Chemical constituents of the stem bark of Acacia AuriculiformisA.Cunn ex. Benth. (fabaceae)

2020 ◽  
Vol 23 ◽  
pp. 88-91
Author(s):  
A.A. Ahmadu ◽  
B.A. Lawal ◽  
B. Olanipekun ◽  
A. Udobre ◽  
N. Tsafantakis ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Secondary Metabolites ◽  
Acid Ester ◽  
Spectroscopic Analysis ◽  
Chemical Constituents ◽  
Stem Bark ◽  
Acacia Auriculiformis ◽  
Compound I ◽  
Literature Report ◽  
First Time

The genus Acacia has been known to be a rich source of many secondary metabolites. This study was carried to isolate chemical constituents present in the stem bark of Acacia auriculiformis. The dichloromethane extract of the stem bark of Acacia auriculiformis was obtained by maceration. The extract obtained was subjected to silica gel column chromatography and preparative TLC. The isolated compounds were identified by spectroscopic analysis. This led to the isolation of ferulic acid ester (I), along with a steroid (II) and a triterpenoid (III). The structure of compound I was established using spectroscopic analysis (UV, IR, NMR and mass spectrometry) and identified to be dodecyl-4-hydroxy-3-methoxy-trans-cinnamate (I), compounds II and III were found to be á-spinasterol and lupenol respectively, based on the comparison of their spectral data NMR and MS with literature report. Compound I is being reported for the first time in the genus Acacia.

scholarly journals Chemical constituents of Euphorbia tirucalli L.

2019 ◽  
Vol 2 (5) ◽  
pp. 76-82
Author(s):  
Dung Thi Kim Le ◽  
Hao Xuan Bui ◽  
Tuyet Thi Anh Nguyen ◽  
Tuyen Nguyen Kim Pham ◽  
Huy Thuc Duong
Keyword(s):  
Thin Layer ◽  
Gallic Acid ◽  
Spectroscopic Analysis ◽  
Chemical Constituents ◽  
Normal Phase ◽  
Chemical Structures ◽  
Euphorbia Tirucalli ◽  
Arjunolic Acid ◽  
First Time ◽  
Layer Chromatography

Euphorbia tirucalli has not been chemically studied much in Vietnam. This research described the isolation and elucidation of compounds isolated from the plant collected in Binh Thuan. Multiple chromatographic methods were applied, including normal phase silica gel column chromatography and thin-layer chromatography. Seven compounds were isolated and their chemical structures were elucidated by spectroscopic analysis as well as comparing their data with the ones in the literature. They are arjunolic acid (1), eriodictyol (2), quercitrin (3), afzelin (4), scopoletin (5), 3,3′,4- trimethylellagic acid (6), and gallic acid (7). Among them, compound 1 a major component was isolated for the first time in Euphorbia genus, while three compounds 2, 4, and 5 were isolated from this species for the first time.

scholarly journals Analysis of Major Chemical Constituents in Luan-Pao-Prescription Using Liquid Chromatography Coupled with Electrospray Ionization Mass Spectrometry

2008 ◽  
Vol 3 (5) ◽  
pp. 1934578X0800300
Author(s):  
Jin-qiang Zhang ◽  
Min Yang ◽  
Bao-hong Jiang ◽  
Hui-lian Huang ◽  
Guang-tong Chen ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Electrospray Ionization ◽  
Chemical Constituents ◽  
Negative Ion ◽  
Female Sterility ◽  
Ionization Mass ◽  
Chinese Herbal ◽  
Major Chemical ◽  
First Time

Luan-Pao-Prescription is a famous Chinese herbal formula, which is commonly used for the treatment of female sterility in clinical practice in China. In the present paper, a reliable method based on liquid chromatography coupled with electrospray ionization tandem mass spectrometry in both positive and negative ion modes has been established for the analysis of major chemical constituents in Luan-Pao-Prescription. A total of 34 compounds were either identified or tentatively characterized. These compounds include flavonoids, anthraquinones, iridoids, xanthones and organic acids. Flavonoids were the major constituents of the formula. The results profiled the chemical composition of Luan-Pao-Prescription comprehensively for the first time.

scholarly journals A New Bianthracene C-arabinopyranoside from Senna septemtrionalis

