Osteogenesis and Degradability of Bioresorbable Biphasic Gypsum-Carbonated Apatite Granules for Bone Tissue Engineering

2016 ◽  
Vol 705 ◽  
pp. 297-303
Author(s):  
Shirin Ibrahim ◽  
Syazana Abu Bakar ◽  
Mohamad Azmirruddin Ahmad ◽  
Nurul Awanis Johan ◽  
Siti Farhana Hisham ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Proliferation ◽  
Weight Loss ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Bone Matrix ◽  
Apatite Formation ◽  
Potential Candidate ◽  
Alp Activity ◽  
Carbonated Apatite

Osteogenesis and degradability of bioresorbable biphasic gypsum-carbonated apatite granules (BPG) were investigated. Three different sizes of gypsum, 300-600 μm (small), 600-1000 μm (medium) and 1000-2000 μm (large), denoted as S, M and L respectively, were developed through the crushing and sieving method. Exposure of gypsum granules in carbonate and phosphate sources formed BPG through dissolution and precipitation mechanism. BPG was firstly examined by X-ray Diffractometer (XRD) and Fourier Transform Infrared Spectrometer (FTIR) to confirm its phase and chemical composition respectively. In-vitro cell proliferation, alkaline phosphatase (ALP) activity and adhesion of human osteoblast (hFOB) were investigated for osteogenesis evaluation. Degradability in phosphate buffer saline (PBS) was characterized by weight loss whereas apatite mineralization on the BPG surface was examined using Scanning Electron Microscope (SEM). BPG with 300-600 μm and 600-1000 μm enhanced osteogenic differentiation of hFOB and accelerated differentiation process better than 1000-2000 μm as indicated by cell proliferation and ALP activity. Good hFOB adhesion was observed on all BPG surfaces. The weight loss of L and M was 68% and 59%, respectively, which are higher than S at only 32%, indicating faster degradation of large BPG compared to smaller granules upon immersion for 35 days. This in turn, suggested the ionic dissolution of BPG which has contributed to the apatite formation on its surface. The results suggest, the BPG mimicked the bone matrix, exhibited good osteogenesis and degradability, which might be used as a potential candidate for bone tissue engineering.

scholarly journals A novel gelatin/chitooligosaccharide/demineralized bone matrix composite scaffold and periosteum-derived mesenchymal stem cells for bone tissue engineering

2021 ◽  
Vol 25 (1) ◽  
Author(s):  
Thakoon Thitiset ◽  
Siriporn Damrongsakkul ◽  
Supansa Yodmuang ◽  
Wilairat Leeanansaksiri ◽  
Jirun Apinun ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Formation ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Alp Activity

Abstract Background A novel biodegradable scaffold including gelatin (G), chitooligosaccharide (COS), and demineralized bone matrix (DBM) could play a significant part in bone tissue engineering. The present study aimed to investigate the biological characteristics of composite scaffolds in combination of G, COS, and DBM for in vitro cell culture and in vivo animal bioassays. Methods Three-dimensional scaffolds from the mixture of G, COS, and DBM were fabricated into 3 groups, namely, G, GC, and GCD using a lyophilization technique. The scaffolds were cultured with mesenchymal stem cells (MSCs) for 4 weeks to determine biological responses such as cell attachment and cell proliferation, alkaline phosphatase (ALP) activity, calcium deposition, cell morphology, and cell surface elemental composition. For the in vivo bioassay, G, GC, and GCD, acellular scaffolds were implanted subcutaneously in 8-week-old male Wistar rats for 4 weeks and 8 weeks. The explants were assessed for new bone formation using hematoxylin and eosin (H&E) staining and von Kossa staining. Results The MSCs could attach and proliferate on all three groups of scaffolds. Interestingly, the ALP activity of MSCs reached the greatest value on day 7 after cultured on the scaffolds, whereas the calcium assay displayed the highest level of calcium in MSCs on day 28. Furthermore, weight percentages of calcium and phosphorus on the surface of MSCs after cultivation on the GCD scaffolds increased when compared to those on other scaffolds. The scanning electron microscopy images showed that MSCs attached and proliferated on the scaffold surface thoroughly over the cultivation time. Mineral crystal aggregation was evident in GC and greatly in GCD scaffolds. H&E staining illustrated that G, GC, and GCD scaffolds displayed osteoid after 4 weeks of implantation and von Kossa staining confirmed the mineralization at 8 weeks in G, GC, and GCD scaffolds. Conclusion The MSCs cultured in GCD scaffolds revealed greater osteogenic differentiation than those cultured in G and GC scaffolds. Additionally, the G, GC, and GCD scaffolds could promote in vivo ectopic bone formation in rat model. The GCD scaffolds exhibited maximum osteoinductive capability compared with others and may be potentially used for bone regeneration.

