Activity of intestinal mucosal membrane carbohydrases in cattle of different breeds

1997 ◽  
Vol 77 (3) ◽  
pp. 441-446 ◽  
Author(s):  
P. S. Mir ◽  
D. R. C. Bailey ◽  
Z. Mir ◽  
S. D. Morgan Jones ◽  
H. Douwes ◽  
...  
Keyword(s):  
Small Intestine ◽  
Beef Cattle ◽  
Key Words ◽  
Protein Concentration ◽  
Lactase Activity ◽  
Crossbred Cattle ◽  
Tissue Samples ◽  
Intestinal Villi ◽  
Mucosal Membrane ◽  
Mucosal Protein

A study was conducted to determine differences in length of intestinal villi and crypts and activity of mucosal carbohydrases of several breeds of beef cattle. Tissue samples, from three locations in the small intestine, were obtained from heifers and steers of Wagyu (W) × Angus (W × A), W × Hereford (W × H) and W × Holstein (W × O) crossbred cattle and from Holstein, Parthenais and Piedmontese steers at slaughter. In W × A cattle villi were shorter and crypts were longer (P < 0.05) than in cattle of other breeds. Mucosal protein concentration was highest (P < 0.05) in the mid- and distal intestinal regions in the W × A cattle. Maltase activity was highest (P < 0.05) for W × H heifers and least (P < 0.05) for W × A heifers in the proximal intestinal region. Holstein and Piedmontese steers had the highest ADG, and Holstein steers had the highest lactase activity in the mid-intestinal region. Lactase activity in the mid-intestinal region appeared to be weakly associated with ADG of cattle (r = 0.454, P = 0.015, n = 28). These data suggest that differences due to breed of cattle exist in villus and crypt lengths and mucosal carbohydrase activity; however, the influence of these intestinal parameters on cattle performance may be relatively small. Key words: Intestinal villi, crypt, cellobioase, maltase, lactase, beef cattle

THE EFFECT OF FASTING ON DISACCHARIDASE ACTIVITY IN THE RAT SMALL INTESTINE

PEDIATRICS ◽  
1971 ◽  
Vol 47 (1) ◽  
pp. 65-72
Author(s):  
L. K. McNeill ◽  
J. R. Hamilton
Keyword(s):  
Small Intestine ◽  
Epithelial Cells ◽  
Specific Activity ◽  
Cell Mass ◽  
Free Access ◽  
Lactase Activity ◽  
Childhood Diseases ◽  
Mucosal Protein ◽  
And Function ◽  
Fasted Rats

We assessed intestinal structure, mucosal epithelial kinetics, and disaccharidase activities after fasting. Rats fasted for up to 120 hours were compared with control rats fed ad libitum. All rats had free access to water and all were prevented from ingesting their own stools. Body weight, small intestinal weight and mucosal protein, and maltase and sucrase activity of the total small intestine decreased in fasted rats. Lactase activity did not decrease. Specific activity of lactase actually increased in the jejunum. Assessed after a 96-hour fast, jejunal villi were shortened with less epithelial cells along their length and the rate of migration of those cells along the villi was diminished in the fasted rats compared with control rats. We attribute the decreased total intestinal sucrase and maltase activities to a loss of total epithelial cell mass in the small bowel. An abnormality in the cells of the progenitor zone of the crypts is suggested by the decreased migration rate of mucosal epithelial cells in fasting rats. These factors do not explain our observations completely since lactase activity did not diminish. We postulate that the activity of the "acid" β-galactosidase located in the cytoplasm or lysosomes of the epithelial cells was stimulated by fasting. Our observations are relevant to clinical pediatrics. Undernutrition and fasting my be associated with many childhood diseases and with treatment of disease. In assessing clinical data and advising treatment, the pediatrician should be aware of the potentially harmful effects of starvation on intestinal structure and function.

scholarly journals MICROSCOPIC STRUCTURE OF ALBINO RATS’ SMALL INTESTINE

2019 ◽  
Vol 72 (5) ◽  
pp. 733-738 ◽  
Author(s):  
Volodymyr H. Hryn ◽  
Yuriy P. Kostylenko ◽  
Valentyna P. Bilash ◽  
Olena B. Ryabushko
Keyword(s):  
Small Intestine ◽  
Histological Study ◽  
Experimental Studies ◽  
Specific Pattern ◽  
Male Rats ◽  
Albino Rats ◽  
Histological Structure ◽  
Tissue Samples ◽  
Thin Sections ◽  
Intestinal Villi

