scholarly journals Antibacterial properties and preliminary phytochemical analysis of methanolic extract of Ocimum gratissium (Scent Leaves)

2011 ◽  
Vol 3 (2) ◽  
Author(s):  
FO Ekhaise ◽  
AE Soroh ◽  
A Falodun
Keyword(s):  
Methanolic Extract ◽  
Antibacterial Properties ◽  
Phytochemical Analysis

FINGER PRINTING ANALYSIS OF THE PHENOLS FROM HOLOPTELEA INTEGRIFOLIA (ROXB.) PLANT LEAVES USING HPTLC

INDIAN DRUGS ◽  
2017 ◽  
Vol 54 (09) ◽  
pp. 67-71
Author(s):  
R. C. Sutar ◽  
◽  
D. S Musmade
Keyword(s):  
High Performance ◽  
Methanolic Extract ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Plant Leaves ◽  
Methanol Extracts ◽  
Finger Printing ◽  
Rf Values ◽  
Layer Chromatography ◽  
Tungsten Lamp

The present study was conducted to identify the phenols from methanol extracts (MHI) of medicinally and economically useful leaves of Holoptelea integrifolia (Roxb.) plant using High Performance Thin Layer Chromatography (HPLC) technique. Preliminary phytochemical screening was done and HPTLC studies were carried out on CAMAG HPTLC system equipped with Linomat V applicator (Switzerland). Densitometric scanning was performed with Camag TLC scanner IV in the reflectance absorbance mode at 540 nm and operated by Win CATS software (1.4.6 Camag) with the help of tungsten lamp. Preliminary phytochemical screening of methanolic extract of Holoptelea integrifolia showed the presence of steroids, alkaloids, flavonoids, proteins, phenols and carbohydrates. HPT LC finger printing of phenols of methanolic extract of leaf revealed seven polyvalent phytoconstituents (7 peaks) and corresponding ascending order of Rf values in the range of 0.15 to 0.75. From the results of preliminary phytochemical analysis and above Rf values, we have concluded the presence of phenols in methanol extracts.

scholarly journals SCREENING FOR PHYTOCHEMICALS AND FTIR ANALYSIS OF MYRISTICA DACTYLOIDS FRUIT EXTRACTS

2016 ◽  
Vol 9 (1) ◽  
pp. 315 ◽  
Author(s):  
P. Rajiv ◽  
A. Deepa ◽  
P. Vanathi ◽  
D. Vidhya
Keyword(s):  
Fourier Transform ◽  
Methanolic Extract ◽  
Phytochemical Analysis ◽  
Ftir Analysis ◽  
Spectroscopy Analysis ◽  
Standard Methods ◽  
Fruit Extracts ◽  
Ft Ir ◽  
Amine Groups ◽  
Ir Analysis

<p><strong>Objective: </strong>The present investigation focus on screening of phytochemicals and FT-IR analysis of <em>Myristica dactyloids </em>fruit extracts. The fruit extracts were prepared using five different solvents.</p><p><strong>Methods: </strong>The phytochemical analysis and FT-IR (Fourier transform infrared spectroscopy) analysis were performed using standard methods.</p><p><strong>Results: </strong>The results reveals that the alkaloids, steroids, flavonoids, phenolic compounds, proteins, carbohydrates, cardio glycosides and saponins were present in methanolic extract when compared to other solvent extracts. FT-IR analysis shows the presence of different functional groups such as carboxylic acids, aromatics, alkanes, alcohols, phenols, aliphatic amines, alkenes and amine groups in the fruit extracts.</p><p><strong>Conclusion: </strong>The study concluded that the methanolic extract (<em>M. dactyloides </em>fruit) has potential bioactive compounds.</p>

scholarly journals Study on antibacterial activity of Calotropis procera

2014 ◽  
Author(s):  
Muhammad Muzammal
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Methanolic Extract ◽  
Ethanolic Extract ◽  
Salmonella Typhi ◽  
Calotropis Procera ◽  
Phytochemical Analysis ◽  
Zone Of Inhibition ◽  
Crude Extracts ◽  
Mic Value

Comparative study of plant extracts crude and aqueous, methanolic and ethanolic with antibiotics, provide evidence that calotropis procera extracts has the similar antibacterial activity as these antibiotics against test pathogens i.e. Salmonella typhi and E.coli. The analysis of antimicrobial activity of aqueous, methanolic and ethanolic extract of leaves and flower of Calotropis procera was carried out in disc method and also determined MIC value at 600nm through optical density using spectrophotometer. The zone of inhibition produced by extracts was examined and compares it with zone produced by antibiotics. The effect exhibited by ethanolic extract of leaves and flower was significantly greater than the aqueous and methanolic extract of leaves and flower. Crude extracts i.e. latex, leaves, fruit and flower crude extracts. Among them, flower crude extracts shows similar zone of inhibition to test anitibiotics.while in MIC value, we made different concentration of extracts and antibiotics.i.e for crude we made 25%, 50%.75% and 100% concentration of crude juice and for the aqueous, methanolic and ethanolic we made 0.5mg/mL,1mg/mL,2mg/mL,3mg/mL,4mg/mL, 5mg/mL and 6mg/mL in Dimethyl salfoxide, and same antibiotics concentration. Aqueous leaves extracts show MIC at 0.5mg/ml against E.coli while against Salmonella it shows MIC at 1mg/ml... We also determine phytochemical analysis for presences of different compounds in crude extracts. The obtained results provide a support for the use of Calotropis procera, in traditional medicine and suggest its further advance investigation.