Development of functionalized multi-walled carbon nanotube-based polysaccharide–hydroxyapatite scaffolds for bone tissue engineering

RSC Advances ◽  
2016 ◽  
Vol 6 (85) ◽  
pp. 82385-82393 ◽  
Author(s):  
R. Rajesh ◽  
Y. Dominic Ravichandran ◽  
M. Jeevan Kumar Reddy ◽  
Sung Hun Ryu ◽  
A. M. Shanmugharaj
Keyword(s):  
Tissue Engineering ◽  
Cell Proliferation ◽  
Carbon Nanotube ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Gellan Gum ◽  
Alp Activity ◽  
Multi Walled Carbon Nanotube ◽  
First Time

fMWCNT–amylopectin–HAP and fMWCNT–gellan gum–HAP were prepared and characterized and their in vitro cell proliferation and ALP activity were checked for the first time.

Osteon-mimetic 3D nanofibrous scaffold enhancing stem cell proliferation and osteogenic differentiation for bone regeneration

2022 ◽  
Author(s):  
Ting Song ◽  
Jianhua Zhou ◽  
Ming Shi ◽  
Liuyang Xuan ◽  
Huamin Jiang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Proliferation ◽  
Stem Cell ◽  
Bone Tissue Engineering ◽  
Bone Regeneration ◽  
Osteogenic Differentiation ◽  
Bone Tissue ◽  
Nanofibrous Scaffold ◽  
Hierarchical Microstructure ◽  
Stem Cell Proliferation

Scaffold microstructure is important for bone tissue engineering. Failure to synergistically imitate the hierarchical microstructure of bone component, such as osteon with concentric multilayers assembled by nanofibers, hindered the performance...

The effect of fiber size and pore size on cell proliferation and infiltration in PLLA scaffolds on bone tissue engineering

2016 ◽  
Vol 30 (10) ◽  
pp. 1545-1551 ◽  
Author(s):  
Xuejun Wang ◽  
Tao Lou ◽  
Wenhua Zhao ◽  
Guojun Song ◽  
Chunyao Li ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Proliferation ◽  
Bone Tissue Engineering ◽  
Pore Size ◽  
Bone Tissue ◽  
Fiber Size

scholarly journals Comparison of the Anabolic Effects of Reported Osteogenic Compounds on Human Mesenchymal Progenitor-Derived Osteoblasts

2020 ◽  
Vol 7 (1) ◽  
pp. 12 ◽  
Author(s):  
Robert Owen ◽  
Hossein Bahmaee ◽  
Frederik Claeyssens ◽  
Gwendolen C. Reilly
Keyword(s):  
Tissue Engineering ◽  
Bone Formation ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Lithium Chloride ◽  
Osteoporosis Treatment ◽  
Bone Morphogenetic Protein 2 ◽  
Calcium Deposition ◽  
Matrix Deposition ◽  
Alp Activity

There is variability in the reported effects of compounds on osteoblasts arising from differences in experimental design and choice of cell type/origin. This makes it difficult to discern a compound’s action outside its original study and compare efficacy between compounds. Here, we investigated five compounds frequently reported as anabolic for osteoblasts (17β-estradiol (oestrogen), icariin, lactoferrin, lithium chloride, and menaquinone-4 (MK-4)) on human mesenchymal progenitors to assess their potential for bone tissue engineering with the aim of identifying a potential alternative to expensive recombinant growth factors such as bone morphogenetic protein 2 (BMP-2). Experiments were performed using the same culture conditions to allow direct comparison. The concentrations of compounds spanned two orders of magnitude to encompass the reported efficacious range and were applied continuously for 22 days. The effects on the proliferation (resazurin reduction and DNA quantification), osteogenic differentiation (alkaline phosphatase (ALP) activity), and mineralised matrix deposition (calcium and collagen quantification) were assessed. Of these compounds, only 10 µM MK-4 stimulated a significant anabolic response with 50% greater calcium deposition. Oestrogen and icariin had no significant effects, with the exception of 1 µM icariin, which increased the metabolic activity on days 8 and 22. 1000 µg/mL of lactoferrin and 10 mM lithium chloride both significantly reduced the mineralised matrix deposition in comparison to the vehicle control, despite the ALP activity being higher in lithium chloride-treated cells at day 15. This demonstrates that MK-4 is the most powerful stimulant of bone formation in hES-MPs of the compounds investigated, highlighting its potential in bone tissue engineering as a method of promoting bone formation, as well as its prospective use as an osteoporosis treatment.

