The Used of Protease from Palado (Agave) Roots, and Palado Leaf in the Making Process of Virgin Coconut Oil (VCO)

2019 ◽  
Vol 967 ◽  
pp. 123-131
Author(s):  
Sitti Rahmawati ◽  
Siti Nuryanti ◽  
KASMIR S. Male
Keyword(s):  
Enzyme Activity ◽  
Raw Materials ◽  
Coconut Oil ◽  
Acid Number ◽  
The Body ◽  
Virgin Coconut Oil ◽  
Mechanical Pressure ◽  
Traditional Medicines ◽  
Protease Enzyme ◽  
Enzymatic Methods

Virgin coconut oil (VCO) which is also known as coconut oil or virgin coconut is coconut oil obtained from fresh coconut meat through a process of heating at low temperatures, freeze drying, fermentation, enzymatic, mechanical pressure or centrifugation. VCO has a higher commercial value because it has many benefits and efficacy, which is only used for food and industrial raw materials for the cosmetic treatment of the body (hand body lotion), can also improve your metabolism and can cope with a variety of disease, thus used as ingredients in traditional medicines. One way of making the VCO is quite interesting by the enzymatic method using a protease derived from agave roots, and agave (palado) leaves. The purpose of this study was to examine the use of existing protease enzymes in plant roots palado, and agave leaves (leaf palado) as materials for VCO on enzymatic methods and and the determination of enzyme activity by Murachi method. The results showed that the enzyme that is at the root of the agave plant (palado root), and leaves of the agave plant (palado petals) can be used as an ingredient in the manufacture of VCO with enzymatic methods. VCO yield resulting from the material between palado leaves, and roots of palado, namely 17.5%. acid number of the resulting VCO is 0.56 (using a leaf Palado), who used the palado roots 0.44. The results of enzyme activity test show that protease enzyme contained in palado leaf extract is bromelin enzyme.

scholarly journals Potential Global Competitiveness of Sri Lankan Virgin Coconut Oil Industry

2018 ◽  
Vol 11 (1) ◽  
pp. 1520-1526
Author(s):  
Pivithuru Janak Kumarasinghe ◽  
Savinda Perera
Keyword(s):  
Sri Lanka ◽  
Market Share ◽  
Raw Materials ◽  
Coconut Oil ◽  
Oil Industry ◽  
Local Market ◽  
Sri Lankan ◽  
Virgin Coconut Oil ◽  
Export Competitiveness

The study focuses on Sri Lankan virgin coconut oil industry because of it is one of the upcoming export products and also its position as one of the key player in the global market. Sri Lankan coconut industry is one of the major foreign exchange and employment generation source and element of the Sri Lankan nation. The study attempted to unearth the determinants of export competitiveness of virgin coconut oil industry in Sri Lanka by drawing attention on Porter’s theory of the competitive advantage of nations. The target population of the study consisted with individual firms which are engaging in virgin coconut oil export in Sri Lanka is two hundred and nineteen. The study used a likert scale to measure the chosen variables. Based on the Pearson Correlation analysis researcher can say that there is significance strong positive relationship between Availability of Raw materials, Quality of demand and Market share of export with the Export Competitiveness. According to regression analysis researcher can say that availability of Raw materials, Local market, Quality of demand and Market share of export has significance positive affect on Advantage of Export Competitiveness.

scholarly journals REVIEW: PEMANFAATAN BROMELAIN PADA BEBERAPA PANGAN LOKAL INDONESIA

2019 ◽  
Vol 10 (1) ◽  
pp. 33
Author(s):  
Warsono El Kiyat ◽  
Kevin Reynaldo ◽  
Jeremiah Irwan ◽  
Eryd Saputra
Keyword(s):  
Local Food ◽  
Ph Value ◽  
Raw Materials ◽  
Coconut Oil ◽  
Water Soluble ◽  
Ananas Comosus ◽  
Soluble Nitrogen ◽  
Fish Sauce ◽  
Virgin Coconut Oil

