Phosphorus uptake by Oscillatoria sp. microalgae for bioavailability under anaerobic condition to predict eutrophication

2021 ◽  
Vol 25 (11) ◽  
pp. 153-156
Author(s):  
Askal Maimulyanti
Keyword(s):  
Linear Correlation ◽  
Incubation Time ◽  
Anaerobic Condition ◽  
Water Solution ◽  
Phosphorus Uptake ◽  
Phosphate Concentration ◽  
Maximum Efficiency ◽  
Anaerobic Conditions ◽  
Algae Growth ◽  
Limiting Nutrient

Phosphorus is generally considered the limiting nutrient for algae growth in the aquatic environment. The phosphorus uptake for algae can give information for bioavailability-P. The contribution of phosphorous to bioavailability was evaluated using uptake-P in water solution under anaerobic conditions. The more is the incubation time, the more is the phosphate bioavailability. The maximum bioavailability occurred at 14 days with a phosphate concentration of 50 ppm resulting in P-available of 18.41 ppm. There was a linear correlation between P-available with P-medium with R2 = 0.97. The phosphorus bioavailability can be resulted from the equation: [P-Available] = 0.3789 x [P-medium]. The phosphate absorbed by the algae Oscillatoria sp in anaerobic conditions with initial concentrations of 10, 20, 30, 40 and 50 ppm was 5.46, 8.65, 11.63, 17.99 and 18.41 ppm, respectively. The maximum efficiency adsorption occurred in the concentration of 30 ppm with 49.18% adsorption.

Oxygen concentration affects frequency and range of transconjugants for the incompatibility (Inc) P-1 and P-7 plasmids pBP136 and pCAR1

2020 ◽  
Vol 85 (4) ◽  
pp. 1005-1015
Author(s):  
Kentaro Ochi ◽  
Maho Tokuda ◽  
Kosuke Yanagiya ◽  
Chiho Suzuki-Minakuchi ◽  
Hideaki Nojiri ◽  
...  
Keyword(s):  
Oxygen Concentration ◽  
Anaerobic Condition ◽  
Environmental Samples ◽  
Aerobic Condition ◽  
Recipient Strain ◽  
Donor Strain ◽  
Anaerobic Conditions ◽  
Filter Mating ◽  
Aerobic And Anaerobic ◽  
Aerobic And Anaerobic Conditions

ABSTRACT The frequency of transconjugants were compared for the incompatibility (Inc) P-1 and P-7 plasmids pBP136 and pCAR1 under aerobic and anaerobic conditions. Filter mating assays were performed with one donor strain and one recipient strain using different donors of Pseudomonas and recipient strains, including Pseudomonas, Pantoea, and Buttiauxella. Under anaerobic condition, frequencies of transconjugants for both plasmids were 101-103-fold lower than those under aerobic condition regardless of whether aerobically or anaerobically grown donors and recipients were used. To compare the transconjugant ranges under aerobic and anaerobic conditions, conjugation was performed between the donor of pBP136 and recipient bacteria extracted from environmental samples. Several transconjugants were uniquely obtained from each aerobic or anaerobic condition. Our findings indicate that a plasmid can differently spread among bacteria depending on the oxygen concentrations of the environment.

Influence of Different Salts on the Degradation of Trichloroethylene under Reductive Condition

2011 ◽  
Vol 356-360 ◽  
pp. 1689-1692
Author(s):  
Feng Na Ma ◽  
Qing Jun Meng ◽  
Guo Jun Wu ◽  
Ai Qin Shan
Keyword(s):  
Electron Donor ◽  
Anaerobic Condition ◽  
Anoxic Condition ◽  
Trivalent Iron ◽  
Anaerobic Conditions ◽  
Iron Salts ◽  
Anoxic Environment ◽  
Mixed Salts ◽  
Tce Degradation

The effect of TCE cometabolism degradation with glucose as electron donor on reduction progress was investigated with enriched nitrate, sulfate, trivalent iron and their mixtures under anoxic or anaerobic conditions. The removal effect of TCE was found to be better in anoxic environment; TCE degradation was inhibited by the addition of trivalent iron salts and the mixed salts under anoxic condition or strongly inhibited by nitrate under anaerobic condition; promoted by the addition of trivalent iron salts under anaerobic condition; little inhibited by the addition of nitrate and sulfate under anoxic condition or sulfate and the mixed salts under anaerobic condition.

