scholarly journals Activités antioxydante et insecticide d’huiles essentielles de Mentha arvensis L. du Sénégal

2021 ◽  
Vol 15 (3) ◽  
pp. 966-975
Author(s):  
Serigne Mbacké Diop ◽  
Momar Talla Gueye ◽  
El Hadji Barka Ndiaye ◽  
Abdoulaye Thiam ◽  
Papa Seyni Cissokho ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Essential Oils ◽  
Sitophilus Zeamais ◽  
Mentha Arvensis ◽  
Composition Chimique ◽  
Global Context ◽  
Flame Ionization ◽  
Dpph Method ◽  
Sitophilus Zeamais Motsch ◽  
Insecticidal Activities

Ce travail s'inscrit dans le contexte global de valorisation de la biodiversité des plantes aromatiques du Sénégal. L’objectif de l’étude était de déterminer la composition chimique d’huiles essentielles (HE) de Mentha arvensis L. et d’évaluer leurs activités antioxydante et insecticide. A cet effet, des feuilles de M. arvensis ont été récoltées dans la région de Dakar (Sénégal) et séchées à l'ombre puis leurs HE extraites par entrainement à la vapeur d'eau. Les HE obtenues ont été analysées par chromatographie en phase gazeuse (CPG) couplée à un détecteur à ionisation de flamme (FID) et un spectromètre de masse (MS). L’activité antioxydante des HE a été évaluée par la méthode d’inhibition du radical 2,2-diphényl-1-picrylhydrazyl (DPPH) tandis que l’activité insecticide a été testée par fumigation contre Sitophilus zeamais (Motsch.). Les résultats ont montré que les HE contiennent principalement du menthol (68,4%), de l’acétate de menthyle (16,9%) et de la menthone (5,0%). Leur activité antioxydante augmente avec la concentration, soient 38%, 57% et 73% d’inhibition respectivement pour 10, 25 et 50 mg/ml ; la CI50 est de 19,5 mg/ml. Les huiles présentent aussi une activité insecticide. Cette dernière bien qu’étant faible après 24 heures, augmente fortement au bout de 48 heures avec une mortalité maximale de 92%. Cette mortalité atteint des maximums de 98% pour 5 et 25 μl et 100% pour 50 et 75 μl après 72 heures.   English title: Antioxidant and insecticidal activities of essential oils of Mentha arvensis L. from Senegal This work is part of the global context of valorization of the biodiversity of aromatic plants from Senegal. The aim of the study is to determine the chemical composition of essential oils (EOs) of Mentha arvensis L. and to evaluate their antioxidant and insecticidal activities. For this purpose, leaves of M. arvensis were harvested in Dakar region, Senegal and dried in the shade. EOs were extracted by steam distillation from dried leaves and analyzed by gas chromatography (GC) coupled to a flame ionization detector (FID) and a mass spectrometer (MS). The antioxidant activity of EOs was assessed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method while the insecticidal activity was tested by fumigation against Sitophilus zeamais (Motsch.). The results revealed that EOs were dominated by menthol (68.4%), menthyl acetate (16.9%) and menthone (5.0%). The antioxidant activity assay showed that the inhibition increased with the concentration: 38%, 57% and 73% for 10, 25 and 50 mg/ml, respectively; the IC50 was of 19.5 mg/ml. The oils were also effective as insecticide. Mortalities were low after 24 hours and increased strongly after 48 hours with a maximum of 92%. They reached a maximum of 98% for 5 and 25 μl and 100% for 50 and 75 μl after 72 hours.    

scholarly journals Effects of Essential Oils from 24 Plant Species on Sitophilus zeamais Motsch (Coleoptera, Curculionidae)

Insects ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 532
Author(s):  
William R. Patiño-Bayona ◽  
Leidy J. Nagles Galeano ◽  
Jenifer J. Bustos Cortes ◽  
Wilman A. Delgado Ávila ◽  
Eddy Herrera Daza ◽  
...  
Keyword(s):  
Essential Oils ◽  
Chemical Control ◽  
Sitophilus Zeamais ◽  
Contact Toxicity ◽  
Stored Products ◽  
Collateral Damage ◽  
Maize Weevil ◽  
Active Constituents ◽  
Insect Mortality ◽  
Sitophilus Zeamais Motsch

