in vitro rumen fermentation and methane (CH4) production, and to test possible interactions between GA and ethanol on fermentation. The first experiment was conducted using the rumen simulation technique (Rusitec), as a completely randomised block design with four replications and the following four doses of GA: 0, 5, 10 and 20 mg GA/g dry matter (DM). Ethanol was used in all treatments to increase the solubilisation of GA in rumen fluid. The experimental period lasted 16 days, of which the first 7 days were for adaptation and the subsequent 9 days were for sampling. The second experiment was a 48-h batch-culture incubation conducted as a completely randomised design with a 4 (GA dose; 0, 10, 20, and 40 mg GA/g DM) × 2 (with or without ethanol) arrangement of treatments. In the Rusitec experiment, addition of GA up to 20 mg/g DM did not affect DM disappearance (DMD), organic matter (OM) disappearance, neutral detergent-fibre disappearance (NDFD), acid detergent-fibre disappearance (ADFD) or starch disappearance (P &gt; 0.05), but crude protein disappearance was linearly decreased (P = 0.04) from 78.3% to 72.0%. Daily gas production and CH4 production expressed as mL/g DM and mL/g DMD were not affected by addition of GA (P &gt; 0.05). Addition of GA up to 20 mg/g DM increased butyrate and isovalerate production (P &lt; 0.05) and tended to increase isobutyrate (P = 0.09) and decrease heptanoate production (P = 0.07). In the batch-culture experiment, adding GA up to 40 mg/g DM linearly increased 48-h DMD, NDFD and ADFD (P &lt; 0.05) and decreased (P &lt; 0.05) CH4 expressed as mL/g DMD, mL/g NDFD and mL/g ADFD. Methane production was decreased after 24 h and 48 h only when GA was added at 10 mg/g DM without ethanol. Fermentation liquid pH and concentration of ammonia-nitrogen (ammonia-N) were also reduced (P &lt; 0.05) with an increasing concentration of GA. Treatments with ethanol notably enhanced 48-h DMD, NDFD, ADFD, gas production (mL/g DM, mL/g OM or mL/g DMD), CH4 production (mL/g DM, mL/g DMD or mL/g NDFD), total volatile fatty acid concentration, the acetate:propionate ratio, acetate, valerate, isovalerate and caproate molar proportions (P &lt; 0.01) and decreased propionate, butyrate and isobutyrate molar proportions (P &lt; 0.01). Significant dose of GA × ethanol interaction was observed only for acetate molar proportion (P = 0.03). In conclusion, our study suggests that the beneficial effects of GA on feed digestion and CH4 production may be short term, while improvements in N metabolism may be sustained over the long term. It may be useful to conduct long-term in vivo studies using a range of diets and doses to verify whether GA can be used as a feed additive to mitigate enteric CH4 production and improve N metabolism of ruminants.

scholarly journals Roughage to Concentrate Ratio and Saccharomyces cerevisiae Inclusion Could Modulate Feed Digestion and In Vitro Ruminal Fermentation

2020 ◽  
Vol 7 (4) ◽  
pp. 151
Author(s):  
Kampanat Phesatcha ◽  
Burarat Phesatcha ◽  
Metha Wanapat ◽  
Anusorn Cherdthong
Keyword(s):  
Saccharomyces Cerevisiae ◽  
High Ratio ◽  
Gas Production ◽  
Control Treatment ◽  
Ruminal Fermentation ◽  
Dry Matter Digestibility ◽  
Ch4 Production ◽  
Feed Digestibility ◽  
Live Yeast