scholarly journals Incubation Studies on Exchangable Zn for Varying Levels of added Zn Under Aerobic and Anaerobic Conditions in Grey Terrace Soils, Non Calcarious Floodplain Soils and Calcarious Floodplain Soils

2013 ◽  
Vol 9 (1-2) ◽  
pp. 9-15 ◽  
Author(s):  
KH Talukder ◽  
IU Ahmed ◽  
MS Islam ◽  
MS Islam ◽  
M Asaduzzaman ◽  
...  
Keyword(s):  
Anaerobic Condition ◽  
Early Stage ◽  
Aerobic Condition ◽  
Anaerobic Conditions ◽  
Floodplain Soil ◽  
Zinc Fractions ◽  
Floodplain Soils ◽  
Soil Zn ◽  
Aerobic And Anaerobic ◽  
Aerobic And Anaerobic Conditions

Fractions studies were done to know how the zinc applied to different soils was distributed in to various fractions when the soils incubated under aerobic and anaerobic condition. The added zinc provided significant increase in exchangeable Zn both under aerobic and anaerobic conditions although anaerobic condition gave lower results than aerobic condition. The higher results were obtained at early stage of incubation and it gradually reduced as the incubation period proceeded to 90 days. These results showed all most similar trends for all the soils under study. In general, added zinc showed significantly higher results to the different fractions of soil Zn both under anaerobic and aerobic incubation with very few exceptions. The highest amount of added Zn (12 kg/ha) always produced greater results than the lower doses. Only exchangeable Zn was found higher in the 1st measurement at 15 DAI then gradually decreased but in other cases, gradual increase in zinc fractions was seen as the incubation study proceed to longer duration provided with very few exceptions. In many cases, the exchangeable-Zn found higher only at 15 DAI but sharply reduced at 30 DAI. In general, the Gray Terrace Soil produced the highest results followed by Non Calcareous Gray Floodplain and the lowest results were observed in Dark Grey Floodplain & Brown Floodplain Soil. DOI: http://dx.doi.org/10.3329/jsf.v9i1-2.14643 J. Sci. Foundation, 9(1&2): 9-15, June-December 2011

Assessment of Anaerobic Culture Incubation Time: A Tertiary Care Center Experience

2019 ◽  
Vol 152 (Supplement_1) ◽  
pp. S132-S133
Author(s):  
Akanksha Gupta ◽  
Arti Easwar ◽  
Yanice Maldonado ◽  
Pam Hamilton ◽  
Lori Baumgartner ◽  
...  
Keyword(s):  
Incubation Time ◽  
Trauma Center ◽  
Care Center ◽  
Tertiary Care ◽  
Anaerobic Culture ◽  
Anaerobic Conditions ◽  
Gram Positive ◽  
Gram Negative ◽  
Level I ◽  
The Ideal

Abstract Background Currently, there is no consensus on the ideal incubation time for anaerobic cultures. A recent survey of CliniMicroNet reported anaerobic culture incubation times from 2 to 7 days. The goal of this study is to determine the ideal anaerobic culture incubation time and to retrospectively determine if longer incubation time affected management. Methods In this prospective study at Hartford Hospital, an 869-bed level I trauma center, 838 consecutive anaerobic cultures were Gram stained and planted on anaerobic blood, colistin and nalidixic acid (CNA), and kanamycin and vancomycin (KV) agar plates (BD Diagnostic Sparks). Plates were incubated in jars at 35°C and Anoxomat system (Advanced Instruments) was used to create anaerobic conditions. Plates were checked for growth on days 2, 3, 5, and 7. Although not all patient records (EPIC) were available, the type and duration of antibiotics were recorded for more than 100 records. Results Out of 74 cultures from bronchoalveolar lavage (1), bone (2), fluid (21), tissue (14), and wound (36), 53 grew 1 isolate, 15 grew 2 isolates, 5 grew 3 isolates, and 1 grew 4 isolates, for a total of 102 isolates. Sixty percent of isolates were Gram negative and 40% were Gram positive. Of these, 43% were detected on day 2, 21% on day 3, 20% on day 5, and 17% on day 7. In a majority of cases, broad-spectrum antibiotics (vancomycin/cefepime) were administered until culture results were obtained. Conclusion Overall, 73% Gram-negative isolates and 50% Gram-positive isolates were detected on day 2 or 3. Days 5 and 7 yielded 20% and 17% of all isolates, respectively. We conclude and corroborate with CLSI recommendations that anaerobic cultures should be incubated for at least 5 to 7 days.

