scholarly journals Effect of mastitis on macro-minerals of bovine milk and blood serum in Sudan

Author(s):  
I.E.M. El Zubeir ◽  
O.A.O. ElOwni ◽  
G.E. Mohamed

Milk and blood serum from clinically mastitis infected, subclinically mastitis infected and healthy Friesian cows (15 samples from each of 3 groups) were evaluated for macrominerals (sodium, potassium, calcium, magnesium and phosphorus). The milk from cows infected with subclinical mastitis revealed a significant decrease in potassium (P < 0.001) and a significant increase in sodium and phosphorus content (P < 0.01). Similarly, the milk from cows with the clinical form of the disease showed a significant increase in sodium (P < 0.001) and a significant decrease in potassium, magnesium (P < 0.001) and calcium (P < 0.01). Comparison of healthy cow's milk with that from cows with subclinical mastitis revealed a highly significant increase in sodium (P < 0.001). Comparison of healthy cow's milk with that of clinically mastitic milk showed a highly significant decrease in levels of calcium, magnesium (P < 0.001) and potassium (P < 0.01). However, sodium increased highly significantly (P < 0.001). Comparison of macro-minerals in milk from cows with subclinical and clinical mastitis revealed a significant decrease in potassium contents (P < 0.05) compared with that of healthy cows. Potassium levels were found to decrease significantly (P < 0.05) in subclinically infected cow's blood serum. However, calcium and phosphorus showed a significant decrease (P < 0.01) in blood serum samples from the clinically infected cows.

1981 ◽  
Vol 48 (3) ◽  
pp. 387-392 ◽  
Author(s):  
Masaharu Oshima ◽  
Hiroshi Fuse

SummaryThe effect of subclinical mastitis on the citric acid concentration of cow's milk was studied by comparing milk from the mastitic quarter with that from a healthy quarter of the same udder. Forty-two pairs of fore-milk from the morning milking of 17 cows were examined and the citric acid content was measured by an automated carboxylic acid analyzer. The citric acid concentration decreased in subclinical mastitic milk and the extent of decrease was proportional to the degree of mastitis as expressed by the quarter difference value in concentration of Na+Cl in mm/1, i.e. the increase in the Na and Cl concentrations in the abnormal milk over that found in normal milk from the same udder. The correlation coefficient between the quarter difference value of Na+Cl and the value for citric acid was –0·77, (d.f. = 46, P <0·001) and a linear regression equation was obtained. The result showed clearly that subclinical mastitis is one of the causes of variation in the citric acid content of cow's milk. The quantitative change in citric acid concentration in subclinical mastitic milk is discussed with reference to the admixture theory for the formation of abnormal milk.


Dairy ◽  
2021 ◽  
Vol 2 (2) ◽  
pp. 191-201
Author(s):  
Young W. Park ◽  
George F. W. Haenlein

A new type of cow’s milk, called A2 milk, has appeared in the dairy aisles of supermarkets in recent years. Cows’ milk generally contains two major types of beta-casein as A1 and A2 types, although there are 13 genetic variants of β-casein: A1, A2, A3, A4, B, C, D, E, F, H1, H2, I and G. Studies have shown that A1 β-casein may be harmful, and A2 β-casein is a safer choice for human health especially in infant nutrition and health. The A2 cow milk is reportedly easier to digest and better absorb than A1 or other types of milk. The structure of A2 cow’s milk protein is more comparable to human breast milk, as well as milk from goats, sheep and buffalo. Digestion of A1 type milk produces a peptide called β-casomorphin-7 (BCM-7), which is implicated with adverse gastrointestinal effects on milk consumption. In addition, bovine milk contains predominantly αs1-casein and low levels or even absent in αs2-casein, whereby caprine milk has been recommended as an ideal substitute for patients suffering from allergies against cow milk protein or other food sources. Since goat milk contains relatively low levels of αs1-casein or negligible its content, and αs2-casein levels are high in the milk of most dairy goat breeds, it is logical to assume that children with a high milk sensitivity to αs1-casein should tolerate goat milk well. Cow milk protein allergy (CMPA) is considered a common milk digestive and metabolic disorder or allergic disease with various levels of prevalence from 2.5% in children during the first 3 years of life to 12–30% in infants less than 3 months old, and it can go up to even as high as 20% in some countries. CMPA is an IgE-mediated allergy where the body starts to produce IgE antibodies against certain protein (allergens) such as A1 milk and αs1-casein in bovine milk. Studies have shown that ingestion of β-casein A1 milk can cause ischemic heart disease, type-1 diabetes, arteriosclerosis, sudden infant death syndrome, autism, schizophrenia, etc. The knowledge of bovine A2 milk and caprine αs2-casein has been utilized to rescue CMPA patients and other potential disease problems. This knowledge has been genetically applied to milk production in cows or goats or even whole herds of the two species. This practice has happened in California and Ohio, as well as in New Zealand, where this A2 cow milk has been now advanced commercially. In the USA, there have been even promotions of bulls, whose daughters have been tested homozygous for the A2 β-casein protein.


