Influence of Different Salts on the Degradation of Trichloroethylene under Reductive Condition

2011 ◽  
Vol 356-360 ◽  
pp. 1689-1692
Author(s):  
Feng Na Ma ◽  
Qing Jun Meng ◽  
Guo Jun Wu ◽  
Ai Qin Shan
Keyword(s):  
Electron Donor ◽  
Anaerobic Condition ◽  
Anoxic Condition ◽  
Trivalent Iron ◽  
Anaerobic Conditions ◽  
Iron Salts ◽  
Anoxic Environment ◽  
Mixed Salts ◽  
Tce Degradation

The effect of TCE cometabolism degradation with glucose as electron donor on reduction progress was investigated with enriched nitrate, sulfate, trivalent iron and their mixtures under anoxic or anaerobic conditions. The removal effect of TCE was found to be better in anoxic environment; TCE degradation was inhibited by the addition of trivalent iron salts and the mixed salts under anoxic condition or strongly inhibited by nitrate under anaerobic condition; promoted by the addition of trivalent iron salts under anaerobic condition; little inhibited by the addition of nitrate and sulfate under anoxic condition or sulfate and the mixed salts under anaerobic condition.

Oxygen concentration affects frequency and range of transconjugants for the incompatibility (Inc) P-1 and P-7 plasmids pBP136 and pCAR1

2020 ◽  
Vol 85 (4) ◽  
pp. 1005-1015
Author(s):  
Kentaro Ochi ◽  
Maho Tokuda ◽  
Kosuke Yanagiya ◽  
Chiho Suzuki-Minakuchi ◽  
Hideaki Nojiri ◽  
...  
Keyword(s):  
Oxygen Concentration ◽  
Anaerobic Condition ◽  
Environmental Samples ◽  
Aerobic Condition ◽  
Recipient Strain ◽  
Donor Strain ◽  
Anaerobic Conditions ◽  
Filter Mating ◽  
Aerobic And Anaerobic ◽  
Aerobic And Anaerobic Conditions

ABSTRACT The frequency of transconjugants were compared for the incompatibility (Inc) P-1 and P-7 plasmids pBP136 and pCAR1 under aerobic and anaerobic conditions. Filter mating assays were performed with one donor strain and one recipient strain using different donors of Pseudomonas and recipient strains, including Pseudomonas, Pantoea, and Buttiauxella. Under anaerobic condition, frequencies of transconjugants for both plasmids were 101-103-fold lower than those under aerobic condition regardless of whether aerobically or anaerobically grown donors and recipients were used. To compare the transconjugant ranges under aerobic and anaerobic conditions, conjugation was performed between the donor of pBP136 and recipient bacteria extracted from environmental samples. Several transconjugants were uniquely obtained from each aerobic or anaerobic condition. Our findings indicate that a plasmid can differently spread among bacteria depending on the oxygen concentrations of the environment.

scholarly journals Auxiliary Substrates for Elimination of Trichloroethene, Monochlorobenzene, and Benzene in a Sequential Anaerobic–Aerobic GAC Biobarrier

2007 ◽  
Vol 7 (1 & 2) ◽  
pp. 68
Author(s):  
M. Gozan ◽  
A. Mueller ◽  
A. Tiehm
Keyword(s):  
Vinyl Chloride ◽  
Reductive Dechlorination ◽  
Biological Degradation ◽  
Anaerobic Conditions ◽  
Batch Studies ◽  
Second Stage ◽  
Competitive Reactions ◽  
Novel Concept ◽  
Tce Degradation ◽  
Granulated Activated Carbon

