Screening of Lipid High Producing Mutant from Rhodotorula Glutinis by Low Ion Implantation and Study on Lipid Extration Technique

The paper studied on parameters of ion implantation into lipid producing strain Rhodotorula glutinis and lipid extration technology. It was found that the strain had a higher positive mutation rate when the output power was 10keV and the dose of N+ implantation was 80×2.6×1013 ions/cm2. Then a high-yield mutant strain D30 was obtained and it’s lipid yield which was 3.10 g/L increased by 33.05% than that of the original. Additionally, statistically-based experimental designs were applied for the optimization of lipid extraction by acid-heating coupling ultrasonic technique. By a Plackett-Burman design, it was found that three factors, treatment time of HCl (p=0.036) , ultrasonic time (p=0.0105) and rate of extracting solution (VCHCl3:VCH3OH) (p=0.0105), had significant effect on lipid extraction. Subsequently, these significant factors were optimized using response surface methodology (RSM), and the optimized parameters of lipid extraction were as follows: 34 min for treatment time of HCl, 7.5 min for ultrasonic treating time, 1.9:1 for rate of extracting solution (VCHCl3:VCH3OH). Finally the fermentation characteristic of high-yield mutation strain D30 was studied, when fermentation time was 10 d, it’s lipid yield increased to 7.81 g/L

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BNC Biosynthesis with Increased Productivity in a Newly Designed Surface Air-Flow Bioreactor

Applied Sciences ◽

10.3390/app10113850 ◽

2020 ◽

Vol 10 (11) ◽

pp. 3850

Author(s):

Izabela Cielecka ◽

Małgorzata Ryngajłło ◽

Stanisław Bielecki

Keyword(s):

Mechanical Properties ◽

Air Flow ◽

Culture Conditions ◽

Production Costs ◽

High Yield ◽

Flow Ratio ◽

Culture Time ◽

Wide Range ◽

Burman Design ◽

Plackett Burman Design

The application of bacterial cellulose (BNC) could be widely expanded if the production costs were reduced. This study aims to determine factors simultaneously affecting the yield and tensile strength of BNC in a newly designed surface air-flow bioreactor (SAF). For this purpose, a two-stage study was done. Firstly, the most important factors for high yield were determined based on the Plackett–Burman Design. Secondly, impact of the chosen variables on both responses was assessed in a wide range of factor values. The greatest influence on the yield and mechanical strength was proved for such factors as air-flow ratio, glucose concentration, and culture time. The productivity in a SAF bioreactor with controlled air-flow ratio was enhanced by 65%. In terms of mechanical properties, the stress of BNC membranes varied from 0.8 to 6.39 MPa depending on the culture conditions. The results of the performed tests make a useful basis for future optimizations.

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Optimization of Culture Parameters for Expression of Recombinant Human Basic Fibroblast Growth Factor in Pichia pastoris Using Response Surface Methodology Combining with Plackett-Burman Design

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.647.456 ◽

2013 ◽

Vol 647 ◽

pp. 456-461 ◽

Cited By ~ 1

Author(s):

Rui Jiang ◽

Li Wei Sun ◽

Yu Zhao ◽

Kai Feng ◽

Shou Yan Chen

Keyword(s):

Response Surface Methodology ◽

Growth Factor ◽

Pichia Pastoris ◽

Fibroblast Growth Factor ◽

Fibroblast Growth ◽

Fermentation Time ◽

Initial Ph ◽

Burman Design ◽

Plackett Burman Design ◽

Significant Factors

In this study, we used plackett-burman design (PBD) and response surface methodology (RSM) methods to optimize media and culture conditions for expression of recombinant human basic fibroblast growth factor (rhbFGF) in Pichia pastoris in order to effectively enhance the production of rhbFGF. PBD was used to screen the most significant factors from nine important factors, which were fermentation time, methanol, temperature, initial pH, OD600 for inducement, concentrated volume, YNB peptone, and casein. Then RSM was further used to optimize the significant parameters. Our results had shown that three significant factors selected by PBD were temperature, fermentation time, and methanol. Base on the 3-factor-3-level central composite design (CCD) of RSM, the final optimized conditions were fermentation time 126.7h, methanol 1.3%, temperature 21.3°C, initial pH 6.0±0.1, OD600 for inducement 26, concentrated volume 100 ml, YNB 13.4%, peptone 4%, and casein 0.1%. The maximum production of rhbFGF was 302.4mg/L, which was 2 times higher than the production of rhbFGF reported in the previous studies. Those results provided the foundation for further researches on higher density cultivation condition of rhbFGF explored in the fermenter and accelerate the industrialization development of rhbFGF.