Author(s):  
Mourad HAMIROUNE ◽  
Sounia DAHMANI ◽  
Zineb KASMI ◽  
Abdelhamid FOUGHALIA ◽  
Mahmoud DJEMAL

This research was conducted to study the key physicochemical parameters of samples of raw bovine and goat milk collected in the steppic region of Djelfa. One hundred and six samples of raw milk were collected from April 2018 to May 2018, at points of sale and analyzed. The results showed that cow’s milk had 3.66±0.89% fat, 11.4±1.56% solid not fat, 4.35±0.61% protein, 6.35±0.89% lactose and a density of 1.0360±0.0056 with a freezing point of -0.380±0.053 °C. While goat’s milk had 3.43±0.65% fat, 10.2±0.92% solid not fat, 3.88±0.36% protein, 5.66±0.52% lactose and a density of 1.0317±0.0035 with a freezing point of -0.348±0.044 °C. This proves that cow’s milk has a slightly higher physicochemical quality than goat’s milk. In addition, the present study showed that 100% raw goat milk is wet against 97.1% raw bovine milk. This indicates the presence of cases of fraud requiring disciplinary procedures. Moreover, in the majority of the cases, the storage temperatures of the milk far exceed the values recommended by the Algerian standards (+6°C). It is necessary to establish a program of control and popularization of all the actors of the sector in order to improve the quality and the quantity of raw milk produced.


2019 ◽  
Vol 63 (1) ◽  
pp. 93-97 ◽  
Author(s):  
Hanna Różańska ◽  
Aleksandra Lewtak-Piłat ◽  
Maria Kubajka ◽  
Marcin Weiner

Abstract Introduction: The aim of the study was to evaluate the occurrence of enterococci in inflammatory secretions from mastitic bovine udders and to assess their antimicrobial resistance. Material and Methods: A total of 2,000 mastitic milk samples from cows were tested in 2014–2017. The isolation of enterococci was performed by precultivation in buffered peptone water, selective multiplication in a broth with sodium azide and cristal violet, and cultivation on Slanetz and Bartley agar. The identification of enterococci was carried out using Api rapid ID 32 strep kits. The antimicrobial susceptibility was evaluated using the MIC technique. Results: Enterococci were isolated from 426 samples (21.3%). Enterococcus faecalis was the predominant species (360 strains), followed by E. faecium (35 isolates), and small numbers of others. The highest level of resistance was observed to lincomycin, tetracycline, quinupristin/dalfopristin (Synercid), erythromycin, kanamycin, streptomycin, chloramphenicol, and tylosin. Single strains were resistant to vancomycin and ciprofloxacin. All isolates were sensitive to daptomycin. E. faecalis presented a higher level of resistance in comparison to E. faecium, except to nitrofurantoin. Conclusion: The results showed frequent occurrence of enterococci in mastitic cow’s milk and confirmed the high rate of their antimicrobial resistance.


2000 ◽  
Vol 25 ◽  
pp. 165-168
Author(s):  
B.A. Slaghuis ◽  
G.H. Klungel

AbstractThe freezing point of bovine milk is known to vary between narrow limits. However, some variation is possible, because of the osmotic relationship between blood and milk. The extent of variation in freezing points of cow's milk was studied. For one year, freezing points were determined in individual milk samples from a high producing herd. Differences (P<0.05) were found between evening and morning milk yield and freezing points. A ‘lactation curve’ for freezing points was fitted and showed some similarity with milk yield curves. Stage of lactation explained part of the variation of freezing points of cow's milk.


Author(s):  
Marini Damanik ◽  
Hafni Indriati Nasution ◽  
Rini Selly ◽  
Moondra Zubir

Coconut milk is a thick white liquid extracted from coconut produced from shredded coconut and then squeezed with water. Coconut milk is an ingredient produced from processed old coconut. Coconut milk can be used as food preparations or as herbal beauty ingredients. One of the advantages of coconut milk in the field of beauty is that it is very effective as a dandruff remover. By using old coconut milk as a dandruff remover we can get amazing results with natural ingredients without spending a lot of money. Coconut milk has many contents such as water content, protein and fat which are quite high as well as cow's milk. Coconut milk is rich in vitamins C, B, B-6, thiamine, niacin, folate, a number of minerals (calcium, magnesium, iron and phosphorus). Pure coconut milk only contains 54 percent water, 35 percent fat and 11 percent nonfat solids (approximately 6 percent carbohydrates, less than 4 percent protein and other solids) which are categorized as emulsions of oil in water. Coconut milk is known to be effective in eliminating dandruff, because coconut milk contains coconut oil containing Vitramin E and fatty acids that can eliminate and eradicate dandruff. You do this by using coconut oil as a massage oil to massage the scalp.