Chemical control of the maize weevil (Sitophilus zeamais) has been ineffective and presents serious collateral damage. Among plant-derived insecticides, essential oils (EOs) are suitable candidates to control this stored products pest. In this work, the insecticidal activities of 45 natural EOs against S. zeamais adults were screened, and the most promising ones (24 EOs) were characterized by GC–MS. The repellent and toxic effects (contact and fumigant) of these 24 EOs were determined, and by a cluster analysis they were classified into two groups considering its fumigant activity and contact toxicity. For the EOs with the highest fumigant potential (14 oils) and their main active constituents (17 compounds), lethal concentrations were determined. The most active EOs were those obtained from L. stoechas and L. alba, with LC50 values of 303.4 and 254.1 µL/L air and characterized by a high content of monoterpenes. Regarding the major compounds, the oxygenated monoterpenes R-(+)-pulegone (LC50 = 0.580 mg/L air), S-(-)-pulegone (LC50 = 0.971 mg/L air) and R-(-)-carvone (LC50 = 1.423 mg/L air) were the most active, as few variations in their concentrations significantly increased insect mortality.

Comparative Study of Yield, Chemical Composition and Antioxidant Activity of Wild Algerian Lavandula stoechas L. Obtained by Ultrasound Pre-treatment and by Conventional Hydrodistillation

2018 ◽  
Vol 16 (S1) ◽  
pp. S109-S118 ◽  
Author(s):  
L. Chekoual ◽  
A. Aissat ◽  
K. Ait-Kaci Aourahoun ◽  
T. Benabdelkader
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Treatment Time ◽  
Quantitative Difference ◽  
Flame Ionization ◽  
Lavandula Stoechas ◽  
Two Samples ◽  
Pre Treatment ◽  
Two Populations

The paper presents research results concerning the influence of ultrasonic pre-treatment (time intervals of 10, 20, 30, 45, and 60 min) on the process of extraction of essential oils from wild Algerian Lavandula stoechas. Tests were carried out in two populations. A sonication pre-treatment time of 10 min followed by 90 min of hydrodistillation (US-HD) gives a yield of 1.60 ± 0.1% of Kodiat essential oils (KEO). This pre-treatment time proved to be the best when compared with other pretreatment times. However, overall best yield (1.03 ± 0.07%) of El-Kahla essential oils (EEO) was obtained at 60 min of pre-treatment. These yields are higher compared with those (0.64 ± 0.02% and 0.50 ± 0.02%) obtained after 180 min of only hydrodistillation (HD) for the two samples of Lavandula stoechas, respectively. About 90.69% and 89.36% of total compounds were identified using chromatography–mass spectrometry (GC– MS) in KEO and EEO treated by ultrasound versus untreated samples (89.26% and 76%, respectively). A qualitative and quantitative difference in chemical composition between the essential oils of treated and untreated samples of the two harvesting sites was found. The percentage of most of the major compounds (fenchone, camphor, 1,8-cineole, bornylacetate, myrtenyl-acetate, and viridiflorol) and other compounds identified is higher in treated samples. GC/FID (flame ionization detector) analysis of KEO showed that the percentage of fenchone and camphor as major components is greater at 60 and 20 min of ultrasonic (US) pretreatment, respectively, compared with HD and the other US pre-treatment times. Results of the present study clearly indicated that sonication treatments are effective in enhancing the antioxidant activity of Lavandula stoechas extracts and could be further explored for commercial purposes to benefit consumers and the environment.

scholarly journals Free Volatile Compounds of Veronica austriaca ssp. jacquinii (Baumg.) Eb. Fisch. and Their Biological Activity

