Detection of Sodium Ions by SPR Sensor Using Modified Self-Assembled Calix[4]Arene Deravative Monolayer

2015 ◽  
Vol 799-800 ◽  
pp. 915-918
Author(s):  
M. Benounis ◽  
Nicole Jaffrezic ◽  
Isabelle Bonnamour ◽  
Nadhir Messai
Keyword(s):  
Impedance Spectroscopy ◽  
Limit Of Detection ◽  
High Sensitivity ◽  
Gold Surface ◽  
Sodium Ions ◽  
Sensor Surface ◽  
Detection Range ◽  
Spr Sensor ◽  
Linear Detection ◽  
Influence Of Ph

A new SPR sensor surface based onself-assembled-Calix [4] arene-derivative-monolayer was proposed for the detection of sodium in water. The immobisation of claixarene onto the gold surface was confirmed by impedance spectroscopy (EIS). Three alkaliions were used K+, Na+and Ca2+and the influence of pH on ions detection was studied and optimized. The Calix [4] arene-gold SPR sensor developed was characterized by low limit of detection (LOD) for about 10-10M, high sensitivity and wide linear detection range between 10-6M and 10-14M.

scholarly journals Multiformity of Photoelectron Storages in Functionalized Carbon Nitrides Enabling Reversible and Adaptable Colorimetric Sensing

2021 ◽  
Author(s):  
Dan Han ◽  
Hong Yang ◽  
Zhixin Zhou ◽  
Kaiqing Wu ◽  
Jin Ma ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Raw Materials ◽  
High Sensitivity ◽  
Terminal Group ◽  
Blue Color ◽  
Colorimetric Sensing ◽  
Electron Hole ◽  
Detection Range ◽  
Linear Detection ◽  
Easy Operation

Colorimetric sensing has been widely used for centuries across diverse fields, thanks to easy operation with no electricity and uncompromised high sensitivity. However, the limited number of chromogenic systems hampers its broader applications. Here, we reported that carbon nitride (CN), the raw materials-abundant and cheap semiconductors with photoelectron storage capability, can be developed as a new chromogenic platform for colorimetric sensing. Beyond most photoelectron storage materials that only demonstrated blue color in the excited state, CN could also exhibit brown color by terminal group functionalization. The experiments and DFT theoretical calculation revealed the origin of the unusual two types of color switches. Cyano and carbonyl terminal groups in CN elongated the centroids distance of electron/hole and stabilized the excited states through a physical and electrochemical pathway, respectively; meanwhile, the counter cations strengthened these processes. As a result, the CN-derived colorimetric O2 sensors demonstrated excellent reversibility in recycling hundreds of times for detection, and exhibited adaptable limit of detection and linear detection range, which was superior to commercial O2 sensors, especially for complex systems with broad variable concentrations.

scholarly journals Ratiometric Fluorescent Biosensors for Glucose and Lactate Using an Oxygen-Sensing Membrane

Biosensors ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 208
Author(s):  
Hong Dinh Duong ◽  
Jong Il Rhee
Keyword(s):  
Limit Of Detection ◽  
Oxygen Sensing ◽  
High Sensitivity ◽  
Glucose Sensing ◽  
Oxidation Reactions ◽  
Lactate Oxidase ◽  
Lactate Oxidation ◽  
Detection Range ◽  
Quenching Effect ◽  
Sensing Membrane

In this study, ratiometric fluorescent glucose and lactate biosensors were developed using a ratiometric fluorescent oxygen-sensing membrane immobilized with glucose oxidase (GOD) or lactate oxidase (LOX). Herein, the ratiometric fluorescent oxygen-sensing membrane was fabricated with the ratio of two emission wavelengths of platinum meso-tetra (pentafluorophenyl) porphyrin (PtP) doped in polystyrene particles and coumarin 6 (C6) captured into silica particles. The operation mechanism of the sensing membranes was based on (i) the fluorescence quenching effect of the PtP dye by oxygen molecules, and (ii) the consumption of oxygen levels in the glucose or lactate oxidation reactions under the catalysis of GOD or LOX. The ratiometric fluorescent glucose-sensing membrane showed high sensitivity to glucose in the range of 0.1–2 mM, with a limit of detection (LOD) of 0.031 mM, whereas the ratiometric fluorescent lactate-sensing membrane showed the linear detection range of 0.1–0.8 mM, with an LOD of 0.06 mM. These sensing membranes also showed good selectivity, fast reversibility, and stability over long-term use. They were applied to detect glucose and lactate in artificial human serum, and they provided reliable measurement results.