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Optimization of a novel lipid extraction process from microalgae

Scientific Reports ◽

10.1038/s41598-021-99356-z ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Xiaojie Ren ◽

Chao Wei ◽

Qi Yan ◽

Xin Shan ◽

Mengyun Wu ◽

...

Keyword(s):

Water Treatment ◽

Organic Solvents ◽

Total Lipid ◽

Extraction Method ◽

Treatment Time ◽

Lipid Extraction ◽

Dry Weight ◽

Extraction Solvent ◽

Lipid Yield ◽

Chloroform Methanol

AbstractPrevious study found that the solvent extraction efficiency of lipid in microalgae could be greatly improved by washing algae cells before the second time extraction. Based on the "organic solvents–water–organic solvents" method, this research further studied the effect of four solvent systems (acetone, chloroform/methanol, chloroform/methanol/water, dichloromethane/methanol), two types of water treatment (vortex and ultrasonic), three water treatment time gradient (0 s, 30 s, 120 s) on the lipid extraction at three different microalgae growth stages (3rd day, 5th day, 9th day). The results show that the combination of water treatment type, treatment time and solvent is very important to the efficiency of lipid extraction. The total lipid extracted was generally increased by 10–30% after water treatment. Especially under the condition of 120 s vortex water treatment with dichloromethane/methanol as extraction solvent, the total lipid extracted increased by 61.14%. In addition, microalgae cells at different culture stages had different sensitivity to water treatment. In this study, under the combination of chloroform/methanol/water as extraction solvent and vortex water treatment for 120 s, the highest lipid yield was obtained on the ninth day of cell culture, which accounts 47.88% of the cell dry weight (478 mg/g cell dry weight). The changes of cell morphology and structure after water treatment were studied by scanning electron microscope, and it was found that water treatment could seriously destroy the cell membrane damaged by solvent, thus promoting the release of lipids. This study further optimizes the "solvent–water–solvent" lipid extraction method, which neither produces impurities nor damages the lipid quality, and can reduce the amount of organic solvent applied in the classical lipid extraction method with the same lipid yield, so it has a broad application prospect.

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Optimization of Cultural Conditions for Antioxidant Exopolysaccharides fromXerocomus badiusGrown in Shrimp Byproduct

BioMed Research International ◽

10.1155/2016/2043787 ◽

2016 ◽

Vol 2016 ◽

pp. 1-10 ◽

Cited By ~ 2

Author(s):

Xiujun Gao ◽

Peisheng Yan ◽

Xin Liu ◽

Jianbing Wang ◽

Jiajia Yu

Keyword(s):

Antioxidant Activity ◽

Regression Models ◽

Antioxidant Activities ◽

High Yield ◽

Box Behnken Design ◽

Steepest Ascent ◽

Cultural Conditions ◽

Total Antioxidant ◽

Burman Design ◽

Plackett Burman Design

To optimize the production conditions for exopolysaccharides with higher antioxidant activities fromXerocomus badiuscultured in shrimp byproduct medium, Plackett-Burman design, path of steepest ascent, and response surface methodology were explored. Based on the results of Plackett-Burman design and path of steepest ascent, a Box-Behnken design was applied to optimization and the regression models. The optimal cultural condition for high yield and antioxidant activity of the exopolysaccharides was determined to be 10.347% of solid-to-liquid ratio, a 4.322% content of bran powder, and a 1.217% concentration of glacial acetic acid. Culturing with the optimal cultural conditions resulted in an exopolysaccharides yield of4.588±0.346 g/L and a total antioxidant activity of2.956±0.105 U/mg. These values are consistent with the values predicted by the corresponding regression models (RSD < 5%).