Plants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2529
Author(s):  
Marija Nazlić ◽  
Željana Fredotović ◽  
Elma Vuko ◽  
Nenad Vuletić ◽  
Ivica Ljubenkov ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Essential Oils ◽  
Volatile Compounds ◽  
Glandular Trichomes ◽  
Principal Component ◽  
Stalk Cell ◽  
Total Phenolic ◽  
Dpph Method ◽  
Hexahydrofarnesyl Acetone ◽  
Different Types

The composition of free volatile compounds of essential oils (EO) and hydrosols (Hy) from four different localities of the species Veronica austriaca ssp. jacquinii (Baumg.) Eb. Fisch. were analyzed by gas chromatography coupled with mass spectrometry. In the EOs, the most abundant compounds identified were hexahydrofarnesyl acetone (23.34–52.56%), hexadecanoic acid (palmitic acid, 26.71–58.91%) and octadecanol acetate (0–6.24%). The hydrosols were characterized by high abundance of methyl eugenol (23.35–57.93%), trans-p-mentha-1(7),8-dien-2-ol (5.24–7.69%) and thymol (3.48–9.45%). Glandular trichomes were analyzed using SEM (Scanning Electron Microscopy), as they are the sites of synthesis of free volatile compounds. We have detected glandular trichomes, consisting of a one stalk cell and two elliptically shaped head cells, and non-glandular (unbranched, bi-cellular to multicellular) trichomes on stems, leaves and the sepals. Data for volatile compounds from EOs and hydrosols were analyzed using Principal Component Analyses (PCA) to demonstrate variations in the composition of the volatile compounds identified. Isolated samples of EO and hydrosols were analyzed for their antioxidant activity using two methods, DPPH (2,2-diphenyl-1-picrylhydrazyl) and ORAC (Oxygen Radical Absorbance Capacity). The essential oils showed higher antioxidant activity than the hydrosols in ORAC method, but lower activity by the DPPH method. The isolates were also tested for their antiproliferative activity on different types of cancer cells and also on two lines of healthy cells, and the results showed that the extracts were not toxic to the cell lines tested. Total polyphenols, total tannins, total flavonoids and total phenolic acids were also analyzed and determined spectrophotometrically. The free volatile compounds of Veronica austriaca ssp. jacquinii can be considered as a safe natural product.

scholarly journals Chemical Compositions and Antioxidant Activities of Indonesian Citrus Essential Oils and Their Elucidation Using Principal Component Analysis

2020 ◽  
Author(s):  
Euis Julaeha ◽  
Kristin Shinta Dewi ◽  
Mohamad Nurzaman ◽  
Tatang Wahyudi ◽  
Tati Herlina ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
Antioxidant Activity ◽  
Essential Oils ◽  
Antioxidant Activities ◽  
Principal Component ◽  
Component Analysis ◽  
Chemical Compositions ◽  
Dpph Method ◽  
Ic50 Values ◽  
Citrus Essential Oils

Citrus essential oils (EOs) have various bioactivities like antioxidants, with many applications. Antioxidant activities depend on the chemical compositions of the EOs, which are affected by climate, soil, and geographical region. Thus, investigations on chemical compositions and antioxidant activities of Citrus EOs in different countries are valuable. In this study, we distilled EOs from peels of Indonesian-grown Citrus, including C. nobilis, C. limon, C. aurantifolia, C. amblycarpa, and Citrus spp.Chemical compositions of EOs were analyzed using Gas Chromatography-Mass Spectrometer (GC-MS), whereas the antioxidant activities were determined by employing 2,2-diphenyl-2-picrylhydrazyl (DPPH) method. Furthermore, principal component analysis (PCA) was applied to elucidate the main contributing compounds for antioxidant activity. The results show that all EOs possess unique chemical characteristics, with limonene as the majority constituent. For antioxidant activities, C. limon and C. amblycarpa EOs are the two strongest, IC50 values below 7.00 μL/mL. PCA approach suggests that -terpinene mainly contributes to the high antioxidant activities of C. limon and C. amblycarpa. Moreover, o-cymene, thymol, p-cymene, and α-pharnesene may also be responsible for the antioxidant activity of C. limon EO. These results are valuable information for the applications of Citrus EOs as antioxidant sources.