The objective of this research was to investigate the effect of the roughage-to-concentrate (R:C) ratio and the addition of live yeast (LY) on ruminal fermentation characteristics and methane (CH4) production. The experimental design was randomly allocated according to a completely randomized design in a 4 × 4 factorial arrangement. The first factor was four rations of R:C at 80:20, 60:40, 40:60, and 20:80, and the second factor was an additional four doses of Saccharomyces cerevisiae (live yeast; LY) at 0, 2.0 × 106, 4.0 × 106, and 6.0 × 106 colony-forming unit (cfu), respectively. For the in vitro method, during the incubation, the gas production was noted at 0, 1, 2, 4, 6, 8, 10, 12, 18, 24, 48, 72, and 96 h. The rumen solution mixture was collected at 0, 4, 8, 12, and 24 h of incubating after inoculation. Cumulative gas production at 96 h was highest in the R:C ratio, at 20:80, while the addition of LY improves the kinetics and accumulation of gas (p > 0.05). Maximum in vitro dry matter digestibility (IVDMD) and in vitro organic matter digestibility (IVOMD) at 24 h after incubation were achieved at the R:C ratio 20:80 and the addition of LY at 6 × 106 cfu, which were greater than the control by 13.7% and 12.4%, respectively. Ruminal pH at 8 h after incubation decreased with an increased proportion of concentrates in the diet, whereas it was lowest when the R:C ratio was at 20:80. Increasing the proportion of a concentrate diet increased total volatile fatty acid (TVFA) and propionic acid (C3), whereas the acetic acid (C2) and C2-to-C3 ratios decreased (p < 0.05). TVFA and C3 increased with the addition of LY at 6 × 106 cfu, which was greater than the control by 11.5% and 17.2%, respectively. No interaction effect was observed between the R:C ratio and LY on the CH4 concentration. The calculated ruminal CH4 production decreased with the increasing proportion of concentrates in the diet, particularly the R:C ratio at 20:80. The CH4 production for LY addition at 6 × 106 cfu was lower than the control treatment by 17.2%. Moreover, the greatest populations of bacteria, protozoa, and fungi at 8 h after incubation were found with the addition of LY at 6 × 106 cfu, which were higher than the control by 19.0%, 20.7%, and 40.4%, respectively. In conclusion, a high ratio of roughage and the concentrate and addition of LY at 6.0 × 106 cfu of the total dietary substrate could improve rumen fermentation, improve feed digestibility, and reduce the CH4 production.

EFECTO DE DIFERENTES MATERIALES VEGETALES DE TITHONIA DIVERSIFOLIA (HEMSL.) GRAY EN LA POBLACIÓN DE METANÓGENOS Y PROTOZOOS DEL RUMEN

2019 ◽  
Vol 2 (3) ◽  
pp. 01-10
Author(s):  
Juana Luz Galindo Blanco ◽  
Oreste La O León ◽  
Tomas Ruiz Vázquez ◽  
Alfredo González Vásquez ◽  
Washington Narvaez Campana
Keyword(s):  
Experimental Design ◽  
Crude Protein ◽  
Animal Feed ◽  
Gas Production ◽  
Control Treatment ◽  
Tithonia Diversifolia ◽  
Plant Materials ◽  
In Vitro Technique ◽  
Cynodon Nlemfuensis

Tithonia diversifolia es una planta con alto potencial para la alimentación  animal. El objetivo del presente trabajo fue evaluar el efecto de diferentes materiales vegetales (mv) en la población de metanógenos y protozoos del rumen,  se empleó la técnica in vitro de producción de gases. Los tratamientos consistieron en los siguientes materiales vegetales de T. diversifolia: mv-3, mv-5, mv- 6, mv-10, mv- 13, mv-17, mv-23, mv-24 y mv-25. Éstos se compararon con un tratamiento control de pasto estrella (Cynodon nlemfuensis). Los muestreos se realizaron a las 3 horas posteriores a la incubación y se replicó 4 veces.  El diseño experimental fue completamente aleatorizado. Los valores de proteína cruda (PC)  oscilaron desde18, 26 en el mv- 3 hasta 26,40 para el caso del mv- 26. Las poblaciones de metanógenos fueron 27,7; 23,5; 21,3; 16,2; 20,0; 19,4; 12,4; 22,5 y 20,2. 1010 UFC/mL para pasto estrella y los mv de T. diversifolia 3, 5, 6, 10, 13, 17, 23, 24 y 25, respectivamente. Las poblaciones de protozoos en el tratamiento control fueron de 48. 105células/mLy 14; 11; 10; 7; 10; 9; 4; 10 y 9, para los mv -3, mv-5, mv-6, mv-10, mv-13, mv-17, mv-23, mv-24 y mv-25, respectivamente. Se destacan como los más promisorios para reducir los metanógenos y protozoos del rumen, los mv- 23 y mv -10. Se concluye que los materiales vegetales de T. diversifolia ejercen efecto depresivo en las poblaciones de metanógenos y protozoos y se  destacan los mv -23 y mv-10 como los más promisorios para estos propósitosPalabras clave: rumen, metanogénesis, titonia ABSTRACTTithonia diversifolia is a plant with high potential for animal feed. The objective of the present work was to evaluate the effect of different plant materials (vm) in the population of methanogens and rumen protozoa, the in vitro technique of gas production was used. The treatments consisted of the following plant materials of T. diversifolia: mv-3, mv-5, mv-6, mv-10, mv-13, mv-17, mv-23, mv-24 and mv-25. These were compared with a control treatment of star grass (Cynodon nlemfuensis). Samples were taken at 3 hours after incubation and replicated 4 times. The experimental design was completely randomized. Crude protein (CP) values ranged from 18.26 in mv-3 to 26.40 in the case of mv-26. The methanogen populations were 27.7; 23.5; 21.3; 16.2; 20.0; 19.4; 12.4; 22.5 and 20.2. 1010 CFU / mL for star grass and the mv of T. diversifolia 3, 5, 6, 10, 13, 17, 23, 24 and 25, respectively. The protozoa populations in the control treatment were 48. 105 cells / mL and 14; 11; 10; 7; 10; 9; 4; 10 and 9, for mv -3, mv-5, mv-6, mv-10, mv-13, mv-17, mv-23, mv-24 and mv-25, respectively. They stand out as the most promising to reduce the methanogens and protozoa of the rumen, the mv-23 and mv -10. It is concluded that the plant materials of T. diversifolia exert a depressive effect on the populations of methanogens and protozoa and the mv -23 and mv-10 stand out as the most promising for these purposes.Keywords: rumen, methanogenesis, titonia

