Haematological and histopathological examinations of African mud catfish (Clarias gariepinus) exposed to petroleum wastewater

2021 ◽  
Vol 20 (2) ◽  
pp. 127-144
Author(s):  
R.Y. Oladunjoye ◽  
O.O. Fafioye ◽  
R.A. Asiru ◽  
G.O. Bakare ◽  
A.A. Odusolu
Keyword(s):  
Tap Water ◽  
Clarias Gariepinus ◽  
Control Fish ◽  
Exposed Fish ◽  
Petroleum Refinery Wastewater ◽  
Commercial Feed ◽  
Significant Difference ◽  
Fish Samples ◽  
Visible Lesion ◽  
Growth Changes

Effects of petroleum refinery wastewater on Clarias gariepinus juvenile were investigated. Commercially obtained C. gariepinus fingerlings were acclimatized in a plastic tank (100 L capacity) of de-chlorinated tap water at 25±2°C for 14 days and fed with commercial feed pellet at 2% body weight of the fingerlings. Bioassay tests were carried out in four transparent plastics tank with nominal concentrations of 100 ml, 200 ml, 300 ml of the wastewater added to 40L of de-chlorinated tap water and only de-chlorinated tap water as control. Each tank contains twenty fish samples, while the assay was replicated three times concurrently. Following standard procedures, behavioural response, growth changes, haematological and histopathological tests were carried out on the samples. Significant reduction in the weight was observed in the fingerlings cultured with the wastewaters, while no significant difference occurred in the control fish. Highest values of Packed Cell Volume (PCV) (22), Haemoglobin (HB) (7.0), Red Blood Cell (RBC) (1.62) and endocochlear potential (EP) (5) were recorded for the control fish than exposed fish. On the other hand, Haptoglobin (HP), Mean Corpuscular Volume (MCV) and Mean Corpuscular Haemoglobin (MCH) were higher in the blood of exposed fish than in control. Histopathologically, exposed fishes showed no visible lesion in gills except from the thickening of the lamellae as the concentration of wastewater increases, indicating an increase in tissue disintegration. Similarly, gross tissue disintegration was observed in those fish exposed to 200ml wastewater as evidenced by the presence of large open spaces (hepatocytes) in the liver.

Impact of Ogun River pollution on antioxidants and proximate composition of African catfish (Clarias gariepinus)

2019 ◽  
Vol 46 (3) ◽  
pp. 163-184
Author(s):  
B. O. Onunkwor ◽  
T. O. Akinpelu ◽  
O. J. Oladipupo ◽  
R. N. Ugbaja ◽  
O. J. Olaoye ◽  
...  
Keyword(s):  
Proximate Composition ◽  
Clarias Gariepinus ◽  
Chemical Properties ◽  
Oxygen Demand ◽  
Control Fish ◽  
Average Weight ◽  
African Catfish ◽  
Fish Samples ◽  
The Impact ◽  
Ogun River

Most rivers in Nigeria are polluted without conscious efforts to abate ensuant consequences. Thus, Clarias gariepinus, an aquatic commercial food from Ogun River, Nigeria, was studied to investigate the impact of pollution on antioxidants and proximate composition. During the rainy season, 50 C. gariepinus (average weight 426.53 ± 24.40g and length 37.73 ± 0.95cm) were site sampled, 10 from each of Ago-Odo, Ago-Ika, Oke-Sokori, and Quarry along Ogun River course. Ajegunle-Adao cultured pond, served as control. The parameters assayed for included: some heavy metals and physico-chemical properties of sampled water, antioxidants and proximate composition of the fish samples. Physicochemical parameters of river studied showed that temperature of all sites ranged from 26 to 29°C, pH: 6.9 to 7.8, dissolved oxygen: 9.5 to 21.6, biochemical oxygen demand: 3.60 to 14.10, and chemical oxygen demand: 60 to 650. Lead (Pb) concentration ranged from 4.0 to 17.0 μg/L and cadmium (Cd): 0.0 to 6.0 μg/L. Blood Pb, Cd, activities of: superoxide dismutase, catalase, glutathione-S-transferase, plasma xanthine oxidase, and lipid peroxidation level of fish samples from Ago-Ika were most significantly (p < 0.05) high, with significant (p < 0.05) lowest reduced glutathione concentration amongst the samples from polluted sites when compared to control fish. Proximate analysis showed that the fish samples from Ogun River had significantly (p < 0.05) lower protein, fat contents, and higher nitrogen-free extract while Ago-Ika, Oke-Sokori and Quarry sites had significantly (p < 0.05) lower moisture, ash and fibre contents when compared with control. It can be concluded that the impact of pollution induced oxidative stress and significantly diminished the nutritional value of C. gariepinus from Ogun River.