Comparative study of the simultaneous action of three essential oils on Aspergillus flavus and Sitophilus zeamais Motsch

Food Control ◽  
2010 ◽  
Vol 21 (2) ◽  
pp. 186-190 ◽  
Author(s):  
N.L. Tatsadjieu ◽  
A. Yaouba ◽  
E.N. Nukenine ◽  
M.B. Ngassoum ◽  
C.M.F. Mbofung
Keyword(s):  
Comparative Study ◽  
Essential Oils ◽  
Aspergillus Flavus ◽  
Sitophilus Zeamais ◽  
Simultaneous Action ◽  
Sitophilus Zeamais Motsch

scholarly journals Development of C-Arylcalix[4]resorcinarenes and C-Arylcalix[4]pyrogallolarenes as Antioxidant and UV-B Protector

2019 ◽  
Vol 19 (2) ◽  
pp. 273 ◽  
Author(s):  
Jumina Jumina ◽  
Dwi Siswanta ◽  
Abdul Karim Zulkarnain ◽  
Sugeng Triono ◽  
Priatmoko Priatmoko ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Essential Oils ◽  
Similar Condition ◽  
Benzoyl Chloride ◽  
Absolute Ethanol ◽  
Strong Activity ◽  
Activity Test ◽  
Dpph Method ◽  
Ic50 Values ◽  
High Yields

Indonesia is rich with essential oils such as anise and clove leave oils. In respect to explore the potential utilization of these resources, it has been conducted the transformation of p-hydroxybenzaldehyde and vanillin (4-hydroxy-3-methoxy benzaldehyde) respectively derived from anise oil and clove leaves oil to a series of C-arylcalix[4]resorcinarenes and C-arylcalix[4]pyrogallolarene macrocycles. Treatment of these aldehydes with resorcinol in the presence of HCl in absolute ethanol at reflux for 8 h afforded C-4-hydroxyphenylcalix[4]resorcinarene (3a) and C-4-hydroxy-3-methoxy phenyl-calix[4]resorcinarene (3b) in good yields. When the aldehydes were treated with pyrogallol under the similar condition, the products were C-4-hydroxyphenyl calix[4]pyrogallolarene (3c) and C-4-hydroxy-3-methoxyphenylcalix[4]pyrogallolarene (3d) which were also obtained in excellent yields. Treatment of these calix[4]resorcinarenes and calix[4]pyrogallolarenes with cinnamoyl chloride and benzoyl chloride in pyridine afforded the corresponding cinnamate esters and benzoate esters in high yields. The resulted C-arylcalix-[4]resorcinarenes and C-arylcalix[4]pyrogallol arenes were subjected to antioxidant activity test using DPPH method and showed strong activity with IC50 values of 15–80 μg/mL. In terms of the synthesized calix cinnamates, the compounds showed UV-B absorption with SPF values of 15–30 at a concentration of 25 ppm which demonstrate their potential to be applied as a UV-B protector. Furthermore, these compounds were also tested for their photostability on the UV-B region and the results showed that the compounds were still unstable under irradiation for 30 min.

scholarly journals Polyphenols Content and Antimicrobial, Antioxidant and Hemolytic Activities of Essential Oils from Four Selected Medicinal Plants Growing in Algeria

2020 ◽  
Vol 9 (2) ◽  
pp. 65-75
Author(s):  
Noureddine Halla ◽  
Kebir Boucherit ◽  
Bankaddour Zeragui ◽  
Abdelkader Djelti ◽  
Ziane Belkhedim ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Essential Oils ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Extreme Environment ◽  
Positive Control ◽  
Mentha Piperita ◽  
Antioxidant Power ◽  
Dpph Method

