Optimization of Liquid Fermentation for Mycelial Growth and Extracellular Polysaccharide Production by Poria cosos

2012 ◽  
Vol 554-556 ◽  
pp. 962-968
Author(s):  
Shuang Niu ◽  
Li Min Hao ◽  
Shu Xin Zhao ◽  
Fei Fei Wang ◽  
Chun Hui Du
Keyword(s):  
Mycelial Growth ◽  
Ph Value ◽  
Extracellular Polysaccharide ◽  
Liquid Volume ◽  
Growth Line ◽  
Flask Culture ◽  
Culture Experiment ◽  
Fermentation Conditions ◽  
Exopolysaccharide Production ◽  
Initial Ph

This paper is an attempt to study the growth line of poria cocos through flask culture experiment, and leads to a discussion of different fermentation conditions. The optimum media for mycelial growth were determined as (g/l): glucose 60, peptone 10, KNO310, KH2PO43, and MgSO41; while for exopolysaccharide was (g/l), glucose 40, peptone 5, KNO35, KH2PO40.5, MgSO41. The optimum initial pH value of medium was 5.5, inoculation, 10% (v/v) and different liquid volume had minimal influence on the mycelial growth and exopolysaccharide production.

Optimization of Fermentation Conditions for Synthesis Dextran by Biochemical Conversion

2011 ◽  
Vol 396-398 ◽  
pp. 1611-1614
Author(s):  
Ming Zheng Liang ◽  
Mei Li ◽  
Li Hong Lan ◽  
Tao Xie ◽  
Lei Zhong ◽  
...  
Keyword(s):  
Leuconostoc Mesenteroides ◽  
Rotation Speed ◽  
Ph Value ◽  
Shake Flask Culture ◽  
Flask Culture ◽  
Inoculum Concentration ◽  
Fermentation Conditions ◽  
Yield Rate ◽  
Initial Ph ◽  
Optimization Of Fermentation

To improve the production of dextran, fermentation conditions for cultivation of an Leuconostoc mesenteroides CICC-21725 were investigated. The fermentation conditions were optimized preliminarily by using the method of univariate experiment in the shake-flask culture, and the yield rate of dextran was used as the investigation index. The result shows that when the fermentation temperature, initial pH value, rotation speed and inoculum concentration were 25 °C, 7.5 , 0 r/min and 10%, respectively, the yield rate of dextran was the highest. Under the optimal fermentation conditions, the yield rate is approximately 98%, which is about 12% higher than the unoptimized.

scholarly journals Optimization of Culture Conditions for Amoxicillin Degrading Bacteria Screened from Pig Manure

2020 ◽  
Vol 17 (6) ◽  
pp. 1973 ◽  
Author(s):  
Xuanjiang Yang ◽  
Panpan Guo ◽  
Miao Li ◽  
Hualong Li ◽  
Zelin Hu ◽  
...  
Keyword(s):  
Response Surface ◽  
Ph Value ◽  
Culture Conditions ◽  
Pig Manure ◽  
Bacterial Strains ◽  
Inoculum Level ◽  
Fermentation Conditions ◽  
Culture Time ◽  
Degrading Bacteria ◽  
Initial Ph

(1) Objective: The objective of this study was to screen amoxicillin (AMX)-degrading bacterial strains in pig manure and optimize the fermentation conditions for these strains to achieve high fermentation rate, which can provide an effective way for the practical application of bacterial strains as antibiotic-degrading bacterial in treating livestock waste for antibiotic residues. (2) Methods: Antibiotic susceptibility tests and high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) were employed to screen AMX-degrading bacterial strains in pig manure. The culture conditions were optimized for AMX-degrading bacterial strains using Plackeet–Burman design (PBD), the steepest ascent design, and the response surface methods, coupled with the Box–Behnken design (BBD). The effects of culture time, temperature, rotator (mixing) speed, inoculum level, and initial pH value on the growth of AMX-degrading strains were investigated. Experimental data obtained from BBD were utilized to generate a second-order polynomial regression model for evaluating the effects of the tested variables on the optical density at 600 nm (OD600) of culture solutions as the growth indicator for the screened AMX-degrading strains. (3) Results: The initial pH, culture time, and the inoculum level had significant effects on the OD600 value (growth) of the screened AMX-degrading strains. The initial pH value was found to be the most critical factor influencing the growth of bacteria. The optimized culture condition for the bacterial growth determined by the response surface methodology was: the initial pH of 6.9, culture time of 52 h, and inoculum level of 2%. The average OD value of 12 different fermentation conditions in the initial fermentation tests in this study was 1.72 and the optimization resulted in an OD value of 3.00. The verification experiment resulted in an OD value of 2.94, which confirmed the adequacy of the optimization model for the determining the optimal culture condition. (4) Conclusions: The growth of the screened strain of AMX-degrading bacteria could be optimized by changing the fermentation conditions. The optimization could be achieved by using the Box–Behnken response surface method and Plackett–Burman experimental design.