Introduction: The small intestine of albino rats is a transitive canal between the stomach and the cecum that is closely located from each other, reaches a length of one meter, which in comparison ratio to body weight significantly exceeds the corresponding segment in humans. The aim: The paper is aimed at thorough histological study of the wall and structure of albino rats’ small intestine mucosa. Materials and methods: 30 mature albino male rats were involved into the study. The specimens of albino rats’ small intestine, fixed in 10% neutral buffered formalin solution, have been studied. The study was carried out using conventional histological methods for obtaining serial paraffin sections stained with hematoxylin-eosin. Epoxy plastination of individual tissue samples of the small intestine was performed. Subsequently, polished thin sections were made, stained with 1% methylene blue and 1% borax solution. The obtained specimens were studied on the “Konus” light microscope equipped with Sigeta DCM-900 9.0MP digital microphoto attachment with the Biorex 3 software adapted for studies of such type. Results and conclusions: For the first time in the practice of histological study of the epithelial covering of the mucous membrane of the small intestine, attention is drawn to the specific pattern of its organization on the intestinal villi. It has been found that epithelial covering consists of alternating cluster epithelial aggregations separated by fissured depressions. Since no mentioning about them has been found in the publication, these cluster aggregations of enterocytes can be called epithelial buds of the intestinal villi. Consequently, it can be concluded that with the exception of some specific morphological features, the small intestine of albino rats is homologous to human one by its histological structure, which means that it can be used as a model for various experimental studies.

Changes in rat mesentery interstitial matrix due to superfusate

1993 ◽  
Vol 265 (3) ◽  
pp. H852-H856 ◽  
Author(s):  
B. J. Barber ◽  
R. A. Babbitt ◽  
S. Dutta ◽  
S. Parameswaran
Keyword(s):  
Protein Content ◽  
Normal Saline ◽  
Protein Concentration ◽  
Matrix Protein ◽  
Protein Transport ◽  
Albumin Concentration ◽  
Sprague Dawley ◽  
Tissue Samples ◽  
Rat Mesentery ◽  
Sprague Dawley Rats

Animal preparations for microscopy often require a superfusate solution to cover surgically exposed tissue. There are few, if any, data concerning the effects of this solution on extravascular protein concentration and hydration. The effect of superfusion on mesenteric tissue in anesthetized male Sprague-Dawley rats was studied. Tissue samples were taken from nonsuperfused and superfused tissue and analyzed for hydration, albumin, and transferrin content. The mesenteric tissue interstitial matrix was rapidly altered by normal saline superfusate. After superfusion, there was a decrease (P < 0.01) in tissue albumin concentration from 1.17 +/- 0.27 to 0.10 +/- 0.08 g/dl (n = 9). Tissue hydration increased from 4.98 +/- 0.8 micrograms water/microgram dry wt in controls to 7.38 +/- 1.2 micrograms water/micrograms dry wt after superfusion. When a range of superfusate albumin concentrations was used (0, 1, 2, and 3 g/dl), tissue albumin concentration changed 0.59 +/- 0.09 g/dl for each gram per deciliter change in superfusate concentration (P < 0.0001). The large changes in interstitial matrix protein content and hydration suggest that superfusate solution effects need to be considered in microvascular protein transport experiments.

Effect of feeding barley based diets on animal performance, carcass characteristics and meat quality of crossbred beef cattle with and without Wagyu genetics

1997 ◽  
Vol 77 (4) ◽  
pp. 655-662 ◽  
Author(s):  
P. S. Mir ◽  
D. R. C. Bailey ◽  
Z. Mir ◽  
S. D. M. Jones ◽  
T. Entz ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Meat Quality ◽  
Shear Force ◽  
Average Daily Gain ◽  
Carcass Characteristics ◽  
Barley Grain ◽  
Crossbred Cattle ◽  
Target Weight ◽  
Meat Samples

Growth performance, carcass characteristics and meat quality of European and British crossbred (EBC; no Wagyu genetics; 28 heifers and 30 steers) cattle were compared with crossbred cattle with 75% Wagyu genetics (WC; seven heifers and 14 steers) to determine the influence of Wagyu genetics on marbling grade of beef cattle fed barley-based diets in a factorial design experiment. Weaned calves (250 d average age) were fed, one of two diets (diet 1, 35% barley grain; diet 2, 40% hay cubes on DM basis, with barley silage, protein and vitamin/mineral premix) for 84 d and then fed diet 1 until they weighed 394 to 432 kg. All cattle were finished on an 80% (DM basis) rolled barley diet and slaughtered. Carcasses were graded and samples procured for meat quality and Warner–Bratzler shear force determination. Number of days on backgrounding diets to arrive at target weight (380 kg) was greater (P < 0.05) for the WC cattle, owing to relatively lower ADG, but days on the finishing diet were fewer for these cattle, compared with EBC cattle. Warm carcass yield (dressing percent) was greater (P < 0.05), but backfat depth was lower (P < 0.05) for WC cattle relative to that of EBC cattle, yet proportion of lean meat yield was similar. Eighty three percent of WC cattle carcasses had Canada AAA (small or more) marbling grade compared with 13% for EBC cattle carcasses. Mean shear force of meat samples from EBC and WC cattle was 4.2 and 3.8 kg, respectively. Results indicated that the extent of carcass marbling can be increased by incorporating Wagyu genetics but age at slaughter of WC cattle was 19 d greater than that of EBC cattle and carcass size was reduced. Key words: Average daily gain, carcass characteristics, European and British crossbred, feed to gain ratio, meat quality, Wagyu crossbred cattle