The Saharan and steppe spontaneous plants are very characteristic because of their particular adaptation to the desert and extreme environment. Some species have pharmacological properties that give them a medicinal interest. The aim of the present work was to determine the polyphenol contents of essential oils obtained from four endemic plants growing in Algeria (Pituranthos scoparius, Myrtus nivellei, Rosmarinus officinalis and Mentha piperita), and study its biological activity, including antimicrobial, antioxidant, and hemolytic. The antimicrobial activity was evaluated by the microdilution method against twelve strains. The antioxidant activity was carried out by two methods (DPPH radical scavenging and reducing power). However, the hemolytic effect has been evaluated against the red blood cells. P. scoparius and M. piperita showed yields of essential oils higher than 1%. All the strains showed sensitivity against the essential oils tested with the exception of the C. albicans treated by R. officinalis essential oils. The most sensitive strain was C. albicans treated by P. scoparius essential oils by MIC of 0.0781 mg/mL, it was the same plant that shows the highest polyphenol content (14.78 ± 0.72 g GAE/g DS). The antioxidant activity by the DPPH method was greater for all essential oils tested by IC50 ranging from 0.69 ± 0.07 (R. officinalis) to 30.67 ± 2.12 mg/mL (M. nivellei). The R. officinalis essential oils reported more antioxidant power than the positive control (ascorbic acid). In reducing iron, it was the R. officinalis essential oils which were found to be the most active with an EC50 concentration of 9.67 ± 1.36 mg/mL. After 120 min incubation, minimal haemolysis (10%) was obtained with essential oils of R. officinalis at a concentration of 0.39 mg/mL. We conclude that P. scoparius essential oils showed the high content of polyphenols and R. officinalis essential oils reported more antioxidant power than the positive control (ascorbic acid).

scholarly journals Aktivitas Antioksidan dan Antimikroba Minyak Atsiri Kembang Leson

2020 ◽  
Vol 13 (2) ◽  
pp. 139-146
Author(s):  
Ambar Pratiwi ◽  
Inas Salimah
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Inhibitory Concentration ◽  
Herbal Medicines ◽  
Diffusion Method ◽  
E Coli ◽  
Ic50 Value ◽  
Dpph Method

AbstrakKembang leson adalah racikan jamu untuk mandi yang terdiri dari berbagai obat-obatan herbal yang umum ditemukan di Jawa. Kembang leson mengandung minyak atsiri utama, yaitu camphene 1,29%, benzene methyl cymene 4,93%, camphor 4,75%, cyclohexane methanol 7,56%, dan curdione 4,83%. Golongan senyawa minyak atsiri dapat menghambat radikal bebas 2,2-diphenyl-1-picrylhydrazyl (DPPH) dan memiliki aktivitas antibakteri yang ditunjukkan dengan uji aktivitas antibakteri terhadap Staphylococcus aureus dan Escherichia coli. Tujuan penelitian ini adalah untuk mengetahui aktivitas antioksidan dan antibakteri dari minyak atsiri kembang leson. Minyak Atsiri kembang leson diekstraksi dengan metode destilasi. Minyak atsiri yang diperoleh diuji aktivitas antioksidan dengan metode DPPH, serta aktivitas antibakteri dengan metode difusi. Hasil penelitian menunjukkan bahwa minyak atsiri kembang leson memiliki aktivitas antioksidan dengan persentase penghambatan DPPH sebesar 56,16%, dan memiliki nilai IC50 (half maximal inhibitory concentration) sebesar 825,78 ppm. Aktivitas antibakteri minyak atsiri kembang leson juga efektif menghambat pertumbuhan E. coli, tetapi belum mampu menghambat pertumbuhan S. aureus. Minyak atsiri kembang leson  dapat digunakan sebagai sumber antioksidan alami. Abstract Kembang leson is an herbal concoction used for bathing. It comprises various herbal medicines found in Java. Kembang leson contains essential oils such as camphene 1.29%, benzene methyl cymene 4.93%, camphor 4.75%, cyclohexane methanol 7.56% and curdione 4.83%. It is known that essential oils can reduce DPPH free radicals and have antibacterial activity against S. aureus and E. coli.  Our research to determine the antioxidant and antibacterial activity of kembang leson essential oil. The essential oil was extracted by distillation. The essential oil obtained was tested for antioxidant activity using the DPPH method, and antibacterial activity using the diffusion method. The results show that kembang leson essential oils have antioxidant and antibacterial activity. The percentage of DPPH inhibition was 56.16% and the IC50 value was 825.78 ppm. Antibacterial activity of kembang leson essential oil inhibited the growth of E. coli but could not inhibit the growth of S. aureus. Thus, kembang leson essential oils have antioxidant and antibacterial activity against E. coli, but only have antioxidant activity against S. aureus. Further studies are needed to determent the main ingredients that play an important role in the mechanism of antioxidants and antibacterial of kembang leson essential oil.