scholarly journals Label-Free Electrochemical Biosensor Based on Au@MoS₂–PANI for Escherichia coli Detection

Chemosensors ◽  
2021 ◽  
Vol 9 (3) ◽  
pp. 49
Author(s):  
Pushap Raj ◽  
Man Hwan Oh ◽  
Kyudong Han ◽  
Tae Yoon Lee
Keyword(s):  
Escherichia Coli ◽  
Bacterial Infections ◽  
Limit Of Detection ◽  
Bacterial Pathogens ◽  
Cost Effective ◽  
Covalent Immobilization ◽  
Label Free ◽  
Self Assembled Monolayer ◽  
Detection Range ◽  
Linear Detection

Bacterial infections have become a significant challenge in terms of public health, the food industry, and the environment. Therefore, it is necessary to address these challenges by developing a rapid, cost-effective, and easy-to-use biosensor for early diagnosis of bacterial pathogens. Herein, we developed a simple, label-free, and highly sensitive immunosensor based on electrochemical detection using the Au@MoS₂–PANI nanocomposite. The conductivity of the glassy carbon electrode is greatly enhanced using the Au@MoS₂–PANI nanocomposite and a self-assembled monolayer of mercaptopropionic acid on the gold nanoparticle surface was employed for the covalent immobilization of antibodies to minimize the nonspecific adsorption of bacterial pathogens on the electrode surface. The biosensor established a high selectivity and sensitivity with a low limit of detection of 10 CFU/mL, and detected Escherichia coli within 30 min. Moreover, the developed biosensor demonstrated a good linear detection range, practical utility in urine samples, and electrode regenerative studies.

scholarly journals Citric Acid Capped CdS Quantum Dots for Fluorescence Detection of Copper Ions (II) in Aqueous Solution

Nanomaterials ◽  
2018 ◽  
Vol 9 (1) ◽  
pp. 32 ◽  
Author(s):  
Zhezhe Wang ◽  
Xuechun Xiao ◽  
Tong Zou ◽  
Yue Yang ◽  
Xinxin Xing ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Quantum Dots ◽  
Citric Acid ◽  
Limit Of Detection ◽  
Copper Ions ◽  
High Sensitivity ◽  
Fluorescence Sensor ◽  
Cds Quantum Dots ◽  
Detection Range ◽  
Cds Qds

Citric acid capped CdS quantum dots (CA-CdS QDs), a new assembled fluorescent probe for copper ions (Cu2+), was synthesized successfully by a simple hydrothermal method. In this work, the fluorescence sensor for the detection of heavy and transition metal (HTM) ions has been extensively studied in aqueous solution. The results of the present study indicate that the obtained CA-CdS QDs could detect Cu2+ with high sensitivity and selectivity. It found that the existence of Cu2+ has a significant fluorescence quenching with a large red shifted (from greenish-yellow to yellowish-orange), but not in the presence of 17 other HTM ions. As a result, Cu2S, the energy level below the CdS conduction band, could be formed at the surface of the CA-CdS QDs and leads to the quenching of fluorescence of CA-CdS QDs. Under optimal conditions, the copper ions detection range using the synthesized fluorescence sensor was 1.0 × 10‒8 M to 5.0 × 10‒5 M and the limit of detection (LOD) is 9.2 × 10‒9 M. Besides, the as-synthesized CA-CdS QDs sensor exhibited good selectivity toward Cu2+ relative to other common metal ions. Thus, the CA-CdS QDs has potential applications for detecting Cu2+ in real water samples.

scholarly journals D-shaped plastic optical fibre aptasensor for fast thrombin detection in nanomolar range

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Nunzio Cennamo ◽  
Laura Pasquardini ◽  
Francesco Arcadio ◽  
Lia E. Vanzetti ◽  
Alessandra Maria Bossi ◽  
...  
Keyword(s):  
Optical Fibre ◽  
Photoelectron Spectroscopy ◽  
Limit Of Detection ◽  
Low Cost ◽  
Gold Surface ◽  
Coagulation Cascade ◽  
Self Assembled Monolayer ◽  
Detection Range ◽  
Clinical Biomarkers ◽  
Static Contact