Bromelain is one of the protease that can be produced from all of parts of the pineapple plants (Ananas comosus). It has potential to improve quality of local Indonesian food such as tempeh gembus, virgin coconut oil (VCO), and dumbo catfish based fish sauce (DCBFS). Tempeh gembus is a traditional food that has unique characteristics especially in its taste because it contains amino acids. VCO attracts consumers because of its health aspect. Its raw materials are available in Indonesia. DCBS  is a product of fermented fish with salt that has a distinctive taste and is produced in Indonesia. This study aimed to analyze the effect of bromelain on local Indonesian food and its applications. The result showed that the use of bromelain in local Indonesian food could improve the quality and nutritional value of local food. Bromelain can be used in local food like tempeh gembus, virgin coconut oil, and fish sauce from dumbo catfish. The use of bromelain in tempeh gembus could  increase both of the water-soluble nitrogen level and the pH value. However, the addition of bromelain in the production of virgin coconut oil and fish sauce from dumbo catfish could increase the yield of theirs. .

scholarly journals PROSES PEMBUATAN BIODIESEL DARI CAMPURAN MINYAK KELAPA DAN MINYAK JELANTAH

Konversi ◽  
2018 ◽  
Vol 5 (1) ◽  
pp. 8
Author(s):  
Muthia Elma ◽  
Satria Anugerah Suhendra ◽  
Wahyuddin Wahyuddin
Keyword(s):  
Water Content ◽  
Palm Oil ◽  
Raw Materials ◽  
Coconut Oil ◽  
Waste Cooking Oil ◽  
Acid Number ◽  
Esterification Reaction ◽  
Cooking Oil ◽  
Ester Content ◽  
Total Glycerol

Abstrak-Indonesia memiliki hasil produksi buah kelapa yang hanya dimanfaatkan untuk memasak. Minyak jelantah merupakan hasil dari sisa penggorengan rumah tangga yang setelah penggunaanya menjadi limbah dan dapat mencemari lingkungan. Penelitian ini bertujuan untuk memproduksi biodiesel dengan memanfaatkan campuran antara minyak kelapa dan minyak jelantah terhadap efek penambahan metanol dan waktu reaksi optimum dari pembuatan biodiesel. Proses produksi biodiesel dari campuran kedua bahan baku menggunakan proses dimana minyak kelapa dan minyak jelantah dicampurkan berdasarkan %-v/v dari 200 mL dengan perbandingan minyak jelantah (MJ) dan minyak kelapa (MK) yaitu 100MJ:0MK; 75MJ:25MK; 50MJ:50MK; 25MJ:75MK; dan 0MJ:100MK dengan komposisi metanol serta esterifikasi 38%; 30%; 28%; 19% serta untuk trasesterifikasi 19%; 20%; 21%; 25%. Pada reaksi esterifikasi menggunakan komposisi katalis H2SO4 0,5%, dan transesterifikasi menggunakan katalis KOH 0,9%. Yield yang dihasilkan dari penelitian ini adalah: 100MJ:0MK 92,15%; 93,65%, 75MJ:25MK (96,65%), 50MJ:50MK (95,11%), 25MJ:25MK (96,65%) dan 100MK:0MJ (82,65%). Analisa gliserol total yang didapat pada penelitian ini adalah 100MJ:0M (0,19%), 75MJ:25MK (0,21%), 50MJ:50MK (0,23%) 25MJ:25MK (0,22%) dan 100MK:0MJ (0,26%). Dari hasil analisa gliserol total tersebut didapat sampel yang terbaik yakni 50MJ:50MK dengan nilai glirserol total 0,23% dengan waktu 60 menit untuk esterifikasi dan 70 menit untuk transesterifikasi, dengan analisa angka asam yang didapatkan sebesar 0,2117, angka penyabunan 198,41; ester content  yang didapat sebesar 98,163% water content untuk sebesar 0,56 ppm. Keseluruhan dari hasil analisa biodiesel tersebut memenuhi standar EN 14214.  Kata kunci: minyak kelapa, minyak jelantah, biodiesel, FFA, trigliserida, gliserol total.  Abstract-Coconut oil is normally produced as cooking oil in some areas in Indonesia. However, palm oil mostly produced by industries as vegetable/cooking oil.Waste cooking oil from palm oil becomes a big problem in the environment, and creates pollution. This research aims to use waste cooking oil to produce biodiesel by mixing waste cooking oil and coconut oil. Those mixed oils become raw materials for this proces. The composition of the mixtures are  100MJ: 0MK; 75MJ: 25MK; 50MJ: 50MK; 25MJ: 75MK; and 0MJ: 100MK (% v / v of waste cooking oil (MJ) and coconut oil (MK)).The total of 200 mL oil mixtures was used for the esterification process with methanol composition were 38%; 30%; 28%; and trans-esterification were 19%; 20%; 21%; 23%. Esterification reaction was using the 0,5% H2SO4 as a catalyst, while transesterification was using 0.9% KOH as catalyst. The yield of biodiesel this reaserch were: 100MJ: 0MK (92.15%), 75MJ: 25MK (96.65%), 50MJ: 50MK (95.11%), 25MJ: 25MK (96.65%) and 100MK: 0MJ (82.65%). Furthermore, the total glycerol values were 100MJ:0MK (0.19%), 75MJ: 25MK (0.21%), 50MJ:50MK (0.23%) 25MJ: 25MK (0.22%) and 100MK: 0MJ (0.26%). EN14214 standard shows that the best composition of mixtured oils was 50MJ:50MK. Then, the total glycerol was 0.23% (60-70 minutes for the esterification and transesterification reaction). Acid number value was 0.2117, saponification number was 198.41; ester content was 98.163% and water content was 0.56 ppm.  Keywords: coconut oil, waste cooking oil, biodiesel, FFA, triglyceride, total glycerol.