scholarly journals Enhanced Cell Proliferation and Osteogenesis Differentiation through a Combined Treatment of Poly-L-Lysine-Coated PLGA/Graphene Oxide Hybrid Fiber Matrices and Electrical Stimulation

2020 ◽  
Vol 2020 ◽  
pp. 1-15
Author(s):  
Jiaqi Zhu ◽  
Zhiping Qi ◽  
Changjun Zheng ◽  
Pan Xue ◽  
Chuan Fu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Proliferation ◽  
Graphene Oxide ◽  
Electrical Stimulation ◽  
Bone Tissue Engineering ◽  
Bone Regeneration ◽  
Bone Tissue ◽  
Cellular Response ◽  
Hybrid Fiber ◽  
Fiber Matrices

Bone tissue engineering scaffold provides an effective treatment for bone defect repair. Biodegradable bone scaffold made of various synthetic and natural materials can be used as bone substitutes and grafts for defect site, which has great potential to support bone regeneration. Regulation of cell-scaffold material interactions is an important factor for modulating the cellular activity in bone tissue engineering scaffold applications. Thus, the hydrophilic, mechanical, and chemical properties of scaffold materials directly affect the results of bone regeneration and functional recovery. In this study, a poly-L-lysine (PLL) surface-modified poly(lactic-co-glycolic acid) (PLGA)/graphene oxide (GO) (PLL-PLGA/GO) hybrid fiber matrix was fabricated for bone tissue regeneration. Characterization of the resultant hybrid fiber matrices was done using scanning electron microscopy (SEM), contact angle, and a material testing machine. According to the results obtained from the test above, the PLL-PLGA/GO hybrid fiber matrices exhibited high wettability and mechanical strength. The special surface characteristics of PLL-PLGA/GO hybrid fiber matrices were more beneficial for protein adsorption and inhibit the proliferation of pathogens. Moreover, the enhanced regulation of MC3T3-E1 cell proliferation and differentiation was observed, when the resultant hybrid fiber matrices were combined with electrical stimulation (ES). The cellular response of MC3T3-E1 cells including cell adhesion, proliferation, alkaline phosphatase (ALP) activity, calcium deposition, and osteogenesis-related gene expression was significantly enhanced with the synergistic effect of resultant hybrid fiber matrices and ES. These data indicate that the PLL-PLGA/GO hybrid fiber matrices supported the cellular response in terms of cell proliferation and osteogenesis differentiation in the presence of electrical stimulation, which could be a potential treatment for bone defect.

Herbally Painted Biofunctional Scaffolds with Improved Osteoinductivity for Bone Tissue Engineering

2019 ◽  
Vol 41 ◽  
pp. 49-68 ◽  
Author(s):  
Shivaji Kashte ◽  
Gajanan Arbade ◽  
R.K. Sharma ◽  
Sachin Kadam
Keyword(s):  
Tissue Engineering ◽  
Cell Proliferation ◽  
Tensile Strength ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Cell Attachment ◽  
Biological Properties ◽  
Osteogenic Potential ◽  
Composite Scaffolds ◽  
Alizarin Red

In the bone tissue engineering composite scaffolds with osteogenic potential are emerging as the new tool. Here, we investigated the graphene (GP), graphene oxide (GO) andCissusquadrangularis(CQ) callus extract for their spontaneous osteoinductive potential. Electrospun poly ε-caprolactone (PCL) sheets were painted with varying combination GP, GO and CQ solutions as ink. The prepared PCL-GO, PCL-GO-CQ, PCL-GP and PCL-GP-CQ scaffolds were characterized for their physical, mechanical and biological properties. Addition of GO, GP, GO-CQ and GP-CQ to PCL enhanced roughness, wettability, Yield strength and tensile strength, biocompatibility .significantly. Presence of GO and CQ in PCL-GO-CQ scaffolds, while GP and CQ in PCL-GP-CQ scaffolds showed synergistic effect on the biocompatibility, Cell attachment,cell proliferation of human umbilical Wharton’s jelly derived mesenchymal stem cells (hUCMSCs) and their differentiation into osteoblasts by 21stday in culture without osteogenic differentiation media or any growth factors. Same is confirmed by the Alizarin red S staining and Von kossa staining. The combination of PCL-GO-CQ scaffold prepared by novel paint method was found to be the most potential in bone tissue engineering.