scholarly journals Combining Crude Glycerin with Chitosan Can Manipulate In Vitro Ruminal Efficiency and Inhibit Methane Synthesis

Animals ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 37
Author(s):  
Anuthida Seankamsorn ◽  
Anusorn Cherdthong ◽  
Metha Wanapat
Keyword(s):  
Volatile Fatty Acids ◽  
Gas Production ◽  
Nutrient Digestibility ◽  
Crude Glycerin ◽  
Control Group ◽  
In Vitro Digestibility ◽  
Ruminal Fermentation ◽  
Ch4 Production ◽  
In Vitro Gas Production

It was hypothesized that the combination of glycerin and chitosan improves ruminal fermentation efficiency via an enhanced propionate (C3) and reduces in vitro CH4 production. This was explored through in vitro gas production with substrates containing crude glycerin, which replaced cassava chips in the studied ration. The experimental design was organized following a 3 × 3 factorial in completely randomized design and the arrangement of treatments were different levels of crude glycerin supplementations 0, 10.5, and 21% of total mixed ration (TMR) and chitosan levels were added at 0, 1, and 2% dry matter (DM) of substrate. Then, 0.5 g of TMR substrates were added into 40 mL bottles, together with respective doses of chitosan and then incubated at 39 °C. The dietary treatments were performed in three replicates within the incubation, and incubations were repeated on three separate days (runs). No interactions were found between crude glycerin and chitosan doses in terms of theoretical maximum of asymptotic gas production (b), rate of gas production (c), the discrete lag time prior to gas production (L), or the cumulative gas production at 96 h of incubation (p > 0.05). Cumulative gas production at 96 h of incubation was similar among the doses of crude glycerin and levels of chitosan, which ranged from 64.27 to 69.66 mL/g DM basis of substrate (p > 0.05). The concentration of ruminal NH3-N after 2 and 4 h of incubation ranged from 14.61 to 17.10 mg/dL and did not change with the addition of crude glycerin with chitosan (p > 0.05). The concentration of CH4 after 2 h of incubation did not change among treatments (p > 0.05), whereas after 4 h of incubation, CH4 synthesis was significantly reduced by enhancing doses of crude glycerin and chitosan (p < 0.05). The combination of 21% of crude glycerin in TMR with 2% chitosan depressed CH4 production as much as 53.67% when compared to the non-supplemented group. No significant crude glycerin and chitosan interaction effect was detected for in vitro digestibility of nutrients after incubation for 12 and 24 h using the in vitro gas production technique (p > 0.05). In addition, no significant changes (p > 0.05) were observed in total volatile fatty acids, acetate (C2) or butyrate content among treatments and between the main effects of crude glycerin with chitosan. At 4 h of incubation, ruminal C3 content and the C2 to C3 ratio changed significantly when crude glycerin and chitosan was added (p < 0.05). The 21% crude glycerin incorporate into TMR, in combination with 2% additional chitosan, increased C3 content by 26.41%, whereas the ratio of C2 to C3 was reduced by 31% when compared to the control group. Propionate concentration increased by 11.75% when increasing levels of chitosan at 2% of substrate, whereas the C2 to C3 ratio decreased by 13.99% compared to the 0% chitosan group. The inclusion of crude glycerin at 21% in TMR diets with chitosan supplementation at 2% enhanced ruminal propionate concentration and reduced methane production without causing any detrimental effect on the gas kinetics or nutrient digestibility.

scholarly journals 44 Effect of Quebracho (Schinopsis balansae) extract inclusion in a high roughage diet upon in vitro gas production