Sequential anaerobic-aerobic barrier is a novel concept for groundwater bioremediation. Trichloroethene (TCE), monochlorobenzene (MCB), and benzene (BZ) were used as model contaminants representing contaminant cocktails frequently found in the contaminated subsurface. The autochthonous microflora from a contaminated field was inoculated to eliminate model contaminants in a set of sequential anaerobic–aerobic granulated activated carbon (GAC) columns and batch studies. In the anaerobic column, the TCE was reductively dechlorinated through cis-dichloroethene (cis-DCE), vinyl chloride (VC), and ethene (ETH). Ethanol and sucrose as auxiliary substrates were added to donate electrons. In the second stage, MCB, BZ, and the lower chlorinated metabolites of TCE degradation, i.e. cis-Dichloroethene (cisDCE) and vinyl chloride (VC), were oxidatively degraded with addition of hydrogen peroxide and nitrate. This paper examines the influence of auxiliary substrates on the biological degradation of model pollutants. In the anaerobic barrier, the auxiliary substrates supply should be maintained low but stoichiometrically adequate for supporting reductive dechlorination. Supplying higher amount of auxiliary substrates provoked competitive reactions in anaerobic conditions, such as sulfate reduction and methanogenesis. If the auxiliary substrates are not utilized completely in the anaerobic phase, the remaining compounds flow into the aerobic phase. This led to unwanted conditions, i.e. oxidation of auxiliary substrates instead of pollutant elimination, and a higher consumption of electron acceptors. In the aerobic barrier, in particular, ethene proved to be a suitable auxiliary substrate for cometabolic degradation of cisDCE.

The Research of Reduction Characteristics of Fe(III) Dissimilated by Different Activated Sludge

2013 ◽  
Vol 663 ◽  
pp. 1011-1016
Author(s):  
Jie Li ◽  
Si Yuan Zhai ◽  
Ya E Wang ◽  
Juan Juan Feng ◽  
Li Zhao
Keyword(s):  
Carbon Source ◽  
Activated Sludge ◽  
Nutrient Solution ◽  
Anaerobic Condition ◽  
Reduction Rate ◽  
Anoxic Condition ◽  
Maximum Accumulation ◽  
Conventional Activated Sludge ◽  
Reduction Characteristics ◽  
Better Than

The effect of reduction characteristics of microbe dissimilating of Fe(III) under different activated sludge, carbon source and anoxic/anaerobic condition was studied by activated sludge as inoculum under laboratory condition. The results showed that the maximum accumulation of Fe(Ⅱ) and the reduction rate of Fe(III) inoculated by biological iron mud were higher than that inoculated by conventional activated sludge. The reduction characteristics of Fe(III) was better in nutrient solution as carbon source than that in sewage as carbon source when inoculated biological iron mud. But the reduction characteristics of Fe(III) was better in sewage as carbon source than that in nutrient solution as carbon source when inoculated conventional activated sludge. From the whole test, the reduction characteristics of dissimilating Fe(III) microbe under anaerobic condition were better than that under anoxic condition.

Phosphorus uptake by Oscillatoria sp. microalgae for bioavailability under anaerobic condition to predict eutrophication

2021 ◽  
Vol 25 (11) ◽  
pp. 153-156
Author(s):  
Askal Maimulyanti
Keyword(s):  
Linear Correlation ◽  
Incubation Time ◽  
Anaerobic Condition ◽  
Water Solution ◽  
Phosphorus Uptake ◽  
Phosphate Concentration ◽  
Maximum Efficiency ◽  
Anaerobic Conditions ◽  
Algae Growth ◽  
Limiting Nutrient

Phosphorus is generally considered the limiting nutrient for algae growth in the aquatic environment. The phosphorus uptake for algae can give information for bioavailability-P. The contribution of phosphorous to bioavailability was evaluated using uptake-P in water solution under anaerobic conditions. The more is the incubation time, the more is the phosphate bioavailability. The maximum bioavailability occurred at 14 days with a phosphate concentration of 50 ppm resulting in P-available of 18.41 ppm. There was a linear correlation between P-available with P-medium with R2 = 0.97. The phosphorus bioavailability can be resulted from the equation: [P-Available] = 0.3789 x [P-medium]. The phosphate absorbed by the algae Oscillatoria sp in anaerobic conditions with initial concentrations of 10, 20, 30, 40 and 50 ppm was 5.46, 8.65, 11.63, 17.99 and 18.41 ppm, respectively. The maximum efficiency adsorption occurred in the concentration of 30 ppm with 49.18% adsorption.

scholarly journals Incubation Studies on Exchangable Zn for Varying Levels of added Zn Under Aerobic and Anaerobic Conditions in Grey Terrace Soils, Non Calcarious Floodplain Soils and Calcarious Floodplain Soils