scholarly journals Cytogenotoxicity of Abattoir Effluent in Clarias gariepinus (Burchell, 1822) Using Micronucleus Test

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Chibuisi G. Alimba ◽  
Ebenezer O. Ajayi ◽  
Titilayo Hassan ◽  
Akindayo A. Sowunmi ◽  
Adekunle A. Bakare
Keyword(s):  
Micronucleus Test ◽  
Tap Water ◽  
Clarias Gariepinus ◽  
Oxygen Demand ◽  
Negative Control ◽  
Control Fish ◽  
Dependent Manner ◽  
Nuclear Abnormality ◽  
And Control ◽  
Positive Controls

The cytogenotoxic potential of abattoir effluent from Bodija, Nigeria, was investigated using micronucleus test in Clarias gariepinus. Fish was exposed to five different concentrations: 0.2, 0.4, 0.8, 1.6, and 3.1% of the effluent for 7, 14, and 28 days. Tap water and 0.02 mL/L of benzene were used as negative and positive controls, respectively. Physicochemical parameters and heavy metals were analyzed in the effluent in accordance with standard methods. After exposure, blood was collected from the treated and control fish and slides were prepared for micronuclei (MN) and nuclear abnormality evaluation in the peripheral erythrocytes. The effluent induced significant (p<0.05) increase in the frequency of MN in a time dependent manner. Similarly, the frequency of total nuclear abnormalities (blebbing, notch, bud, binucleation, and vacuolation) was higher in the exposed fish than the negative control. Electrical conductivity, nitrate, biochemical oxygen demand, chemical oxygen demand, arsenic, and copper analyzed in the effluent may have provoked the observed cytogenetic damage. The findings herein suggest the presence of clastogens and cytotoxins in Bodija abattoir wastewater which are capable of increasing genomic instability in aquatic biota.

scholarly journals Polycyclic Aromatic Hydrocarbons (PAH) in Fingerlings of Clarias gariepinus (Burchell, 1822) to Petroleum

2018 ◽  
Author(s):  
Christopher Onyemaechi Ezike ◽  
Felix Okaliwe Echor
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Crude Oil ◽  
Aromatic Hydrocarbons ◽  
Oil Spills ◽  
Clarias Gariepinus ◽  
Diesel Oil ◽  
Exposed Fish ◽  
Significant Difference ◽  
Polycyclic Aromatic ◽  
Experimental Treatments

One hundred and twenty (120) fingerlings of Clarias gariepinus (mean weight: 0.96 &plusmn; 0.1g) were randomly exposed to 4 experimental treatments of petroleum, based on LC50 values (6.4mg/L of crude oil, 8.7mg/L of petrol, 8.0mg/L of kerosene and 7.8mg/L of diesel oil) and replicated thrice, to determine polycyclic aromatic hydrocarbons (PAH) in exposed fish for 96 h. There was no significant difference (P &gt; 0.05) in total (PAHs) between crude oil (97.1 ng/uL) and diesel (97.2 ng/uL) exposed fish and also between petrol (53.2 ng/uL) and kerosene (49.6 ng/uL) exposed fish, but there was a significant difference (P &lt; 0.05) in PAH levels of the crude oil/diesel exposed -groups of fish compared to petrol/kerosene exposed -groups of fish (97.1/97.2 and 53.2/49.6 ng/uL). Naphthalene correlated positively to benzo a anthracene (r=0.672, (P &lt; 0.05), benzo b fluoranthene (r=0.681, P &lt; 0.05) and chrysene (r=0.615, P &lt; 0.05) but did not correlate to fluorene. Benzo a anthracene correlated positively to benzo a pyrene (r=0.578, P &lt; 0.05), phenathrene (r=0.685, P &lt; 0.05) but did not correlate to acenaphthene. Fluorene correlated positively to benzo a pyrene (r=0.695, P &lt; 0.05) but did not correlate to chrysene. Chrysene correlated positively to dibenzo a,h, pyrene (r=0.658, P &lt; 0.05) to phenathrene and benzo b fluoranthene (r=0.659, P&lt; 0.05). Indeno 123 cd- pyrene and fluranthene however did not correlate to other PAHs except naphthanene, acenaphthene and acenaphthylene. The level of PAH in fish may translate to the toxicity effect since crude oil and diesel with lower LC50 (6.4 and 7.8 mg/L)&nbsp;&nbsp; deposited greater PAH than kerosene and petrol with higher LC50 (8.7 and 8.0 mg/L) in fingerlings of C. gariepinus. High risk to cancer disorders may occur in exposed fish to petroleum with high incidence of fluorene , anthracene, pyrene and benz a anthracene which correlated positively to benzo a pyrene which provide some basis for predicting impact of oil spills on fingerling population.