2020 ◽  
Vol 98 (Supplement_2) ◽  
pp. 53-54
Author(s):  
Luiz Fernando Dias Batista ◽  
Madeline E Rivera ◽  
Aaron B Norris ◽  
Jordan Adams ◽  
Roberta Cracco ◽  
...  
Keyword(s):  
Condensed Tannin ◽  
Basal Diet ◽  
Gas Production ◽  
Random Coefficients ◽  
Feed Additives ◽  
Ruminal Fermentation ◽  
Ch4 Production ◽  
In Vitro Gas Production ◽  
Schinopsis Balansae

Abstract The utilization of natural plant secondary compounds as feed additives in animal nutrition has been extensively studied because of their ability to modify digestive and metabolic functions. Condensed tannin (CT) supplementation can potentially alter ruminal fermentation, and mitigate methane (CH4) emissions. The objective of this study was to determine the effect of quebracho CT extract (QT; Schinopsis balansae) within a roughage-based diet on overall fermentability and CH4 production utilizing the in vitro gas production technique (IVGP). Twenty rumen cannulated steers (227 ± 19 kg) were randomly assigned to four dietary treatments (n=4): QT at 0, 1, 2, and 3% of DM (QT0, QT1, QT2, and QT3). A roughage-based diet containing 88% bermudagrass hay and 12% concentrate was fed daily at 2.1% of shrunk body weight. The animals were adapted to the basal diet for 24-d then introduced to predetermined treatments for 35d. Rumen inoculum was collected weekly from each steer to perform the incubations. Two hundred milligrams of air-dried base diet were incubated for 48-h with a composite rumen inoculum for each treatment over 5 wk. Kinetic analysis of cumulative 48h gas production was performed using Gasfit. Measurements of CH4 were performed via gas chromatography and digested residue was determined post-incubation. Data were analyzed using a random coefficients model. Total gas production was higher for QT0 compared to QT1 and QT3 (P = 0.001), but not different from QT2 (P = 0.554). The fractional rate of gas production was higher for QT2 compared to QT0 (P = 0.011). First and second pool gas production decreased linearly as QT inclusion increased (P = 0.042 and 0.010, respectively). There was no dietary effect in ivNDFD (P = 0.567). However, there was a linear tendency to decrease CH4 production with the addition of QT (P=0.071) likely due to changes in the microbial population.

scholarly journals In Vitro Assessment of Enteric Methane Emission Potential of Whole-Plant Barley, Oat, Triticale and Wheat

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 450
Author(s):  
Isaac A. Aboagye ◽  
Christine L. Rosser ◽  
Vern S. Baron ◽  
Karen A. Beauchemin
Keyword(s):  
Culture Technique ◽  
Gas Production ◽  
The Other ◽  
Ch4 Emission ◽  
Animal Performance ◽  
Negative Effects ◽  
Ch4 Production ◽  
Whole Plant ◽  
Enteric Methane

The study determined in vitro enteric methane (CH4) emission potential of whole-plant cereal (WPC) forages in relationship to nutrient composition, degradability, and rumen fermentation. Two varieties of each WPC (barley, oat, triticale, and wheat) were harvested from two field replications in each of two locations in central Alberta, Canada, and an in vitro batch culture technique was used to characterize gas production (GP), fermentation, and degradability. Starch concentration (g/kg dry matter (DM)) was least (p < 0.001) for oat (147), greatest for wheat (274) and barley (229), and intermediate for triticale (194). The aNDF concentration was greater for oat versus the other cereals (531 vs. 421 g/kg DM, p < 0.01). The 48 h DM and aNDF degradabilities (DMD and aNDFD) differed (p < 0.001) among the WPCs. The DMD was greatest for barley, intermediate for wheat and triticale, and least for oat (719, 677, 663, and 566 g/kg DM, respectively). Cumulative CH4 production (MP; mL) from 12 h to 48 h of incubation was less (p < 0.001) for oat than the other cereals, reflecting its lower DMD. However, CH4 yield (MY; mg of CH4/g DM degraded) of barley and oat grown at one location was less than that of wheat and triticale (28 vs. 31 mg CH4/g DM degraded). Chemical composition failed to explain variation in MY (p = 0.35), but it explained 45% of the variation in MP (p = 0.02). Variation in the CH4 emission potential of WPC was attributed to differences in DMD, aNDFD, and fermentation end-products (R2 ≥ 0.88; p < 001). The results indicate that feeding whole-plant oat forage to ruminants may decrease CH4 emissions, but animal performance may also be negatively affected due to lower degradability, whereas barley forage may ameliorate emissions without negative effects on animal performance.

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