scholarly journals Histopathological Studies on the Effects of Chloroform and Methanolic Extracts of Ilex kudingcha in Trypanosoma brucei Infected Albino Wistar Rats

2018 ◽  
Vol 04 ◽  
pp. 50
Author(s):  
Olajoju. T. Soniran ◽  
Kalu. K. Ngele ◽  
Christopher. O. Alisa ◽  
Damilola. A. Omoboyowa ◽  
Nnabude. H. Agu ◽  
...  
Keyword(s):  
Trypanosoma Brucei ◽  
Wistar Rats ◽  
Methanolic Extract ◽  
Phytochemical Analysis ◽  
Diminazene Aceturate ◽  
Leaf Extracts ◽  
Standard Drug ◽  
Phytochemical Study ◽  
Reference Drug ◽  
Histopathological Studies

Histopathological studies of the effects of chloroform and methanolic leaf extracts of Ilex kudingcha in Trypanosoma brucei infected albino wistar rats were investigated. The toxicity and phytochemical study were also carried out using standard protocol. T. brucei infected animals were administered orally with 200 and 400 mg/kg b.w. of the extracts and 3.5 mg/kg b.w. of the standard drug (diminazene aceturate). Results on acute toxicity studies (LD50) revealed no sign of lethality up to the dose of 5,000 mg/kg body weight but the liver and kidney histology of infected animals treated with 5,000 mg/kg b.w. of I. kudingcha extracts were observed to be hepatotoxic and nephrotoxic. The methanol extracts showed appreciably high in vivo anti-trypanosomal activities compared to the reference drug. Histological examination of the organs revealed serious pathological lesions in the liver of the infected animals without treatment (negative control). In the positive control animals (infected animals administered standard drug), mild multifocal aggregate of inflammatory leucocytes was observed. In the other experimental animals, no pathological lesion was observed in the liver, kidney, brain, and heart of infected animals treated with the methanolic extract and combined methanol and chloroform extracts. The effectiveness of the methanolic extract at reducing the lesions caused by the parasite is the same compared with the standard drug. Phytochemical analysis of the plant extracts showed that methanol extract contained appreciable high levels of alkaloids, saponin, tannins, phenol, and glycoside while flavonoid was not detected. Hence, the curative properties of methanolic extract of I. kudingcha as observed in the organs indicate its anti-trypanosomal properties but it should be consumed at minimal doses.

scholarly journals ANTI-BIOFILM POTENTIAL OF MENTHOL PURIFIED FROM MENTHA PIPERITA L. (MINT)

2020 ◽  
Vol 2020 (1) ◽  
Author(s):  
R Ejaz ◽  
S Malik ◽  
M Ahmad ◽  
H ALi ◽  
S Choudhry
Keyword(s):  
Column Chromatography ◽  
Antibacterial Properties ◽  
Ethanolic Extract ◽  
Bioactive Compound ◽  
Solvent System ◽  
Mentha Piperita ◽  
Phytochemical Analysis ◽  
Antibacterial Drug ◽  
Biofilm Inhibition ◽  
Maximum Separation

Menthol, a bioactive compound of Mentha piperita (mint) with antibacterial properties was purified by column chromatography to determine its anti-biofilm potential. After phytochemical analysis, TLC was carried out using n-hexane: ethyl acetate: methanol: water (2:2:2:1) as the solvent system for ethanolic extract of mint. TLC achieved the maximum separation of mint constituents with Rf value of 0.68. A purified menthol fraction was obtained after silica gel column chromatography using four different eluting solvents. The menthol obtained was then used to perform biofilm inhibition assay to establish its antibacterial potential. Percentage inhibition was highest for bacillus subtilis (79.4%), as opposed to Pseudomonas aeruginosa (33.6%) and the combination of both bacteria (20%). ELISA reader was used to measure absorbance at 450-620nm and 630 nm. Using 450-620nm filter the values for percentage inhibition lies between 48.6-95% for standard and crude menthol samples. Similarly, at 630nm the values of inhibition lie between 23.4-70.6%. This anti-biofilm property of menthol can be utilized in antibacterial drug formulations.

scholarly journals ENIGMATIC INDUCTION OF CYTOMIXIS IN ALLIUM CEPA ROOT MERISTEM BY AGLAIA EDULIS ROXB. LEAF EXTRACT AND ITS PHYTOCHEMICAL RATIONALE

2020 ◽  
pp. 168-171
Author(s):  
ARCHANA ELAMKULAM RAVINDRAN ◽  
JOHN ERNEST THOPPIL
Keyword(s):  
Allium Cepa ◽  
Leaf Extract ◽  
Root Meristem ◽  
Methanolic Extract ◽  
Synergistic Action ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Phytochemical Analysis ◽  
Spectrometry Analysis ◽  
Dose Dependent