AbstractThe development of optical biosensors for the rapid and costless determination of clinical biomarkers is of paramount importance in medicine. Here we report a fast and low-cost biosensor based on a plasmonic D-shaped plastic optical fibre (POF) sensor derivatized with an aptamer specific for the recognition of thrombin, the target marker of blood homeostasis and coagulation cascade. In particular, we designed a functional interface based on a Self Assembled Monolayer (SAM) composed of short Poly Ethylene Glycol (PEG) chains and biotin-modified PEG thiol in ratio 8:2 mol:mol, these latter serving as baits for the binding of the aptamer through streptavidin-chemistry. The SAM was studied by X-ray Photoelectron Spectroscopy (XPS) analysis, static contact angle (CA), Surface Plasmon Resonance (SPR) in POFs, and fluorescence microscopy on gold surface. The optimized SAM composition enabled the immobilization of about 112 ng/cm2 of aptamer. The thrombin detection exploiting POF-Aptasensor occurred in short times (5–10 minutes), the reached Limit of Detection (LOD) was about 1 nM, and the detection range was 1.6–60 nM, indicating the POF-Aptasensor well addresses the needs for a low-cost, simple to use and to realize, rapid, small size and portable diagnostic platform.

scholarly journals Quantification of Neuropeptide Y with Picomolar Sensitivity Enabled by Guided-Mode Resonance Biosensors

Sensors ◽  
2019 ◽  
Vol 20 (1) ◽  
pp. 126 ◽  
Author(s):  
Mohammad G. Abdallah ◽  
Joseph A. Buchanan-Vega ◽  
Kyu J. Lee ◽  
Brett R. Wenner ◽  
Jeffery W. Allen ◽  
...  
Keyword(s):  
Neuropeptide Y ◽  
Near Infrared ◽  
Single Point ◽  
High Sensitivity ◽  
Quantitative Detection ◽  
Label Free ◽  
Sensor Surface ◽  
Detection Range ◽  
Guided Mode ◽  
Guided Mode Resonance

Assessing levels of neuropeptide Y (NPY) in the human body has many medical uses. Accordingly, we report the quantitative detection of NPY biomarkers applying guided-mode resonance (GMR) biosensor methodology. The label-free sensor operates in the near-infrared spectral region exhibiting distinctive resonance signatures. The interaction of NPY with bioselective molecules on the sensor surface causes spectral shifts that directly identify the binding event without additional processing. In the experiments described here, NPY antibodies are attached to the sensor surface to impart specificity during operation. For the low concentrations of NPY of interest, we apply a sandwich NPY assay in which the sensor-linked anti-NPY molecule binds with NPY that subsequently binds with anti-NPY to close the sandwich. The sandwich assay achieves a detection limit of ~0.1 pM NPY. The photonic sensor methodology applied here enables expeditious high-throughput data acquisition with high sensitivity and specificity. The entire bioreaction is recorded as a function of time, in contrast to label-based methods with single-point detection. The convenient methodology and results reported are significant, as the NPY detection range of 0.1–10 pM demonstrated is useful in important medical circumstances.

scholarly journals Nanostructured copper selenide as an ultrasensitive and selective non-enzymatic glucose sensor

2021 ◽  
Author(s):  
Siddesh Umapathi ◽  
Harish Singh ◽  
Jahangir Masud ◽  
Manashi Nath
Keyword(s):  
Detection Limit ◽  
High Efficiency ◽  
Glucose Sensor ◽  
High Sensitivity ◽  
Copper Selenide ◽  
Glucose Detection ◽  
Applied Potential ◽  
Detection Range ◽  
Low Detection Limit ◽  
Linear Detection

CuSe nanostructures exhibit high-efficiency for glucose detection with high sensitivity (19.419 mA mM−1 cm−2) and selectivity at low applied potential (0.15 V vs. Ag|AgCl), low detection limit (0.196 μM) and linear detection range (100 nM to 40 μM).

Crystal-Reconstructed BiVO4 Semiconductor Photoelectrochemical Sensor for Ultra-Sensitive Tumor Biomarkers Detection

2022 ◽  
Author(s):  
Yang Li ◽  
Xianying Dai ◽  
Lin He ◽  
Yuyu Bu ◽  
Jin-Ping Ao
Keyword(s):  
Laser Treatment ◽  
Limit Of Detection ◽  
High Energy ◽  
Tumor Biomarkers ◽  
Treatment Technique ◽  
Detection Range ◽  
Linear Detection ◽  
Photoelectrochemical Sensor ◽  
Energy Laser ◽  
High Energy Laser

In this work, we develop a crystal-reconstructed-BiVO4 aptamer photoelectrochemical (PEC) biosensor by high-energy laser treatment technique. This biosensor achieves a limit of detection (LOD) (0.82 ag/mL), linear detection range (1...