scholarly journals ANALISIS KELAYAKAN AGROINDUSTRI VIRGIN COCONUT OIL(STUDI KASUS KELOMPOK TANI ANUGRAH KELURAHAN TANDURUSA KECAMATAN AERTEMBAGA KOTA BITUNG)

2016 ◽  
Vol 12 (3A) ◽  
pp. 147
Author(s):  
Sakila ., Rahman ◽  
Vicky V.J. Palenewen ◽  
Femi H. Elly
Keyword(s):  
Raw Materials ◽  
Coconut Oil ◽  
Variable Cost ◽  
Fixed Costs ◽  
Total Revenue ◽  
Sample Collection ◽  
Virgin Coconut Oil ◽  
Group Data ◽  
Break Even Point ◽  
Result Analysis

This study aims to analyze how much profit out of the business of agro-industry Virgin Coconut Oil and analyzing the feasibility of agroindustrial Virgin Coconut Oil of Grace Farmers Group in Tandurusa Village Aertembaga Sub=District, District of Bitung. This research method is a method of Agro-industry survey on business Tandurusa Grace Farmer Group. Data were collected through interviews using a questionnaire technique. Methods of sample collection is done use purposive sampling) are members of farmer groups Grace. Analyzed using profits to the equation TC = TFC + TVC, TR = Q. P, Π = TR - TC. Then proceed to calculate the value of R / C which is the ratio between the total revenue with total costs incurred during the production process, wherein if R / C> 1 = worthy, R / C = 1 = breakeven, R / C <1 = not feasible. To find out if this Virgin Coconut Oil Agroindustri no profit or loss by the use of analysis Break Even Point (BEP). Result analysis and discussion shows that the total variable cost is Rp. 81.96 million, / year and total fixed costs are Rp.8.295.000, - / year, while total revenue was Rp. 249 600 000, - and BEP production was 61.75 liters and the BEP price is Rp.12.343.750 / year and Rp. 1,028.645, - / month and Rp. 85.720, - / 2day. Total profit / year is Rp. 159 345 000, - / year. With the value of R / C ratio is 3, so that it can be concluded that the Virgin Coconut Oil Agroindustri Poktan Grace Village Tandurusa experiencing gains and financially feasible to be developed because it exceeds the BEP and also R / C Ratio of her that is> 1. Fluctuations in the price and availability of raw materials greatly influence Virgin Coconut Oil production. Venture capital also influences the production of Virgin Coconut Oil. Agribusiness Virgin Coconut Oil provides an opportunity to increase income of farmers' income and employment opportunities and improve the State Foreign Exchange.