1933 ◽  
Vol 4 (2) ◽  
pp. 279-284 ◽  
Author(s):  
Geoffrey Lewis Peskett ◽  
Sydney John Folley

1. Traces of blood serum albumin have been demonstrated in normal milk.2. The content of blood serum albumin is increased in many of the milks which are poor in non-fatty solids and there is evidence of some correlation between low non-fatty solids and high blood serum albumin content of the milk.3. The experimental data support a conclusion that deficiency of nonfatty solids in milk in some cases may be due to dilution of a normal secretion in the udder by a fluid of the nature of oedema fluid, i.e. to a pathological rather than a physiological condition.4. Some evidence, which obviously needs confirmation, has been adduced indicating that the casein-nitrogen content of milk as determined by Moir's technique may be slightly too high. The accuracy of the globulin figures seems also to be open to doubt.


2021 ◽  
Vol 5 (1) ◽  
pp. 17-20
Author(s):  
E.A. Vasilyeva ◽  
◽  
Yu.E. Rusak ◽  
E.N. Efanova ◽  
L.N. Lebedeva ◽  
...  

Aim: to study the association of food and inhalation sensitization with the severity of atopic dermatitis (AtD) in order to improve its treatment and prevention methods. Patients and Methods: the study included 50 patients with AtD from 2 to 44 years old. The study included clinical and laboratory research methods. The survey analyzed the history and anamnesis vitae, estimated the age at the AtD onset, determined the nature of concomitant pathology, the possible causes of cutaneous exacerbation, the allergic severity in the family history, the severity score on the SCORAD (Scoring of Atopic Dermatitis) scale and the efficacy of previous therapy. The immunological examination consisted of the quantitative determination of specific IgE and IgG antibodies in the blood serum to inhaled and food allergens by immunochemiluminescence assay on an IMMULITE 2000 XPi analyzer. Results: according to the detection frequency of IgE to food allergens in patients with AtD: the leading positions were taken by the chicken egg (egg white in 26% of cases, egg yolk in 20%); IgE to banana was detected in 17% of cases; in 12% — to cow’s milk, more common — to the β-lactoglobulin fraction. When studying the activity of inhaled allergens, respiratory sensitization to the cat epithelium was detected in 26% of cases, and to black birch — in 24%. Hypersensitivity to herbs (wormwood, herbal mixture) and house dust allergens (8%, respectively) was found much less common. In severe AtD, IgE to respiratory allergens prevailed: at n=38, the IgE concentration to cat epithelium and black birch was 12.4±1.4 IU/mL each. Besides, there was a significant sensitization to food allergens, namely, the IgE concentration to egg white — 5.4±0.9 IU/mL. Conclusion: the study revealed the sensitization of patients with AtD to food (mainly egg white, egg yolk, banana and cow’s milk) and respiratory (mainly cat epithelium, black birch) allergens. Determination of the IgE concentration in the blood serum of patients showed that there was a significant concentration increase to egg white, cat and black birch in severe AtD. Therefore, it is advisable to test for specific allergens in all patients with moderate to severe AtD. KEYWORDS: food sensitization, inhalation sensitization, atopic dermatitis, allergy, allergens, antibodies, immunochemiluminescence assay, IgE, triggers. FOR CITATION: Vasilyeva E.A., Rusak Yu.E., Efanova E.N., Lebedeva L.N. Food and inhalation sensitization in atopic dermatitis. Russian Medical Inquiry. 2021;5(1):17–20. DOI: 10.32364/2587-6821-2021-5-1-17-20.


2021 ◽  
Author(s):  
Nathália C.C. Silva ◽  
Marjory X. Rodrigues ◽  
Ana C.C.H. Tomazi ◽  
T. Tomazi ◽  
Bruna L. Crippa ◽  
...  

Abstract Background Staphylococcus spp. are among the most isolated bacteria from cases of clinical and subclinical mastitis in dairy cattle. Also, Staphylococcus is commonly isolated from bovine mammary secretions, not exclusively from intramammary infections. The genus comprises bacteria capable to form biofilm, produce toxins and acquire multi-drug resistance. The aims of this work were to evaluate the genetic virulence and antimicrobial resistance features of Staphylococcus spp. isolated from bovine milk using whole genome sequencing, and build a phylogenetic tree with gene sequences of Staphylococcus spp. isolated from clinical mastitis and non-clinical fresh cows. Results The bacterial collection comprised 29 Staphylococcus strains isolated from clinical mastitis cases (n = 7) and milk samples collected from fresh cows (n = 22). Strains were identified as Staphylococcus aureus (n = 2), Staphylococcus chromogenes (n = 19), and Staphylococcus haemolyticus (n = 8). After sequences quality control, twenty-three strains had good quality of contigs to be included in the further analysis. Conclusions Ninety-four virulence genes were observed, including pvl, icaA, icaD and MMSCRAMS genes. We also detected important resistance genes as blaZ, ant(4), erm(B), fexA, lnu(D), tet(L) and tet(M). The phylogenetic tree related the species as expected and presented four clades.


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