Mechanical properties of the tracheal mucosal membrane in the rabbit. II. Morphometric analysis

2000 ◽  
Vol 88 (3) ◽  
pp. 1022-1028 ◽  
Author(s):  
Lu Wang ◽  
Kenneth L. Pinder ◽  
Joel L. Bert ◽  
Mitsushi Okazawa ◽  
Peter D. Paré
Keyword(s):  
Mechanical Properties ◽  
Volume Fraction ◽  
Mechanical Load ◽  
Morphological Structure ◽  
Longitudinal Direction ◽  
Smooth Muscle Contraction ◽  
Elastic Fibers ◽  
Tissue Samples ◽  
Mucosal Membrane ◽  
Simple Geometry

Folding of the airway mucosal membrane provides a mechanical load that impedes airway smooth muscle contraction. Mechanical testing of rabbit tracheal mucosal membrane showed that the membrane is stiffer in the longitudinal than in the circumferential direction of the airway. To explain this difference in the mechanical properties, we studied the morphological structure of the rabbit tracheal mucosal membrane in both longitudinal and circumferential directions. The collagen fibers were found to form a random meshwork, which would not account for differences in stiffness in the longitudinal and circumferential directions. The volume fraction of the elastic fibers was measured using a point-counting technique. The orientation of the elastic fibers in the tissue samples was measured using a new method based on simple geometry and probability. The results showed that the volume fraction of the elastic fibers in the rabbit tracheal mucosal membrane was ∼5% and that the elastic fibers were mainly oriented in the longitudinal direction. Age had no statistically significant effect on either the volume fraction or the orientation of the elastic fibers. Linear correlations were found between the steady-state stiffness and the quantity of the elastic fibers oriented in the direction of testing.

scholarly journals Pemotongan Sapi Betina Produktif di Rumah Potong Hewan di Daerah Istimewa Yogyakarta

2016 ◽  
Vol 7 (1) ◽  
pp. 20
Author(s):  
Nur Rasminati
Keyword(s):  
Beef Cattle ◽  
Key Words

<div class="WordSection1"><p><em>The research was conducted to know the number of the productive female beef cattle slaughtering at abattoir in DIY. All of the beef cattle which slaughtered along a month were used in this research. The result showed that the number of productive beef cattle slaughtered  up to 2 years old were 28.98%; 2 – 5 years old were 69.55%; more than 5 years old were 1.44%. The female beef cattle slaughtered were 54.58% and the male beef cattle slaughtered were 32.36%. The non productive beef cattle slaughtered were 13.04% including 5.79% male and 7.24% female. The Peranakan Ongole (PO) breed slaughtered was 60.36% (125 heads), Brahman Cross 16.42% (34 heads), Simmental 15.94% (33 heads), Brangus 2.42% (5 heads), Limousine 4.35% (9 heads) and PFH 0.48% (1 heads). The percentage of productive beef cattle slaughtering were 86.95% with female beef productive were 64.25% and the PO breed at 2 – 5 years old were the most slaughtered. It could be concluded that the productive female beef cattle slaughtering at abattoir in DIY was quite high, which may influence the breed resources of beef cattle.</em></p><p><em> </em></p><p><em>Key words : Productive beef cattle, slaughtering, abattoir, Yogyakarta</em></p></div><em><br clear="all" /> </em>

scholarly journals Determination of CYP450 Expression Levels in the Human Small Intestine by Mass Spectrometry-Based Targeted Proteomics

2021 ◽  
Vol 22 (23) ◽  
pp. 12791
Author(s):  
Alexia Grangeon ◽  
Valérie Clermont ◽  
Azemi Barama ◽  
Fleur Gaudette ◽  
Jacques Turgeon ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Small Intestine ◽  
Protein Expression ◽  
Mrna Levels ◽  
Targeted Proteomics ◽  
Tissue Samples ◽  
Human Organ ◽  
Expression Levels ◽  
Protein Levels ◽  
Human Small Intestine