Improvement of Shake Flask Fermentation Conditions for the Pseudomonas Strain N1

2011 ◽  
Vol 340 ◽  
pp. 305-310
Author(s):  
Wen Yi Zhang ◽  
Rong Chen ◽  
Li Qiao Lu ◽  
Xia Xu ◽  
Suo Hua Wu
Keyword(s):  
Shake Flask ◽  
Culture Medium ◽  
Ph Value ◽  
Cell Concentration ◽  
Sodium Succinate ◽  
Simultaneous Nitrification And Denitrification ◽  
Fermentation Conditions ◽  
Initial Ph ◽  
The Common ◽  
Shake Flask Fermentation

To improve the yield of the Pseudomonas strain N1 in the fermentation culture medium screened from the biofilm of BAF reactor, the optimal shake flask fermentation conditions for Pseudomonas N1 were determined by single factor test and orthogonal test. The composition of optimal fermentation culture medium was as follows: nitrate of potash of 4 g/L, sodium succinate of 26.1 g/L, KH2PO4 of 4.0 g/L, MgSO4·7H2O of 1 g/L, KCl of 1 g/L. The cell concen- tration could reach 9.08 g/L under the conditions. Fermentation was performed for 36h in a 250ml shake flask containing 70mL medium at 1% inoculums volume and initial pH value 7.0. The cell concentration could reach 12.86 g/L which was 2.5 fold higher than that under the original conditions. Simultaneous nitrification and denitrification could be achieved to alleviate the pollution of nitrogen in the wastewater by adding the microbial inoculums into the common aerobic nitrifica- tion sludge.

scholarly journals Increasing the Production of Xenocoumacin 1 by Optimizing the Fermentation Process of Xenorhabdus Nematophila

2021 ◽  
Author(s):  
Yunfei Han ◽  
Jiangtao Gao ◽  
Shujing Zhang ◽  
Jinhua Han ◽  
Zhiqiang Yan ◽  
...  
Keyword(s):  
Fermentation Process ◽  
Inoculum Size ◽  
Water Soluble ◽  
Liquid Volume ◽  
Agitation Rate ◽  
Xenorhabdus Nematophila ◽  
Fermentation Conditions ◽  
Medium Components ◽  
Proteose Peptone ◽  
Initial Ph

Abstract Background: Xenocoumacin 1 (Xcn 1), a kind of water-soluble antibiotic discovered from the cell-free broth of Xenorhabdus nematophila YL001, has exhibited excellent activity against bacteria, oomycetes and fungi. However, the low yield limits the development and utilization of Xcn1. In order to increase the yield of Xcn1, the fermentation process was optimized in this study. Results: Maltose and proteose peptone were identified as the best carbon and nitrogen sources that significantly affected Xcn1 production using a-factor-at-a-time approach. Response surface methodology was applied to optimize the medium constituents for Xcn1 production by X. nematophila YL001. Higher Xcn1-content (113.65 μg/mL) was obtained after optimizing medium components. The optimal levels of medium components were (g/L): proteose peptone 20.83, maltose 12.74, K2HPO4 3.77. Fermentation conditions, such as initial pH, inoculum size, temperature, rotation speed, liquid volume and the length of fermentation, were also investigated by using a-factor-at-a-time method to get a higher production of Xcn1. X. nematophila YL001 was able to produce higher Xcn1 (153.56 μg/mL) at 25°C, initial pH 7.0, inoculum size 10%, culture medium 75 mL in a 250 mL shake flash with an agitation rate of 150 rpm for 48h. Additionally, kinds, concentrations and adding time of the precursor were also investigated. X. nematophila YL001 was able to produce the highest Xcn1 (173.99 μg/mL) when the arginine was added to the broth with 3 mmol/L at the 12th hour. An overall 243.38% increase in Xcn1 content was obtained as compared with mean observed response at TSB medium.Conclusions: To the best of our knowledge, there are no reports on optimization of fermentation process for Xcn1 production quantified by HPLC. The results show that nutrition, precursors and fermentation conditions had a highly influence on the production of Xcn1 by X. nematophila YL001. The optimized medium and fermentation conditions resulted in a 243.38% increase in Xcn1 production. This work will be helpful for the development of X. nematophila YL001 cultivation process for efficient Xcn1 production and lay a foundation for its industrial production.