scholarly journals Growth performance of Clarias gariepinus fed varying dietary phosphorus levels

2017 ◽  
Vol 4 (3) ◽  
pp. 215-220
Author(s):  
Ezike Christopher Onyemaechi ◽  
Chukwuka Doris Queen ◽  
Echor Felix Okaliwe
Keyword(s):  
Growth Performance ◽  
Control Diet ◽  
Clarias Gariepinus ◽  
Palm Kernel ◽  
Fish Growth ◽  
Control Fish ◽  
African Catfish ◽  
Dietary Phosphorus ◽  
Significant Difference ◽  
And Control

One hundred and twenty (120) juveniles of African catfish Clarias gariepinus were obtained in May 2015 from a reputable Fish Farm in Enugu to the University Research Farm (latitude 074o North and 082o South, longitude 068o East and 076o West with annual mean temperature of 30oC). The fish were fed for 49 days from June – July 2015 with diets containing 4 different inclusion levels of phosphorus T1 [0.6% P], T2 [0.8% P], T3 [1.00% P], T4 [1.2% P] and T5 [0% P] which served as control diet, to determine optimum P requirement for fish growth at probability level of P < 0.05 between various treatments and control. Each diet was formulated to contain 40% crude protein composed of yellow maize, soybean cake, palm kernel cake and fish meal. Fish fed with graded levels of phosphorus were significantly higher (P < 0.05) than control in FW and MDWG. There was however no significant difference (P > 0.05) in FCR and PER between T1, T2, T4 and control but T3 was significantly (P < 0.05) better in FCR (0.33) than control (0.39). Similarly, T3 was significantly (P < 0.05) higher in PER than control. Fish in T3 had the best growth performance indicative that 1% P is the optimum requirement for the growth of C. gariepinus.Res. Agric. Livest. Fish.4(3): 215-220, December 2017

scholarly journals Performance of Different Biomaterials as Carbon Sources on the Immunological Response and Oxidative Status of African Catfish Clarias gariepinus in Biofloc Systems

2021 ◽  
Vol 22 (2) ◽  
Author(s):  
Omoniyi Michael Popoola ◽  
Oyelade Ayomide Miracle
Keyword(s):  
Clarias Gariepinus ◽  
Carbon Sources ◽  
Immunological Response ◽  
Oxidative Status ◽  
African Catfish ◽  
Water Parameters ◽  
Enzymes Activity ◽  
Commercial Feed ◽  
Significant Difference ◽  
And Control

A trial was performed to investigate how carbon sources can affect the welfare status of African catfish (3.8±0.2g and 8±0.11 cm) juveniles in biofloc systems. Inocula was prepared in glass tanks (5L) by adding 20g of pond bottom soil in well aerated water (1L) containing 10mg L-1 ammonium sulphate (NH)4SO4 and 400mg L-1 of four different carbon sources (tapioca, wheat offal, brewery and cassava peel) for 24 hours. Each treatment group (carbon sources and control) were replicated and each tank contained 1000 Clarias gariepinus juvenile, fed with commercial feed (Crude protein 42%) at 5% of their body weight 72 days. The water quality showed that all water parameters remained at concentrations suitable for Clarias gariepinus culture in the studied systems. The enzymes activities were noticed to be different across the biofloc and the selected organs. There were significant differences in serological content in fish between the treatment’s groups (P<0.05). The significant difference was found between the treatments in case of enzymes activity (P<0.05). The study shows that the welfare status with reference to digestive enzymes activity, oxidative status and extent of the immune system stimulation in BFT system is carbon source dependent.

scholarly journals Hematological, Biochemical and Biometric Changes in Clarias Gariepinus Exposed to Antipsychotic Drug Chlorpromazine

2021 ◽  
Author(s):  
Martins Nnamdi Okpe ◽  
Christabel Chukwuebuka Eze ◽  
Hope Chinwe Ezinwa ◽  
Uduak Aletan ◽  
Henrietta Ijeoma Kelle ◽  
...  
Keyword(s):  
Blood Cells ◽  
Biochemical Analysis ◽  
Condition Factor ◽  
Clarias Gariepinus ◽  
White Blood Cells ◽  
Hepatosomatic Index ◽  
Exposed Fish ◽  
Toxicological Effect ◽  
Significant Difference ◽  
Utmost Care