Objective: The present study aims to analyze the potential of Aglaia edulis Roxb. leaf extract to induce cytological aberrations in Allium cepa root meristem and to determine the phytoconstituents in the extract. Methods: Cytotoxicity evaluation of the leaf methanolic extract was done using Allium cepa assay using various concentrations. Volatile phytoconstituents in the extract were determined using gas chromatography–mass spectrometry analysis. Results: Considerable number of cytomictic cells along with other aberrations was observed. The occurrence of cytomixis was found to be dose dependent where it ranged from 6.58±0.35 to 29.45±0.45. The percentage of cytomictic cells among the total aberrant cells was observed between 35.19±1.67 and 77.39±1.39. The phytochemical analysis of the plant extract revealed the presence of active secondary metabolites. Conclusion: The synergistic action of the active compounds might have triggered the phenomenon of cytomixis which, in turn, could be exploited for the production of polyploids.

scholarly journals Phytochemical Analysis and Biological Investigation of Nepeta juncea Benth. Different Extracts

Plants ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 646 ◽  
Author(s):  
Majid Sharifi-Rad ◽  
Francesco Epifano ◽  
Serena Fiorito ◽  
José M. Álvarez-Suarez
Keyword(s):  
Inhibitory Concentration ◽  
Methanolic Extract ◽  
Radical Scavenging ◽  
Dry Weight ◽  
Antibacterial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Phytochemical Analysis ◽  
Biological Investigation ◽  
First Time ◽  
Dpph Radical Scavenging

This study was carried out to screen the amount and the classes of secondary metabolites and to evaluate the antioxidant, cytotoxic, antifungal, and antibacterial activities of the methanolic, ethanolic, and water extracts of the roots, leaves, and flowers of Nepeta juncea Benth. The results show that the highest total phenol (69.54 ± 0.31 mg gallic acid equivalents (GAE)/g dry weight), total flavonoid (41.37 ± 0.17 mg quercetin equivalents (QE)/g dry weight), anthocyanin (6.52 ± 0.21 mg cyanidin/100 g dry weight), and tannin (47.36 ± 0.33 mg catechin/g dry weight) concentrations were recorded in the methanolic extract of the leaves of N. juncea. The gas chromatography–mass spectrometry (GC–MS) analysis of the extracts showed that 1,8-cineole, 4aα-7α-7aα-nepetalactone, β-pinene, terpinen-4-ol, and α-terpineol were the major compounds, respectively. The best 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and ferric-reducing antioxidant, cytotoxic, antifungal, and antibacterial activities were observed for the methanolic extract of the leaves. For the two latter activities, the best activity was revealed on Staphylococcus aureus, Bacillus cereus, and Candida albicans. The minimum inhibitory concentration (MIC) values for the antimicrobial of the methanolic extract from the leaves were in the range of 25–100 µg/mL, whereas the minimum bactericidal concentration (MBC) values were in the range of 50–200 µg/mL. The results reported herein show that, for the first time in the literature, N. juncea is a remarkable source of antioxidant, antifungal, and antibacterial compounds.

scholarly journals An Alkenylresorcinol Derivative from Hakea Sericea Fruits and their Antimicrobial Activity

2013 ◽  
Vol 8 (10) ◽  
pp. 1934578X1300801 ◽  
Author(s):  
Ângelo Luís ◽  
Carla Cruz ◽  
Ana Paula Duarte ◽  
Fernanda Domingues
Keyword(s):  
Methanolic Extract ◽  
High Resolution Mass Spectrometry ◽  
Antibacterial Properties ◽  
Gram Negative Bacteria ◽  
Two Dimensional ◽  
Shrub Species ◽  
Phytochemical Composition ◽  
Bioassay Guided Fractionation ◽  
Mrsa Strains ◽  
Microtiter Assay

Hakea sericea has been introduced to Portugal for ornamental purposes. The phytochemical composition and the antioxidant, antibacterial, antibiofilm and cytotoxic properties of this shrub species have been previously reported. The present work describes the bioassay-guided fractionation of the crude methanolic extract of H. sericea fruits and the isolation of 9-(3,5-dihydroxy-4-methylphenyl)nona-3( Z)-enoic acid. The structure of this new compound was established by one- and two-dimensional NMR and IR spectroscopy, and high-resolution mass spectrometry. The antibacterial properties of the new alkenylresorcinol were studied by determining its MIC values against several strains of Gram-positive and Gram-negative bacteria using the resazurin microtiter assay. The new alkenylresorcinol inhibited the growth of Enterococcus faecalis, Listeria monocytogenes and Bacillus cereus with MIC values of 0.31, 0.02 and 0.16 mg/mL, respectively. Good MIC values were obtained against Staphylococcus aureus strains (0.005 – 0.16 mg/mL), including the clinical isolates (SA 01/10, SA 02/10 and SA 03/10) and MRSA strains.

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