2013 ◽  
Vol 9 (1-2) ◽  
pp. 9-15 ◽  
Author(s):  
KH Talukder ◽  
IU Ahmed ◽  
MS Islam ◽  
MS Islam ◽  
M Asaduzzaman ◽  
...  
Keyword(s):  
Anaerobic Condition ◽  
Early Stage ◽  
Aerobic Condition ◽  
Anaerobic Conditions ◽  
Floodplain Soil ◽  
Zinc Fractions ◽  
Floodplain Soils ◽  
Soil Zn ◽  
Aerobic And Anaerobic ◽  
Aerobic And Anaerobic Conditions

Fractions studies were done to know how the zinc applied to different soils was distributed in to various fractions when the soils incubated under aerobic and anaerobic condition. The added zinc provided significant increase in exchangeable Zn both under aerobic and anaerobic conditions although anaerobic condition gave lower results than aerobic condition. The higher results were obtained at early stage of incubation and it gradually reduced as the incubation period proceeded to 90 days. These results showed all most similar trends for all the soils under study. In general, added zinc showed significantly higher results to the different fractions of soil Zn both under anaerobic and aerobic incubation with very few exceptions. The highest amount of added Zn (12 kg/ha) always produced greater results than the lower doses. Only exchangeable Zn was found higher in the 1st measurement at 15 DAI then gradually decreased but in other cases, gradual increase in zinc fractions was seen as the incubation study proceed to longer duration provided with very few exceptions. In many cases, the exchangeable-Zn found higher only at 15 DAI but sharply reduced at 30 DAI. In general, the Gray Terrace Soil produced the highest results followed by Non Calcareous Gray Floodplain and the lowest results were observed in Dark Grey Floodplain & Brown Floodplain Soil. DOI: http://dx.doi.org/10.3329/jsf.v9i1-2.14643 J. Sci. Foundation, 9(1&2): 9-15, June-December 2011

MICROAEROPHILIC AND ANAEROBIC DECOLORIZATION OF AZO DYE IN RICH AND MINIMAL MEDIA BY CITROBACTER SP. STRAIN L17

2017 ◽  
Vol 79 (4) ◽  
Author(s):  
Ali Hossennia ◽  
Giek Far Chan ◽  
Noor Aini Abdul Rashid ◽  
Mohd Firdaus Abdul-Wahab
Keyword(s):  
Anaerobic Condition ◽  
Azo Dye ◽  
Biogas Production ◽  
Rich Medium ◽  
Anaerobic Conditions ◽  
Microaerophilic Condition ◽  
Degrading Bacteria ◽  
Different Temperatures ◽  
Minimal Media ◽  
Anaerobic Decolorization

This study aimed to investigate the ability of Citrobacter sp. strain L17 to decolourise azo dye in a rich medium (MP5) and three different minimal media (MMP5, MMGF11 and MM63) under microaerophilic and anaerobic conditions. Amaranth was used as the model dye in this investigation. Under microaerophilic condition, reactions were carried out at two different temperatures; 37°C and 45°C, whereas experiments with anaerobic condition were conducted only at 37°C. Results showed that, under microaerophilic condition, full decolourisation of Amaranth was achieved in all media tested at 37°C. However, at 45°C, complete decolourisation was observed only in MP5, MM63 and MMGF11 but no obvious decolourisation occurred in MMP5. On the other hand, complete decolourisation was observed in all media tested under anaerobic condition at 37°C, with the fastest decolourisation in MP5 (rich medium containing glucose and nutrient broth) and the slowest in MMP5. It was found that an inorganic buffer containing glucose at lower concentration was sufficient to achieve complete decolourisation under anaerobic condition. This finding is essential to identify a suitable medium for future study on biogas production by dye-degrading bacteria, which mostly requires anaerobic conditions.

scholarly journals Abundance and characterization of microorganisms isolated from oil palm empty fruit bunches waste under aerobic, anaerobic, and facultative anaerobic conditions

2020 ◽  
Vol 21 (9) ◽  
Author(s):  
DERMIYATI DERMIYATI ◽  
RADIX SUHARJO ◽  
MARELI TELAUMBANUA ◽  
RULLY YOSITA ◽  
ANGGI WINANDA SARI ◽  
...  
Keyword(s):  
Oil Palm ◽  
Anaerobic Condition ◽  
Bacterial Abundance ◽  
Plate Count ◽  
Cellulose Content ◽  
Anaerobic Conditions ◽  
Empty Fruit Bunches ◽  
Facultative Anaerobic ◽  
Different Characteristics