Abstract Chlorpromazine (CPZ) is a neuroleptic and antipsychotic medication for individuals suffering from schizophrenia and other medical conditions. This study investigated the effects of CPZ on the hematological, biochemical, and biometric characteristics in juvenile Clarias gariepinus. The fish were exposed to 0.53, 1.06 and 2.11 mgl−1 CPZ for 15 days after which they were withdrawn from the toxicant and allowed to recover for 5 days. Blood were sampled from the fish on day 1, 5, 10, 15 and during the 5-day recovering for hematological and biochemical analysis and thereafter, the fish were sacrificed for the morphometric analysis. While the values of the white blood cells significantly increased in the exposed fish, the hemoglobin, erythrocytes and packed cell volume decreased. Compared with the control, there were no significant differences in the values of the blood derivatives in the exposed fish. The values of protein and glucose reduced but that of aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase were significantly elevated. Though there was no significant difference in the condition factor, a significant increase in hepatosomatic index occurred on day-15 at 5.28 mg/L CPZ. After the 5-day withdrawal from the drug, most of the studied parameters returned to the control values. The present study indicated that CPZ is toxic to fish and should be used with utmost care to guard against toxicological effect on non-target organisms.

scholarly journals Assessment of the growth performance and haematological indices of Clarias gariepinus fingerlings exposed to soap effluent

2021 ◽  
Vol 82 (1) ◽  
Author(s):  
Samuel Olusayo Akinkuolie ◽  
Henry Adefisayo Adewole ◽  
Promise Olabode Ololade ◽  
Bola Morufat Lawal ◽  
Victor Folorunso Olaleye
Keyword(s):  
Growth Performance ◽  
Clarias Gariepinus ◽  
Industrial Effluents ◽  
The Body ◽  
Control Fish ◽  
Performance Indices ◽  
Growth And Survival ◽  
Exposed Fish ◽  
Food Fish ◽  
Haematological Indices

Abstract Background Various substances released from modern complex human societies which enter the aquatic ecosystems produce alterations in survivability of aquatic biota. This study assesses the consequence of exposure to industrial effluents containing potentially hazardous constituents, especially soap industrial waste effluent, relative to fish physiology, growth and survival, particularly food fish such as Clarias gariepinus. Five hundred (500) fingerlings of C. gariepinus (15.65 ± 0.02 g) randomly stocked at 50 fish per tank in duplicates were exposed to varying concentration (0, 0.32, 0.66, 1.31 and 2.63%) of homogenous effluent sample and fed Durante® floating feed at 5% of the body weight in two instalments per day for 180 days. The growth performance and feed utilization data were generated to determine the growth performance indices. The haematological parameters of the fish were also determined following routine methods of fish haematology. Result Generally, fish in the control had significantly (p < 0.05) better growth performance indices compared with the exposed fish. It was also observed that most of the significant increase or decrease observed in the growth performance was concentration dependent. Increase in HES concentration which significantly increases (p < 0.05) WBC count of the experimental fish was observed to decrease (p < 0.05) the HGB level of the fish. Significantly higher (p < 0.05) levels of RBC (3.02 ± 0.03 × 106/µL) and HCT (36.70 ± 1.68%) were recorded in the control fish. However, all the exposed fish had significantly higher (p < 0.05) levels of platelet count compared with the control. Conclusion This study concluded that exposure to concentrations of soap effluent induced stress, inhibited growth and altered the haematological indices of the exposed fish. Uncontrolled discharge of soap effluent into receiving water bodies, apart from affecting the wellbeing of an important freshwater food fish, there is every probability of possible accumulation of the chemical/toxic additives of the soap effluent which could have serious implications considering the man as the major and final recipient of these toxic bioaccumulated chemicals via the food chain and the environment.

PHYTOCHEMICAL SCREENING AND LARVICIDAL EFFECT EVALUATION OF AZADIRACHTA INDICA (L) LEAF EXTRACT ON DERMESTES MACULATUS (DE GEER, 1774) INFESTATION OF SMOKED CLARIAS GARIEPINUS (BURCHELL, 1822)

2020 ◽  
Vol 4 (2) ◽  
pp. 41-45
Author(s):  
Sadam Sulaiman Indabo ◽  
Rufai Zakari
Keyword(s):  
Azadirachta Indica ◽  
Leaf Extract ◽  
Standard Procedure ◽  
Clarias Gariepinus ◽  
Phytochemical Screening ◽  
Range Finding ◽  
Dermestes Maculatus ◽  
Randomized Design ◽  
Fish Samples ◽  
Initial Source