Pengaruh Pemberian Virgin Coconut Oil Terhadap Kadar Kreatinin dan Urea Nitrogen Darah Rattus Norvegicus Jantan

2020 ◽  
Vol 17 (2) ◽  
pp. 181
Author(s):  
NOVIANE ANGRELLA ◽  
ROOSTANTIA INDRAWATI ◽  
LESTARI DEWI
Keyword(s):  
Creatinine Level ◽  
Blood Urea Nitrogen ◽  
Coconut Oil ◽  
The Body ◽  
Virgin Coconut Oil ◽  
Urea Nitrogen ◽  
Waste Products ◽  
Nitrogen Levels ◽  
Functional Product ◽  
Male Wistar Rats

<p><strong>ABSTRACT</strong></p><p><strong>Background:</strong><em> </em><em>Virgin Coconut Oil </em>(VCO) is a functional product that is highly favored and have been widely applied in the everyday lives of society. The increase of the frequencies and duration of VCO consumption is suspected that VCO could negatively impact the target organ. The kidney is a vital organ in the human body, it is responsible for clearing out the residual waste products of metabolism in the body. The evalution of the kidney’s function can be done through the measurement of creatinine and blood urea nitrogen. The purpose of this study is to determine the effect of VCO administration on kidney function.</p><p><strong>Method</strong>: 24 male wistar rats that are divided into 4 groups, which are listed as follows, K(-) or a negative-controlled group, P(1) or the first group of rats that are given VCO with a dose of 2mL/200gr of the rats’ total BW/day, P(2) or the second group of rats that are given VCO with a dose of 3mL/200gr of the rats’ total BW/day,  and P(3) or the third group of rats that are given VCO with a dose of 4mL/200gr of the rats’ total BW/day for 28 day</p><p><strong>Results</strong>: Administrating<strong> </strong>VCO with a dose of 2mL/200gr and 3mL/200gr of the rats’ total BW/day does not affect the blood’s creatinine level while with a dose of 4mL/200gr of the rats’ total BW/day decreases the blood’s creatinine level. Administrating VCO with a dose of 2 mL/200gr, 3 mL/200gr, and 4 mL/200gr of the rats’ total BW/day does not affect the level of blood urea nitrogen.</p><p><strong>Conclusion: </strong>Administration of VCO dose of 4 mL / 200 g BW rat / day significantly decreased blood creatinine levels in experimental animals, while other doses did not affect the creatinine levels or blood urea nitrogen levels<strong></strong></p><p><strong>Keywords</strong><strong>:</strong> <em>Virgin Coconout Oil </em>(VCO), cretinine, blood urea nitrogen.</p>

scholarly journals Data on the use of virgin coconut oil and bioethanol produced from sugar palm sap as raw materials for biodiesel synthesis

Data in Brief ◽  
2020 ◽  
Vol 29 ◽  
pp. 105199
Author(s):  
Sanusi Gugule ◽  
Feti Fatimah ◽  
Chaleb Paul Maanari ◽  
Trina Ekawati Tallei
Keyword(s):  
Raw Materials ◽  
Coconut Oil ◽  
Virgin Coconut Oil ◽  
Biodiesel Synthesis

scholarly journals The Effect of Giving Virgin Coconut Oil (VCO) to Changes in Lauric Acid Levels in Breast Milk

2021 ◽  
Vol 5 (2) ◽  
pp. 233-243
Author(s):  
Ismul Sadly Putra ◽  
Joserizal Serudji
Keyword(s):  
Fatty Acids ◽  
Breast Milk ◽  
Lauric Acid ◽  
Metabolic Pathways ◽  
Saturated Fatty Acids ◽  
Coconut Oil ◽  
The Body ◽  
Virgin Coconut Oil ◽  
Nutritional Components ◽  
Anti Fungal

Breastmilk (ASI) is the best food produced by the breast glands, containing all the nutritional elements needed by infants aged 0-6 months. Apart from nutrients, breast milk also has other non-nutritional components that act as immune factors for babies. Babies who are not exclusively breastfed will be more susceptible to chronic diseases, such as heart disease, hypertension, and diabetes as adults and can suffer from malnutrition and obesity. Dietary intake significantly affects milk production. The mother's diet can affect the composition of her milk through several metabolic pathways, especially the concentration of fatty acids and vitamins that are soluble in fat and water, including vitamins A, C, B6, and B12. Lauric acid contained in breast milk can help your minor fight infection and increase immunity to protect from various diseases. Not only for the baby, but virgin coconut oil is also starting to be commonly used by dieters. Virgin coconut oil contains lauric acid CH3 (CH2) 10COOH 50% and caprylic acid CH3 (CH2) 6COOH 7%. Both of these acids are medium-chain saturated fatty acids that are quickly metabolized and have anti-microbial properties. The benefits of virgin coconut oil (VCO) are that it is beneficial for the health of the body, including being antibacterial, antiviral, anti-fungal, and natural anti-protozoa; helps relieve symptoms and reduces health risks associated with diabetes, helps protect against osteoporosis, helps prevent high blood pressure, helps prevent liver disease, keeps the heart and blood vessels healthy, helps prevent cancer, helps you lose weight, maintain stamina, maintain healthy skin and hair.Keywords: Breastmilk, Lauric Acid, VCO