MICROAEROPHILIC AND ANAEROBIC DECOLORIZATION OF AZO DYE IN RICH AND MINIMAL MEDIA BY CITROBACTER SP. STRAIN L17

2017 ◽  
Vol 79 (4) ◽  
Author(s):  
Ali Hossennia ◽  
Giek Far Chan ◽  
Noor Aini Abdul Rashid ◽  
Mohd Firdaus Abdul-Wahab
Keyword(s):  
Anaerobic Condition ◽  
Azo Dye ◽  
Biogas Production ◽  
Rich Medium ◽  
Anaerobic Conditions ◽  
Microaerophilic Condition ◽  
Degrading Bacteria ◽  
Different Temperatures ◽  
Minimal Media ◽  
Anaerobic Decolorization

This study aimed to investigate the ability of Citrobacter sp. strain L17 to decolourise azo dye in a rich medium (MP5) and three different minimal media (MMP5, MMGF11 and MM63) under microaerophilic and anaerobic conditions. Amaranth was used as the model dye in this investigation. Under microaerophilic condition, reactions were carried out at two different temperatures; 37°C and 45°C, whereas experiments with anaerobic condition were conducted only at 37°C. Results showed that, under microaerophilic condition, full decolourisation of Amaranth was achieved in all media tested at 37°C. However, at 45°C, complete decolourisation was observed only in MP5, MM63 and MMGF11 but no obvious decolourisation occurred in MMP5. On the other hand, complete decolourisation was observed in all media tested under anaerobic condition at 37°C, with the fastest decolourisation in MP5 (rich medium containing glucose and nutrient broth) and the slowest in MMP5. It was found that an inorganic buffer containing glucose at lower concentration was sufficient to achieve complete decolourisation under anaerobic condition. This finding is essential to identify a suitable medium for future study on biogas production by dye-degrading bacteria, which mostly requires anaerobic conditions.

scholarly journals Effects of phosphorus on the growth and chlorophyll fluorescence of a Dunaliella salina strain isolated from saline soil under nitrate limitation

2016 ◽  
Vol 89 (2) ◽  
Author(s):  
Tassnapa Wongsnansilp ◽  
Niran Juntawong ◽  
Zhe Wu
Keyword(s):  
Dunaliella Salina ◽  
Beta Carotene ◽  
Phosphate Concentration ◽  
Carotene Content ◽  
Ps Ii ◽  
Algae Growth ◽  
Kinetics Of ◽  
Late Growth ◽  
High Phosphate ◽  
Northeastern Thailand

An isolated <em>Dunaliella salina</em> (<em>D. salina</em>) KU XI from saline soils in northeastern Thailand was cultured in f/2 medium in column photobioreactor. The variations of the growth, chlorophyll and beta-carotene content and the maximum quantum yield of PS II photochemistry (F<sub>v</sub>/F<sub>m</sub>) under different NaH<sub>2</sub>PO<sub>4</sub> concentrations were studied. Based on the results, the growth kinetics of <em>D. salina</em> KU XI was established, which could simulate the algae growth rate under different phosphate concentrations and temperatures. The phosphorus could significantly affect the growth and pigments accumulations of this isolated strain. Increasing NaH<sub>2</sub>PO<sub>4</sub> concentration improved the biomass, the total chlorophyll and beta-carotene content, retarded the decrease of F<sub>v</sub>/F<sub>m</sub> value. The optimal phosphate concentration for the growth of <em>D. salina</em> KU XI was above 72.6 μM. The maximum biomass and beta-carotene were 0.24 g L<sup>-1</sup> and 17.4 mg L<sup>-1</sup> respectively when NaH<sub>2</sub>PO<sub>4</sub> was 290.4 μM. The algae growth was restrained by phosphate or nitrate when NaH<sub>2</sub>PO<sub>4</sub> below 12.1 μM or above 72.6 μM. It indicated that properly supplementing nitrate in the late growth stage with high phosphate concentration was favored for enhancing the growth and biomass production.