Abstract. Dermiyati, Suharjo R, Telaumbanua M, Yosita R, Sari AW, Andayani AP. 2020. Abundance and characterization of microorganisms isolated from oil palm empty fruit bunches waste under aerobic, anaerobic, and facultative anaerobic conditions. Biodiversitas 21: 4213-4220. Increased production of palm oil causes an increase in oil palm empty fruit bunches waste, while the utilization of the waste has not been carried out optimally. Oil palm empty fruit bunches contain high cellulose content which promotes the development of various types of bacteria with different characteristics. This study was aimed to determine the abundance and the characterization of bacteria that were isolated from extract suspension of oil palm empty fruit bunches in the form of a local microorganism solution under aerobic, anaerobic, and facultative anaerobic conditions. For isolation, the oil palm empty fruit extract suspension was inoculated on media Plate Count Agar Peptone and bacterial abundance was determined. Various bacterial characterization test included bacterial morphology test in the form of color and shape of macroscopic isolates, gram test using KOH 3%, fermentative oxidative test, soft rot test on potato tubers, hypersensitivity test on tobacco plant leaves, and hypo virulent test which was indicated by the presence of symptoms of disease in cucumber sprouts and indicated in the disease severity index (DSI) was carried out. The results of local microorganism solution isolation from oil palm empty fruit bunches obtained as many as 220 bacterial isolates consisting of 84 isolates in aerobic condition, 68 isolates in anaerobic condition, and 68 isolates in facultative anaerobic condition. The highest bacterial abundance in aerobic conditions was equal to 285.60 x 1010 CFU mL-1. Each bacterial isolate had different characteristics. Most of the characteristics of bacteria were turbid white (34.55%), round (69.55%), gram-positive (76.36%), fermentative (89.55%), softrot negative (71.82%), hypersensitive negative (97.27%), and virulent (74.55%).

scholarly journals EFFECTS OF PH SOAKING SOLUTIONS AND HYPOXIA/ANAEROBIC TREAMENT ON GABA ACCUMULATION IN GERMINATED MUNG BEAN (VIGNA RADIATA L.)

2017 ◽  
Vol 55 (2) ◽  
pp. 150 ◽  
Author(s):  
Trung Nhat Truong ◽  
Nguyen Tuong Danh ◽  
Dong Thi Anh Dao
Keyword(s):  
Anaerobic Condition ◽  
Vigna Radiata ◽  
Mung Bean ◽  
Ph Value ◽  
Aerobic Condition ◽  
Culture Conditions ◽  
Anaerobic Conditions ◽  
Gaba Production ◽  
Gaba Content ◽  
Gaba Accumulation

This study investigated the accumulation of γ-aminobutyric acid (GABA) in mung bean sprouts (Vigna Radiata L.) during germination under hypoxia/anaerobic conditions. Raw mung bean seeds were soaked in water at 35oC for 8 hours, dried and germinated under aerobic or hypoxia/anaerobic conditions at 35oC or 40oC for 0, 6, 12, 24, 36 and 48 hours.  Free amino acid and GABA contents were measured during the course of germination. Effect of soaking pH (5.3, 6.3 and 7.3) on GABA content of germinated mung beans under hypoxia/anaerobic condition was also investigated. The other objective of this study was to optimize the culture conditions of GABA production in germinated mung bean. A significant increase (p < 0.05) of GABA content was found in mung bean samples germinated under hypoxia/anaerobic condition. As this result, the combination of soaking pH 6.3 and 12 hour germination at 35oC was found to yield the highest GABA content (1519.07±19.58ppm).  This figure was 1.41 times as high as the content in aerobic condition and 25.32 times as high as the analysis of the raw mung beans. And experimental analysis showed that the optimal condition with aeration treatment for GABA accumulation in germinated mung bean was at a temperature of 36.6oC, time of 14.5 h and a pH value of 5.83; the highest GABA yield was 1638.67ppm, which was 27.77 times higher than raw seeds. In conclusion, germinated mung bean is a good source of GABA and hypoxia/anaerobic condition in combination with soaking pH, germination time and temperature can increase significantly GABA content in germinated mung beans.