2010 ◽  
Vol 5 (5) ◽  
pp. 1934578X1000500
Author(s):  
Gizachew Alemayehu ◽  
Legesse Adane ◽  
Berhanu M. Abegaz
Keyword(s):  
Secondary Metabolites ◽  
Nmr Spectra ◽  
Spectroscopic Analysis ◽  
Stem Bark ◽  
The Novel

Chrysophanol, physcion, emodin, floribundone-1, 5,7′-physcion-fallacinol, and the novel 5,7′-physcion-physcion-10′- C-α-arabinopyranoside were isolated from the stem bark of Senna septemtrionalis. The structures of these secondary metabolites were determined on the basis of spectroscopic analysis, especially from NMR spectra in conjunction with COSY, HMQC, HMBC and TOCSY.

scholarly journals Chemical Constituents and Protection of Biodiversity of Corypha taliera Roxb., a Critically Endangered Plant of Bangladesh

2018 ◽  
Vol 20 (2) ◽  
pp. 213-220
Author(s):  
Akhtaruzzaman Chowdhury ◽  
Md Ashraful Alam ◽  
Md Shafiullah Shajib ◽  
Mohammad Abdullah Al Mansur ◽  
Mohammad A Rashid
Keyword(s):  
Spectroscopic Analysis ◽  
Methanol Extract ◽  
Chemical Constituents ◽  
Pharmaceutical Journal ◽  
Critically Endangered ◽  
Ex Situ ◽  
Endangered Plant ◽  
Separation And Purification ◽  
First Time ◽  
Mature Seeds

This article focuses on the chemical constituents and protection of biodiversity through plantation of saplings of Corypha taliera Roxb., a critically endangered plant of Bangladesh. Until 2010, the tree in the campus of University of Dhaka, used to be considered as the lone surviving species in the world in nature. Succesive chromatographic separation and purification of the methanol extract of air dried flowers of C. taliera provided β-sitosterol (1), β-amyrin (2), and betulinic acid (3) for the first time from its flowers. The structures of these purified compounds were established by extensive spectroscopic analysis and comparison of spectral data with published values as well as co-TLC with authentic samples. On the other hand, 500 mature seeds were sown in seed beds in the Medicinal Plant Garden of Faculty of Pharmacy, University of Dhaka, and Azimpur Government Officers' Quarter premises. After 40 days, the root was first seen to grow in its habitat and 85 days later the shoot developed up to 2.5 cm in height. The rate of germination was found to be 89-93%. The produced saplings were later on planted in different places of Bangladesh for conservation of the plant and protection of biodiversity by ex situ arrangement.Bangladesh Pharmaceutical Journal 20(2): 213-220, 2017

scholarly journals Chemical constituents of the lichen Roccella sinensis growing in Binh Thuan province

2019 ◽  
Vol 2 (5) ◽  
pp. 63-67
Author(s):  
Huy Thuc Duong ◽  
Hao Xuan Bui
Keyword(s):  
Spectroscopic Analysis ◽  
Chemical Constituents ◽  
Human Lung Cancer ◽  
Cytotoxic Activities ◽  
Human Breast ◽  
Nmr Data ◽  
Liver Hepatocellular Carcinoma ◽  
First Time ◽  
Layer Chromatography ◽  
Mcf 7

The lichen Roccella sinensis has not been studied chemically. This research described the isolation and elucidation of compounds isolated from the lichen Roccella sinensis collected in Binh Thuan. Phytochemistry investigation of this lichen was carried out by using normal phase silica gel column chromatography and thin-layer chromatography. Six compounds was isolated. Their structures were established by extensively spectroscopic analysis as well as comparison with NMR data in the literatures. They are (+)-D-montagnetol (1), (+)-D-erythrin (2), lecanorin (3), 1-acetylerythritol (4), (E)-nostodione A (5), and 2,4-dihydroxyphthalide (6). This is the first time compounds 3 6 were found in the Roccella genus. Compounds 1, 2, and 6 were evaluated for their cytotoxic activities against HepG2 (liver hepatocellular carcinoma), NCI-H460 (human lung cancer), MCF-7 (human breast cancer), and HeLa (human epithelial cancer) and all of them showed no activity.

scholarly journals Triterpenoid and Fatty Acid Contents from the Stem Bark of Cordia dichotoma (Forst f.)