Optimization of Lipase Production from Arthrobacter sp.SD5

2013 ◽  
Vol 791-793 ◽  
pp. 116-119 ◽  
Author(s):  
Peng Ren ◽  
Wei Hua Lin
Keyword(s):  
Carbon Source ◽  
Culture Condition ◽  
Ph Value ◽  
Tween 80 ◽  
Lipase Production ◽  
Medium Composition ◽  
Liquid Volume ◽  
Optimum Conditions ◽  
Culture Temperature ◽  
Initial Ph

Lipase is a kind of important hydrolase. It was widely used in the fields of food, leather, detergent and pharmaceutical. The production of lipase from Arthrobacter sp. SD5 was studied in the present paper. The medium composition and culture condition were optimized in order to improve lipase production. The results showed the best optimum conditions were on the following: culture temperature (40°C); initial pH value (8.0); liquid volume (20%); carbon source (olive oil, 2.5%); nitrogen source (peptone, 1.0%); biosurfactant (Tween-80, 0.2%).

scholarly journals Hydrothermal Synthesis of Various Shape-Controlled Europium Hydroxides

Nanomaterials ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 529
Author(s):  
Hongjuan Zheng ◽  
Kongjun Zhu ◽  
Ayumu Onda ◽  
Kazumichi Yanagisawa
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Ph Value ◽  
Hydrothermal Temperature ◽  
X Ray ◽  
Hydrothermal Reactions ◽  
Starting Solution ◽  
Initial Ph ◽  
Shape Controlled ◽  
Scanning Electron

Eu(OH)3 with various shape-controlled morphologies and size, such as plate, rod, tube, prism and nanoparticles was successfully synthesized through simple hydrothermal reactions. The products were characterized by XRD (X-Ray Powder Diffraction), FE-SEM (Field Emission- Scanning Electron Microscopy) and TG (Thermogravimetry). The influence of the initial pH value of the starting solution and reaction temperature on the crystalline phase and morphology of the hydrothermal products was investigated. A possible formation process to control morphologies and size of europium products by changing the hydrothermal temperature and initial pH value of the starting solution was proposed.

scholarly journals The sorption of inorganic arsenic on modified sepiolite: Effect of hydrated iron(III)-oxide

2014 ◽  
Vol 79 (7) ◽  
pp. 815-828 ◽  
Author(s):  
Nikola Ilic ◽  
Slavica Lazarevic ◽  
Vladana Rajakovic-Ognjanovic ◽  
Ljubinka Rajakovic ◽  
Djordje Janackovic ◽  
...  
Keyword(s):  
Sorption Capacity ◽  
Arsenic Removal ◽  
Ph Value ◽  
Inorganic Arsenic ◽  
Arsenic Species ◽  
Deionized Water ◽  
Order Kinetic ◽  
Initial Ph ◽  
Order Kinetic Model ◽  
Pseudo Second Order

The sorption of inorganic arsenic species, As(III) and As(V), from water by sepiolite modified with hydrated iron(III) oxide was investigated at 25 ?C through batch studies. The influence of the initial pH value, the initial As concentrations, the contact time and types of water on the sorption capacity was investigated. Two types of water were used, deionized and groundwater. The maximal sorption capacity for As(III) from deionized water was observed at initial and final pH value 7.0, while the bonding of As(V) was observed to be almost pH independent for pH value in the range from 2.0 to 7.0, and the significant decrease in the sorption capacity was observed at pH values above 7.0. The sorption capacity at initial pH 7.0 was about 10 mg g?1 for As(III) and 4.2 mg g?1 for As(V) in deionized water. The capacity in groundwater was decreased by 40 % for As(III) and by 20 % for As(V). The Langmuir model and pseudo-second order kinetic model revealed good agreement with the experimental results. The results show that Fe(III)-modified sepiolite exhibits significant affinity for arsenic removal and it has a potential for the application in water purification processes.