Poly(lactic-co-glycolic)/Nanostructured Merwinite Porous Composites for Bone Tissue Engineering. I. Preparation and Morphology

2011 ◽  
Vol 493-494 ◽  
pp. 718-722 ◽  
Author(s):  
Masoud Hafezi-Ardakani ◽  
Faranak Kavian ◽  
Fatollah Moztarzadeh ◽  
Mohamadreza Baghaban Eslaminejad ◽  
Ali Zamanian ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Weight Loss ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Solvent Casting ◽  
Salt Leaching ◽  
Uniform Microstructure ◽  
Porous Composites ◽  
Phosphate Buffered Saline ◽  
Interconnected Pores

A novel merwinite/ Poly(lactic-co-glycolic) nanocomposite was synthesized by a solvent casting/salt leaching technique with varying merwinite contents from 10 to 30% (w/w). Poly(lactic-co-glycolic) /merwinite foams with a co-continuous structure of interconnected pores were formed. The microstructure of the pores and the walls was controlled by varying the merwinite content. The pore structure becomes more and more irregular with increasing merwinite content. Pore sizes ranging from several microns to a few hundred microns were obtained. The degradation assessment of the scaffolds is performed in phosphate-buffered saline (PBS) solution at 37°C. Weight loss during storage at 37°C in PBS (pH 7.4) was determined for the scaffolds. Weight loss increased from pure to high content during incubation time. The prepared merwinite/ (Polylactic-co-glycolic) nanocomposite with uniform microstructure may be used in bone tissue engineering applications.

Functionalization of Nanofibrous Spiral Structured Scaffolds for Bone Tissue Engineering

2009 ◽  
Vol 1235 ◽  
Author(s):  
Junping Wang ◽  
Xiaojun Yu
Keyword(s):  
Tissue Engineering ◽  
Cell Proliferation ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Spiral Structure ◽  
Nutrient Transport ◽  
Structure Design ◽  
Cellular Growth ◽  
Osteoblast Cells ◽  
Nanofibrous Scaffolds

AbstractIn the previous studies, we have successfully developed a novel spiral structured nanofibrous scaffolds with improved osteoconductivity for bone tissue engineering. The spiral structure design facilitates the nutrient transport and waste removal, and allows uniform cellular growth and distribution within the scaffolds, thus enhanced the bioactivity of the scaffolds. In this chapter, HAP and BMP-2 were incorporated within the nanofibrous spiral scaffolds in order to enhance the osteoinductivity of the established system. The effect of the blending materials was evaluated through cell proliferation, cell differentiation of human osteoblast cells seeded on the scaffolds and cultured for 4 and 8 days. The results has demonstrated that the functionalization of PCL nanofibrous spiral scaffolds leads to higher ALP expression level and increased amount of mineralization level however lower cell proliferation rate.

Porous Silk Fibroin/Alpha Tricalcium Phosphate Composite Scaffolds for Bone Tissue Engineering: A Preliminary Study

2016 ◽  
Vol 695 ◽  
pp. 164-169 ◽  
Author(s):  
Woradej Pichaiaukrit ◽  
Wiriya Juwattanasamran ◽  
Teerasak Damrongrungruang
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Pore Size ◽  
Bone Tissue ◽  
Silk Fibroin ◽  
Tricalcium Phosphate ◽  
Average Pore Size ◽  
Compressive Modulus ◽  
Tissue Engineering Application ◽  
Potential Candidate

Scaffolds with mechanical properties that mimic the tissue to be restored are critical to maintain the morphology and function of a scaffold after implantation and during tissue regeneration. Silk fibroin (SF), a protein from the Bombyxmori silk worm cocoon, is currently employed in the biomedical field and tissue engineering. The objective of this study was to construct three-dimensional porous silk fibroin/alpha tricalcium phosphate scaffolds for bone tissue engineering application. The scaffolds were fabricated using a solvent casting and salt leaching technique. The hybrid strain of degummed Thai silk fibroin, Nangnoi Srisaket 1 x Mor, was dissolved in hexafluoroisopropanol at 16% (w/v). Alpha tricalcium phosphate (α-TCP) was incorporated to produce 4, 8, 12, and 16 wt% solution and sucrose (particle size 250-450 μm; sucrose/silk fibroin = 8.5/1 w/w) was used as a porogen. The microstructure and pore size, calcium and phosphorus contents, and compressive modulus were evaluated. The scanning electron microscope images revealed the microstructure of scaffolds to be square shaped with continuous interconnected pores. The average pore size of the scaffolds was 265.70 + 67.45 μm. The scaffolds containing 8% (w/w) α-TCP exhibited the highest compressive modulus (64.84 + 16.65 kPa) and the highest calcium content. The results suggested that the scaffolds containing α-TCP may be a potential candidate for application in bone tissue engineering applications.

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