scholarly journals Pemurnian Minyak Kelapa Berbahan Baku Kopra / Refining of Coconut Oil Made of Copra

Buletin Palma ◽  
2017 ◽  
Vol 17 (2) ◽  
pp. 155
Author(s):  
Fahri Ferdinan Polii
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Raw Materials ◽  
Coconut Oil ◽  
Research Process ◽  
Acid Number ◽  
Active Charcoal ◽  
Physical Parameters ◽  
Cooking Oil

<p>Processing of copra is generally done by drying through direct or fogging with warming temperatures. This way produces coconut oil with low quality rough, because the content of water and free fatty acids high, so quickly turn rancid, brownish and unfit in the consumption. The purpose of this research process of purification yaitumelakukan coconut oil made of copra smog became a good-quality cooking oil. Research use descriptive method. Research carried out the month on June 2015 until January 2015 in Research and Standardization of Industrial Institute in Manado, North Sulawesi. The raw materials used in the study of copra comes from Bengkol Village in Manado. Processed into copra oil and refined oil. The results of the analysis of the quality of coconut oil before refining the parameters color, odor, moisture content, free fatty acids and acid number does not qualify SNI. After the process of purification by neutralization using NaOH 18 oBe and 20 oBe and performed an analysis of the quality of the oil, it turns out that the use of NaOH 20 oBe effective improve quality coconut oil good physics and chemistry. Coconut oil results neutralization with 20 NaOH oBe continued with the process of bleaching  using active charcoal 2% and mix active charcoal  1% + 1% bentonite improved the quality of the oil either chemical or physical parameters and qualify SNI cooking oil. Yield after cooking oil with  neutralised NaOH 20oBe and bleaching using active charcoal 2% i.e. 78,79%.</p><p align="center"><strong>ABSTRAK</strong></p><p>Pengolahan kopra pada umumnya dilakukan  dengan cara pengeringan melalui  pemanasan langsung atau pengasapan dengan suhu yang tinggi. Cara ini menghasilkan minyak kelapa kasar mutu rendah, karena kandungan air dan asam lemak bebasnya tinggi, sehingga cepat menjadi tengik, warna kecoklatan dan tidak layak di konsumsi. Tujuan penelitian ini yaitu melakukan proses pemurnian  minyak kelapa berbahan baku kopra asap  menjadi minyak goreng yang bermutu baik. Penelitian menggunakan metode deskriptif. Penelitian dilakukan bulan pada Pebruari 2015 sampai dengan November 2015 di Balai Riset dan Standardisasi Industri Manado, Sulawesi Utara. Bahan baku kopra  yang digunakan dalam penelitian ini berasal dari Kelurahan  Bengkol Manado. Kopra diproses menjadi minyak dan minyak dimurnikan. Hasil analisis mutu minyak kelapa sebelum pemurnian  untuk parameter bau, warna, kadar air, asam lemak bebas dan bilangan asam tidak memenuhi syarat SNI. Proses pemurnian  dengan netralisasi menggunakan larutan NaOH 18 oBe dan 20 oBe,  ternyata larutan NaOH 20 oBe efektif meningkatkan mutu minyak kelapa  dan  memenuhi syarat mutu SNI. Minyak kelapa hasil netralisasi dengan NaOH 20 oBe dilanjutkan dengan proses pemucatan  menggunakan arang aktif 2% dan campuran arang aktif 1%+bentonit 1% terjadi peningkatan mutu minyak baik parameter fisika maupun  kimia dan memenuhi syarat SNI minyak goreng.  Rendemen minyak goreng setelah dinetralisasi dengan larutan  NaOH 20 oBe dan  pemucatan menggunakan arang aktif 2%, yakni 78,79%.</p>

scholarly journals Characteristics of Physical, Chemical, and Organoleptic Properties of Virgin Coconut Oil (VCO) By Studying the Ratio Between Coconut Cream with Inducement Oil and Length of Fermentation