The human small intestine can be involved in the first-pass metabolism of drugs. Under this condition, members of the CYP450 superfamily are expected to contribute to drug presystemic biotransformation. The aim of this study was to quantify protein expression levels of 16 major CYP450 isoforms in tissue obtained from nine human organ donors in seven subsections of the small intestine, i.e., duodenum (one section, N = 7 tissue samples), jejunum (three subsections (proximal, mid and distal), N = 9 tissue samples) and ileum (three subsections, (proximal, mid and distal), N = 9 tissue samples), using liquid chromatography tandem mass spectrometry (LC-MS/MS) based targeted proteomics. CYP450 absolute protein expression levels were compared to mRNA levels and enzyme activities by using established probe drugs. Proteins corresponding to seven of sixteen potential CYP450 isoforms were detected and quantified in various sections of the small intestine: CYP2C9, CYP2C19, CYP2D6, CYP2J2, CYP3A4, CYP3A5 and CYP4F2. Wide inter-subject variability was observed, especially for CYP2D6. CYP2C9 (p = 0.004) and CYP2C19 (p = 0.005) expression levels decreased along the small intestine. From the duodenum to the ileum, CYP2J2 (p = 0.001) increased, and a trend was observed for CYP3A5 (p = 0.13). CYP3A4 expression was higher in the jejunum than in the ileum (p = 0.03), while CYP4F2 expression was lower in the duodenum compared to the jejunum and the ileum (p = 0.005). CYP450 protein levels were better correlated with specific isoform activities than with mRNA levels. This study provides new data on absolute CYP450 quantification in human small intestine that could improve physiologically based pharmacokinetic models. These data could better inform drug absorption profiles while considering the regional expression of CYP450 isoforms.

Mechanism of glucagon-induced intestinal secretion

1980 ◽  
Vol 239 (1) ◽  
pp. G30-G38
Author(s):  
D. N. Granger ◽  
P. R. Kvietys ◽  
W. H. Wilborn ◽  
N. A. Mortillaro ◽  
A. E. Taylor
Keyword(s):  
Capillary Pressure ◽  
Plasma Proteins ◽  
Fluid Pressure ◽  
Intestinal Secretion ◽  
Tissue Samples ◽  
Mucosal Membrane ◽  
Osmotic Reflection Coefficient ◽  
Capillary Fluid ◽  
Higher Doses ◽  
Flow Capillary

The effects of local intra-arterial glucagon infusion on transcapilary, lymphatic, and transmucosal fluid and protein fluxes were studied in autoperfused segments of cat ileum. The glucagon infusions resulted in a significant increase in intestinal blood flow, lymph flow, capillary filtration coefficient, capillary pressure, interstitial volume, and interstitial fluid pressure. Precapillary resistance and the pre-to-postcapillary resistance ratio decreased during the glucagon infusion. The transcapillary oncotic pressure gradient and the osmotic reflection coefficient were reduced, suggesting that capillary permeability is significantly increased with glucagon. Ultrastructural analysis of tissue samples acquired during the infusion of higher doses of glucagon indicates disruption of the mucosal membrane. An glucagon indicates disruption of the mucosal membrane. An alteration in mucosal structure is supported by the appearance of plasma proteins in the secreted fluid. The results of this study indicate that glucagon-induced intestinal secretion results from an alteration in capillary fluid balance, i.e., an increased capillary pressure and permeability.

Associations of melanocortin 1 receptor genotype with growth and carcass traits in beef cattle

2009 ◽  
Vol 89 (3) ◽  
pp. 295-300 ◽  
Author(s):  
Kim L McLean ◽  
Sheila M Schmutz
Keyword(s):  
Beef Cattle ◽  
Key Words ◽  
Coat Colour ◽  
Longissimus Dorsi ◽  
Red Pigment ◽  
Melanocortin 1 Receptor ◽  
Melanocyte Stimulating Hormone ◽  
Melanocortin 4 Receptor ◽  
Main Gene ◽  
Fat Level

Melanocortin 1 Receptor (MC1R) is considered to be the main gene controlling the production of eumelanin or phaeomelanin, resulting in black or red coat colour of cattle. The recessive red allele, e, codes for a nonfunctional receptor, which does not bind the agonist alpha-melanocyte stimulating hormone (α-MSH), allowing for the production of phaeomelanin, or red pigment, whereas the dominant ED allele binds α-MSH leading to the production of eumelanin. We hypothesized that black cattle would have more α-MSH bound to MC1R, which could result in more α-MSH binding to the appetite suppressing receptor, Melanocortin 4 Receptor. We genotyped 328 crossbred steers of various colours that were purchased at weaning and fed until slaughter. Black cattle of ED/ED or ED/e genotype had increased back fat and required significantly fewer days (15-25) on feed to reach a target fat level for slaughter than the red cattle. Red cattle of e/e genotype were found to have a significantly larger longissimus dorsi (l. dorsi), shipping weight and hot carcass weight. Differences were comparable whether black versus red coat colour or MC1R genotype were used as the criteria for the group of cattle. Key words: Coat colour, MC4R, MC1R, α-MSH, shipping weight

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