The effects of phosphate on physiological responses and carbohydrate production in Ulva fasciata (Chlorophyta) from upwelling and non-upwelling sites

2021 ◽  
Vol 64 (1) ◽  
pp. 1-11
Author(s):  
Tiphane Andrade Figueira ◽  
Nuno Tavares Martins ◽  
Lígia Ayres-Ostrock ◽  
Estela M. Plastino ◽  
Alex Enrich-Prast ◽  
...  
Keyword(s):  
Physiological Responses ◽  
Phosphorus Uptake ◽  
Experimental Period ◽  
Dry Mass ◽  
Carbohydrate Content ◽  
Phosphate Concentration ◽  
Nutrient Concentrations ◽  
Progressive Decline ◽  
Ulva Fasciata ◽  
Carbohydrate Contents

Abstract Phosphorus is a key macronutrient in macroalgal physiology, including carbohydrate anabolism. To determine the effects of phosphorus on different physiological parameters, we cultivated Ulva fasciata specimens from distinct localities (upwelling and non-upwelling sites) in the presence of different phosphate concentrations (0, 2, and 4 µM PO 4 3 − ${\text{PO}}_{4}^{3-}$ ). After 15 days, growth rates were similar (approx. 12% day−1) and carbohydrate contents had increased in individuals from both sites. In individuals from the upwelling site, carbohydrate contents were high in all treatments (71% dry mass), whereas non-upwelling site individuals cultivated under the highest phosphate concentration showed the lowest carbohydrate content (46% DM). Nevertheless, we observed higher rates of phosphorus uptake in individuals from the non-upwelling site cultivated under the highest phosphate concentration, indicating a stress response to elevated nutrient concentrations. Individuals from both sites cultivated with phosphate maintained healthy photosystems over the experimental period (F v/F m = 0.788), whereas those cultivated in the absence of phosphate showed evidence of photosystem impairment, as indicated by a progressive decline in maximum quantum yield. Altogether, our results indicate that site origin and phosphate concentration influence the carbohydrate content in U. fasciata and that individuals from sites with broad environmental variation, such as upwelling events, can show higher productivity.

scholarly journals Abundance and characterization of microorganisms isolated from oil palm empty fruit bunches waste under aerobic, anaerobic, and facultative anaerobic conditions

2020 ◽  
Vol 21 (9) ◽  
Author(s):  
DERMIYATI DERMIYATI ◽  
RADIX SUHARJO ◽  
MARELI TELAUMBANUA ◽  
RULLY YOSITA ◽  
ANGGI WINANDA SARI ◽  
...  
Keyword(s):  
Oil Palm ◽  
Anaerobic Condition ◽  
Bacterial Abundance ◽  
Plate Count ◽  
Cellulose Content ◽  
Anaerobic Conditions ◽  
Empty Fruit Bunches ◽  
Facultative Anaerobic ◽  
Different Characteristics

Abstract. Dermiyati, Suharjo R, Telaumbanua M, Yosita R, Sari AW, Andayani AP. 2020. Abundance and characterization of microorganisms isolated from oil palm empty fruit bunches waste under aerobic, anaerobic, and facultative anaerobic conditions. Biodiversitas 21: 4213-4220. Increased production of palm oil causes an increase in oil palm empty fruit bunches waste, while the utilization of the waste has not been carried out optimally. Oil palm empty fruit bunches contain high cellulose content which promotes the development of various types of bacteria with different characteristics. This study was aimed to determine the abundance and the characterization of bacteria that were isolated from extract suspension of oil palm empty fruit bunches in the form of a local microorganism solution under aerobic, anaerobic, and facultative anaerobic conditions. For isolation, the oil palm empty fruit extract suspension was inoculated on media Plate Count Agar Peptone and bacterial abundance was determined. Various bacterial characterization test included bacterial morphology test in the form of color and shape of macroscopic isolates, gram test using KOH 3%, fermentative oxidative test, soft rot test on potato tubers, hypersensitivity test on tobacco plant leaves, and hypo virulent test which was indicated by the presence of symptoms of disease in cucumber sprouts and indicated in the disease severity index (DSI) was carried out. The results of local microorganism solution isolation from oil palm empty fruit bunches obtained as many as 220 bacterial isolates consisting of 84 isolates in aerobic condition, 68 isolates in anaerobic condition, and 68 isolates in facultative anaerobic condition. The highest bacterial abundance in aerobic conditions was equal to 285.60 x 1010 CFU mL-1. Each bacterial isolate had different characteristics. Most of the characteristics of bacteria were turbid white (34.55%), round (69.55%), gram-positive (76.36%), fermentative (89.55%), softrot negative (71.82%), hypersensitive negative (97.27%), and virulent (74.55%).