2020 ◽  
Vol 68 (3) ◽  
pp. 473-482
Author(s):  
Chairil Anwar ◽  
Irmayanti ◽  
Umar HA
Keyword(s):  
Coconut Oil ◽  
Acid Number ◽  
Raw Material ◽  
Virgin Coconut Oil ◽  
Physical Chemical ◽  
Two Factors ◽  
Increase Productivity ◽  
Effect On Yield ◽  
Processed Products ◽  
Coconut Cream

Coconut agribusiness development plays an important role in an effort to increase productivity as a raw material for processed products from coconut. Indonesia is one of the world's largest coconut plantation. Unfortunately, Indonesia's exports are in the form of ordinary coconut oil, while Philippines has begun to reach the world with the Virgin Coconut Oil (VCO). However, there is still a lack of diversification that can be done by coconut farmers made its little progress. In fact, the price of VCO which tripled from coconut oil used to make the oil can be potential to be developed in Indonesia The purpose of this research was to examine the cream ratio with inducement oil and length of fermentation on VCO quality. The research used a factorial randomized complete design (CRD) of 3 × 3 consisting of two factors, namely the ratio of coconut cream and inducement oil (A) and length of fermentation (B). The ratio of coconut cream and inducement oil (A) consisted of three levels, namely: A1 = 2 : 1, A2 = 3 : 1, and A3 = 4 : 1. Length of fermentation factors (B) consisted of three levels, namely: B1 = 8 hours, B2 = 10 hours, and B3 = 12 hours. The results showed that the treatment of cream ratio with inducement oil had a very significant effect on the yield, organoleptic aroma, and color. The length of fermentation had a very significant effect on yield, organoleptic aroma and color. The interaction between the two factors had a very significant effect on all parameters (water content, peroxide number, acid number, and iodine number) except yield and organoleptic (aroma and color) which had no effect. The best VCO quality was obtained by ratio of cream with inducement oil 2 : 1 and 8 hours length of fermentation.

scholarly journals Efektifitas Penggunaan Virgin Coconut Oil (VCO) Terhadap Pencegahan Diaper Rash pada Bayi Usia 1-12 Bulan di Wilayah Kerja Puskesmas Depok II Sleman

2014 ◽  
Vol 1 (2) ◽  
pp. 85
Author(s):  
Jennifa Jennifa ◽  
Atik Ba'diah ◽  
Eni Purwaningsih
Keyword(s):  
Data Analysis ◽  
Coconut Oil ◽  
The Body ◽  
P Value ◽  
Univariable Analysis ◽  
Virgin Coconut Oil ◽  
Medium Chain Triglycerides ◽  
Chi Square ◽  
Bivariable Analysis ◽  
Chi Square Test

Background : Diaper rash is a dermatitis eclusively localized in the diaper area, commonly attack children less than 2 years of age, usually conservatively cured by the parents so that only 7-35% babies suffer from diaper rash. Virgin Coconut Oil (VCO) that are beneficial to health, has been widely sought after to resolve health problems, also acts against bacteria and microbe. The Content of Medium Chain Triglycerides (MCT) in VCO hydrolyzed faster, and more quickly absorbed in the body tissue. Aim :The aim of this research is to determine the effect of Virgin Coconut Oil on prevention diaper rash. Methods : This research is pre-experimental with posttest only design.  Conducted from May to June 2014. The research was done in Puskesmas Depok II Sleman area, with 14 respondents according to the determined criteria. Respondents were instructed to apply VCO in the diaper area once every morning after bath  for 30 days. Data analysis used univariable analysis, bivariable analysis with Chi-Square test. Result :The results of the data obtained in this study was 78.6% of respondents regularly use VCO, but not experiencing diaper rash and there are 21.4% of respondents do not regularly use VCO and 14.3% had diaper rash. The results showed there was significant effect of VCO on prevention diaper rash with a significance p value = 0,003(p<0,05). Conclusion : Therefore it can be concluded that applying of VCO has a significant effective on prevention diaper rash. The use of VCO one time in the morning after a shower made ​​for ≥21 days was more effective against the prevention of diaper rash compared with the use of VCO <21 days.

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