scholarly journals ANTIOXIDANT ACTIVITY, SKIN IRRITAION POTENTIAL AND CHEMICAL COMPOSITION OF CLOVE LEAF OIL FROM WEST JAVA INDONESIA

2021 ◽  
Vol 23 (1) ◽  
pp. 24
Author(s):  
Dwinna Rahmi ◽  
Retno Yunilawati ◽  
Ira Setiawati ◽  
Irwinanita Irwinanita ◽  
Bumiarto Nugroho Jati ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Skin Irritation ◽  
Chemical Compositions ◽  
Clove Oil ◽  
West Java ◽  
Specific Chemical ◽  
Dpph Method ◽  
Leaf Oil

ANTIOXIDANT ACTIVITY, SKIN IRRITATON POTENTIAL, AND CHEMICAL COMPOSITION OF CLOVE LEAF OIL FROM WEST JAVA INDONESIA. Essential oils with specific chemical compositions have the potential as a source of antioxidants in cosmetics, however, in general, essential oils are not safe because of the potential for skin irritation. This study aims is to perform fractionation to obtain clove oil with a chemical composition that is safe on the skin and does not reduce its antioxidant activity. Fractionation was performed at a temperature of 100 – 285 oC. Chemical composition was determined by Mass Chromatography Spectrometry (GC-MS), antioxidant activity by DPPH method, and irritation potential by in-vivo patch test. The results showed chemical components of clove oil were eugenol, copaene, beta caryophyllene, iso-eugenol, alpha caryophyllene, cadinene, caryophyllene oxide, caryophylla and beta caryophylla. Fractions with chemical compositions of eugenol and beta caryophyllene as the main components and copaene, iso-eugenol, alpha caryophyllene, cadinene as secondary components have very light erythema and no potential irritation to the skin. The fraction produced from the fractionation of crude clove oil at a temperature of 235 – 260 oC has the highest antioxidant activity of 10.17 mg/L.

scholarly journals Essential Oils as Natural Antimicrobial and Antioxidant Products in the Agrifood Indus

2020 ◽  
pp. 55-69 ◽  
Author(s):  
M. Dolores Ibáñez ◽  
M. Pilar López-Gresa ◽  
Purificación Lisón ◽  
Ismael Rodrigo ◽  
José María Bellés ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Pseudomonas Syringae ◽  
Antioxidant Activities ◽  
Phytopathogenic Fungus ◽  
Oregano Essential Oil ◽  
Dpph Method ◽  
Main Component ◽  
Disk Method

Consumers are aware of the dangers arising from the use of synthetic antioxidants and antimicrobials in the agrifood industry, demanding safer and “greener” alternatives. In this study, the antioxidant activity of commercial essential oils through DPPH method, their antimicrobial effects against the bacterium Pseudomonas syringae and the phytopathogenic fungus Fusarium oxysporum by means of the standardized disk method were determined. Clove along with winter savory, cinnamon and oregano essential oils as well as carvacrol showed the highest antioxidant activity comparable to reference standards. Wintergreen essential oil was the most potent inhibitor against P. syringae growth at the highest doses (20 and 10 μL). Oregano essential oil and its main component carvacrol were able to stop the bacterium growth even at the lowest treatment (1 μL). Cinnamon, oregano and peppermint essential oils inhibited F. oxysporum development at all doses (20, 10 and 5 μL) assayed. In general, most of the essential oils displayed more antifungal than antibacterial and antioxidant activities.

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