Dermestes maculatus is a major pest of stored fish in Nigeria, fish is preserved with highly persistent synthetic chemicals. There have been an increasing effort at developing plant-based toxicants that are environmentally friendly. Therefore, this study was conducted to screen the leaf extract of Azadirachta indica for phytochemical constituents. The efficacy of the methanolic leaf extract of the plant against D. maculatus was also evaluated. The leaves of A. indica were obtained, processed and taken to laboratory for methanolic extraction. Phytochemical screening was carried out to identify saponins, flavonoids, alkaloids, tannins, steroids, cardiac glycosides, glycosides, triterpenes and carbohydrates using standard procedure.  Both clean and infested samples of smoke-dried Clarias gariepinus were purchased from Sabon Gari Market, Zaria. The infested samples were used as initial source of D. maculatus larvae. Range finding test was carried out prior to the bioassay. During the bioassay, clean un-infested fish samples were weighed and introduced into kilner jars and respective dosages of 0.2g, 0.4g, 0.6g and 0.8g of extract were added. Another set of fish samples without the extract were maintained as control. The experiment was arranged in Completely Randomized Design with three replicates. Fifteen larvae of D. maculatus were introduced into both treated and control containers and covered. Mortality was recorded after 24, 48, 72 and 96 hours. Data obtained revealed an increase in mortality with increasing dose and time. Mortality ranged from 1.67 to 11.00. Therefore, leaf extract of A. indica should be incorporated in the development of larvicide against D. maculatus.   

scholarly journals Determination of essential and non-essential element contents of drinking water and baby water for infant’s nutrition

2021 ◽  
Vol 44 (1) ◽  
pp. 194-202
Author(s):  
Funda Demir ◽  
Meral Yildirim Ozen ◽  
Emek Moroydor Derun
Keyword(s):  
Water Samples ◽  
Infant Nutrition ◽  
Tap Water ◽  
Essential Element ◽  
Essential Elements ◽  
Local Market ◽  
Drinking Waters ◽  
Significant Difference ◽  
Element Contents

Abstract In this study, essential (Ca, Cr, Cu, Fe, K, Mg, Na, P, Zn), and non-essential (Al, Ni, Pb) element contents of the drinking and baby water samples which are sold in the local market and tap water samples in Istanbul were examined. It was determined that elements of Cr, Cu, Fe, P, Zn, Al, and Ni were below detection limits in all water samples. Among the non-essential elements analyzed in water samples, Pb was the only detected element. At the same time, the percentages that meet the daily element requirements of infants were also calculated. As a result of the evaluations made, there is no significant difference in infant nutrition between baby waters and other drinking waters in terms of the element content.

scholarly journals Seasonal and Temperature Dependent Variations in Proximate Parameters and Mineral Elements of Smoke-Dried Catfish (Clarias gariepinus, Burchell, 1822) using Traditional and Eco-Friendly Kilns in Lagos, Nigeria

2020 ◽  
Vol 24 (4) ◽  
pp. 639-643
Author(s):  
M.M. Ogunbambo
Keyword(s):  
Crude Protein ◽  
Clarias Gariepinus ◽  
Mineral Elements ◽  
Drying Process ◽  
Temperature Dependent ◽  
Crude Fibre ◽  
Significant Difference ◽  
Dry Seasons ◽  
Mineral Compositions ◽  
Wet And Dry Seasons

Smoke-drying Clarias gariepinus (catfish) provides animal protein and a source of livelihood in Lagos, Nigeria. Changes occurring to seasonal and mineral compositions of smoke-dried catfish using local Traditional Drum Kiln (TDK) and a newly constructed Eco-Friendly Kiln (EFK) fitted with a flame, drying and electronic components was carried out in this study. The smoke-drying process was carried out in both wet and dry seasons and smoke-dried catfish samples stored at ambient and adjusted refrigerated temperatures of 28 and 4 0C. The smoke-drying procedure was  standardized at 60 - 80 0C and kiln lasted 24 ± 3 hours. Moisture content results showed a significant difference when the catfish samples were smoke-dried using TDK and EFK and stored in ambient and controlled temperatures in both wet and dry seasons while crude protein, lipid, ash and crude fibre values were significantly different when stored only in dry season. Mineral elements phosphorus, sodium, copper, magnesium and iron showed a significant difference when stored at both temperatures and seasons using both kilns. Mineral elements were found to be most stable in smoke-dried catfish samples when stored at controlled temperatures. This work proved that standardizing smoke-drying process using both kilns resulted in good quality smoke-dried catfish but showed that higher biochemical values were obtained when EFK is used. Key words: Nigeria, Smoke-drying Kilns, Seasons, Ambient, Controlled Temperatures

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