Combining the biological nitrogen and sulfur cycles in anaerobic conditions

2001 ◽  
Vol 44 (8) ◽  
pp. 77-84 ◽  
Author(s):  
F. Fdz-Polanco ◽  
M. Fdz-Polanco ◽  
N. Fernández ◽  
M.A. Urueña ◽  
P.A. García ◽  
...  
Keyword(s):  
Electron Donor ◽  
Electron Acceptor ◽  
Sulfur Compounds ◽  
Ammonium Oxidation ◽  
Anaerobic Conditions ◽  
Reduced Sulfur Compounds ◽  
Gas Phases ◽  
Lack Of Information ◽  
Oxidation Reduction ◽  
Reduced Sulfur

The biochemical processes involved in the anaerobic degradation of carbon, nitrogen and sulfur compounds can be represented by an oxidation-reduction or electron donor-acceptor scheme. The theoretic values of Gibbs free energy (ΔG0) calculated from thermodynamic data indicate the feasibility of the reactions. The interactions C-S and C-N are well known but there is a lack of information about the interaction N-S. The anaerobic transformation of nitrates using reduced sulfur compounds can be explained considering that nitrate acts as electron acceptor while reduced sulfur compounds are the electron donors. A new N-S interaction in anaerobic conditions (ORP = -425 mV) has been experimentally observed when treating industrial wastewater rich in organic nitrogen and sulfate. The mass balances of the different nitrogenous and sulfur compounds in the liquid and gas phases clearly indicated an uncommon evolution. An important percentage of the nitrogen entering the reactor as TKN was removed from the liquid phase appearing as N2 in the gas phase. Simultaneously, only part of the sulfate initially present in the influent appeared as sulfide in the effluent or as hydrogen sulfide in the gas. These experimental observations may suggest a new anaerobic N-S biological interaction involving simultaneous anaerobic ammonium oxidation and sulfate reduction, ammonium being the electron donor and sulfate the electron acceptor.

scholarly journals Iron Corrosion Induced by Nonhydrogenotrophic Nitrate-Reducing Prolixibacter sp. Strain MIC1-1

2014 ◽  
Vol 81 (5) ◽  
pp. 1839-1846 ◽  
Author(s):  
Takao Iino ◽  
Kimio Ito ◽  
Satoshi Wakai ◽  
Hirohito Tsurumaru ◽  
Moriya Ohkuma ◽  
...  
Keyword(s):  
Carbon Source ◽  
Electron Donor ◽  
Microbiologically Influenced Corrosion ◽  
Rrna Gene ◽  
Oil Well ◽  
Metallic Materials ◽  
Anaerobic Conditions ◽  
Iron Corrosion ◽  
Content Type ◽  
Donor Acceptor

ABSTRACTMicrobiologically influenced corrosion (MIC) of metallic materials imposes a heavy economic burden. The mechanism of MIC of metallic iron (Fe0) under anaerobic conditions is usually explained as the consumption of cathodic hydrogen by hydrogenotrophic microorganisms that accelerates anodic Fe0oxidation. In this study, we describe Fe0corrosion induced by a nonhydrogenotrophic nitrate-reducing bacterium called MIC1-1, which was isolated from a crude-oil sample collected at an oil well in Akita, Japan. This strain requires specific electron donor-acceptor combinations and an organic carbon source to grow. For example, the strain grew anaerobically on nitrate as a sole electron acceptor with pyruvate as a carbon source and Fe0as the sole electron donor. In addition, ferrous ion andl-cysteine served as electron donors, whereas molecular hydrogen did not. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain MIC1-1 was a member of the genusProlixibacterin the orderBacteroidales. Thus,Prolixibactersp. strain MIC1-1 is the first Fe0-corroding representative belonging to the phylumBacteroidetes. Under anaerobic conditions,Prolixibactersp. MIC1-1 corroded Fe0concomitantly with nitrate reduction, and the amount of iron dissolved by the strain was six times higher than that in an aseptic control. Scanning electron microscopy analyses revealed that microscopic crystals of FePO4developed on the surface of the Fe0foils, and a layer of FeCO3covered the FePO4crystals. We propose that cells ofProlixibactersp. MIC1-1 accept electrons directly from Fe0to reduce nitrate.

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