Optimization of Fermentation Conditions for Blue Pigment Production by Bacterial Strain T2013

2014 ◽  
Vol 1073-1076 ◽  
pp. 286-291
Author(s):  
Yong Zuo ◽  
Peng Jiang ◽  
Xiao Long Wang ◽  
Bin Fu ◽  
Xiao Long Yang ◽  
...  
Keyword(s):  
Bacterial Strain ◽  
Orthogonal Test ◽  
Blue Pigment ◽  
Liquid Volume ◽  
Initial Value ◽  
Fermentation Time ◽  
Fermentation Conditions ◽  
Inoculum Volume ◽  
Optimization Of Fermentation ◽  
Single Factor Experiment

The fermentation conditions of Bacterial Strain T2013 were studied. The fermentation conditions of T2013 were investigated with single-factor experiment. In addition to, the fermentation conditions were optimized through the orthogonal test. The results showed the highest production of blue pigment was obtained on condition that fermentation time was 2d, the liquid volume was 50ml in the 250ml shake-flask, the inoculum volume was 2%, the initial value of pH was 7.6, fermentation temperature was 37°C and the speed was 150r/min.The fermentation conditions of Bacterial Strain T2013 were preliminarily determined,whichprovides a theoretical basis for the industrial production of blue pigment.

scholarly journals The Removal of Methylene Blue Dye from Aqueous Solutions Using Activated and Non-Activated Bentonites

2003 ◽  
Vol 21 (5) ◽  
pp. 451-462 ◽  
Author(s):  
Sameer Al-Asheh ◽  
Fawzi Banat ◽  
Leena Abu-Aitah
Keyword(s):  
Methylene Blue ◽  
Ph Value ◽  
Sodium Dodecyl ◽  
Freundlich Isotherm ◽  
Blue Dye ◽  
Batch Adsorption ◽  
Ionic Surfactant ◽  
Methylene Blue Dye ◽  
X Ray Diffraction ◽  
Initial Ph

An improvement in the adsorption capacity of naturally available bentonite towards water pollutants such as Methylene Blue dye (MBD) is certainly needed. For this purpose, sodium bentonite was activated by two methods: (1) treatment with sodium dodecyl sulphate (SDS) as an ionic surfactant and (2) thermal treatment in an oven operated at 850°C. Batch adsorption tests were carried out on removing MBD from aqueous solution using the above-mentioned bentonites. It was found that the effectiveness of bentonites towards MBD removal was in the following order: thermal-bentonite > SDS-bentonite > natural bentonite. X-Ray diffraction analysis showed that an increase in the microscopic bentonite platelets on treatment with SDS was the reason behind the higher uptake of MBD. An increase in sorbent concentration or initial pH value of the solutions resulted in a greater removal of MBD from the solution. An increase in temperature led to an increase in MBD uptake by the bentonites studied in this work. The Freundlich isotherm model was employed and found to represent the experimental data well.

High Cr(VI) Adsorption Performance of Coal-Based Activated Carbon in Aqueous Solution

2013 ◽  
Vol 798-799 ◽  
pp. 1123-1127
Author(s):  
Hua Lei Zhou ◽  
Qiong Qiong Zhu ◽  
Dong Hua Huang
Keyword(s):  
Aqueous Solution ◽  
Activated Carbon ◽  
Pore Structure ◽  
Ph Value ◽  
High Surface ◽  
High Surface Area ◽  
Koh Activation ◽  
Transmission Electron ◽  
Mesoporous Adsorbent ◽  
Initial Ph

The activated carbon with high surface area was prepared by KOH activation from anthracite and used as adsorbent for removal of Cr (VI) from aqueous solution. The pore structure and surface properties were characterized by N2 adsorption at 77K, transmission electron microscope (TEM) and Fourier transform infrared spectroscopy ( FTIR). Effect of pH and isotherms at different temperature were investigated. Results show that the prepared carbon is a microporous-and mesoporous-adsorbent with developed pore structure and abundant surface oxygen-containing groups. PH value of the solution plays key function on the adsorption. The chemical adsorption dominates the adsorption process. The activated carbon exhibits much higher Cr adsorption capacity than the commercial activated carbon at initial pH of ~3. The equilibrium adsorption data are fitted by both Freundlich model and Langmuir model well.

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