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Culture Condition Improvement for Phytase Production in Solid State Fermentation by Aspergillus ficuum Using Statistical Method

ISRN Chemical Engineering ◽

10.5402/2012/479167 ◽

2012 ◽

Vol 2012 ◽

pp. 1-5 ◽

Cited By ~ 4

Author(s):

H. Jafari-Tapeh ◽

Z. Hamidi-Esfahani ◽

M. H. Azizi

Keyword(s):

Solid State ◽

Solid State Fermentation ◽

Shake Flask ◽

Methodological Approach ◽

Aspergillus Ficuum ◽

Phytase Production ◽

Fermentation Time ◽

Fermentation Method ◽

Burman Design ◽

Plackett Burman Design

The effective factors on phytase production by Aspergillus ficuum PTCC 5288 were studied using solid-state fermentation method in 250 ml shake flask. The effective process parameters on phytase production were identified using Plackett-Burman design. Four factors were identified of different variables, including glucose, moisture, MgSO4, and fermentation time, which were the most significant. The optimum levels of these significant parameters were determined through response surface methodological approach as follows: 10.14% glucose, 62.69% moisture, 0.46% MgSO4 and 119.23 h. The maximum predicted amount of phytase was 24.33 U/gds and the produced amount of phytase under these conditions was 25.6 U/gds, which indicates the efficacy of the model for prediction of phytase production content under different medium conditions.

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Optimization of β-Carotene Production from Rhodotorula glutinis ATCC 4054 Growing on Agro-industrial Substrate Using Plackett–Burman Design

Proceedings of the National Academy of Sciences India Section B Biological Sciences ◽

10.1007/s40011-017-0908-2 ◽

2017 ◽

Vol 88 (4) ◽

pp. 1637-1646

Author(s):

Sherif M. Husseiny ◽

Ahmed A. Abdelhafez ◽

Ali Abdel-Aziz Ali ◽

Hala Mohamed Sand

Keyword(s):

Rhodotorula Glutinis ◽

Burman Design ◽

Plackett Burman Design ◽

Β Carotene

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Integrated Utilization of Dairy Whey in Probiotic β-Galactosidase Production and Enzymatic Synthesis of Galacto-Oligosaccharides

Catalysts ◽

10.3390/catal11060658 ◽

2021 ◽

Vol 11 (6) ◽

pp. 658

Author(s):

Renfei Zhao ◽

Feiyu Duan ◽

Jingyi Yang ◽

Min Xiao ◽

Lili Lu

Keyword(s):

Cost Effective ◽

Lactobacillus Bulgaricus ◽

High Yield ◽

Efficient Catalyst ◽

Steepest Ascent ◽

Burman Design ◽

Statistical Designs ◽

Integrated Utilization ◽

Plackett Burman Design ◽

Central Composite

This work established an integrated utilization of dairy whey in β-galactosidase production from Lactobacillus bulgaricus and prebiotics synthesis by the probiotic enzyme. A cost-effective whey-based medium was newly developed for culturing Lactobacillus bulgaricus to produce β-galactosidase. The medium was optimized through response surface methodology (RSM) involving a series of statistical designs, such as the Plackett–Burman design, steepest ascent experiment, and central composite design. Under the optimized medium, the β-galactosidase activity of L. bulgaricus reached 2034 U/L, which was twice that produced from the traditional MRS medium. The cells of L. bulgaricus harvested from the whey-based medium were subsequently treated with lysozyme. The resulting crude enzyme was used as an efficient catalyst, which catalyzed the synthesis of the prebiotic galacto-oligosaccharides (GOS) in a high yield of 44.7% by using whey (200 g/L) as the substrate. The sugar mixture was further purified by activated charcoal adsorption, thereby yielding a high-purity level of 77.6% GOS.

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Optimization of Conditions for Extracting Muscle Protein from Grass Carp Using Response Surface Methodology

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.1004-1005.837 ◽

2014 ◽

Vol 1004-1005 ◽

pp. 837-840

Author(s):

Fu Lan Zhang ◽

Yong Le Liu ◽

Jian Yu ◽

Qiang Li ◽

Xiang Hong Li ◽

...

Keyword(s):

Response Surface Methodology ◽

Response Surface ◽

Grass Carp ◽

Protein Extraction ◽

Muscle Protein ◽

Extraction Temperature ◽

Solid Ratio ◽

Ultrasonic Time ◽

Burman Design ◽

Plackett Burman Design

Protein is the major component of muscle tissue, in order to analyze change rule of the grass carp muscle protein during storage, the Plackett–Burman design and response surface methodology were employed to optimize its extraction conditions. The results showed that pH, SDS concentration and liquid-solid ratio were the significant factors influencing response, and the final combination of the optimized parameters was generated: pH, 8.3; liquid-solid ratio, 17:1; SDS concentration, 3.50%;beta mercaptoethanol concentration,0.3%; extraction temperature, 25 °C; extraction time, 1 h; ultrasonic time,15 min. And under these conditions, the protein extraction rate increased to 92.42%.

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