Quick and Sensitive Detection of Prion Disease-Associated Isoform (PrPSc) Using a Novel Gold Surface/PrPSc/Gold Nanoparticles Sandwich SPR Detection Assay

2017 ◽  
Vol 48 ◽  
pp. 18-28 ◽  
Author(s):  
Zhi Chao Lou ◽  
Jin Qiang Sun ◽  
Jin Feng Wan ◽  
Xiao Hong Zhang ◽  
Hai Qian Zhang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Surface Plasmon ◽  
Prion Disease ◽  
Gold Surface ◽  
Disease Diagnosis ◽  
Detection Range ◽  
Detection Assay ◽  
Linear Detection ◽  
Presymptomatic Stage ◽  
Bare Gold

Prion protein has drawn great attention due to its pathological potential to prion diseases. Discriminate and detection of the trace quantities PrPSc is an important measure for prion disease diagnosis at the presymptomatic stage. In this study, we developed a novel sandwich surface plasmon resonance (SPR) assay for the detection of PrPSc, involving bare gold surfaces and bare gold nanoparticles. PrPSc can be captured by the SPR sensing surface via a surface assisted coupling reaction between its intra-molecular disulfide bond and the gold atoms, while PrPC cannot bind to the gold surface strongly. The gold nanoparticles were proved to amplify the SPR detection signals via the coupling of their localized surface plasmon (LSP) with the propagating plasmon on the SPR gold surface. Our results confirmed that the bare SPR gold surface successfully captured the PrPSc from the solution with a LOD of 0.5ng/mL and a linear detection range from 0.5ng/mL to 500ng/mL. Injecting the gold nanoparticles after PrPSc yielded a dramatic enhancement of signal, with a lower LOD of 0.001ng/mL and a linear detection range from 0.001ng/mL to 10ng/mL. The gold nanoparticles permitted 4 to 322-fold increase of the signals. The required detection time was controlled in 15 min. PrPC, cys-protein G and their mixtures with PrPSc, were also detected via this sandwich SPR detection assay. Atomic force microscope (AFM) was used to evaluate the surface morphology of the SPR gold substrate after the detection. All the obtained results suggested that this novel SPR sandwich detection assay in our work was efficient, sensitive and specific for the detection of trace PrPSc

scholarly journals Comparative Studies of CPEs Modified with Distinctive Metal Nanoparticle-Decorated Electroactive Polyimide for the Detection of UA

Polymers ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 252
Author(s):  
Aamna Bibi ◽  
Sheng-Chieh Hsu ◽  
Wei-Fu Ji ◽  
Yi-Chi Cho ◽  
Karen S. Santiago ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Initial Step ◽  
Differential Pulse ◽  
Metal Nanoparticle ◽  
Carbon Paste ◽  
Detection Range ◽  
Au Nps ◽  
Linear Detection ◽  
Paste Electrode

In this present work, an electrochemical sensor was developed for the sensing of uric acid (UA). The sensor was based on a carbon paste electrode (CPE) modified with electroactive polyimide (EPI) synthesized using aniline tetramer (ACAT) decorated with reduced nanoparticles (NPs) of Au, Pt, and Ag. The initial step involved the preparation and characterization of ACAT. Subsequently, the ACAT-based EPI synthesis was performed by chemical imidization of its precursors 4,4′-(4.4′-isopropylidene-diphenoxy) bis (phthalic anhydride) BPADA and ACAT. Then, EPI was doped with distinctive particles of Ag, Pt and Au, and the doped EPIs were abbreviated as EPIS, EPIP and EPIG, respectively. Their structures were characterized by XRD, XPS, and TEM, and the electrochemical properties were determined by cyclic voltammetry and chronoamperometry. Among these evaluated sensors, EPI with Au NPs turned out the best with a sensitivity of 1.53 uA uM−1 UA, a low limit of detection (LOD) of 0.78 uM, and a linear detection range (LDR) of 5–50 uM UA at a low potential value of 310 mV. Additionally, differential pulse voltammetric (DPV) analysis showed that the EPIG sensor showed the best selectivity for a tertiary mixture of UA, dopamine (DA), and ascorbic acid (AA) as compared to EPIP and EPIS.

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