Folia Medica ◽  
2018 ◽  
Vol 60 (4) ◽  
pp. 594-600 ◽  
Author(s):  
Pankajkumar B. Nariya ◽  
Vinay J. Shukla ◽  
R. N. Acharya ◽  
Mukeshkumar B. Nariya ◽  
Jayesh M. Dhalani ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Chemical Constituents ◽  
Methyl Esters ◽  
Petroleum Ether Extract ◽  
Folk Medicine ◽  
Chemical Compounds ◽  
Stem Bark ◽  
Gas Chromatography Mass Spectrometry ◽  
Medicinal Purpose ◽  
First Time

Abstract Aim: To isolate and determine the chemical constituents of the stem bark of Cordia dichotoma (Forst f.), a plant used for medicinal purpose in folk medicine. Materials and methods: Petroleum ether extract of the stem bark was used for this study. Saponification process was performed to separate fatty acid and unsaponifiable matter. Results: One triterpenoids, α-amyrin was isolated from the bark by using isocratic elution. The chemical compounds isolated, for the first time, were analyzed by GC/MS, IR, and UV. The chemical composition of the fatty acids methyl esters (FAMEs) in bark of Cordia dichotoma were also analyzed by gas chromatography-mass spectrometry. After methyl-esterification, 17 components were identified in the bark. The derivatization conditions were investigated in order to validate this method. Conclusion: The present analysis revealed that Cordia dichotoma stem bark contains 17 fatty acid. The principal themes of the review highlight the development and application of chromatographic techniques for the separation, isolation and detection of the compounds.

scholarly journals Essential Oil and Major Non-Volatile Secondary Metabolites from the Leaves of Amazonian Piper subscutatum

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1168
Author(s):  
Jorge Ramírez ◽  
María Daniela Andrade ◽  
Giovanni Vidari ◽  
Gianluca Gilardoni
Keyword(s):  
Mass Spectrometry ◽  
Nmr Spectroscopy ◽  
Secondary Metabolites ◽  
Essential Oil ◽  
Volatile Fraction ◽  
Organic Fraction ◽  
X Ray ◽  
X Ray Crystallography ◽  
Ecuadorian Amazon ◽  
First Time

The essential oil and the major non-volatile secondary metabolites from the leaves of Piper subscutatum (Miq.) C. DC. (Family Piperaceae), collected in the Ecuadorian Amazon, were analyzed for the first time in the present study. The essential oil was submitted to chemical and enantioselective analyses by GC-MS and GC-FID. (E)-β-caryophyllene (25.3–25.2%), β-chamigrene (10.3–7.8%), (E)-nerolidol (8.1–7.7%), β-selinene (7.2–7.7%), δ-cadinene (2.7–3.9%), bicyclogermacrene (3.7–2.4%), and β-pinene (2.6–3.4%) were the major components. The enantioselective analysis, carried out on a β-cyclodextrin-based column, showed four scalemic mixtures in which (1R,5R)-(+)-α-pinene, (1S,5S)-(−)-β-pinene, (S)-(−)-limonene, and (1R,2S,6S,7S,8S)-(−)-α-copaene were the major enantiomers, with enantiomeric excesses of 28.8%, 77.8%, 18.4%, and 6.0%, respectively. The study was complemented with the chemical analysis of the organic fraction dissolved in the hydrolate, whose major components were 6-methyl-5-hepten-2-one (63.7–64.4%) and linalool (6.5–6.0%). Concerning the non-volatile fraction, five lignans were the major components. (–)-Beilshminol B, (–)-grandisin, (–)-3′,4′-methylenedioxy-3,4,5-trimethoxy-7,7′-epoxylignan, (–)-3′,4′-methylenedioxy-3,4,5,5′-tetramethoxy-7,7′-epoxylignan, and (–)-3,4,3′,4′-dimethylenedioxy-5,5′-dimethoxy-7,7′-epoxylignan were identified by means of NMR spectroscopy, mass spectrometry and X-ray crystallography. The absolute configuration 7S,8S,7′S,8′S was tentatively assigned to all of them.

scholarly journals Isolation of 2, 5-dithia-3, 6-diazabicyclo [2.2.1] heptane and GC-MS analysis of silylated extract from Tragia benthamii