scholarly journals EFFECTS OF PH SOAKING SOLUTIONS AND HYPOXIA/ANAEROBIC TREAMENT ON GABA ACCUMULATION IN GERMINATED MUNG BEAN (VIGNA RADIATA L.)

2017 ◽  
Vol 55 (2) ◽  
pp. 150 ◽  
Author(s):  
Trung Nhat Truong ◽  
Nguyen Tuong Danh ◽  
Dong Thi Anh Dao
Keyword(s):  
Anaerobic Condition ◽  
Vigna Radiata ◽  
Mung Bean ◽  
Ph Value ◽  
Aerobic Condition ◽  
Culture Conditions ◽  
Anaerobic Conditions ◽  
Gaba Production ◽  
Gaba Content ◽  
Gaba Accumulation

This study investigated the accumulation of γ-aminobutyric acid (GABA) in mung bean sprouts (Vigna Radiata L.) during germination under hypoxia/anaerobic conditions. Raw mung bean seeds were soaked in water at 35oC for 8 hours, dried and germinated under aerobic or hypoxia/anaerobic conditions at 35oC or 40oC for 0, 6, 12, 24, 36 and 48 hours.  Free amino acid and GABA contents were measured during the course of germination. Effect of soaking pH (5.3, 6.3 and 7.3) on GABA content of germinated mung beans under hypoxia/anaerobic condition was also investigated. The other objective of this study was to optimize the culture conditions of GABA production in germinated mung bean. A significant increase (p < 0.05) of GABA content was found in mung bean samples germinated under hypoxia/anaerobic condition. As this result, the combination of soaking pH 6.3 and 12 hour germination at 35oC was found to yield the highest GABA content (1519.07±19.58ppm).  This figure was 1.41 times as high as the content in aerobic condition and 25.32 times as high as the analysis of the raw mung beans. And experimental analysis showed that the optimal condition with aeration treatment for GABA accumulation in germinated mung bean was at a temperature of 36.6oC, time of 14.5 h and a pH value of 5.83; the highest GABA yield was 1638.67ppm, which was 27.77 times higher than raw seeds. In conclusion, germinated mung bean is a good source of GABA and hypoxia/anaerobic condition in combination with soaking pH, germination time and temperature can increase significantly GABA content in germinated mung beans.

Development of 2-reactor intermittent-aeration activated sludge process for simultaneous removal of nitrogen and phosphorus

1996 ◽  
Vol 34 (1-2) ◽  
pp. 111-118 ◽  
Author(s):  
Kousei Sasaki ◽  
Yasuji Yamamoto ◽  
Kazushi Tsumura ◽  
Sachiko Ouchi ◽  
Yutaka Mori
Keyword(s):  
Activated Sludge ◽  
Phosphorus Uptake ◽  
Phosphorus Release ◽  
Activated Sludge Process ◽  
Intermittent Aeration ◽  
Anaerobic Conditions ◽  
Nitrogen And Phosphorus ◽  
In Series ◽  
Plant Experiment ◽  
One Year

We have developed a new intermittently aerated anaerobic-aerobic activated sludge process. This process employs 2 reactors connected in series, and is capable of controlling the duration of aerobic, anoxic and anaerobic conditions in both reactors by utilizing the ORP bending point, which corresponds to the termination of denitrification. One cycle of aeration and agitation takes approximately 120 minutes. In the 1st reactor, nitrification and phosphorus uptake occur in the aeration period, followed by denitrification and phosphorus release in the agitation period. In the 2nd reactor, nitrification and phosphorus uptake are conducted during aeration, and denitrification and weak phosphorus uptake during agitation. A one-year pilot plant experiment using sewage (22 m3/day) showed stable and high removal ratios of 98% for BOD, 92% for T-N, and 85% for T-P under conditions of HRT 20 hours and water temperature 9∼33°C.

Sign in / Sign up

Export Citation Format

Share Document