2020 ◽  
Vol 22 (2) ◽  
pp. 075-080
Author(s):  
O.S. Balogun ◽  
I.A. Oladosu ◽  
Zhiqiang Liu
Keyword(s):  
Secondary Metabolites ◽  
Aqueous Ethanol ◽  
Chemical Constituents ◽  
Dry Weight ◽  
Spectroscopic Techniques ◽  
Chromatographic Purification ◽  
Pure Compound ◽  
Ms Analysis ◽  
First Time ◽  
Derivatives Of

Tragia benthamii is a medicinal plant of repute endemic in West Africa. However, despite the traditional uses of the plant its secondary metabolites are yet to be profiled. Thus, this study aimed at identifying the chemical  constituents of T. benthamii. Air-dried samples (600 g) of the plant were extracted with aqueous ethanol (95 %) and thereafter, portions of the crude extract obtained were used separately for chromatographic  purifications, GC-MS analysis and spectrophotometric quantitation of the secondary metabolites. The chromatographic purification of the extract afforded a pure compound elucidated as 2,5-dithia-3,6-diazabicyclo [2.2.1] heptane using spectroscopic techniques. Upon silylation and GC-MS analysis of the extract, derivatives of sugar (34.65%), fatty acids (24.11%) and phytol (21.00%) were identified as principal constituents. Also, a total of 6.25 mg rutin/g, 5.18 mg ginsenoside/g and 6.84 mg glucose/g dry weight sample were obtained for flavonoids, saponins and carbohydrate, respectively from the spectrophotometric quantitation. These secondary metabolites are being reported for the first time from this plant. Keywords: Tragia benthamii; secondary metabolites; quantitative analysis; silylation

scholarly journals Chemical constituents of leaves Dialium cochinchinense Pierre

2021 ◽  
Vol 4 (3) ◽  
pp. 1131-1136
Author(s):  
Vu Thi Huyen ◽  
Doan Thi Thuy Ai ◽  
Nguyen Thi Hien
Keyword(s):  
Secondary Metabolites ◽  
Nmr Spectra ◽  
Spectroscopic Analysis ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Antimicrobial Activities ◽  
Tropical Regions ◽  
First Report ◽  
Isolation And Characterization

The genus Dialium belongs to the Caesalpinioideae family, consisting of approximately 30 species distributed in the tropical regions. Secondary metabolites from the  Dialium genus have been reported to exhibit various biological activities including antioxidant, cytotoxicity and antimicrobial activities. This work describes the isolation and characterization of five compounds from the leaves of Dialium cochinchinense Pierre. Their structures were established by spectroscopic analysis, including MS and NMR spectra. Accordingly, the isolated compounds were identified to be lupeone (1), b-sitostenone (2), β-sitosterol (3), daucosterol (4), and dihydrokaempferide (5). To the best of our knowledge, this is the first report of the isolation of compounds 1 and 5 from the genus Dialium.

scholarly journals HIGH RESOLUTION LC-MS/MS SCREENING FOR SECONDARY METABOLITES IN BULGARIAN SPECIES OF GENUS Astragalus L.

Química Nova ◽  
2021 ◽  
Author(s):  
Aleksandar Shkondrov ◽  
Ilina Krasteva
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
High Resolution ◽  
Secondary Metabolites ◽  
High Performance ◽  
Phenological Stages ◽  
Phytochemical Content ◽  
Ionisation Mass Spectrometry ◽  
First Time ◽  
Ionisation Mass

The phytochemical content of some Astragalus species distributed in Bulgarian flora has been previously studied. Among other compounds, flavoalkaloids, acylated flavonoids, flavonoid triglycosides and cycloartane saponins have been isolated so far. The composition of the rest of the representatives of this genus in Bulgaria is not explored yet. The aim of this study was to perform a screening for the presence of selected rare secondary metabolites (flavoalkaloids, acylated flavonoids, flavonoid triglycosides and cycloartane saponins) in selected Astragalus species. Samples were collected in different phenological stages and from different locations in the country. A novel and rapid ultra-high performance liquid chromatography – high resolution electrospray ionisation mass spectrometry (UHPLC-HRESIMS) method was developed and applied. For the first time a flavoalkaloid glycoside was determined from extracts of A. onobrychis var. chlorocarpus and A. glycyphylloides. From A. depressus an acylated derivative of kaempferol was newly identified. The flavonol triglycosides camelliaside A, alcesefoliside and mauritianin were proved in samples of A. glycyphylloides, A. onobrychis var. chlorocarpus and A. cicer for the first time as well.

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